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})();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_57555652" data-work_id="57555652" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/57555652/Lactobacillus_diolivorans_sp_nov_a_1_2_propanediol_degrading_bacterium_isolated_from_aerobically_stable_maize_silage">Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_57555652" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Inoculation of maize silage with Lactobacillus buchneri (5 x 10(5) c.f.u. g(-1) of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivo...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/57555652" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ab526b3159d3407ee1b00af7613b7f34" rel="nofollow" data-download="{"attachment_id":72401164,"asset_id":57555652,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72401164/download_file?st=MTczMjQ3Mjk2Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32740961" href="https://ugent.academia.edu/JSwings">J. 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After 9 months, lactic acid bacterium counts are approximately 10(10) c.f.u. g(-1) in these treated silages. An important subpopulation (5.9 x 10(7) c.f.u. g(-1)) is able to degrade 1,2-propanediol, a fermentation product of L. buchneri, under anoxic conditions to 1-propanol and propionic acid. From this group of 1,2-propanediol-fermenting, facultatively anaerobic, heterofermentative lactobacilli, two rod-shaped isolates were purified and characterized. Comparative 16S rDNA sequence analysis revealed that the newly isolated bacteria have identical 16S rDNA sequences and belong phylogenetically to the L. buchneri group. DNA-DNA hybridizations, whole-cell protein fingerprinting and examination of phenotypic properties indicated that these two isolates represent a novel species, for which the name Lactobacillus diolivo...","downloadable_attachments":[{"id":72401164,"asset_id":57555652,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32740961,"first_name":"J.","last_name":"Swings","domain_name":"ugent","page_name":"JSwings","display_name":"J. 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Analysis with the nifHD region of Rhizobium... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_68258070" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A modified gel electrophoresis technique provided a reproducible way of detecting and isolating plasmids with molecular weights ranging from 12 X 10(6) to 370 X 10(6) for Azospirillum species. Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all eight strains investigated and not on endogenous plasmids.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/68258070" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ac6ca960f5c6f2f117980ea9f65d6e15" rel="nofollow" data-download="{"attachment_id":78798471,"asset_id":68258070,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/78798471/download_file?st=MTczMjQ3Mjk2Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37761444" href="https://independent.academia.edu/DartPeter">Peter Dart</a><script data-card-contents-for-user="37761444" type="text/json">{"id":37761444,"first_name":"Peter","last_name":"Dart","domain_name":"independent","page_name":"DartPeter","display_name":"Peter Dart","profile_url":"https://independent.academia.edu/DartPeter?f_ri=103360","photo":"https://0.academia-photos.com/37761444/62837812/51135050/s65_peter.dart.png"}</script></span></span></li><li class="js-paper-rank-work_68258070 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="68258070"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 68258070, container: ".js-paper-rank-work_68258070", }); 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Analysis with the nifHD region of Rhizobium trifolii showed that the Azospirillum nif genes were chromosomally located in all eight strains investigated and not on endogenous plasmids.","downloadable_attachments":[{"id":78798471,"asset_id":68258070,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37761444,"first_name":"Peter","last_name":"Dart","domain_name":"independent","page_name":"DartPeter","display_name":"Peter 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u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32566603" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3943b83c22bf2bbaab6aefddf7e3ab03" rel="nofollow" data-download="{"attachment_id":52745459,"asset_id":32566603,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/52745459/download_file?st=MTczMjQ3Mjk2Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down 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data-has-card-for-ri="155" href="https://www.academia.edu/Documents/in/Evolutionary_Biology">Evolutionary Biology</a>, <script data-card-contents-for-ri="155" type="text/json">{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1605" href="https://www.academia.edu/Documents/in/Soil">Soil</a>, <script data-card-contents-for-ri="1605" type="text/json">{"id":1605,"name":"Soil","url":"https://www.academia.edu/Documents/in/Soil?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" 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compost","created_at":"2017-04-21T09:30:06.807-07:00","url":"https://www.academia.edu/32566603/Candidimonas_nitroreducens_gen_nov_sp_nov_and_Candidimonas_humi_sp_nov_isolated_from_sewage_sludge_compost?f_ri=103360","dom_id":"work_32566603","summary":null,"downloadable_attachments":[{"id":52745459,"asset_id":32566603,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":63296130,"first_name":"Ivone","last_name":"Vaz-Moreira","domain_name":"independent","page_name":"IvoneVazMoreira","display_name":"Ivone Vaz-Moreira","profile_url":"https://independent.academia.edu/IvoneVazMoreira?f_ri=103360","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=103360","nofollow":false},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=103360","nofollow":false},{"id":1605,"name":"Soil","url":"https://www.academia.edu/Documents/in/Soil?f_ri=103360","nofollow":false},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=103360","nofollow":false},{"id":8501,"name":"Phospholipids","url":"https://www.academia.edu/Documents/in/Phospholipids?f_ri=103360"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny?f_ri=103360"},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids?f_ri=103360"},{"id":103360,"name":"Nucleic acid hybridization","url":"https://www.academia.edu/Documents/in/Nucleic_acid_hybridization?f_ri=103360"},{"id":112338,"name":"Oxidoreductases","url":"https://www.academia.edu/Documents/in/Oxidoreductases?f_ri=103360"},{"id":131237,"name":"Cluster Analysis","url":"https://www.academia.edu/Documents/in/Cluster_Analysis?f_ri=103360"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=103360"},{"id":139007,"name":"Catalase","url":"https://www.academia.edu/Documents/in/Catalase?f_ri=103360"},{"id":142116,"name":"Quinones","url":"https://www.academia.edu/Documents/in/Quinones?f_ri=103360"},{"id":410387,"name":"Sewage","url":"https://www.academia.edu/Documents/in/Sewage?f_ri=103360"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=103360"},{"id":1223913,"name":"Sodium Chloride","url":"https://www.academia.edu/Documents/in/Sodium_Chloride?f_ri=103360"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=103360"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6311523" data-work_id="6311523" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6311523/Sequence_specific_priming_and_exonuclease_released_fluorescence_assay_for_rapid_and_reliable_HLA_A_molecular_typing">Sequence-specific priming and exonuclease-released fluorescence assay for rapid and reliable HLA-A molecular typing</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div 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class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="9805560" href="https://umich.academia.edu/RajasreeMenon">Rajasree Menon</a><script data-card-contents-for-user="9805560" type="text/json">{"id":9805560,"first_name":"Rajasree","last_name":"Menon","domain_name":"umich","page_name":"RajasreeMenon","display_name":"Rajasree Menon","profile_url":"https://umich.academia.edu/RajasreeMenon?f_ri=103360","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_6311523 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6311523"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new 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type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="18529" href="https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents">Fluorescent Dyes and Reagents</a>, <script data-card-contents-for-ri="18529" type="text/json">{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57808" href="https://www.academia.edu/Documents/in/Cell_line">Cell line</a>, <script data-card-contents-for-ri="57808" type="text/json">{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="61157" href="https://www.academia.edu/Documents/in/Molecular_typing">Molecular typing</a><script data-card-contents-for-ri="61157" type="text/json">{"id":61157,"name":"Molecular typing","url":"https://www.academia.edu/Documents/in/Molecular_typing?f_ri=103360","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6311523]'), work: {"id":6311523,"title":"Sequence-specific priming and exonuclease-released fluorescence assay for rapid and reliable HLA-A molecular typing","created_at":"2014-03-06T04:30:13.677-08:00","url":"https://www.academia.edu/6311523/Sequence_specific_priming_and_exonuclease_released_fluorescence_assay_for_rapid_and_reliable_HLA_A_molecular_typing?f_ri=103360","dom_id":"work_6311523","summary":null,"downloadable_attachments":[{"id":48921361,"asset_id":6311523,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":9805560,"first_name":"Rajasree","last_name":"Menon","domain_name":"umich","page_name":"RajasreeMenon","display_name":"Rajasree Menon","profile_url":"https://umich.academia.edu/RajasreeMenon?f_ri=103360","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=103360","nofollow":false},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=103360","nofollow":false},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=103360","nofollow":false},{"id":61157,"name":"Molecular typing","url":"https://www.academia.edu/Documents/in/Molecular_typing?f_ri=103360","nofollow":false},{"id":103360,"name":"Nucleic acid hybridization","url":"https://www.academia.edu/Documents/in/Nucleic_acid_hybridization?f_ri=103360"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=103360"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=103360"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=103360"},{"id":1006072,"name":"Molecular Diagnosis","url":"https://www.academia.edu/Documents/in/Molecular_Diagnosis?f_ri=103360"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=103360"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6870651" data-work_id="6870651" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6870651/Development_of_molecular_techniques_for_detection_of_lymphocystis_disease_virus_in_different_marine_fish_species">Development of molecular techniques for detection of lymphocystis disease virus in different marine fish species</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Aims: The development and evaluation of a protocol based on polymerase chain reaction (PCR) and nucleic acid hybridization techniques for the specific detection of lymphocystis disease virus (LCDV) in several marine fish species.Methods... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6870651" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Aims: The development and evaluation of a protocol based on polymerase chain reaction (PCR) and nucleic acid hybridization techniques for the specific detection of lymphocystis disease virus (LCDV) in several marine fish species.Methods and Results: The pair of primers for PCR, OBL3 and OBL4, was designed based on published nucleotide sequence (LCDV-1) and amplifies a fragment within the major capsid protein. The sensitivity was evaluated using DNA from purified viral particles, as well as from cells inoculated with several viral concentrations. The PCR combined with slot blot was the most sensitive methodology, detecting 2·5 ng of viral DNA. Using this methodology LCDV was detected at 5 days postinoculation from SAF-1 cells initially inoculated with 10−5 TCID50 ml−1. The combination of PCR with membrane hybridization has also been proved to be adequate to detect LCDV from apparently healthy carriers by means of caudal fin sample analysis. This asymptomatic infection was also demonstrated by classical virological methods (cell culture and immunoblot).Conclusions: The protocol described in this study allows the specific detection of LCDV, both in cell cultures and in fin homogenates from asymptomatic fish.Significance and Impact of the Study: The detection of asymptomatic carriers by a rapid molecular method using caudal fin sampling, which does not imply animal killing, could be an important tool to control epizootics caused by LCDV, as fish could be analysed before their introduction and/or mobilization in farm facilities.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6870651" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="17b595e97252d999a63bd4581c2ec98b" rel="nofollow" data-download="{"attachment_id":48690468,"asset_id":6870651,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48690468/download_file?st=MTczMjQ3Mjk2Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="11499023" href="https://ipv.academia.edu/Patr%C3%ADciaFerro">Patrícia Ferro</a><script data-card-contents-for-user="11499023" type="text/json">{"id":11499023,"first_name":"Patrícia","last_name":"Ferro","domain_name":"ipv","page_name":"PatríciaFerro","display_name":"Patrícia Ferro","profile_url":"https://ipv.academia.edu/Patr%C3%ADciaFerro?f_ri=103360","photo":"https://0.academia-photos.com/11499023/3516089/4128764/s65_patr_cia.ferro.jpg"}</script></span></span></li><li class="js-paper-rank-work_6870651 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6870651"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6870651, container: ".js-paper-rank-work_6870651", }); 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$(".js-view-count[data-work-id=6870651]").text(description); $(".js-view-count-work_6870651").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6870651").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6870651"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="13919" href="https://www.academia.edu/Documents/in/Fish_Diseases">Fish Diseases</a>, <script data-card-contents-for-ri="13919" type="text/json">{"id":13919,"name":"Fish Diseases","url":"https://www.academia.edu/Documents/in/Fish_Diseases?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="19703" href="https://www.academia.edu/Documents/in/Applied_microbiology">Applied microbiology</a>, <script data-card-contents-for-ri="19703" type="text/json">{"id":19703,"name":"Applied microbiology","url":"https://www.academia.edu/Documents/in/Applied_microbiology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57808" href="https://www.academia.edu/Documents/in/Cell_line">Cell line</a><script data-card-contents-for-ri="57808" type="text/json">{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=103360","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6870651]'), work: {"id":6870651,"title":"Development of molecular techniques for detection of lymphocystis disease virus in different marine fish species","created_at":"2014-04-25T08:54:23.601-07:00","url":"https://www.academia.edu/6870651/Development_of_molecular_techniques_for_detection_of_lymphocystis_disease_virus_in_different_marine_fish_species?f_ri=103360","dom_id":"work_6870651","summary":"Aims: The development and evaluation of a protocol based on polymerase chain reaction (PCR) and nucleic acid hybridization techniques for the specific detection of lymphocystis disease virus (LCDV) in several marine fish species.Methods and Results: The pair of primers for PCR, OBL3 and OBL4, was designed based on published nucleotide sequence (LCDV-1) and amplifies a fragment within the major capsid protein. The sensitivity was evaluated using DNA from purified viral particles, as well as from cells inoculated with several viral concentrations. The PCR combined with slot blot was the most sensitive methodology, detecting 2·5 ng of viral DNA. Using this methodology LCDV was detected at 5 days postinoculation from SAF-1 cells initially inoculated with 10−5 TCID50 ml−1. The combination of PCR with membrane hybridization has also been proved to be adequate to detect LCDV from apparently healthy carriers by means of caudal fin sample analysis. This asymptomatic infection was also demonstrated by classical virological methods (cell culture and immunoblot).Conclusions: The protocol described in this study allows the specific detection of LCDV, both in cell cultures and in fin homogenates from asymptomatic fish.Significance and Impact of the Study: The detection of asymptomatic carriers by a rapid molecular method using caudal fin sampling, which does not imply animal killing, could be an important tool to control epizootics caused by LCDV, as fish could be analysed before their introduction and/or mobilization in farm facilities.","downloadable_attachments":[{"id":48690468,"asset_id":6870651,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":11499023,"first_name":"Patrícia","last_name":"Ferro","domain_name":"ipv","page_name":"PatríciaFerro","display_name":"Patrícia Ferro","profile_url":"https://ipv.academia.edu/Patr%C3%ADciaFerro?f_ri=103360","photo":"https://0.academia-photos.com/11499023/3516089/4128764/s65_patr_cia.ferro.jpg"}],"research_interests":[{"id":13919,"name":"Fish Diseases","url":"https://www.academia.edu/Documents/in/Fish_Diseases?f_ri=103360","nofollow":false},{"id":19703,"name":"Applied microbiology","url":"https://www.academia.edu/Documents/in/Applied_microbiology?f_ri=103360","nofollow":false},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=103360","nofollow":false},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=103360","nofollow":false},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment?f_ri=103360"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=103360"},{"id":103360,"name":"Nucleic acid hybridization","url":"https://www.academia.edu/Documents/in/Nucleic_acid_hybridization?f_ri=103360"},{"id":117270,"name":"Fishes","url":"https://www.academia.edu/Documents/in/Fishes?f_ri=103360"},{"id":118339,"name":"Polymerase Chain Reaction","url":"https://www.academia.edu/Documents/in/Polymerase_Chain_Reaction?f_ri=103360"},{"id":238911,"name":"Marine Fish","url":"https://www.academia.edu/Documents/in/Marine_Fish?f_ri=103360"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=103360"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=103360"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10121643" data-work_id="10121643" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10121643/BAC_array_CGH_distinguishes_mutually_exclusive_alterations_that_define_clinicogenetic_subtypes_of_gliomas">BAC array CGH distinguishes mutually exclusive alterations that define clinicogenetic subtypes of gliomas</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The pathological classification of gliomas constitutes a critical step of the clinical management of patients, yet it is frequently challenging. To assess the relationship between genetic abnormalities and clinicopathological... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_10121643" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The pathological classification of gliomas constitutes a critical step of the clinical management of patients, yet it is frequently challenging. To assess the relationship between genetic abnormalities and clinicopathological characteristics, we have performed a genetic and clinical analysis of a series of gliomas. A total of 112 gliomas were analyzed by comparative genomic hybridization on a BAC array with a 1 megabase resolution. Altered regions were identified and correlation analysis enabled to retrieve significant associations and exclusions. Whole chromosomes (chrs) 1p and 19q losses with centromeric breakpoints and EGFR high level amplification were found to be mutually exclusive, permitting identification of 3 distinct, nonoverlapping groups of tumors with striking clinicopathological differences. Type A tumors with chrs 1p and 19q codeletion exhibited an oligodendroglial phenotype and a longer patient survival. Type B tumors were characterized by EGFR amplification. They harbored a WHO high grade of malignancy and a short patient survival. Finally, type C tumors displayed none of the previous patterns but the presence of chr 7 gain, chr 9p deletion and/or chr 10 loss. It included astrocytic tumors in patients younger than in type B and whose prognosis was highly dependent upon the number of alterations. A multivariate analysis based on a Cox model shows that age, WHO grade and genomic type provide complementary prognostic informations. Finally, our results highlight the potential of a whole-genome analysis as an additional diagnostic in cases of unclear conventional genetic findings. © 2007 Wiley-Liss, Inc.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10121643" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="78253fd0c97d20745f4e2e4b760ef61d" rel="nofollow" data-download="{"attachment_id":47519801,"asset_id":10121643,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47519801/download_file?st=MTczMjQ3Mjk2Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="24690491" href="https://independent.academia.edu/IsabelBrito4">Isabel Brito</a><script data-card-contents-for-user="24690491" type="text/json">{"id":24690491,"first_name":"Isabel","last_name":"Brito","domain_name":"independent","page_name":"IsabelBrito4","display_name":"Isabel Brito","profile_url":"https://independent.academia.edu/IsabelBrito4?f_ri=103360","photo":"https://0.academia-photos.com/24690491/47254176/36257463/s65_isabel.brito.jpg"}</script></span></span></li><li class="js-paper-rank-work_10121643 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="10121643"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 10121643, container: ".js-paper-rank-work_10121643", }); 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To assess the relationship between genetic abnormalities and clinicopathological characteristics, we have performed a genetic and clinical analysis of a series of gliomas. A total of 112 gliomas were analyzed by comparative genomic hybridization on a BAC array with a 1 megabase resolution. Altered regions were identified and correlation analysis enabled to retrieve significant associations and exclusions. Whole chromosomes (chrs) 1p and 19q losses with centromeric breakpoints and EGFR high level amplification were found to be mutually exclusive, permitting identification of 3 distinct, nonoverlapping groups of tumors with striking clinicopathological differences. Type A tumors with chrs 1p and 19q codeletion exhibited an oligodendroglial phenotype and a longer patient survival. Type B tumors were characterized by EGFR amplification. They harbored a WHO high grade of malignancy and a short patient survival. Finally, type C tumors displayed none of the previous patterns but the presence of chr 7 gain, chr 9p deletion and/or chr 10 loss. It included astrocytic tumors in patients younger than in type B and whose prognosis was highly dependent upon the number of alterations. A multivariate analysis based on a Cox model shows that age, WHO grade and genomic type provide complementary prognostic informations. Finally, our results highlight the potential of a whole-genome analysis as an additional diagnostic in cases of unclear conventional genetic findings. © 2007 Wiley-Liss, Inc.","downloadable_attachments":[{"id":47519801,"asset_id":10121643,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":24690491,"first_name":"Isabel","last_name":"Brito","domain_name":"independent","page_name":"IsabelBrito4","display_name":"Isabel Brito","profile_url":"https://independent.academia.edu/IsabelBrito4?f_ri=103360","photo":"https://0.academia-photos.com/24690491/47254176/36257463/s65_isabel.brito.jpg"}],"research_interests":[{"id":6021,"name":"Cancer","url":"https://www.academia.edu/Documents/in/Cancer?f_ri=103360","nofollow":false},{"id":10610,"name":"Survival Analysis","url":"https://www.academia.edu/Documents/in/Survival_Analysis?f_ri=103360","nofollow":false},{"id":41482,"name":"Multivariate Analysis","url":"https://www.academia.edu/Documents/in/Multivariate_Analysis?f_ri=103360","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=103360","nofollow":false},{"id":76383,"name":"Glioma","url":"https://www.academia.edu/Documents/in/Glioma?f_ri=103360"},{"id":98925,"name":"Female","url":"https://www.academia.edu/Documents/in/Female?f_ri=103360"},{"id":103360,"name":"Nucleic acid hybridization","url":"https://www.academia.edu/Documents/in/Nucleic_acid_hybridization?f_ri=103360"},{"id":111545,"name":"Male","url":"https://www.academia.edu/Documents/in/Male?f_ri=103360"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=103360"},{"id":295155,"name":"Middle Aged","url":"https://www.academia.edu/Documents/in/Middle_Aged?f_ri=103360"},{"id":382075,"name":"Adult","url":"https://www.academia.edu/Documents/in/Adult?f_ri=103360"},{"id":489727,"name":"Prognosis","url":"https://www.academia.edu/Documents/in/Prognosis?f_ri=103360"},{"id":521881,"name":"World Health Organization","url":"https://www.academia.edu/Documents/in/World_Health_Organization?f_ri=103360"},{"id":859051,"name":"Astrocytoma","url":"https://www.academia.edu/Documents/in/Astrocytoma?f_ri=103360"},{"id":1272981,"name":"Proportional Hazards Models","url":"https://www.academia.edu/Documents/in/Proportional_Hazards_Models?f_ri=103360"},{"id":1318932,"name":"Predictive value of tests","url":"https://www.academia.edu/Documents/in/Predictive_value_of_tests?f_ri=103360"},{"id":1425045,"name":"Brain 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href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="37833" href="https://www.academia.edu/Documents/in/Real-time_RT-PCR">Real-time RT-PCR</a>, <script data-card-contents-for-ri="37833" type="text/json">{"id":37833,"name":"Real-time RT-PCR","url":"https://www.academia.edu/Documents/in/Real-time_RT-PCR?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="51784" href="https://www.academia.edu/Documents/in/real_time_PCR">real time PCR</a><script data-card-contents-for-ri="51784" type="text/json">{"id":51784,"name":"real time PCR","url":"https://www.academia.edu/Documents/in/real_time_PCR?f_ri=103360","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22615508]'), work: {"id":22615508,"title":"Rapid detection and simultaneous subtype differentiation of influenza A viruses by real time 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Time","url":"https://www.academia.edu/Documents/in/Real_Time?f_ri=103360"},{"id":561000,"name":"influenza A virus","url":"https://www.academia.edu/Documents/in/influenza_A_virus?f_ri=103360"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=103360"},{"id":901876,"name":"Sensitivity and Specificity","url":"https://www.academia.edu/Documents/in/Sensitivity_and_Specificity?f_ri=103360"},{"id":2104033,"name":"Melting Temperature","url":"https://www.academia.edu/Documents/in/Melting_Temperature?f_ri=103360"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_20649800 coauthored" data-work_id="20649800" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/20649800/The_Drosophila_EGF_receptor_gene_homolog_Conservation_of_both_hormone_binding_and_kinase_domains">The Drosophila EGF receptor gene homolog: Conservation of both hormone binding and kinase domains</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Chicken v-erB probe was used to isolate a unique clone of Drosophila melanogaster DNA. It maps by in situ hybridization to position 57F on chromosome 2. A complete nucleotide sequence of the coding region has been obtained. The putative... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_20649800" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Chicken v-erB probe was used to isolate a unique clone of Drosophila melanogaster DNA. It maps by in situ hybridization to position 57F on chromosome 2. A complete nucleotide sequence of the coding region has been obtained. The putative Drosophila EGF receptor protein is similar in overall organization to the human homolog. It shows three distinct domains: an extracellular putative EGF binding domain, a hydrophobic transmembrane region, and a cytoplasmic kinase domain. The overall amino acid homology is 41% in the extracellular domain and 55% in the kinase domain. Two cysteine-rich regions, a hallmark of the human ligand-binding domain, have also been conserved. Fusion of the coding sequences of the kinase and extracellular domains generating the receptor gene must have occurred over 800 million years ago.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/20649800" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="40898371" href="https://independent.academia.edu/EttaLivneh">Etta Livneh</a><script data-card-contents-for-user="40898371" type="text/json">{"id":40898371,"first_name":"Etta","last_name":"Livneh","domain_name":"independent","page_name":"EttaLivneh","display_name":"Etta Livneh","profile_url":"https://independent.academia.edu/EttaLivneh?f_ri=103360","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-20649800">+1</span><div class="hidden js-additional-users-20649800"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/SchlessingerJ">J. Schlessinger</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-20649800'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-20649800').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_20649800 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="20649800"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 20649800, container: ".js-paper-rank-work_20649800", }); });</script></li><li class="js-percentile-work_20649800 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 20649800; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_20649800"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_20649800 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="20649800"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 20649800; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=20649800]").text(description); $(".js-view-count-work_20649800").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_20649800").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="20649800"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="8090" href="https://www.academia.edu/Documents/in/Bacteriophages">Bacteriophages</a>, <script data-card-contents-for-ri="8090" type="text/json">{"id":8090,"name":"Bacteriophages","url":"https://www.academia.edu/Documents/in/Bacteriophages?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12768" href="https://www.academia.edu/Documents/in/Drosophila_melanogaster">Drosophila melanogaster</a>, <script data-card-contents-for-ri="12768" type="text/json">{"id":12768,"name":"Drosophila melanogaster","url":"https://www.academia.edu/Documents/in/Drosophila_melanogaster?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="41012" href="https://www.academia.edu/Documents/in/Recombinant_DNA_Technology">Recombinant DNA Technology</a>, <script data-card-contents-for-ri="41012" type="text/json">{"id":41012,"name":"Recombinant DNA Technology","url":"https://www.academia.edu/Documents/in/Recombinant_DNA_Technology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=103360","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=20649800]'), work: {"id":20649800,"title":"The Drosophila EGF receptor gene homolog: Conservation of both hormone binding and kinase domains","created_at":"2016-01-23T10:55:24.980-08:00","url":"https://www.academia.edu/20649800/The_Drosophila_EGF_receptor_gene_homolog_Conservation_of_both_hormone_binding_and_kinase_domains?f_ri=103360","dom_id":"work_20649800","summary":"Chicken v-erB probe was used to isolate a unique clone of Drosophila melanogaster DNA. It maps by in situ hybridization to position 57F on chromosome 2. A complete nucleotide sequence of the coding region has been obtained. The putative Drosophila EGF receptor protein is similar in overall organization to the human homolog. It shows three distinct domains: an extracellular putative EGF binding domain, a hydrophobic transmembrane region, and a cytoplasmic kinase domain. The overall amino acid homology is 41% in the extracellular domain and 55% in the kinase domain. Two cysteine-rich regions, a hallmark of the human ligand-binding domain, have also been conserved. Fusion of the coding sequences of the kinase and extracellular domains generating the receptor gene must have occurred over 800 million years ago.","downloadable_attachments":[],"ordered_authors":[{"id":40898371,"first_name":"Etta","last_name":"Livneh","domain_name":"independent","page_name":"EttaLivneh","display_name":"Etta Livneh","profile_url":"https://independent.academia.edu/EttaLivneh?f_ri=103360","photo":"/images/s65_no_pic.png"},{"id":211868897,"first_name":"J.","last_name":"Schlessinger","domain_name":"independent","page_name":"SchlessingerJ","display_name":"J. Schlessinger","profile_url":"https://independent.academia.edu/SchlessingerJ?f_ri=103360","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":8090,"name":"Bacteriophages","url":"https://www.academia.edu/Documents/in/Bacteriophages?f_ri=103360","nofollow":false},{"id":12768,"name":"Drosophila melanogaster","url":"https://www.academia.edu/Documents/in/Drosophila_melanogaster?f_ri=103360","nofollow":false},{"id":41012,"name":"Recombinant DNA Technology","url":"https://www.academia.edu/Documents/in/Recombinant_DNA_Technology?f_ri=103360","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=103360","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=103360"},{"id":69424,"name":"Protein Kinases","url":"https://www.academia.edu/Documents/in/Protein_Kinases?f_ri=103360"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=103360"},{"id":103360,"name":"Nucleic acid hybridization","url":"https://www.academia.edu/Documents/in/Nucleic_acid_hybridization?f_ri=103360"},{"id":107533,"name":"Cell","url":"https://www.academia.edu/Documents/in/Cell?f_ri=103360"},{"id":172083,"name":"Phosphorylation","url":"https://www.academia.edu/Documents/in/Phosphorylation?f_ri=103360"},{"id":191815,"name":"Biological evolution","url":"https://www.academia.edu/Documents/in/Biological_evolution?f_ri=103360"},{"id":329263,"name":"Epidermal Growth Factor","url":"https://www.academia.edu/Documents/in/Epidermal_Growth_Factor?f_ri=103360"},{"id":402759,"name":"Chickens","url":"https://www.academia.edu/Documents/in/Chickens?f_ri=103360"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=103360"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence?f_ri=103360"},{"id":1128165,"name":"EGF receptor","url":"https://www.academia.edu/Documents/in/EGF_receptor?f_ri=103360"},{"id":1166930,"name":"Cytoplasm","url":"https://www.academia.edu/Documents/in/Cytoplasm?f_ri=103360"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_18401424" data-work_id="18401424" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/18401424/Gluconobacter_nephelii_sp_nov_an_acetic_acid_bacterium_in_the_alpha_Proteobacteria">Gluconobacter nephelii sp. nov., an acetic acid bacterium in the {alpha}-Proteobacteria</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Three strains, RBY-1T, PHD-1, and PHD-2 were isolated from fruits in Thailand. The strains were Gram-negative, aerobic rods with polar flagella, produced acetic acid from ethanol and did not oxidize acetate and lactate. In phylogenetic... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_18401424" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Three strains, RBY-1T, PHD-1, and PHD-2 were isolated from fruits in Thailand. The strains were Gram-negative, aerobic rods with polar flagella, produced acetic acid from ethanol and did not oxidize acetate and lactate. In phylogenetic trees based on 16S rRNA gene sequences and 16S-23S rRNA gene ITS sequences, the strains formed a cluster separate from the type strains of the eleven species of the genus Gluconobacter. The calculated 16S rRNA gene sequence and 16S-23S rRNA gene ITS sequence similarities were respectively 97.7-99.7% and 77.3-98.1%. DNA G+C contents were 57.2-57.6 mol%. They showed high DNA-DNA relatedness of 100% with one another but low DNA-DNA relatedness of 11-34% with the type strains of the eleven Gluconobacter species. Q-10 was a major quinone. On the basis of the genotypic and phenotypic data obtained, the three strains clearly represent a novel species, for which the name Gluconobacter nephelii sp. nov. is proposed. The type strain is strain RBY-1T (= BCC 3673...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18401424" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8454ef6930113d67d4e1977f18c29121" rel="nofollow" data-download="{"attachment_id":40035040,"asset_id":18401424,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40035040/download_file?st=MTczMjQ3Mjk2NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38402070" href="https://independent.academia.edu/SomboonTanasupawat">Somboon Tanasupawat</a><script data-card-contents-for-user="38402070" type="text/json">{"id":38402070,"first_name":"Somboon","last_name":"Tanasupawat","domain_name":"independent","page_name":"SomboonTanasupawat","display_name":"Somboon Tanasupawat","profile_url":"https://independent.academia.edu/SomboonTanasupawat?f_ri=103360","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_18401424 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="18401424"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 18401424, container: ".js-paper-rank-work_18401424", }); 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The strains were Gram-negative, aerobic rods with polar flagella, produced acetic acid from ethanol and did not oxidize acetate and lactate. In phylogenetic trees based on 16S rRNA gene sequences and 16S-23S rRNA gene ITS sequences, the strains formed a cluster separate from the type strains of the eleven species of the genus Gluconobacter. The calculated 16S rRNA gene sequence and 16S-23S rRNA gene ITS sequence similarities were respectively 97.7-99.7% and 77.3-98.1%. DNA G+C contents were 57.2-57.6 mol%. They showed high DNA-DNA relatedness of 100% with one another but low DNA-DNA relatedness of 11-34% with the type strains of the eleven Gluconobacter species. Q-10 was a major quinone. On the basis of the genotypic and phenotypic data obtained, the three strains clearly represent a novel species, for which the name Gluconobacter nephelii sp. nov. is proposed. The type strain is strain RBY-1T (= BCC 3673...","downloadable_attachments":[{"id":40035040,"asset_id":18401424,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38402070,"first_name":"Somboon","last_name":"Tanasupawat","domain_name":"independent","page_name":"SomboonTanasupawat","display_name":"Somboon Tanasupawat","profile_url":"https://independent.academia.edu/SomboonTanasupawat?f_ri=103360","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=103360","nofollow":false},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=103360","nofollow":false},{"id":6947,"name":"Medical 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assay","created_at":"2021-11-06T18:58:34.565-07:00","url":"https://www.academia.edu/61146498/Development_of_a_method_for_the_detection_of_infectious_myonecrosis_virus_by_reverse_transcription_loop_mediated_isothermal_amplification_and_nucleic_acid_lateral_flow_hybrid_assay?f_ri=103360","dom_id":"work_61146498","summary":null,"downloadable_attachments":[{"id":74286693,"asset_id":61146498,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":184661747,"first_name":"Thales","last_name":"Andrade","domain_name":"independent","page_name":"ThalesAndrade17","display_name":"Thales 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alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment?f_ri=103360"},{"id":99234,"name":"Animals","url":"https://www.academia.edu/Documents/in/Animals?f_ri=103360"},{"id":103360,"name":"Nucleic acid hybridization","url":"https://www.academia.edu/Documents/in/Nucleic_acid_hybridization?f_ri=103360"},{"id":170652,"name":"Fisheries Sciences","url":"https://www.academia.edu/Documents/in/Fisheries_Sciences?f_ri=103360"},{"id":178906,"name":"Nucleic Acids","url":"https://www.academia.edu/Documents/in/Nucleic_Acids?f_ri=103360"},{"id":207615,"name":"Hybrids","url":"https://www.academia.edu/Documents/in/Hybrids?f_ri=103360"},{"id":237339,"name":"EXTRACTION","url":"https://www.academia.edu/Documents/in/EXTRACTION?f_ri=103360"},{"id":549280,"name":"Reproducibility of Results","url":"https://www.academia.edu/Documents/in/Reproducibility_of_Results?f_ri=103360"},{"id":644860,"name":"Veterinary 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u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Four orange-pigmented isolates, L7-456, L7-484T, L9-479 and L9-753T, originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_55391563" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Four orange-pigmented isolates, L7-456, L7-484T, L9-479 and L9-753T, originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas . In these analyses, strain L7-484T appeared to be most closely related to Aureimonas ureilytica 5715S-12T (95.7 % sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753T were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12T (97.5 %). Both L7-484T and L9-753T contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C18 : 1ω7c and C16 : 0 were the major fa...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/55391563" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b6b754b6a00e519e89b234971f6b6f83" rel="nofollow" data-download="{"attachment_id":71282679,"asset_id":55391563,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/71282679/download_file?st=MTczMjQ3Mjk2NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="79618912" href="https://independent.academia.edu/MunusamyMadhaiyan">Munusamy Madhaiyan</a><script data-card-contents-for-user="79618912" type="text/json">{"id":79618912,"first_name":"Munusamy","last_name":"Madhaiyan","domain_name":"independent","page_name":"MunusamyMadhaiyan","display_name":"Munusamy Madhaiyan","profile_url":"https://independent.academia.edu/MunusamyMadhaiyan?f_ri=103360","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_55391563 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="55391563"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 55391563, container: ".js-paper-rank-work_55391563", }); 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Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas . In these analyses, strain L7-484T appeared to be most closely related to Aureimonas ureilytica 5715S-12T (95.7 % sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753T were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12T (97.5 %). Both L7-484T and L9-753T contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C18 : 1ω7c and C16 : 0 were the major fa...","downloadable_attachments":[{"id":71282679,"asset_id":55391563,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":79618912,"first_name":"Munusamy","last_name":"Madhaiyan","domain_name":"independent","page_name":"MunusamyMadhaiyan","display_name":"Munusamy Madhaiyan","profile_url":"https://independent.academia.edu/MunusamyMadhaiyan?f_ri=103360","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology?f_ri=103360","nofollow":false},{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=103360","nofollow":false},{"id":6947,"name":"Medical 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u-tcGrayDarkest"><div class="summarized">Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA &quot;chips&quot; were used to quantitatively monitor differential expression of the cognate human genes using a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_76159960" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA &quot;chips&quot; were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/76159960" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ca291017241170b8fe3afe036df7da6a" rel="nofollow" data-download="{"attachment_id":83994770,"asset_id":76159960,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/83994770/download_file?st=MTczMjQ3Mjk2NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="99627361" href="https://independent.academia.edu/MarkSchena">Mark Schena</a><script data-card-contents-for-user="99627361" type="text/json">{"id":99627361,"first_name":"Mark","last_name":"Schena","domain_name":"independent","page_name":"MarkSchena","display_name":"Mark Schena","profile_url":"https://independent.academia.edu/MarkSchena?f_ri=103360","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_76159960 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="76159960"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 76159960, container: ".js-paper-rank-work_76159960", }); 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to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus spp. (large strongyles) was demonstrated. The assay relied on the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13574104" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The ability of a reverse line blot (RLB) assay to identify 13 common species of equine small strongyles (cyathostomins) and to discriminate them from three Strongylus spp. (large strongyles) was demonstrated. The assay relied on the specific hybridization of PCR-amplified intergenic spacer DNA fragments of the nuclear ribosomal DNA to membrane-bound species-specific probes. All cyathostomins examined were unequivocally identified and</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13574104" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="61b764109d76b4aa4bb27e102c5b6fa7" rel="nofollow" data-download="{"attachment_id":38078168,"asset_id":13574104,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/38078168/download_file?st=MTczMjQ3Mjk2NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32756279" href="https://independent.academia.edu/RaffaellaIorio">Raffaella Iorio</a><script data-card-contents-for-user="32756279" type="text/json">{"id":32756279,"first_name":"Raffaella","last_name":"Iorio","domain_name":"independent","page_name":"RaffaellaIorio","display_name":"Raffaella Iorio","profile_url":"https://independent.academia.edu/RaffaellaIorio?f_ri=103360","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13574104">+1</span><div class="hidden js-additional-users-13574104"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://izan-kiev.academia.edu/VitaliyKharchenko">Vitaliy Kharchenko</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13574104'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13574104').html(); 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type="text/json">{"id":41012,"name":"Recombinant DNA Technology","url":"https://www.academia.edu/Documents/in/Recombinant_DNA_Technology?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="48057" href="https://www.academia.edu/Documents/in/DNA">DNA</a>, <script data-card-contents-for-ri="48057" type="text/json">{"id":48057,"name":"DNA","url":"https://www.academia.edu/Documents/in/DNA?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="99234" href="https://www.academia.edu/Documents/in/Animals">Animals</a><script data-card-contents-for-ri="99234" 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type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=103360","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="73865" href="https://www.academia.edu/Documents/in/Potentiometry">Potentiometry</a><script data-card-contents-for-ri="73865" type="text/json">{"id":73865,"name":"Potentiometry","url":"https://www.academia.edu/Documents/in/Potentiometry?f_ri=103360","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=26931909]'), work: {"id":26931909,"title":"Amplified detection of DNA hybridization using post-labelling with a biotin-modified 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