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experiments</title> <meta name="description" content="Search results for: in vitro experiments"> <meta name="keywords" content="in vitro experiments"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="in vitro experiments"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4545</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: in vitro experiments</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4545</span> Self-Carried Theranostic Nanoparticles for in vitro and in vivo Cancer Therapy with Real-Time Monitoring of Drug Release</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jinfeng%20Zhang">Jinfeng Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Chun-Sing%20Lee"> Chun-Sing Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The use of different nanocarriers for delivering hydrophobic pharmaceutical agents to tumor sites has garnered major attention. Despite the merits of these nanocarriers, further studies are needed for improving their drug loading capacities (typically less than 10%) and reducing their potential systemic toxicity. So development of alternative self-carried nanodrug delivery strategies without using any inert carriers is highly desirable. In this study, we developed a self-carried theranostic curcumin (Cur) nanodrug for highly effective cancer therapy in vitro and in vivo with real-time monitoring of drug release. With a biocompatible C18PMH-PEG functionalization, the Cur nanoparticles (NPs) showed excellent dispersibility and outstanding stability in physiological environment, with drug loading capacity higher than 78 wt.%. Both confocal microscopy and flow cytometry confirmed the cellular fluorescent “OFF-ON” activation and real-time monitoring of Cur molecule release, showing its potential for cancer diagnosis. In vitro and in vivo experiments clearly show that therapeutic efficacy of the PEGylated Cur NPs is much better than that of free Cur. This self-carried theranostic strategy with real-time monitoring of drug release may open a new way for simultaneous cancer therapy and diagnosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20delivery" title="drug delivery">drug delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20and%20in%20vivo%20cancer%20therapy" title=" in vitro and in vivo cancer therapy"> in vitro and in vivo cancer therapy</a>, <a href="https://publications.waset.org/abstracts/search?q=real-time%20monitoring" title=" real-time monitoring"> real-time monitoring</a>, <a href="https://publications.waset.org/abstracts/search?q=self-carried" title=" self-carried"> self-carried</a> </p> <a href="https://publications.waset.org/abstracts/26667/self-carried-theranostic-nanoparticles-for-in-vitro-and-in-vivo-cancer-therapy-with-real-time-monitoring-of-drug-release" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26667.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">399</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4544</span> Factors Affecting the Results of in vitro Gas Production Technique</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=O.%20Kahraman">O. Kahraman</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20S.%20Alatas"> M. S. Alatas</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20B.%20Citil"> O. B. Citil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In determination of values of feeds which, are used in ruminant nutrition, different methods are used like in vivo, in vitro, in situ or in sacco. Generally, the most reliable results are taken from the in vivo studies. But because of the disadvantages like being hard, laborious and expensive, time consuming, being hard to keep the experiment conditions under control and too much samples are needed, the in vitro techniques are more preferred. The most widely used in vitro techniques are two-staged digestion technique and gas production technique. In vitro gas production technique is based on the measurement of the CO2 which is released as a result of microbial fermentation of the feeds. In this review, the factors affecting the results obtained from in vitro gas production technique (Hohenheim Feed Test) were discussed. Some factors must be taken into consideration when interpreting the findings obtained in these studies and also comparing the findings reported by different researchers for the same feeds. These factors were discussed in 3 groups: factors related to animal, factors related to feeds and factors related with differences in the application of method. These factors and their effects on the results were explained. Also it can be concluded that the use of in vitro gas production technique in feed evaluation routinely can be contributed to the comprehensive feed evaluation, but standardization is needed in this technique to attain more reliable results. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=In%20vitro" title="In vitro">In vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20production%20technique" title=" gas production technique"> gas production technique</a>, <a href="https://publications.waset.org/abstracts/search?q=Hohenheim%20feed%20test" title=" Hohenheim feed test"> Hohenheim feed test</a>, <a href="https://publications.waset.org/abstracts/search?q=standardization" title=" standardization"> standardization</a> </p> <a href="https://publications.waset.org/abstracts/26010/factors-affecting-the-results-of-in-vitro-gas-production-technique" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26010.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">599</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4543</span> The Impact of Garlic and Citrus Extracts on Energy Retention and Methane Production in Ruminants in vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Michael%20Graz">Michael Graz</a>, <a href="https://publications.waset.org/abstracts/search?q=Natasha%20Hurril"> Natasha Hurril</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrew%20Shearer"> Andrew Shearer</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Research on feed supplementation with natural compounds is currently being intensively pursued with a view to improving energy utilisation in ruminants and mitigating the production of methane by these animals. Towards this end, a novel combination of extracts from garlic and bitter orange was therefore selected for trials on the basis of their previously published in vitro anti-methanogenic potential. Three separate in vitro experiments were conducted to determine energy utilisation and greenhouse gas production. These included use of rumen fluid from fistulated cows and sheep in batch culture, the Hohenheim gas test, and the Rusitec technique. Experimental and control arms were utilised, with 5g extracts per kilogram of total dietary dry matter (0.05g/kg active compounds) being used to supplement or not supplement the in vitro systems. Respiratory measurements were conducted on experimental day 1 for the batch culture and Hohenheim gas test and on day 14-21 for the Rusitec Technique (in a 21-day trial). Measurements included methane (CH4) production, total volatile fatty acid (VFA) concentration, molar proportions of acetate, propionate and butyrate and degradation of organic matter (Rusitec). CH4 production was reduced by 82% (±16%), 68% (±11%) and 37% (±4%) in the batch culture, Hohenheim gas test and Rusitec, respectively. Total VFA production was reduced by 13% (±2%) and 2% (±0.1%) in the batch culture and Hohenheim gas test whilst it was increased by 8% (±2%) in the Rusitec. Total VFA production was reduced in all tests between 2 and 10%, whilst acetate production was reduced between 10% and 29%. Propionate production which is an indicator of weight gain was increased in all cases between 16% and 30%. Butyrate production which is considered an indicator of potential milk yield was increased by between 6 and 11%. Degradation of organic matter in the Rusitec experiments was improved by 10% (±0.1%). In conclusion, the study demonstrated the potential of the combination of garlic and citrus extracts to improve digestion, enhance body energy retention and limit CH4 formation in relation to feed intake. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=citrus" title="citrus">citrus</a>, <a href="https://publications.waset.org/abstracts/search?q=garlic" title=" garlic"> garlic</a>, <a href="https://publications.waset.org/abstracts/search?q=methane" title=" methane"> methane</a>, <a href="https://publications.waset.org/abstracts/search?q=ruminants" title=" ruminants"> ruminants</a> </p> <a href="https://publications.waset.org/abstracts/67828/the-impact-of-garlic-and-citrus-extracts-on-energy-retention-and-methane-production-in-ruminants-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67828.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">330</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4542</span> Electro-Thermal Imaging of Breast Phantom: An Experimental Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=H.%20Feza%20Carlak">H. Feza Carlak</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20G.%20Gencer"> N. G. Gencer</a> </p> <p class="card-text"><strong>Abstract:</strong></p> To increase the temperature contrast in thermal images, the characteristics of the electrical conductivity and thermal imaging modalities can be combined. In this experimental study, it is objected to observe whether the temperature contrast created by the tumor tissue can be improved just due to the current application within medical safety limits. Various thermal breast phantoms are developed to simulate the female breast tissue. In vitro experiments are implemented using a thermal infrared camera in a controlled manner. Since experiments are implemented in vitro, there is no metabolic heat generation and blood perfusion. Only the effects and results of the electrical stimulation are investigated. Experimental study is implemented with two-dimensional models. Temperature contrasts due to the tumor tissues are obtained. Cancerous tissue is determined using the difference and ratio of healthy and tumor images. 1 cm diameter single tumor tissue causes almost 40 °mC temperature contrast on the thermal-breast phantom. Electrode artifacts are reduced by taking the difference and ratio of background (healthy) and tumor images. Ratio of healthy and tumor images show that temperature contrast is increased by the current application. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=medical%20diagnostic%20imaging" title="medical diagnostic imaging">medical diagnostic imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20phantom" title=" breast phantom"> breast phantom</a>, <a href="https://publications.waset.org/abstracts/search?q=active%20thermography" title=" active thermography"> active thermography</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer%20detection" title=" breast cancer detection"> breast cancer detection</a> </p> <a href="https://publications.waset.org/abstracts/7912/electro-thermal-imaging-of-breast-phantom-an-experimental-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/7912.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">428</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4541</span> Study of Toxic Effect and Anti-Oxidative Activity of a β- Amidophosphonates</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Houria%20Djebar">Houria Djebar</a>, <a href="https://publications.waset.org/abstracts/search?q=Amina%20Saib"> Amina Saib</a>, <a href="https://publications.waset.org/abstracts/search?q=Malika%20Berredjem"> Malika Berredjem</a>, <a href="https://publications.waset.org/abstracts/search?q=Khaoula%20Bechlem"> Khaoula Bechlem</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed-Reda%20Djebar"> Mohammed-Reda Djebar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Reactive oxygen species (ROS) have a high potential to damage almost all types of cellular components of the body, which explains their involvement in the induction and/or amplification of several pathologies. Supplementation of the body by exogenous antioxidants is very useful against these harmful species. In this context, we attempted to evaluate the in vitro and in vivo antioxidant activities of three newly synthesized amidophosphonates (AP1, AP2, and AP3). The results relating to the in vitro tests for DPPH radical scavenging activity shows that these amidophosphonates have a modest antiradical power (ARP) less effectively pronounced compared with an analogue marketed in Algeria: (Dursban) Clorpiryphos ethyl. However, in vivo effects were evaluated on some antioxidant systems (LP intensity, CAT activity and GSH content), or in combination with 2, 2-diphenyl-picrylhydrazyle (DPPH) radical in paramecium tetraurelia used as a complementary system to rapidly elucidate the cytotoxicity. On the basis of the results obtained it can be concluded that amidophosphonates studied exhibited a mild protective effect. The mechanism for how they influenced the antioxidant activities was discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Paramecium%20tetraurelia" title="Paramecium tetraurelia">Paramecium tetraurelia</a>, <a href="https://publications.waset.org/abstracts/search?q=amidophosphonates" title=" amidophosphonates"> amidophosphonates</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH%20free%20radical" title=" DPPH free radical"> DPPH free radical</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments" title=" in vitro experiments"> in vitro experiments</a>, <a href="https://publications.waset.org/abstracts/search?q=biochemical%20parameters" title=" biochemical parameters"> biochemical parameters</a> </p> <a href="https://publications.waset.org/abstracts/106698/study-of-toxic-effect-and-anti-oxidative-activity-of-a-v-amidophosphonates" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/106698.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">165</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4540</span> Feed Value of Selected Nigerian Browse Plants: Chemical Composition and in vitro Digestibility</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Isaac%20Samuel">Isaac Samuel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A study was conducted to determine the in-vitro degradation of selected Nigerian browse plants consumed by small ruminants on free range in northern guinea savannah region of Nigeria using in vitro gas production, proximate composition, fibre components, methane gas production and dry matter degradation as tools. The leaves samples of the selected browse plants were collected, processed and incubated using in vitro gas dry matter degradation techniques. Results obtained showed variation in the rate of degradation. The result obtained from chemical analysis showed that the CP content of A. occidentale (26.49%) was higher than F. thonningi (23.58%), M. indica (20.58%) and T. catappa (18.61%). Both ADF and NDF of A. occidentale (40.00 and 50.00) were as well higher than F. thonningi (20.00 and 40.00), M. indica (20.00 and 40.00) and T.catappa (20.00 and 42.00). Results from in vitro gas production however showed that T. catappa (23.67ml/DM) has a significantly higher (p<0.05) value than F.thonningi (20.67ml/DM), A. occidentale (16.67ml/DM), and M. indica(14.00ml/DM) at 72 hours of incubation. Methane gas production and in vitro gas production can be used to predict dry matter degradation and nutritive value of feedstuff for small ruminants. A. occidentale with the least methane gas production and highest crude protein (CP) content might have the most nutritive value among the browse plants investigated. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title="in vitro">in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=degradation" title=" degradation"> degradation</a>, <a href="https://publications.waset.org/abstracts/search?q=browse" title=" browse"> browse</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20production" title=" gas production"> gas production</a> </p> <a href="https://publications.waset.org/abstracts/44408/feed-value-of-selected-nigerian-browse-plants-chemical-composition-and-in-vitro-digestibility" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44408.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">357</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4539</span> Investigating the Successes of in vitro Embryogenesis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zelikha%20Labbani">Zelikha Labbani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The in vitro isolated microspore culture is the most powerful androgenic pathway to produce doubled haploid plants in the short time. To deviate a microspore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the microspore undergoes numerous changes at different levels, from overall morphology to gene expression. Induction of microspore embryogenesis not only implies the expression of an embryogenic program, but also a stress-related cellular response and a repression of the gametophytic program to revert the microspore to a totipotent status. As haploid single cells, microspore became a strategy to achieve various objectives particularly in genetic engineering. In this communication we would show the most recent advances in the producing haploid embryos via in vitro isolated microspore culture. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20isolated%20microspore%20culture" title="in vitro isolated microspore culture">in vitro isolated microspore culture</a>, <a href="https://publications.waset.org/abstracts/search?q=success" title=" success"> success</a>, <a href="https://publications.waset.org/abstracts/search?q=haploid%20cells" title=" haploid cells"> haploid cells</a>, <a href="https://publications.waset.org/abstracts/search?q=bioinformatics" title=" bioinformatics"> bioinformatics</a>, <a href="https://publications.waset.org/abstracts/search?q=biomedicine" title=" biomedicine"> biomedicine</a> </p> <a href="https://publications.waset.org/abstracts/9122/investigating-the-successes-of-in-vitro-embryogenesis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/9122.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">475</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4538</span> Design, Synthesis and in-vitro Antitumor Evaluation of Some Novel Substituted Quinazoline Derivatives</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adel%20S.%20El-Azab">Adel S. El-Azab</a>, <a href="https://publications.waset.org/abstracts/search?q=Alaa%20A.%20M.%20Abdel-Aziz"> Alaa A. M. Abdel-Aziz</a>, <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20A.%20Al-Suwaidan"> Ibrahim A. Al-Suwaidan</a>, <a href="https://publications.waset.org/abstracts/search?q=Amer%20M.%20Alanazi"> Amer M. Alanazi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A novel series of 2,3,6-trisubstitute quinazolinone were designed, synthesized, and evaluated for their in-vitro antitumor activity. 3 (Benzylideneamino)-6-chloro-2-p-tolylquinazolin-4(3H)-One, 2-[(4-oxo-3-phenethyl-3,4-dihydroquinazolin-2-yl)thio]-N-(3,4;5-trimethoxyphenyl) acetamide and 3-(3-benzyl-6-methyl-4-oxo-3, 4-dihydroquinazolin-2-ylthio)-N-(3,4,5-trimethoxyphenyl) propanamide have shown amazing broad spectrum antitumor activity with mean GI50; 15.8, 3.16, and 7.4 μM respectively compared to known Quinazoline Derivatives antitumor drug 5-FU mean GI50=22.6 μM. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=quinazoline%20derivatives" title="quinazoline derivatives">quinazoline derivatives</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20antitumor" title=" in vitro antitumor"> in vitro antitumor</a>, <a href="https://publications.waset.org/abstracts/search?q=synthesis" title=" synthesis"> synthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=5-FU" title=" 5-FU"> 5-FU</a>, <a href="https://publications.waset.org/abstracts/search?q=NCI" title=" NCI"> NCI</a> </p> <a href="https://publications.waset.org/abstracts/22501/design-synthesis-and-in-vitro-antitumor-evaluation-of-some-novel-substituted-quinazoline-derivatives" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22501.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">544</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4537</span> Cerium Salt Effect in 70s Bioactive Glass</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alessandra%20N.%20Santos">Alessandra N. Santos</a>, <a href="https://publications.waset.org/abstracts/search?q=Max%20P.%20Ferreira"> Max P. Ferreira</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexandra%20R.%20P.%20Silva"> Alexandra R. P. Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Agda%20A.%20R.%20de%20Oliveira"> Agda A. R. de Oliveira</a>, <a href="https://publications.waset.org/abstracts/search?q=Marivalda%20M.%20Pereira"> Marivalda M. Pereira</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The literature describes experiments, in which ceria nanoparticles in the bioactive glass significantly improve differentiation of stem cells into osteoblasts and increase production of collagen. It is not known whether this effect observed due to the presence of nanoceria can be also observed in the presence of cerium in the bioactive glass network. The effect of cerium into bioactive glasses using the sol–gel route is the focus of this work, with the goal to develop a material for tissue engineering with the potential to enhance osteogenesis. A bioactive glass composition based on 70% SiO2–30% CaO is produced with the addition of cerium. The analyses XRD, FTIR, SEM/EDS, BET/BJH, in vitro bioactivity test and the Cell viability assay were performed. The results show that cerium remains in the bioactive glass structure. The obtained material present in vitro bioactivity and promote the cell viability. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioactive%20glass" title="bioactive glass">bioactive glass</a>, <a href="https://publications.waset.org/abstracts/search?q=bioactivity" title=" bioactivity"> bioactivity</a>, <a href="https://publications.waset.org/abstracts/search?q=cerium%20salt" title=" cerium salt"> cerium salt</a>, <a href="https://publications.waset.org/abstracts/search?q=material%20characterization" title=" material characterization"> material characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=sol-gel%20method" title=" sol-gel method"> sol-gel method</a> </p> <a href="https://publications.waset.org/abstracts/102589/cerium-salt-effect-in-70s-bioactive-glass" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/102589.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">233</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4536</span> Effects of Rumen Protozoa and Nitrate on Fermentation and Methane Production </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20H.%20Nguyen">S. H. Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Li"> L. Li</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20S.%20Hegarty"> R. S. Hegarty</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Two experiments were conducted assessing the effects of presence or absence of rumen protozoa and dietary nitrate addition on rumen fermentation characteristics and methane production in Brahman heifers. The first experiment assessed changes in rumen fermentation pattern and in-vitro methane production post-refaunation and the second experiment investigated whether addition of nitrate to the incubation would give rise to methane mitigation additional to that contributed by defaunation. Ten Brahman heifers were progressively adapted to a diet containing coconut oil distillate 4.5% (COD) for 18 d and then all heifers were defaunated using sodium 1-(2-sulfonatooxyethoxy) dodecane (Empicol). After 15 d, the heifers were given a second dose of Empicol. Fifteen days after the second dosing, all heifers were allocated to defaunated or refaunated groups by stratified randomisation. On d 48, an oral dose of rumen fluid collected from unrelated faunated cattle was used to inoculate 5 heifers and form a refaunated group so that the effects of re-establishment of protozoa on fermentation characteristics could be investigated. Samples of rumen fluid collected from each animal using oesophageal intubation before feeding on d 48, 55, 62 and 69 were incubated for 23h in-vitro (experiment 1). On day 82, 2% of NO3 (as NaNO3) was included in in-vitro incubations (experiment 2) to test for additivity of NO3 and absence of protozoa effects on fermentation and methane production. It was concluded that increasing protozoal numbers were associated with increased methane production, with methane production rate significantly higher from refaunated heifers than from defaunated heifers 7, 14 and 21 d after refaunation. Concentration and proportions of major VFA, however, were not affected by protozoal treatments. There is scope for further reducing methane output through combining defaunation and dietary nitrate as the addition of nitrate in the defaunated heifers resulted in 86% reduction in methane production in-vitro. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=defaunation" title="defaunation">defaunation</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrate" title=" nitrate"> nitrate</a>, <a href="https://publications.waset.org/abstracts/search?q=fermentation" title=" fermentation"> fermentation</a>, <a href="https://publications.waset.org/abstracts/search?q=methane%20production" title=" methane production"> methane production</a> </p> <a href="https://publications.waset.org/abstracts/29114/effects-of-rumen-protozoa-and-nitrate-on-fermentation-and-methane-production" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29114.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">559</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4535</span> Effect of IGF-I on Ovine Oocytes Maturation and Subsequent Embryo Development following in Vitro Fertilization (IVF)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Babak%20Qasemi-Panahi">Babak Qasemi-Panahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Gholamali%20Moghaddam"> Gholamali Moghaddam</a>, <a href="https://publications.waset.org/abstracts/search?q=Seyed-Abbas%20Rafat"> Seyed-Abbas Rafat</a>, <a href="https://publications.waset.org/abstracts/search?q=Hossein%20Daghigh%20Kia"> Hossein Daghigh Kia</a>, <a href="https://publications.waset.org/abstracts/search?q=Mansoureh%20Movahedin"> Mansoureh Movahedin</a>, <a href="https://publications.waset.org/abstracts/search?q=Reza%20Hadavi"> Reza Hadavi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of this study was to determine the effects of IGF-I on ovine oocytes maturation and subsequent development of embryos derived from in vitro fertilization (IVF). In vitro maturation (IVM) of oocytes and in vitro culture (IVC) of embryos was conducted with or without 100 ng/mL IGF-1. In the IGF-I treated group, mean percentage of oocyte maturation was significantly higher than the control group (57.67 ± 3.04 versus 49.81 ± 3.04%, respectively, P < 0.05). However, in comparison with control group, there was no significant effect of IGF-1 on rates of cleavage, morula, and blastocyst formation (85% versus 84%; 63% versus 65%, and 40% to 39%, respectively). These data demonstrate that IGF-I has a positive effect on ovine oocyte maturation rate, but it has not the significant outcome on embryo development. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ovine" title="ovine">ovine</a>, <a href="https://publications.waset.org/abstracts/search?q=IGF-I" title=" IGF-I"> IGF-I</a>, <a href="https://publications.waset.org/abstracts/search?q=IVM" title=" IVM"> IVM</a>, <a href="https://publications.waset.org/abstracts/search?q=ICSI" title=" ICSI"> ICSI</a> </p> <a href="https://publications.waset.org/abstracts/21011/effect-of-igf-i-on-ovine-oocytes-maturation-and-subsequent-embryo-development-following-in-vitro-fertilization-ivf" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21011.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">688</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4534</span> Successes on in vitro Isolated Microspores Embryogenesis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zelikha%20Labbani">Zelikha Labbani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The In Vitro isolated micro spore culture is the most powerful androgenic pathway to produce doubled haploid plants in the short time. To deviate a micro spore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the micro spore undergoes numerous changes at different levels, from overall morphology to gene expression. Induction of micro spore embryogenesis not only implies the expression of an embryogenic program, but also a stress-related cellular response and a repression of the gametophytic program to revert the microspore to a totipotent status. As haploid single cells, micro spore became a strategy to achieve various objectives particularly in genetic engineering. In this study we would show the most recent advances in the producing haploid embryos via In Vitro isolated micro spore culture. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=haploid%20cells" title="haploid cells">haploid cells</a>, <a href="https://publications.waset.org/abstracts/search?q=In%20Vitro%20isolated%20microspore%20culture" title=" In Vitro isolated microspore culture"> In Vitro isolated microspore culture</a>, <a href="https://publications.waset.org/abstracts/search?q=success" title=" success"> success</a> </p> <a href="https://publications.waset.org/abstracts/26693/successes-on-in-vitro-isolated-microspores-embryogenesis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26693.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">615</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4533</span> On In vitro Durum Wheat Isolated Microspore Culture</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zelikha%20Labbani">Zelikha Labbani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Since its creation in 1964 by Guha and Maheshwari in India on Datura innoxia Mill, in vitro androgenesis has become the method of choice in the production of doubled haploid in many species. However, in durum wheat, the Doubled haploid plant breeding programs remained limited due to the low production of androgenetic embryos and converting them into fertile green plants. We describe here an efficient method for inducing embryos and regenerating green plants directly from isolated microspores of durum wheat. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=durum%20wheat" title="durum wheat">durum wheat</a>, <a href="https://publications.waset.org/abstracts/search?q=haploid%20embryos" title=" haploid embryos"> haploid embryos</a>, <a href="https://publications.waset.org/abstracts/search?q=on%20in%20vitro" title=" on in vitro"> on in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=pretreatment" title=" pretreatment"> pretreatment</a> </p> <a href="https://publications.waset.org/abstracts/44239/on-in-vitro-durum-wheat-isolated-microspore-culture" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44239.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">355</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4532</span> A Comparative Evaluation of Antioxidant Activity of in vivo and in vitro Raised Holarrhena antidysenterica Linn.</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gayatri%20Nahak">Gayatri Nahak</a>, <a href="https://publications.waset.org/abstracts/search?q=Satyajit%20Kanungo"> Satyajit Kanungo</a>, <a href="https://publications.waset.org/abstracts/search?q=Rajani%20Kanta%20Sahu"> Rajani Kanta Sahu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Holarrhena antidysenterica Linn. (Apocynaceae) is a typical Indian medicinal plant popularly known as “Indrajav”. Traditionally the plant has been considered a popular remedy for the treatment of dysentery, diarrhea, intestinal worms and the seeds of this plant are also used as an anti-diabetic remedy. In the present study axillary shoot multiplication, callus induction and shoot regeneration from callus culture were obtained on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators. Then in vivo and in vitro grown healthy plants were selected for study of antioxidant activity through DPPH and OH methods. Significantly higher antioxidant activity and phenol contents were observed in vitro raised plant in comparison to in vivo plants. The findings indicated the greater amount of phenolic compounds leads to more potent radical scavenging effect as shown in in vitro raised plant in comparison to in vivo plants which showed the ability to utilize tissue culture techniques towards development of desired bioactive metabolites from in vitro culture as an alternative way to avoid using endangered plants in pharmaceutical purposes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Holarrhena%20antidysenterica" title="Holarrhena antidysenterica">Holarrhena antidysenterica</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo" title=" in vivo"> in vivo</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a> </p> <a href="https://publications.waset.org/abstracts/16353/a-comparative-evaluation-of-antioxidant-activity-of-in-vivo-and-in-vitro-raised-holarrhena-antidysenterica-linn" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16353.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">510</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4531</span> Effects of Fenugreek Seed Extract on in vitro Maturation and Subsequent Development of Sheep Oocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20A.%20H.%20Barakat">Ibrahim A. H. Barakat</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20R.%20Al-Himaidi"> Ahmed R. Al-Himaidi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present study was conducted to determine the role and optimum concentration of fenugreek seed extract during in-vitro maturation on in-vitro maturation and developmental competence of Neaimi sheep oocytes following in-vitro fertilization. The Cumulus Oocyte Complexes (COCs) collected from sheep slaughterhouse ovaries were randomly divided into three groups, and they were matured for 24 hrs. in maturation medium containing fenugreek seed extract (0, 1 and 10 µg ml-1). Oocytes of a control group were matured in a medium containing 1 µg ml-1 estradiol 17β. After maturation, half of oocytes were fixed and stained for evaluation of nuclear maturation. The rest of oocytes were fertilized in vitro with fresh semen, then cultured for 9 days for the assessment of the developmental capacity of the oocytes. The results showed that the mean values of oocytes with expanded cumulus cells percentage were not significantly different among all groups (P < 0.05). But nuclear maturation rate of oocytes matured with 10 µg ml-1 fenugreek seed extract was significantly higher than that of the control group. The maturation rate and development to morula and blastocyst stage for oocytes matured at 10 µg ml-1 fenugreek seed extract was significantly higher than those matured at 1µg ml-1 of fenugreek seed extract and the control group. In conclusion, better maturation and developmental capacity rate to morula and blastocyst stage were obtained by the addition of 10 µg ml-1 fenugreek seed extract to maturation medium than addition of 1 µg ml-1 estradiol-17β (P < 0.05). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fenugreek%20seed%20extract" title="fenugreek seed extract">fenugreek seed extract</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20maturation" title=" in vitro maturation"> in vitro maturation</a>, <a href="https://publications.waset.org/abstracts/search?q=sheep%20oocytes" title=" sheep oocytes"> sheep oocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20fertilization" title=" in vitro fertilization"> in vitro fertilization</a>, <a href="https://publications.waset.org/abstracts/search?q=embryo%20development" title=" embryo development"> embryo development</a> </p> <a href="https://publications.waset.org/abstracts/3185/effects-of-fenugreek-seed-extract-on-in-vitro-maturation-and-subsequent-development-of-sheep-oocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3185.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">392</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4530</span> Development of an in vitro Fermentation Chicken Ileum Microbiota Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bello%20Gonzalez">Bello Gonzalez</a>, <a href="https://publications.waset.org/abstracts/search?q=Setten%20Van%20M."> Setten Van M.</a>, <a href="https://publications.waset.org/abstracts/search?q=Brouwer%20M."> Brouwer M.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The chicken small intestine represents a dynamic and complex organ in which the enzymatic digestion and absorption of nutrients take place. The development of an in vitro fermentation chicken small intestinal model could be used as an alternative to explore the interaction between the microbiota and nutrient metabolism and to enhance the efficacy of targeting interventions to improve animal health. In the present study we have developed an in vitro fermentation chicken ileum microbiota model for unrevealing the complex interaction of ileum microbial community under physiological conditions. A two-vessel continuous fermentation process simulating in real-time the physiological conditions of the ileum content (pH, temperature, microaerophilic/anoxic conditions, and peristaltic movements) has been standardized as a proof of concept. As inoculum, we use a pool of ileum microbial community obtained from chicken broilers at the age of day 14. The development and validation of the model provide insight into the initial characterization of the ileum microbial community and its dynamics over time-related to nutrient assimilation and fermentation. Samples can be collected at different time points and can be used to determine the microbial compositional structure, dynamics, and diversity over time. The results of studies using this in vitro model will serve as the foundation for the development of a whole small intestine in vitro fermentation chicken gastrointestinal model to complement our already established in vitro fermentation chicken caeca model. The insight gained from this model could provide us with some information about the nutritional strategies to restore and maintain chicken gut homeostasis. Moreover, the in vitro fermentation model will also allow us to study relationships between gut microbiota composition and its dynamics over time associated with nutrients, antimicrobial compounds, and disease modelling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=broilers" title="broilers">broilers</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20model" title=" in vitro model"> in vitro model</a>, <a href="https://publications.waset.org/abstracts/search?q=ileum%20microbiota" title=" ileum microbiota"> ileum microbiota</a>, <a href="https://publications.waset.org/abstracts/search?q=fermentation" title=" fermentation"> fermentation</a> </p> <a href="https://publications.waset.org/abstracts/185845/development-of-an-in-vitro-fermentation-chicken-ileum-microbiota-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/185845.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">57</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4529</span> In vitro and invivo Antioxidant Studies of Grewia crenata Leaves Extract in Albino Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20N.Ukwuani">A. N.Ukwuani</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20Abdulfatah"> A. K. Abdulfatah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> G. crenata is used locally for the treatment of fractured bones, wound healing and inflammatory conditions. In vitro and in vivo antioxidant activity of hydromethanolic extracts of the leaves of G. crenata were assessed. The phytochemical analysis shows the presence of phenols, flavonoids, saponins, cardiac glycosides and tannins. An in vitro quantitative analysis of phenols, flavonoids and tannins respectively were (164±1.20, 199±0.88 and 88.67±0.88 mg/100g FW). In vivo studies of hydromethanolic extract demonstrated a dose dependent increase in hepatic superoxide dismutase (1.14±0.14, 2.13±0.11, 2.55±0.11 U/mg Protein) with improvement in hepatic glutathione (6.98±0.42, 8.91±0.37, 11.07±0.46 µM/mg Protein) and Catalase (4.47±0.05, 6.24±0.02, 7.17±0.04 U/mg Protein) and Total protein (6.18±0.08, 6.69±0.18, 7.27±0.16 mg/ml) respectively at 100-300mg/kg body weight Grewia crenata leaves when compared to the control and standard drug. It can be concluded from the present findings of that G. crenata leaves possess antioxidant potential. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Grewia%20crenata" title="Grewia crenata">Grewia crenata</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=hydromethanolic%20extract" title=" hydromethanolic extract"> hydromethanolic extract</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo" title=" in vivo"> in vivo</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a> </p> <a href="https://publications.waset.org/abstracts/15568/in-vitro-and-invivo-antioxidant-studies-of-grewia-crenata-leaves-extract-in-albino-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15568.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">553</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4528</span> Biofertilization of Cucumber (Cucumis sativus L.) Using Trichoderma longibrachiatum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kehinde%20T.%20Kareem">Kehinde T. Kareem</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The need to increase the production of cucumber has led to the use of inorganic fertilizers. This chemical affects the ecological balance of nature by increasing the nitrogen and phosphorus contents of the soil. Surface runoffs into rivers and streams cause eutrophication which affects aquatic organisms as well as the consumers of aquatic animals. Therefore, this study was carried out in the screenhouse to investigate the use of a plant growth-promoting fungus; Trichoderma longibrachiatum for the growth promotion of conventional and in-vitro propagated Ashley and Marketmoor cucumber. Before planting of cucumber, spore suspension (108 cfu/ml) of Trichoderma longibrachiatum grown on Potato dextrose agar (PDA) was inoculated into the soil. Fruits were evaluated for the presence of Trichoderma longibrachiatum using a species-specific primer. Results revealed that the highest significant plant height produced by in-vitro propagated Ashley was 19 cm while the highest plant height of in-vitro propagated Marketmoor was 19.67 cm. The yield of the conventional propagated Ashley cucumber showed that the number of fruit/plant obtained from T. longibrachiatum-fertilized plants were significantly more than those of the control. The in-vitro Ashely had 7 fruits/plant while the control produced 4 fruits/plant. In-vitro Marketmoor had ten fruits/plant, and the control had a value of 4 fruits/plant. There were no traces of Trichoderma longibrachiatum genes in the harvested cucumber fruits. Therefore, the use of Trichoderma longibrachiatum as a plant growth-promoter is safe for human health as well as the environment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofertilizer" title="biofertilizer">biofertilizer</a>, <a href="https://publications.waset.org/abstracts/search?q=cucumber" title=" cucumber"> cucumber</a>, <a href="https://publications.waset.org/abstracts/search?q=genes" title=" genes"> genes</a>, <a href="https://publications.waset.org/abstracts/search?q=growth-promoter" title=" growth-promoter"> growth-promoter</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro" title=" in-vitro"> in-vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=propagation" title=" propagation"> propagation</a> </p> <a href="https://publications.waset.org/abstracts/56965/biofertilization-of-cucumber-cucumis-sativus-l-using-trichoderma-longibrachiatum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56965.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">244</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4527</span> Origanum vulgare as a Possible Modulator of Testicular Endocrine Function in Mice </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eva%20Tvrd%C3%A1">Eva Tvrdá</a>, <a href="https://publications.waset.org/abstracts/search?q=Barbora%20Babe%C4%8Dkov%C3%A1"> Barbora Babečková</a>, <a href="https://publications.waset.org/abstracts/search?q=Michal%20%C4%8Eura%C4%8Dka"> Michal Ďuračka</a>, <a href="https://publications.waset.org/abstracts/search?q=R%C3%B3bert%20Kirchner"> Róbert Kirchner</a>, <a href="https://publications.waset.org/abstracts/search?q=J%C3%BAlius%20%C3%81rvay"> Július Árvay</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study was designed to assess the <em>in vitro</em> effects of <em>Origanum vulgare</em> L. (oregano) extract on the testicular steroidogenesis. We focused on identifying major biomolecules present in the oregano extract, as well as to investigate its <em>in vitro</em> impact on the secretion of cholesterol, testosterone, dehydroepiandrosterone and androstenedione by murine testicular fragments. The extract was subjected to high performance liquid chromatography (HPLC) which identified cyranosid, daidzein, thymol, rosmarinic and trans-caffeic acid among the predominant biochemical components of oregano. For the <em>in vitro</em> experiments, testicular fragments from 20 sexually mature Institute of Cancer Research (ICR) mice were incubated in the absence (control group) or presence of the oregano extract at selected concentrations (10, 100 and 1000 μg/mL) for 24 h. Cholesterol levels were quantified using photometry and the hormones were assessed by ELISA (Enzyme-Linked Immunosorbent Assay). Our data revealed that the release of cholesterol and androstenedione (but not dehydroepiandrosterone and testosterone) by the testicular fragments was significantly impacted by the oregano extract in a dose-dependent fashion. Supplementation of the extract resulted in a significant decline of cholesterol (P < 0.05 in case of 100 μg/mL; P < 0.01 with respect 100 μg/mL extract), as well as androstenedione (P < 0.01 with respect to 100 and 1000 μg/mL extract). Our results suggest that the biomolecules present in <em>Origanum vulgare</em> L. could exhibit a dose-dependent impact on the secretion of male steroids, playing a role in the regulation of testicular steroidogenesis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mice" title="mice">mice</a>, <a href="https://publications.waset.org/abstracts/search?q=Origanum%20vulgare%20L." title=" Origanum vulgare L."> Origanum vulgare L.</a>, <a href="https://publications.waset.org/abstracts/search?q=steroidogenesis" title=" steroidogenesis"> steroidogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=testes" title=" testes "> testes </a> </p> <a href="https://publications.waset.org/abstracts/108962/origanum-vulgare-as-a-possible-modulator-of-testicular-endocrine-function-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/108962.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">167</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4526</span> Comparative and Combined Toxicity of NiO and Mn₃O₄ Nanoparticles as Assessed in vitro and in vivo</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ilzira%20A.%20Minigalieva">Ilzira A. Minigalieva</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatiana%20V.%20Bushueva"> Tatiana V. Bushueva</a>, <a href="https://publications.waset.org/abstracts/search?q=Eleonore%20Frohlich"> Eleonore Frohlich</a>, <a href="https://publications.waset.org/abstracts/search?q=Vladimir%20Panov"> Vladimir Panov</a>, <a href="https://publications.waset.org/abstracts/search?q=Ekaterina%20Shishkina"> Ekaterina Shishkina</a>, <a href="https://publications.waset.org/abstracts/search?q=Boris%20A.%20Katsnelson"> Boris A. Katsnelson</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The overwhelming majority of the experimental studies in the field of metal nanotoxicology have been performed on cultures of established cell lines, with very few researchers focusing on animal experiments, while a juxtaposition of conclusions inferred from these two types of research is blatantly lacking. The least studied aspect of this problem relates to characterizing and predicting the combined toxicity of metallic nanoparticles. Methods: Comparative and combined toxic effects of purposefully prepared spherical NiO and Mn₃O₄ nanoparticles (mean diameters 16.7 ± 8.2 nm and 18.4 ± 5.4 nm respectively) were estimated on cultures of human cell lines: MRC-5 fibroblasts, THP-1 monocytes, SY-SY5Y neuroblastoma cells, as well as on the latter two lines differentiated to macrophages and neurons, respectively. The combined cytotoxicity was mathematically modeled using the response surface methodology. Results: The comparative assessment of the studied NPs unspecific toxicity previously obtained in vivo was satisfactorily reproduced by the present in vitro tests. However, with respect to manganese-specific brain damage which had been demonstrated by us in animal experiment with the same NPs, the testing on neuronall cell culture showed only a certain enhancing effect of Mn₃O₄-NPs on the toxic action of NiO-NPs, while the role of the latter prevailed. Conclusion: From the point of view of the preventive toxicology, the experimental modeling of metallic NPs combined toxicity on cell cultures can give non-reliable predictions of the in vivo action’s effects. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=manganese%20oxide" title="manganese oxide">manganese oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=nickel%20oxide" title=" nickel oxide"> nickel oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20toxicity" title=" in vitro toxicity"> in vitro toxicity</a> </p> <a href="https://publications.waset.org/abstracts/82038/comparative-and-combined-toxicity-of-nio-and-mn3o4-nanoparticles-as-assessed-in-vitro-and-in-vivo" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/82038.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">297</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4525</span> Crude Glycerol Affects Canine Spermatoa Motility: Computer Assister Semen Analysis in Vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20Massanyi">P. Massanyi</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Kichi"> L. Kichi</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Slanina"> T. Slanina</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Kolesar"> E. Kolesar</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Danko"> J. Danko</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Lukac"> N. Lukac</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Tvrda"> E. Tvrda</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Stawarz"> R. Stawarz</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Kolesarova"> A. Kolesarova </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Target of this study was the analysis of the impact of crude glycerol on canine spermatozoa motility, morphology, viability, and membrane integrity. Experiments were realized in vitro. In the study, semen from 5 large dog breeds was used. They were typical representatives of large breeds, coming from healthy rearing, regularly vaccinated and integrated to the further breeding. Semen collections were realized at the owners of animals and in the veterinary clinic. Subsequently the experiments were realized at the Department of Animal Physiology of the SUA in Nitra. The spermatozoa motility was evaluated using CASA analyzer (SpermVisionTM, Minitub, Germany) at the temperature 5 and 37°C for 5 hours. In the study, 13 motility parameters were evaluated. Generally, crude glycerol has generally negative effect on spermatozoa motility. Morphological analysis was realized using Hancock staining and the preparations were evaluated at magnification 1000x using classification tables of morphologically changed spermatozoa. Data clearly detected the highest number of morphologically changed spermatozoa in the experimental groups (know twisted tails, tail torso and tail coiling). For acrosome alterations swelled acrosomes, removed acrosomes and acrosomes with undulated membrane were detected. In this study also the effect of crude glycerol on spermatozoa membrane integrity were analyzed. The highest crude glycerol concentration significantly affects spermatozoa integrity. Results of this study show that crude glycerol has effect of spermatozoa motility, viability, and membrane integrity. Detected changes are related to crude glycerol concentration, temperature, as well as time of incubation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dog" title="dog">dog</a>, <a href="https://publications.waset.org/abstracts/search?q=semen" title=" semen"> semen</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatozoa" title=" spermatozoa"> spermatozoa</a>, <a href="https://publications.waset.org/abstracts/search?q=acrosome" title=" acrosome"> acrosome</a>, <a href="https://publications.waset.org/abstracts/search?q=glycerol" title=" glycerol"> glycerol</a>, <a href="https://publications.waset.org/abstracts/search?q=CASA" title=" CASA"> CASA</a>, <a href="https://publications.waset.org/abstracts/search?q=viability" title=" viability"> viability</a> </p> <a href="https://publications.waset.org/abstracts/35012/crude-glycerol-affects-canine-spermatoa-motility-computer-assister-semen-analysis-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35012.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">319</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4524</span> Selection of Developmental Stages of Bovine in vitro-Derived Blastocysts Prior to Vitrification and Embryo Transfer: Implications for Cattle Breeding Programs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Van%20Huong%20Do">Van Huong Do</a>, <a href="https://publications.waset.org/abstracts/search?q=Simon%20Walton"> Simon Walton</a>, <a href="https://publications.waset.org/abstracts/search?q=German%20Amaya"> German Amaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Madeline%20Batsiokis"> Madeline Batsiokis</a>, <a href="https://publications.waset.org/abstracts/search?q=Sally%20Catt"> Sally Catt</a>, <a href="https://publications.waset.org/abstracts/search?q=Andrew%20Taylor-Robinson"> Andrew Taylor-Robinson</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Identification of the most suitable stages of bovine in vitro-derived blastocysts (early, expanded and hatching) prior to vitrification is a straightforward process that facilitates the decision as to which blastocyst stage to use for transfer of fresh and vitrified embryos. Research on in vitro evaluation of suitable stages has shown that the more advanced developmental stage of blastocysts is recommended for fresh embryo transfer while the earlier stage is proposed for embryo transfer following vitrification. There is, however, limited information on blastocyst stages using in vivo assessment. Hence, the aim of the present study was to determine the optimal stage of a blastocyst for vitrification and embryo transfer through a two-step procedure of embryo transfer followed by pregnancy testing at 35, 60 and 90 days of pregnancy. 410 good quality oocytes aspirated by the ovum pick-up technique from 8 donor cows were subjected to in vitro embryo production, vitrification and embryo transfer. Good quality embryos were selected, subjected to vitrification and embryo transfer. Subsequently, 77 vitrified embryos at different blastocyst stages were transferred to synchronised recipient cows. The overall cleavage and blastocyst rates of oocytes were 68.8% and 41.7%, respectively. In addition, the fertility and blastocyst production of 6 bulls used for in vitro fertilization was examined and shown to be statistically different (P<0.05). Results of ongoing pregnancy trials conducted at 35 days, 60 days and 90 days will be discussed. However, preliminary data indicate that individual bulls demonstrate distinctly different fertility performance in vitro. Findings from conception rates would provide a useful tool to aid selection of bovine in vitro-derived embryos for vitrification and embryo transfer in commercial settings. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=blastocyst" title="blastocyst">blastocyst</a>, <a href="https://publications.waset.org/abstracts/search?q=embryo%20transfer" title=" embryo transfer"> embryo transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro-derived%20embryos" title=" in vitro-derived embryos"> in vitro-derived embryos</a>, <a href="https://publications.waset.org/abstracts/search?q=ovum%20pick-up" title=" ovum pick-up"> ovum pick-up</a>, <a href="https://publications.waset.org/abstracts/search?q=vitrification" title=" vitrification"> vitrification</a> </p> <a href="https://publications.waset.org/abstracts/78837/selection-of-developmental-stages-of-bovine-in-vitro-derived-blastocysts-prior-to-vitrification-and-embryo-transfer-implications-for-cattle-breeding-programs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78837.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">307</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4523</span> The Evaluation of Substitution of Acacia villosa in Ruminants Ration</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hadriana%20Bansi">Hadriana Bansi</a>, <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20Wina"> Elizabeth Wina</a>, <a href="https://publications.waset.org/abstracts/search?q=Toto%20Toharmat"> Toto Toharmat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acacia villosa is thornless shrub legume which contents high crude protein. However, the utilization of A. villosa as ruminant feed is limited by its secondary compounds. The aim of this article is to find out the maximum of substitution A. villosa in sheep ration. The nutritional evaluation consisted of in vitro two stages, in vivo, and in vitro gas production trials. The secondary compounds of A. villosa also were analyzed. Evaluating digestibility of increasing level of substitution A. villosa replacing Pennisetum purpureum was using in vitro two stages. The substitution of 30% A. villosa was compared to 100% P. purpureum by in vitro gas production technique and in vivo digestibility. The results of two stages in vitro showed that total phenol, condensed tannin, and non-protein amino acid (NPAA) were high. Substitution 15% A. villosa reached the highest digestibility for both dry matter (DM) and crude protein (CP) which were 67% and 86% respectively, but it was shown that DM and CP digestibility of substitution 30% of A. villosa was still high which were 61.82% and 75-67% respectively. The pattern of gas production showed that first 8 hours total gas production substitution of 30% A. villosa was higher than 100% P. purpureum and declined after 10 hours incubation. In vivo trials showed that substitution of 30% A. villosa significantly increased CP intake, CP digestibility, and nitrogen retention. It can be concluded that substitution A. villosa until 30% still gave the good impact even though it has high secondary compounds. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acacia%20villosa" title="Acacia villosa">Acacia villosa</a>, <a href="https://publications.waset.org/abstracts/search?q=digestibility" title=" digestibility"> digestibility</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20production" title=" gas production"> gas production</a>, <a href="https://publications.waset.org/abstracts/search?q=secondary%20compounds" title=" secondary compounds"> secondary compounds</a> </p> <a href="https://publications.waset.org/abstracts/96358/the-evaluation-of-substitution-of-acacia-villosa-in-ruminants-ration" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96358.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">163</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4522</span> The Role of the STAT3 Signaling for Melatonergic Synthetic Pathway in the Rat Pineal Gland</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Simona%20Moravcova">Simona Moravcova</a>, <a href="https://publications.waset.org/abstracts/search?q=Jiri%20Novotny"> Jiri Novotny</a>, <a href="https://publications.waset.org/abstracts/search?q=Zdenka%20Bendova"> Zdenka Bendova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The pineal gland of the vertebrate brain is a circumventricular organ which serves as a major neuroendocrine gland with the primary function of rhythmic secretion of neurohormone melatonin under the control of the hypothalamic suprachiasmatic nucleus (SCN). Soon after the onset of the darkness, the activity of the key rate-limiting enzyme for melatonin synthesis, arylalkylamine N-acetyltransferase (AANAT), raises due to the increased release of norepinephrine from sympathetic neurons terminating on the parenchymal cells where it binds to β-adrenergic receptors. Melatonin codes the length of the night, and it is well recognized for its anti-inflammatory effects. However, to our knowledge, less is known about the effect of the immune system on the melatonin biosynthesis and the precise role of the STAT3 in the signaling pathway leading to the expression of AANAT. Lipopolysaccharide (LPS) is the essential component in the outer surface membrane of gram-negative bacteria and acts as a strong stimulator of natural and innate immunity. STAT3 acts as an important factor in immune response. Here we investigated the effect of LPS on the components of the melatonergic synthetic pathway in the pineal gland. The experiments were performed both in vivo and in vitro. The changes in AANAT activity were determined by radioenzymatic assay. PCR analyses were carried out to detect aa-nat, icer, spi-3 and stat3 gene expression. From our results, it is apparent that the high basal level of phosphorylated forms of STAT3 can be elevated after systemic as well as in vitro administration of LPS. Our experiments have shown that LPS reduces melatonin synthesis, nevertheless, the activity of AANAT was increased. Moreover, the basal level of phosphorylated STAT3 counteracts β-adrenergic receptor-mediated aa-nat gene expression and sustains its own and spi-3 gene expression. In conclusion, LPS can affect immunomodulators such as melatonin in the pineal gland. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=AANAT" title="AANAT">AANAT</a>, <a href="https://publications.waset.org/abstracts/search?q=lipopolysaccharide" title=" lipopolysaccharide"> lipopolysaccharide</a>, <a href="https://publications.waset.org/abstracts/search?q=pineal%20gland" title=" pineal gland"> pineal gland</a>, <a href="https://publications.waset.org/abstracts/search?q=rat" title=" rat"> rat</a>, <a href="https://publications.waset.org/abstracts/search?q=STAT3" title=" STAT3"> STAT3</a> </p> <a href="https://publications.waset.org/abstracts/99049/the-role-of-the-stat3-signaling-for-melatonergic-synthetic-pathway-in-the-rat-pineal-gland" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99049.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">169</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4521</span> Indenyl and Allyl Palladates: Synthesis, Bonding, and Anticancer Activity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20Scattolin">T. Scattolin</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Cavarzerani"> E. Cavarzerani</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Visentin"> F. Visentin</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Rizzolio"> F. Rizzolio</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Organopalladium compounds have recently attracted attention for their high stability even under physiological conditions and, above all, for their remarkable in vitro cytotoxicity towards cisplatin-resistant cell lines. Among the organopalladium derivatives, those bearing at least one N-heterocyclic carbene ligand (NHC) and the Pd(II)-η³-allyl fragment have exhibited IC₅₀ values in the micro and sub-micromolar range towards several cancer cell lines in vitro and in some cases selectivity towards cancerous vs. non-tumorigenic cells. Herein, a selection of allyl and indenyl palladates were synthesized using a solvent-free method consisting of grinding the corresponding palladium precursors with different saturated and unsaturated azolium salts. All compounds have been fully characterized by NMR, XRD and elemental analyses. The intramolecular H, Cl interaction has been elucidated and quantified using the Voronoi Deformation Density scheme. Most of the complexes showed excellent cytotoxicity towards ovarian cancer cell lines, with I₅₀ values comparable to or even lower than cisplatin. Interestingly, the potent anticancer activity was also confirmed in a high-serous ovarian cancer (HGSOC) patient-derived tumoroid, with a clear superiority of this class of compounds over classical platinum-based agents. Finally, preliminary enzyme inhibition studies of the synthesized palladate complexes against the model TrxR show that the compounds have high activity comparable to or even higher than auranofin and classical Au(I) NHC complexes. Based on such promising data, further in vitro and in vivo experiments and in-depth mechanistic studies are ongoing in our laboratories. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anticancer%20activity" title="anticancer activity">anticancer activity</a>, <a href="https://publications.waset.org/abstracts/search?q=palladium%20complexes" title=" palladium complexes"> palladium complexes</a>, <a href="https://publications.waset.org/abstracts/search?q=organoids" title=" organoids"> organoids</a>, <a href="https://publications.waset.org/abstracts/search?q=indenyl%20and%20allyl%20ligands" title=" indenyl and allyl ligands"> indenyl and allyl ligands</a> </p> <a href="https://publications.waset.org/abstracts/148846/indenyl-and-allyl-palladates-synthesis-bonding-and-anticancer-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/148846.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">94</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4520</span> In-vitro Antioxidant Activity of Two Selected Herbal Medicines</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Vinotha">S. Vinotha</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Thabrew"> I. Thabrew</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Sri%20Ranjani"> S. Sri Ranjani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hot aqueous and methanol extracts of the two selected herbal medicines such are Vellarugu Chooranam (V.C) and Amukkirai Chooranam (A.C) were examined for total phenolic and flavonoid contents and in-vitro antioxidant activity using four different methods. The total phenolic and flavonoid contents in methanol extract of V.C were found to be higher (44.41±1.26 mg GAE⁄g; 174.44±9.32 mg QE⁄g) than in the methanol extract of A.C (20.56±0.67 mg GAE⁄g;7.21±0.85 mg QE⁄g). Hot methanol and aqueous extracts of both medicines showed low antioxidant activity in DPPH, ABTS, and FRAP methods and Iron chelating activity not found at highest possible concentration. V.C contains higher concentrations of total phenolic and flavonoid contents than A.C and can also exert greater antioxidant activity than A.C, although the activities demonstrated were lower than the positive control Trolox. The in-vitro antioxidant activity was not related with the total phenolic and flavonoid contents of the methanol and aqueous extracts of both herbal medicines (A.C and V.C). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=activity" title="activity">activity</a>, <a href="https://publications.waset.org/abstracts/search?q=different%20extracts" title=" different extracts"> different extracts</a>, <a href="https://publications.waset.org/abstracts/search?q=herbal%20medicines" title=" herbal medicines"> herbal medicines</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro%20antioxidant" title=" in-vitro antioxidant"> in-vitro antioxidant</a> </p> <a href="https://publications.waset.org/abstracts/16823/in-vitro-antioxidant-activity-of-two-selected-herbal-medicines" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16823.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">405</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4519</span> Development of Positron Emission Tomography (PET) Tracers for the in-Vivo Imaging of α-Synuclein Aggregates in α-Synucleinopathies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bright%20Chukwunwike%20Uzuegbunam">Bright Chukwunwike Uzuegbunam</a>, <a href="https://publications.waset.org/abstracts/search?q=Wojciech%20%20Paslawski"> Wojciech Paslawski</a>, <a href="https://publications.waset.org/abstracts/search?q=Hans%20Agren"> Hans Agren</a>, <a href="https://publications.waset.org/abstracts/search?q=Christer%20Halldin"> Christer Halldin</a>, <a href="https://publications.waset.org/abstracts/search?q=Wolfgang%20Weber"> Wolfgang Weber</a>, <a href="https://publications.waset.org/abstracts/search?q=Markus%20Luster"> Markus Luster</a>, <a href="https://publications.waset.org/abstracts/search?q=Thomas%20Arzberger"> Thomas Arzberger</a>, <a href="https://publications.waset.org/abstracts/search?q=Behrooz%20Hooshyar%20Yousefi"> Behrooz Hooshyar Yousefi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> There is a need to develop a PET tracer that will enable to diagnosis and track the progression of Alpha-synucleinopathies (Parkinson’s disease [PD], dementia with Lewy bodies [DLB], multiple system atrophy [MSA]) in living subjects over time. Alpha-synuclein aggregates (a-syn), which are present in all the stages of disease progression, for instance, in PD, are a suitable target for in vivo PET imaging. For this reason, we have developed some promising a-syn tracers based on a disarylbisthiazole (DABTA) scaffold. The precursors are synthesized via a modified Hantzsch thiazole synthesis. The precursors were then radiolabeled via one- or two-step radiofluorination methods. The ligands were initially screened using a combination of molecular dynamics and quantum/molecular mechanics approaches in order to calculate the binding affinity to a-syn (in silico binding experiments). Experimental in vitro binding assays were also performed. The ligands were further screened in other experiments such as log D, in vitro plasma protein binding & plasma stability, biodistribution & brain metabolite analyses in healthy mice. Radiochemical yields were up to 30% - 72% in some cases. Molecular docking revealed possible binding sites in a-syn and also the free energy of binding to those sites (-28.9 - -66.9 kcal/mol), which correlated to the high binding affinity of the DABTAs to a-syn (Ki as low as 0.5 nM) and selectivity (> 100-fold) over Aβ and tau, which usually co-exist with a-synin some pathologies. The log D values range from 2.88 - 2.34, which correlated with free-protein fraction of 0.28% - 0.5%. Biodistribution experiments revealed that the tracers are taken up (5.6 %ID/g - 7.3 %ID/g) in the brain at 5 min (post-injection) p.i., and cleared out (values as low as 0.39 %ID/g were obtained at 120 min p.i. Analyses of the mice brain 20 min p.i. Revealed almost no radiometabolites in the brain in most cases. It can be concluded that in silico study presents a new venue for the rational development of radioligands with suitable features. The results obtained so far are promising and encourage us to further validate the DABTAs in autoradiography, immunohistochemistry, and in vivo imaging in non-human primates and humans. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alpha-synuclein%20aggregates" title="alpha-synuclein aggregates">alpha-synuclein aggregates</a>, <a href="https://publications.waset.org/abstracts/search?q=alpha-synucleinopathies" title=" alpha-synucleinopathies"> alpha-synucleinopathies</a>, <a href="https://publications.waset.org/abstracts/search?q=PET%20imaging" title=" PET imaging"> PET imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=tracer%20development" title=" tracer development"> tracer development</a> </p> <a href="https://publications.waset.org/abstracts/138870/development-of-positron-emission-tomography-pet-tracers-for-the-in-vivo-imaging-of-a-synuclein-aggregates-in-a-synucleinopathies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/138870.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">235</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4518</span> Growth of Albizia in vitro: Endophytic Fungi as Plant Growth Promote of Albizia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reine%20Suci%20Wulandari">Reine Suci Wulandari</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosa%20Suryantini"> Rosa Suryantini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Albizia (Paraserianthes falcataria) is a woody plant species that has a high economic value and multifunctional. Albizia is important timber, medicinal plants and can also be used as a plant to rehabilitate critical lands. The demand value of Albizia is increased so that the large quantities and high quality of seeds are required. In vitro propagation techniques are seed propagation that can produce more seeds and quality in a short time. In vitro cultures require growth regulators that can be obtained from biological agents such as endophytic fungi. Endophytic fungi are micro fungi that colonize live plant tissue without producing symptoms or other negative effects on host plants and increase plant growth. The purposes of this research were to isolate and identify endophytic fungi isolated from the root of Albizia and to study the effect of endophytic fungus on the growth of Albizia in vitro. The methods were root isolation, endophytic fungal identification, and inoculation of endophytic fungi to Albizia plants in vitro. Endophytic fungus isolates were grown on PDA media before being inoculated with Albizia sprouts. Incubation is done for 4 (four) weeks. The observed growth parameters were live explant percentage, percentage of explant shoot, and percentage of explant rooted. The results of the research showed that 6 (six) endophytic fungal isolates obtained from the root of Albizia, namely Aspergillus sp., Verticillium sp, Penicillium sp., Trichoderma sp., Fusarium sp., and Acremonium sp. Statistical analysis found that Trichoderma sp. and Fusarium sp. affect in vitro growth of Albizia. Endophytic fungi from the results of this research were potential as plant growth promoting. It can be applied to increase productivity either through increased plant growth and increased endurance of Albizia seedlings to pests and diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Albizia" title="Albizia">Albizia</a>, <a href="https://publications.waset.org/abstracts/search?q=endophytic%20fungi" title=" endophytic fungi"> endophytic fungi</a>, <a href="https://publications.waset.org/abstracts/search?q=propagation" title=" propagation"> propagation</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a> </p> <a href="https://publications.waset.org/abstracts/74725/growth-of-albizia-in-vitro-endophytic-fungi-as-plant-growth-promote-of-albizia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74725.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">266</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4517</span> Combining Experiments and Surveys to Understand the Pinterest User Experience</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jolie%20M.%20Martin">Jolie M. Martin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Running experiments while logging detailed user actions has become the standard way of testing product features at Pinterest, as at many other Internet companies. While this technique offers plenty of statistical power to assess the effects of product changes on behavioral metrics, it does not often give us much insight into why users respond the way they do. By combining at-scale experiments with smaller surveys of users in each experimental condition, we have developed a unique approach for measuring the impact of our product and communication treatments on user sentiment, attitudes, and comprehension. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=experiments" title="experiments">experiments</a>, <a href="https://publications.waset.org/abstracts/search?q=methodology" title=" methodology"> methodology</a>, <a href="https://publications.waset.org/abstracts/search?q=surveys" title=" surveys"> surveys</a>, <a href="https://publications.waset.org/abstracts/search?q=user%20experience" title=" user experience"> user experience</a> </p> <a href="https://publications.waset.org/abstracts/48415/combining-experiments-and-surveys-to-understand-the-pinterest-user-experience" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/48415.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">312</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4516</span> The Effect of Ethylene Glycol on Cryopreserved Bovine Oocytes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sri%20Wahjuningsih">Sri Wahjuningsih</a>, <a href="https://publications.waset.org/abstracts/search?q=Nur%20Ihsan"> Nur Ihsan</a>, <a href="https://publications.waset.org/abstracts/search?q=Hadiah"> Hadiah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the embryo transfer program, to address the limited production of embryos in vivo, in vitro embryo production has become an alternative approach that is relatively inexpensive. One potential source of embryos that can be developed is to use immature oocytes then conducted in vitro maturation and in vitro fertilization. However, obstacles encountered were oocyte viability mammals have very limited that it cannot be stored for a long time, so we need oocyte cryopreservation. The research was conducted to know the optimal concentration use of ethylene glycol as a cryoprotectant on oocytes freezing.Material use in this research was immature oocytes; taken from abbatoir which was aspirated from follicle with diameter 2-6 mm. Concentration ethylen glycol used were 0,5 M, I M, 1,5 M and 2M. The freezing method used was conventional method combined with a five-step protocol washing oocytes from cryoprotectant after thawing. The result showed that concentration ethylen glycol have the significant effect (P<0.05) on oocytes quality after thawing and in vitro maturation. It was concluded that concentration 1,5 M was the best concentration for freezing oocytes using conventional method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bovine" title="bovine">bovine</a>, <a href="https://publications.waset.org/abstracts/search?q=conventional%20freezing" title=" conventional freezing"> conventional freezing</a>, <a href="https://publications.waset.org/abstracts/search?q=ethylen%20glycol" title=" ethylen glycol"> ethylen glycol</a>, <a href="https://publications.waset.org/abstracts/search?q=oocytes" title=" oocytes"> oocytes</a> </p> <a href="https://publications.waset.org/abstracts/39761/the-effect-of-ethylene-glycol-on-cryopreserved-bovine-oocytes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39761.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge 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