CINXE.COM

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HIF-1 alpha antibody (H1alpha67) [NB100-105]. Western blot image submitted by a verified customer review.","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0019.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/mL of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449].","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0018.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. 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HIF-1 alpha Antibody (H1alpha67) [NB100-105]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-900WB-img0020.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0015.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-900WB-img0017.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Wester blot image submitted by a verified customer review.","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0021.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Western blot image submitted by a verified customer review. HIF-1 alpha Antibody [NB100-449]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0016.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. HIF-1 alpha Antibody (HA111) [NB100-296]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0023.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/lc3-antibody-pack_nb910-40435"],"name":["LC3 Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0013.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license. LC3A Antibody [NB100-2331]","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0009.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0014.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: LC3 Antibody Pack [NB910-40435] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license. LC3A Antibody [NB100-2331]","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Western-Blot-NB910-40435-img0011.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous and extravillous trophoblast. d: decidua; v: villi; DeVe: decidual vessel. Original magnification 40x. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2013/689768/) licensed under a CC-BY license. LC3B Antibody [NB100-2220] ","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0015.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x.","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0008.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review. LC3A Antibody [NB100-2331] ","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0012.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P <\u00a00.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30\u00a0days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10\u00a0\u00b5m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.","url":"https://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-31020241538249.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P <\u00a00.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30\u00a0days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10\u00a0\u00b5m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.","url":"https://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202415284514.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: LC3 Antibody Pack [NB910-40435] - CCI-779 significantly rescued the impaired autophagy-lysosomal pathway in degenerated SGNs of mice. (a) The levels of Ctsb, Ctsd, & Lamp1 & of the autophagic genes Becn1 & Lc3b were significantly higher, & Sqstm1 was lower in the experimental group than in the negative control group, which was determined by quantitative real-time PCR. (b & c) The LAMP1 & CTSD levels determined by western blotting were consistent with the quantitative real-time PCR results. (d & e) Compared with those in the negative control group, the LC3 & BECN1 levels in the experimental groups were significantly increased, as determined by western blot assays. (f & g) The western blot results revealed that the levels of the autophagic cargo receptor SQSTM1 & ubiquitinated proteins were decreased significantly in the experimental group compared with those in the negative control groups. *, the difference between the experimental group & the blank control group was significant (P <\u00a00.05); #, the difference between the experimental group & the negative control group was significant (P <\u00a00.05); CCI-779, experimental group; 30D, negative control group; Con, blank control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals.","url":"https://images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202416163727.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/hif-2-alpha-epas1-antibody-pack_nb100-902hif2"],"name":["HIF-2 alpha/EPAS1 Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]","url":"https://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0010.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]","url":"https://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Western-Blot-NB100-902HIF2-img0012.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Simple Western: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]","url":"https://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Simple-Western-NB100-902HIF2-img0009.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]","url":"https://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Knockout-Validated-NB100-902HIF2-img0013.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe, catalog #410598; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-122) at 1:100 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells. HIF-2 alpha/EPAS1 Antibody [NB100-122]","url":"https://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0014.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Normal ganglia 1 (NG1), normal ganglia 2 (NG2) and IDH-mutant (IDH). Sporadic PGL (Spo. PGL). HIF2 alpha staining. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0127471) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] ","url":"https://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0011.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/53bp1-antibody-pack_nb100-926"],"name":["53BP1 Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Knockout-Validated-NB100-926-img0020.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: 53BP1 Antibody Pack [NB100-926] - Western blot analysis of human 53BP1, blotted with 53BP1 antibody (Catalog #NB100-305). Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa.","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0011.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, 6 or 9 Gy (53BP1 foci green, nuclei blue) using NB100-305. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0152213), licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-926-img0017.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical analysis of human breast tumors stained with 53BP1 antibody (Catalog #NB100-304). Image from verified customer review.","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-NB100-926-img0021.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0023.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: 53BP1 Antibody Pack [NB100-926] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using 53BP1 Antibody (Catalog #NB100-304).","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0009.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Immunohistochemistry-NB100-926-img0012.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - Detection of 53BP1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml 53BP1 Antibody (Catalog #NB100-304) in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0010.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.","url":"https://images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0022.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/hif-1-alpha-antibody-pack_nb100-905"],"name":["HIF-1 alpha Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0048.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. HIF-1 alpha Antibody (H1alpha67) [NB100-105]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-905-img0049.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Chromatin Immunoprecipitation: HIF-1 alpha Antibody Pack [NB100-905] - ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine. HIF-1 alpha Antibody [NB100-479]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-905-img0041.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Simple Western: HIF-1 alpha Antibody Pack [NB100-905] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-905-img0042.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). PDAC with strong (intensity 3) nuclear and moderate (intensity 2) cytoplasmic HIF-1alpha staining (right). Image collected and cropped by CiteAb from the following publication (//wjso.biomedcentral.com/articles/10.1186/s12957-018-1432-4), licensed under a CC-BY license. HIF-1 alpha Antibody (H1alpha67) [NB100-105]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0051.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine. HIF-1 alpha Antibody (H1alpha67) [NB100-105]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Flow-Cytometry-NB100-905-img0050.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0053.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. HIF-1 alpha Antibody (ESEE122) [NB100-131]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0037.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]. ","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0040.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review. HIF-1 alpha Antibody [NB100-449]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0044.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0052.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclea","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0035.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details. HIF-1 alpha Antibody [NB100-479]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0038.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Hif1alpha expression in preimplantation embryos. Co-staining of OCT4 and Hif1alpha in inner cell mass of blastocyst (top) and negative control images without primary antibodies (bottom). Scale bar: 30um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0039.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license. HIF-1 alpha Antibody (ESEE122) [NB100-131]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0045.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review. HIF-1 alpha Antibody (ESEE122) [NB100-131] ","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0047.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]","url":"https://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-905-img0043.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/autophagy-antibody-pack_nb910-94159"],"name":["Autophagy Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0019.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94159] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0020.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG5 was detected using NB110-53818.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0022.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0015.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Simple Western: Autophagy Antibody Pack [NB910-94159] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94159-img0029.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Flow Cytometry: Autophagy Antibody Pack [NB910-94159] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94159-img0027.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0030.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0021.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0028.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - Analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) and mouse ATG5 KO ES cell lysate (- cnt'l) (Lane 2). Atg5-/- ES cells from Dr. Noboru Mizushima [PMID: 11266458] Photo courtesy of Dr. Beth Levine, UT SW Med Center.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0016.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0017.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG16L1. Lane 1: NIH/3T3 Lane 2: HeLa .","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0018.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0024.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0025.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/lc3-antibody-pack_nb910-40752"],"name":["LC3 Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.","url":"https://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0026.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.","url":"https://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-40752-img0017.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: LC3 Antibody Pack [NB910-40752] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220] ","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40752-img0027.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. Stronger co-localization between NeuN and LC3 is apparent at day 1 after SCI. Scale bar is 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license. LC3B Antibody [NB100-2220]","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0029.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Analysis in mouse renal tissue. Image from verifed customer review. LC3A Antibody [NB100-2331]","url":"https://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0030.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.","url":"https://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0025.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.","url":"https://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunohistochemistry-NB910-40752-img0021.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/ape-antibody-pack_nb100-909"],"name":["APE Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.","url":"https://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0013.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).","url":"https://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0014.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.","url":"https://images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0011.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review.","url":"https://images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0012.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101.","url":"https://images.novusbio.com/fullsize/APE1-Antibody-Pack-Immunohistochemistry-NB100-909-img0005.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.","url":"https://images.novusbio.com/fullsize/APE-Antibody-Pack-Simple-Western-NB100-909-img0010.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 (using NB100-116), p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/arnt-hif-1-beta-antibody-pack_nb100-982"],"name":["ARNT/HIF-1 beta Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa.","url":"https://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0010.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of skin, epidermis using ARNT/HIF-1 beta antibody [NB100-110] from the ARNT/HIF-1 beta antibody pack [NB100-982].","url":"https://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0005.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Allele specific HIF-binding at the rs12814794 associated enhancer. B) Allele-specific qPCR for rs12814794 from DNA fragments isolated in HIF-ChIP experiments (HIF-1A and ARNT/HIF-1 beta) or input DNA from primary renal tubular cells (PTC, n = 4 individuals). DNA from individuals homozygous for the AA or GG genotype were used as positive controls. C) Quantification of the two different alleles (A or G) at rs12814794 in the ChIP-seq reads from HIF-1A and ARNT/HIF-1 beta antibody [NB100-110] immunoprecipitations (PTC #1 Fig 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1006872) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-982-img0009.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1a or HIF-2a expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1a-shRNA or HIF-2a-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. ARNT/HIF-1 beta expression was used as a loading control. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0006.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A [NB100-480] and ARNT/HIF-1 beta [NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0007.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of human glioblastoma multi-forme utilizing the ARNT/HIF-1 beta antibody[NB100-124] from the ARNT/HIF-1 beta antibody pack [NB100-982].","url":"https://images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0004.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/survivin-antibody-pack_nb100-911"],"name":["Survivin Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). 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Survivin Antibody (60.11) [NB500-238]","url":"https://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0010.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201].","url":"https://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-911-img0007.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Survivin Antibody Pack [NB100-911] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-NB100-911-img0005.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].","url":"https://images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-911-img0009.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/pluripotent-stem-cell-transcription-factor-antibody-pack_nbp1-42823"],"name":["Pluripotent Stem Cell Transcription Factor Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42823-img0014.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license. OCT4 Antibody [NB100-2379]","url":"https://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-NBP1-42823-img0016.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).","url":"https://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0013.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license. Nanog Antibody [NB100-58842]","url":"https://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0015.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds","url":"https://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0011.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - SOX2 staining (NB110-37235) of human uterus, endometrial glands.","url":"https://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0017.jpg","width":400,"height":400}]]},{"@type":"Thing","url":["https://www.novusbio.com/products/autophagy-antibody-pack_nb910-94877"],"name":["Autophagy Antibody Pack"],"image":[[{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0027.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0028.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG antibody (NB110-53818) used. ","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0031.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0025.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94877-img0037.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94877-img0035.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0038.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0029.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0036.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0040.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0041.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0017.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0032.jpg","width":400,"height":400},{"@type":"ImageObject","caption":"Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine.","url":"https://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0018.jpg","width":400,"height":400}]]}]},{"@context":"http://schema.org","@type":"SiteNavigationElement","about":[{"@type":"Thing","url":"/support/sars-cov-research-resources","name":"COVID-19 Research Tools"},{"@type":"Thing","url":"/productsearch/novus","name":"Antibodies"},{"@type":"Thing","url":"/products/primary-antibodies","name":"Primary Antibodies"},{"@type":"Thing","url":"/conjugatedantibodies.html","name":"Conjugated Antibodies"},{"@type":"Thing","url":"/samplesize","name":"Sample Size 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class="search_facet_name">Loading Control (Simple Western)<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=MAPK%20Family" rel="nofollow"><span data-facet="MAPK Family (1)" class="search_facet_name">MAPK Family<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Mitochondrial%20Markers" rel="nofollow"><span data-facet="Mitochondrial Markers (1)" class="search_facet_name">Mitochondrial Markers<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Mouse%20T%20Cell%20Inhibitory%20Receptor%20(Blocking)" rel="nofollow"><span data-facet="Mouse T Cell Inhibitory Receptor (Blocking) (2)" class="search_facet_name">Mouse T Cell Inhibitory Receptor (Blocking)<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Mre11-Rad50-NBS1%20Complex" rel="nofollow"><span data-facet="Mre11-Rad50-NBS1 Complex (1)" class="search_facet_name">Mre11-Rad50-NBS1 Complex<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=NFkB%20Pathway" rel="nofollow"><span data-facet="NFkB Pathway (1)" class="search_facet_name">NFkB Pathway<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=NK1R" rel="nofollow"><span data-facet="NK1R (1)" class="search_facet_name">NK1R<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Neural%20Stem%20Cell%20Marker" rel="nofollow"><span data-facet="Neural Stem Cell Marker (1)" class="search_facet_name">Neural Stem Cell Marker<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Pancreatic%20Cell%20Marker" rel="nofollow"><span data-facet="Pancreatic Cell Marker (1)" class="search_facet_name">Pancreatic Cell Marker<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Panoptosome" rel="nofollow"><span data-facet="Panoptosome (1)" class="search_facet_name">Panoptosome<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Parthanatos" rel="nofollow"><span data-facet="Parthanatos (1)" class="search_facet_name">Parthanatos<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Pluripotent%20Stem%20Cell%20Transcription%20Factor" rel="nofollow"><span data-facet="Pluripotent Stem Cell Transcription Factor (1)" class="search_facet_name">Pluripotent Stem Cell Transcription Factor<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Pro-apoptotic%20Bcl-2%20Family" rel="nofollow"><span data-facet="Pro-apoptotic Bcl-2 Family (1)" class="search_facet_name">Pro-apoptotic Bcl-2 Family<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=RelA/NFkB%20p65" rel="nofollow"><span data-facet="RelA/NFkB p65 (1)" class="search_facet_name">RelA / NFkB p65<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=SARS%20Nucleocapsid%20Protein" rel="nofollow"><span data-facet="SARS Nucleocapsid Protein (1)" class="search_facet_name">SARS Nucleocapsid Protein<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Src" rel="nofollow"><span data-facet="Src (1)" class="search_facet_name">Src<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Src%20Family%20Kinases" rel="nofollow"><span data-facet="Src Family Kinases (1)" class="search_facet_name">Src Family Kinases<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=Survivin" rel="nofollow"><span data-facet="Survivin (1)" class="search_facet_name">Survivin<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=T%20Cell%20Exhaustion%20Marker%20(Flow)" rel="nofollow"><span data-facet="T Cell Exhaustion Marker (Flow) (1)" class="search_facet_name">T Cell Exhaustion Marker (Flow)<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=T%20Cell%20Exhaustion%20Marker%20(Flow-Directly%20Conjugated)" rel="nofollow"><span data-facet="T Cell Exhaustion Marker (Flow-Directly Conjugated) (1)" class="search_facet_name">T Cell Exhaustion Marker (Flow-Directly Conjugated)<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=TERT" rel="nofollow"><span data-facet="TERT (1)" class="search_facet_name">TERT<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=TLR%20(Cell%20Surface)%20Screening" rel="nofollow"><span data-facet="TLR (Cell Surface) Screening (1)" class="search_facet_name">TLR (Cell Surface) Screening<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=TLR%20(Intracellular)%20Screening" rel="nofollow"><span data-facet="TLR (Intracellular) Screening (1)" class="search_facet_name">TLR (Intracellular) Screening<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=TLR%20Screening" rel="nofollow"><span data-facet="TLR Screening (1)" class="search_facet_name">TLR Screening<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=TLR7" rel="nofollow"><span data-facet="TLR7 (1)" class="search_facet_name">TLR7<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=dsRNA" rel="nofollow"><span data-facet="dsRNA (1)" class="search_facet_name">dsRNA<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=mTOR%20Complex%201" rel="nofollow"><span data-facet="mTOR Complex 1 (1)" class="search_facet_name">mTOR Complex 1<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=mTOR%20Complex%202" rel="nofollow"><span data-facet="mTOR Complex 2 (1)" class="search_facet_name">mTOR Complex 2<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_common_name" class="facet_input"><a href="/product-type/antibody-packs?common_name=mTOR%20Pathway" rel="nofollow"><span data-facet="mTOR Pathway (1)" class="search_facet_name">mTOR Pathway<span class=facet_count> (1)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_applications"><h4><span class="facet_logo facet_logo_open"></span> <span class="facet_name">Applications</span></h4> <div class="facet_list"><input type="text" class="facet_category_search" value="Search"> <div class="search_icon"></div><div class="search_top_category_facets search_category_facets"><ul><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Western%20Blot" rel="nofollow"><span data-facet="Western Blot (68)" class="search_facet_name">Western Blot<span class=facet_count> (68)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Immunohistochemistry" rel="nofollow"><span data-facet="Immunohistochemistry (62)" class="search_facet_name">Immunohistochemistry<span class=facet_count> (62)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Immunocytochemistry/%20Immunofluorescence" rel="nofollow"><span data-facet="Immunocytochemistry/ Immunofluorescence (45)" class="search_facet_name">Immunocytochemistry / Immunofluorescence<span class=facet_count> (45)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Flow%20Cytometry" rel="nofollow"><span data-facet="Flow Cytometry (31)" class="search_facet_name">Flow Cytometry<span class=facet_count> (31)</span></span></a></li><li class="a-z">-------------- All A - Z ---------------</li></ul></div> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Affinity%20Purification" rel="nofollow"><span data-facet="Affinity Purification (1)" class="search_facet_name">Affinity Purification<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Block/Neutralize" rel="nofollow"><span data-facet="Block/Neutralize (2)" class="search_facet_name">Block / Neutralize<span class=facet_count> (2)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Chromatin%20Immunoprecipitation" rel="nofollow"><span data-facet="Chromatin Immunoprecipitation (11)" class="search_facet_name">Chromatin Immunoprecipitation<span class=facet_count> (11)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=CyTOF-ready" rel="nofollow"><span data-facet="CyTOF-ready (6)" class="search_facet_name">CyTOF-ready<span class=facet_count> (6)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Dot%20Blot" rel="nofollow"><span data-facet="Dot Blot (3)" class="search_facet_name">Dot Blot<span class=facet_count> (3)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Dual%20RNAscope%20ISH-IHC" rel="nofollow"><span data-facet="Dual RNAscope ISH-IHC (1)" class="search_facet_name">Dual RNAscope ISH-IHC<span class=facet_count> (1)</span></span></a></li></ul><ul class="more_facets"><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=ELISA" rel="nofollow"><span data-facet="ELISA (7)" class="search_facet_name">ELISA<span class=facet_count> (7)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Flow%20Cytometry" rel="nofollow"><span data-facet="Flow Cytometry (31)" class="search_facet_name">Flow Cytometry<span class=facet_count> (31)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Functional%20Assay" rel="nofollow"><span data-facet="Functional Assay (2)" class="search_facet_name">Functional Assay<span class=facet_count> (2)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Immunocytochemistry/%20Immunofluorescence" rel="nofollow"><span data-facet="Immunocytochemistry / Immunofluorescence (45)" class="search_facet_name">Immunocytochemistry / Immunofluorescence<span class=facet_count> (45)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Immunohistochemistry" rel="nofollow"><span data-facet="Immunohistochemistry (62)" class="search_facet_name">Immunohistochemistry<span class=facet_count> (62)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Immunoprecipitation" rel="nofollow"><span data-facet="Immunoprecipitation (19)" class="search_facet_name">Immunoprecipitation<span class=facet_count> (19)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Knockdown%20Validated" rel="nofollow"><span data-facet="Knockdown Validated (11)" class="search_facet_name">Knockdown Validated<span class=facet_count> (11)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Knockout%20Validated" rel="nofollow"><span data-facet="Knockout Validated (7)" class="search_facet_name">Knockout Validated<span class=facet_count> (7)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=N/A" rel="nofollow"><span data-facet="N/A (1)" class="search_facet_name">N / A<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=SDS-PAGE" rel="nofollow"><span data-facet="SDS-PAGE (1)" class="search_facet_name">SDS-PAGE<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Simple%20Western" rel="nofollow"><span data-facet="Simple Western (20)" class="search_facet_name">Simple Western<span class=facet_count> (20)</span></span></a></li><li><input type="checkbox" name="fc_applications" class="facet_input"><a href="/product-type/antibody-packs?applications=Western%20Blot" rel="nofollow"><span data-facet="Western Blot (68)" class="search_facet_name">Western Blot<span class=facet_count> (68)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_reactivity"><h4><span class="facet_logo facet_logo_open"></span> <span class="facet_name">Reactivity</span></h4> <div class="facet_list"><input type="text" class="facet_category_search" value="Search"> <div class="search_icon"></div><div class="search_top_category_facets search_category_facets"><ul><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Human" rel="nofollow"><span data-facet="Human (64)" class="search_facet_name">Human<span class=facet_count> (64)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Mouse" rel="nofollow"><span data-facet="Mouse (51)" class="search_facet_name">Mouse<span class=facet_count> (51)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Rat" rel="nofollow"><span data-facet="Rat (33)" class="search_facet_name">Rat<span class=facet_count> (33)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Bovine" rel="nofollow"><span data-facet="Bovine (7)" class="search_facet_name">Bovine<span class=facet_count> (7)</span></span></a></li><li class="a-z">-------------- All A - Z ---------------</li></ul></div> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Alligator" rel="nofollow"><span data-facet="Alligator (3)" class="search_facet_name">Alligator<span class=facet_count> (3)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Amphibian" rel="nofollow"><span data-facet="Amphibian (1)" class="search_facet_name">Amphibian<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Avian" rel="nofollow"><span data-facet="Avian (1)" class="search_facet_name">Avian<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Bacteria" rel="nofollow"><span data-facet="Bacteria (3)" class="search_facet_name">Bacteria<span class=facet_count> (3)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Bat" rel="nofollow"><span data-facet="Bat (1)" class="search_facet_name">Bat<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Bovine" rel="nofollow"><span data-facet="Bovine (7)" class="search_facet_name">Bovine<span class=facet_count> (7)</span></span></a></li></ul><ul class="more_facets"><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=C.%20elegans" rel="nofollow"><span data-facet="C. elegans (2)" class="search_facet_name">C. elegans<span class=facet_count> (2)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Canine" rel="nofollow"><span data-facet="Canine (6)" class="search_facet_name">Canine<span class=facet_count> (6)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Chicken" rel="nofollow"><span data-facet="Chicken (1)" class="search_facet_name">Chicken<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Chinese%20Hamster" rel="nofollow"><span data-facet="Chinese Hamster (1)" class="search_facet_name">Chinese Hamster<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Epitope%20Tag" rel="nofollow"><span data-facet="Epitope Tag (1)" class="search_facet_name">Epitope Tag<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Ferret" rel="nofollow"><span data-facet="Ferret (1)" class="search_facet_name">Ferret<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Fish" rel="nofollow"><span data-facet="Fish (2)" class="search_facet_name">Fish<span class=facet_count> (2)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Goat" rel="nofollow"><span data-facet="Goat (1)" class="search_facet_name">Goat<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Golden%20Syrian%20Hamster" rel="nofollow"><span data-facet="Golden Syrian Hamster (1)" class="search_facet_name">Golden Syrian Hamster<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Guinea%20Pig" rel="nofollow"><span data-facet="Guinea Pig (1)" class="search_facet_name">Guinea Pig<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Hamster" rel="nofollow"><span data-facet="Hamster (3)" class="search_facet_name">Hamster<span class=facet_count> (3)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Human" rel="nofollow"><span data-facet="Human (64)" class="search_facet_name">Human<span class=facet_count> (64)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Human%20(Negative)" rel="nofollow"><span data-facet="Human (Negative) (1)" class="search_facet_name">Human (Negative)<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Insect" rel="nofollow"><span data-facet="Insect (1)" class="search_facet_name">Insect<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Invertebrate" rel="nofollow"><span data-facet="Invertebrate (1)" class="search_facet_name">Invertebrate<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Monkey" rel="nofollow"><span data-facet="Monkey (1)" class="search_facet_name">Monkey<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Mouse" rel="nofollow"><span data-facet="Mouse (51)" class="search_facet_name">Mouse<span class=facet_count> (51)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Multi-Species" rel="nofollow"><span data-facet="Multi-Species (1)" class="search_facet_name">Multi-Species<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Naked%20mole-rat" rel="nofollow"><span data-facet="Naked mole-rat (1)" class="search_facet_name">Naked mole-rat<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Plant" rel="nofollow"><span data-facet="Plant (2)" class="search_facet_name">Plant<span class=facet_count> (2)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Porcine" rel="nofollow"><span data-facet="Porcine (4)" class="search_facet_name">Porcine<span class=facet_count> (4)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Primate" rel="nofollow"><span data-facet="Primate (7)" class="search_facet_name">Primate<span class=facet_count> (7)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Rabbit" rel="nofollow"><span data-facet="Rabbit (6)" class="search_facet_name">Rabbit<span class=facet_count> (6)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Rat" rel="nofollow"><span data-facet="Rat (33)" class="search_facet_name">Rat<span class=facet_count> (33)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=SARS-CoV" rel="nofollow"><span data-facet="SARS-CoV (1)" class="search_facet_name">SARS-CoV<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=SARS-CoV-2" rel="nofollow"><span data-facet="SARS-CoV-2 (1)" class="search_facet_name">SARS-CoV-2<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Sheep" rel="nofollow"><span data-facet="Sheep (1)" class="search_facet_name">Sheep<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Virus" rel="nofollow"><span data-facet="Virus (1)" class="search_facet_name">Virus<span class=facet_count> (1)</span></span></a></li><li><input type="checkbox" name="fc_species" class="facet_input"><a href="/product-type/antibody-packs?species=Zebrafish" rel="nofollow"><span data-facet="Zebrafish (2)" class="search_facet_name">Zebrafish<span class=facet_count> (2)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_citation"><h4><span class="facet_logo facet_logo_open"></span> <span class="facet_name">Citation</span></h4> <div class="facet_list"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_count_publications" class="facet_input"><a href="/product-type/antibody-packs?count_publications=1" rel="nofollow"><span data-facet="1 or more (21)" class="search_facet_name">1 or more (21)</span></a></li><li><input type="radio" name="fc_count_publications" class="facet_input"><a href="/product-type/antibody-packs?count_publications=10" rel="nofollow"><span data-facet="10 or more (4)" class="search_facet_name">10 or more (4)</span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_antibody-validation"><h4><span class="facet_logo facet_logo_open"></span> <span class="facet_name">Antibody Validation</span></h4> <div class="facet_list"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="checkbox" name="fc_image_badge_data" class="facet_input"><a href="/product-type/antibody-packs?image_badge_data=Genetic%20Strategies" rel="nofollow"><span data-facet="Genetic Strategies (25)" class="search_facet_name">Genetic Strategies<span class=facet_count> (25)</span></span></a></li><li><input type="checkbox" name="fc_image_badge_data" class="facet_input"><a href="/product-type/antibody-packs?image_badge_data=Biological%20Strategies" rel="nofollow"><span data-facet="Biological Strategies (20)" class="search_facet_name">Biological Strategies<span class=facet_count> (20)</span></span></a></li><li><input type="checkbox" name="fc_image_badge_data" class="facet_input"><a href="/product-type/antibody-packs?image_badge_data=Orthogonal%20Strategies" rel="nofollow"><span data-facet="Orthogonal Strategies (6)" class="search_facet_name">Orthogonal Strategies<span class=facet_count> (6)</span></span></a></li><li><input type="checkbox" name="fc_image_badge_data" class="facet_input"><a href="/product-type/antibody-packs?image_badge_data=Independent%20Antibodies" rel="nofollow"><span data-facet="Independent Antibodies (2)" class="search_facet_name">Independent Antibodies<span class=facet_count> (2)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_reviews"><h4><span class="facet_logo facet_logo_open"></span> <span class="facet_name">Reviews</span></h4> <div class="facet_list"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_stars_novus" class="facet_input"><a href="/product-type/antibody-packs?stars_novus=5.0" rel="nofollow"><span data-facet=" 5 (4)" class="search_facet_name"><span class="searchImage_star5"></span> 5 (4)</span></a></li><li><input type="radio" name="fc_stars_novus" class="facet_input"><a href="/product-type/antibody-packs?stars_novus=4.0" rel="nofollow"><span data-facet=" 4 & Up (5)" class="search_facet_name"><span class="searchImage_star4"></span> 4 & Up (5)</span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_format"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Format</span></h4> <div class="facet_list compacted"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_facet_format" class="facet_input"><a href="/product-type/antibody-packs?facet_format=Sample-size%20Vials" rel="nofollow"><span data-facet="Sample-size Vials (3)" class="search_facet_name">Sample-size Vials<span class=facet_count> (3)</span></span></a></li><li><input type="radio" name="fc_facet_format" class="facet_input"><a href="/product-type/antibody-packs?facet_format=Full-size%20Vials" rel="nofollow"><span data-facet="Full-size Vials (2)" class="search_facet_name">Full-size Vials<span class=facet_count> (2)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_marker"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Marker</span></h4> <div class="facet_list compacted"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_marker" class="facet_input"><a href="/product-type/antibody-packs?marker=T%20cell%20exhaustion" rel="nofollow"><span data-facet="T cell exhaustion (4)" class="search_facet_name">T cell exhaustion<span class=facet_count> (4)</span></span></a></li><li><input type="radio" name="fc_marker" class="facet_input"><a href="/product-type/antibody-packs?marker=Astrocyte%20Marker" rel="nofollow"><span data-facet="Astrocyte Marker (1)" class="search_facet_name">Astrocyte Marker<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_marker" class="facet_input"><a href="/product-type/antibody-packs?marker=Autophagosome%20Marker" rel="nofollow"><span data-facet="Autophagosome Marker (1)" class="search_facet_name">Autophagosome Marker<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_marker" class="facet_input"><a href="/product-type/antibody-packs?marker=Lymphatic%20Vessel%20Marker" rel="nofollow"><span data-facet="Lymphatic Vessel Marker (1)" class="search_facet_name">Lymphatic Vessel Marker<span class=facet_count> (1)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_modification"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Modification</span></h4> <div class="facet_list compacted"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_modification" class="facet_input"><a href="/product-type/antibody-packs?modification=No%20Modification" rel="nofollow"><span data-facet="No Modification (71)" class="search_facet_name">No Modification <span class=facet_count> (71)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_clonality"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Clonality</span></h4> <div class="facet_list compacted"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_clonality" class="facet_input"><a href="/product-type/antibody-packs?clonality=Monoclonal" rel="nofollow"><span data-facet="Monoclonal (6)" class="search_facet_name">Monoclonal<span class=facet_count> (6)</span></span></a></li><li><input type="radio" name="fc_clonality" class="facet_input"><a href="/product-type/antibody-packs?clonality=Polyclonal" rel="nofollow"><span data-facet="Polyclonal (4)" class="search_facet_name">Polyclonal<span class=facet_count> (4)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_gene-symbol"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Gene Symbol</span></h4> <div class="facet_list compacted"><input type="text" class="facet_category_search" value="Search"> <div class="search_icon"></div><div class="search_top_category_facets search_category_facets"><ul><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=BECN1" rel="nofollow"><span data-facet="BECN1 (4)" class="search_facet_name">BECN1<span class=facet_count> (4)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=MAP1LC3B" rel="nofollow"><span data-facet="MAP1LC3B (3)" class="search_facet_name">MAP1LC3B<span class=facet_count> (3)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=HIF1A" rel="nofollow"><span data-facet="HIF1A (2)" class="search_facet_name">HIF1A<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=APEX1" rel="nofollow"><span data-facet="APEX1 (1)" class="search_facet_name">APEX1<span class=facet_count> (1)</span></span></a></li><li class="a-z">-------------- All A - Z ---------------</li></ul></div> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=APEX1" rel="nofollow"><span data-facet="APEX1 (1)" class="search_facet_name">APEX1<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=ARNT" rel="nofollow"><span data-facet="ARNT (1)" class="search_facet_name">ARNT<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=BECN1" rel="nofollow"><span data-facet="BECN1 (4)" class="search_facet_name">BECN1<span class=facet_count> (4)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=BIRC5" rel="nofollow"><span data-facet="BIRC5 (1)" class="search_facet_name">BIRC5<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=BRCA1" rel="nofollow"><span data-facet="BRCA1 (1)" class="search_facet_name">BRCA1<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=EPAS1" rel="nofollow"><span data-facet="EPAS1 (1)" class="search_facet_name">EPAS1<span class=facet_count> (1)</span></span></a></li></ul><ul class="more_facets"><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=ERN1" rel="nofollow"><span data-facet="ERN1 (1)" class="search_facet_name">ERN1<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=HIF1A" rel="nofollow"><span data-facet="HIF1A (2)" class="search_facet_name">HIF1A<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=LAG3" rel="nofollow"><span data-facet="LAG3 (1)" class="search_facet_name">LAG3<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=LRRK2" rel="nofollow"><span data-facet="LRRK2 (1)" class="search_facet_name">LRRK2<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=LYVE1" rel="nofollow"><span data-facet="LYVE1 (1)" class="search_facet_name">LYVE1<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=MAP1LC3B" rel="nofollow"><span data-facet="MAP1LC3B (3)" class="search_facet_name">MAP1LC3B<span class=facet_count> (3)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=MRE11" rel="nofollow"><span data-facet="MRE11 (1)" class="search_facet_name">MRE11<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=N" rel="nofollow"><span data-facet="N (1)" class="search_facet_name">N<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=RELA" rel="nofollow"><span data-facet="RELA (1)" class="search_facet_name">RELA<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=SRC" rel="nofollow"><span data-facet="SRC (1)" class="search_facet_name">SRC<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=TACR1" rel="nofollow"><span data-facet="TACR1 (1)" class="search_facet_name">TACR1<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=TERT" rel="nofollow"><span data-facet="TERT (1)" class="search_facet_name">TERT<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=TLR7" rel="nofollow"><span data-facet="TLR7 (1)" class="search_facet_name">TLR7<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_gene_symbol" class="facet_input"><a href="/product-type/antibody-packs?gene_symbol=TP53BP1" rel="nofollow"><span data-facet="TP53BP1 (1)" class="search_facet_name">TP53BP1<span class=facet_count> (1)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_images-type"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Images Type</span></h4> <div class="facet_list compacted"><input type="text" class="facet_category_search" value="Search"> <div class="search_icon"></div><div class="search_top_category_facets search_category_facets"><ul><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Western%20Blot" rel="nofollow"><span data-facet="Western Blot (61)" class="search_facet_name">Western Blot<span class=facet_count> (61)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunohistochemistry" rel="nofollow"><span data-facet="Immunohistochemistry (29)" class="search_facet_name">Immunohistochemistry<span class=facet_count> (29)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunocytochemistry/%20Immunofluorescence" rel="nofollow"><span data-facet="Immunocytochemistry/ Immunofluorescence (26)" class="search_facet_name">Immunocytochemistry / Immunofluorescence<span class=facet_count> (26)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Flow%20Cytometry" rel="nofollow"><span data-facet="Flow Cytometry (18)" class="search_facet_name">Flow Cytometry<span class=facet_count> (18)</span></span></a></li><li class="a-z">-------------- All A - Z ---------------</li></ul></div> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Chromatin%20Immunoprecipitation" rel="nofollow"><span data-facet="Chromatin Immunoprecipitation (2)" class="search_facet_name">Chromatin Immunoprecipitation<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Chromatin%20Immunoprecipitation%20(ChIP)" rel="nofollow"><span data-facet="Chromatin Immunoprecipitation (ChIP) (1)" class="search_facet_name">Chromatin Immunoprecipitation (ChIP)<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Dot%20Blot" rel="nofollow"><span data-facet="Dot Blot (1)" class="search_facet_name">Dot Blot<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Dual%20RNAscope%20ISH-IHC" rel="nofollow"><span data-facet="Dual RNAscope ISH-IHC (3)" class="search_facet_name">Dual RNAscope ISH-IHC<span class=facet_count> (3)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Flow%20(Intracellular)" rel="nofollow"><span data-facet="Flow (Intracellular) (4)" class="search_facet_name">Flow (Intracellular)<span class=facet_count> (4)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Flow%20Cytometry" rel="nofollow"><span data-facet="Flow Cytometry (18)" class="search_facet_name">Flow Cytometry<span class=facet_count> (18)</span></span></a></li></ul><ul class="more_facets"><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunocytochemistry" rel="nofollow"><span data-facet="Immunocytochemistry (2)" class="search_facet_name">Immunocytochemistry<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunocytochemistry/%20Immunofluorescence" rel="nofollow"><span data-facet="Immunocytochemistry / Immunofluorescence (26)" class="search_facet_name">Immunocytochemistry / Immunofluorescence<span class=facet_count> (26)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunohistochemistry" rel="nofollow"><span data-facet="Immunohistochemistry (29)" class="search_facet_name">Immunohistochemistry<span class=facet_count> (29)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunohistochemistry%20Free-Floating" rel="nofollow"><span data-facet="Immunohistochemistry Free-Floating (1)" class="search_facet_name">Immunohistochemistry Free-Floating<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunohistochemistry-Frozen" rel="nofollow"><span data-facet="Immunohistochemistry-Frozen (2)" class="search_facet_name">Immunohistochemistry-Frozen<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunohistochemistry-Paraffin" rel="nofollow"><span data-facet="Immunohistochemistry-Paraffin (18)" class="search_facet_name">Immunohistochemistry-Paraffin<span class=facet_count> (18)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Immunoprecipitation" rel="nofollow"><span data-facet="Immunoprecipitation (2)" class="search_facet_name">Immunoprecipitation<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Knockout%20Validated" rel="nofollow"><span data-facet="Knockout Validated (4)" class="search_facet_name">Knockout Validated<span class=facet_count> (4)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Live%20Imaging%20%20Microscopy" rel="nofollow"><span data-facet="Live Imaging Microscopy (1)" class="search_facet_name">Live Imaging Microscopy<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=N/A" rel="nofollow"><span data-facet="N/A (4)" class="search_facet_name">N / A<span class=facet_count> (4)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Simple%20Western" rel="nofollow"><span data-facet="Simple Western (17)" class="search_facet_name">Simple Western<span class=facet_count> (17)</span></span></a></li><li><input type="radio" name="fc_images_type" class="facet_input"><a href="/product-type/antibody-packs?images_type=Western%20Blot" rel="nofollow"><span data-facet="Western Blot (61)" class="search_facet_name">Western Blot<span class=facet_count> (61)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_preservative"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Preservative</span></h4> <div class="facet_list compacted"> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_preservative" class="facet_input"><a href="/product-type/antibody-packs?preservative=0.02%%20Sodium%20Azide" rel="nofollow"><span data-facet="0.02% Sodium Azide (2)" class="search_facet_name">0.02% Sodium Azide<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_preservative" class="facet_input"><a href="/product-type/antibody-packs?preservative=0.05%%20Sodium%20Azide" rel="nofollow"><span data-facet="0.05% Sodium Azide (2)" class="search_facet_name">0.05% Sodium Azide<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_preservative" class="facet_input"><a href="/product-type/antibody-packs?preservative=0.09%%20Sodium%20Azide" rel="nofollow"><span data-facet="0.09% Sodium Azide (1)" class="search_facet_name">0.09% Sodium Azide<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_preservative" class="facet_input"><a href="/product-type/antibody-packs?preservative=No%20Preservative" rel="nofollow"><span data-facet="No Preservative (1)" class="search_facet_name">No Preservative<span class=facet_count> (1)</span></span></a></li> </ul> </div> </div> </div><a href="/product-type/antibody-packs?" class="search_clear_link">clear</a><div class="facet_container_research-areas"><h4><span class="facet_logo facet_logo_closed"></span> <span class="facet_name">Research Areas</span></h4> <div class="facet_list compacted"><input type="text" class="facet_category_search" value="Search"> <div class="search_icon"></div><div class="search_top_category_facets search_category_facets"><ul><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Cancer" rel="nofollow"><span data-facet="Cancer (15)" class="search_facet_name">Cancer<span class=facet_count> (15)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Autophagy" rel="nofollow"><span data-facet="Autophagy (8)" class="search_facet_name">Autophagy<span class=facet_count> (8)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=mTOR%20Pathway" rel="nofollow"><span data-facet="mTOR Pathway (7)" class="search_facet_name">mTOR Pathway<span class=facet_count> (7)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Angiogenesis" rel="nofollow"><span data-facet="Angiogenesis (6)" class="search_facet_name">Angiogenesis<span class=facet_count> (6)</span></span></a></li><li class="a-z">-------------- All A - Z ---------------</li></ul></div> <div class="search_all_category_facets search_category_facets"> <ul><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Adaptive%20Immunity" rel="nofollow"><span data-facet="Adaptive Immunity (2)" class="search_facet_name">Adaptive Immunity<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Alzheimers%20Research" rel="nofollow"><span data-facet="Alzheimers Research (1)" class="search_facet_name">Alzheimers Research<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Angiogenesis" rel="nofollow"><span data-facet="Angiogenesis (6)" class="search_facet_name">Angiogenesis<span class=facet_count> (6)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Apoptosis" rel="nofollow"><span data-facet="Apoptosis (6)" class="search_facet_name">Apoptosis<span class=facet_count> (6)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Astrocyte%20Markers" rel="nofollow"><span data-facet="Astrocyte Markers (1)" class="search_facet_name">Astrocyte Markers<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Autophagy" rel="nofollow"><span data-facet="Autophagy (8)" class="search_facet_name">Autophagy<span class=facet_count> (8)</span></span></a></li></ul><ul class="more_facets"><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Base%20Excision%20Repair" rel="nofollow"><span data-facet="Base Excision Repair (1)" class="search_facet_name">Base Excision Repair<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Breast%20Cancer" 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(5)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Cellular%20Markers" rel="nofollow"><span data-facet="Cellular Markers (2)" class="search_facet_name">Cellular Markers<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Checkpoint%20signaling" rel="nofollow"><span data-facet="Checkpoint signaling (1)" class="search_facet_name">Checkpoint signaling<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Chromatin%20Research" rel="nofollow"><span data-facet="Chromatin Research (1)" class="search_facet_name">Chromatin Research<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Core%20ESC%20Like%20Genes" rel="nofollow"><span data-facet="Core ESC Like Genes (1)" class="search_facet_name">Core ESC Like Genes<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Cytokine%20Research" rel="nofollow"><span data-facet="Cytokine Research (1)" class="search_facet_name">Cytokine Research<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=DNA%20Double%20Strand%20Break%20Repair" rel="nofollow"><span data-facet="DNA Double Strand Break Repair (1)" class="search_facet_name">DNA Double Strand Break Repair<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=DNA%20Repair" rel="nofollow"><span data-facet="DNA Repair (3)" class="search_facet_name">DNA Repair<span class=facet_count> (3)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Diabetes%20Research" rel="nofollow"><span data-facet="Diabetes Research (1)" class="search_facet_name">Diabetes Research<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=ER%20Markers" rel="nofollow"><span data-facet="ER Markers (1)" class="search_facet_name">ER Markers<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Endocrinology" rel="nofollow"><span data-facet="Endocrinology (1)" class="search_facet_name">Endocrinology<span class=facet_count> (1)</span></span></a></li><li><input 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rel="nofollow"><span data-facet="GPCR (1)" class="search_facet_name">GPCR<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Gene%20Editing" rel="nofollow"><span data-facet="Gene Editing (1)" class="search_facet_name">Gene Editing<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Gene%20Knockouts" rel="nofollow"><span data-facet="Gene Knockouts (1)" class="search_facet_name">Gene Knockouts<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Gene%20Therapy" rel="nofollow"><span data-facet="Gene Therapy (1)" class="search_facet_name">Gene Therapy<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Growth%20and%20Development" rel="nofollow"><span data-facet="Growth and Development (1)" class="search_facet_name">Growth and Development<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=HIF%20Target%20Genes" rel="nofollow"><span data-facet="HIF Target Genes (2)" class="search_facet_name">HIF Target Genes<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Hypoxia" rel="nofollow"><span data-facet="Hypoxia (5)" class="search_facet_name">Hypoxia<span class=facet_count> (5)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Immune%20System%20Diseases" 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class="search_facet_name">Inflammation<span class=facet_count> (1)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Innate%20Immunity" rel="nofollow"><span data-facet="Innate Immunity (3)" class="search_facet_name">Innate Immunity<span class=facet_count> (3)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Lipid%20and%20Metabolism" rel="nofollow"><span data-facet="Lipid and Metabolism (2)" class="search_facet_name">Lipid and Metabolism<span class=facet_count> (2)</span></span></a></li><li><input type="radio" name="fc_research_areas" class="facet_input"><a href="/product-type/antibody-packs?research_areas=Loading%20Controls" rel="nofollow"><span data-facet="Loading Controls (2)" class="search_facet_name">Loading Controls<span class=facet_count> (2)</span></span></a></li><li><input type="radio" 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Our antibody packs are pre-selected and include a variety of popular targets, so you can save time searching for antibodies. Plus, get more antibodies for less! These cost-effective antibody panels let you test sample sizes before buying full-size antibodies. Find the perfect antibody panel for your research by using the filters to easily sort by target, research application, species reactivity, validation, citations, and more.</p></div><div class="alt_names_toggle">Alternate Names for Antibody Packs</div><div class="alt_names_drop"></div><div class="search_summary">71 Results for ""</div><div class="grpListGrid"> <div class="outdiv"><a href="/product-type/antibody-packs?showGrid=false" class="active_link" rel="nofollow"> <div class="listView"></div> List</a></div> <div class="outdiv listgriddivider"><a href="/product-type/antibody-packs?showGrid=true" class="notactive_link" rel="nofollow"> <div class="gridView"></div> Grid</a></div> </div></div><div id="equalize"><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/hif-1-alpha-antibody-pack_nb100-900wb">NB100-900WB</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB100-900WB" data-name="HIF-1 alpha Antibody Pack" data-position="1" class="ecommerce_link" href="/products/hif-1-alpha-antibody-pack_nb100-900wb" title="HIF-1 alpha Antibody Pack" ><span> HIF-1 alpha Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="36 Publications specific to this product" href="/products/hif-1-alpha-antibody-pack_nb100-900wb#PublicationSection"><span class="publications"></span> (36 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>7 Vials</strong><span class="product_price" data-sku="NB100-900WB"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/hif-1-alpha-antibody-pack_nb100-900wb">NB100-900WB</a> </div><div id="ss_prev0" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-0" data-cycle-prev="#ss_prev0" data-cycle-next="#ss_next0" data-cycle-caption="#image-caption-NB100-900WB" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0019.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. HIF-1 alpha antibody (H1alpha67) [NB100-105]. Western blot image submitted by a verified customer review." class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0019.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. HIF-1 alpha antibody (H1alpha67) [NB100-105]. Western blot image submitted by a verified customer review." alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. HIF-1 alpha antibody (H1alpha67) [NB100-105]. Western blot image submitted by a verified customer review." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0018.jpg" title="<b>Biological Strategies Validation. </b>Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/mL of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]." class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0018.jpg" alt="<b>Biological Strategies Validation. </b>Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/mL of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0022.jpg" title="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-900WB-img0022.jpg" alt="Simple Western: HIF-1 alpha Antibody Pack [NB100-900WB] - Image shows a specific band for HIF-1 alpha in 0.2 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-900WB-img0020.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-900WB-img0020.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0015.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0015.jpg" alt="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-900WB-img0017.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-900WB-img0017.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0021.jpg" title="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Wester blot image submitted by a verified customer review." class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0021.jpg" alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Analysis of HIF-1 alpha in overexpression and endogenous HIF-1 alpha & HIF-2 alpha using anti-HIF-1 alpha antibody NB100-134. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Wester blot image submitted by a verified customer review." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0016.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Western blot image submitted by a verified customer review. HIF-1 alpha Antibody [NB100-449]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0016.jpg" alt="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Western blot image submitted by a verified customer review. HIF-1 alpha Antibody [NB100-449]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0023.jpg" title="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. HIF-1 alpha Antibody (HA111) [NB100-296]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-900WB-img0023.jpg" alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-900WB] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. HIF-1 alpha Antibody (HA111) [NB100-296]" class="big_thumb" /> </a> </div></div><div id="ss_next0" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-0" class="cycle-circle-pager"></div><div id="image-caption-NB100-900WB"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Canine">Ca</span>, <span title="Primate">Pm</span>, <span title="Rabbit">Rb</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Canine">Ca</span>, <span title="Primate">Pm</span>, <span title="Rabbit">Rb</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span></div><div class="field not3column"><label>Host:</label><div class="value">Mouse</div></div><div class="forcol3 bottom_margin"><strong>Host:</strong> Mouse</div><div class="field not3column"><label>Validated by:</label><div class="value">  <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>   <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB100-900WB","Product Name":"HIF-1 alpha Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Canine, Primate, Rabbit","Applications":"WB, Simple Western, ICC\/IF, IHC, IP, ChIP","Source":"","Purification":"","Immunogen":"For NB100-105, NB100-134: Fusion protein containing amino acids 432-528 of human HIF-1 alpha. For NB100-296: Fusion protein containing amino acids 329-530 of human HIF-1 alpha. >br\/>For NB100-449: A synthetic peptide mapping to a region between residues 775 and the C-terminus.For NB100-47<span class=\"compare_read_more_click\">...(read more)<\/span><span class=\"compare_read_more\">9SS: A fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221].<\/span>","Citations":"36","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/hif-1-alpha-antibody-pack_nb100-900wb\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/lc3-antibody-pack_nb910-40435">NB910-40435</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB910-40435" data-name="LC3 Antibody Pack" data-position="2" class="ecommerce_link" href="/products/lc3-antibody-pack_nb910-40435" title="LC3 Antibody Pack" ><span> LC3 Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="3 Reviews for this product" href="/products/lc3-antibody-pack_nb910-40435#ReviewsSection"><span class="stars5"></span> (3 Reviews)</a> </div> <div class="segment"> <a title="14 Publications specific to this product" href="/products/lc3-antibody-pack_nb910-40435#PublicationSection"><span class="publications"></span> (14 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>1 Pack</strong><span class="product_price" data-sku="NB910-40435-1Pack"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/lc3-antibody-pack_nb910-40435">NB910-40435</a> </div><div id="ss_prev1" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-1" data-cycle-prev="#ss_prev1" data-cycle-next="#ss_next1" data-cycle-caption="#image-caption-NB910-40435" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0013.jpg" title="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]" class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0013.jpg" title="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]" alt="Western Blot: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0009.jpg" title="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license. LC3A Antibody [NB100-2331]" class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0009.jpg" alt="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license. LC3A Antibody [NB100-2331]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0014.jpg" title="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0014.jpg" alt="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Western-Blot-NB910-40435-img0011.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40435] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license. LC3A Antibody [NB100-2331]" class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Western-Blot-NB910-40435-img0011.jpg" alt="<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40435] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license. LC3A Antibody [NB100-2331]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0015.jpg" title="Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous and extravillous trophoblast. d: decidua; v: villi; DeVe: decidual vessel. Original magnification 40x. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2013/689768/) licensed under a CC-BY license. LC3B Antibody [NB100-2220] " class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0015.jpg" alt="Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous and extravillous trophoblast. d: decidua; v: villi; DeVe: decidual vessel. Original magnification 40x. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2013/689768/) licensed under a CC-BY license. LC3B Antibody [NB100-2220] " class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0008.jpg" title="Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x." class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0008.jpg" alt="Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0012.jpg" title="Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review. LC3A Antibody [NB100-2331] " class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0012.jpg" alt="Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review. LC3A Antibody [NB100-2331] " class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-31020241538249.jpg" title="Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P <聽0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30聽days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10聽碌m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_lightbox"> <img src="//images.novusbio.com/images/nb910-40435_lc3-antibody-pack-31020241538249.jpg" alt="Immunocytochemistry/ Immunofluorescence: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P <聽0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30聽days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10聽碌m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202415284514.jpg" title="Western Blot: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P <聽0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30聽days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10聽碌m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_lightbox"> <img src="//images.novusbio.com/images/nb910-40435_lc3-antibody-pack-310202415284514.jpg" alt="Western Blot: LC3 Antibody Pack [NB910-40435] - The autophagy level was increased in degenerated mouse SGNs. (a-c) Western blot results revealed that the levels of the autophagy-related proteins LC3 & BECN1 were increased in the degenerated SGNs on the 5th, 15th & 30th day after ototoxic drug administration & were significantly different from those in the normal mice. *, P <聽0.05. (d) Immunofluorescence staining of LC3 puncta (red) also demonstrated that the LC3 level in the degenerated SGNs (green) was significantly increased on the 30th day after drug administration. Con, normal mice without drug treatment; 30D, 30聽days after drug administration. Images of immunofluorescence staining were taken from the middle turn of cochlea. Scale bar: 10聽碌m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/nb910-40435_lc3-antibody-pack-310202416163727.jpg" title="Western Blot: LC3 Antibody Pack [NB910-40435] - CCI-779 significantly rescued the impaired autophagy-lysosomal pathway in degenerated SGNs of mice. (a) The levels of Ctsb, Ctsd, & Lamp1 & of the autophagic genes Becn1 & Lc3b were significantly higher, & Sqstm1 was lower in the experimental group than in the negative control group, which was determined by quantitative real-time PCR. (b & c) The LAMP1 & CTSD levels determined by western blotting were consistent with the quantitative real-time PCR results. (d & e) Compared with those in the negative control group, the LC3 & BECN1 levels in the experimental groups were significantly increased, as determined by western blot assays. (f & g) The western blot results revealed that the levels of the autophagic cargo receptor SQSTM1 & ubiquitinated proteins were decreased significantly in the experimental group compared with those in the negative control groups. *, the difference between the experimental group & the blank control group was significant (P <聽0.05); #, the difference between the experimental group & the negative control group was significant (P <聽0.05); CCI-779, experimental group; 30D, negative control group; Con, blank control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_lightbox"> <img src="//images.novusbio.com/images/nb910-40435_lc3-antibody-pack-310202416163727.jpg" alt="Western Blot: LC3 Antibody Pack [NB910-40435] - CCI-779 significantly rescued the impaired autophagy-lysosomal pathway in degenerated SGNs of mice. (a) The levels of Ctsb, Ctsd, & Lamp1 & of the autophagic genes Becn1 & Lc3b were significantly higher, & Sqstm1 was lower in the experimental group than in the negative control group, which was determined by quantitative real-time PCR. (b & c) The LAMP1 & CTSD levels determined by western blotting were consistent with the quantitative real-time PCR results. (d & e) Compared with those in the negative control group, the LC3 & BECN1 levels in the experimental groups were significantly increased, as determined by western blot assays. (f & g) The western blot results revealed that the levels of the autophagic cargo receptor SQSTM1 & ubiquitinated proteins were decreased significantly in the experimental group compared with those in the negative control groups. *, the difference between the experimental group & the blank control group was significant (P <聽0.05); #, the difference between the experimental group & the negative control group was significant (P <聽0.05); CCI-779, experimental group; 30D, negative control group; Con, blank control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30706760), licensed under a CC-BY license. Not internally tested by Novus Biologicals." class="big_thumb" /> </a> </div></div><div id="ss_next1" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-1" class="cycle-circle-pager"></div><div id="image-caption-NB910-40435"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Alligator">Al</span>, <span title="Amphibian">Am</span>, <span title="Avian">Av</span>, <span title="Bacteria">Ba</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Chicken">Ch</span>, <span title="Chinese Hamster">ChHa</span>, <span title="Fish">Fi</span>, <span title="Guinea Pig">Gp</span>, <span title="Hamster">Ha</span>, <span title="Invertebrate">In</span>, <span title="Plant">Pl</span>, <span title="Primate">Pm</span>, <span title="Monkey">Pm</span>, <span title="Rabbit">Rb</span>, <span title="Golden Syrian Hamster">SyHa</span>, <span title="Zebrafish">Ze</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Alligator">Al</span>, <span title="Amphibian">Am</span>, <span title="Avian">Av</span>, <span title="Bacteria">Ba</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Chicken">Ch</span>, <span title="Chinese Hamster">ChHa</span>, <span title="Fish">Fi</span>, <span title="Guinea Pig">Gp</span>, <span title="Hamster">Ha</span>, <span title="Invertebrate">In</span>, <span title="Plant">Pl</span>, <span title="Primate">Pm</span>, <span title="Monkey">Pm</span>, <span title="Rabbit">Rb</span>, <span title="Golden Syrian Hamster">SyHa</span>, <span title="Zebrafish">Ze</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Functional Assay">Func</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="N/A">NULL</span>, <span class="application-tooltip-text" title="SDS-PAGE">PAGE</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span>, <span class="application-tooltip-text" title="Knockout Validated">KO</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Functional Assay">Func</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="N/A">NULL</span>, <span class="application-tooltip-text" title="SDS-PAGE">PAGE</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span>, <span class="application-tooltip-text" title="Knockout Validated">KO</span></div><div class="field not3column"><label>Validated by:</label><div class="value">  <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>   <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB910-40435","Product Name":"LC3 Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Porcine, Alligator, Amphibian, Avian, Bacteria, Bovine, Canine, Chicken, Chinese Hamster, Fish, Guinea Pig, Hamster, Invertebrate, Plant, Primate, Monkey, Rabbit, Golden Syrian Hamster, Zebrafish","Applications":"WB, Simple Western, ELISA, Flow, Func, ICC\/IF, IHC, IP, NULL, PAGE, WB, ChIP, IHC, KD, KO","Source":"","Purification":"","Immunogen":"For NB100-2331: A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121) [Uniprot: Q9H492].For NB100-2220: Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues<span class=\"compare_read_more_click\">...(read more)<\/span><span class=\"compare_read_more\"> 1-100) [UniProt# Q9GZQ8].<\/span>","Citations":"14","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/lc3-antibody-pack_nb910-40435\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/hif-2-alpha-epas1-antibody-pack_nb100-902hif2">NB100-902HIF2</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB100-902HIF2" data-name="HIF-2 alpha/EPAS1 Antibody Pack" data-position="3" class="ecommerce_link" href="/products/hif-2-alpha-epas1-antibody-pack_nb100-902hif2" title="HIF-2 alpha/EPAS1 Antibody Pack" ><span> HIF-2 alpha/EPAS1 Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="1 Review for this product" href="/products/hif-2-alpha-epas1-antibody-pack_nb100-902hif2#ReviewsSection"><span class="stars4"></span> (1 Review)</a> </div> <div class="segment"> <a title="12 Publications specific to this product" href="/products/hif-2-alpha-epas1-antibody-pack_nb100-902hif2#PublicationSection"><span class="publications"></span> (12 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>4 Vials</strong><span class="product_price" data-sku="NB100-902HIF2"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/hif-2-alpha-epas1-antibody-pack_nb100-902hif2">NB100-902HIF2</a> </div><div id="ss_prev2" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-2" data-cycle-prev="#ss_prev2" data-cycle-next="#ss_next2" data-cycle-caption="#image-caption-NB100-902HIF2" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0010.jpg" title="Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0010.jpg" title="Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]" alt="Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Analysis of HIF-2 alpha in human endometrium using. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Western-Blot-NB100-902HIF2-img0012.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-2-alpha-EPAS1-Antibody-Pack-Western-Blot-NB100-902HIF2-img0012.jpg" alt="<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - WNT11 is induced by hypoxia or hypoxic mimetics in different cell types. Immunoblot analyses of HeLa cells under normal air or hypoxia for 24 hrs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Simple-Western-NB100-902HIF2-img0009.jpg" title="Simple Western: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-2-alpha-EPAS1-Antibody-Pack-Simple-Western-NB100-902HIF2-img0009.jpg" alt="Simple Western: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Knockout-Validated-NB100-902HIF2-img0013.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-2-alpha-EPAS1-Antibody-Pack-Knockout-Validated-NB100-902HIF2-img0013.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - HIF-1alpha is the predominant transcriptional regulator of WNT11 expression during hypoxia. EMSCs isolated from the indicated mouse genotypes were infected with lentivirus expressing GFP or Cre recombinase. Non-infected cells and GFP infected cells served as controls. Immunoblot analyses of EMSCs derived from the indicated genotypes treated with 0.1 mM DMOG for 24 hrs. Attenuated WNT11 expression in Hif-1alpha KO EMSCs (lenti-Cre infected Hif-1af/f). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep21520) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody [NB100-122]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0014.jpg" title="<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe, catalog #410598; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-122) at 1:100 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells. HIF-2 alpha/EPAS1 Antibody [NB100-122]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0014.jpg" alt="<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe, catalog #410598; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog # NB100-122) at 1:100 dilution with one-hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells. HIF-2 alpha/EPAS1 Antibody [NB100-122]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0011.jpg" title="Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Normal ganglia 1 (NG1), normal ganglia 2 (NG2) and IDH-mutant (IDH). Sporadic PGL (Spo. PGL). HIF2 alpha staining. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0127471) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] " class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-2-alpha-EPAS1-Antibody-Pack-Immunohistochemistry-NB100-902HIF2-img0011.jpg" alt="Immunohistochemistry: HIF-2 alpha/EPAS1 Antibody Pack [NB100-902HIF2] - Normal ganglia 1 (NG1), normal ganglia 2 (NG2) and IDH-mutant (IDH). Sporadic PGL (Spo. PGL). HIF2 alpha staining. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0127471) licensed under a CC-BY license. HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] " class="big_thumb" /> </a> </div></div><div id="ss_next2" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-2" class="cycle-circle-pager"></div><div id="image-caption-NB100-902HIF2"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Hamster">Ha</span>, <span title="Rabbit">Rb</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Hamster">Ha</span>, <span title="Rabbit">Rb</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span></div><div class="field not3column"><label>Host:</label><div class="value">Mouse</div></div><div class="forcol3 bottom_margin"><strong>Host:</strong> Mouse</div><div class="field not3column"><label>Validated by:</label><div class="value">  <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasOrthogonal" title="Orthogonal Strategies"> </a></span>  </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>   <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasOrthogonal" title="Orthogonal Strategies"> </a></span>  </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB100-902HIF2","Product Name":"HIF-2 alpha\/EPAS1 Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Hamster, Rabbit","Applications":"WB, Simple Western, ELISA, Flow, ICC\/IF, IHC, IP, ChIP, KD","Source":"","Purification":"","Immunogen":"For NB100-122: Proprietary peptide derived from the mouse\/human HIF-2a. For NB100-132: Human HIF-2a amino acids 535-631.","Citations":"12","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/hif-2-alpha-epas1-antibody-pack_nb100-902hif2\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/53bp1-antibody-pack_nb100-926">NB100-926</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB100-926" data-name="53BP1 Antibody Pack" data-position="4" class="ecommerce_link" href="/products/53bp1-antibody-pack_nb100-926" title="53BP1 Antibody Pack" ><span> 53BP1 Antibody Pack</span></a><div class="ibubble not3column" title="</span><b>Purity:</b> Immunogen affinity purified<br/><b>Immunogen:</b> See individual datasheets.<br/><b>Other Names:</b> 53BP1, p202, p53-binding protein 1, p53bp1, TDRD30, TP53-Binding Protein 1, tumor protein 53-binding protein, 1, tumor protein p53 binding protein 1, tumor protein p53-binding protein, 1, tumor suppressor p53-binding protein 1<br/>"></div></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="11 Publications specific to this product" href="/products/53bp1-antibody-pack_nb100-926#PublicationSection"><span class="publications"></span> (11 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>1 Pack</strong><span class="product_price" data-sku="NB100-926-1Pack"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/53bp1-antibody-pack_nb100-926">NB100-926</a> </div><div id="ss_prev3" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-3" data-cycle-prev="#ss_prev3" data-cycle-next="#ss_next3" data-cycle-caption="#image-caption-NB100-926" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Knockout-Validated-NB100-926-img0020.jpg" title="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Knockout-Validated-NB100-926-img0020.jpg" title="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei." alt="Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0011.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody Pack [NB100-926] - Western blot analysis of human 53BP1, blotted with 53BP1 antibody (Catalog #NB100-305). Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0011.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: 53BP1 Antibody Pack [NB100-926] - Western blot analysis of human 53BP1, blotted with 53BP1 antibody (Catalog #NB100-305). Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-926-img0017.jpg" title="Immunocytochemistry/Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, 6 or 9 Gy (53BP1 foci green, nuclei blue) using NB100-305. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0152213), licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-926-img0017.jpg" alt="Immunocytochemistry/Immunofluorescence: 53BP1 Antibody Pack [NB100-926] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, 6 or 9 Gy (53BP1 foci green, nuclei blue) using NB100-305. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0152213), licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-NB100-926-img0021.jpg" title="Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical analysis of human breast tumors stained with 53BP1 antibody (Catalog #NB100-304). Image from verified customer review." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-NB100-926-img0021.jpg" alt="Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical analysis of human breast tumors stained with 53BP1 antibody (Catalog #NB100-304). Image from verified customer review." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0023.jpg" title="Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0023.jpg" alt="Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0009.jpg" title="Western Blot: 53BP1 Antibody Pack [NB100-926] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using 53BP1 Antibody (Catalog #NB100-304)." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Western-Blot-NB100-926-img0009.jpg" alt="Western Blot: 53BP1 Antibody Pack [NB100-926] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using 53BP1 Antibody (Catalog #NB100-304)." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Immunohistochemistry-NB100-926-img0012.jpg" title="Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Immunohistochemistry-NB100-926-img0012.jpg" alt="Immunohistochemistry: 53BP1 Antibody Pack [NB100-926] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0010.jpg" title="Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - Detection of 53BP1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml 53BP1 Antibody (Catalog #NB100-304) in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red)." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0010.jpg" alt="Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - Detection of 53BP1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml 53BP1 Antibody (Catalog #NB100-304) in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red)." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0022.jpg" title="Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647." class="big_lightbox"> <img src="//images.novusbio.com/images/53BP1-Antibody-Pack-Flow-Cytometry-NB100-926-img0022.jpg" alt="Flow Cytometry: 53BP1 Antibody Pack [NB100-926] - An intracellular stain was performed on RH-30 cells with 53BP1 Antibody (Catalog #NB100-304AF647) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647." class="big_thumb" /> </a> </div></div><div id="ss_next3" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-3" class="cycle-circle-pager"></div><div id="image-caption-NB100-926"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Bat">Bt</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Fish">Fi</span>, <span title="Goat">Gt</span>, <span title="Naked mole-rat">Nm</span>, <span title="Primate">Pm</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Bat">Bt</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Fish">Fi</span>, <span title="Goat">Gt</span>, <span title="Naked mole-rat">Nm</span>, <span title="Primate">Pm</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Functional Assay">Func</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span>, <span class="application-tooltip-text" title="Knockout Validated">KO</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Functional Assay">Func</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span>, <span class="application-tooltip-text" title="Knockout Validated">KO</span></div><div class="field not3column"><label>Host:</label><div class="value">Rabbit</div></div><div class="forcol3 bottom_margin"><strong>Host:</strong> Rabbit</div><div class="field not3column"><label>Validated by:</label><div class="value">    <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>     <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB100-926","Product Name":"53BP1 Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Bat, Bovine, Canine, Fish, Goat, Naked mole-rat, Primate","Applications":"WB, Flow, Func, ICC\/IF, IHC, IP, WB, ChIP, KD, KO","Source":"","Purification":"","Immunogen":"See individual datasheets.","Citations":"11","Purity":"Immunogen affinity purified","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/53bp1-antibody-pack_nb100-926\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/hif-1-alpha-antibody-pack_nb100-905">NB100-905</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB100-905" data-name="HIF-1 alpha Antibody Pack" data-position="5" class="ecommerce_link" href="/products/hif-1-alpha-antibody-pack_nb100-905" title="HIF-1 alpha Antibody Pack" ><span> HIF-1 alpha Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="2 Reviews for this product" href="/products/hif-1-alpha-antibody-pack_nb100-905#ReviewsSection"><span class="stars5"></span> (2 Reviews)</a> </div> <div class="segment"> <a title="4 Publications specific to this product" href="/products/hif-1-alpha-antibody-pack_nb100-905#PublicationSection"><span class="publications"></span> (4 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>7 Vials</strong><span class="product_price" data-sku="NB100-905"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/hif-1-alpha-antibody-pack_nb100-905">NB100-905</a> </div><div id="ss_prev4" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-4" data-cycle-prev="#ss_prev4" data-cycle-next="#ss_next4" data-cycle-caption="#image-caption-NB100-905" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0048.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0048.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" alt="Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-905-img0049.jpg" title="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-905-img0049.jpg" alt="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-905-img0041.jpg" title="Chromatin Immunoprecipitation: HIF-1 alpha Antibody Pack [NB100-905] - ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine. HIF-1 alpha Antibody [NB100-479]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-905-img0041.jpg" alt="Chromatin Immunoprecipitation: HIF-1 alpha Antibody Pack [NB100-905] - ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine. HIF-1 alpha Antibody [NB100-479]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-905-img0042.jpg" title="Simple Western: HIF-1 alpha Antibody Pack [NB100-905] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-905-img0042.jpg" alt="Simple Western: HIF-1 alpha Antibody Pack [NB100-905] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0051.jpg" title="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). PDAC with strong (intensity 3) nuclear and moderate (intensity 2) cytoplasmic HIF-1alpha staining (right). Image collected and cropped by CiteAb from the following publication (//wjso.biomedcentral.com/articles/10.1186/s12957-018-1432-4), licensed under a CC-BY license. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0051.jpg" alt="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). PDAC with strong (intensity 3) nuclear and moderate (intensity 2) cytoplasmic HIF-1alpha staining (right). Image collected and cropped by CiteAb from the following publication (//wjso.biomedcentral.com/articles/10.1186/s12957-018-1432-4), licensed under a CC-BY license. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Flow-Cytometry-NB100-905-img0050.jpg" title="Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Flow-Cytometry-NB100-905-img0050.jpg" alt="Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine. HIF-1 alpha Antibody (H1alpha67) [NB100-105]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0053.jpg" title="<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0053.jpg" alt="<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0037.jpg" title="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. HIF-1 alpha Antibody (ESEE122) [NB100-131]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0037.jpg" alt="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. HIF-1 alpha Antibody (ESEE122) [NB100-131]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0040.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]. " class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0040.jpg" alt="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]. " class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0044.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review. HIF-1 alpha Antibody [NB100-449]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0044.jpg" alt="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review. HIF-1 alpha Antibody [NB100-449]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0052.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0052.jpg" alt="<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0035.jpg" title="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclea" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0035.jpg" alt="Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclea" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0038.jpg" title="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details. HIF-1 alpha Antibody [NB100-479]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0038.jpg" alt="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details. HIF-1 alpha Antibody [NB100-479]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0039.jpg" title="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Hif1alpha expression in preimplantation embryos. Co-staining of OCT4 and Hif1alpha in inner cell mass of blastocyst (top) and negative control images without primary antibodies (bottom). Scale bar: 30um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0039.jpg" alt="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Hif1alpha expression in preimplantation embryos. Co-staining of OCT4 and Hif1alpha in inner cell mass of blastocyst (top) and negative control images without primary antibodies (bottom). Scale bar: 30um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0045.jpg" title="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license. HIF-1 alpha Antibody (ESEE122) [NB100-131]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0045.jpg" alt="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license. HIF-1 alpha Antibody (ESEE122) [NB100-131]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0047.jpg" title="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review. HIF-1 alpha Antibody (ESEE122) [NB100-131] " class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0047.jpg" alt="Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review. HIF-1 alpha Antibody (ESEE122) [NB100-131] " class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-905-img0043.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]" class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-905-img0043.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]" class="big_thumb" /> </a> </div></div><div id="ss_next4" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-4" class="cycle-circle-pager"></div><div id="image-caption-NB100-905"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Canine">Ca</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Canine">Ca</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span></div><div class="field not3column"><label>Sizes:</label><div class="value">Sample Size Available</div></div><div class="forcol3 bottom_margin"><strong>Sizes:</strong> Sample Size Available</div><div class="field not3column"><label>Validated by:</label><div class="value">  <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasOrthogonal" title="Orthogonal Strategies"> </a></span>  </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>   <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasOrthogonal" title="Orthogonal Strategies"> </a></span>  </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB100-905","Product Name":"HIF-1 alpha Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Canine","Applications":"WB, Simple Western, Flow, ICC\/IF, IHC, IP","Source":"","Purification":"","Immunogen":"For NB 100-654: Human HIF-1 alpha amino acids 432-528. For NB 100-105: Human HIF-1 alpha amino acids 432-528. For NB 100-131: Human HIF-1 alpha amino acids 329-530. For NB 100-449: Human HIF-1 alpha amino acids 775-826. For NB 100-479: Antibody was developed against a fusion protein including<span class=\"compare_read_more_click\">...(read more)<\/span><span class=\"compare_read_more\"> amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221].<\/span>","Citations":"4","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/hif-1-alpha-antibody-pack_nb100-905\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/autophagy-antibody-pack_nb910-94159">NB910-94159</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB910-94159" data-name="Autophagy Antibody Pack" data-position="6" class="ecommerce_link" href="/products/autophagy-antibody-pack_nb910-94159" title="Autophagy Antibody Pack" ><span> Autophagy Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="1 Review for this product" href="/products/autophagy-antibody-pack_nb910-94159#ReviewsSection"><span class="stars5"></span> (1 Review)</a> </div> <div class="segment" style="float:right;"> <strong>6 Vials</strong><span class="product_price" data-sku="NB910-94159"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/autophagy-antibody-pack_nb910-94159">NB910-94159</a> </div><div id="ss_prev5" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-5" data-cycle-prev="#ss_prev5" data-cycle-next="#ss_next5" data-cycle-caption="#image-caption-NB910-94159" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0019.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0019.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions." alt="Western Blot: Autophagy Antibody Pack [NB910-94159] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0020.jpg" title="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94159] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0020.jpg" alt="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94159] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0022.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG5 was detected using NB110-53818." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94159-img0022.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG5 was detected using NB110-53818." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0015.jpg" title="Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0015.jpg" alt="Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94159-img0029.jpg" title="Simple Western: Autophagy Antibody Pack [NB910-94159] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Simple-Western-NB910-94159-img0029.jpg" alt="Simple Western: Autophagy Antibody Pack [NB910-94159] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94159-img0027.jpg" title="Flow Cytometry: Autophagy Antibody Pack [NB910-94159] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose" class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94159-img0027.jpg" alt="Flow Cytometry: Autophagy Antibody Pack [NB910-94159] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0030.jpg" title="Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0030.jpg" alt="Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0021.jpg" title="Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94159-img0021.jpg" alt="Immunohistochemistry: Autophagy Antibody Pack [NB910-94159] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0028.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94159] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0028.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94159] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0016.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94159] - Analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) and mouse ATG5 KO ES cell lysate (- cnt'l) (Lane 2). Atg5-/- ES cells from Dr. Noboru Mizushima [PMID: 11266458] Photo courtesy of Dr. Beth Levine, UT SW Med Center." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0016.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94159] - Analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) and mouse ATG5 KO ES cell lysate (- cnt'l) (Lane 2). Atg5-/- ES cells from Dr. Noboru Mizushima [PMID: 11266458] Photo courtesy of Dr. Beth Levine, UT SW Med Center." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0017.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0017.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0018.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG16L1. Lane 1: NIH/3T3 Lane 2: HeLa ." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0018.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG16L1. Lane 1: NIH/3T3 Lane 2: HeLa ." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0024.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94159] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893" class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0024.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94159] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0025.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94159-img0025.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94159] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A." class="big_thumb" /> </a> </div></div><div id="ss_next5" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-5" class="cycle-circle-pager"></div><div id="image-caption-NB910-94159"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Alligator">Al</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Primate">Pm</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Alligator">Al</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Primate">Pm</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span></div><div class="field not3column"><label>Validated by:</label><div class="value">    <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>     <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB910-94159","Product Name":"Autophagy Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Porcine, Alligator, Bovine, Canine, Primate","Applications":"WB, Simple Western, Flow, ICC\/IF, IHC, IP, KD","Source":"","Purification":"","Immunogen":"See individual datasheets","Citations":"-","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/autophagy-antibody-pack_nb910-94159\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/lc3-antibody-pack_nb910-40752">NB910-40752</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB910-40752" data-name="LC3 Antibody Pack" data-position="7" class="ecommerce_link" href="/products/lc3-antibody-pack_nb910-40752" title="LC3 Antibody Pack" ><span> LC3 Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="4 Publications specific to this product" href="/products/lc3-antibody-pack_nb910-40752#PublicationSection"><span class="publications"></span> (4 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>1 Pack</strong><span class="product_price" data-sku="NB910-40752-1Pack"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/lc3-antibody-pack_nb910-40752">NB910-40752</a> </div><div id="ss_prev6" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-6" data-cycle-prev="#ss_prev6" data-cycle-next="#ss_next6" data-cycle-caption="#image-caption-NB910-40752" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0026.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384." class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0026.jpg" title="<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384." alt="Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-40752-img0017.jpg" title="<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI." class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-MAP1LC3A-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-40752-img0017.jpg" alt="<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40752-img0027.jpg" title="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40752] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220] " class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Knockout-Validated-NB910-40752-img0027.jpg" alt="<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3 Antibody Pack [NB910-40752] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220] " class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0029.jpg" title="Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. Stronger co-localization between NeuN and LC3 is apparent at day 1 after SCI. Scale bar is 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license. LC3B Antibody [NB100-2220]" class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0029.jpg" alt="Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. Stronger co-localization between NeuN and LC3 is apparent at day 1 after SCI. Scale bar is 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license. LC3B Antibody [NB100-2220]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0030.jpg" title="Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Analysis in mouse renal tissue. Image from verifed customer review. LC3A Antibody [NB100-2331]" class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0030.jpg" alt="Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Analysis in mouse renal tissue. Image from verifed customer review. LC3A Antibody [NB100-2331]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0025.jpg" title="Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center." class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0025.jpg" alt="Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunohistochemistry-NB910-40752-img0021.jpg" title="Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384." class="big_lightbox"> <img src="//images.novusbio.com/images/LC3-MAP1LC3A-Antibody-Pack-Immunohistochemistry-NB910-40752-img0021.jpg" alt="Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384." class="big_thumb" /> </a> </div></div><div id="ss_next6" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-6" class="cycle-circle-pager"></div><div id="image-caption-NB910-40752"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Bacteria">Ba</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Primate">Pm</span>, <span title="Zebrafish">Ze</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Bacteria">Ba</span>, <span title="Bovine">Bv</span>, <span title="Canine">Ca</span>, <span title="Primate">Pm</span>, <span title="Zebrafish">Ze</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span>, <span class="application-tooltip-text" title="Knockout Validated">KO</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span>, <span class="application-tooltip-text" title="Knockout Validated">KO</span></div><div class="field not3column"><label>Validated by:</label><div class="value">  <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>   <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB910-40752","Product Name":"LC3 Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Porcine, Bacteria, Bovine, Canine, Primate, Zebrafish","Applications":"WB, Simple Western, ELISA, Flow, ICC\/IF, IHC, IP, WB, ChIP, KD, KO","Source":"","Purification":"","Immunogen":"For NB600-1384: Polyclonal LC3B Antibody was made to a synthetic peptide made to the N-terminal region of the human LC3B protein [Uniprot: Q9GZQ8].For NB100-2331: A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121) [Uniprot: Q9H492].For<span class=\"compare_read_more_click\">...(read more)<\/span><span class=\"compare_read_more\"> NB100-2220: Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100) [UniProt# Q9GZQ8].<\/span>","Citations":"4","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/lc3-antibody-pack_nb910-40752\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/ape-antibody-pack_nb100-909">NB100-909</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB100-909" data-name="APE Antibody Pack" data-position="8" class="ecommerce_link" href="/products/ape-antibody-pack_nb100-909" title="APE Antibody Pack" ><span> APE Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="4 Publications specific to this product" href="/products/ape-antibody-pack_nb100-909#PublicationSection"><span class="publications"></span> (4 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>4 Vials</strong><span class="product_price" data-sku="NB100-909"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/ape-antibody-pack_nb100-909">NB100-909</a> </div><div id="ss_prev7" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-7" data-cycle-prev="#ss_prev7" data-cycle-next="#ss_next7" data-cycle-caption="#image-caption-NB100-909" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0013.jpg" title="Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence." class="big_lightbox"> <img src="//images.novusbio.com/images/APE-Antibody-Pack-NB100-909-img0013.jpg" title="Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence." alt="Western Blot: APE Antibody Pack [NB100-909] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 (NB100-116) in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0014.jpg" title="Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue)." class="big_lightbox"> <img src="//images.novusbio.com/images/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0014.jpg" alt="Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - HeLa cells stained with anti-APE Antibody (NB100-116, Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue)." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg" title="Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/APE-Antibody-Pack-Immunohistochemistry-NB100-909-img0015.jpg" alt="Immunohistochemistry: APE Antibody Pack [NB100-909] - Immunohistochemical staining of APE1 using NB100-116. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/APE-Antibody-Pack-NB100-909-img0011.jpg" title="Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7." class="big_lightbox"> <img src="//images.novusbio.com/images/APE-Antibody-Pack-NB100-909-img0011.jpg" alt="Western Blot: APE Antibody Pack [NB100-909] - Analysis of APE1 using NB100-101 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0012.jpg" title="Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review." class="big_lightbox"> <img src="//images.novusbio.com/images/APE-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-909-img0012.jpg" alt="Immunocytochemistry/Immunofluorescence: APE Antibody Pack [NB100-909] - Detection of APE1 using NB100-101 in HeLa cells. Image from verified customer review." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/APE1-Antibody-Pack-Immunohistochemistry-NB100-909-img0005.jpg" title="Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101." class="big_lightbox"> <img src="//images.novusbio.com/images/APE1-Antibody-Pack-Immunohistochemistry-NB100-909-img0005.jpg" alt="Immunohistochemistry: APE1 Antibody Pack [NB100-909] - Staining of APE-ref-1 in prostate cancer, using NB100-101." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/APE-Antibody-Pack-Simple-Western-NB100-909-img0010.jpg" title="Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_lightbox"> <img src="//images.novusbio.com/images/APE-Antibody-Pack-Simple-Western-NB100-909-img0010.jpg" alt="Simple Western: APE Antibody Pack [NB100-909] - Lane view shows a specific band for APE1 using NB100-101 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 (using NB100-116), p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/APE-Antibody-Pack-Knockdown-Validated-NB100-909-img0018.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: APE Antibody Pack [NB100-909] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1 (using NB100-116), p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license." class="big_thumb" /> </a> </div></div><div id="ss_next7" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-7" class="cycle-circle-pager"></div><div id="image-caption-NB100-909"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Primate">Pm</span>, <span title="Rabbit">Rb</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Primate">Pm</span>, <span title="Rabbit">Rb</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span></div><div class="field not3column"><label>Validated by:</label><div class="value">    <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>     <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB100-909","Product Name":"APE Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Primate, Rabbit","Applications":"WB, Simple Western, ICC\/IF, IHC, IP, ChIP","Source":"","Purification":"","Immunogen":"See individual datasheets","Citations":"4","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/ape-antibody-pack_nb100-909\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/arnt-hif-1-beta-antibody-pack_nb100-982">NB100-982</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB100-982" data-name="ARNT/HIF-1 beta Antibody Pack" data-position="9" class="ecommerce_link" href="/products/arnt-hif-1-beta-antibody-pack_nb100-982" title="ARNT/HIF-1 beta Antibody Pack" ><span> ARNT/HIF-1 beta Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="4 Publications specific to this product" href="/products/arnt-hif-1-beta-antibody-pack_nb100-982#PublicationSection"><span class="publications"></span> (4 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>3 Vials</strong><span class="product_price" data-sku="NB100-982"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/arnt-hif-1-beta-antibody-pack_nb100-982">NB100-982</a> </div><div id="ss_prev8" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-8" data-cycle-prev="#ss_prev8" data-cycle-next="#ss_next8" data-cycle-caption="#image-caption-NB100-982" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0010.jpg" title="Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa." class="big_lightbox"> <img src="//images.novusbio.com/images/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0010.jpg" title="Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa." alt="Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Analysis of ARNT/HIF-1 beta using ARNT/HIF-1 beta antibody [NB100-110] from ARNT/HIF-1 beta antibody pack [NB100-982] in MCF7 whole cell lysate. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0005.jpg" title="Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of skin, epidermis using ARNT/HIF-1 beta antibody [NB100-110] from the ARNT/HIF-1 beta antibody pack [NB100-982]." class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0005.jpg" alt="Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of skin, epidermis using ARNT/HIF-1 beta antibody [NB100-110] from the ARNT/HIF-1 beta antibody pack [NB100-982]." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-982-img0009.jpg" title="Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Allele specific HIF-binding at the rs12814794 associated enhancer. B) Allele-specific qPCR for rs12814794 from DNA fragments isolated in HIF-ChIP experiments (HIF-1A and ARNT/HIF-1 beta) or input DNA from primary renal tubular cells (PTC, n = 4 individuals). DNA from individuals homozygous for the AA or GG genotype were used as positive controls. C) Quantification of the two different alleles (A or G) at rs12814794 in the ChIP-seq reads from HIF-1A and ARNT/HIF-1 beta antibody [NB100-110] immunoprecipitations (PTC #1 Fig 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1006872) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/ARNT-HIF-1-beta-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-982-img0009.jpg" alt="Chromatin Immunoprecipitation: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Allele specific HIF-binding at the rs12814794 associated enhancer. B) Allele-specific qPCR for rs12814794 from DNA fragments isolated in HIF-ChIP experiments (HIF-1A and ARNT/HIF-1 beta) or input DNA from primary renal tubular cells (PTC, n = 4 individuals). DNA from individuals homozygous for the AA or GG genotype were used as positive controls. C) Quantification of the two different alleles (A or G) at rs12814794 in the ChIP-seq reads from HIF-1A and ARNT/HIF-1 beta antibody [NB100-110] immunoprecipitations (PTC #1 Fig 3). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1006872) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0006.jpg" title="Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1a or HIF-2a expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1a-shRNA or HIF-2a-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. ARNT/HIF-1 beta expression was used as a loading control. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0006.jpg" alt="Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1a or HIF-2a expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1a-shRNA or HIF-2a-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. ARNT/HIF-1 beta expression was used as a loading control. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0007.jpg" title="Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A [NB100-480] and ARNT/HIF-1 beta [NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/ARNT-HIF-1-beta-Antibody-Pack-Western-Blot-NB100-982-img0007.jpg" alt="Western Blot: ARNT/HIF-1 beta Antibody Pack [NB100-982] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A [NB100-480] and ARNT/HIF-1 beta [NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight of ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0004.jpg" title="Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of human glioblastoma multi-forme utilizing the ARNT/HIF-1 beta antibody[NB100-124] from the ARNT/HIF-1 beta antibody pack [NB100-982]." class="big_lightbox"> <img src="//images.novusbio.com/images/HIF-1-beta-Antibody-Pack-Immunohistochemistry-NB100-982-img0004.jpg" alt="Immunohistochemistry: ARNT/HIF-1 beta Antibody Pack [NB100-982] - Staining of human glioblastoma multi-forme utilizing the ARNT/HIF-1 beta antibody[NB100-124] from the ARNT/HIF-1 beta antibody pack [NB100-982]." class="big_thumb" /> </a> </div></div><div id="ss_next8" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-8" class="cycle-circle-pager"></div><div id="image-caption-NB100-982"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Bovine">Bv</span>, <span title="Ferret">Ma</span>, <span title="Sheep">Sh</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Bovine">Bv</span>, <span title="Ferret">Ma</span>, <span title="Sheep">Sh</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Chromatin Immunoprecipitation">ChIP</span></div><div class="field not3column"><label>Host:</label><div class="value">Mouse</div></div><div class="forcol3 bottom_margin"><strong>Host:</strong> Mouse</div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB100-982","Product Name":"ARNT\/HIF-1 beta Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Bovine, Ferret, Sheep","Applications":"WB, ICC\/IF, IHC, IP, WB, ChIP","Source":"","Purification":"","Immunogen":"See individual datasheets.","Citations":"4","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/arnt-hif-1-beta-antibody-pack_nb100-982\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/survivin-antibody-pack_nb100-911">NB100-911</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB100-911" data-name="Survivin Antibody Pack" data-position="10" class="ecommerce_link" href="/products/survivin-antibody-pack_nb100-911" title="Survivin Antibody Pack" ><span> Survivin Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="7 Publications specific to this product" href="/products/survivin-antibody-pack_nb100-911#PublicationSection"><span class="publications"></span> (7 Publications)</a> </div> <div class="segment" style="float:right;"> <strong>5 Vials</strong><span class="product_price" data-sku="NB100-911-1Pack"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/survivin-antibody-pack_nb100-911">NB100-911</a> </div><div id="ss_prev9" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-9" data-cycle-prev="#ss_prev9" data-cycle-next="#ss_next9" data-cycle-caption="#image-caption-NB100-911" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/Survivin-Antibody-Pack-RNAscope-compatible-NB100-911-img0006.jpg" title="Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells." class="big_lightbox"> <img src="//images.novusbio.com/images/Survivin-Antibody-Pack-RNAscope-compatible-NB100-911-img0006.jpg" title="Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells." alt="Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0008.jpg" title="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Survivin Antibody Pack [NB100-911] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0008.jpg" alt="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Survivin Antibody Pack [NB100-911] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0010.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Survivin Antibody Pack [NB100-911] - Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as control or for survivin. A: A pronounced decrease of survivin protein levels was measured by immunoblotting in SW1353 and Hs819.T. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license. Survivin Antibody (60.11) [NB500-238]" class="big_lightbox"> <img src="//images.novusbio.com/images/Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0010.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: Survivin Antibody Pack [NB100-911] - Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as control or for survivin. A: A pronounced decrease of survivin protein levels was measured by immunoblotting in SW1353 and Hs819.T. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license. Survivin Antibody (60.11) [NB500-238]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-911-img0007.jpg" title="Immunocytochemistry/Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201]." class="big_lightbox"> <img src="//images.novusbio.com/images/Survivin-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-911-img0007.jpg" alt="Immunocytochemistry/Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201]." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-NB100-911-img0005.jpg" title="<b>Independent Antibodies Validation. </b>Immunohistochemistry: Survivin Antibody Pack [NB100-911] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/Survivin-Antibody-Pack-Immunohistochemistry-NB100-911-img0005.jpg" alt="<b>Independent Antibodies Validation. </b>Immunohistochemistry: Survivin Antibody Pack [NB100-911] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Survivin-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-911-img0009.jpg" title="Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237]." class="big_lightbox"> <img src="//images.novusbio.com/images/Survivin-Antibody-Pack-Immunohistochemistry-Paraffin-NB100-911-img0009.jpg" alt="Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237]." class="big_thumb" /> </a> </div></div><div id="ss_next9" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-9" class="cycle-circle-pager"></div><div id="image-caption-NB100-911"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="ELISA">ELISA</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span></div><div class="field not3column"><label>Validated by:</label><div class="value"><span><a href="/5-pillars-validation" class="AtlasAntibody" title="Independent Antibodies"> </a></span>   <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong> <span><a href="/5-pillars-validation" class="AtlasAntibody" title="Independent Antibodies"> </a></span>   <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB100-911","Product Name":"Survivin Antibody Pack","Description":"","Reactivity":"Human, Mouse","Applications":"WB, ELISA, Flow, ICC\/IF, IHC","Source":"","Purification":"","Immunogen":"See individual datasheets.","Citations":"7","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/survivin-antibody-pack_nb100-911\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/pluripotent-stem-cell-transcription-factor-antibody-pack_nbp1-42823">NBP1-42823</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NBP1-42823" data-name="Pluripotent Stem Cell Transcription Factor Antibody Pack" data-position="11" class="ecommerce_link" href="/products/pluripotent-stem-cell-transcription-factor-antibody-pack_nbp1-42823" title="Pluripotent Stem Cell Transcription Factor Antibody Pack" ><span> Pluripotent Stem Cell Transcription Factor Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="1 Review for this product" href="/products/pluripotent-stem-cell-transcription-factor-antibody-pack_nbp1-42823#ReviewsSection"><span class="stars5"></span> (1 Review)</a> </div> <div class="segment"> <a title="1 Publication specific to this product" href="/products/pluripotent-stem-cell-transcription-factor-antibody-pack_nbp1-42823#PublicationSection"><span class="publications"></span> (1 Publication)</a> </div> <div class="segment" style="float:right;"> <strong>4 Vials</strong><span class="product_price" data-sku="NBP1-42823"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/pluripotent-stem-cell-transcription-factor-antibody-pack_nbp1-42823">NBP1-42823</a> </div><div id="ss_prev10" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-10" data-cycle-prev="#ss_prev10" data-cycle-next="#ss_next10" data-cycle-caption="#image-caption-NBP1-42823" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42823-img0014.jpg" title="Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NBP1-42823-img0014.jpg" title="Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license." alt="Immunocytochemistry/Immunofluorescence: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-NBP1-42823-img0016.jpg" title="Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license. OCT4 Antibody [NB100-2379]" class="big_lightbox"> <img src="//images.novusbio.com/images/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-NBP1-42823-img0016.jpg" alt="Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license. OCT4 Antibody [NB100-2379]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0013.jpg" title="Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml)." class="big_lightbox"> <img src="//images.novusbio.com/images/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0013.jpg" alt="Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml)." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0015.jpg" title="Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license. Nanog Antibody [NB100-58842]" class="big_lightbox"> <img src="//images.novusbio.com/images/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0015.jpg" alt="Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license. Nanog Antibody [NB100-58842]" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0011.jpg" title="Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds" class="big_lightbox"> <img src="//images.novusbio.com/images/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0011.jpg" alt="Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0017.jpg" title="Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - SOX2 staining (NB110-37235) of human uterus, endometrial glands." class="big_lightbox"> <img src="//images.novusbio.com/images/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0017.jpg" alt="Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - SOX2 staining (NB110-37235) of human uterus, endometrial glands." class="big_thumb" /> </a> </div></div><div id="ss_next10" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-10" class="cycle-circle-pager"></div><div id="image-caption-NBP1-42823"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span></div><div class="field not3column"><label>Host:</label><div class="value">Rabbit Polyclonal</div></div><div class="forcol3 bottom_margin"><strong>Host:</strong> Rabbit Polyclonal</div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NBP1-42823","Product Name":"Pluripotent Stem Cell Transcription Factor Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat","Applications":"WB, Flow, ICC\/IF, IHC","Source":"","Purification":"","Immunogen":"See individual datasheets.","Citations":"1","Purity":"Immunogen affinity purified","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/pluripotent-stem-cell-transcription-factor-antibody-pack_nbp1-42823\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div><div class="new-search-result search-result-wrapper" > <div class="name"><span> <div class="catalog_number_wrapper not3column"> <a href="/products/autophagy-antibody-pack_nb910-94877">NB910-94877</a> </div><h2 class="col3_hdr"><a data-brand="novus" data-id="NB910-94877" data-name="Autophagy Antibody Pack" data-position="12" class="ecommerce_link" href="/products/autophagy-antibody-pack_nb910-94877" title="Autophagy Antibody Pack" ><span> Autophagy Antibody Pack</span></a></div></h2> <div class="sub-header"> <div class="flourish_left"></div> <div class="flourish_right"></div><div class="prod_details_section"> <div class="segment"> <a title="1 Publication specific to this product" href="/products/autophagy-antibody-pack_nb910-94877#PublicationSection"><span class="publications"></span> (1 Publication)</a> </div> <div class="segment" style="float:right;"> <strong>6 Vials</strong><span class="product_price" data-sku="NB910-94877"></span> </div> </div> </div> <div class="images"> <div class="catalog_number_wrapper_col3 forcol3"> <a href="/products/autophagy-antibody-pack_nb910-94877">NB910-94877</a> </div><div id="ss_prev11" class="cycle-pager"><div class="pager_left_on"></div></div><div class="cycle-slideshow slideshows" data-cycle-fx="scrollHorz" data-cycle-timeout="0" data-cycle-pager="#cycle-circle-pager-11" data-cycle-prev="#ss_prev11" data-cycle-next="#ss_next11" data-cycle-caption="#image-caption-NB910-94877" data-cycle-caption-template="Image {{slideNum}} / {{slideCount}}" data-cycle-slides=".big_thumb_container" ><div class="big_thumb_container"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0027.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0027.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions." alt="Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0028.jpg" title="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0028.jpg" alt="<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0031.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG antibody (NB110-53818) used. " class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0031.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG antibody (NB110-53818) used. " class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0025.jpg" title="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0025.jpg" alt="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94877-img0037.jpg" title="Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Simple-Western-NB910-94877-img0037.jpg" alt="Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94877-img0035.jpg" title="Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose" class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94877-img0035.jpg" alt="Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0038.jpg" title="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0038.jpg" alt="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0029.jpg" title="<b>Biological Strategies Validation. </b>Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0029.jpg" alt="<b>Biological Strategies Validation. </b>Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0036.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0036.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0040.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0040.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0041.jpg" title="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0041.jpg" alt="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0017.jpg" title="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0017.jpg" alt="<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A." class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0032.jpg" title="Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893" class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0032.jpg" alt="Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893" class="big_thumb" /> </a> </div><div class="big_thumb_container" style="visibility:hidden;"> <a href="//images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0018.jpg" title="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine." class="big_lightbox"> <img src="//images.novusbio.com/images/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0018.jpg" alt="Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine." class="big_thumb" /> </a> </div></div><div id="ss_next11" class="cycle-pager"><div class="pager_right_on"></div></div><div id="cycle-circle-pager-11" class="cycle-circle-pager"></div><div id="image-caption-NB910-94877"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Alligator">Al</span>, <span title="Bovine">Bv</span>, <span title="Primate">Pm</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span>, <span title="Mouse">Mu</span>, <span title="Rat">Rt</span>, <span title="Porcine">Po</span>, <span title="Alligator">Al</span>, <span title="Bovine">Bv</span>, <span title="Primate">Pm</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Western Blot">WB</span>, <span class="application-tooltip-text" title="Simple Western">Simple Western</span>, <span class="application-tooltip-text" title="Flow Cytometry">Flow</span>, <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span>, <span class="application-tooltip-text" title="Immunohistochemistry">IHC</span>, <span class="application-tooltip-text" title="Immunoprecipitation">IP</span>, <span class="application-tooltip-text" title="Knockdown Validated">KD</span></div><div class="field not3column"><label>Validated by:</label><div class="value">  <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div></div><div class="forcol3 bottom_margin"><strong>Validated by:</strong>   <span><a href="/5-pillars-validation" class="AtlasBiological" title="Biological Strategies"> </a></span> <span><a href="/5-pillars-validation" class="AtlasGenetic" title="Genetic Strategies"> </a></span>    </div> </div> <div class="compare_block"><input type="checkbox" class="search_compare_checkbox" data-compare='{"Catalog #":"NB910-94877","Product Name":"Autophagy Antibody Pack","Description":"","Reactivity":"Human, Mouse, Rat, Porcine, Alligator, Bovine, Primate","Applications":"WB, Simple Western, Flow, ICC\/IF, IHC, IP, KD","Source":"","Purification":"","Immunogen":"See individual datasheets","Citations":"1","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/autophagy-antibody-pack_nb910-94877\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div></div><div class="lower_framework"> <div class="search_pager"><div class="searchImage_backGrey" style="display:inline-block;zoom:1;width:6px;height:11px;background:url(//resources.rndsystems.com/images/search/searchSprite03-31-16.png) -18px -169px"></div> page <input type="text" value="1" class="search_pager_text" data-url="/product-type/antibody-packs?page="> of <span class="search_total_pages">6</span> <div class="search_page forward" style="display:inline-block;zoom:1"><a href="/product-type/antibody-packs?page=2" rel="nofollow" class="active"><div class="searchImage_forward" 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