CINXE.COM

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class="region region-content"> <div id="block-et-messages-et-messages-block" class="block block-et-messages"> <div class="content"> <span></span> </div> </div> <div id="block-system-main" class="block block-system"> <div class="content"> <div id="node-31469446" class="node node-page clearfix"> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><table border="0" cellpadding="6" cellspacing="6"> <tr> <td colspan="2"><img src="https://images.novusbio.com/design/Flow-cy-banner2.jpg" alt="Flow Cytometry Applications" > </td> </tr> <tr> <td width="264" valign="top" style="width:220px;"> <p><b><u>Related Links</u></b></p> <p><a href="https://info.bio-techne.com/flow-cytometry-handbook.html?utm_source=novusbio.com&utm_medium=banner&utm_campaign=Flow%20Cytometry%20handbook&utm_term=applications%20flow%20cytometry&utm_content=banner" target="_blank">Flow Cytometry Handbook</a></p> <p><a href="/support/flow-cytometry-resources">Flow Cytometry Protocols, Troubleshooting, & Scientific Resources</a></p> <p><a href="/spectraviewer">Spectra Viewer</a> </p> <p><a href="/novusknowsflow.html">Flow Cytometry Panel Builder</a> </p> <p><a href="/products/antibodies-cytof">Mass Cytometry/CyTOF</a></p> <p><a href="https://www.bio-techne.com/resources/literature/human-immune-cell-marker-poster?utm_source=novusbio.com&utm_medium=referral&utm_campaign=sister-link&utm_term=flow%20cytometry%20application&utm_content=flow%20cytometry" target="_blank">Cell Markers Guide</a></p> <p><a href="/application/single-cell-western">Single-Cell Western</a></p> </td> <td width="667"> <p>Flow cytometry uses fluorescently-labeled molecules to identify and characterize antigen profiles in a variety of research applications and for clinical diagnostics on a single-cell basis. By using different fluorochromes with emission wavelengths that span across the color spectrum, proteins associated with surface, cytosolic, and nuclear compartments can be detected simultaneously. Through advances in instrument design and the development of new fluorescent probes, cytometers can now measure more than 20 parameters using multi-color panels to identify and phenotype cells involved in complex biological processes. </p> <p align="center"> <img src="https://images.novusbio.com/design/flow-machine-2.jpg" alt="Flow Cytometry Design" title="Flow Cytometry Design" > </p> <p><em>The fluidics system moves cells from the sample tube to the flow cell where the sample stream is focused for single cell analysis. The optics system uses lasers as a source of excitation for fluorescent probes, where photons and light scatter are directed to detectors via fiber optics or lenses/mirrors. The electronics system converts the emitted photons into a voltage pulse over time as output with one- and two-dimensional plots. <a href="https://www.novusbio.com/support/support-by-application.html">Learn more about Flow Cytometry</a>.</em></p> <br/><br/> <h2>Flow Cytometry Primary Antibodies</h2> <p>Novus Biologicals offers more than 8,000 flow cytometry validated primary antibodies and over 2,500 CyTOF-ready antibodies backed by our <a href="/support/quality-assurance.html">100% guarantee</a>. With nearly 20 different fluorescent conjugates available including a range of Alexa Fluor<sup>®</sup> and tandem dyes, the breadth of antibody-dye options available are compatible with an array of cytometer configurations and are further expanded by our custom antibody labeling services. At Novus, we understand how important it is to provide high quality reagents. <a href="/5-pillars-validation">Learn more about Bio-Techne’s methods for antibody validation.</a></p> <table> <tr> <td colspan="2" valign="top"> <b>Choose from:</b> </td> </tr> <tr> <td valign="top"> <ul> <li> <a href="/product-type/kits?kit_type=Antibody%20Labeling%20Kit">Antibody Labeling Kits</a></li> <li><a href="/Custom%20Conjugation%20Services">Custom Conjugation Service</a><br> </li> <li><a href="/product-type/primary-antibodies?applications=Flow%20Cytometry|Knockout%20Validated">Knockout Validated Antibodies for Flow Cytometry</a><br> </li> <li><a href="/product-type/primary-antibodies?applications=Flow%20Cytometry&facet_conjugate=Alexa%20Fluor%20405">Primary Antibodies – Alexa Fluor<sup>®</sup> 405</a> <br> </li> <li><a href="/product-type/primary-antibodies?applications=Flow%20Cytometry&facet_conjugate=PE">Primary Antibodies – PE</a></li> </ul> </td> <td valign="top"> <ul> <li> <a href="/product-type/primary-antibodies?applications=Flow%20Cytometry&facet_conjugate=PE/Atto594">Primary Antibodies – PE/Atto594</a></li> <li><a href="/search?keywords=novus&applications=CyTOF-ready&category=Primary%20Antibodies">CyTOF-ready Antibodies</a><br> </li> <li><a href="/search?keywords=novus&category=Primary%20Antibodies&applications=CyTOF-reported">CyTOF-reported Antibodies</a></li> <li><a href="/products/janelia-fluor"> Janelia Fluor<sup>®</sup> Dyes for live-cell imaging </a></li> <li><a href="/search?keywords=novus&category=Primary%20Antibodies&applications=Flow%20Cytometry">See all primary antibodies, validated for flow cytometry</a> </li> </ul></td> <tr> </table> <br/><br/> <p align="center"> <a href=" https://www.bio-techne.com/clinical-solutions/cell-and-gene-therapy-solutions?utm_source=novusbio.com&utm_medium=referral&utm_campaign=Flow%20application&utm_content=CGT" target="_blank"><img src="https://images.novusbio.com/design/cell-and-gene-therapy-Ad.jpg" alt="Pioneering Cell and Gene Therapy Solutions from Discovery to Clinic" title="Bio-Techne offers Cell and Gene Therapy Products" ></a> </p> <br/><br/> <h2>Flow Cytometry Secondary Antibodies</h2> <p>For <a href="/antibody-news/antibodies/the-key-benefits-of-indirect-detection">indirect detection</a>, a fluorescently-labeled secondary antibody detects the antigen-primary antibody complex. Compared to directly labeled primary antibodies, indirect detection is more sensitive and vital for effective identification of low abundance antigens and rare epitopes. Our secondary antibodies cover a variety of applications, hosts, and conjugates to match your research needs.</p> <table> <tr> <td colspan="2" valign="top"> <b>Choose from:</b> </td> </tr> <tr> <td width="302" valign="top"> <ul> <li> <a href="/product-type/secondary-antibodies?facet_conjugate=DyLight%20488">Secondary Antibodies - DyLight<sup>™</sup> 488</a></li> <li><a href="/product-type/secondary-antibodies?facet_conjugate=DyLight%20550">Secondary Antibodies - DyLight<sup>™</sup> 550 </a> </li> </ul> </td> <td width="350" valign="top"> <ul> <li> <a href="/product-type/secondary-antibodies?facet_conjugate=DyLight%20650">Secondary Antibodies - DyLight<sup>™</sup> 650 </a> <br> </li> <li><a href="/search?keywords=novus&category=Secondary%20Antibodies&applications=Flow%20Cytometry">See all secondary antibodies, validated for flow cytometry</a> </li> </ul> </td> <tr> </table> <br/><br/> <p align="center"> <a href="/spectraviewer"><img src="https://images.novusbio.com/design/we-bad_flow-cytometry-panel-builder.jpg" alt="Flow Cytometry Panel Builder tool" title="Streamline the Panel Design Process" border="0" ></a> </p> <br/><br/> <h2>Experimental Controls</h2> <p>Use of <a href="/products/isotype-controls">isotype controls</a>, compensation controls, or Fluorescence Minus One (FMO) controls alongside cell viability solutions minimize background staining, false positives and skewed results. When using fluorochrome conjugated antibodies, it is recommended that isotype controls are prepared alongside the primary antibody to ensure consistent labeling, thus minimizing impact from fluorochrome variability. To learn more about data reliability through use of controls, review our <a href="https://info.bio-techne.com/flow-cytometry-handbook.html?utm_source=novusbio.com&utm_medium=referral&utm_campaign=Flow%20application&utm_content=flow%20handbook " target="_blank">Flow Cytometry Handbook</a>. </p> <table> <tr> <td colspan="2" valign="top"> <b>Choose from:</b> </td> </tr> <tr> <td valign="top"> <ul> <li> <a href="/product-type/isotype-controls">Isotype Controls</a> <br> </li> <li><a href="/common-name/propidium-iodide">Propidium Iodide</a> <br> </li> </ul></td> <td valign="top"> <ul> <li><a href="/common-name/7-aminoactinomycin-d-solution">7-Aminoactinomycin D</a></li> <li><a href="/common-name/calcein-blue-am-solution"> Calcein Blue AM </a></li> </ul> </td> </tr> </table> <br/><br/> <p align="center"> <a href="https://www.proteinsimple.com/milo.html?utm_source=novusbio.com&utm_medium=referral&utm_campaign=sister-link&utm_content=application-flow-cytometry" target="_blank" ><img src="https://images.novusbio.com/design/PS-milo-ad2.jpg" alt="Milo - Single-Cell Western - Simplify FACS Studies" title="Single-cell Western Milo Instrument Compliments Flow Cytometry"></a> </p> <br/><br/> <h2>Supporting Products, Kits and Reagents</h2> <p>Our wide array of products validated for flow cytometry can be used to isolate, fix and/or enrich cell populations to help determine cell fate, responsiveness to <a href="/search?keywords=novus&applications=Flow%20Cytometry&category=Inhibitors">inhibitors</a> and assess cell functionality. </p> <table> <tr> <td colspan="2" valign="top"> <b>Choose from:</b> </td> </tr> <tr> <td valign="top"> <ul> <li> <a href="/product-type/kits?category=Kits&kit_type=Flow%20Cytometry%20Kit">Flow Cytometry Kits</a> <br> </li> <li><a href="/search?keywords=TUNEL%20assay%20kit&applications=Flow%20Cytometry&category=Kits">TUNEL Apoptosis Kits</a> <br> </li> <li><a href="/search?keywords=annexin%20v&applications=Flow%20Cytometry&category=Kits">Annexin V Apoptosis Kits</a> <br> </li> <li><a href="/products/psiva-apoptosis-detection-kit_nbp2-29611">pSIVA Apoptosis Detection Kit</a> <br> </li> <li> <a href="/search?keywords=isolation%20kits&applications=Flow%20Cytometry&category=Kits">Cell Isolation Kits</a> <br> </li> <li><a href="/search?keywords=enrichment%20kits&applications=Flow%20Cytometry&category=Kits">Cell Enrichment Kits</a> <br> </li> </ul></td> <td valign="top"> <ul> <li><a href="/search?keywords=Fixation%20and%20Permeabilization%20kits&applications=Flow%20Cytometry&category=Kits">Fixation and Permeabilization Kits</a> <br> </li> <li><a href="/product-type/peptides-and-proteins?applications=Bioactivity">Bioactive Proteins</a><br> </li> <li><a href="/products/cytrak-orange-tm-_nbp2-81127">CyTRAK Orange<sup>™</sup></a><br> </li> <li> <a href="/products/draq5-tm-_nbp2-81125">DRAQ5<sup>™</sup></a> (Live or fixed cells)<br> </li> <li><a href="/products/draq7-tm-_nbp2-81126">DRAQ7<sup>™</sup></a> (Fixed cells only) </li> </ul> 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