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In the developed method C18 column was used and a mixture of isopropanol and water was used as mobile phase to achieve baseline separation of all isomers. Tocopherols were separated completely with very good peak resolution in 45 minutes and were detected using diode array detector. No pretreatment of the samples was needed, except dilution in ethanol. The validation measurements showed that the developed method was suitable for quantification of tocopherols in edible oils.","publication_date":"2018,,","publication_name":"Egyptian Journal of Chemistry","grobid_abstract_attachment_id":"85640981"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Development and Validation of Simple Reversed Phase Method for Separation and Quantification of Tocopherols in Edible Oils","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [131411281]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loginModal = {}; window.loginModal.appleClientId = 'edu.academia.applesignon'; window.userInChina = "false";</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":85640981,"attachmentType":"pdf"}"><img alt="First page of “Development and Validation of Simple Reversed Phase Method for Separation and Quantification of Tocopherols in Edible Oils”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/85640981/mini_magick20220527-5005-11pnflr.png?1653701007" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/images/single_work_splash/adobe_icon.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Development and Validation of Simple Reversed Phase Method for Separation and Quantification of Tocopherols in Edible Oils</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="131411281" href="https://independent.academia.edu/AdelShehata4"><img alt="Profile image of Adel Shehata" class="ds-work-card--author-avatar" src="https://0.academia-photos.com/131411281/43274275/34444933/s65_adel.shehata.jpg" />Adel Shehata</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2018, Egyptian Journal of Chemistry</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">8 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 78676861; 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In the developed method C18 column was used and a mixture of isopropanol and water was used as mobile phase to achieve baseline separation of all isomers. Tocopherols were separated completely with very good peak resolution in 45 minutes and were detected using diode array detector. No pretreatment of the samples was needed, except dilution in ethanol. The validation measurements showed that the developed method was suitable for quantification of tocopherols in edible oils.</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":85640981,"attachmentType":"pdf","workUrl":"https://www.academia.edu/78676861/Development_and_Validation_of_Simple_Reversed_Phase_Method_for_Separation_and_Quantification_of_Tocopherols_in_Edible_Oils"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":85640981,"attachmentType":"pdf","workUrl":"https://www.academia.edu/78676861/Development_and_Validation_of_Simple_Reversed_Phase_Method_for_Separation_and_Quantification_of_Tocopherols_in_Edible_Oils"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div><div class="ds-signup-banner-trigger-container"><div class="ds-signup-banner-trigger ds-signup-banner-trigger-control"></div></div><div class="ds-signup-banner ds-signup-banner-control"><div id="ds-signup-banner-close-button"><button class="ds2-5-button ds2-5-button--secondary ds2-5-button--inverse"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">close</span></button></div><div class="ds-signup-banner-ctas" data-impression-entity-id="78676861" data-impression-entity-type="2" data-impression-source="signup-banner"><img src="//a.academia-assets.com/images/academia-logo-capital-white.svg" /><h4 class="ds2-5-heading-serif-sm">Sign up for access to the world's latest research</h4><button class="ds2-5-button ds2-5-button--inverse ds2-5-button--full-width js-swp-download-button" data-signup-modal="{"location":"signup-banner"}">Sign up for free<span class="material-symbols-outlined" style="font-size: 20px" translate="no">arrow_forward</span></button></div><div class="ds-signup-banner-divider"></div><div class="ds-signup-banner-reasons"><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Get notified about relevant papers</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Save papers to use in your research</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Join the discussion with peers</span></div><div class="ds-signup-banner-reasons-item"><span class="material-symbols-outlined" style="font-size: 24px" translate="no">check</span><span>Track your impact</span></div></div></div><script>(() => { // Set up signup banner show/hide behavior: // 1. 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The structure of the compounds was identical, so it could not be separated by reverse phase liquid chromatography. The obtained peaks show an important overlapping and we have used chemometric tools to get a calibration model, which assumes a linear behavior, i.e., the total signal is the sum of individual signals of components. Recovery percentages were between 90.3% and 95.7%. The method has been successfully applied to edible oils and dietary supplements. No pretreatment of the samples was needed.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Development of an Advance Method for the Determination of Tocopherols in Edible Oils","attachmentId":115676043,"attachmentType":"pdf","work_url":"https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="40347682" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography">Extraction and quantification of tocopherols from edible oils using high performance liquid chromatography</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="126411885" href="https://salu.academia.edu/MSirohi">Muzafar H . Sirohi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Biosciences, 2019</p><p class="ds-related-work--abstract ds2-5-body-sm">Adulteration of Extra Virgin Olive Oil with olive oil and sunflower oil remained major issue in edible oil industry. Herein, rapid, sensitive and precise method for the determination of all type of tocopherols in different vegetable oils includinhg sunflower oil, olive oil and extra virgin olive oil is reported. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) with UV detector was used for tocopherol determination. A simple, quick and sensitive method to estimate the antioxidant quantity is proposed. In this method oils were diluted in methanol and injected directly into column (no saponification procedure). Methanol and water (98:2) mixture was used as mobile phase. Three tocopherols (α, γ and δ) were detected at 292 nm wavelength with UV detector. Method had good limit of detection (LOD) (7ng/g) and reproducibility (C.V% 0.9, 0.8 and 0.4 for α, γ and δ tocopherols, respectively). Result showed the best source for α-tocopherol was sun flower oil (146.65±1.7mg/kg). Oil richest in δ-tocopherol was olive oil and δ-tocopherol was absent in extra virgin olive oil. The current study suggest the new parameter (ratio of α/γ) as first screening indicator of authenticity of purity of extra virgin olive oil for differentiation of various cultivators of same generic source.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Extraction and quantification of tocopherols from edible oils using high performance liquid chromatography","attachmentId":60593264,"attachmentType":"pdf","work_url":"https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/40347682/Extraction_and_quantification_of_tocopherols_from_edible_oils_using_high_performance_liquid_chromatography"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="11938642" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/11938642/Identification_and_quantification_of_tocopherols_in_vegetable_oils_by_thin_layer_chromatography">Identification and quantification of tocopherols in vegetable oils by thin-layer chromatography</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="29585141" href="https://independent.academia.edu/ClaudiaCimpoiu">Claudia Cimpoiu</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="237394676" href="https://independent.academia.edu/CasoniDorina">Dorina Casoni</a></div><p class="ds-related-work--metadata ds2-5-body-xs">JPC-Journal of Planar …, 2008</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Identification and quantification of tocopherols in vegetable oils by thin-layer chromatography","attachmentId":46429353,"attachmentType":"pdf","work_url":"https://www.academia.edu/11938642/Identification_and_quantification_of_tocopherols_in_vegetable_oils_by_thin_layer_chromatography","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/11938642/Identification_and_quantification_of_tocopherols_in_vegetable_oils_by_thin_layer_chromatography"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="64396987" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/64396987/Validation_of_Reversed_phase_High_performance_Liquid_Chromatography_Method_for_Determination_of_Alpha_Tocopherol_in_Corn_Oil">Validation of Reversed-phase High-performance Liquid Chromatography Method for Determination of Alpha Tocopherol in Corn Oil</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="7376011" href="https://poliupg.academia.edu/LenyIrawati">Leny Irawati</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2017</p><p class="ds-related-work--abstract ds2-5-body-sm">Determination of alpha tocopherol in corn oil can be analyzed by using Reversed-Phase High Performance Liquid Chromatography (RP-HPLC). This research aimed to validate RP-HPLC method for determination of alpha tocopherol in corn oil. The method was based on linearity, precision, accuracy, limit detection (LOD) and limit quantization (LOQ). The Reversed Phase High Performance Liquid Chromatography (RP-HPLC) method includes the determination of the wavelength, the mobile phase composition and the flow rate of mobile phase, then validation of each of the above methods. The maximum wavelength was found in 290 nm, composition of mobile phase was 100% methanol, flow rate of mobile phase was 1,5 mL/min and retention time was 9,67 min. The researched result indicated that the method of RP-HPLC had a linearity with the correlation coefficient of 0.999, a relative standard deviation (%RSD) for intra and inter-day precision was &lt; 5%, accuracy/recovery was 95.69-100.99 %, detection limit wa...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Validation of Reversed-phase High-performance Liquid Chromatography Method for Determination of Alpha Tocopherol in Corn Oil","attachmentId":76453110,"attachmentType":"pdf","work_url":"https://www.academia.edu/64396987/Validation_of_Reversed_phase_High_performance_Liquid_Chromatography_Method_for_Determination_of_Alpha_Tocopherol_in_Corn_Oil","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/64396987/Validation_of_Reversed_phase_High_performance_Liquid_Chromatography_Method_for_Determination_of_Alpha_Tocopherol_in_Corn_Oil"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="34385558" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/34385558/Analysis_of_tocopherols_in_vegetable_oils_by_high_performance_liquid_chromatography_Comparison_of_fluorescence_and_evaporative_light_scattering_detection">Analysis of tocopherols in vegetable oils by high-performance liquid chromatography: Comparison of fluorescence and evaporative light-scattering detection</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34694729" href="https://uga.academia.edu/wwwcaesugaedudepartmentsfstLBCAP">Casimir C Akoh</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of the American Oil Chemists’ Society, 1994</p><p class="ds-related-work--abstract ds2-5-body-sm">A comparison of the responses of an evaporative lightscattering detector (ELSD) and a fluorescence detector for tocopherols in vegetable oils by high-performance liquid chromatography is presented. The tocopherols were separated from acylglycerols by gel-permeation chromatography (GPC). The tocopherol fraction was collected off a set of four GPC columns with a mobile phase of methylene chloride before separation on a normal-phase silica column with a mobile phase of hexane/isopropanol, 99.7:0.3 {vol/vol). An internal standard of 5,7 dimethyltocol, which was detected by both the ELSD and fluorescence detector, was used to obtain quantitative data. The fluorescence detector was ten times more sensitive than the ELSD. y-Tocopherol was the major tocopherol detected in the vegetable oils studied and ranged from 24.1-93.3 mg/100 g. The amounts of tocopherols found in the vegetable oils agreed favorably with the literature values.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Analysis of tocopherols in vegetable oils by high-performance liquid chromatography: Comparison of fluorescence and evaporative light-scattering detection","attachmentId":54270951,"attachmentType":"pdf","work_url":"https://www.academia.edu/34385558/Analysis_of_tocopherols_in_vegetable_oils_by_high_performance_liquid_chromatography_Comparison_of_fluorescence_and_evaporative_light_scattering_detection","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/34385558/Analysis_of_tocopherols_in_vegetable_oils_by_high_performance_liquid_chromatography_Comparison_of_fluorescence_and_evaporative_light_scattering_detection"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="61393411" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/61393411/Separation_and_determination_of_tocopherols_in_vegetable_oils_by_solid_phase_extraction_on_porous_polymers_SPE_cartridges_and_capillary_gas_chromatography_">Separation and determination of tocopherols in vegetable oils by solid phase extraction on porous polymers SPE cartridges and capillary gas chromatography …</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="42735394" href="https://independent.academia.edu/VComan1">V. Coman</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Central European Journal of …</p><p class="ds-related-work--abstract ds2-5-body-sm">Vitamin E is a generic term that defines a family of organic compounds with similar structure consisting of a chromanol core and an aliphatic chain named phythol. In nature, vitamin E exists in eight different forms: α-, β-, γand δ-tocopherol and α-, β-, γ-and δ-tocotrienol. These tocopherols have been intensively studied due to their medical, biological and physiochemical significance. Usually, the role of vitamin E inside human body is to prevent unwanted oxidative processes and to stop the formation of free radicals [1]. Humans, as well as a considerable number of animal species, do not synthesize their own vitamin E, but they acquire this vitamin from a large variety of nutrients. The absence of vitamin E from nourishment leads to neuropathy, fetal death or musclular diseases and is directly connected to different regulatory processes [2]. Based on their critical significance, it is very important to design techniques able to extract tocopherols efficiently from various sources and analyze them with high accuracy and precision. The most commonly used analytical techniques for tocopherol analyses include spectrophotometry [3,4], voltametry [5,6], thinlayer chromatography, gas chromatography and high performance liquid chromatography [7-25]. Even though the tocopherols are not chemically bonded to the proteins, lipids or carbohydrates, they could be assosciated with other components of food matrices such as membranes, lipoproteins etc. [1,2]. There are two major sample preparation approaches for food samples: direct extraction of the tocopherols from</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Separation and determination of tocopherols in vegetable oils by solid phase extraction on porous polymers SPE cartridges and capillary gas chromatography …","attachmentId":74437536,"attachmentType":"pdf","work_url":"https://www.academia.edu/61393411/Separation_and_determination_of_tocopherols_in_vegetable_oils_by_solid_phase_extraction_on_porous_polymers_SPE_cartridges_and_capillary_gas_chromatography_","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/61393411/Separation_and_determination_of_tocopherols_in_vegetable_oils_by_solid_phase_extraction_on_porous_polymers_SPE_cartridges_and_capillary_gas_chromatography_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="24566914" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/24566914/Analysis_of_Tocopherols_by_High_Performance_Liquid_Chromatography">Analysis of Tocopherols by High Performance Liquid Chromatography</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="47356103" href="https://independent.academia.edu/MiguelCueva6">Miguel Cueva</a></div><p class="ds-related-work--abstract ds2-5-body-sm">Gas chromatography is the key technique for organic components and also for tocopherols analysis. High performance liquid chromatography has an important role to take part in applications such as the handling of less usual samples, prevention of degradation of heat sensitive functional groups and for micro preparative purposes. Many approaches for development of improved methods are suggested, especially for reversed phase applications.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Analysis of Tocopherols by High Performance Liquid Chromatography","attachmentId":44896177,"attachmentType":"pdf","work_url":"https://www.academia.edu/24566914/Analysis_of_Tocopherols_by_High_Performance_Liquid_Chromatography","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/24566914/Analysis_of_Tocopherols_by_High_Performance_Liquid_Chromatography"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="121124775" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/121124775/Tocopherols_and_Tocotrienols_an_Adapted_Methodology_by_UHPLC_MS_Without_Sample_Pretreatment_Steps">Tocopherols and Tocotrienols: an Adapted Methodology by UHPLC/MS Without Sample Pretreatment Steps</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="104600906" href="https://independent.academia.edu/MarinaAnsolin">Marina Ansolin</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Food Analytical Methods, 2017</p><p class="ds-related-work--abstract ds2-5-body-sm">Ultra high-performance liquid chromatographyelectrospray ionization-mass spectrometry (UHPLC/ESI/ MS) methodology was adapted for identification and quantification of tocopherols and tocotrienols in vegetable oils with no derivatization or sample preparation steps. The UHPLC analysis was performed using a C18 column and mobile phase composed of methanol: water: ammonium hydroxide (99:1:0.1 v/v/v) and isopropanol. A single mass spectrometer with electrospray on negative mode was used as a detector for tocopherols and tocotrienols. The samples were diluted in isopropanol. The limit of quantification for tocopherols was 0.006 μg mL −1 , and the linear range was 0.006 to 0.01 μg mL −1 ; for tocotrienols, the limit of quantification was 0.002 μg mL −1 , and the linear range of analysis was 0.002 to 0.003 μg mL −1. The correlation coefficients were higher than 0.99, indicating that the method has suitable linearity. The methodology has proven to be precise, reproducible, and robust for the parameters studied.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Tocopherols and Tocotrienols: an Adapted Methodology by UHPLC/MS Without Sample Pretreatment Steps","attachmentId":116089849,"attachmentType":"pdf","work_url":"https://www.academia.edu/121124775/Tocopherols_and_Tocotrienols_an_Adapted_Methodology_by_UHPLC_MS_Without_Sample_Pretreatment_Steps","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/121124775/Tocopherols_and_Tocotrienols_an_Adapted_Methodology_by_UHPLC_MS_Without_Sample_Pretreatment_Steps"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="2297970" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/2297970/Comparison_of_reversed_phase_liquid_chromatography_mass_spectrometry_with_electrospray_and_atmospheric_pressure_chemical_ionization_for_analysis_of_dietary_tocopherols">Comparison of reversed-phase liquid chromatography–mass spectrometry with electrospray and atmospheric pressure chemical ionization for analysis of dietary tocopherols</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2389241" href="https://independent.academia.edu/JelenaJastrebova">Jelena Jastrebova</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2007</p><p class="ds-related-work--abstract ds2-5-body-sm">ESI and APCI ionization techniques in both negative and positive ion modes were evaluated for simultaneous LC–MS analysis of the four tocopherol homologues (α, β, γ and δ). The ESI and APCI ionization of tocopherols in positive ion mode was not efficient and proceeded via two competitive mechanisms, with the formation of protonated pseudo-molecular ions and molecular ions, which adversely influenced the repeatability of MS signal. Ionization in negative ion mode in both ESI and APCI was more efficient as it only produced target deprotonated pseudo-molecular ions. The APCI in negative ion mode showed larger linearity range, lower detection limits and was less sensitive to the differences in chemical structure of analytes and nature of applied solvents than negative ion ESI. Negative ion APCI was, therefore, chosen for the development of LC–MS method for simultaneous determination of the four tocopherols in foods. A baseline separation of the tocopherols was achieved on novel pentafluorophenyl silica-based column Fluophase PFP. The use of methanol–water (95:5, v/v) as a mobile phase was preferable to the use of acetonitrile–water due to considerable gain in MS signal. The limits of quantifications were 9 ng/mL for α-tocopherol, 8 ng/mL for β- and γ- and 7.5 ng/mL for δ-tocopherol when 2 μL was injected. This method was successfully applied to determination of tocopherols in sunflower oil and milk.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Comparison of reversed-phase liquid chromatography–mass spectrometry with electrospray and atmospheric pressure chemical ionization for analysis of dietary tocopherols","attachmentId":31635986,"attachmentType":"pdf","work_url":"https://www.academia.edu/2297970/Comparison_of_reversed_phase_liquid_chromatography_mass_spectrometry_with_electrospray_and_atmospheric_pressure_chemical_ionization_for_analysis_of_dietary_tocopherols","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/2297970/Comparison_of_reversed_phase_liquid_chromatography_mass_spectrometry_with_electrospray_and_atmospheric_pressure_chemical_ionization_for_analysis_of_dietary_tocopherols"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="10935299" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/10935299/Rapid_Determination_of_%CE%B1_Tocopherol_in_Vegetable_Oils_by_Fourier_Transform_Infrared_Spectroscopy">Rapid Determination of α-Tocopherol in Vegetable Oils by Fourier Transform Infrared Spectroscopy</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="26513486" href="https://independent.academia.edu/NunoRosa2">Nuno Rosa</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Food Analytical Methods, 2009</p><p class="ds-related-work--abstract ds2-5-body-sm">The aim of this study was to develop a rapid methodology for the analysis of α-tocopherol in vegetable oils as an alternative to the high-performance liquid chromatography (HPLC) methods: Fourier transform infrared (FT-IR) methodology. Thirteen vegetable oils (corn, peanut, soybean, sunflower and mixtures) commercially obtained were analysed by reverse-phase HPLC with fluorescence detection (FD) in order to obtain standard values for α-tocopherol. Validation tests were performed concerning the HPLC method. The HPLC method is valid for α-tocopherol analysis in the 1-90 mg/L linear range.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Rapid Determination of α-Tocopherol in Vegetable Oils by Fourier Transform Infrared Spectroscopy","attachmentId":47014291,"attachmentType":"pdf","work_url":"https://www.academia.edu/10935299/Rapid_Determination_of_%CE%B1_Tocopherol_in_Vegetable_Oils_by_Fourier_Transform_Infrared_Spectroscopy","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/10935299/Rapid_Determination_of_%CE%B1_Tocopherol_in_Vegetable_Oils_by_Fourier_Transform_Infrared_Spectroscopy"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":85640981,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":85640981,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_85640981" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="54031424" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/54031424/Physicochemical_Characteristics_and_HPLC_Determination_of_Alpha_Tocopherol_in_Eighteen_Edible_Vegetable_Oils_Marketed_in_Nigeria">Physicochemical Characteristics and HPLC Determination of Alpha-Tocopherol in Eighteen Edible Vegetable Oils Marketed in Nigeria</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2660325" href="https://oou.academia.edu/olufunmilayoadejumo">olufunmilayo adejumo</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Dhaka University Journal of Pharmaceutical Sciences</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Physicochemical Characteristics and HPLC Determination of Alpha-Tocopherol in Eighteen Edible Vegetable Oils Marketed in Nigeria","attachmentId":70591840,"attachmentType":"pdf","work_url":"https://www.academia.edu/54031424/Physicochemical_Characteristics_and_HPLC_Determination_of_Alpha_Tocopherol_in_Eighteen_Edible_Vegetable_Oils_Marketed_in_Nigeria","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/54031424/Physicochemical_Characteristics_and_HPLC_Determination_of_Alpha_Tocopherol_in_Eighteen_Edible_Vegetable_Oils_Marketed_in_Nigeria"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="1" data-entity-id="48590591" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/48590591/Chemometrics_for_the_resolution_of_co_eluting_peaks_of_%CE%B2_and_%CE%B3_tocopherols_in_RP_HPLC_Determination_in_edible_oils_and_dietary_supplements">Chemometrics for the resolution of co-eluting peaks of β- and γ-tocopherols in RP-HPLC: Determination in edible oils and dietary supplements</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="43383257" href="https://independent.academia.edu/TGaleanod%C3%ADaz">T. Galeano-díaz</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Food Chemistry, 2007</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Chemometrics for the resolution of co-eluting peaks of β- and γ-tocopherols in RP-HPLC: Determination in edible oils and dietary supplements","attachmentId":67123669,"attachmentType":"pdf","work_url":"https://www.academia.edu/48590591/Chemometrics_for_the_resolution_of_co_eluting_peaks_of_%CE%B2_and_%CE%B3_tocopherols_in_RP_HPLC_Determination_in_edible_oils_and_dietary_supplements","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/48590591/Chemometrics_for_the_resolution_of_co_eluting_peaks_of_%CE%B2_and_%CE%B3_tocopherols_in_RP_HPLC_Determination_in_edible_oils_and_dietary_supplements"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="2" data-entity-id="63880756" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/63880756/Tocopherol_content_in_vegetable_oils_using_a_rapid_HPLC_fluorescence_detection_method">Tocopherol content in vegetable oils using a rapid HPLC fluorescence detection method</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="179288064" 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ds2-5-body-xs">Open Chemistry, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Separation and determination of tocopherols in vegetable oils by solid phase extraction on porous polymers SPE cartridges and capillary gas chromatography analysis","attachmentId":103619052,"attachmentType":"pdf","work_url":"https://www.academia.edu/103674194/Separation_and_determination_of_tocopherols_in_vegetable_oils_by_solid_phase_extraction_on_porous_polymers_SPE_cartridges_and_capillary_gas_chromatography_analysis","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" 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Simó-alfonso</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33933893" href="https://independent.academia.edu/LorenzoCerretani">Lorenzo Cerretani</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Agricultural and Food Chemistry, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Determination of Tocopherols and Tocotrienols in Vegetable Oils by Nanoliquid Chromatography with Ultraviolet−Visible Detection Using a Silica Monolithic Column","attachmentId":43722755,"attachmentType":"pdf","work_url":"https://www.academia.edu/14942654/Determination_of_Tocopherols_and_Tocotrienols_in_Vegetable_Oils_by_Nanoliquid_Chromatography_with_Ultraviolet_Visible_Detection_Using_a_Silica_Monolithic_Column","alternativeTracking":true}"><span class="material-symbols-outlined" 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pentafluorophenyl bonded phase for analysis of tocopherols </a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="11969422" href="https://independent.academia.edu/CodyBeaumont">Cody Beaumont</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Reversed-phase hplc method using a pentafluorophenyl bonded phase for analysis of tocopherols ","attachmentId":34629830,"attachmentType":"pdf","work_url":"https://www.academia.edu/8200025/Reversed_phase_hplc_method_using_a_pentafluorophenyl_bonded_phase_for_analysis_of_tocopherols","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline 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href="https://independent.academia.edu/PatriciaOliveira116">Patricia Oliveira</a></div><p class="ds-related-work--metadata ds2-5-body-xs">J Agr Food Chem, 2006</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Quantification of Tocopherols and Tocotrienols in Portuguese Olive Oils Using HPLC with Three Different Detection Systems","attachmentId":43690954,"attachmentType":"pdf","work_url":"https://www.academia.edu/23202164/Quantification_of_Tocopherols_and_Tocotrienols_in_Portuguese_Olive_Oils_Using_HPLC_with_Three_Different_Detection_Systems","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/23202164/Quantification_of_Tocopherols_and_Tocotrienols_in_Portuguese_Olive_Oils_Using_HPLC_with_Three_Different_Detection_Systems"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="8" data-entity-id="20465132" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/20465132/Tocopherol_speciation_as_first_screening_for_the_assessment_of_extra_virgin_olive_oil_quality_by_reversed_phase_high_performance_liquid_chromatography_fluorescence_detector">Tocopherol speciation as first screening for the assessment of extra virgin olive oil quality by reversed-phase high-performance liquid chromatography/fluorescence detector</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="42677838" href="https://independent.academia.edu/GiuseppeSalvetti">Giuseppe Salvetti</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="41892199" href="https://independent.academia.edu/MAngiuli">M. Angiuli</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2360454" href="https://cnr-it.academia.edu/EmiliaBramanti">Emilia Bramanti</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Food Chemistry, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Tocopherol speciation as first screening for the assessment of extra virgin olive oil quality by reversed-phase high-performance liquid chromatography/fluorescence detector","attachmentId":41388432,"attachmentType":"pdf","work_url":"https://www.academia.edu/20465132/Tocopherol_speciation_as_first_screening_for_the_assessment_of_extra_virgin_olive_oil_quality_by_reversed_phase_high_performance_liquid_chromatography_fluorescence_detector","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/20465132/Tocopherol_speciation_as_first_screening_for_the_assessment_of_extra_virgin_olive_oil_quality_by_reversed_phase_high_performance_liquid_chromatography_fluorescence_detector"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="9" data-entity-id="51178964" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/51178964/Assessment_of_olive_oil_adulteration_by_reversed_phase_high_performance_liquid_chromatography_amperometric_detection_of_tocopherols_and_tocotrienols">Assessment of olive oil adulteration by reversed-phase high-performance liquid chromatography/amperometric detection of tocopherols and tocotrienols</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45524342" href="https://independent.academia.edu/FabiolaDionisi">Fabiola Dionisi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of the American Oil Chemists’ Society, 1995</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Assessment of olive oil adulteration by reversed-phase high-performance liquid chromatography/amperometric detection of tocopherols and tocotrienols","attachmentId":69009829,"attachmentType":"pdf","work_url":"https://www.academia.edu/51178964/Assessment_of_olive_oil_adulteration_by_reversed_phase_high_performance_liquid_chromatography_amperometric_detection_of_tocopherols_and_tocotrienols","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/51178964/Assessment_of_olive_oil_adulteration_by_reversed_phase_high_performance_liquid_chromatography_amperometric_detection_of_tocopherols_and_tocotrienols"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="10" data-entity-id="14942665" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/14942665/Methacrylate_ester_based_monolithic_columns_for_nano_LC_separation_of_tocopherols_in_vegetable_oils">Methacrylate ester-based monolithic columns for nano-LC separation of tocopherols in vegetable oils</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="34101217" href="https://independent.academia.edu/ESim%C3%B3alfonso">E. Simó-alfonso</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33933893" href="https://independent.academia.edu/LorenzoCerretani">Lorenzo Cerretani</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Separation Science, 2010</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Methacrylate ester-based monolithic columns for nano-LC separation of tocopherols in vegetable oils","attachmentId":43722738,"attachmentType":"pdf","work_url":"https://www.academia.edu/14942665/Methacrylate_ester_based_monolithic_columns_for_nano_LC_separation_of_tocopherols_in_vegetable_oils","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a 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class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="101889434" href="https://independent.academia.edu/MYRIAMBUSTAMANTERANGEL">MYRIAM BUSTAMANTE RANGEL</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Separation Science, 2009</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Simultaneous extraction of tocotrienols and tocopherols from cereals using pressurized liquid extraction prior to LC determination","attachmentId":70696702,"attachmentType":"pdf","work_url":"https://www.academia.edu/54235652/Simultaneous_extraction_of_tocotrienols_and_tocopherols_from_cereals_using_pressurized_liquid_extraction_prior_to_LC_determination","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span 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