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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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$j('#navList').prepend(activeNav); } </script></div></div></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_311f"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-resupply-packs.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.320.high.jpg/1729752198775.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.480.high.jpg/1729752198775.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.full.high.jpg/1729752198775.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.320.high.jpg/1729752198775.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.480.high.jpg/1729752198775.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.320.medium.jpg/1729752198775.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.620.high.jpg/1729752198775.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.320.low.jpg/1729752198775.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_311f/foregroundimg.img.480.medium.jpg/1729752198775.jpg" alt='Invitrogen precast Bis-Tris gels' title='Invitrogen precast Bis-Tris gels'></picture><style> #cq-image-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_311f img{ width:100%; max-width: 480px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_311f .image-contain" + "," + "#cq-image-jsp-"+"319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_311f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><p><a id="top"></a>NuPAGE Bis-Tris Gels and Bolt Bis-Tris Plus Gels are high-performance precast polyacrylamide gels developed to provide optimal separation of a wide range of protein sizes under denaturing conditions. The neutral pH of the Bis-Tris chemistry helps minimize protein modifications and reduce protein degradation. As a result, NuPAGE and Bolt gels are a good choice for any application where protein integrity is crucial.</p></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p><a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-resupply-packs.html">Restock with resupply packs</a></p></div></div></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h3"> On this page: </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><a href="#specifications">NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gel specifications</a></li><li><a href="#degradation">Reduced protein degradation and sharp, straight bands</a></li><li><a href="#loading">WedgeWells format gels with high loading capacity wells</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul><li><a href="#order">Ordering</a></li><li><a href="#related-resources">Related resources</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="heading section"><h2 id="specifications"> NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gel specifications </h2></div><div class="text parbase section"><p>NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels offer:</p><ul><li><b>Excellent protein integrity—</b>neutral pH helps reduce protein degradation</li><li><b>Wide range of molecular weight separation—</b>select the right gel and running buffer to achieve the optimal separation of your proteins</li><li><b>Fast run times—</b>separate your proteins in 20–35 minutes</li><li><b>Long shelf life—</b>up to 16 months at room temperature</li><li><b>High-capacity wells—</b>WedgeWell format wells are wedge-shaped wells that double the well sample loading capacity and make it easy to load your sample (no specialized pipette tips required)</li></ul></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1174420041"><tbody><tr><th width="30%"> </th><th width="35%"><b>NuPAGE Bis-Tris</b></th><th width="35%"><b>Bolt Bis-Tris Plus</b></th></tr><tr><td><b>Select for</b></td><td>Higher sample throughput, increased sample volume capacity</td><td>Faster run times, increased sample volume capacity</td></tr><tr><td><b>Available gel sizes</b></td><td>Mini: 8 cm x 8 cm (1.0 or 1.5 mm thick)<br> Midi: 8 cm x 13 cm (1.0 mm thick)</td><td>Mini: 8 cm x 8 cm (1.0 mm thick)</td></tr><tr><td><b>Available Wells configurations*</b></td><td>WedgeWell format Midi: 12+2, 20, 26 wells<br> WedgeWell format Mini: 10, 12, 15, 17 wells<br> Mini: 1, 9, 10, 12, 15, 17, 2D-well, IPG wells<br> Midi: 12+2, 20, 26 wells</td><td>WedgeWell format Mini: 10, 12, 15, 17 well</td></tr><tr><td><b>Storage conditions</b></td><td colspan="2" style="text-align: center;">4–25°C</td></tr><tr><td><b>Shelf life</b></td><td>up to 16 months**</td><td>16 months</td></tr><tr><td><b>High lot-to-lot consistency</b></td><td>Coefficient of variation (CV) of only 2% for Rf values (migration)</td><td>Coefficient of variation (CV) of only 2% for Rf values (migration)</td></tr><tr><td><b>Recommended sample buffer</b></td><td>NuPAGE LDS Sample Buffer</td><td>Bolt LDS sample buffer</td></tr><tr><td><b>Recommended running buffers</b></td><td>NuPAGE MES SDS or NuPAGE MOPS SDS Running Buffer</td><td>Bolt MES SDS or Bolt MOPS SDS running buffer</td></tr><tr><td><b>Run time</b></td><td>35 min (MES buffer)<br> 50 min (MOPS Buffer)</td><td>20 min (MES buffer)<br> 35 min (MOPS buffer)</td></tr><tr><td><b>Recommended transfer buffer</b></td><td>NuPAGE Transfer Buffer</td><td>Bolt Transfer Buffer</td></tr><tr><td><b>Gel chemistry</b></td><td>Bis-Tris</td><td>Bis-Tris Plus</td></tr><tr><td><b>Available polyacrylamide concentrations</b></td><td colspan="2" style="text-align: center;">8%, 10%, 12%, 4–12%</td></tr><tr><td><b>Separation range</b></td><td colspan="2" style="text-align: center;">15 kDA to 260 kDa (MOPS buffer)<br> 3.5 kDA to 160 kDa (MES buffer)</td></tr><tr><td><b>For use with (equipment) mini gels</b></td><td colspan="2" style="text-align: center;"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/mini-gel-tank.html">Mini Gel Tank</a> or <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/xcell-surelock-mini-vertical-electrophoresis-system.html">XCell SureLock Mini-Cell</a></td></tr><tr><td><b>For use with (equipment) midi gels</b></td><td><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/surelock-tandem-midi-gel-tank.html">SureLock Tandem Midi Gel Tank</a>, <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/xcell4-surelock-midi-cell-electrophoresis-system.html">Invitrogen XCell4 SureLock Midi-Cell</a> or Bio-Rad Criterion (with adapters only)</td><td>Not available in midi size</td></tr><tr><td><b>Mode of separation</b></td><td colspan="2" style="text-align: center;">Molecular weight</td></tr><tr><td><b>Application</b></td><td colspan="2" style="text-align: center;">SDS-PAGE</td></tr></tbody></table></div><p>*Not all percentages are available in every well type<br> **Shelf life varies depending on gel format</p></div><div class="heading section"><h3><br>Benefits of the Bis-Tris and Bis-Tris plus gel chemistry </h3></div><div class="text parbase section"><p>NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend using buffers containing SDS. The gels can be run using MES SDS running buffer to better resolve smaller proteins (1–200 kDa) and MOPS SDS running buffer to resolve medium- to large-size proteins (14–260 kDa).</p><h4>Bis-Tris Gels use a Bis-Tris discontinuous buffer system involving three ions:</h4><ul><li>Chloride (–) is from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are bis-tris (+) and Cl (–) (pH 6.4).</li><li>MES or MOPS (–) serves as the trailing ion in the running buffer. The running buffer ions are tris (+), MOPS/MES (–) and dodecylsulfate (pH 7.3–7.7).</li><li>Bis-Tris (+) is the common ion present in the gel buffer and running buffer. The combination of lower pH gel buffer (pH 6.4) and the running buffer (pH 7.3–7.7) results in a significantly lower operating pH of 7 during electrophoresis</li></ul></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="degradation"> Reduced protein degradation and sharp, straight bands </h2></div><div class="heading section"><h3> Optimized performance of NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels </h3></div><div class="text parbase section"><p>Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels are Bis-Tris HCl buffered (pH 6.4) and have an operating pH of about 7.0. This neutral pH paired with a unique, gentle sample preparation protocol means your protein samples always reside in mild, nonacidic conditions, preserving the integrity of the proteins and minimizing protein modifications (see next section). This helps to ensure highly sensitive, accurate western blot results every time.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_2df5"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.320.medium.jpg/1729752199780.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.480.high.jpg/1729752199780.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.620.high.jpg/1729752199780.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.full.high.jpg/1729752199780.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.320.high.jpg/1729752199780.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.480.high.jpg/1729752199780.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.320.medium.jpg/1729752199780.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.620.high.jpg/1729752199780.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.320.low.jpg/1729752199780.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_2df5/foregroundimg.img.480.medium.jpg/1729752199780.jpg" alt='Western blot analysis using a Bolt Bis-Tris Plus gel compared to tris-glycine gels' title='Results from Bolt Bis-Tris Plus gels western transfer sensitivity experiment'></picture><style> #cq-image-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_2df5 img{ width:100%; max-width: 640px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_2df5 .image-contain" + "," + "#cq-image-jsp-"+"319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_2df5 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"></div></div><div style="clear:both"></div><div class="text parbase section"><p><small><b>Figure 1. Greater sensitivity with Bolt Bis-Tris Plus gels.</b> Total cell extracts from A431 cells were transferred to <a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/membranes-transfer-buffers-western-blotting.html">NC and PVDF membranes</a> from a 4–12% Bolt Bis-Tris Plus gel, and 4–20% Tris-glycine precast gel using the <a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/transfer-proteins-western-blot/iblot-dry-blotting-system.html">iBlot 2 Gel Transfer Device</a>. The cells were treated with 100 ng/mL of human epidermal growth factor (hEGF) to up-regulate expression of the phospho-EGF receptor. The protein loads of the cell extracts ranged from 20 μg to 1.2 μg of extract. The blots were processed on the <a href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/ibind-western-system.html">iBind Western System</a> using a 1:200 dilution of Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb (Cell Signaling Technology) and a 1:2,000 dilution of anti-mouse HRP secondary antibody.</small></p></div><div class="text parbase section"><h3><br> Excellent sample integrity</h3><p>Sample preparation is a crucial step in successful protein analysis. The pH of the traditional Laemmli-style sample buffer changes from 6.8 to 5.2 when heated at 100°C. This lower pH is known to induce aspartyl-prolyl (Asp-Pro) peptide bond cleavage, which leads to protein degradation. By maintaining a >7.0 pH environment, the Invitrogen <a href="/order/catalog/product/NP0008">NuPAGE LDS Sample Buffer</a> and <a href="/order/catalog/product/B0007">Bolt LDS Sample Buffer</a> preserve protein integrity by minimizing this Asp-Pro cleavage. As seen below, the sample integrity is maintained throughout electrophoresis with the Invitrogen NuPAGE system; protein degradation is seen in samples prepared with <i>Laemmli</i> (Tris-glycine) sample buffer. The Invitrogen <a href="/order/catalog/product/NP0005">NuPAGE Antioxidant</a> running buffer additive greatly minimizes protein oxidation during electrophoresis and keeps reduced protein bands sharp and clear.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_a669"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/_jcr_content/MainParsys/textimage_a669/image.img.jpg/1729752200140.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.full.high.jpg/1729752200053.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.full.high.jpg/1729752200053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.320.high.jpg/1729752200053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.480.high.jpg/1729752200053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.320.medium.jpg/1729752200053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.620.high.jpg/1729752200053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.320.low.jpg/1729752200053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_a669/image.img.480.medium.jpg/1729752200053.jpg" alt='NuPAGE gel system provides better sample integrity than the traditional Laemmli (tris-glycine) gel system' title='NuPAGE gel system provides better sample integrity than the traditional Laemmli (tris-glycine) gel system'></picture></div><style> #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_a669 { max-width: 100%; } #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_a669 img { width: 100%; max-width: 420px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><p><span class="icon-zoom-in"> </span>Click image to enlarge</p></small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 2. High sample integrity.</b> Integrity of samples is maintained throughout electrophoresis with the NuPAGE Bis-Tris Gel System <b>(left)</b>, compared to samples prepared with Laemmli (tris-glycine) sample buffer <b>(right)</b>.</small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><h3><br> Sharp, straight bands</h3><p>Neutral-pH buffers in NuPAGE Bis-Tris and Bolt Bis-Tris Plus Gels deliver sharp straight bands. During separation, the gels operate close to pH 7. In the Laemmli system (Tris-glycine), the gel is run at basic pH (pH ~9.5). At high pH the residual unpolymerized acrylamide can react with cysteine and lysine residues of the proteins being separated. This may affect downstream analysis such as western blot transfer. At the neutral pH of NuPAGE Bis-Tris and Bolt Bis-Tris Gels, these reactions take place several orders of magnitude more slowly than at the basic pH of Tris-glycine gels, resulting in sharper bands and better resolution.</p></div><div class="image parbase section"><div id="cq-image-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_da6"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.320.medium.jpg/1729752200253.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.480.high.jpg/1729752200253.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.620.high.jpg/1729752200253.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.full.high.jpg/1729752200253.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.320.high.jpg/1729752200253.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.480.high.jpg/1729752200253.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.320.medium.jpg/1729752200253.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.620.high.jpg/1729752200253.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.320.low.jpg/1729752200253.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/image_da6/foregroundimg.img.480.medium.jpg/1729752200253.jpg" alt='NuPAGE Bis-Tris gels provide sharp straight bands compared to competitor tris glycine gels' title='NuPAGE Bis-Tris gels provide sharp straight bands compared to competitor tris glycine gels'></picture><style> #cq-image-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_da6 img{ width:100%; max-width: 640px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_da6 .image-contain" + "," + "#cq-image-jsp-"+"319594fc-c33e-43af-86c5-2bec2ab9841bMainParsysimage_da6 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><p><small><b>Figure 3. Protein separation comparing NuPAGE Bis-Tris Gel and traditional tris-glycine gel.</b> The samples listed below were run on <b>(A)</b> a NuPAGE 4–12% Bis-Tris Gel in Invitrogen NuPAGE <a href="/order/catalog/product/NP0002">MES SDS Running Buffer</a> or <b>(B)</b> another manufacturer’s 4–20% tris-glycine gel. Note the high resolution of the sample bands in the NuPAGE protein gel. The poorly resolved “fuzzy” bands seen in the other manufacturer’s gel are a result of reoxidation of some disulfide bonds within the sample, leading to slight changes in migration rates. Lanes 1, 6, 7, 10: Invitrogen <a href="/order/catalog/product/LC5677">Mark12 Unstained Standard</a>; lane 2: high protein load (4 proteins, 6 µg/protein); lane 3: Broad Range Standard; lanes 4, 5: <i>E. coli</i> extract; lane 8: <a href="/order/catalog/product/LC5700">E-PAGE SeeBlue Pre-stained Standard</a>; lane 9: Invitrogen MultiMark Multi-Colored Protein Standard.</small></p></div><div class="heading section"><h3 id="integrity"><br>Band integrity </h3></div><div class="text parbase section"><p>NuPAGE and Bolt gels preserve protein integrity with neutral-pH gel chemistry. This formulation minimizes protein modifications during electrophoresis.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_6bdf"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/_jcr_content/MainParsys/textimage_6bdf/backgroundimg.img.jpg/1729752200598.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.320.medium.jpg/1729752200480.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.480.high.jpg/1729752200480.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.620.high.jpg/1729752200480.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.full.high.jpg/1729752200480.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.320.high.jpg/1729752200480.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.480.high.jpg/1729752200480.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.320.medium.jpg/1729752200480.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.620.high.jpg/1729752200480.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.320.low.jpg/1729752200480.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_6bdf/image.img.480.medium.jpg/1729752200480.jpg" alt='Western blot of a Bolt Bis-Tris Plus gel shows clean, sharp protein signals corresponding to full-length proteins, whereas a western blot of a Bio-Rad TGX gel shows multiple low molecular weight degradation products.' title='Western blotting results using Bolt Bis-Tris Plus gels'></picture></div><style> #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_6bdf { max-width: 100%; } #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_6bdf img { width: 100%; max-width: 650px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><p><span class="icon-zoom-in"> </span>Click image to enlarge</p></small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 4. A western blot of a Bolt Bis-Tris Plus gel shows clean, sharp protein signals corresponding to only full-length proteins, whereas a western blot of a Bio-Rad TGX gel shows multiple low molecular weight degradation products.</b> Protein kinases implicated in cancer (IKKϐ, EPHB3, HCK, MAPK14, FLT1 and DDR2) were analyzed on a Bolt Bis-Tris Plus Gel and a Bio-Rad TGX tris-glycine gel. The purified kinases (50 ng each), along with MagicMark protein standard and purified recombinant GST protein, were loaded on a 10-well, 4–12% Bolt gel and a 10-well, 4–20% Bio-Rad TGX gel. The samples were separated and transferred to 0.45-μm PVDF membranes using the respective manufacturers’ protocols. Immunodetection was performed using an anti-GST antibody and WesternBreeze chemiluminescence detection.</small></p></div></div><div class="clearfix"></div></div><div class="heading section"><h3 id="resolution"><br>Band resolution </h3></div><div class="text parbase section"><p>NuPAGE and Bolt Bis-Tris Plus Gels have better resolving power than Bio-Rad’s gels. With 10% greater resolving distance, optimized gel chemistry, and gradient format, you can see more protein bands resolved on a Bolt gel. In experimental analysis, you can see two separate bands for the insulin B chain and insulin A chain, which have similar molecular weights. The protein bands on the Bolt gel appear straight and the signal intensities are even across the entire bands, whereas Bio-Rad’s gel show fewer (<b>Figure 5C</b>), or poorly resolved <b>(Figure 5B</b>, <b>lane 2, 7 and 8</b>) protein bands.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_b374"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/_jcr_content/MainParsys/textimage_b374/backgroundimg.img.jpg/1729752200860.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.320.medium.jpg/1729752200741.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.480.high.jpg/1729752200741.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.620.high.jpg/1729752200741.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.full.high.jpg/1729752200741.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.320.high.jpg/1729752200741.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.480.high.jpg/1729752200741.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.320.medium.jpg/1729752200741.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.620.high.jpg/1729752200741.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.320.low.jpg/1729752200741.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_b374/image.img.480.medium.jpg/1729752200741.jpg" alt='Bolt Bis-Tris Gel band resolution—comparison of Bio-Rad's stained protein gels' title='Band resolution results with Bolt Bis-Tris gels'></picture></div><style> #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_b374 { max-width: 100%; } #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_b374 img { width: 100%; max-width: 640px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><p><span class="icon-zoom-in"> </span>Click image to enlarge</p></small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 5. Bolt Bis-Tris Gel band resolution—comparison of Bio-Rad's stained protein gels.</b> (A) Bolt Bis-Tris gradient gel stained with Invitrogen SimplyBlue SafeStain. (B) Bio-Rad's tris-glycine gradient gel stained with competitor’s stain. (C) Enlargement of a section of lane 5 from the two gels shows the insulin B chain (3.5 kDa) and insulin A chain (2.5 kDa).</small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p>Publication-quality protein electrophoresis data is easily generated using the higher-throughput NuPAGE WedgeWell format Bis-Tris midi gels and the <a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/surelock-tandem-midi-gel-tank.html">SureLock Tandem Midi Gel Tank</a>. The gel produces crisp bands and straight protein lanes.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_e336"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/_jcr_content/MainParsys/textimage_e336/backgroundimg.img.jpg/1729752201062.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.320.medium.jpg/1729752200942.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.480.high.jpg/1729752200942.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.620.high.jpg/1729752200942.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.full.high.jpg/1729752200942.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.320.high.jpg/1729752200942.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.480.high.jpg/1729752200942.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.320.medium.jpg/1729752200942.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.620.high.jpg/1729752200942.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.320.low.jpg/1729752200942.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_e336/image.img.480.medium.jpg/1729752200942.jpg" alt='Bolt Bis-Tris Plus gels with high sample volume capacity vs competitor gels with typical wells' title='Bolt Bis-Tris Plus gel capacity comparison'></picture></div><style> #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_e336 { max-width: 100%; } #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_e336 img { width: 100%; max-width: 650px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><p><span class="icon-zoom-in"> </span>Click image to enlarge</p></small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 6. High sample volume capacity of Bolt Bis-Tris Plus gels.</b> (A) Increasing volumes (40–70μL) of a fluorescent protein standard were loaded in every other lane of an Invitrogen Bolt 4–12% Bis-Tris Plus 10-well Gel. (B) Increasing volumes (10–40 μL) of the same protein standard were loaded in every other lane of Bio-Rad's 4–20% 10-well gel. Sample spillover and cross-well contamination is observed as signals from the sample loaded in the adjacent wells.</small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="loading"> NuPAGE Midi gels with high loading capacity wells </h2></div><div class="text parbase section"><p>WedgeWell format wells are wedge-shaped wells that double the sample loading capacity of traditional wells. WedgeWell format Midi protein gels allow the separation of up to 26 protein samples in the same run, without compromising on sample loading volume. Here are the key benefits of WedgeWell Midi gels:</p><ul><li><b>Results you can trust—</b>rigorously tested to help ensure reliable, reproducible performance, lot after lot</li><li><b>Easy sample loading—</b>reduced sample spill-over and cross-well contamination</li><li><b>Load more, see more—</b>easier detection of dilute samples and low abundance proteins</li><li><b>More data in less time—</b>obtain the information you need, faster</li><li><b>Reduced cost—</b>lower cost per sample and reduced running buffer required per sample</li><li><b>Faster run times—</b>up to 10 min faster than conventional gels</li></ul><p>NuPAGE Bis-Tris Midi WedgeWellformat gels meet the performance, reliability, and consistency standards Invitrogen precast gels are known for.</p><h4>See our <a class="cta-link" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gels-performance-guarantee.html">Protein Gel Performance Guarantee</a></h4></div><div class="heading section"><h3><br>Recommended sample loading volumes for WedgeWell format wells </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-1322862541"><tbody><tr><td width="20%" style="font-weight: bold;">Well size</td><td colspan="3" style="text-align: center; font-weight: bold;">Maximum loading volume (µL)</td></tr><tr><td>  </td><th width="30%">WedgeWell format midi gels</th><th width="30%">Standard midi gels</th></tr><tr><td>12 + 2 (small wells) </td><td>100 + 35 </td><td>50 + 15 </td></tr><tr><td>20 </td><td>60 </td><td>30 </td></tr><tr><td>26 </td><td>40 </td><td>20 </td></tr><tr><td>  </td><th>WedgeWell format mini gels</th><th>Standard mini gels</th></tr><tr><td>10 </td><td>60 </td><td>25 </td></tr><tr><td>12 </td><td>45 </td><td>20 </td></tr><tr><td>15 </td><td>35 </td><td>15 </td></tr><tr><td>17 </td><td>30 </td><td>15 </td></tr></tbody></table></div><small>*1 mm thick gel</small></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"></div></div><div style="clear:both"></div><div class="heading section"><h3> Excellent protein separation </h3></div><div class="text parbase section"><p>NuPAGE Bis-Tris Midi WedgeWell format gels offer the same exceptional protein separation of conventional NuPAGE Bis-Tris Midi gels.</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_444c"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.320.low.jpg/1729752201496.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.320.medium.jpg/1729752201496.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.480.medium.jpg/1729752201496.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.620.high.jpg/1729752201496.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.full.high.jpg/1729752201496.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.320.high.jpg/1729752201496.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.480.high.jpg/1729752201496.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.320.medium.jpg/1729752201496.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.620.high.jpg/1729752201496.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.320.low.jpg/1729752201496.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_444c/image.img.480.medium.jpg/1729752201496.jpg" alt='Coomassie stained protein gels' title='NuPAGE Bis-Tris Midi WedgeWell format and conventional NuPAGE Bis-Tris Midi gels stained with SimplyBlue Safe Stain'></picture></div><style> #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_444c { max-width: 100%; } #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_444c img { width: 100%; max-width: 900px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 7. Straight, well-resolved bands for both WedgeWell and standard midi gels.</b> Both gels were loaded as follows: Lanes 1, 20: 5 µL PageRuler Broad Range Unstained Protein Ladder (Cat. No. 26630); lanes 2–7: 10 µg, 8 µg, 6 µg, 4 µg, 2 µg, 1 µg HeLa lysate, respectively; lanes 8, 13: 5 µL Novex Mark12 Unstained Standard (Cat. No. LC5677); lanes 9–12: 240 ng, 180 ng, 120 ng, 60 ng of protein mix containing β-galactosidase, lactate dehydrogenase, and lysozyme; lanes 14–19: 10 µg, 8 µg, 6 µg, 4 µg, 2 µg, 1 µg <i>E. coli</i> lysate, respectively. Electrophoresis was conducted with NuPAGE MOPS running buffer. Gels were stained with SimplyBlue Safe Stain</small></p></div></div><div class="clearfix"></div></div><div class="heading section"><h3><br>Exceptional protein load capacity </h3></div><div class="text parbase section"><p>NuPAGE Bis-Tris Midi WedgeWell format gels offer increase sample loading capacity without compromising on the performance of the separation.</p></div><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_aff2"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2" href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/_jcr_content/MainParsys/textimage_aff2/backgroundimg.img.jpg/1729752201873.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.320.low.jpg/1729752201756.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.320.medium.jpg/1729752201756.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.480.medium.jpg/1729752201756.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.620.high.jpg/1729752201756.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.full.high.jpg/1729752201756.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.320.high.jpg/1729752201756.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.480.high.jpg/1729752201756.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.320.medium.jpg/1729752201756.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.620.high.jpg/1729752201756.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.320.low.jpg/1729752201756.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_aff2/image.img.480.medium.jpg/1729752201756.jpg" alt='Coomassie stained protein gels' title='Comparison of protein loading capacity of NuPAGE Bis-Tris and Criterion XT Bis Tris gels'></picture></div><style> #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_aff2 { max-width: 100%; } #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_aff2 img { width: 100%; max-width: 870px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><p><span class="icon-zoom-in"> </span>Click image to enlarge</p></small></div></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 8. Exceptional protein load capacity of NuPAGE Bis-Tris WedgeWell midi gels.</b> Decreasing total amount (60–0.5 µg) of HEK293 cell lysate were denatured in NUPAGE SDS Sample Buffer, loaded in a NuPAGE Bis-Tris Wedgewell 4–12% 12+2 Well Midi Gel and run in the SureLock Tandem Midi Gel Tank. Equal amounts of HEK293 cell lysate were denatured in XT sample buffer, loaded in a Bio-Rad 4–12% XT12+2 well midi gel and run in the Criterion Cell Midi Gel Tank.</small></p></div></div><div class="clearfix"></div></div><div class="heading section"><h3> Comparison of electrophoresis and western blotting of cell lysates using NuPAGE midi gels versus Bio-Rad </h3></div><div class="text parbase section"><p>The protein load capacities of Invitrogen NuPAGE Bis-Tris precast midi gels run in the SureLock Tandem Mid Gel Tank were compared against Bio-Rad Criterion midi gels run in a Bio-Rad Criterion Cell Midi Cell Tank using manufacturer instructions. Decreasing amounts of HEK 293 cell lysate prepared in RIPA lysis buffer (48–0.5 µg total protein) were denatured in the respective manufacturer’s sample buffer and subjected to electrophoresis using manufacturer instructions. The table below lists the samples, protein mass, and %RIPA buffer loaded in each lane.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1237856372"><tbody><tr><th style="width: 20%;">Lane</th><th>+1</th><th>1</th><th>2</th><th>3</th><th>4</th><th>5</th><th>6</th><th>7</th><th>8</th><th>9</th><th>10</th><th valign="top">11</th><th valign="top">12</th><th valign="top">+1</th></tr><tr><th>Sample</th><td>Sample buffer</td><td>iBright Protein Ladder</td><td colspan="10" style="text-align: center;">HEK293 lysate</td><td>iBright Protein Ladder</td><td>Sample buffer</td></tr><tr><th>Load mass (µg)</th><td>-</td><td>-</td><td>48</td><td>40</td><td>32</td><td>24</td><td>16</td><td>8</td><td>4</td><td>2</td><td>1</td><td>0.5</td><td>-</td><td>-</td></tr><tr><th>Load vol. (µL)</th><td>3</td><td>3</td><td>20</td><td>20</td><td>20</td><td>20</td><td>20</td><td>20</td><td>20</td><td>20</td><td>20</td><td>20</td><td>3</td><td>3</td></tr><tr><th>% RIPA buffer</th><td>-</td><td>-</td><td style="text-align: justify;"><b>40</b></td><td style="text-align: justify;"><b>33.3</b></td><td style="text-align: justify;"><b>26.7</b></td><td style="text-align: justify;"><b>20</b></td><td style="text-align: justify;"><b>13.3</b></td><td style="text-align: justify;"><b>6.7</b></td><td style="text-align: justify;"><b>3.3</b></td><td style="text-align: justify;"><b>1.7</b></td><td style="text-align: justify;"><b>0.8</b></td><td style="text-align: justify;"><b>0.4</b></td><td>-</td><td>-</td></tr></tbody></table></div></div><div class="text parbase section"><p>Invitrogen NuPAGE Bis-Tris gels outperformed Bio-Rad gels at higher lysate loads with blots from Bio-Rad gels showing band loss and smearing at higher loads for all targets investigated.</p></div><div class="heading section"><h3><br>Comparison of protein blotting and western detection of cell lysates </h3></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_56a2"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.320.low.jpg/1729752202112.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.320.medium.jpg/1729752202112.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.480.medium.jpg/1729752202112.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.620.high.jpg/1729752202112.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.full.high.jpg/1729752202112.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.full.high.jpg/1729752202112.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.320.high.jpg/1729752202112.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.480.high.jpg/1729752202112.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.320.medium.jpg/1729752202112.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.620.high.jpg/1729752202112.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.320.low.jpg/1729752202112.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/textimage_56a2/image.img.480.medium.jpg/1729752202112.jpg" alt='NuPAGE gel system provides better sample integrity than the traditional Laemmli (tris-glycine) gel system' title='TGX-midi-gels-western-blot-results'></picture></div><style> #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_56a2 { max-width: 100%; } #cq-textimage-jsp-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystextimage_56a2 img { width: 100%; max-width: 3309px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 9: Western blots using NuPAGE 4-12% Bis-Tris midi gels generate sharper bands at greater protein and RIPA lysis buffer loads than Bio-Rad 4–20% TGX midi gels. </b>A NuPAGE 4–12% Bis-Tris midi gel, 12+2 well, was loaded with decreasing total protein amount of HEK293 lysate, subjected to electrophoresis in a SureLock Tandem Midi Gel Tank and transferred onto a 0.45 µm PVDF membrane using the SureLock Tandem Blot Module and the Bio-Rad 4–20% TGX midi gel, 12+2 well, was subjected to electrophoresis in a Criterion Midi Cell Tank and transferred onto a 0.45 µm PVDF membrane using the Criterion Blotter. Both membranes were probed for three targets (vinculin, α-tubulin, and p23) using fluorescent immunodetection. Band loss and smearing can be seen at the higher loads for all targets on the Bio-Rad blot, while the blot for the NuPAGE Bis-Tris gel offers superior protein loading capacity above 24 µg and crisp, bright bands.<br> For immunodetection: membranes were blocked for 1 hour in 1X <a adhocenable="false" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-buffers.html">Blocker FL Fluorescent Blocking Buffer</a>. For fluorescence: multiplex detection, the membranes were probed overnight with a mixture of primary antibodies diluted in blocking solution: <a adhocenable="false" href="/antibody/product/Vinculin-Antibody-clone-42H89L44-Recombinant-Monoclonal/700062?imageId=706667">Rabbit-anti Vinculin</a> (1:15,000), <a adhocenable="false" href="/antibody/product/alpha-Tubulin-Antibody-clone-YL1-2-Monoclonal/MA1-80017">Rat anti-α Tubulin</a> (1:5,000)), and <a adhocenable="false" href="/antibody/product/alpha-Tubulin-Antibody-clone-YL1-2-Monoclonal/MA1-80017">Mouse-anti p23</a> (1:5,000) followed by an incubation with secondary antibodies diluted at 1:5,000 in 1X Blocker FL: <a adhocenable="false" href="/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A32802">Donkey anti-Rabbit Alexa Fluor Plus 680, Donkey anti-Rat Alexa Fluor 488</a>, and Donkey anti-Mouse Alexa Fluor Plus 800 for 1 hour. Membranes were imaged on an <a adhocenable="false" href="/sg/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-imaging-analysis/ibright-systems.html">iBright Imaging System</a>, with the same time and image adjustment settings for each blot.</small></p></div></div><div class="clearfix"></div></div><div class="text parbase section"><p style="text-align: right;"><a href="#top"><b>TOP</b></a></p></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="order"> Ordering Information </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-28fc21404171551">NuPAGE Bis-Tris Midi Gels</a></li><li><a data-toggle="tab" href="#container-28fc21404171552">Bolt Bis-Tris Plus Mini Gels</a></li><li><a data-toggle="tab" href="#container-28fc21404171553">NuPAGE Bis-Tris Mini Gels</a></li><li><a data-toggle="tab" href="#container-28fc21404171554">Reagents</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-28fc21404171551" class="tab-pane active"><div class="container-28fc21404171551 tabstep"><div class="start"></div><div class="tab parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">WBT41212BOX,WBT41220BOX,WBT41226BOX,WBT01012BOX,WBT01020BOX,WBT01026BOX,WG1201BOX,WG1202BOX,WG1203BOX,WG1401BOX,WG1402BOX,WG1403BOX,WG1001BOX,WG1002BOX,WG1003BOX,WG1201A,WG1202A,WG1203A,WG1401A,WG1402A,WG1403A,WG1001A,WG1002A,WG1003A </div><div id='ltajax-8558443801026076809'></div><script type="text/javascript">(function() {$('#ltajax-8558443801026076809').load('/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/tabworkflowcontainer_2140417155/container-28fc21404171551/tab/productlist_b7a2.ajax.html?skulist=');})(); 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</script></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystabworkflowcontainer_2140417155" href="#collapse2"><span class="caret"></span><strong> Bolt Bis-Tris Plus Mini Gels </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-28fc21404171552"><div class="container-28fc21404171552 tabstep"><div class="start"></div><div class="tab parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">NW04120BOX,NW04122BOX,NW04125BOX,NW04127BOX,NW00100BOX,NW00102BOX,NW00105BOX,NW00107BOX,NW00120BOX,NW00122BOX,NW00125BOX,NW00127BOX,NW00080BOX,NW00082BOX,NW00085BOX,NW00087BOX</div><div id='ltajax-5680719753086522954'></div><script type="text/javascript">(function() {$('#ltajax-5680719753086522954').load('/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/tabworkflowcontainer_2140417155/container-28fc21404171552/tab/productlist_3858.ajax.html?skulist=');})(); </script></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystabworkflowcontainer_2140417155" href="#collapse3"><span class="caret"></span><strong> NuPAGE Bis-Tris Mini Gels </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-28fc21404171553"><div class="container-28fc21404171553 tabstep"><div class="start"></div><div class="tab parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">NP0321BOX,NP0322BOX,NP0323BOX,NP0329BOX,NP0327BOX,NP0335BOX,NP0336BOX,NP0324BOX,NP0301BOX,NP0302BOX,NP0303BOX,NP0307BOX,NP0315BOX,NP0316BOX,NP0341BOX,NP0342BOX,NP0343BOX,NP0349BOX</div><div id='ltajax-5900631028031489837'></div><script type="text/javascript">(function() {$('#ltajax-5900631028031489837').load('/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/tabworkflowcontainer_2140417155/container-28fc21404171553/tab/productlist_e96d.ajax.html?skulist=');})(); </script></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-319594fc-c33e-43af-86c5-2bec2ab9841bMainParsystabworkflowcontainer_2140417155" href="#collapse4"><span class="caret"></span><strong> Reagents </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-28fc21404171554"><div class="container-28fc21404171554 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3 id="reagents"> NuPAGE Bis-Tris and Bolt Bis-Tris Plus Reagents </h3></div><div class="productlist parbase section"><div id="skulist" style="display:none">NP0007,NP0008,NP0002,NP0060,NP0001,NP0050,NP0005,NP0004,NP0009,NP0006,NW2000,A25977,B1000,NP0007,NP0008,NP0002,NP0060,NP0001,NP0050,NP0005,NP0004,NP0009,NP0006,NW2000,A25977,B1000</div><div id='ltajax-6826093629380319274'></div><script type="text/javascript">(function() {$('#ltajax-6826093629380319274').load('/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/nupage-bis-tris-gels/jcr:content/MainParsys/tabworkflowcontainer_2140417155/container-28fc21404171554/tab/productlist_3e7c.ajax.html?skulist=');})(); </script></div></div><div class="end"></div></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well well-white well-mini"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2" id="related-resources"> Related resources </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_5af"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-selection-guide.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.full.high.png/1731006187968.png' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.full.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.320.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.480.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.320.medium.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.620.high.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.320.low.png/1731006187968.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_5af/foregroundimg.img.480.medium.png/1731006187968.png" alt='Protein Gel Selection Guide' title='Protein Gel Selection Guide'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_5af .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_5af .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A2"><div class="text parbase section"><p><b><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-selection-guide.html">Protein Gel Selection Guide</a></b></p><p>Find an excellent mini or midi gel for your protein electrophoresis experiment.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_e09d"><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-conversion-tool.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.full.high.jpg/1731006188006.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.full.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.320.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.480.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.320.medium.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.620.high.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.320.low.jpg/1731006188006.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_e09d/foregroundimg.img.480.medium.jpg/1731006188006.jpg" alt='Gel Selection Chart' title='Gel Selection Chart'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_e09d .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_e09d .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B2"><div class="text parbase section"><p><b><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gel-conversion-tool.html">Protein Gel Conversion Tool</a></b></p><p>Find an exceptional Invitrogen mini or midi gel to replace your current precast gel from another supplier.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_2a38"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target='_blank'><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.full.high.jpg/1731006188053.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.full.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.320.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.480.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.320.medium.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.620.high.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.320.low.jpg/1731006188053.jpg,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_beda/uipar/image_2a38/foregroundimg.img.480.medium.jpg/1731006188053.jpg" alt='Protein Gel Options Brochure' title='Protein Gel Options Brochure'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_2a38 .image-contain" + "," + "#cq-image-jsp-"+"d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_bedauiparimage_2a38 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="C2"><div class="text parbase section"><p><b><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target="_blank">Gel Migration Chart</a></b></p><p>Download the Protein Gel Guide for a printable gel migration chart.</p></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h4>Still unsure which precast gel works best for your research needs? Download the <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/precast-gel-selection-guide.pdf" target="_blank">Gel Selection Chart</a></h4></div></div></div></div></div></div></div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><h3>Manuals</h3><ul class="the-icons"><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/nupage_tech_man.pdf" target="_blank">NuPAGE Technical Guide</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0007891_NuPAGE_BisTris_MiniGels.pdf" target="_blank">NuPAGE Bis-Tris Gel Mini Gel Protocol</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN1000410-NuPAGEBisTrisWedgeWellMidiGels-QR.pdf" target="_blank">NuPAGE Bis-Tris Gel Midi Gel Protocol</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0007902_Bolt_BisTrisPlus_MiniGels_QR.pdf" target="_blank">Bolt Bis-Tris Plus Mini Gel Protocol</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mini_gel_tank_man.pdf" target="_blank">Mini Gel Tank Manual</a></li><li><span class="icon-lt-pdf"></span> <a href="/content/dam/LifeTech/Documents/PDFs/MAN0018507_SureLockMidiGelTank_UG.pdf" target="_blank">SureLock Tandem Midi Gel Tank USER GUIDE</a></li><li><span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/xcell4_midicell_man.pdf" target="_blank">XCell4 SureLock Manual</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><h3>Handbooks</h3><ul><li><a href="/sg/en/home/global/forms/protein-gel-electrophoresis-western-blotting-handbooks.html">Protein Gel Electrophoresis Handbook</a></li><li><a href="/sg/en/home/global/forms/protein-gel-electrophoresis-western-blotting-handbooks.html">Protein Transfer Technical Handbook</a></li></ul></div></div></div></div><div style="clear:both"></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_7440uipartextimage_797c"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.full.high.png/1731006188138.png' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.full.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.320.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.480.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.320.medium.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.620.high.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.320.low.png/1731006188138.png,https://www.thermofisher.com/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/jcr:content/MainParsys/well_7440/uipar/textimage_797c/image.img.480.medium.png/1731006188138.png" alt='Protein Gels Performance Guarantee' title='Protein Gels Performance Guarantee'></picture></div><style> #cq-textimage-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_7440uipartextimage_797c { max-width: 100%; } #cq-textimage-jsp-d71cba74-5c7c-4bac-b897-88a2cf652f65MainParsyswell_7440uipartextimage_797c img { width: 100%; max-width: 263px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3>Protein Gels Performance Guarantee</h3><p>We stand behind the performance of our protein gels.</p><p><a href="/sg/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gels/protein-gels-performance-guarantee.html" class="cta-link">Learn more</a></p></div></div><div class="clearfix"></div></div></div></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Resources </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt4"><div class="parsys_column cq-colctrl-lt4-c0"><div container-id="cq-colctrl-lt4" comp-id="A1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_4e94"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.full.high.jpg/1706109796911.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.full.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.320.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.480.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.320.medium.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.620.high.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.320.low.jpg/1706109796911.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_4e94/image.img.480.medium.jpg/1706109796911.jpg" alt='Protein Gel Guide' title='Protein Gel Guide'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_4e94 { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_4e94 img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Download: <a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/precast-protein-gels-brochure.pdf" target="_blank">Protein Gel Guide</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c1"><div container-id="cq-colctrl-lt4" comp-id="B1"><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_55c0"><a href="/sg/en/home/global/forms/protein-gel-electrophoresis-western-blotting-handbooks.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.full.high.jpg/1706109797029.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.full.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.320.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.480.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.320.medium.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.620.high.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.320.low.jpg/1706109797029.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_55c0/image.img.480.medium.jpg/1706109797029.jpg" alt='Protein gel electrophoresis technical handbook' title='Protein gel electrophoresis technical handbook'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_55c0 { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_55c0 img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Download: <a href="/sg/en/home/global/forms/protein-gel-electrophoresis-western-blotting-handbooks.html" target="_blank">Protein gel electrophoresis technical handbook</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c2"><div container-id="cq-colctrl-lt4" comp-id="C1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_183d"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/western-workflow-overview-brochure.pdf" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.full.high.jpg/1706109797055.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.full.high.jpg/1706109797055.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.320.high.jpg/1706109797055.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.480.high.jpg/1706109797055.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.320.medium.jpg/1706109797055.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.620.high.jpg/1706109797055.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.320.low.jpg/1706109797055.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_183d/image.img.480.medium.jpg/1706109797055.jpg" alt='Western Detection Workflow Brochure' title='Western Detection Workflow Brochure'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_183d { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_183d img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Download: <a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/western-workflow-overview-brochure.pdf" target="_blank">Western Detection Workflow brochure</a></p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt4-c3"><div container-id="cq-colctrl-lt4" comp-id="D1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_6ecc"><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-gel-electrophoresis-information.html" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.full.high.jpg/1706109797082.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.full.high.jpg/1706109797082.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.320.high.jpg/1706109797082.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.480.high.jpg/1706109797082.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.320.medium.jpg/1706109797082.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.620.high.jpg/1706109797082.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.320.low.jpg/1706109797082.jpg,https://www.thermofisher.com/sg/en/reference-components/protein-biology-reference-components/jcr:content/MainParsys/well_64c4/uipar/textimage_6ecc/image.img.480.medium.jpg/1706109797082.jpg" alt='Protocols, tips &amp; tricks and electrophoresis troubleshooting' title='Protocols, tips &amp; tricks and electrophoresis troubleshooting'></picture></div><style> #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_6ecc { max-width: 100%; } #cq-textimage-jsp-1127b475-b65b-4840-ba2d-40bb8bba6f5cMainParsyswell_64c4uipartextimage_6ecc img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>Access: <a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-gel-electrophoresis-information.html">Protocols, tips & tricks, and electrophoresis troubleshooting</a></p></div></div><div class="clearfix"></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_d1d9 LTRawHTML"><style> /* Remove horizontal scroll on hero banner table */ .wellPageHeading .well-container .table-component-wrapper table {width:100% !important;} .content.wide-template .container-wrap.template-2-column .span3.sidebar > .container-leftnav {margin-right:20px;} /*.railsection {padding-right:10px;}*/ .textimage {margin-bottom:0px;} /* Label display for author */ .ux-tool-label { display: none; 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