Modified Oligos | Oligo Modifications

<!DOCTYPE html>    <html lang="en">  <head> <title>Modified Oligos | Oligo Modifications | GENEWIZ from Azenta</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="Modified Oligos services are designed to equip researchers with customized oligos featuring a wide variety of modifications."> <meta name="keywords" content="oligo, oligo modifications, modified oligos, modified oligo, modified oligonucleotide, oligo modification, genewiz, azenta, azenta life sciences"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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Choose your synthetic scale, HPLC or PAGE purification options, and delivery format without paying premium prices. Spend more time innovating and less time worrying about your budget, with custom oligo solutions you can depend on.</p> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/OlgCustom?lobId=4&serviceId=411" target="_blank"><button>Place an Order</button></a>  <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Resources/FAQs/FAQs-Oligo" target="_blank"><button>Oligo FAQ</button></a> </div> <div class="dsp-content-box" id="dsp-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02Bw8pq0" target="_blank"> <img alt="" src="/-/media/14006-D_Modified-Oligo-Order_WebBanRight-240612.ashx?la=en&hash=A9BAE8114BDA4BAAC447273F0C79D6C95B36F736" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; 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}); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Modified Oligo Applications</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Therapeutic Development:</strong> Interrogate gene function and control gene expression. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Fundamental Research:</strong> Perform hybridization and antisense experiments, cloning/PCR, immunoassays, and control polymerase/ligase/nuclease activity. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Gene Editing: </strong>Create (small guide) sgRNA for use in CRISPR/Cas9 systems. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Molecular Diagnostics:</strong> Prepare DNA microarrays, nucleic acid probes, and functional handles for the attachment of custom modifiers. </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .epi-service-options { width: 1170px; margin: 0 auto; text-align: center; margin-top: 60px; } .service-container { width: 100%; text-align: center; display:flex; justify-content: space-around; flex-wrap: wrap; } .epi-service { width: 32%; text-align:center; /*display: inline-block;*/ /*vertical-align: top;*/ padding: 20px 0 75px 0; margin: 4px; position: relative; } .epi-service img { height: 110px; } .epi-service h3 { margin: 0; } .epi-service p { width: 90%; margin: 0 auto; /*margin-bottom: 20px;*/ line-height: 20px; } .epi-service a { color: #3a3a3a; } #epi-service-1 { background: #ffffff; } #epi-service-1 span { display: none; } #epi-service-2 { background: #ffffff; } #epi-service-3 { background: #ffffff; } .epi-service-btn { background: #826ec3; border: 2px solid #826ec3; color: #fff; padding: 10px 20px; -webkit-transition: background-color 0.2s ease-in-out; -moz-transition: background-color 0.2s ease-in-out; -o-transition: background-color 0.2s ease-in-out; transition: background-color 0.2s ease-in-out; position: absolute; bottom: 20px; right:25%; left:37%; width:55%; margin-left: -55px; margin-top: 30px; } .epi-service-btn:hover { background: #826ec3; border: 2px solid #826ec3; color: #fff; padding: 10px 20px; } @media only screen and (max-width: 1170px) { .epi-service-options { width: 90%; } .epi-service-btn { } } @media only screen and (max-width: 905px) { .epi-service { width: 80%; } } @media only screen and (max-width: 768px) { .service-container { display: block; } .epi-service { width: 100%; margin: 10px 0; padding: 10px 0 20px 0; } .epi-service-btn { position: static; left: 0; right: 0; margin: 0; } .epi-service p { margin-bottom: 20px; } } </style> <div class="epi-service-options" id="epigenomics-service-options"> <h2>Oligo Modification Services</h2> <div class="service-container"> <div class="epi-service" id="epi-service-1"> <div class="epi-service-content"> <img alt="" src="/-/media/Images/DNA-Oligo-Modifications.ashx?la=en&hash=7FB8EC918685F65F760E4E7751EB636C3084A57B"> <h3><strong>DNA Oligo Modifications</strong></h3> <br> <p>With your choice of over 200 chemical modifications, our standard oligo synthesis services provide the high-quality results you expect from Azenta with the breadth of oligo synthesis expertise you need for your smaller DNA synthesis experiments.</p> </div> <a href="#oligo"><button class="epi-service-btn">Find the Right Oligo</button></a> </div> <div class="epi-service" id="epi-service-2"> <div class="epi-service-content"> <img alt="" src="/-/media/Images/Custom-Oligo-Projects.ashx?la=en&hash=85D39A0073B487DEC823BAE471FCB1DAB7437756"> <h3><strong>Custom Oligo Projects</strong></h3> <br> <p>Azenta offers nearly any oligo solution. Mixed bases, NGS adapter primers, oligo pools, DEL libraries, special delivery scales/formats, and more are accessible through our custom online ordering form to meet your exact project requirements.</p> <p> </p> <p> </p> </div> <a rel="noopener noreferrer" href="https://clims4.genewiz.com/" target="_blank"><button class="epi-service-btn">Place a Custom Order</button></a> </div> <div class="epi-service" id="epi-service-3"> <div class="epi-service-content"> <img alt="" src="/-/media/Images/RNA-Oligos.ashx?la=en&hash=342601B757E04FC2817FBEAD1A0A84E964623303"> <h3><strong>RNA Oligos</strong></h3> <br> <p>Synthetically designed RNA is used in research applications such as gene function analysis and therapeutic development. Azenta’s expertise in oligonucleotide synthesis ensures your RNA, modified RNA, and RNA/DNA chimeras are <br> delivered quickly and in high-quality.</p> </div> <a href="https://www.genewiz.com/en/Public/Services/Oligo/Custom-RNA-Oligos"><button class="epi-service-btn">Learn More</button></a> </div> <div class="epi-service" id="epi-service-4"> <div class="epi-service-content"> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; padding: 0; background: #FFFFFF; } .data-container { margin: 0 auto; padding-top: 30px; background: #FFFFFF; } .data { margin: 0 auto; text-align: center; width: 1170px; } .data-step { display: inline-block; vertical-align: top; width: 19%; padding: 10px; margin-bottom: 20px; position: relative; } .data-step img { width: 150px; margin: 0 auto; margin-bottom: 20px; } .data-step h3 { text-align: center; font-size: 18px; font-family: sans-serif; border-bottom: none; } .data-step p { text-align: center; font-size: 12px; font-family: sans-serif; border-bottom: none; } .data-icon { text-align: center; } .ngs-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 17px; letter-spacing: 1px; width:50%; margin-left:25%; margin-right:25%; } .ngs-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 1170px) { .data { text-align: left; margin: 0 auto; width: 100%; } .data-step { width: 90%; } } @media only screen and (max-width: 500px) { .data-step { width: 100%; } } </style> <div class="data-container"> <div class="container"> <h2 style="text-align: center; margin-bottom: 30px;" id="oligo">Featured Application: Gene Editing</h2> <p style="text-align: center;">RNA oligos are used to control transcription, translation, and a host of other cellular activities. Because of their central role in molecular biology, RNA can function as important therapeutics and probes for genetic function. Additionally, they are critical components of gene editing programs to provide templates for CRISPR/Cas9 endonuclease activity. Complete single guide RNA (sgRNA) constructs from Azenta enable gene knock-out/knock-in experiments without the need for an extra annealing step between crRNA and tracrRNA subunits.</p> <div class="data-step"> <div class="data-icon"> <h3><strong>snoRNA</strong></h3> <img alt="" src="/-/media/Images/snoRNA.ashx?la=en&hash=FCD872619BA109562CFF2B824076B70EE349E5D4" /> </div> <p><strong>Facilitates chemical modification of RNAs</strong></p> </div> <div class="data-step"> <div class="data-icon"> <h3><strong>miRNA</strong></h3> <img alt="" src="/-/media/Images/miRNA.ashx?la=en&hash=DF0FEBAC906C59F54682AA70D73B078700D48602" /> </div> <p><strong>Regulates gene expression</strong></p> </div> <div class="data-step"> <div class="data-icon"> <h3><strong>siRNA</strong></h3> <img alt="" src="/-/media/Images/siRNA.ashx?la=en&hash=AD70FF79394CA32358797F8DCC7A750C88AE446D" /> </div> <p><strong>Silences gene expression</strong></p> </div> <div class="data-step"> <div class="data-icon"> <h3><strong>snRNA</strong></h3> <img alt="" src="/-/media/Images/snRNA.ashx?la=en&hash=6C0597DBC28B8536DDC512A85930104226843392" /> </div> <p><strong>Functions in various nuclear processes (e.g. splicing)</strong></p> </div> <div class="data-step"> <div class="data-icon"> <h3><strong>sgRNA</strong></h3> <img alt="" src="/-/media/Images/sgRNA.ashx?la=en&hash=509A205BFDB89AD0BDE4AF59E07B740B2F36D0EF" /> </div> <p><strong>Edit genes with CRISPR/Cas9 systems</strong></p> </div> <p style="text-align:center;">Discover the library of RNA structures and modifications available from Azenta.</p> </div> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Oligo/Custom-RNA-Oligos" target="_blank"><button>Learn More</button></a> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> * { box-sizing: border-box; } .column1 { float: left; width: 60%; padding: 10px; } .column2 { float: left; width: 40%; padding: 10px; } .row:after { content: ""; display: table; clear: both; } .Libraries-content-box { display: inline-flex; vertical-align: left; } #Libraries-content-box-left { width: 50%; text-align: left; padding: 30px 0; } #Libraries-content-box-right { width: 30%; text-align: center; } .Libraries-content-box img { width: 450px; height: 110px; float: center; margin-top: 30px; } .Libraries-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 10px; letter-spacing: 1px; width:20%; margin-left:40%; margin-right:40%; } .Libraries-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 1170px) { .Libraries-content-box { max-width: 100%; } .Libraries-content-box img { max-width: 450px; max-height: 110px; float: left; } } @media only screen and (max-width: 768px) { .Libraries-content-box { display: block; width: 100%; } </style> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <h2 style="text-align: center;">Popular Oligo Modifications</h2> <p>Below are just a few examples of common DNA oligo synthesis services and modifications ordered by Azenta customers. Contact our Ph.D. technical support team for a comprehensive list of over 200 modifications, more information, or guidance on custom synthetic needs for any research project in your lab.</p> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#FFFFFF;"> <h3 style="text-align: center;">Attachment modifications</h3> <p style="text-align: left;">Attachment modifications offer benefits to many fundamental research and molecular diagnostic applications. Biotinylation and digoxigenination are functional in immunoassays (protein detection; ELISA), hybridization (Southern blotting; Northern blotting; in situ), cellular staining, and pull-down isolation experiments. Aminolinkers and thiols both provide functional handles for further attachments either internally or at 5’ or 3’ positions.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/Oligo-flex-1.ashx?la=en&hash=01FF3C18C2873C523D71198CD06CE8A829F19509" /> </div> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#FFFFFF;"> <h3 style="text-align: center;">Quencher Modification</h3> <p style="text-align: left;">Quenchers are used in conjunction with a fluorophore to investigate DNA and/or RNA interactions. These systems are typically part of FRET or reporter-quencher experiments.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/Oligo-flex-4-New.ashx?la=en&hash=1801B96E9D3B91B979A98775E201E5CD4E838596" /> </div> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#FFFFFF;"> <h3 style="text-align: center;">Fluorescent Dye Modification</h3> <p style="text-align: left;">Dyes are generally used to localize DNA and/or RNA sequences of interest in the cell. Choose from a variety of fluorescent dyes with a wide spectrum of absorption, emission, and color depending on the needs of your experiment and detection technique.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/Oligo-flex-3.ashx?la=en&hash=77C9AE71E8CB6C02F77AFA2EE8A126B6EC0807B5" /> </div> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#FFFFFF;"> <h3 style="text-align: center;">Modified Bases</h3> <p style="text-align: left;">Modified nucleosides such as deoxyinosine (dI) provide degeneracy in DNA oligos with limited sequence information, while deoxyuridine (dU) improves double stranded stability (and associated melting point) as a replacement for deoxythymidine. Phosphorothioate oligos are particularly useful in antisense experiments to inhibit nuclease activity.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/Oligo-flex-2-New.ashx?la=en&hash=7919A1064A605859F90024C3F9C6545D8977EE86" /> </div> <div class="container margin-bottom-30 margin-top-50" style="text-align:left;"> <div class="Libraries-new-content"> <div class="Libraries-content-box"> <div class="row"> <div class="column1" style="background-color:#FFFFFF;"> <h3 style="text-align: center;">5' and 3' Phosphorylation</h3> <p style="text-align: left;">5' phosphorylation can be used for linkers, cloning and gene construction, and ligase- catalyzed ligation reactions. Phosphorylation is also used to prevent DNA chain extension catalyzed by DNA polymerase in related experiments that are resistant to exonuclease digestion.</p> </div> <div class="Libraries-content-box" id="Libraries-content-box-right"> <img alt="" src="/-/media/Images/Oligo-flex-5.ashx?la=en&hash=60FEACE7B1BD5F7C5A3CB095F8A21CC7B372DB5F" /> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Features and Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>High Quality: </strong>Synthetic error rates <0.1% and strict ISO 9001:2015 quality control. All modifications verified by advanced mass spectrometry. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Convenient Deliverables:</strong> Lyophilized oligos are delivered in tube or plate formats after desalting or purification by PAGE or HPLC. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong><strong><span>Flexible Syntheses:</span></strong><span> </span></strong><span>RNA oligos, oligo pools, ultra-pure options, and custom designed oligos are<span style="letter-spacing: 0.05pt;"> </span>available with custom yield options from ng to mg<span style="letter-spacing: -1.85pt;"> </span>quantities<span style="color: red;">.</span></span> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Easy Ordering:</strong> Call, email, or use Azenta’s intuitive online ordering system 24 hours/7 days a week. Our custom ordering form provides additional flexibility for any oligo need. </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>OLIGO Purification Options</h2> <p><span style="color: #000000; background-color: #ffffff; text-align: center;">DNA oligonucleotides are provided in either a tube or plate format, fully lyophilized after purification. Below are recommended purification techniques based on DNA oligo length and required purity.</span></p> <div class="row"> <div class="col-md-9 col-centered"> <table class="table table-responsive table-bordered"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td>Purification</td> <td>Applicable Length</td> <td>Advantages</td> </tr> <tr> <td>Desalted Purification</td> <td>5-90 nt</td> <td style="text-align: center; vertical-align: middle;" class="text-left" rowspan="2">Fast turnaround time and favorable price</td> </tr> <tr> <td rowspan="2">PAGE Purification</td> <td rowspan="2">21-150 nt</td> </tr> <tr> <td style="text-align: center; vertical-align: middle;" class="text-left">High purity >90%; suitable for long oligos</td> </tr> <tr> <td>HPLC Purification</td> <td>5-90 nt</td> <td style="text-align: center; vertical-align: middle;" class="text-left">High purity >95%; suitable for modified oligos</td> </tr> </tbody> </table> </div> </div> </div> <br> <div 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