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<!DOCTYPE html> <html lang="en" dir="ltr" prefix="og: https://ogp.me/ns#"> <head> <meta charset="utf-8" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-EH6QSG2FJ8"></script> <script>window.dataLayer = window.dataLayer || [];function gtag(){dataLayer.push(arguments)};gtag("js", new Date());gtag("set", "developer_id.dMDhkMT", true);gtag("config", "G-EH6QSG2FJ8", {"groups":"default","page_placeholder":"PLACEHOLDER_page_location","allow_ad_personalization_signals":false});</script> <meta name="description" content="The Flow Cytometry Core Facility (FCCF), is housed in the Terry Fox Laboratory and provides investigators at the British Columbia Cancer Research Centre access to high quality, cost effective flow cytometry services. By providing these services, as well as the scientific expertise necessary to effectively use this technology, the Facility serves to enhance the scope and quality of scientific research performed at the Research Centre." /> <link rel="canonical" href="https://www.bccrc.ca/dept/tfl/dept/tfl/services/flow-cytometry-core" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <style>.logo a {background-image: url(/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/BCClogo_Research_TerryFoxLaboratory_RGB.png);background-size: contain;}body .region__network .logo a {background-size: contain}</style> <link rel="icon" href="/dept/tfl/dept/tfl/themes/custom/bccrc/favicon.ico" type="image/vnd.microsoft.icon" /> <title>Flow Cytometry Core | Terry Fox Laboratory</title> <link rel="stylesheet" href="//use.typekit.net/rew6qot.css"> <link rel="stylesheet" media="all" href="/dept/tfl/sites/terryfoxlab.ca/files/css/css_AarQcrgLs31lcRjok1l-T7UghOMU01jnxnBu-hVrJTY.css?delta=0&language=en&theme=bccrc&include=eJx1i0EKQyEMRC9k9UxJTKMQNZhI-bcvpYUuSjcz8OYNEm0qogtBbx6X9imJwIIaFATnZLBBNljzUvcx0Pwl-Uw7qN0b1-SXB4_36cF4X3uUT2dWHjwjVw7o6jmWiP7XBruD_O6veAIZDEUU" /> <link rel="stylesheet" media="all" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css" /> <link rel="stylesheet" media="all" href="/dept/tfl/sites/terryfoxlab.ca/files/css/css_b1Imd5Zfo6d9UtggtC_ak93-Ac2c67PnthKR5yWdzt8.css?delta=2&language=en&theme=bccrc&include=eJx1i0EKQyEMRC9k9UxJTKMQNZhI-bcvpYUuSjcz8OYNEm0qogtBbx6X9imJwIIaFATnZLBBNljzUvcx0Pwl-Uw7qN0b1-SXB4_36cF4X3uUT2dWHjwjVw7o6jmWiP7XBruD_O6veAIZDEUU" /> </head> <body class="colour--navy not-front path--node page--service"> <a href="#main-content" class="visually-hidden focusable skip-link"> Skip to main content </a> <div class="dialog-off-canvas-main-canvas" data-off-canvas-main-canvas> <div class="main-container"> <header class="page--service page"> <div class="region__header--inner region--inner"> <section id="block-bccrc-breadcrumbs" class="block block--system-breadcrumb-block block-bccrc-breadcrumbs"> <nav class="breadcrumb" role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/dept/tfl/dept/tfl/">Terry Fox Laboratory</a> </li> <li> <a href="/dept/tfl/dept/tfl/services">Core Facilities</a> </li> <li> Flow Cytometry Core </li> </ol> </nav> </section> <section id="block-bccrc-page-title" class="block block--page-title-block block-bccrc-page-title"> <h1><span>Flow Cytometry Core </span> </h1> </section> </div> </header> <section class="region region__highlight"> <div class="region__highlight--inner region--inner"> <div data-drupal-messages-fallback class="hidden"></div> </div> </section> <main role="main"> <a id="main-content" tabindex="-1"></a> <div class="layout-content"> <section class="region region__content"> <div class="region__content--inner region--inner"> <section id="block-bccrc-content" class="block block--system-main-block block-bccrc-content"> <article class="node node--service full"> <div class="field field--body"><p>The Flow Cytometry Core Facility (FCCF), is housed in the Terry Fox Laboratory and provides investigators at the British Columbia Cancer Research Centre access to high quality, cost effective flow cytometry services. By providing these services, as well as the scientific expertise necessary to effectively use this technology, the Facility serves to enhance the scope and quality of scientific research performed at the Research Centre.</p> <p>Equipment include staff-operated Cell Sorters (16-colour BD FACSAria Fusion & FACSAria III); user-operated cell sorter (8-colour BD FACSMelody)  and cell analyzers (48-colour BD FACSymphony A5 SE spectral analyzer, 28-colour BD FACSymphony A5 and 16-colour BD Fortessa)</p></div> </article> <section class="layout layout__expanding layout__parent__node has-expanding-text paragraph paragraph--type--expanding paragraph--view-mode--default" id="paragraph--209"> <h2 class="section__title" data-expand="field--field_text">Our Equipment</h2><button class="icon" data-expand></button> <div class="field field--field_text"> <p><img src="https://www.bccrc.ca/dept/tfl/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/2019-10/DSC07995%20small.jpg" data-entity-uuid data-entity-type alt="Aria" width="601" height="426" class="align-left" loading="lazy"></p><h3>FACSAria Fusion</h3><p>A biosafety cabinet-enclosed high-speed cell sorting instrument equipped with 375nm, 405nm, 445nm, 488nm, 561nm, and 640nm lasers.</p><p>Detector combination:</p><p>1. FSC, SSC, 2 blue or 445nm, 5 yellow-green, 6 violet, and 3 red.</p><p>2. FSC, SSC, 2 blue or 445nm, 5 yellow-green, 2 UV, and 3 red. </p><h3> </h3><h3>BD FACSAria III</h3><p>A biosafety cabinet-enclosed high-speed cell-sorting instrument equipped with 375nm, 405nm, 488nm, 561nm and 640nm laser.</p><p>Detector combination:</p><ol><li>FSC, SSC, 2 blue, 5 yellow-green, 6 violet, and 3 red.FSC, SSC, 2 blue, 5 yellow-green, 2 UV, and 3 red.</li></ol><h2><img src="https://www.bccrc.ca/dept/tfl/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/2019-10/DSC07968%20small_0.jpg" data-entity-uuid data-entity-type alt="Melody" width="600" height="400" class="align-left" loading="lazy"></h2><h3>BD FACSMelody</h3><p>A BSC-enclosed <strong>user-operated</strong> cell sorting instrument, equipped with 405nm, 488nm and 561nm lasers. Capable of two-way sorting in 8 colours (FSC, SSC, 2 blue, 2 violet, 4 yellow-green) and compatible with multiple tube formats (1.5-, 2.0- and 5.0-mL tubes).</p><p>Uses the new BD FACSChorus software, designed to be intuitive and easy to use so that even a new user can become proficient at sorting quickly. Software guides you through the entire cell sorting process using a streamlined workflow that eliminates manual setup and monitoring.</p><h3>BD FACSymphony A5 SE</h3><p>A high-parameter cell analyzer equipped with <strong>5 lasers</strong> (355nm, 405nm, 488nm, 561nm, 637nm) and a cascade detector array that offers the user a choice between conventional compensation and spectral unmixing across the visible light spectrum, enabling simultaneous analysis of up to<strong> 50 parameters</strong>. This instrument uses the same FACSDiva platform as most equipment in the Flow Core, and our high-power workstations located in the Flow Core can be used for FlowJo analysis of these much larger datasets.</p><h2><img src="https://www.bccrc.ca/dept/tfl/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/2019-10/DSC07956%20small_0.jpg" data-entity-uuid data-entity-type alt="Symphony" width="600" height="400" class="align-left" loading="lazy"></h2><h3>BD FACSymphony A5</h3><p>A custom-order cell analyzer that is equipped with <strong>5 lasers</strong> (355nm, 405nm, 488nm, 561nm, 637nm) and enables the simultaneous measurement of up to <strong>28 colours</strong>. This instrument is equipped with the same FACSDiva software found on most of our equipment and FlowJo Software can be used for high parameter data analysis.</p><h2> </h2><h2><img src="https://www.bccrc.ca/dept/tfl/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/2019-10/DSC08015%20small.jpg" data-entity-uuid data-entity-type alt="Fortessa" width="1000" height="667" class="align-left" loading="lazy"></h2><h3>BD Fortessa I & Fortessa II</h3><p>A multicolour analyzer with 405nm, 488nm, 561nm and 640nm lasers. Detector combination: 2 blue, 5 yellow-green, 6 violet, and 3 red.</p></div> </section> <section class="layout layout__expanding layout__parent__node has-expanding-text paragraph paragraph--type--expanding paragraph--view-mode--default" id="paragraph--126"> <h2 class="section__title" data-expand="field--field_text">Booking an instrument:</h2><button class="icon" data-expand></button> <div class="field field--field_text"> <p>It is mandatory that you sign up for an instrument prior to running your experiment on that instrument. This will guarantee the availability of the instrument for you. If you do NOT book the instrument but another person made that same booking, then that person has the right to the instrument over you during that time slot. </p><p>Please log onto our online schedule at <a href="https://ca.ilab.agilent.com/service_center/show_external/3726">https://ca.ilab.agilent.com/service_center/show_external/3726</a>. If you do NOT have an account, then please click “sign-up” at the top right corner of the page and follow instructions to create an account.</p><p>Please review the following steps for booking our analyzers.</p><p><strong>Analyzers</strong></p><ul><li>Click “Reservation”.</li><li>In order to view the schedule, choose instrument(s) of interest listed in the panel on the right side of the page.</li><li>In the calendar, click the name of the instrument of interest. You will then be re-directed to the calendar of the selected instrument.</li><li>Book your desire time slot by clicking, holding, dragging and letting go of the left-click button of the mouse.</li><li>Fill out further information if necessary.</li><li>Click “save reservation” to confirm your booking.</li></ul><p><strong>Cell-sorters</strong></p><ul><li>Please review the following steps for booking cell-sorting experiments.</li><li>Please log onto our online schedule at <a href="https://ca.ilab.agilent.com/service_center/show_external/3726">https://ca.ilab.agilent.com/service_center/show_external/3726</a>. If you do NOT have an account, then please click “sign-up” and follow instructions to create an account.</li><li>Click “Reservation”</li><li>In order to view the schedule, choose instrument(s) of interest listed in the panel on the right side of the page</li><li>In the calendar, click the name of the instrument of interest. You will then be re-directed to the calendar of the selected instrument.</li><li>Request for your desired time slot by clicking, holding, dragging and letting go of the left-click button of the mouse.</li><li>A request form will appear. Please complete the form entirely, so that the flow core can make approval decisions accordingly.</li></ul><p><strong>Sort request approval process:</strong></p><p>Upon approval of your request, you will receive an automated email informing you of your confirmed time and date of your sort experiment.</p><p>If your sort request is not approved, a flow core staff will contact you by email with further instructions. <br> </p></div> </section> <section class="layout layout__expanding layout__parent__node has-expanding-text paragraph paragraph--type--expanding paragraph--view-mode--default" id="paragraph--124"> <h2 class="section__title" data-expand="field--field_text">Flow Data Storage:</h2><button class="icon" data-expand></button> <div class="field field--field_text"> <p><strong>Cell Sorters</strong><br>Your data (.fcs files) from your sort experiments are transferred to our network drive, which is a temporary storage location.  It is mandatory that you transfer your data from the network drive to your own storage location(s).  Data on our network drive will be deleted 2 weeks after your scheduled experiment.</p><p><strong>Cell Analyzers</strong><br>Please ensure that you export your data to your own storage location.  Upon data transfer, please delete your data from your FACSDiva account.  Feel free to keep data from one or two previous experiments for reference, but do NOT keep more than two experiment worth of data.  Such a practice will significantly reduce the chance of software corruption or crashes.</p></div> </section> <section class="layout layout__expanding layout__parent__node has-expanding-text paragraph paragraph--type--expanding paragraph--view-mode--default" id="paragraph--125"> <h2 class="section__title" data-expand="field--field_text">Cancellation policy:</h2><button class="icon" data-expand></button> <div class="field field--field_text"> <p><strong>Cell Sorters</strong><br>Your booking is confirmed upon the flow core’s approval of your sort request. There are 2 situations regarding cancellation.<br>You can make cancellation requests one business day prior to your scheduled time. You must obtain an email confirmation from the flow core to confirm such a cancellation in order to make such a request effective. You will not be billed.</p><p>You can make cancellation requests less than one business day prior to or on the day of your scheduled sort. This is considered last-minute cancellation. You will not be billed if you can find another person to take your time slot. If not, then the flow core reserves the right to bill you for that time slot. Please email <a href="mailto:flowlab@bccrc.ca">flowlab@bccrc.ca</a>, should you cancel last minute.</p><p><strong>Cell Analyzers</strong><br>Your booking is confirmed immediately after you click “save reservation”. There are 2 situations regarding cancellation.<br>You can cancel your reservation without incurring any cost two hours prior to your scheduled time. You can do so on the online schedule. You will not be billed.</p><p>If you wish to cancel within 2 hours prior to your scheduled time, then this is considered last-minute cancellation. In most cases, you will be billed for this time slot, unless you can find another person to take your time slot. If not, then the flow core reserves the right to bill you for your scheduled time slot. Please email <a href="mailto:flowlab@bccrc.ca">flowlab@bccrc.ca</a>, should you cancel last minute.</p></div> </section> <section class="layout layout__expanding layout__parent__node paragraph paragraph--type--expanding paragraph--view-mode--default" id="paragraph--157"> <h2 class="section__title" >Contact</h2> <div class="field field--field_expanding_summary">For any questions, please send an inquiry using the form below. <br /> <br /> You can also contact us:<br /> Call: 604-675-8145<br /> E-mail: <a href="mailto:flowlab@bccrc.ca">flowlab@bccrc.ca</a><br /> <br /> Flow Cytometry Core Facility<br /> BC Cancer Research Centre<br /> 675 West 10th Avenue, Rm 11-218<br /> Vancouver, BC</div> </section> <section class="layout layout__profiles layout__parent__node paragraph paragraph--type--profiles paragraph--view-mode--default" id="paragraph--272"> <h2 class="section__title">Project Manager</h2> <div class="profiles"><article class="node node--profile teaser"> <figure> <img loading="lazy" src="/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/styles/profile/public/2019-05/BCPCM-portrait.png?itok=nEtgTObd" width="580" height="460" alt="" /> </figure> <section> <h3 class="node__title"> Alynn Shanks, BSc, MLT </h3> <div class="field field--field_profile_title"> <div class="field field--item field--field_profile_title">Research Projects Coordinator</div> </div> <div class="contact"> <div class="field field--field_phone_number"><a href="tel:604-675-8000ext.7738">604-675-8000 ext. 7738</a></div> <a href= "https://www.bccrc.ca/dept/tfl/dept/tfl/contact/182" class="more">Send message</a> </div> </section> </article> </div> </section> <section class="layout layout__profiles layout__parent__node paragraph paragraph--type--profiles paragraph--view-mode--default" id="paragraph--273"> <h2 class="section__title">Staff</h2> <div class="profiles"><article class="node node--profile teaser"> <figure> <img loading="lazy" src="/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/styles/profile/public/2019-05/BCPCM-portrait.png?itok=nEtgTObd" width="580" height="460" alt="" /> </figure> <section> <h3 class="node__title"> Christopher May, PhD </h3> <div class="field field--field_profile_title"> <div class="field field--item field--field_profile_title">POSTDOCTORAL FELLOW</div> </div> <div class="contact"> <a href= "https://www.bccrc.ca/dept/tfl/dept/tfl/contact/860" class="more">Send message</a> </div> </section> </article> <article class="node node--profile teaser"> <figure> <img loading="lazy" src="/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/dept/tfl/sites/terryfoxlab.ca/files/styles/profile/public/2019-05/BCPCM-portrait.png?itok=nEtgTObd" width="580" height="460" alt="" /> </figure> <section> <h3 class="node__title"> Sonja Hummel </h3> <div class="field field--field_profile_title"> <div class="field field--item field--field_profile_title">Research Assistant</div> </div> <div class="contact"> <div class="field field--field_phone_number"><a href="tel:604-675-8145">604-675-8145</a></div> <a href= "https://www.bccrc.ca/dept/tfl/dept/tfl/contact/889" class="more">Send message</a> </div> </section> </article> </div> </section> <section class="layout layout__profiles layout__parent__node paragraph paragraph--type--profiles paragraph--view-mode--default" id="paragraph--274"> </section> <section class="layout layout__profiles 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