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<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE html> <html lang="en" dir="ltr" version="HTML+RDFa+MathML 1.1" xmlns:dc="http://purl.org/dc/terms/" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:og="http://ogp.me/ns#" xmlns:rdfs="http://www.w3.org/2000/01/rdf-schema#" xmlns:sioc="http://rdfs.org/sioc/ns#" xmlns:sioct="http://rdfs.org/sioc/types#" xmlns:skos="http://www.w3.org/2004/02/skos/core#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" xmlns:mml="http://www.w3.org/1998/Math/MathML"> <head profile="http://www.w3.org/1999/xhtml/vocab">  <link rel="dns-prefetch" href="//securepubads.g.doubleclick.net" /> <link rel="dns-prefetch" href="//d1bxh8uas1mnw7.cloudfront.net" /> <link rel="dns-prefetch" href="//scholar.google.com" /> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <link rel="shortcut icon" href="https://gut.bmj.com/sites/default/themes/bmjj/favicon.ico" type="image/vnd.microsoft.icon" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <script>window.dataLayer = window.dataLayer || [];window.dataLayer.push({"page":{"hwTitle":"Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract","hwUrl":"https://gut.bmj.com/content/51/5/628","hwPath":"/content/51/5/628","hwType":"journal page"},"user":{"hwMemberID":"","hwLoggedIntoInstitutionID":"","hwLoggedIntoInstitution":"","hwAccessUsageTerms":"guest","hwRegisteredUser":"no","hwHasFullTextAccess":"no","hwEntitled":"no","mail":"e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855"},"content":{"hwIssueDate":"2002-11-01","hwIssueDateTime":"2002-11-01 00:00:00","hwPublicationDate":"2016-04-18","hwPublicationDateTime":"2016-04-18 12:55:26","hwNlmArticleType":"other","hwDOI":"10.1136/gut.51.5.628","hwAuthors":"G R van den Brink,J C H Hardwick,C Nielsen,C Xu,F J ten Kate,J Glickman,S J H van Deventer,D J Roberts,M P Peppelenbosch","hwContributors":"","hwIsEarlyRelease":"no","hwEissn":"1468-3288","hwPissn":"0017-5749","hwVolume":"51","hwIssue":"5","hwArticleType":"Stomach","hwTabView":"main_tab","hwViewType":"","hwAccessType":"Paywall","hwOpenAccess":"no","hwFreeAccess":"no","hwTaxonomy":"Stomach and duodenum","hwCorpusCode":"gutjnl","hwType":"article","hwSubtitle":null,"hwOverline":null,"hwPisaId":"gutjnl;51/5/628","hwFirstPage":"628","hwLastPage":"633","hwIsLatestVersion":"yes","hwIsCurrentIssue":"no","hwIsOpenIssue":"no","hwHasFullText":"yes","hwHasPDF":"yes","hwParentTitle":"Gut: 51 (5)","hwElectronicPubDate":"2016-04-18","hwElectronicPubDateTime":"2016-04-18 12:55:26"}}); //--><!]]></script> <link rel="canonical" href="https://gut.bmj.com/content/51/5/628" /> <meta name="Generator" content="Drupal 7 (http://drupal.org)" /> <link rel="alternate" type="application/pdf" title="Full Text (PDF)" href="/content/51/5/628.full.pdf" /> <link rel="alternate" type="text/plain" title="Full Text (Plain)" href="/content/51/5/628.full.txt" /> <meta name="issue_cover_image" content="https://gut.bmj.com/sites/default/files/highwire/gutjnl/51/5.cover.gif" /> <meta name="type" content="article" /> <meta name="category" content="other" /> <meta name="HW.identifier" content="/gutjnl/51/5/628.atom" /> <meta name="HW.pisa" content="gutjnl;51/5/628" /> <meta name="DC.Format" content="text/html" /> <meta name="DC.Language" content="en" /> <meta name="DC.Title" content="Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract" /> <meta name="DC.Identifier" content="10.1136/gut.51.5.628" /> <meta name="DC.Date" content="2002-11-01" /> <meta name="DC.Publisher" content="BMJ Publishing Group" /> <meta name="DC.Rights" content="Copyright 2002 by Gut" /> <meta name="DC.AccessRights" content="restricted" /> <meta name="DC.Relation" content="10.1136/gut.51.5.617-a" /> <meta name="DC.Description" content="Background: Sonic hedgehog (Shh) is an important endodermal morphogenetic signal during the development of the vertebrate gut. It controls gastrointestinal patterning in general, and gastric gland formation in particular. We have previously shown that Shh regulates gastric gland proliferation in the adult but detailed analysis of its expression along the adult gastrointestinal tract has never been undertaken. We therefore studied Shh expression along the normal human and rodent adult gastrointestinal tract as well as in intestinal metaplasia of the stomach, gastric and intestinal metaplasia of the oesophagus, and gastric heterotopia in Meckel鈥檚 diverticulum. Methods: The studies were performed with in situ hybridisation and by immunohistochemistry using an antibody that recognises the Shh precursor form. Results: We found that in the normal gastrointestinal tract, high levels of Shh were expressed in the fundic glands of the stomach. Shh expression was also found in fundic gland metaplasia and heterotopia. However, Shh expression was lost in intestinal metaplasia of the stomach. Conclusion: We found a strong correlation between Shh expression and fundic gland differentiation. Our current study therefore provides evidence that in addition to its role in gastric epithelial development, Shh plays a unique role in gastric epithelial differentiation in adults." /> <meta name="DC.Contributor" content="G R van den Brink" /> <meta name="DC.Contributor" content="J C H Hardwick" /> <meta name="DC.Contributor" content="C Nielsen" /> <meta name="DC.Contributor" content="C Xu" /> <meta name="DC.Contributor" content="F J ten Kate" /> <meta name="DC.Contributor" content="J Glickman" /> <meta name="DC.Contributor" content="S J H van Deventer" /> <meta name="DC.Contributor" content="D J Roberts" /> <meta name="DC.Contributor" content="M P Peppelenbosch" /> <meta name="article:published_time" content="2002-11-01" /> <meta name="article:section" content="Stomach" /> <meta name="citation_title" content="Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract" /> <meta name="citation_abstract" lang="en" content="<p><b>Background:</b> Sonic hedgehog (Shh) is an important endodermal morphogenetic signal during the development of the vertebrate gut. It controls gastrointestinal patterning in general, and gastric gland formation in particular. We have previously shown that Shh regulates gastric gland proliferation in the adult but detailed analysis of its expression along the adult gastrointestinal tract has never been undertaken. We therefore studied Shh expression along the normal human and rodent adult gastrointestinal tract as well as in intestinal metaplasia of the stomach, gastric and intestinal metaplasia of the oesophagus, and gastric heterotopia in Meckel鈥檚 diverticulum.</p><p><b>Methods:</b> The studies were performed with in situ hybridisation and by immunohistochemistry using an antibody that recognises the Shh precursor form.</p><p><b>Results:</b> We found that in the normal gastrointestinal tract, high levels of Shh were expressed in the fundic glands of the stomach. Shh expression was also found in fundic gland metaplasia and heterotopia. However, Shh expression was lost in intestinal metaplasia of the stomach.</p><p><b>Conclusion:</b> We found a strong correlation between Shh expression and fundic gland differentiation. Our current study therefore provides evidence that in addition to its role in gastric epithelial development, Shh plays a unique role in gastric epithelial differentiation in adults.</p>" /> <meta name="citation_journal_title" content="Gut" /> <meta name="citation_publisher" content="BMJ Publishing Group" /> <meta name="citation_publication_date" content="2002/11/01" /> <meta name="citation_mjid" content="gutjnl;51/5/628" /> <meta name="citation_id" content="51/5/628" /> <meta name="citation_public_url" content="https://gut.bmj.com/content/51/5/628" /> <meta name="citation_abstract_html_url" content="https://gut.bmj.com/content/51/5/628.abstract" /> <meta name="citation_full_html_url" content="https://gut.bmj.com/content/51/5/628.full" /> <meta name="citation_pdf_url" content="https://gut.bmj.com/content/gutjnl/51/5/628.full.pdf" /> <meta name="citation_issn" content="0017-5749" /> <meta name="citation_issn" content="1468-3288" /> <meta name="citation_doi" content="10.1136/gut.51.5.628" /> <meta name="citation_pmid" content="12377798" /> <meta name="citation_volume" content="51" /> <meta name="citation_issue" content="5" /> <meta name="citation_article_type" content="Other" /> <meta name="citation_section" content="Stomach" /> <meta name="citation_firstpage" content="628" /> <meta name="citation_lastpage" content="633" /> <meta name="citation_author" content="G R van den Brink" /> <meta name="citation_author" content="J C H Hardwick" /> <meta name="citation_author" content="C Nielsen" /> <meta name="citation_author" content="C Xu" /> <meta name="citation_author" content="F J ten Kate" /> <meta name="citation_author" content="J Glickman" /> <meta name="citation_author" content="S J H van Deventer" /> <meta name="citation_author" content="D J Roberts" /> <meta name="citation_author" content="M P Peppelenbosch" /> <meta name="citation_reference" content="citation_title=Cell;citation_journal_abbrev=Cell;citation_title=Morphogenesis.;citation_pages=225-233;citation_volume=96;citation_year=1999;citation_issue=2;citation_pmid=9988217;citation_doi=10.1016/S0092-8674(00)80562-0" /> <meta name="citation_reference" content="citation_title=Nature; 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It controls gastrointestinal patterning in general, and gastric gland formation in particular. We have previously shown that Shh regulates gastric gland proliferation in the adult but detailed analysis of its expression along the adult gastrointestinal tract has never been undertaken. We therefore studied Shh expression along the normal human and rodent adult gastrointestinal tract as well as in intestinal metaplasia of the stomach, gastric and intestinal metaplasia of the oesophagus, and gastric heterotopia in Meckel鈥檚 diverticulum. Methods: The studies were performed with in situ hybridisation and by immunohistochemistry using an antibody that recognises the Shh precursor form. Results: We found that in the normal gastrointestinal tract, high levels of Shh were expressed in the fundic glands of the stomach. Shh expression was also found in fundic gland metaplasia and heterotopia. However, Shh expression was lost in intestinal metaplasia of the stomach. Conclusion: We found a strong correlation between Shh expression and fundic gland differentiation. Our current study therefore provides evidence that in addition to its role in gastric epithelial development, Shh plays a unique role in gastric epithelial differentiation in adults." /> <meta name="og-title" property="og:title" content="Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract" /> <meta name="og-url" property="og:url" content="https://gut.bmj.com/content/51/5/628" /> <meta name="og-site-name" property="og:site_name" content="Gut" /> <meta name="og-description" property="og:description" content="Background: Sonic hedgehog (Shh) is an important endodermal morphogenetic signal during the development of the vertebrate gut. It controls gastrointestinal patterning in general, and gastric gland formation in particular. We have previously shown that Shh regulates gastric gland proliferation in the adult but detailed analysis of its expression along the adult gastrointestinal tract has never been undertaken. We therefore studied Shh expression along the normal human and rodent adult gastrointestinal tract as well as in intestinal metaplasia of the stomach, gastric and intestinal metaplasia of the oesophagus, and gastric heterotopia in Meckel鈥檚 diverticulum. Methods: The studies were performed with in situ hybridisation and by immunohistochemistry using an antibody that recognises the Shh precursor form. Results: We found that in the normal gastrointestinal tract, high levels of Shh were expressed in the fundic glands of the stomach. Shh expression was also found in fundic gland metaplasia and heterotopia. However, Shh expression was lost in intestinal metaplasia of the stomach. Conclusion: We found a strong correlation between Shh expression and fundic gland differentiation. 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class="field-items"><div class="field-item even">Stomach</div></div></div> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-highwire-article-citation col-mobile-10 col-narrow-10 col-normal-11 article-title" > <div class="pane-content"> <div class="highwire-article-citation highwire-citation-type-highwire-article node147905" data-node-nid="147905" id="node-147905--2147171575" data-pisa="gutjnl;51/5/628" data-pisa-master="gutjnl;51/5/628" data-apath="/gutjnl/51/5/628.atom"><cite class="highwire-cite highwire-cite-highwire-article highwire-citation-bmjj-title clearfix"> <div class="highwire-cite-title">Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract</div> <span class="highwire-cite-access"><span class="highwire-citation-access highwire-citation-access-check" data-pisa-id="gutjnl;51/5/628" data-atom-uri="/gutjnl/51/5/628.atom" data-request-view="full"></span></span> </cite> </div> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-highwire-panel-tabs-container col-narrow-12 clear" > <div class="pane-content"> <div data-panels-ajax-tab-preloaded="jnl_template_bmjj_tab_art" id="panels-ajax-tab-container-highwire_article_tabs" class="panels-ajax-tab-container"><div class="panels-ajax-tab-loading" style ="display:none"><img class="loading" src="https://gut.bmj.com/sites/all/modules/contrib/panels_ajax_tab/images/loading.gif" alt="Loading" title="Loading" /></div><div class="panels-ajax-tab-wrap-jnl_template_bmjj_tab_art"><div class="panel-display panel-1col clearfix" > <div class="panel-panel panel-col"> <div><div class="panel-pane pane-highwire-markup author-affiliates col-narrow-12 author-affiliates-corresp article" > <div class="pane-content"> <div class="highwire-markup"><div xmlns="http://www.w3.org/1999/xhtml" class="content-block-markup" xmlns:xhtml="http://www.w3.org/1999/xhtml"><div xmlns:xhtml="http://www.w3.org/1999/xhtml" class="contributors"><ol class="contributor-list" id="contrib-group-1"><li id="contrib-1"><span class="name">G R van den Brink</span><a id="xref-aff-1-1" class="xref-aff" href="#aff-1">1</a>, </li><li id="contrib-2"><span class="name">J C H Hardwick</span><a id="xref-aff-1-2" class="xref-aff" href="#aff-1">1</a>, </li><li id="contrib-3"><span class="name">C Nielsen</span><a id="xref-aff-2-1" class="xref-aff" href="#aff-2">2</a>, </li><li id="contrib-4"><span class="name">C Xu</span><a id="xref-aff-1-3" class="xref-aff" href="#aff-1">1</a>, </li><li id="contrib-5"><span class="name">F J ten Kate</span><a id="xref-aff-3-1" class="xref-aff" href="#aff-3">3</a>, </li><li id="contrib-6"><span class="name">J Glickman</span><a id="xref-aff-4-1" class="xref-aff" href="#aff-4">4</a>, </li><li id="contrib-7"><span class="name">S J H van Deventer</span><a id="xref-aff-5-1" class="xref-aff" href="#aff-5">5</a>, </li><li id="contrib-8"><span class="name">D J Roberts</span><a id="xref-aff-2-2" class="xref-aff" href="#aff-2">2</a>, </li><li id="contrib-9"><span class="name">M P Peppelenbosch</span><a id="xref-aff-1-4" class="xref-aff" href="#aff-1">1</a></li></ol><ol class="affiliation-list"><li class="aff"><a id="aff-1" name="aff-1"></a><address><sup>1</sup>Department of Experimental Internal Medicine, Academic Medical Centre, Amsterdam, the Netherlands </address></li><li class="aff"><a id="aff-2" name="aff-2"></a><address><sup>2</sup>Department of Pediatric Surgery, Massachusetts General Hospital, Brigham and Women鈥檚 Hospital, Boston, USA </address></li><li class="aff"><a id="aff-3" name="aff-3"></a><address><sup>3</sup>Department of Pathology, Academic Medical Centre, Amsterdam, the Netherlands </address></li><li class="aff"><a id="aff-4" name="aff-4"></a><address><sup>4</sup>Department of Pathology, Brigham and Women鈥檚 Hospital, Boston, USA </address></li><li class="aff"><a id="aff-5" name="aff-5"></a><address><sup>5</sup>Department of Gastroenterology, Academic Medical Centre, Amsterdam, the Netherlands</address></li></ol><ol class="corresp-list"><li class="corresp" id="corresp-1"><span class="corresp-label"></span>Correspondence to:<br/> G van den Brink, Lab Exp Internal Medicine, Room G2-130, Academic Medical Centre, Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands;<br/> <span class="em-link"><span class="em-addr">g.r.vandenbrink{at}amc.uva.nl</span></span></li></ol></div></div></div> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-highwire-markup abstract-with-bc" > <div class="pane-content"> <div class="highwire-markup"><div xmlns="http://www.w3.org/1999/xhtml" id="content-block" xmlns:xhtml="http://www.w3.org/1999/xhtml"><div class="article abstract-view"><span class="highwire-journal-article-marker-start"></span><div class="section abstract" id="abstract-1"><h2>Abstract</h2> <p id="p-1"><strong>Background:</strong> Sonic hedgehog (Shh) is an important endodermal morphogenetic signal during the development of the vertebrate gut. It controls gastrointestinal patterning in general, and gastric gland formation in particular. We have previously shown that Shh regulates gastric gland proliferation in the adult but detailed analysis of its expression along the adult gastrointestinal tract has never been undertaken. We therefore studied Shh expression along the normal human and rodent adult gastrointestinal tract as well as in intestinal metaplasia of the stomach, gastric and intestinal metaplasia of the oesophagus, and gastric heterotopia in Meckel鈥檚 diverticulum.</p> <p id="p-2"><strong>Methods:</strong> The studies were performed with in situ hybridisation and by immunohistochemistry using an antibody that recognises the Shh precursor form.</p> <p id="p-3"><strong>Results:</strong> We found that in the normal gastrointestinal tract, high levels of Shh were expressed in the fundic glands of the stomach. Shh expression was also found in fundic gland metaplasia and heterotopia. However, Shh expression was lost in intestinal metaplasia of the stomach.</p> <p id="p-4"><strong>Conclusion:</strong> We found a strong correlation between Shh expression and fundic gland differentiation. Our current study therefore provides evidence that in addition to its role in gastric epithelial development, Shh plays a unique role in gastric epithelial differentiation in adults.</p> </div><ul class="kwd-group KWD"><li class="kwd">sonic hedgehog</li><li class="kwd">fundic gland</li><li class="kwd">fundic gland differentiation</li></ul><ul class="kwd-group ABR"><li class="kwd">Hh, hedgehog</li><li class="kwd">Shh, sonic hedgehog</li><li class="kwd">MUC, mucin</li><li class="kwd">PBS, phosphate buffered saline</li><li class="kwd">strep 尾-gal, streptavidin 尾-galactosidase</li><li class="kwd">HHG, hyperplastic hypersecretory gastropathy</li></ul><span class="highwire-journal-article-marker-end"></span></div><span class="related-urls"></span></div></div> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-dfp-pane oas-ads oas-ads-mid pull-right" > <div class="pane-content"> <div id="dfp-ad-mpu-wrapper" class="dfp-tag-wrapper"> <div id="dfp-ad-mpu" class="dfp-tag-wrapper"> <script type="text/javascript"> googletag.cmd.push(function() { googletag.display("dfp-ad-mpu"); }); </script> </div> </div> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-custom pane-1" > <div class="pane-content"> <p><a href="https://doi.org/10.1136/gut.51.5.628" target="_new">https://doi.org/10.1136/gut.51.5.628</a></p> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-highwire-altmetrics" > <h2 class="pane-title">Statistics from Altmetric.com</h2> <div class="pane-content"> <div data-badge-details="right" data-badge-type="medium-donut" data-doi="10.1136/gut.51.5.628" data-hide-no-mentions="true" class="altmetric-embed"></div> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-bmjj-jumplinks" > <div class="pane-content"> <div class="highwire-list-wrapper"><div class="highwire-list"><ul></ul></div></div> </div> </div> <div class="panel-separator"></div><div class="panel-pane pane-custom pane-2 permissions-box" > <h2 class="pane-title">Request Permissions</h2> <div class="pane-content"> <p>If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. 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