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Eastabrook</title> <meta name="description" content="Search results for: Rowan Eastabrook"> <meta name="keywords" content="Rowan Eastabrook"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Rowan Eastabrook"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 8</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Rowan Eastabrook</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Bacteriological Analysis of Logan's Branch Rowan County, Kentucky Utilizing Membrane Filtration Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20G.%20Hereford">Elizabeth G. Hereford</a>, <a href="https://publications.waset.org/abstracts/search?q=Geoffrey%20W.%20Gearner"> Geoffrey W. Gearner</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Logan’s Branch, within the Triplett Creek Watershed of Rowan County, Kentucky, is a waterway located near important agricultural and residential areas. Part of Logan’s Branch flows over an exposed black shale formation with elevated radioactivity and heavy metals. Three sites were chosen in relation to the formation and sampled five times over a thirty-day period during the recreational season. A fourth site in North Fork in Rowan County, Kentucky was also sampled periodically as it too has contact with the shale formation. These sites were then sampled monthly. All samples are analyzed for concentrations of Escherichia coli, heterotrophic bacteria, and total coliform bacteria utilizing the membrane filtration method and various culture media. Current data suggests that the radioactivity of the shale formation influences the bacteriological growth present in the waterway; however, further data will be collected and compared with that of my colleagues to confirm this trend. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteriological%20analysis" title="bacteriological analysis">bacteriological analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=Escherichia%20coli" title=" Escherichia coli"> Escherichia coli</a>, <a href="https://publications.waset.org/abstracts/search?q=heterotrophic%20bacteria" title=" heterotrophic bacteria"> heterotrophic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=radioactive%20black%20shale%20formation" title=" radioactive black shale formation"> radioactive black shale formation</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20quality" title=" water quality"> water quality</a> </p> <a href="https://publications.waset.org/abstracts/86253/bacteriological-analysis-of-logans-branch-rowan-county-kentucky-utilizing-membrane-filtration-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86253.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">188</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Risk Factors for Acute Respiratory Infection Among Children Under Five in Tanzania: A Systematic Review and Analysis of the 2015 Demographic and Health Survey for Tanzania</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ayesha%20Ali">Ayesha Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Emilia%20Lindquist"> Emilia Lindquist</a>, <a href="https://publications.waset.org/abstracts/search?q=Arif%20Jalal"> Arif Jalal</a>, <a href="https://publications.waset.org/abstracts/search?q=Hannah%20Yusuf"> Hannah Yusuf</a>, <a href="https://publications.waset.org/abstracts/search?q=Kayan%20Cheung"> Kayan Cheung</a>, <a href="https://publications.waset.org/abstracts/search?q=Rowan%20Eastabrook">Rowan Eastabrook</a> </p> <p class="card-text"><strong>Abstract:</strong></p> It is currently estimated that over a third of deaths in children under five in Tanzania are caused by acute respiratory infections (ARIs). However, despite being one of the leading causes of morbidity and mortality across the developing world, its risk factors are poorly understood. Therefore, a systematic review of the literature published between 2015 and 2020 was conducted, focusing on risk factors for ARI in Tanzanian children under the age of five. 2015 Demographic and Health Survey (DHS) for Tanzania was analysed to supplement these findings with national data. 2224 papers were retrieved from two databases and were analysed by three independent reviewers. Thirteen papers were eligible for inclusion, covering a wide range of risk factors among which comorbidities (n=6), malnutrition (n=5), lack of parental education (n=4), poor socio-economic status (n=3), and delay in seeking healthcare (n=3) were the most cited risk factors. The risk factors with the highest reported risk ratios/odds ratios were lack of parental education (RR=11.5-14.5), followed by enrolment in school (RR=4.4), delay in seeking healthcare (RR=3.8) and cooking indoors (aOR =1.8-RR=5.5). The DHS data provided local context to these risk factors. For instance, the number of children experiencing symptoms of ARI in both urban and rural areas ranged between 4.5-5% in the two weeks prior to the survey. However, 79% of symptomatic children in Zanzibar received antibiotics for treatment compared to just 34% of those in the Southern Highlands. As demonstrated by both the systematic review and the DHS analysis, risk factors for ARI are predominantly socially determined, with Tanzania’s poorer rural children possessing the highest risk for ARI and more adverse health outcomes. Therefore, the burden of ARIs in Tanzanian children may be alleviated through the provision of appropriate treatment and parental education in rural areas. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acute%20respiratory%20infection" title="acute respiratory infection">acute respiratory infection</a>, <a href="https://publications.waset.org/abstracts/search?q=child" title=" child"> child</a>, <a href="https://publications.waset.org/abstracts/search?q=health%20education" title=" health education"> health education</a>, <a href="https://publications.waset.org/abstracts/search?q=morbidity" title=" morbidity"> morbidity</a>, <a href="https://publications.waset.org/abstracts/search?q=mortality" title=" mortality"> mortality</a>, <a href="https://publications.waset.org/abstracts/search?q=pneumonia" title=" pneumonia"> pneumonia</a>, <a href="https://publications.waset.org/abstracts/search?q=Tanzania" title=" Tanzania"> Tanzania</a> </p> <a href="https://publications.waset.org/abstracts/139596/risk-factors-for-acute-respiratory-infection-among-children-under-five-in-tanzania-a-systematic-review-and-analysis-of-the-2015-demographic-and-health-survey-for-tanzania" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139596.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">192</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Exploring the Situational Approach to Decision Making: User eConsent on a Health Social Network</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=W.%20Rowan">W. Rowan</a>, <a href="https://publications.waset.org/abstracts/search?q=Y.%20O%E2%80%99Connor"> Y. O’Connor</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Lynch"> L. Lynch</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Heavin"> C. Heavin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Situation Awareness can offer the potential for conscious dynamic reflection. In an era of online health data sharing, it is becoming increasingly important that users of health social networks (HSNs) have the information necessary to make informed decisions as part of the registration process and in the provision of eConsent. This research aims to leverage an adapted Situation Awareness (SA) model to explore users’ decision making processes in the provision of eConsent. A HSN platform was used to investigate these behaviours. A mixed methods approach was taken. This involved the observation of registration behaviours followed by a questionnaire and focus group/s. Early results suggest that users are apt to automatically accept eConsent, and only later consider the long-term implications of sharing their personal health information. Further steps are required to continue developing knowledge and understanding of this important eConsent process. The next step in this research will be to develop a set of guidelines for the improved presentation of eConsent on the HSN platform<em>.</em> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=eConsent" title="eConsent">eConsent</a>, <a href="https://publications.waset.org/abstracts/search?q=health%20social%20network" title=" health social network"> health social network</a>, <a href="https://publications.waset.org/abstracts/search?q=mixed%20methods" title=" mixed methods"> mixed methods</a>, <a href="https://publications.waset.org/abstracts/search?q=situation%20awareness" title=" situation awareness"> situation awareness</a> </p> <a href="https://publications.waset.org/abstracts/74829/exploring-the-situational-approach-to-decision-making-user-econsent-on-a-health-social-network" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74829.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">292</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Ultra-Fast pH-Gradient Ion Exchange Chromatography for the Separation of Monoclonal Antibody Charge Variants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Robert%20van%20Ling">Robert van Ling</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexander%20Schwahn"> Alexander Schwahn</a>, <a href="https://publications.waset.org/abstracts/search?q=Shanhua%20Lin"> Shanhua Lin</a>, <a href="https://publications.waset.org/abstracts/search?q=Ken%20Cook"> Ken Cook</a>, <a href="https://publications.waset.org/abstracts/search?q=Frank%20Steiner"> Frank Steiner</a>, <a href="https://publications.waset.org/abstracts/search?q=Rowan%20Moore"> Rowan Moore</a>, <a href="https://publications.waset.org/abstracts/search?q=Mauro%20de%20Pra"> Mauro de Pra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: Demonstration of fast high resolution charge variant analysis for monoclonal antibody (mAb) therapeutics within 5 minutes. Methods: Three commercially available mAbs were used for all experiments. The charge variants of therapeutic mAbs (Bevacizumab, Cetuximab, Infliximab, and Trastuzumab) are analyzed on a strong cation exchange column with a linear pH gradient separation method. The linear gradient from pH 5.6 to pH 10.2 is generated over time by running a linear pump gradient from 100% Thermo Scientific™ CX-1 pH Gradient Buffer A (pH 5.6) to 100% CX-1 pH Gradient Buffer B (pH 10.2), using the Thermo Scientific™ Vanquish™ UHPLC system. Results: The pH gradient method is generally applicable to monoclonal antibody charge variant analysis. In conjunction with state-of-the-art column and UHPLC technology, ultra fast high-resolution separations are consistently achieved in under 5 minutes for all mAbs analyzed. Conclusion: The linear pH gradient method is a platform method for mAb charge variant analysis. The linear pH gradient method can be easily optimized to improve separations and shorten cycle times. Ultra-fast charge variant separation is facilitated with UHPLC that complements, and in some instances outperforms CE approaches in terms of both resolution and throughput. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=charge%20variants" title="charge variants">charge variants</a>, <a href="https://publications.waset.org/abstracts/search?q=ion%20exchange%20chromatography" title=" ion exchange chromatography"> ion exchange chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=monoclonal%20antibody" title=" monoclonal antibody"> monoclonal antibody</a>, <a href="https://publications.waset.org/abstracts/search?q=UHPLC" title=" UHPLC"> UHPLC</a> </p> <a href="https://publications.waset.org/abstracts/63884/ultra-fast-ph-gradient-ion-exchange-chromatography-for-the-separation-of-monoclonal-antibody-charge-variants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63884.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">440</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Baby Boomers and Millennials: Creating a Specialized Orientation Program</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Rowan">K. Rowan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, the author will discuss how developing a specialized orientation has improved nursing satisfaction and decrease the incidence of incivility among staff. With the predicted shortages in nursing, we must provide an environment that reflects the needs of the current workforce while also focusing on the sustainability of nursing. Each generation has different qualities and methods in which he or she prefers to learn. The Baby Boomer has a desire to share their knowledge. They feel that the quality of undergraduate nursing education has declined. Millennials have grown up with 'helicopter parents' and expect the preceptor to behave in the same manner. This information must be shared with the Baby Boomer, as it is these staff members who are passing the torch of perioperative nursing. Currently, nurse fellows are trained with the Association of periOperative Nurse’s Periop 101 program, with a didactic and clinical observation program. There is no specialized perioperative preceptor program. In creation of a preceptor program, the concept of Novice to Expert, communication techniques, dealing with horizontal violence and generational gap education is reviewed with the preceptor. The fellows are taught communication and de-escalation skills, and generational gaps information. The groups are then brought together for introductions and teamwork exercises. At the program’s core is the knowledge of generational differences. The preceptor training has increased preceptor satisfaction, as well as the new nurse fellows. The creation of a specialized education program has significantly decreased incivility amongst our nurses, all while increasing nursing satisfaction and improving nursing retention. This model of program can translate to all nursing specialties and assist in overcoming the impending shortage. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=baby%20boomers" title="baby boomers">baby boomers</a>, <a href="https://publications.waset.org/abstracts/search?q=education" title=" education"> education</a>, <a href="https://publications.waset.org/abstracts/search?q=generational%20gap" title=" generational gap"> generational gap</a>, <a href="https://publications.waset.org/abstracts/search?q=millennials" title=" millennials"> millennials</a>, <a href="https://publications.waset.org/abstracts/search?q=nursing" title=" nursing"> nursing</a>, <a href="https://publications.waset.org/abstracts/search?q=perioperative" title=" perioperative"> perioperative</a> </p> <a href="https://publications.waset.org/abstracts/89980/baby-boomers-and-millennials-creating-a-specialized-orientation-program" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/89980.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">166</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Depth-Averaged Modelling of Erosion and Sediment Transport in Free-Surface Flows</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Rowan">Thomas Rowan</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20Seaid"> Mohammed Seaid</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A fast finite volume solver for multi-layered shallow water flows with mass exchange and an erodible bed is developed. This enables the user to solve a number of complex sediment-based problems including (but not limited to), dam-break over an erodible bed, recirculation currents and bed evolution as well as levy and dyke failure. This research develops methodologies crucial to the under-standing of multi-sediment fluvial mechanics and waterway design. In this model mass exchange between the layers is allowed and, in contrast to previous models, sediment and fluid are able to transfer between layers. In the current study we use a two-step finite volume method to avoid the solution of the Riemann problem. Entrainment and deposition rates are calculated for the first time in a model of this nature. In the first step the governing equations are rewritten in a non-conservative form and the intermediate solutions are calculated using the method of characteristics. In the second stage, the numerical fluxes are reconstructed in conservative form and are used to calculate a solution that satisfies the conservation property. This method is found to be considerably faster than other comparative finite volume methods, it also exhibits good shock capturing. For most entrainment and deposition equations a bed level concentration factor is used. This leads to inaccuracies in both near bed level concentration and total scour. To account for diffusion, as no vertical velocities are calculated, a capacity limited diffusion coefficient is used. The additional advantage of this multilayer approach is that there is a variation (from single layer models) in bottom layer fluid velocity: this dramatically reduces erosion, which is often overestimated in simulations of this nature using single layer flows. The model is used to simulate a standard dam break. In the dam break simulation, as expected, the number of fluid layers utilised creates variation in the resultant bed profile, with more layers offering a higher deviation in fluid velocity . These results showed a marked variation in erosion profiles from standard models. The overall the model provides new insight into the problems presented at minimal computational cost. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=erosion" title="erosion">erosion</a>, <a href="https://publications.waset.org/abstracts/search?q=finite%20volume%20method" title=" finite volume method"> finite volume method</a>, <a href="https://publications.waset.org/abstracts/search?q=sediment%20transport" title=" sediment transport"> sediment transport</a>, <a href="https://publications.waset.org/abstracts/search?q=shallow%20water%20equations" title=" shallow water equations"> shallow water equations</a> </p> <a href="https://publications.waset.org/abstracts/73381/depth-averaged-modelling-of-erosion-and-sediment-transport-in-free-surface-flows" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/73381.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">217</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Quantitative, Preservative Methodology for Review of Interview Transcripts Using Natural Language Processing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rowan%20P.%20Martnishn">Rowan P. Martnishn</a> </p> <p class="card-text"><strong>Abstract:</strong></p> During the execution of a National Endowment of the Arts grant, approximately 55 interviews were collected from professionals across various fields. These interviews were used to create deliverables – historical connections for creations that began as art and evolved entirely into computing technology. With dozens of hours’ worth of transcripts to be analyzed by qualitative coders, a quantitative methodology was created to sift through the documents. The initial step was to both clean and format all the data. First, a basic spelling and grammar check was applied, as well as a Python script for normalized formatting which used an open-source grammatical formatter to make the data as coherent as possible. 10 documents were randomly selected to manually review, where words often incorrectly translated during the transcription were recorded and replaced throughout all other documents. Then, to remove all banter and side comments, the transcripts were spliced into paragraphs (separated by change in speaker) and all paragraphs with less than 300 characters were removed. Secondly, a keyword extractor, a form of natural language processing where significant words in a document are selected, was run on each paragraph for all interviews. Every proper noun was put into a data structure corresponding to that respective interview. From there, a Bidirectional and Auto-Regressive Transformer (B.A.R.T.) summary model was then applied to each paragraph that included any of the proper nouns selected from the interview. At this stage the information to review had been sent from about 60 hours’ worth of data to 20. The data was further processed through light, manual observation – any summaries which proved to fit the criteria of the proposed deliverable were selected, as well their locations within the document. This narrowed that data down to about 5 hours’ worth of processing. The qualitative researchers were then able to find 8 more connections in addition to our previous 4, exceeding our minimum quota of 3 to satisfy the grant. Major findings of the study and subsequent curation of this methodology raised a conceptual finding crucial to working with qualitative data of this magnitude. In the use of artificial intelligence there is a general trade off in a model between breadth of knowledge and specificity. If the model has too much knowledge, the user risks leaving out important data (too general). If the tool is too specific, it has not seen enough data to be useful. Thus, this methodology proposes a solution to this tradeoff. The data is never altered outside of grammatical and spelling checks. Instead, the important information is marked, creating an indicator of where the significant data is without compromising the purity of it. Secondly, the data is chunked into smaller paragraphs, giving specificity, and then cross-referenced with the keywords (allowing generalization over the whole document). This way, no data is harmed, and qualitative experts can go over the raw data instead of using highly manipulated results. Given the success in deliverable creation as well as the circumvention of this tradeoff, this methodology should stand as a model for synthesizing qualitative data while maintaining its original form. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=B.A.R.T.model" title="B.A.R.T.model">B.A.R.T.model</a>, <a href="https://publications.waset.org/abstracts/search?q=keyword%20extractor" title=" keyword extractor"> keyword extractor</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20language%20processing" title=" natural language processing"> natural language processing</a>, <a href="https://publications.waset.org/abstracts/search?q=qualitative%20coding" title=" qualitative coding"> qualitative coding</a> </p> <a href="https://publications.waset.org/abstracts/189003/quantitative-preservative-methodology-for-review-of-interview-transcripts-using-natural-language-processing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/189003.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">31</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Full Characterization of Heterogeneous Antibody Samples under Denaturing and Native Conditions on a Hybrid Quadrupole-Orbitrap Mass Spectrometer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rowan%20Moore">Rowan Moore</a>, <a href="https://publications.waset.org/abstracts/search?q=Kai%20Scheffler"> Kai Scheffler</a>, <a href="https://publications.waset.org/abstracts/search?q=Eugen%20Damoc"> Eugen Damoc</a>, <a href="https://publications.waset.org/abstracts/search?q=Jennifer%20Sutton"> Jennifer Sutton</a>, <a href="https://publications.waset.org/abstracts/search?q=Aaron%20Bailey"> Aaron Bailey</a>, <a href="https://publications.waset.org/abstracts/search?q=Stephane%20Houel"> Stephane Houel</a>, <a href="https://publications.waset.org/abstracts/search?q=Simon%20Cubbon"> Simon Cubbon</a>, <a href="https://publications.waset.org/abstracts/search?q=Jonathan%20Josephs"> Jonathan Josephs</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: MS analysis of monoclonal antibodies (mAbs) at the protein and peptide levels is critical during development and production of biopharmaceuticals. The compositions of current generation therapeutic proteins are often complex due to various modifications which may affect efficacy. Intact proteins analyzed by MS are detected in higher charge states that also provide more complexity in mass spectra. Protein analysis in native or native-like conditions with zero or minimal organic solvent and neutral or weakly acidic pH decreases charge state value resulting in mAb detection at higher m/z ranges with more spatial resolution. Methods: Three commercially available mAbs were used for all experiments. Intact proteins were desalted online using size exclusion chromatography (SEC) or reversed phase chromatography coupled on-line with a mass spectrometer. For streamlined use of the LC- MS platform we used a single SEC column and alternately selected specific mobile phases to perform separations in either denaturing or native-like conditions: buffer A (20 % ACN, 0.1 % FA) with Buffer B (100 mM ammonium acetate). For peptide analysis mAbs were proteolytically digested with and without prior reduction and alkylation. The mass spectrometer used for all experiments was a commercially available Thermo Scientific™ hybrid Quadrupole-Orbitrap™ mass spectrometer, equipped with the new BioPharma option which includes a new High Mass Range (HMR) mode that allows for improved high mass transmission and mass detection up to 8000 m/z. Results: We have analyzed the profiles of three mAbs under reducing and native conditions by direct infusion with offline desalting and with on-line desalting via size exclusion and reversed phase type columns. The presence of high salt under denaturing conditions was found to influence the observed charge state envelope and impact mass accuracy after spectral deconvolution. The significantly lower charge states observed under native conditions improves the spatial resolution of protein signals and has significant benefits for the analysis of antibody mixtures, e.g. lysine variants, degradants or sequence variants. This type of analysis requires the detection of masses beyond the standard mass range ranging up to 6000 m/z requiring the extended capabilities available in the new HMR mode. We have compared each antibody sample that was analyzed individually with mixtures in various relative concentrations. For this type of analysis, we observed that apparent native structures persist and ESI is benefited by the addition of low amounts of acetonitrile and formic acid in combination with the ammonium acetate-buffered mobile phase. For analyses on the peptide level we analyzed reduced/alkylated, and non-reduced proteolytic digests of the individual antibodies separated via reversed phase chromatography aiming to retrieve as much information as possible regarding sequence coverage, disulfide bridges, post-translational modifications such as various glycans, sequence variants, and their relative quantification. All data acquired were submitted to a single software package for analysis aiming to obtain a complete picture of the molecules analyzed. Here we demonstrate the capabilities of the mass spectrometer to fully characterize homogeneous and heterogeneous therapeutic proteins on one single platform. Conclusion: Full characterization of heterogeneous intact protein mixtures by improved mass separation on a quadrupole-Orbitrap™ mass spectrometer with extended capabilities has been demonstrated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=disulfide%20bond%20analysis" title="disulfide bond analysis">disulfide bond analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=intact%20analysis" title=" intact analysis"> intact analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=native%20analysis" title=" native analysis"> native analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=monoclonal%20antibodies" title=" monoclonal antibodies"> monoclonal antibodies</a>, <a href="https://publications.waset.org/abstracts/search?q=peptide%20mapping" title=" peptide mapping"> peptide mapping</a>, <a href="https://publications.waset.org/abstracts/search?q=post-translational%20modifications" title=" post-translational modifications"> post-translational modifications</a>, <a href="https://publications.waset.org/abstracts/search?q=sequence%20variants" title=" sequence variants"> sequence variants</a>, <a href="https://publications.waset.org/abstracts/search?q=size%20exclusion%20chromatography" title=" size exclusion chromatography"> size exclusion chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=therapeutic%20protein%20analysis" title=" therapeutic protein analysis"> therapeutic protein analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=UHPLC" title=" UHPLC"> UHPLC</a> </p> <a href="https://publications.waset.org/abstracts/63438/full-characterization-of-heterogeneous-antibody-samples-under-denaturing-and-native-conditions-on-a-hybrid-quadrupole-orbitrap-mass-spectrometer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63438.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">362</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> 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