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<div>316</div> <div>views</div> </div> </div> <div class="content"> <div class="title mini header"> <a href="/p/9160011/"> Normalization RNA-seq for multiple plates </a> </div> <div class="extra right floated"> <div class="left floated"> <a class="ptag" href="/tag/Normalization/"> Normalization </a> <a class="ptag" href="/tag/DESeq2/"> DESeq2 </a> <a class="ptag" href="/tag/RNASeqData/"> RNASeqData </a> <a class="ptag" href="/tag/edgeR/"> edgeR </a> </div> <div class="right floated muted"> updated 4 months ago by <a itemprop="author" itemscope itemtype="https://schema.org/Person" href="/u/13662/"> <span itemprop="name">ATpoint</span> </a> ★ 4.7k • written 4 months ago by <a itemprop="author" itemscope itemtype="https://schema.org/Person" href="/u/78779/"> <span itemprop="name">Pimpa</span> </a> • 0 </div> </div> </div> </div> <div class="post open item" data-value="9159613"> <div class="stats"> <div class="ui mini stat left label"> <div>1</div> <div>vote</div> </div> <div class="ui 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href="/u/79621/">boidnoid </a> <span> • 0 </span> <div class="muted" style=""> I was having the same problem. You can check the answer in a [previous post][1]. Specifically, reinstalling the devel version from GitHub … </div> </div> <div class="item spaced"> <a href="/p/9161353/"> Answer: How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differenti</a> by <a href="/u/5106/">James W. MacDonald </a> <span> <i class="ui muted bolt icon"></i> 68k </span> <div class="muted" style=""> The short answer is yes. When you compute pseudo-bulk estimates, the counts/gene are expected to be somewhat proportional to the number of … </div> </div> <div class="item spaced"> <a href="/p/9161352/"> Comment: displaying the differential expression with deseq2 over 4X2 samples with the in</a> by <a href="/u/79619/">Leonard </a> <span> • 0 </span> <div class="muted" style=""> Visualize subject contributions to overall differential expression using: Volcano plots/scatter plots (subject-colored). Heatmaps (subject… </div> </div> <div class="item spaced"> <a href="/p/9161350/"> Comment: Plot title for PCA in DESeq2</a> by <a href="/u/78232/">AL </a> <span> • 0 </span> <div class="muted" style=""> Thank you! </div> </div> <div class="item spaced"> <a href="/p/9161349/"> Answer: Interpreting DESEQ2 interaction term and inclusion of covariate</a> by <a href="/u/14086/">swbarnes2 </a> <span> ★ 1.4k </span> <div class="muted" style=""> "ecotypehigh.rearing_high" will basically do a 4 group comparison, and tell you if any genes that change going from high to low change diff… </div> </div> </div> </div> </div> <div class="ui divider"></div> <div class="event"> <div class="ui container"> <div>Votes </div> <div class="ui relaxed list" > <div class="item spaced" > <a href="/p/9161347/">Interpreting DESEQ2 interaction term and inclusion of covariate</a> </div> <div class="item spaced" > <a href="/p/112072/">A: Seurat/DESeq2 - single cell RNA-seq differential expression</a> </div> <div class="item spaced" > <a href="/p/9161339/">Answer: Plot title for PCA in DESeq2</a> </div> <div class="item spaced" > <a href="/p/60441/">A: DESeq2: detect significantly increasing across an ordinal variable</a> </div> <div class="item spaced" > <a href="/p/9161345/">Answer: DEseq2 design for additive treatment</a> </div> </div> </div> </div> <div class="ui divider"></div> <div class="event"> <div class="ui container"> <span>Awards</span> <a href="/b/list/"> • All <i class="ui angle small double right icon"></i></a> <div class="ui relaxed list" > <div class="item" style=""> <span> <a href="/b/view/popular-question/">Popular Question <i class="eye icon"></i></a> to <a href="/u/179/"> Gordon Smyth</a> <span> <i class="ui muted bolt icon"></i> 52k </span> </span> </div> <div class="item" style=""> <span> <a href="/b/view/popular-question/">Popular Question <i class="eye icon"></i></a> to <a href="/u/5106/"> James W. 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