SARS-CoV-2 Spike S1 Subunit Antibody (MAB105805) by R&D Systems, Part of Bio-Techne

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class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13391">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" 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Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" 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href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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data-time_to_build="0.02001690864563" data-time_datapoints="0.81326508522034" data-time_template="-0.81326508522034" data-time_post_epcc="0.1943678855896" data-time_epcc="0.81326508522034" data-render_time="Wednesday 30th of October 2024 03:48:21 PM" data-fallback="FALSE" data-time_to_process_data="0.22229290008545" class="px-4 container" class="container"> <div id="above-fold-section"> <div class="mb-2 o__product-summary"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> SARS-CoV-2 Spike S1 Subunit Antibody </h1> <h2 class="font-weight-normal h4"> R&D Systems, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">MAB105805</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="submit_review_wrapper" class="col-auto d-flex align-self-center"> <div id="submit_review"> <a href="/reviews/MAB105805" class="submit_review" rel="nofollow"> <span class="icon-plus-circle d-none d-lg-inline pr-1"></span> Submit a review </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <div class="px-0 my-1" id="datasheet-summary-wrapper" data-section="section-base--top-jumplink"> <article class="top-jumplink-wrapper"> <span class="icon-icon-pdf text-error-red"></span> <a href="#product-documents" class="product-documents-top-link" rel="nofollow">Product Datasheet / COA / SDS</a> </article> </div> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> <div class="row border-top mb-2" id="images-orderdetail-wrapper"> <div class="col-xl-6 mt-2" id="images-wrapper" data-section="image_summary"> <div id="image-tree" data-section="component-product-image-tree" class="vertical lightbox" data-mdb-zoom-effect="true"> <div id="product-image-gallery" class="row"> <div class="col-md-2 d-none d-md-block"> <ul class="list-inline m-0"> <li data-id="1" class="product-image-lightbox border rounded mb-2 list-inline-item"> <div class="text-center mt-1"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-1212021164447.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-1212021164447.jpg" alt="" title="Detection of SARS-CoV-2 Spike S1 protein bound to ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry." data-caption="HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was incubated with Recombinant SARS-CoV-2 Spike S1 Subunit His-Tag protein (<a href="/p/proteins-enzymes/recombinant-sars-cov-2-spike-s1-subunit-his-tag-protein-cf_10522-cv" target="_self">10522-CV</a>), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (<a href="/p/isotype-controls/mouse-igg-2a-isotype-control_mab003" target="_self">MAB003</a>) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). Staining was performed using our Staining Membrane-associated Proteins protocol." data-badges='' data-mdb-img="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-1212021164447.jpg" data-id="0" data-application="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-1212021164447.jpg" class="lazy" /> </div> </li> </ul> </div> <div class="col-md-10 border rounded d-flex align-items-center justify-content-center product-image-lightbox"> <div class="text-center my-2" data-brand="rnd"> <img src="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-222022124748.jpg" alt="" title="Detection of SARS-CoV-2 Omicron protein (B.1.1.529 variant) bound to ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry." data-caption="HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was incubated with Recombinant SARS-CoV-2 Omicron His-Tag protein (B.1.1.529 variant; <a href="/p/proteins-enzymes/recombinant-sars-cov-2-b11529-s-rbd-his-tag-protein-cf_11056-cv" target="_self">11056-CV</a>), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (<a href="/p/isotype-controls/mouse-igg-2a-isotype-control_mab003" target="_self">MAB003</a>) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). Staining was performed using our Staining Membrane-associated Proteins protocol." data-badges='' data-id="0" width="250" height="250" fetchpriority="high" as="image" class="search_result_thumbnail_image active" /> </div> </div> </div> </div> </div> <div class="col-xl-6 mt-2" id="orderdetails-wrapper" data-section="order_detail_block"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="MAB105805"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 p-0 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-3 p-0 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-2 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="MAB105805-100" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">MAB105805-100</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="MAB105805-SP" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">MAB105805-SP</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <div class="atc-control ProductCart-control text-right"> <div class="row container d-flex justify-content-end mx-0 mb-3 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100">Print Quote</a> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB105805" class="atc-bulk-button btn btn-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> <div class="atc-container order_detail_wrapper conjugates-and-formulations-wrapper my-2 position-relative"> <div class="button-group text-right pl-2"> <a href="#conjugates-MAB105805" class="btn btn-secondary atc-view-pricing atc-view-pricing-products collapsed text-nowrap mb-2" data-id="conjugates-MAB105805" data-toggle="collapse" aria-expanded="false" aria-controls="conjugates">View Available Conjugates </a> </div> <div id="conjugates-MAB105805" class="conjugates-and-formulations-container collapse position-absolute w-100"> <div class="atc-container border rounded bg-white shadow p-2 mt-1"> <div class="commerce-atc-datasheet atc-block-collapsible" data-type="conjugates_and_formulations" data-blockid="mab105805-conjugate-atc-block"> <div id="mab105805-conjugate-atc-block" class="atc-lines"> <div class="atc-header row pb-1 m-0"> <div class="atc-head col-4 p-0 font-weight-bold d-sm-none d-md-block">Conjugate</div> <div class="atc-head col-4 p-0 font-weight-bold">Catalog #</div> <div class="atc-head col-4"></div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805U" data-titleoverwrite="Alexa Fluor 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-350-conjugated-antibody-1035226_fab105805u">Alexa Fluor 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-350-conjugated-antibody-1035226_fab105805u">FAB105805U</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-350-conjugated-antibody-1035226_fab105805u" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805V" data-titleoverwrite="Alexa Fluor 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-405-conjugated-antibody-1035226_fab105805v">Alexa Fluor 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-405-conjugated-antibody-1035226_fab105805v">FAB105805V</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-405-conjugated-antibody-1035226_fab105805v" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805G" data-titleoverwrite="Alexa Fluor 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-488-conjugated-antibody-1035226_fab105805g">Alexa Fluor 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-488-conjugated-antibody-1035226_fab105805g">FAB105805G</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-488-conjugated-antibody-1035226_fab105805g" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805X" data-titleoverwrite="Alexa Fluor 532"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805x">Alexa Fluor 532</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805x">FAB105805X</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805x" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805T" data-titleoverwrite="Alexa Fluor 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-594-conjugated-antibody-1035226_fab105805t">Alexa Fluor 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-594-conjugated-antibody-1035226_fab105805t">FAB105805T</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-594-conjugated-antibody-1035226_fab105805t" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805R" data-titleoverwrite="Alexa Fluor 647"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-647-conjugated-antibody-1035226_fab105805r">Alexa Fluor 647</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-647-conjugated-antibody-1035226_fab105805r">FAB105805R</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-647-conjugated-antibody-1035226_fab105805r" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805N" data-titleoverwrite="Alexa Fluor 700"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-700-conjugated-antibody-1035226_fab105805n">Alexa Fluor 700</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-700-conjugated-antibody-1035226_fab105805n">FAB105805N</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-700-conjugated-antibody-1035226_fab105805n" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805S" data-titleoverwrite="Alexa Fluor 750"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-750-conjugated-antibody-1035226_fab105805s">Alexa Fluor 750</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-750-conjugated-antibody-1035226_fab105805s">FAB105805S</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-subunit-alexa-fluor-750-conjugated-antibody-1035226_fab105805s" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805A" data-titleoverwrite="Allophycocyanin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805a">Allophycocyanin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805a">FAB105805A</a> </div> </div> </div> <div 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button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805B" data-titleoverwrite="Biotin"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805b">Biotin</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805b">FAB105805B</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805b" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805CL1" data-titleoverwrite="CoraFluor 1"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805cl1">CoraFluor 1</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805cl1">FAB105805CL1</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805cl1" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805D" data-titleoverwrite="DyLight 350"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805d">DyLight 350</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805d">FAB105805D</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805d" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805E" data-titleoverwrite="DyLight 405"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805e">DyLight 405</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805e">FAB105805E</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805e" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805K" data-titleoverwrite="DyLight 488"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805k">DyLight 488</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805k">FAB105805K</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805k" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805L" data-titleoverwrite="DyLight 550"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805l">DyLight 550</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805l">FAB105805L</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805l" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805M" data-titleoverwrite="DyLight 594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805m">DyLight 594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805m">FAB105805M</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805m" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805W" data-titleoverwrite="DyLight 650"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805w">DyLight 650</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805w">FAB105805W</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805w" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805Y" data-titleoverwrite="DyLight 680"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805y">DyLight 680</a> </div> <div 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View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805H" data-titleoverwrite="HRP"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805h">HRP</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805h">FAB105805H</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805h" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805JF525" data-titleoverwrite="Janelia Fluor 525"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805jf525">Janelia Fluor 525</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805jf525">FAB105805JF525</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805jf525" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805I" data-titleoverwrite="Janelia Fluor 549"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a 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p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805jf635" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805J" data-titleoverwrite="Janelia Fluor 646"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805j">Janelia Fluor 646</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805j">FAB105805J</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805j" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805JF669" data-titleoverwrite="Janelia Fluor 669"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805jf669">Janelia Fluor 669</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805jf669">FAB105805JF669</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805jf669" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805MFV450" data-titleoverwrite="mFluor Violet 450 SE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805mfv450">mFluor Violet 450 SE</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805mfv450">FAB105805MFV450</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805mfv450" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805MFV500" data-titleoverwrite="mFluor Violet 500 SE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum 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</div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805mfv610">FAB105805MFV610</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805mfv610" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805P" data-titleoverwrite="PE"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805p">PE</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805p">FAB105805P</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805p" class="btn button--primary col-xs-4 order-last btn-md-block w-100 p-1"> View Product </a> </div> </div> </div> <div class="commerce-atc-conjugates-formulations-productline mt-2 border-top" data-catalog="FAB105805PEATT594" data-titleoverwrite="PE/Atto594"> <div class="row my-2 mx-0"> <div class="col-8 my-auto"> <div class="row"> <div class="commerce-atc-catnum col-12 col-md-6 p-0 font-weight-bold"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805peatt594">PE/Atto594</a> </div> <div class="commerce-atc-price col-12 col-md-6 p-0"> <a href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805peatt594">FAB105805PEATT594</a> </div> </div> </div> <div class="commerce-atc-atc-btn col-4 p-0"> <a type="button" href="/p/antibodies/sars-cov-2-spike-s1-protein-antibody-1035226_fab105805peatt594" class="btn button--primary 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Storage</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#background"> Background</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#product-documents"> Product Documents</a> </li> <li class="nav-item m-0"> <a class="nav-link d-inline jump-anchor " href="#specific-notices"> Specific Notices</a> </li> </ul> </div> <div id="key_details" class="py-5 bt-pp-border-bottom" data-section="section-base--key-details"> <h3>Key Product Details</h3> <div class="mt-5 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Species Reactivity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <div class="d-block pb-2"> SARS-CoV-2 </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Applications</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <div class="d-block pb-2"> CyTOF-ready, Flow Cytometry </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Label</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Unconjugated </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Antibody Source</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal Mouse IgG<sub>2A</sub> Clone # 1035226 </div> </div> </div> <div class="container"> <div class="row product-data"> <div class="col-lg-6 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4><a href="/t/spike-s1-subunit/antibodies">View all Spike S1 Subunit Antibodies »</a></h4> </div> </div> </div> </div> <div class="border-top border-bottom recommended-products-section" id="recommendation-viewed" data-product_code="MAB105805" data-type=viewed></div> <div id="product-specifications" class="bt-pp-border-bottom py-5" data-section="section-base--product-specifications"> <h3>Product Specifications</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Immunogen</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> HEK293-derived SARS-CoV-2 Spike S1 Subunit<br />Accession # YP_009724390.1 </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Specificity</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Detects SARS-CoV-2 Spike S1 and SARS-CoV-2 B.1.1.529 S RBD (Omicron Variant) in direct ELISAs. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Clonality</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Monoclonal </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Host</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Mouse </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Isotype</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> IgG<sub>2A</sub> </div> </div> </div> </div> <div id="scientific-data" class="bt-pp-border-bottom py-5" data-anchor-label="Scientific Data" data-section="section-base--scientific-data"> <div> <h3>Scientific Data Images for SARS-CoV-2 Spike S1 Subunit Antibody </h3> </div> <div class="product-data-wrapper mt-5" id="scientific-data-table" data-scientific-count="-1"> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-222022124748.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-222022124748.jpg" alt="" title="Detection of SARS-CoV-2 Omicron protein (B.1.1.529 variant) bound to ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry." data-caption="HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was incubated with Recombinant SARS-CoV-2 Omicron His-Tag protein (B.1.1.529 variant; <a href="/p/proteins-enzymes/recombinant-sars-cov-2-b11529-s-rbd-his-tag-protein-cf_11056-cv" target="_self">11056-CV</a>), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (<a href="/p/isotype-controls/mouse-igg-2a-isotype-control_mab003" target="_self">MAB003</a>) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). Staining was performed using our Staining Membrane-associated Proteins protocol." data-badges="" data-application="Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Detection of SARS-CoV-2 Omicron protein (B.1.1.529 variant) bound to ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry.</h4> HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was incubated with Recombinant SARS-CoV-2 Omicron His-Tag protein (B.1.1.529 variant; <a href="/p/proteins-enzymes/recombinant-sars-cov-2-b11529-s-rbd-his-tag-protein-cf_11056-cv" target="_self">11056-CV</a>), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (<a href="/p/isotype-controls/mouse-igg-2a-isotype-control_mab003" target="_self">MAB003</a>) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). Staining was performed using our Staining Membrane-associated Proteins protocol. </div> </div> </div> <div> <div class="row m___product-data-examples my-4" data-section="product-image-divs"> <div class="col-md-2 border-0 mb-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-1212021164447.jpg" data-srcset="https://resources.bio-techne.com/images/products/antibody/mab105805_sars-cov-2-spike-rbd-mab-clone-1035226-flow-cytometry-1212021164447.jpg" alt="" title="Detection of SARS-CoV-2 Spike S1 protein bound to ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry." data-caption="HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was incubated with Recombinant SARS-CoV-2 Spike S1 Subunit His-Tag protein (<a href="/p/proteins-enzymes/recombinant-sars-cov-2-spike-s1-subunit-his-tag-protein-cf_10522-cv" target="_self">10522-CV</a>), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (<a href="/p/isotype-controls/mouse-igg-2a-isotype-control_mab003" target="_self">MAB003</a>) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). Staining was performed using our Staining Membrane-associated Proteins protocol." data-badges="" data-application="Flow Cytometry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> <div class="col-md-9 border-0 align-top"> <h4 class="font-weight-bold">Detection of SARS-CoV-2 Spike S1 protein bound to ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry.</h4> HEK293 human embryonic kidney cell line transfected with human ACE-2 and eGFP was incubated with Recombinant SARS-CoV-2 Spike S1 Subunit His-Tag protein (<a href="/p/proteins-enzymes/recombinant-sars-cov-2-spike-s1-subunit-his-tag-protein-cf_10522-cv" target="_self">10522-CV</a>), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (<a href="/p/isotype-controls/mouse-igg-2a-isotype-control_mab003" target="_self">MAB003</a>) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a href="/p/secondary-antibodies/mouse-f-ab-2-igg-h-l-apc-conjugated-antibody_f0101b" target="_self">F0101B</a>). Staining was performed using our Staining Membrane-associated Proteins protocol. </div> </div> </div> <span class="product-data-wrapper table" id="more-scientific-data" style="display:none"> </span> </div> </div> <div id="applications" class="py-5 bt-pp-border-bottom" data-anchor-label="Applications" data-section="section-antibody--applications"> <h3>Applications for SARS-CoV-2 Spike S1 Subunit Antibody </h3> <div class="mt-5 py-2 container"> <div class="row"> <div class="col-lg-3 border font-weight-bold text-bg-dark py-2 align-top text-md-left text-white bg-dark-bt-blue d-none d-lg-block"> Application </div> <div class="col-lg-9 border align-top py-2 font-weight-bold text-white bg-dark-bt-blue d-none d-lg-block"> Recommended Usage </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>CyTOF-ready</h4> </div> <div class="col-lg-9 border align-top py-2"> Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. </div> </div> <div class="row"> <div class="col-lg-3 border font-weight-bold product-data-label py-2 align-top text-md-left text-break"> <h4>Flow Cytometry</h4> </div> <div class="col-lg-9 border align-top py-2"> 0.25 µg/10<sup>6</sup> cells <br/>Sample: SARS-CoV-2 Spike S1 protein (Catalog # 10522-CV)  and SARS-CoV-2 B.1.1.529 S RBD (Catalog 11056-CV) bound to ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP. </div> </div> </div> <div class="product-data-wrapper mt-5"> Please Note: Optimal dilutions of this antibody should be experimentally determined. </div> </div> <div id="preparation--storage" class="bt-pp-border-bottom py-5" data-section="section-base--preparation-storage"> <h3>Formulation, Preparation, and Storage</h3> <div class="mt-4 py-2 container"> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Purification</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Protein A or G purified from hybridoma culture supernatant </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Reconstitution</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <div class="atc-container"> <div class="commerce-atc-reconstitution atc-block" data-type="reconstitution" data-blockid="ReconstitutionCart"> <div class="commerce-atc-reconstitution__instructions"> Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration. </div> <p class="atc-modal"></p> <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode=""> <div class="commerce-atc-reconstitution-header atc-header col-four row border-top pt-2 m-0"> <div class="commerce-atc-catnum atc-head col-md-6 font-weight-bold p-0">Reconstitution Buffer Available:</div> <div class="commerce-atc-price atc-head col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-md-3 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ReconstitutionCart" class="atc-lines"> <div class="commerce-atc-reconstitution-line productline col-four row py-2 m-0" data-catalog="RB01"> <div class="commerce-atc-title col-md-6 p-0"> <a href="">RB01: </a> </div> <div class="commerce-atc-price col-md-3"> </div> <div class="commerce-atc-quantity col-md-3 text-right p-0"> </div> </div> </div> <div class="atc-control ReconstitutionCart-control text-right mt-2"> <input type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc col-xs-4"/> </div> </div> </form> </div> </div> </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Formulation</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Shipping</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below. </div> </div> <div class="row product-data"> <div class="col-lg-3 font-weight-bold product-data-label py-2 align-top text-bt-dark-blue"> <h4>Stability & Storage</h4> </div> <div class="col-lg-9 product-data-value py-2 align-top text-break"> <b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL> </div> </div> </div> </div> <div id="background" class="py-5 bt-pp-border-bottom" data-section="section-base--background-info"> <h3>Background: Spike S1 Subunit</h3> <article class="product-data-wrapper"> SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). Based on structural biology studies, the receptor binding domain (RBD), located in the C-terminal region of S1, can be oriented either in the up/standing or down/lying state (6). The standing state is associated with higher pathogenicity and both SARS-CoV-1 and MERS can access this state due to the flexibility in their respective RBDs. A similar two-state structure and flexibility is found in the SARS-CoV-2 RBD (7). Based on amino acid (aa) sequence homology, the SARS-CoV-2  S1 subunit has 65% identity with SARS-CoV-1 S1 subunit,  but only 22% homology with the MERS S1 subunit. The low aa sequence homology is consistent with the finding that SARS and MERS bind different cellular receptors (8). The S Protein of the SARS-CoV-2 virus, like the SARS-CoV-1 counterpart, binds Angiotensin-Converting Enzyme 2 (ACE2), but with much higher affinity and faster binding kinetics (9). Before binding to the ACE2 receptor, structural analysis of the S1 trimer shows that only one of the three RBD domains in the trimeric structure is in the "up" conformation. This is an unstable and transient state that passes between trimeric subunits but is nevertheless an exposed state to be targeted for neutralizing antibody therapy (10). Polyclonal antibodies to the RBD of the SARS-CoV-2 S1 subunit have been shown to inhibit interaction with the ACE2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy (11). There is also promising work showing that the RBD may be used to detect presence of neutralizing antibodies present in a patient's bloodstream, consistent with developed immunity after exposure to the SARS-CoV-2 virus (12). Lastly, it has been demonstrated the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (13, 14). </article> <div id="background_wrapper" class="px-3 mt-2"> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>References</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <ol><li>Wu, F. <em>et al</em>. (2020) Nature <strong>579</strong>:265.</li><li>Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. <strong>105</strong>:93.</li><li>Bosch, B.J. <em>et al</em>. (2003) J. Virol. <strong>77</strong>:8801.</li><li>Belouzard, S. <em>et al</em>. (2009) Proc. Natl. Acad. Sci. <strong>106</strong>:5871.</li><li>Millet, J.K. and G. R. Whittaker (2015) Virus Res. <strong>202</strong>:120.</li><li>Yuan, Y. <em>et al</em>. (2017) Nat. Commun. <strong>8</strong>:15092.</li><li>Walls, A.C. <em>et al</em>. (2010) Cell <strong>180</strong>:281.</li><li>Jiang, S. <em>et al</em>. (2020) Trends. Immunol. <a href="https://doi.org/10.1016/j.it.2020.03.007">https://doi.org/10.1016/j.it.2020.03.007</a>.</li><li>Ortega, J.T. <em>et al</em>. (2020) EXCLI J. <strong>19</strong>:410.</li><li>Wrapp, D. <em>et al</em>. (2020) Science <strong>367</strong>:1260.</li><li>Tai, W. <em>et al</em>. (2020) Cell. Mol. Immunol. <a href="https://doi.org/10.1016/j.it.2020.03.007">https://doi.org/10.1016/j.it.2020.03.007</a>.</li><li>Okba, N. M. A. <em>et al</em>. (2020). Emerg. Infect. Dis. <a href="https://doi.org/10.3201/eid2607.200841">https://doi.org/10.3201/eid2607.200841</a>. </li><li>Wang, X. <em>et al</em>. (2020) <a href="https://doi.org/10.1038/s41423-020-0424-9">https://doi.org/10.1038/s41423-020-0424-9</a>.</li><li>Wang, K. <em>et al</em>. (2020) bioRxiv <a href="https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1">https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1</a>. </li></ol> </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Long Name</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> Spike Protein, S1 Subunit </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>Alternate Names</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> SARS-CoV-2 </div> </div> <div class="row product-data"> <div class=" col-md-3 product-data-label font-weight-bold p-3 align-top border"> <h4>UniProt</h4> </div> <div class=" col-md-9 product-data-value p-3 px-4 text-break border"> <a href="https://www.uniprot.org/uniprot/?query=YP_009724390.1" class="a___link-external" target="_blank" rel="nofollow">YP_009724390.1</a> </div> </div> </div> <h4 class="mt-3">Additional Spike S1 Subunit Products</h4> <ul class="list-unstyled"> <li><a href="/t/spike-s1-subunit" data-product="Spike S1 Subunit">All Products for Spike S1 Subunit </a></li> <li><a href="/t/spike-s1-subunit/antibodies" data-application="Spike S1 Subunit Primary Antibodies">Spike S1 Subunit Primary Antibodies </a></li> <li><a href="/t/spike-s1-subunit/proteins-enzymes" data-application="Spike S1 Subunit Proteins and Enzymes">Spike S1 Subunit Proteins and Enzymes </a></li> </ul> </div> <div id="product-documents" data-section="section-base--product-documents"> <div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3"> Product Documents for SARS-CoV-2 Spike S1 Subunit Antibody </h3> </div> <div class="vtab-section" data-section="Product Datasheets"> <div class="card-header rounded-top"> <a class="" data-toggle="collapse" data-target="#collapse_two" aria-expanded="true" 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SARS-CoV-2 Spike S1 Subunit Antibody (MAB105805) by R&D Systems, Part of Bio-Techne