Search results for: ddPCR

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ddPCR</title> <meta name="description" content="Search results for: ddPCR"> <meta name="keywords" content="ddPCR"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="ddPCR"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 3</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: ddPCR</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Quantitative Evaluation of Endogenous Reference Genes for ddPCR under Salt Stress Using a Moderate Halophile</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Qinghua%20Xing">Qinghua Xing</a>, <a href="https://publications.waset.org/abstracts/search?q=Noha%20M.%20Mesbah"> Noha M. Mesbah</a>, <a href="https://publications.waset.org/abstracts/search?q=Haisheng%20Wang"> Haisheng Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun%20Li"> Jun Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Baisuo%20Zhao"> Baisuo Zhao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Droplet digital PCR (ddPCR) is being increasingly adopted for gene detection and quantification because of its higher sensitivity and specificity. According to previous observations and our lab data, it is essential to use endogenous reference genes (RGs) when investigating gene expression at the mRNA level under salt stress. This study aimed to select and validate suitable RGs for gene expression under salt stress using ddPCR. Six candidate RGs were selected based on the tandem mass tag (TMT)-labeled quantitative proteomics of Alkalicoccus halolimnae at four salinities. The expression stability of these candidate genes was evaluated using statistical algorithms (geNorm, NormFinder, BestKeeper and RefFinder). There was a small fluctuation in cycle threshold (Ct) value and copy number of the pdp gene. Its expression stability was ranked in the vanguard of all algorithms, and was the most suitable RG for quantification of expression by both qPCR and ddPCR of A. halolimnae under salt stress. Single RG pdp and RG combinations were used to normalize the expression of ectA, ectB, ectC, and ectD under four salinities. The present study constitutes the first systematic analysis of endogenous RG selection for halophiles responding to salt stress. This work provides a valuable theory and an approach reference of internal control identification for ddPCR-based stress response models. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=endogenous%20reference%20gene" title="endogenous reference gene">endogenous reference gene</a>, <a href="https://publications.waset.org/abstracts/search?q=salt%20stress" title=" salt stress"> salt stress</a>, <a href="https://publications.waset.org/abstracts/search?q=ddPCR" title=" ddPCR"> ddPCR</a>, <a href="https://publications.waset.org/abstracts/search?q=RT-qPCR" title=" RT-qPCR"> RT-qPCR</a>, <a href="https://publications.waset.org/abstracts/search?q=Alkalicoccus%20halolimnae" title=" Alkalicoccus halolimnae"> Alkalicoccus halolimnae</a> </p> <a href="https://publications.waset.org/abstracts/165112/quantitative-evaluation-of-endogenous-reference-genes-for-ddpcr-under-salt-stress-using-a-moderate-halophile" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165112.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">104</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Adaptation Mechanisms of the Polyextremophile Natranaerobius Thermophilus to Saline-Alkaline-Hermal Environments</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Qinghua%20Xing">Qinghua Xing</a>, <a href="https://publications.waset.org/abstracts/search?q=Xinyi%20Tao"> Xinyi Tao</a>, <a href="https://publications.waset.org/abstracts/search?q=Haisheng%20Wang"> Haisheng Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Baisuo%20Zhao"> Baisuo Zhao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The first true anaerobic, halophilic alkali thermophile, Natranaerobius thermophilus DSM 18059T, serves as a valuable model for studying cellular adaptations to saline, alkaline and thermal extremes. To uncover the adaptive strategies employed by N. thermophilus in coping with these challenges, we conducted a comprehensive iTRAQ-based quantitative proteomic analysis under different conditions of salinity (3.5 M vs. 2.5 M Na+), pH (pH 9.6 vs. pH 8.6), and temperature (52°C vs. 42°C). The increased intracellular accumulation of glycine betaine, through both synthesis and transport, plays a critical role in N. thermophilus' adaptation to these combined stresses. Under all three stress conditions, the up-regulation of Trk family proteins responsible for K+ transport is observed. Intracellular K+ concentration rises in response to salt and pH levels. Multiple types of Na+/H+ antiporter (NhaC family, Mrp family and CPA family) and a diverse range of FOF1-ATP synthase are identified as vital components for maintaining ionic balance under different stress conditions. Importantly, proteins involved in amino acid metabolism, carbohydrate metabolism, ABC transporters, signaling and chemotaxis, as well as biological macromolecule repair and protection, exhibited significant up-regulation in response to these extreme conditions. These metabolic pathways emerge as critical factors in N. thermophilus' adaptation mechanisms under extreme environmental stress. To validate the proteomic data, ddPCR analysis confirmed changes in mRNA expression, thereby corroborating the up-regulation and down-regulation patterns of 19 co-up-regulated and 36 key proteins under saline, alkaline and thermal stresses. This research enriches our understanding of the complex regulatory systems that enable polyextremophiles to survive in combined extreme conditions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=polyextremophiles" title="polyextremophiles">polyextremophiles</a>, <a href="https://publications.waset.org/abstracts/search?q=natranaerobius%20thermophilus" title=" natranaerobius thermophilus"> natranaerobius thermophilus</a>, <a href="https://publications.waset.org/abstracts/search?q=saline-%20alkaline-%20thermal%20stresses" title=" saline- alkaline- thermal stresses"> saline- alkaline- thermal stresses</a>, <a href="https://publications.waset.org/abstracts/search?q=combined%20extremes" title=" combined extremes"> combined extremes</a> </p> <a href="https://publications.waset.org/abstracts/185460/adaptation-mechanisms-of-the-polyextremophile-natranaerobius-thermophilus-to-saline-alkaline-hermal-environments" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/185460.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">55</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Treatment of Non-Small Cell Lung Cancer (NSCLC) With Activating Mutations Considering ctDNA Fluctuations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Moiseenko%20F.%20V.">Moiseenko F. V.</a>, <a href="https://publications.waset.org/abstracts/search?q=Volkov%20N.%20M."> Volkov N. M.</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhabina%20A.%20S."> Zhabina A. S.</a>, <a href="https://publications.waset.org/abstracts/search?q=Stepanova%20E.%20O."> Stepanova E. O.</a>, <a href="https://publications.waset.org/abstracts/search?q=Kirillov%20A.%20V."> Kirillov A. V.</a>, <a href="https://publications.waset.org/abstracts/search?q=Myslik%20A.%20V."> Myslik A. V.</a>, <a href="https://publications.waset.org/abstracts/search?q=Artemieva%20E.%20V."> Artemieva E. V.</a>, <a href="https://publications.waset.org/abstracts/search?q=Agranov%20I.%20R."> Agranov I. R.</a>, <a href="https://publications.waset.org/abstracts/search?q=Oganesyan%20A.%20P."> Oganesyan A. P.</a>, <a href="https://publications.waset.org/abstracts/search?q=Egorenkov%20V.%20V."> Egorenkov V. V.</a>, <a href="https://publications.waset.org/abstracts/search?q=Abduloeva%20N.%20H."> Abduloeva N. H.</a>, <a href="https://publications.waset.org/abstracts/search?q=Aleksakhina%20S.%20Yu."> Aleksakhina S. Yu.</a>, <a href="https://publications.waset.org/abstracts/search?q=Ivantsov%20A.%20O."> Ivantsov A. O.</a>, <a href="https://publications.waset.org/abstracts/search?q=Kuligina%20E.%20S."> Kuligina E. S.</a>, <a href="https://publications.waset.org/abstracts/search?q=Imyanitov%20E.%20N."> Imyanitov E. N.</a>, <a href="https://publications.waset.org/abstracts/search?q=Moiseyenko%20V.%20M."> Moiseyenko V. M.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Analysis of ctDNA in patients with NSCLC is an emerging biomarker. Multiple research efforts of quantitative or at least qualitative analysis before and during the first periods of treatment with TKI showed the prognostic value of ctDNA clearance. Still, these important results are not incorporated in clinical standards. We evaluated the role of ctDNA in EGFR-mutated NSCLC receiving first-line TKI. Firstly, we analyzed sequential plasma samples from 30 patients that were collected before intake of the first tablet (at baseline) and at 6, 12, 24, 36, and 48 hours after the “starting point.” EGFR-M+ allele was measured by ddPCR. Afterward, we included sequential qualitative analysis of ctDNA with cobas® EGFR Mutation Test v2 from 99 NSCLC patients before the first dose, after 2 and 4 months of treatment, and on progression. Early response analysis showed the decline of EGFR-M+ level in plasma within the first 48 hours of treatment in 11 subjects. All these patients showed objective tumor response. 10 patients showed either elevation of EGFR-M+ plasma concentration (n = 5) or stable content of circulating EGFR-M+ after the start of the therapy (n = 5); only 3 of these patients achieved an objective response (p = 0.026) when compared to the former group). The rapid decline of plasma EGFR-M+ DNA concentration also predicted for longer PFS (13.7 vs. 11.4 months, p = 0.030). Long-term ctDNA monitoring showed clinically significant heterogeneity of EGFR-mutated NSCLC treated with 1st line TKIs in terms of progression-free and overall survival. Patients without detectable ctDNA at baseline (N = 32) possess the best prognosis on the duration of treatment (PFS: 24.07 [16.8-31.3] and OS: 56.2 [21.8-90.7] months). Those who achieve clearance after two months of TKI (N = 42) have indistinguishably good PFS (19.0 [13.7 – 24.2]). Individuals who retain ctDNA after 2 months (N = 25) have the worst prognosis (PFS: 10.3 [7.0 – 13.5], p = 0.000). 9/25 patients did not develop ctDNA clearance at 4 months with no statistical difference in PFS from those without clearance at 2 months. Prognostic heterogeneity of EGFR-mutated NSCLC should be taken into consideration in planning further clinical trials and optimizing the outcomes of patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=NSCLC" title="NSCLC">NSCLC</a>, <a href="https://publications.waset.org/abstracts/search?q=EGFR" title=" EGFR"> EGFR</a>, <a href="https://publications.waset.org/abstracts/search?q=targeted%20therapy" title=" targeted therapy"> targeted therapy</a>, <a href="https://publications.waset.org/abstracts/search?q=ctDNA" title=" ctDNA"> ctDNA</a>, <a href="https://publications.waset.org/abstracts/search?q=prognosis" title=" prognosis"> prognosis</a> </p> <a href="https://publications.waset.org/abstracts/182095/treatment-of-non-small-cell-lung-cancer-nsclc-with-activating-mutations-considering-ctdna-fluctuations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/182095.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">55</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); 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Search results for: ddPCR