Search results for: J. Macione

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Macione</title> <meta name="description" content="Search results for: J. Macione"> <meta name="keywords" content="J. Macione"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="J. 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Macione"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: J. Macione</h1> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Realignment of f-actin Cytoskeleton in Osteocytes after Mechanical Loading</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=R.%20S.%20A.%20Nesbitt">R. S. A. Nesbitt</a>, <a href="https://publications.waset.org/search?q=J.%20Macione"> J. Macione</a>, <a href="https://publications.waset.org/search?q=E.%20Babollah"> E. Babollah</a>, <a href="https://publications.waset.org/search?q=B.%20Adu-baffour"> B. Adu-baffour</a>, <a href="https://publications.waset.org/search?q=S.%20P.%20Kotha"> S. P. Kotha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> F-actin fibrils are the cytoskeleton of osteocytes. They react in a dynamic manner to mechanical loading, and strength and reposition their efforts to reinforce the cells structure. We hypothesize that f-actin is temporarly disrupted after loading and repolymerizes in a new orientation to oppose the applied load. In vitro studies are conducted to determine f-actin disruption after varying mechanical stimulus parameters that are known to affect bone formation. Results indicate that the f-actin cytoskeleton is disrupted in vitro as a function of applied mechanical stimulus parameters and that the f-actin bundles reassemble after loading induced disruption within 3 minutes after cessation of loading. The disruption of the factin cytoskeleton depends on the magnitude of stretch, the numbers of loading cycles, frequency, the insertion of rest between loading cycles and extracellular calcium. In vivo studies also demonstrate disruption of the f-actin cytoskeleton in cells embedded in the bone matrix immediately after mechanical loading. These studies suggest that adaptation of the f-actin fiber bundles of the cytoskeleton in response to applied loads occurs by disruption and subsequent repolymerization. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Mechanical%20loading%20of%20osteocytes" title="Mechanical loading of osteocytes">Mechanical loading of osteocytes</a>, <a href="https://publications.waset.org/search?q=f-actin%20cytoskeleton" title=" f-actin cytoskeleton"> f-actin cytoskeleton</a>, <a href="https://publications.waset.org/search?q=disruption" title=" disruption"> disruption</a>, <a href="https://publications.waset.org/search?q=re-polymerization." title=" re-polymerization."> re-polymerization.</a> </p> <a href="https://publications.waset.org/2128/realignment-of-f-actin-cytoskeleton-in-osteocytes-after-mechanical-loading" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/2128/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/2128/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/2128/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/2128/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/2128/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/2128/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/2128/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/2128/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/2128/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/2128/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/2128.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1561</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Bone Generation through Mechanical Loading</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=R.%20S.%20A.%20Nesbitt">R. S. A. Nesbitt</a>, <a href="https://publications.waset.org/search?q=J.%20Macione"> J. Macione</a>, <a href="https://publications.waset.org/search?q=A.%20Debroy"> A. Debroy</a>, <a href="https://publications.waset.org/search?q=S.%20P.%20Kotha"> S. P. Kotha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bones are dynamic and responsive organs, they regulate their strength and mass according to the loads which they are subjected. Because, the Wnt/尾-catenin pathway has profound effects on the regulation of bone mass, we hypothesized that mechanical loading of bone cells stimulates Wnt/尾-catenin signaling, which results in the generation of new bone mass. Mechanical loading triggers the secretion of the Wnt molecule, which after binding to transmembrane proteins, causes GSK-3尾 (Glycogen synthase kinase 3 beta) to cease the phosphorylation of 尾-catenin. 尾-catenin accumulation in the cytoplasm, followed by its transport into the nucleus, binding to transcription factors (TCF/LEF) that initiate transcription of genes related to bone formation. To test this hypothesis, we used TOPGAL (Tcf Optimal Promoter 尾-galactosidase) mice in an experiment in which cyclic loads were applied to the forearm. TOPGAL mice are reporters for cells effected by the Wnt/尾-catenin signaling pathway. TOPGAL mice are genetically engineered mice in which transcriptional activation of 尾- catenin, results in the production of an enzyme, 尾-galactosidase. The presence of this enzyme allows us to localize transcriptional activation of 尾-catenin to individual cells, thereby, allowing us to quantify the effects that mechanical loading has on the Wnt/尾-catenin pathway and new bone formation. The ulnae of loaded TOPGAL mice were excised and transverse slices along different parts of the ulnar shaft were assayed for the presence of 尾-galactosidase. Our results indicate that loading increases 尾-catenin transcriptional activity in regions where this pathway is already primed (i.e. where basal activity is already higher) in a load magnitude dependent manner. Further experiments are needed to determine the temporal and spatial activation of this signaling in relation to bone formation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Bone%20Resorption%20and%20Formation" title="Bone Resorption and Formation">Bone Resorption and Formation</a>, <a href="https://publications.waset.org/search?q=Mechanical%20Loading%20of%20Bone" title=" Mechanical Loading of Bone"> Mechanical Loading of Bone</a>, <a href="https://publications.waset.org/search?q=Wnt%20Signaling%20Pathway%20%26%20%CE%B2-catenin." title=" Wnt Signaling Pathway & 尾-catenin."> Wnt Signaling Pathway & 尾-catenin.</a> </p> <a href="https://publications.waset.org/761/bone-generation-through-mechanical-loading" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/761/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/761/bibtex" target="_blank" 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Search results for: J. Macione