CINXE.COM

<!DOCTYPE html> <html lang="en" dir="ltr"> <head>  <script async src="https://www.googletagmanager.com/gtag/js?id=G-P63WKM1TM1"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-P63WKM1TM1'); </script>  <script type="text/javascript" > (function(m,e,t,r,i,k,a){m[i]=m[i]||function(){(m[i].a=m[i].a||[]).push(arguments)}; m[i].l=1*new Date(); for (var j = 0; j < document.scripts.length; j++) {if (document.scripts[j].src === r) { return; }} k=e.createElement(t),a=e.getElementsByTagName(t)[0],k.async=1,k.src=r,a.parentNode.insertBefore(k,a)}) (window, document, "script", "https://mc.yandex.ru/metrika/tag.js", "ym"); ym(55165297, "init", { clickmap:false, trackLinks:true, accurateTrackBounce:true, webvisor:false }); </script> <noscript><div><img src="https://mc.yandex.ru/watch/55165297" style="position:absolute; left:-9999px;" alt="" /></div></noscript>    <title>Search results for: in vitro experiments</title> <meta name="description" content="Search results for: in vitro experiments"> <meta name="keywords" content="in vitro experiments"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="in vitro experiments" name="q" aria-label="Search"> <button class="btn btn-light my-2 my-sm-0" type="submit"><i class="fas fa-search"></i></button> </form> </div> <div class="collapse navbar-collapse mt-1" id="navbarMenu"> <ul class="navbar-nav ml-auto align-items-center" id="mainNavMenu"> <li class="nav-item"> <a class="nav-link" href="https://waset.org/conferences" title="Conferences in 2024/2025/2026">Conferences</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/disciplines" title="Disciplines">Disciplines</a> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/committees" rel="nofollow">Committees</a> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdownPublications" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Publications </a> <div class="dropdown-menu" aria-labelledby="navbarDropdownPublications"> <a class="dropdown-item" href="https://publications.waset.org/abstracts">Abstracts</a> <a class="dropdown-item" href="https://publications.waset.org">Periodicals</a> <a class="dropdown-item" href="https://publications.waset.org/archive">Archive</a> </div> </li> <li class="nav-item"> <a class="nav-link" href="https://waset.org/page/support" title="Support">Support</a> </li> </ul> </div> </div> </nav> </div> </header> <main> <div class="container mt-4"> <div class="row"> <div class="col-md-9 mx-auto"> <form method="get" action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="in vitro experiments"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4545</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: in vitro experiments</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4335</span> Study of the in vivo and in vitro Antioxidant Activity of the Methanol Extract from the Roots of the Barks of Zizyphus lotus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Djemai%20Zoughlache%20Soumia">Djemai Zoughlache Soumia</a>, <a href="https://publications.waset.org/abstracts/search?q=Yahia%20Mouloud"> Yahia Mouloud</a>, <a href="https://publications.waset.org/abstracts/search?q=Lekbir%20Adel"> Lekbir Adel</a>, <a href="https://publications.waset.org/abstracts/search?q=Meslem%20Meriem"> Meslem Meriem</a>, <a href="https://publications.waset.org/abstracts/search?q=Maouchi%20Madiha"> Maouchi Madiha</a>, <a href="https://publications.waset.org/abstracts/search?q=Bahi%20Ahlem"> Bahi Ahlem</a>, <a href="https://publications.waset.org/abstracts/search?q=Benbia%20Souhila"> Benbia Souhila</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Natural extracts is known for their contents of biologically active molecules. In this context, we attempted to evaluate the antioxidant activity of the methanolic extract prepared from the bark of the roots of Zizyphus lotus. The quantitative analysis based on the dosage, phenolic compounds, flavonoids and tannins provided following values: 0.39 ± 0.007 ug EAG/mg of extract for phenolic compounds, 0.05 ± 0.02ug EQ/mg extract for flavonoids and 0.0025 ± 7.071 E-4 ECT ug/mg extract for tannins. The study of the antioxidant activity by the DPPH test in vitro showed a powerful antiradical power with an IC50 = 8,8 ug/ml. For the DPPH test in vivo we used two rats lots, one lot with a dose of 200 mg/kg of the methanol extract and a control lot. We found a significant difference in antiradical activity with p < 0.05. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zizyphus%20lotus" title="Zizyphus lotus">Zizyphus lotus</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH" title=" DPPH"> DPPH</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20compounds" title=" phenolic compounds"> phenolic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=flavonoids" title=" flavonoids"> flavonoids</a>, <a href="https://publications.waset.org/abstracts/search?q=tannins" title=" tannins"> tannins</a> </p> <a href="https://publications.waset.org/abstracts/5758/study-of-the-in-vivo-and-in-vitro-antioxidant-activity-of-the-methanol-extract-from-the-roots-of-the-barks-of-zizyphus-lotus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5758.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">509</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4334</span> Role of Long Noncoding RNA HULC on Colorectal Carcinoma Progression through Epigenetically Repressing NKD2 Expression</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shu-Jun%20Li">Shu-Jun Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheng-Cao%20Sun"> Cheng-Cao Sun</a>, <a href="https://publications.waset.org/abstracts/search?q=De-Jia%20Li"> De-Jia Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recently, long noncoding RNAs (lncRNAs) have been emerged as crucial regulators of human diseases and prognostic markers in numerous of cancers, including colorectal carcinoma (CRC). Here, we identified an oncogenetic lncRNA HULC, which may promote colorectal tumorigenesis. HULC has been found to be up-regulated and acts as oncogene in gastric cancer and hepatocellular carcinoma, but its expression pattern, biological function and underlying mechanism in CRC is still undetermined. Here, we reported that HULC expression is also over-expressed in CRC, and its increased level is associated with poor prognosis and shorter survival. Knockdown of HULC impaired CRC cells proliferation, migration and invasion, facilitated cell apoptosis in vitro, and inhibited tumorigenicity of CRC cells in vivo. Mechanistically, RNA immunoprecipitation (RIP) and RNA pull-down experiment demonstrated that HULC could simultaneously interact with EZH2 to repress underlying targets NKD2 transcription. In addition, rescue experiments determined that HULC oncogenic function is partly dependent on repressing NKD2. Taken together, our findings expound how HULC over-expression endows an oncogenic function in CRC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=long%20noncoding%20RNA" title="long noncoding RNA">long noncoding RNA</a>, <a href="https://publications.waset.org/abstracts/search?q=HULC" title=" HULC"> HULC</a>, <a href="https://publications.waset.org/abstracts/search?q=NKD2" title=" NKD2"> NKD2</a>, <a href="https://publications.waset.org/abstracts/search?q=colorectal%20carcinoma" title=" colorectal carcinoma"> colorectal carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=proliferation" title=" proliferation"> proliferation</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title=" apoptosis"> apoptosis</a> </p> <a href="https://publications.waset.org/abstracts/54941/role-of-long-noncoding-rna-hulc-on-colorectal-carcinoma-progression-through-epigenetically-repressing-nkd2-expression" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54941.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">225</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4333</span> An in vitro Study on Synergetic Antifungal Activity of Garlic Extract with Honey and Lemon Juice against Candida sp.</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20Karpagam">P. Karpagam</a>, <a href="https://publications.waset.org/abstracts/search?q=Babu%20Joseph"> Babu Joseph</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Ashok%20Kumar"> P. Ashok Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The incidence of Candida infections is increasing worldwide. The serious nature of these infections is compounded by increasing levels of drug resistance. Pure cultures of the Candida sp. were obtained from clinical isolates and fresh garlic extracts were obtained by extraction techniques. The antifungal activity of garlic extract was investigated in an in vitro system. The extract (100%, 75% and 50%) showed significant antifungal activity against Candida, whereas, low concentration (25%) of the extract showed less antifungal activity against the test organism. Antifungal activities of honey and lemon juice were tested against the Candida; however, the growth was not inhibited by these extracts. On the other hand honey and lemon when combined with garlic exhibited a good antifungal activity. The study thus confirms the antifungal properties of garlic extract along with additives like honey and lemon have significant antifungal activity against isolates of Candida species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Candida" title="Candida">Candida</a>, <a href="https://publications.waset.org/abstracts/search?q=garlic%20extract" title=" garlic extract"> garlic extract</a>, <a href="https://publications.waset.org/abstracts/search?q=lemon" title=" lemon"> lemon</a>, <a href="https://publications.waset.org/abstracts/search?q=synergitic%20antifungal%20activity" title=" synergitic antifungal activity"> synergitic antifungal activity</a> </p> <a href="https://publications.waset.org/abstracts/75056/an-in-vitro-study-on-synergetic-antifungal-activity-of-garlic-extract-with-honey-and-lemon-juice-against-candida-sp" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75056.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">250</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4332</span> Effect of Ginger Diets on in vitro Fermentation Characteristics, Enteric Methane Production and Performance of West African Dwarf Sheep</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dupe%20Olufunke%20Ogunbosoye">Dupe Olufunke Ogunbosoye</a>, <a href="https://publications.waset.org/abstracts/search?q=Thaofik%20Badmos%20Mustapha"> Thaofik Badmos Mustapha</a>, <a href="https://publications.waset.org/abstracts/search?q=Lanre%20Shaffihy%20Adeaga"> Lanre Shaffihy Adeaga</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20O.%20Imam"> R. O. Imam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Efforts have been made to reduce ruminants' methane emissions while improving animal productivity. Hence, an experiment was conducted to investigate the in vitro fermentation pattern, methane production, and performance of West African dwarf (WAD) rams-fed diets at graded levels of ginger. Sixteen (16) rams were randomly allocated into four dietary treatments with four animals per treatment in a completely randomized design for 84 days. Ginger powder was added at 0.00%, 0.25%, 0.50% and 0.75% as T1, T2, T3 and T4 respectively. The results indicated that at the 24-hour diet incubation, gas production, methane, metabolizable energy (ME), organic matter digestibility (OMD), and short-chain fatty acids (SCFA) concentrations decreased with the increasing level of ginger. Conversely, the sheep-fed T4 recorded the highest daily weight gain (47.61g/day), while the least daily weight gain (17.86g/day) was recorded in ram-fed T1. The daily weight gain of the rams fed T3 and T4 was similar but significantly different from the daily weight gain in T1 (17.86g/day) and T2 (29.76g/day). Daily feed intake was not significantly different across the treatments. T4 recorded the best response regarding feed conversion ratio (18.59) compared with other treatments. Based on the results obtained, rams fed T4 perform best in terms of growth and methane production. It is therefore concluded that the addition of ginger powder into the diet of sheep up to 0.75% enhances the growth rate of WAD sheep and reduces enteric methane production to create a smart nutrition system in ruminant animal production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=enteric%20methane" title="enteric methane">enteric methane</a>, <a href="https://publications.waset.org/abstracts/search?q=growth" title=" growth"> growth</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro" title=" in vitro"> in vitro</a>, <a href="https://publications.waset.org/abstracts/search?q=sheep" title=" sheep"> sheep</a>, <a href="https://publications.waset.org/abstracts/search?q=nutrition%20system" title=" nutrition system"> nutrition system</a> </p> <a href="https://publications.waset.org/abstracts/171151/effect-of-ginger-diets-on-in-vitro-fermentation-characteristics-enteric-methane-production-and-performance-of-west-african-dwarf-sheep" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171151.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">78</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4331</span> Responses of Trifolium pratense to Lead Accumulation Under In-Vitro Culture Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arash%20Khorasani%20Esmaeili">Arash Khorasani Esmaeili</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosna%20Mat%20Taha"> Rosna Mat Taha</a>, <a href="https://publications.waset.org/abstracts/search?q=Sadegh%20Mohajer"> Sadegh Mohajer</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Seeds of Trifolium pratense (Red clover) were exposed in vitro for 6 weeks to six levels of lead (Pb) concentrations (0, 50, 100, 150, 200, 250 µM) to analyze the effects on growth, total chlorophyll and total protein contents of grown plants against the lead accumulation. The growth of plants was negatively affected by various levels of lead treatment. The fresh and dry weights, as well as lengths of shoots and roots of grown plants under various lead treatments, were found significantly lower in comparison with the control plants. Total chlorophyll and total soluble protein contents of grown plants under lower concentrations of lead treatment did not show significant differences when compared with the control plants, although they were affected significantly in higher levels of lead accumulation (150-250 µM). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=trifolium%20pratense" title="trifolium pratense">trifolium pratense</a>, <a href="https://publications.waset.org/abstracts/search?q=lead%20accumulation" title=" lead accumulation"> lead accumulation</a>, <a href="https://publications.waset.org/abstracts/search?q=chlorophyll%20content" title=" chlorophyll content"> chlorophyll content</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%20content" title=" protein content"> protein content</a> </p> <a href="https://publications.waset.org/abstracts/32059/responses-of-trifolium-pratense-to-lead-accumulation-under-in-vitro-culture-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32059.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">437</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4330</span> Cytokine Profiling in Cultured Endometrial Cells after Hormonal Treatment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mark%20Gavriel">Mark Gavriel</a>, <a href="https://publications.waset.org/abstracts/search?q=Ariel%20J.%20Jaffa"> Ariel J. Jaffa</a>, <a href="https://publications.waset.org/abstracts/search?q=Dan%20Grisaru"> Dan Grisaru</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20Elad"> David Elad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The human endometrium-myometrium interface (EMI) is the uterine inner barrier without a separatig layer. It is composed of endometrial epithelial cells (EEC) and endometrial stromal cells (ESC) in the endometrium and myometrial smooth muscle cells (MSMC) in the myometrium. The EMI undergoes structural remodeling during the menstruation cycle which are essential for human reproduction. Recently, we co-cultured a layer-by-layer in vitro model of EEC, ESC and MSMC on a synthetic membrane for mechanobiology experiments. We also treated the model with progesterone and β-estradiol in order to mimic the in vivo receptive uterus In the present study we analyzed the cytokines profile in a single layer of EEC the hormonal treated in vitro model of the EMI. The methodologies of this research include simple tissue-engineering . First, we cultured commercial EEC (RL95-2, ATCC® CRL-1671™) in 24-wellplate. Then, we applied an hormonal stimuli protocol with 17-β-estradiol and progesterone in time dependent concentration according to the human physiology that mimics the menstrual cycle. We collected cell supernatant samples of control, pre-ovulation, ovulation and post-ovulaton periods for analysis of the secreted proteins and cytokines. The cytokine profiling was performed using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, Inc., USA) that can detect105 human soluble cytokines. The relative quantification of all the cytokines will be analyzed using xMAP – LUMINEX. We conducted a fishing expedition with the 4 membranes Proteome Profiler. We processed the images, quantified the spots intensity and normalized these values by the negative control and reference spots at the membrane. Analyses of the relative quantities that reflected change higher than 5% of the control points of the kit revealed the The results clearly showed that there are significant changes in the cytokine level for inflammation and angiogenesis pathways. Analysis of tissue-engineered models of the uterine wall will enable deeper investigation of molecular and biomechanical aspects of early reproductive stages (e.g. the window of implantation) or developments of pathologies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=tissue-engineering" title="tissue-engineering">tissue-engineering</a>, <a href="https://publications.waset.org/abstracts/search?q=hormonal%20stimuli" title=" hormonal stimuli"> hormonal stimuli</a>, <a href="https://publications.waset.org/abstracts/search?q=reproduction" title=" reproduction"> reproduction</a>, <a href="https://publications.waset.org/abstracts/search?q=multi-layer%20uterine%20model" title=" multi-layer uterine model"> multi-layer uterine model</a>, <a href="https://publications.waset.org/abstracts/search?q=progesterone" title=" progesterone"> progesterone</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B2-estradiol" title=" β-estradiol"> β-estradiol</a>, <a href="https://publications.waset.org/abstracts/search?q=receptive%20uterine%20model" title=" receptive uterine model"> receptive uterine model</a>, <a href="https://publications.waset.org/abstracts/search?q=fertility" title=" fertility"> fertility</a> </p> <a href="https://publications.waset.org/abstracts/149276/cytokine-profiling-in-cultured-endometrial-cells-after-hormonal-treatment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/149276.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">132</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4329</span> Antioxidant Activity and Hepatoprotective Potential of Genista quadriflora Munby against Paracetamol-Induced Liver Injury</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nacera%20Baali">Nacera Baali</a>, <a href="https://publications.waset.org/abstracts/search?q=Zahia%20Belloum"> Zahia Belloum</a>, <a href="https://publications.waset.org/abstracts/search?q=Souad%20Ameddah"> Souad Ameddah</a>, <a href="https://publications.waset.org/abstracts/search?q=Fadila%20Benayache"> Fadila Benayache</a>, <a href="https://publications.waset.org/abstracts/search?q=Samir%20Benayache"> Samir Benayache</a>, <a href="https://publications.waset.org/abstracts/search?q=Chantal%20Wrutniak-Cabello"> Chantal Wrutniak-Cabello</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Allurement of herbs as health beneficial foods and as a source material for the development of new drugs, has led to greater furtherance in the study of herbal medicines during recent years. In the present study, in vitro antioxidant, free radical scavenging capacity, and hepatoprotective activity of butanolic extract from Genista quadriflora Munby (G.quadriflora) were evaluated using established in vitro models such as DPPH radical and hydrogen peroxide radical scavenging activities and antilipidperoxidation ability. Interestingly, the extract showed considerable in vitro antioxidant and free radical scavenging activities in a dose-dependent manner when compared to the standard antioxidant which verified the presence of antioxidant compound in extract tested. The hepatoprotective potential of G.quadriflora extract was also evaluated in male Wistar rats against paracetamol (APAP) induced liver damage. Therapy of G.quadriflora showed the liver protective effect on biochemical and histopathological alterations. Moreover, histological studies also supported the biochemical finding, that is, the maximum improvement in the histoarchitecture of the liver. Results revealed that G.quadriflora extract could protect the liver against APAP-induced oxidative damage by possibly increasing the antioxidant protection mechanism in rats. These findings are of great importance in view of the availability of the plant and its observed possible diverse applications in medicine and nutrition. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=genista%20quadriflora%20munby" title="genista quadriflora munby">genista quadriflora munby</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=liver" title=" liver"> liver</a>, <a href="https://publications.waset.org/abstracts/search?q=paracetamol" title=" paracetamol"> paracetamol</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a> </p> <a href="https://publications.waset.org/abstracts/31214/antioxidant-activity-and-hepatoprotective-potential-of-genista-quadriflora-munby-against-paracetamol-induced-liver-injury" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31214.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">473</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4328</span> Investigating the Essentiality of Oxazolidinones in Resistance-Proof Drug Combinations in Mycobacterium tuberculosis Selected under in vitro Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gail%20Louw">Gail Louw</a>, <a href="https://publications.waset.org/abstracts/search?q=Helena%20Boshoff"> Helena Boshoff</a>, <a href="https://publications.waset.org/abstracts/search?q=Taeksun%20Song"> Taeksun Song</a>, <a href="https://publications.waset.org/abstracts/search?q=Clifton%20Barry"> Clifton Barry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Drug resistance in Mycobacterium tuberculosis is primarily attributed to mutations in target genes. These mutations incur a fitness cost and result in bacterial generations that are less fit, which subsequently acquire compensatory mutations to restore fitness. We hypothesize that mutations in specific drug target genes influence bacterial metabolism and cellular function, which affects its ability to develop subsequent resistance to additional agents. We aim to determine whether the sequential acquisition of drug resistance and specific mutations in a well-defined clinical M. tuberculosis strain promotes or limits the development of additional resistance. In vitro mutants resistant to pretomanid, linezolid, moxifloxacin, rifampicin and kanamycin were generated from a pan-susceptible clinical strain from the Beijing lineage. The resistant phenotypes to the anti-TB agents were confirmed by the broth microdilution assay and genetic mutations were identified by targeted gene sequencing. Growth of mono-resistant mutants was done in enriched medium for 14 days to assess in vitro fitness. Double resistant mutants were generated against anti-TB drug combinations at concentrations 5x and 10x the minimum inhibitory concentration. Subsequently, mutation frequencies for these anti-TB drugs in the different mono-resistant backgrounds were determined. The initial level of resistance and the mutation frequencies observed for the mono-resistant mutants were comparable to those previously reported. Targeted gene sequencing revealed the presence of known and clinically relevant mutations in the mutants resistant to linezolid, rifampicin, kanamycin and moxifloxacin. Significant growth defects were observed for mutants grown under in vitro conditions compared to the sensitive progenitor. Mutation frequencies determination in the mono-resistant mutants revealed a significant increase in mutation frequency against rifampicin and kanamycin, but a significant decrease in mutation frequency against linezolid and sutezolid. This suggests that these mono-resistant mutants are more prone to develop resistance to rifampicin and kanamycin, but less prone to develop resistance against linezolid and sutezolid. Even though kanamycin and linezolid both inhibit protein synthesis, these compounds target different subunits of the ribosome, thereby leading to different outcomes in terms of fitness in the mutants with impaired cellular function. These observations showed that oxazolidinone treatment is instrumental in limiting the development of multi-drug resistance in M. tuberculosis in vitro. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=oxazolidinones" title="oxazolidinones">oxazolidinones</a>, <a href="https://publications.waset.org/abstracts/search?q=mutations" title=" mutations"> mutations</a>, <a href="https://publications.waset.org/abstracts/search?q=resistance" title=" resistance"> resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=tuberculosis" title=" tuberculosis"> tuberculosis</a> </p> <a href="https://publications.waset.org/abstracts/86067/investigating-the-essentiality-of-oxazolidinones-in-resistance-proof-drug-combinations-in-mycobacterium-tuberculosis-selected-under-in-vitro-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86067.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">162</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4327</span> A Sub-Conjunctiva Injection of Rosiglitazone for Anti-Fibrosis Treatment after Glaucoma Filtration Surgery</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yang%20Zhao">Yang Zhao</a>, <a href="https://publications.waset.org/abstracts/search?q=Feng%20Zhang"> Feng Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xuanchu%20Duan"> Xuanchu Duan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Trans-differentiation of human Tenon fibroblasts (HTFs) to myo-fibroblasts and fibrosis of episcleral tissue are the most common reasons for the failure of glaucoma filtration surgery, with limited treatment options like antimetabolites which always have side-effects such as leakage of filter bulb, infection, hypotony, and endophthalmitis. Rosiglitazone, a specific thiazolidinedione is a synthetic high-affinity ligand for PPAR-r, which has been used in the treatment of type2 diabetes, and found to have pleiotropic functions against inflammatory response, cell proliferation and tissue fibrosis and to benefit to a variety of diseases in animal myocardium models, steatohepatitis models, etc. Here, in vitro we cultured primary HTFs and stimulated with TGF- β to induced myofibrogenic, then treated cells with Rosiglitazone to assess for fibrogenic response. In vivo, we used rabbit glaucoma model to establish the formation of post- trabeculectomy scarring. Then we administered subconjunctival injection with Rosiglitazone beside the ﬁltering bleb, later protein, mRNA and immunofluorescence of fibrogenic markers are checked, and filtering bleb condition was measured. In vitro, we found Rosiglitazone could suppressed proliferation and migration of fibroblasts through macroautophagy via TGF- β /Smad signaling pathway. In vivo, on postoperative day 28, the mean number of ﬁbroblasts in Rosiglitazone injection group was signiﬁcantly the lowest and had the least collagen content and connective tissue growth factor. Rosiglitazone effectively controlled human and rabbit fibroblasts in vivo and in vitro. Its subconjunctiiva application may represent an effective, new avenue for the prevention of scarring after glaucoma surgery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fibrosis" title="fibrosis">fibrosis</a>, <a href="https://publications.waset.org/abstracts/search?q=glaucoma" title=" glaucoma"> glaucoma</a>, <a href="https://publications.waset.org/abstracts/search?q=macroautophagy" title=" macroautophagy"> macroautophagy</a>, <a href="https://publications.waset.org/abstracts/search?q=rosiglitazone" title=" rosiglitazone"> rosiglitazone</a> </p> <a href="https://publications.waset.org/abstracts/73539/a-sub-conjunctiva-injection-of-rosiglitazone-for-anti-fibrosis-treatment-after-glaucoma-filtration-surgery" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/73539.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">274</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4326</span> Heterologous Expression of a Clostridium thermocellum Proteins and Assembly of Cellulosomes 'in vitro' for Biotechnology Applications</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jessica%20Pinheiro%20Silva">Jessica Pinheiro Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Brenda%20Rabello%20De%20Camargo"> Brenda Rabello De Camargo</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Gusmao%20De%20Morais"> Daniel Gusmao De Morais</a>, <a href="https://publications.waset.org/abstracts/search?q=Eliane%20%20Ferreira%20Noronha"> Eliane Ferreira Noronha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The utilization of lignocellulosic biomass as source of polysaccharides for industrial applications requires an arsenal of enzymes with different mode of action able to hydrolyze its complex and recalcitrant structure. Clostridium thermocellum is gram-positive, thermophilic bacterium producing lignocellulosic hydrolyzing enzymes in the form of multi-enzyme complex, termed celulossomes. This complex has several hydrolytic enzymes attached to a large and enzymically inactive protein known as Cellulosome-integrating protein (CipA), which serves as a scaffolding protein for the complex produced. This attachment occurs through specific interactions between cohesin modules of CipA and dockerin modules in enzymes. The present work aims to construct celulosomes in vitro with the structural protein CipA, a xylanase called Xyn10D and a cellulose called CelJ from C.thermocellum. A mini-scafoldin was constructed from modules derived from CipA containing two cohesion modules. This was cloned and expressed in Escherichia coli. The other two genes were cloned under the control of the alcohol oxidase 1 promoter (AOX1) in the vector pPIC9 and integrated into the genome of the methylotrophic yeast Pichia pastoris GS115. Purification of each protein is being carried out. Further studies regarding enzymatic activity of the cellulosome is going to be evaluated. The cellulosome built in vitro and composed of mini-CipA, CelJ and Xyn10D, can be very interesting for application in industrial processes involving the degradation of plant biomass. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cellulosome" title="cellulosome">cellulosome</a>, <a href="https://publications.waset.org/abstracts/search?q=CipA" title=" CipA"> CipA</a>, <a href="https://publications.waset.org/abstracts/search?q=Clostridium%20thermocellum" title=" Clostridium thermocellum"> Clostridium thermocellum</a>, <a href="https://publications.waset.org/abstracts/search?q=cohesin" title=" cohesin"> cohesin</a>, <a href="https://publications.waset.org/abstracts/search?q=dockerin" title=" dockerin"> dockerin</a>, <a href="https://publications.waset.org/abstracts/search?q=yeast" title=" yeast"> yeast</a> </p> <a href="https://publications.waset.org/abstracts/79260/heterologous-expression-of-a-clostridium-thermocellum-proteins-and-assembly-of-cellulosomes-in-vitro-for-biotechnology-applications" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/79260.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">233</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4325</span> In Vitro Hepatoprotective and Anti-Hepatitis B Activitis of Cyperus rotundus Rhizome Fractions </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20K.%20Parvez">Mohammad K. Parvez</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20H.%20Arbab"> Ahmed H. Arbab</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20S.%20Al-Dosari"> Mohammed S. Al-Dosari </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cyperus rotendus rhizomes are used as traditional medicine, including Ayurveda in chronic liver diseases and hepatitis B. We investigated the in vitro hepatoprotective and anti-hepatitis B virus (HBV) potential of Cyperus rotundus rhizome organic and aqueous fractions. Of these, the n-butanol and aqueous fractions showed the most promising, dose-dependent hepatoprotection in DCFH-injured HepG2 cells at 48 h. DCFH-toxicated cells were recovered to about 88% and 96%, upon treatment with n-butanol and aqueous fractions (200 g/ml), respectively compared to DCFH-only treated cells. Further, C. rotundus fractions were tested for anti-HBV activities by measuring the expression levels of viral antigens (HBsAg and HBeAg) in the HepG2.2.15 culture supernatants. At 48 h post-treatment, the ethyl acetate, n-butanol and aqueous fractions showed dose-dependent inhibition wherein at a higher dose (100 g/ml), HBsAg production was reduced to 60.27%, 46.87 and 42.76%, respectively. In a time-course study, HBsAg production was inhibited up to 50% and 40% by ethyl acetate and n-butanol fractions (100 g/ml), respectively on day 5. Three three active fractions were further subjected to time-dependent inhibition of HBeAg expression, an indirect measure of HBV active DNA replication. At day 5 post-treatment, ethyl acetate and n-butanol fractions downregulated HBV replication by 44.14% and 24.70%, respectively. In conclusion, our results showed very promising hepatoprotective and anti-HBV potential of C. rotendus tubers fractions in vitro. Our data could, therefore, provide the basis for the claimed traditional use of C. rotendus for jaundice and hepatitis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anti-hepatitis%20B" title="anti-hepatitis B">anti-hepatitis B</a>, <a href="https://publications.waset.org/abstracts/search?q=cyperus%20rotundus" title=" cyperus rotundus"> cyperus rotundus</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatitis%20B%20virus" title=" hepatitis B virus"> hepatitis B virus</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatoprotection" title=" hepatoprotection"> hepatoprotection</a> </p> <a href="https://publications.waset.org/abstracts/46692/in-vitro-hepatoprotective-and-anti-hepatitis-b-activitis-of-cyperus-rotundus-rhizome-fractions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46692.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">235</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4324</span> Sensitivity of Acanthamoeba castellanii-Grown Francisella to Three Different Disinfectants</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Knezevic">M. Knezevic</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Marecic"> V. Marecic</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Ozanic"> M. Ozanic</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Kelava"> I. Kelava</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Mihelcic"> M. Mihelcic</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Santic"> M. Santic</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Francisella tularensis is a highly infectious, gram-negative intracellular bacterium and the causative agent of tularemia. The bacterium has been isolated from more than 250 wild species, including protozoa cells. Since Francisella is very virulent and persists in the environment for years, the aim of this study was to investigate whether Acanthamoeba castellanii-grown F. novicida exhibits an alteration in the resistance to disinfectants. It has been shown by other intracellular pathogens, including Legionella pneumophila that bacteria grown in amoeba exhibit more resistance to disinfectants. However, there is no data showing Francisella viability behaviour after intracellular life cycle in A. castellani. In this study, the bacterial suspensions of A. castellanii-grown or in vitro-grown Francisella were treated with three different disinfectants, and the bacterial viability after disinfection treatment was determined by a colony-forming unit (CFU) counting method, transmission electron microscopy (TEM), fluorescence microscopy as well as the leakage of intracellular fluid. Our results have shown that didecyldimethylammonium chloride (DDAC) combined with isopropyl alcohol was the most effective in bacterial killing; all in vitro-grown and A. castellanii-grown F. novicida were killed after only 10s. Surprisingly, in comparison to in vitro-grown bacteria, A. castellanii-grown F. novicida was more sensitive to decontamination by the benzalkonium chloride combined with DDAC and formic acid and the polyhexamethylene biguanide (PHMB). We can conclude that the tested disinfectants exhibit antimicrobial activity by causing a loss of structural organization and integrity of the Francisella cell wall and membrane and the subsequent leakage of the intracellular contents. Finally, the results of this study clearly demonstrate that Francisella grown in A. castellanii had become more susceptible to many disinfectants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acanthamoeba" title="Acanthamoeba">Acanthamoeba</a>, <a href="https://publications.waset.org/abstracts/search?q=disinfectant" title=" disinfectant"> disinfectant</a>, <a href="https://publications.waset.org/abstracts/search?q=Francisella" title=" Francisella"> Francisella</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity" title=" sensitivity"> sensitivity</a> </p> <a href="https://publications.waset.org/abstracts/132477/sensitivity-of-acanthamoeba-castellanii-grown-francisella-to-three-different-disinfectants" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/132477.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">101</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4323</span> Triggering Apoptosis to Uproot Breast Cancer: HPLC-MS/MS Profiling, in-vitro and in-silico Fascinating Results of Polyphenolics in Pomegranate Rind Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alaa%20M.%20Badr%20Eldin">Alaa M. Badr Eldin</a>, <a href="https://publications.waset.org/abstracts/search?q=Mayar%20M.%20Shahen"> Mayar M. Shahen</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20S.%20Sedeek"> Mohammed S. Sedeek</a>, <a href="https://publications.waset.org/abstracts/search?q=Marwa%20I.%20Ezzat"> Marwa I. Ezzat</a>, <a href="https://publications.waset.org/abstracts/search?q=Sawsan%20M.%20ElSonbaty"> Sawsan M. ElSonbaty</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammed%20A.%20Saad"> Muhammed A. Saad</a>, <a href="https://publications.waset.org/abstracts/search?q=Manal%20S.%20Afifi"> Manal S. Afifi</a>, <a href="https://publications.waset.org/abstracts/search?q=Omar%20M.%20Sabry"> Omar M. Sabry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Using HPLC-MS/MS technique, 133 polyphenolic compounds were identified in the methanol extract of pomegranate rind (Punica granatum L.). In-vitro cytotoxic activity against breast cancer cell line MCF-7 was investigated, with an IC50 of 54 ug/ml. In-silico molecular docking using ellagic acid, gallagic acid, and Punicalagin as model compounds identified in pomegranate rind extract confirmed the intriguing anti-estrogenic action of the key polyphenolic components in pomegranate rind extract. Surprisingly, taxol showed low activity compared to pomegranate compounds as ERα antagonist and ERβ agonist. Pomegranate rind extract enhanced apoptosis of breast cancer cells through upregulation of the caspase-3 expression and downregulation of NF-κB transcription factor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=HPLC-MS%2FMS" title="HPLC-MS/MS">HPLC-MS/MS</a>, <a href="https://publications.waset.org/abstracts/search?q=pomegranate%20rind" title=" pomegranate rind"> pomegranate rind</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=MCF-7" title=" MCF-7"> MCF-7</a>, <a href="https://publications.waset.org/abstracts/search?q=ER" title=" ER"> ER</a>, <a href="https://publications.waset.org/abstracts/search?q=caspase-3" title=" caspase-3"> caspase-3</a>, <a href="https://publications.waset.org/abstracts/search?q=NF-kB" title=" NF-kB"> NF-kB</a> </p> <a href="https://publications.waset.org/abstracts/163413/triggering-apoptosis-to-uproot-breast-cancer-hplc-msms-profiling-in-vitro-and-in-silico-fascinating-results-of-polyphenolics-in-pomegranate-rind-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163413.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">116</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4322</span> Superiority of Bone Marrow Derived-Osteoblastic Cells (ALLOB®) over Bone Marrow Derived-Mesenchymal Stem Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sandra%20Pietri">Sandra Pietri</a>, <a href="https://publications.waset.org/abstracts/search?q=Helene%20Dubout"> Helene Dubout</a>, <a href="https://publications.waset.org/abstracts/search?q=Sabrina%20Ena"> Sabrina Ena</a>, <a href="https://publications.waset.org/abstracts/search?q=Candice%20Hoste"> Candice Hoste</a>, <a href="https://publications.waset.org/abstracts/search?q=Enrico%20Bastianelli"> Enrico Bastianelli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bone Therapeutics is a bone cell therapy company addressing high unmet medical needs in the field of bone fracture repair, more specifically in non-union and delayed-union fractures where the bone repair process is impaired. The company has developed a unique allogeneic osteoblastic cell product (ALLOB®) derived from bone marrow which is currently tested in humans in the indication of delayed-union fractures. The purpose of our study was to directly compare ALLOB® vs. non-differentiated mesenchymal stem cells (MSC) for their in vitro osteogenic characteristics and their in vivo osteogenic potential in order to determine which cellular type would be the most adapted for bone fracture repair. Methods: Healthy volunteers’ bone marrow aspirates (n=6) were expended (i) into BM-MSCs using a complete MSC culture medium or (ii) into ALLOB® cells according to its manufacturing process. Cells were characterized in vitro by morphology, immunophenotype, gene expression and differentiation potential. Additionally, their osteogenic potential was assessed in vivo in the subperiosteal calvaria bone formation model in nude mice. Results: The in vitro side-by-side comparison studies showed that although ALLOB® and BM-MSC shared some common general characteristics such as the 3 minimal MSC criteria, ALLOB® expressed significantly higher levels of chondro/osteoblastic genes such as BMP2 (fold change (FC) > 100), ALPL (FC > 12), CBFA1 (FC > 3) and differentiated significantly earlier than BM-MSC toward the osteogenic lineage. Moreover the bone formation model in nude mice demonstrated that used at the same cellular concentration, ALLOB® was able to induce significantly more (160% vs.107% for control animals) bone formation than BM-MSC (118% vs. 107 % for control animals) two weeks after administration. Conclusion: Our side-by-side comparison studies demonstrated that in vitro and in vivo, ALLOB® displays superior osteogenic capacity to BM-MScs and is therefore a better candidate for the treatment of bone fractures. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gene%20expression" title="gene expression">gene expression</a>, <a href="https://publications.waset.org/abstracts/search?q=histomorphometry" title=" histomorphometry"> histomorphometry</a>, <a href="https://publications.waset.org/abstracts/search?q=mesenchymal%20stem%20cells" title=" mesenchymal stem cells"> mesenchymal stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=osteogenic%20differentiation%20potential" title=" osteogenic differentiation potential"> osteogenic differentiation potential</a>, <a href="https://publications.waset.org/abstracts/search?q=preclinical" title=" preclinical"> preclinical</a> </p> <a href="https://publications.waset.org/abstracts/44433/superiority-of-bone-marrow-derived-osteoblastic-cells-allob-over-bone-marrow-derived-mesenchymal-stem-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44433.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">330</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4321</span> Collagen Silver Lipid Nanoparticles as Matrix and Fillers for Cosmeceuticals: An In-Vitro and In-Vivo Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kumari%20Kajal">Kumari Kajal</a>, <a href="https://publications.waset.org/abstracts/search?q=Muthu%20Kumar%20Sampath"> Muthu Kumar Sampath</a>, <a href="https://publications.waset.org/abstracts/search?q=Hare%20Ram%20Singh"> Hare Ram Singh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this context, the formulation and characterization of collagen silver lipid nanoparticles (CSLNs) were studied for their capacity to serve as fillers/matrix materials used in cosmeceutical applications. The CSLNs were prepared following a series of studies, such as X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM) coupled with energy-dispersive X-ray spectroscopy (EDS), Fourier-transform infrared spectroscopy FT-IR; thermogravimetric analysis (TGA); and differential scanning calorimetry (DSC). The studies confirmed the structural integrity of nanoparticles, their cargo and thermal stability. The biological functionality of CSLNs was studied by carrying out in vitro & in vivo studies. The antibacterial effect, hemocompatibility and anti-inflammatory characteristics of these fibers were systematically investigated. The toxicological assays included oral toxicity in mice and aquatic life tests with the fish Danio rerio model. The morphology of the nanoparticles was confirmed using high-resolution transmission electron microscopy (HR-TEM). The report found that CSLNs had strong antimicrobial effects, unmatched hemocompatibility, and low or absent inflammatory reactions, which makes them perfect candidates for cosmeceutical applications. The toxicological evaluations evinced a good safety record without any significant adverse effects in both murine and Danio rerio models. This research reveals the efficient way of CSLNs to the efficacy and safety of dermaceuticals. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=collagen%20silver%20lipid%20nanoparticles%20%28CSLNs%29" title="collagen silver lipid nanoparticles (CSLNs)">collagen silver lipid nanoparticles (CSLNs)</a>, <a href="https://publications.waset.org/abstracts/search?q=cosmeceuticals" title=" cosmeceuticals"> cosmeceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activity" title=" antimicrobial activity"> antimicrobial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=hemocompatibility" title=" hemocompatibility"> hemocompatibility</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20assessment" title=" in vitro assessment"> in vitro assessment</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo%20assessment." title=" in vivo assessment."> in vivo assessment.</a> </p> <a href="https://publications.waset.org/abstracts/193215/collagen-silver-lipid-nanoparticles-as-matrix-and-fillers-for-cosmeceuticals-an-in-vitro-and-in-vivo-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193215.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">15</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4320</span> Biological Activity of Hibiscus sabdariffa Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chanasit%20Chaocharoenphat">Chanasit Chaocharoenphat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hibiscus sabdariffa is a herbal plant that is commonly used for home remedies in Thailand. This study aims to determine the antioxidant activity of polyphenols, as oxidative stress plays a vital role in the development of cancer, and H. sabdariffa was used in this study. The total flavonoids content was determined using the aluminium chloride colourimetric method and expressed as quercetin equivalents (QE)/g and the antioxidant capacity of the flavonoids using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity assays. The IC50 values of H. sabdariffa extract were 167.14 μg/mL ± 0.843 and 77.59 μg/mL ± 0.798, respectively. In the DPPH assay, vitamin C was used as a positive control, whereas Trolox was used as a positive control in the ABTS assay. To summarise, H. sabdariffa extract contains a high concentration of total flavonoids and exhibits potent antioxidant activity. However, additional antioxidant activity assays such as superoxide dismutase (SOD), reactive oxygen species (ROS), and reactive nitrogen species (RNS) scavenging assays and in vitro antioxidant experiments should be carried out to investigate the molecular mechanism of the compound. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ABTS%20assay" title="ABTS assay">ABTS assay</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=Gracilaria%20fisheri" title=" Gracilaria fisheri"> Gracilaria fisheri</a>, <a href="https://publications.waset.org/abstracts/search?q=DPPH%20assays" title=" DPPH assays"> DPPH assays</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20flavonoid%20content" title=" total flavonoid content"> total flavonoid content</a> </p> <a href="https://publications.waset.org/abstracts/140790/biological-activity-of-hibiscus-sabdariffa-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/140790.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">242</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4319</span> Preparation and In vitro Characterization of Nanoparticle Hydrogel for Wound Healing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rajni%20Kant%20Panik">Rajni Kant Panik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of the present study was to develop and evaluate mupirocin loaded nanoparticle incorporated into hydrogel as an infected wound healer. Incorporated Nanoparticle in hydrogel provides a barrier that effectively prevents the contamination of the wound and further progression of infection to deeper tissues. Hydrogel creates moist healing environment on wound space with good fluid absorbance. Nanoparticles were prepared by double emulsion solvent evaporation method using different ratios of PLGA polymer and the hydrogels was developed using sodium alginate and gelatin. Further prepared nanoparticles were then incorporated into the hydrogels. The formulations were characterized by FT-IR and DSC for drug and polymer compatibility and surface morphology was studied by TEM. Nanoparticle hydrogel were evaluated for their size, shape, encapsulation efficiency and for in vitro studies. The FT-IR and DSC confirmed the absence of any drug polymer interaction. The average size of Nanoparticle was found to be in range of 208.21-412.33 nm and shape was found to be spherical. The maximum encapsulation efficiency was found to be 69.03%. The in vitro release profile of Nanoparticle incorporated hydrogel formulation was found to give sustained release of drug. Antimicrobial activity testing confirmed that encapsulated drug preserve its effectiveness. The stability study confirmed that the formulation prepared were stable. Present study complements our finding that mupirocin loaded Nanoparticle incorporated into hydrogel has the potential to be an effective and safe novel addition for the release of mupirocin in sustained manner, which may be a better option for the management of wound. These finding also supports the progression of antibiotic via hydrogel delivery system is a novel topical dosage form for the management of wound. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hydrogel" title="hydrogel">hydrogel</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticle" title=" nanoparticle"> nanoparticle</a>, <a href="https://publications.waset.org/abstracts/search?q=PLGA" title=" PLGA"> PLGA</a>, <a href="https://publications.waset.org/abstracts/search?q=wound%20healing" title=" wound healing"> wound healing</a> </p> <a href="https://publications.waset.org/abstracts/47734/preparation-and-in-vitro-characterization-of-nanoparticle-hydrogel-for-wound-healing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47734.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">311</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4318</span> Multitasking Incentives and Employee Performance: Evidence from Call Center Field Experiments and Laboratory Experiments</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sung%20Ham">Sung Ham</a>, <a href="https://publications.waset.org/abstracts/search?q=Chanho%20Song"> Chanho Song</a>, <a href="https://publications.waset.org/abstracts/search?q=Jiabin%20Wu"> Jiabin Wu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Employees are commonly incentivized on both quantity and quality performance and much of the extant literature focuses on demonstrating that multitasking incentives lead to tradeoffs. Alternatively, we consider potential solutions to the tradeoff problem from both a theoretical and an experimental perspective. Across two field experiments from a call center, we find that tradeoffs can be mitigated when incentives are jointly enhanced across tasks, where previous research has suggested that incentives be reduced instead of enhanced. In addition, we also propose and test, in a laboratory setting, the implications of revising the metric used to assess quality. Our results indicate that metrics can be adjusted to align quality and quantity more efficiently. Thus, this alignment has the potential to thwart the classic tradeoff problem. Finally, we validate our findings with an economic experiment that verifies that effort is largely consistent with our theoretical predictions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=incentives" title="incentives">incentives</a>, <a href="https://publications.waset.org/abstracts/search?q=multitasking" title=" multitasking"> multitasking</a>, <a href="https://publications.waset.org/abstracts/search?q=field%20experiment" title=" field experiment"> field experiment</a>, <a href="https://publications.waset.org/abstracts/search?q=experimental%20economics" title=" experimental economics"> experimental economics</a> </p> <a href="https://publications.waset.org/abstracts/88721/multitasking-incentives-and-employee-performance-evidence-from-call-center-field-experiments-and-laboratory-experiments" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88721.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4317</span> Recovery of Zn from Different Çinkur Leach Residues by Acidic Leaching</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mehmet%20Ali%20Top%C3%A7u">Mehmet Ali Topçu</a>, <a href="https://publications.waset.org/abstracts/search?q=Ayd%C4%B1n%20Ru%C5%9Fen"> Aydın Ruşen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Çinkur is the only plant in Turkey that produces zinc from primary ore containing zinc carbonate from its establishment until 1997. After this year, zinc concentrate coming from Iran was used in this plant. Therefore, there are two different leach residues namely Turkish leach residue (TLR) and Iranian leach residue (ILR), in Çinkur stock piles. This paper describes zinc recovery by sulphuric acid (H2SO4) treatment for each leach residue and includes comparison of blended of TLR and ILR. Before leach experiments; chemical, mineralogical and thermal analysis of three different leach residues was carried out by using atomic absorption spectrometry (AAS), X-Ray diffraction (XRD) and differential thermal analysis (DTA), respectively. Leaching experiments were conducted at optimum conditions; 100 oC, 150 g/L H2SO4 and 2 hours. In the experiments, stirring rate was kept constant at 600 r/min which ensures complete mixing in leaching solution. Results show that zinc recovery for Iranian LR was higher than Turkish LR due to having different chemical composition from each other. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hydrometallurgy" title="hydrometallurgy">hydrometallurgy</a>, <a href="https://publications.waset.org/abstracts/search?q=leaching" title=" leaching"> leaching</a>, <a href="https://publications.waset.org/abstracts/search?q=metal%20extraction" title=" metal extraction"> metal extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=metal%20recovery" title=" metal recovery "> metal recovery </a> </p> <a href="https://publications.waset.org/abstracts/37077/recovery-of-zn-from-different-cinkur-leach-residues-by-acidic-leaching" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37077.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">354</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4316</span> Modeling of Anisotropic Hardening Based on Crystal Plasticity Theory and Virtual Experiments</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bekim%20Berisha">Bekim Berisha</a>, <a href="https://publications.waset.org/abstracts/search?q=Sebastian%20Hirsiger"> Sebastian Hirsiger</a>, <a href="https://publications.waset.org/abstracts/search?q=Pavel%20Hora"> Pavel Hora</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Advanced material models involving several sets of model parameters require a big experimental effort. As models are getting more and more complex like e.g. the so called “Homogeneous Anisotropic Hardening - HAH” model for description of the yielding behavior in the 2D/3D stress space, the number and complexity of the required experiments are also increasing continuously. In the context of sheet metal forming, these requirements are even more pronounced, because of the anisotropic behavior or sheet materials. In addition, some of the experiments are very difficult to perform e.g. the plane stress biaxial compression test. Accordingly, tensile tests in at least three directions, biaxial tests and tension-compression or shear-reverse shear experiments are performed to determine the parameters of the macroscopic models. Therefore, determination of the macroscopic model parameters based on virtual experiments is a very promising strategy to overcome these difficulties. For this purpose, in the framework of multiscale material modeling, a dislocation density based crystal plasticity model in combination with a FFT-based spectral solver is applied to perform virtual experiments. Modeling of the plastic behavior of metals based on crystal plasticity theory is a well-established methodology. However, in general, the computation time is very high and therefore, the computations are restricted to simplified microstructures as well as simple polycrystal models. In this study, a dislocation density based crystal plasticity model – including an implementation of the backstress – is used in a spectral solver framework to generate virtual experiments for three deep drawing materials, DC05-steel, AA6111-T4 and AA4045 aluminum alloys. For this purpose, uniaxial as well as multiaxial loading cases, including various pre-strain histories, has been computed and validated with real experiments. These investigations showed that crystal plasticity modeling in the framework of Representative Volume Elements (RVEs) can be used to replace most of the expensive real experiments. Further, model parameters of advanced macroscopic models like the HAH model can be determined from virtual experiments, even for multiaxial deformation histories. It was also found that crystal plasticity modeling can be used to model anisotropic hardening more accurately by considering the backstress, similar to well-established macroscopic kinematic hardening models. It can be concluded that an efficient coupling of crystal plasticity models and the spectral solver leads to a significant reduction of the amount of real experiments needed to calibrate macroscopic models. This advantage leads also to a significant reduction of computational effort needed for the optimization of metal forming process. Further, due to the time efficient spectral solver used in the computation of the RVE models, detailed modeling of the microstructure are possible. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anisotropic%20hardening" title="anisotropic hardening">anisotropic hardening</a>, <a href="https://publications.waset.org/abstracts/search?q=crystal%20plasticity" title=" crystal plasticity"> crystal plasticity</a>, <a href="https://publications.waset.org/abstracts/search?q=micro%20structure" title=" micro structure"> micro structure</a>, <a href="https://publications.waset.org/abstracts/search?q=spectral%20solver" title=" spectral solver"> spectral solver</a> </p> <a href="https://publications.waset.org/abstracts/91272/modeling-of-anisotropic-hardening-based-on-crystal-plasticity-theory-and-virtual-experiments" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91272.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">315</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4315</span> Air Pollution: The Journey from Single Particle Characterization to in vitro Fate </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Potgieter-Vermaak">S. Potgieter-Vermaak</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Bain"> N. Bain</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Brown"> A. Brown</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Shaw"> K. Shaw</a> </p> <p class="card-text"><strong>Abstract:</strong></p> It is well-known from public news media that air pollution is a health hazard and is responsible for early deaths. The quantification of the relationship between air quality and health is a probing question not easily answered. It is known that airborne particulate matter (APM) <2.5µm deposits in the tracheal and alveoli zones and our research probes the possibility of quantifying pulmonary injury by linking reactive oxygen species (ROS) in these particles to DNA damage. Currently, APM mass concentration is linked to early deaths and limited studies probe the influence of other properties on human health. To predict the full extent and type of impact, particles need to be characterised for chemical composition and structure. APMs are routinely analysed for their bulk composition, but of late analysis on a micro level probing single particle character, using micro-analytical techniques, are considered. The latter, single particle analysis (SPA), permits one to obtain detailed information on chemical character from nano- to micron-sized particles. This paper aims to provide a snapshot of studies using data obtained from chemical characterisation and its link with in-vitro studies to inform on personal health risks. For this purpose, two studies will be compared, namely, the bioaccessibility of the inhalable fraction of urban road dust versus total suspended solids (TSP) collected in the same urban environment. The significant influence of metals such as Cu and Fe in TSP on DNA damage is illustrated. The speciation of Hg (determined by SPA) in different urban environments proved to dictate its bioaccessibility in artificial lung fluids rather than its concentration. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=air%20pollution" title="air pollution">air pollution</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20health" title=" human health"> human health</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro%20studies" title=" in-vitro studies"> in-vitro studies</a>, <a href="https://publications.waset.org/abstracts/search?q=particulate%20matter" title=" particulate matter"> particulate matter</a> </p> <a href="https://publications.waset.org/abstracts/68976/air-pollution-the-journey-from-single-particle-characterization-to-in-vitro-fate" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68976.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">225</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4314</span> Active Learning Based on Science Experiments to Improve Scientific Literacy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kunihiro%20Kamataki">Kunihiro Kamataki</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, active learning based on simple science experiments was developed in a university class of the freshman, in order to improve their scientific literacy. Through the active learning based on simple experiments of generation of cloud in a plastic bottle, students increased the interest in the global atmospheric problem and were able to discuss and find solutions about this problem positively from various viewpoints of the science technology, the politics, the economy, the diplomacy and the relations among nations. The results of their questionnaires and free descriptions of this class indicate that they improve the scientific literacy and motivations of other classroom lectures to acquire knowledge. It is thus suggested that the science experiment is strong tool to improve their intellectual curiosity rapidly and the connections that link the impression of science experiment and their interest of the social problem is very important to enhance their learning effect in this education. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=active%20learning" title="active learning">active learning</a>, <a href="https://publications.waset.org/abstracts/search?q=scientific%20literacy" title=" scientific literacy"> scientific literacy</a>, <a href="https://publications.waset.org/abstracts/search?q=simple%20scientific%20experiment" title=" simple scientific experiment"> simple scientific experiment</a>, <a href="https://publications.waset.org/abstracts/search?q=university%20education" title=" university education"> university education</a> </p> <a href="https://publications.waset.org/abstracts/47648/active-learning-based-on-science-experiments-to-improve-scientific-literacy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47648.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">261</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4313</span> Antidiabetic Effect of Methanolic Leaves Extract and Isolated Constituents from Saraca Asoca</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sunil%20Kumar">Sunil Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The present study was performed to investigate the antidiabetic effect of the constituents isolated from Sarca asoca by enzyme inhibitory activity. Methods: The dried leaves of Sarca asoca were defatted with petroleum ether and further the same amount plant materials were extracted with methanol. The dried methanol extract was subjected to fractionation and chromatographic separation, which led to the isolation of kaemferol, β-sitosterol and quercetin stigmasterol. Their structures were elucidated on the basis of spectroscopic studies as well as by comparison with the data available in the literature. The compounds were evaluated for in vitro enzyme inhibition effect. Results: The isolated compounds kaemferol, β-sitosterol and stigmasterol showed 45.32, 40.5 and 41.23% α-amylase inhibition respectively and 43.45, 39.29 and 32.43% α-glucosidase inhibition respectively at the conc. of 50 µg/kg. Conclusion: The compounds isolated from Sarca asoca showed in vitro and in vivo antidiabetic activity. So, Euphorbia hirta is a beneficial plant for management of diabetic disorders. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diabetes" title="diabetes">diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=quercetin" title=" quercetin"> quercetin</a>, <a href="https://publications.waset.org/abstracts/search?q=sitosterol" title=" sitosterol"> sitosterol</a>, <a href="https://publications.waset.org/abstracts/search?q=stigmasterol" title=" stigmasterol"> stigmasterol</a> </p> <a href="https://publications.waset.org/abstracts/30536/antidiabetic-effect-of-methanolic-leaves-extract-and-isolated-constituents-from-saraca-asoca" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30536.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">425</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4312</span> Innovative Preparation Techniques: Boosting Oral Bioavailability of Phenylbutyric Acid Through Choline Salt-Based API-Ionic Liquids and Therapeutic Deep Eutectic Systems</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lin%20Po-Hsi">Lin Po-Hsi</a>, <a href="https://publications.waset.org/abstracts/search?q=Sheu%20Ming-Thau"> Sheu Ming-Thau</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Urea cycle disorders (UCD) are rare genetic metabolic disorders that compromise the body's urea cycle. Sodium phenylbutyrate (SPB) is a medication commonly administered in tablet or powder form to lower ammonia levels. Nonetheless, its high sodium content poses risks to sodium-sensitive UCD patients. This necessitates the creation of an alternative drug formulation to mitigate sodium load and optimize drug delivery for UCD patients. This study focused on crafting a novel oral drug formulation for UCD, leveraging choline bicarbonate and phenylbutyric acid. The active pharmaceutical ingredient-ionic liquids (API-ILs) and therapeutic deep eutectic systems (THEDES) were formed by combining these with choline chloride. These systems display characteristics like maintaining a liquid state at room temperature and exhibiting enhanced solubility. This in turn amplifies drug dissolution rate, permeability, and ultimately oral bioavailability. Incorporating choline-based phenylbutyric acid as a substitute for traditional SPB can effectively curtail the sodium load in UCD patients. Our in vitro dissolution experiments revealed that the ILs and DESs, synthesized using choline bicarbonate and choline chloride with phenylbutyric acid, surpassed commercial tablets in dissolution speed. Pharmacokinetic evaluations in SD rats indicated a notable uptick in the oral bioavailability of phenylbutyric acid, underscoring the efficacy of choline salt ILs in augmenting its bioavailability. Additional in vitro intestinal permeability tests on SD rats authenticated that the ILs, formulated with choline bicarbonate and phenylbutyric acid, demonstrate superior permeability compared to their sodium and acid counterparts. To conclude, choline salt ILs developed from choline bicarbonate and phenylbutyric acid present a promising avenue for UCD treatment, with the added benefit of reduced sodium load. They also hold merit in formulation engineering. The sustained-release capabilities of DESs position them favorably for drug delivery, while the low toxicity and cost-effectiveness of choline chloride signal potential in formulation engineering. Overall, this drug formulation heralds a prospective therapeutic avenue for UCD patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phenylbutyric%20acid" title="phenylbutyric acid">phenylbutyric acid</a>, <a href="https://publications.waset.org/abstracts/search?q=sodium%20phenylbutyrate" title=" sodium phenylbutyrate"> sodium phenylbutyrate</a>, <a href="https://publications.waset.org/abstracts/search?q=choline%20salt" title=" choline salt"> choline salt</a>, <a href="https://publications.waset.org/abstracts/search?q=ionic%20liquids" title=" ionic liquids"> ionic liquids</a>, <a href="https://publications.waset.org/abstracts/search?q=deep%20eutectic%20systems" title=" deep eutectic systems"> deep eutectic systems</a>, <a href="https://publications.waset.org/abstracts/search?q=oral%20bioavailability" title=" oral bioavailability"> oral bioavailability</a> </p> <a href="https://publications.waset.org/abstracts/172061/innovative-preparation-techniques-boosting-oral-bioavailability-of-phenylbutyric-acid-through-choline-salt-based-api-ionic-liquids-and-therapeutic-deep-eutectic-systems" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/172061.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">116</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4311</span> Micropropagation of Rhododendron tomentosum (Ledum palustre): An Endangered Plant of Scientific Interest as the Example of Ex Situ Conservation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anna%20Jesionek">Anna Jesionek</a>, <a href="https://publications.waset.org/abstracts/search?q=Aleksandra%20Szreniawa-Sztajnert"> Aleksandra Szreniawa-Sztajnert</a>, <a href="https://publications.waset.org/abstracts/search?q=Zbigniew%20Jaremicz"> Zbigniew Jaremicz</a>, <a href="https://publications.waset.org/abstracts/search?q=Adam%20Kokotkiewicz"> Adam Kokotkiewicz</a>, <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Filipowicz"> Natalia Filipowicz</a>, <a href="https://publications.waset.org/abstracts/search?q=Renata%20Ochocka"> Renata Ochocka</a>, <a href="https://publications.waset.org/abstracts/search?q=Bozena%20Zabiegala"> Bozena Zabiegala</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Luczkiewicz"> Maria Luczkiewicz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rhododendron tomentosum (formerly Ledum palustre), an evergreen shrub grows in peaty soils in northern Europe, Asia and North America. In Poland, it is classified as an endangered species not only due to the drainage of wetlands, but also to the excessive collection of this repellent plant by human. The other valuable biological properties of R. tomentosum, used for years in folk medicine, include anti-inflammatory, analgesic and anti-microbial activity, conditioned by the essential oil content. Taking into account the importance of biodiversity and the potential therapeutic application, it was decided to establish, for the first time, the micropropagation protocol for R. tomentosum, for ex-situ conservation of this endangered species as well as to obtain the continuous source of in vivo and in-vitro plant material for further studies. This object was achieved by the selection of the explant and the media, which were modified within the scope of mineral composition, sugar content, pH and the growth regulators. As a result, the four-stage micropropagation protocol for R. tomentosum was specified, including shoot multiplication, elongation, rooting and ex-vitro adaptation. The genetic identification of the examined species and the compatibility of progeny plants with maternal ones was tested with molecular biology methods. Moreover, during the research process, the chemical composition of initial and regenerated plant and in vitro shoots was controlled in terms of volatile fraction by phytochemical analysis (GC and TLC methods). The correctness of the micropropagation procedure was confirmed by both types of studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ex%20situ%20conservation" title="ex situ conservation">ex situ conservation</a>, <a href="https://publications.waset.org/abstracts/search?q=Ledum%20palustre" title=" Ledum palustre"> Ledum palustre</a>, <a href="https://publications.waset.org/abstracts/search?q=micropropagation" title=" micropropagation"> micropropagation</a>, <a href="https://publications.waset.org/abstracts/search?q=Rhododendron%20tomentosum" title=" Rhododendron tomentosum"> Rhododendron tomentosum</a> </p> <a href="https://publications.waset.org/abstracts/25285/micropropagation-of-rhododendron-tomentosum-ledum-palustre-an-endangered-plant-of-scientific-interest-as-the-example-of-ex-situ-conservation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25285.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">490</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4310</span> A High Content Screening Platform for the Accurate Prediction of Nephrotoxicity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sijing%20Xiong">Sijing Xiong</a>, <a href="https://publications.waset.org/abstracts/search?q=Ran%20Su"> Ran Su</a>, <a href="https://publications.waset.org/abstracts/search?q=Lit-Hsin%20Loo"> Lit-Hsin Loo</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniele%20Zink"> Daniele Zink</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The kidney is a major target for toxic effects of drugs, industrial and environmental chemicals and other compounds. Typically, nephrotoxicity is detected late during drug development, and regulatory animal models could not solve this problem. Validated or accepted in silico or in vitro methods for the prediction of nephrotoxicity are not available. We have established the first and currently only pre-validated in vitro models for the accurate prediction of nephrotoxicity in humans and the first predictive platforms based on renal cells derived from human pluripotent stem cells. In order to further improve the efficiency of our predictive models, we recently developed a high content screening (HCS) platform. This platform employed automated imaging in combination with automated quantitative phenotypic profiling and machine learning methods. 129 image-based phenotypic features were analyzed with respect to their predictive performance in combination with 44 compounds with different chemical structures that included drugs, environmental and industrial chemicals and herbal and fungal compounds. The nephrotoxicity of these compounds in humans is well characterized. A combination of chromatin and cytoskeletal features resulted in high predictivity with respect to nephrotoxicity in humans. Test balanced accuracies of 82% or 89% were obtained with human primary or immortalized renal proximal tubular cells, respectively. Furthermore, our results revealed that a DNA damage response is commonly induced by different PTC-toxicants with diverse chemical structures and injury mechanisms. Together, the results show that the automated HCS platform allows efficient and accurate nephrotoxicity prediction for compounds with diverse chemical structures. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=high%20content%20screening" title="high content screening">high content screening</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20models" title=" in vitro models"> in vitro models</a>, <a href="https://publications.waset.org/abstracts/search?q=nephrotoxicity" title=" nephrotoxicity"> nephrotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=toxicity%20prediction" title=" toxicity prediction"> toxicity prediction</a> </p> <a href="https://publications.waset.org/abstracts/42832/a-high-content-screening-platform-for-the-accurate-prediction-of-nephrotoxicity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42832.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">313</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4309</span> Clinical Presentation and Immune Response to Intramammary Infection of Holstein-Friesian Heifers with Isolates from Two Staphylococcus aureus Lineages</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dagmara%20A.%20Niedziela">Dagmara A. Niedziela</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20P.%20Murphy"> Mark P. Murphy</a>, <a href="https://publications.waset.org/abstracts/search?q=Orla%20M.%20Keane"> Orla M. Keane</a>, <a href="https://publications.waset.org/abstracts/search?q=Finola%20C.%20Leonard"> Finola C. Leonard</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Staphylococcus aureus is the most frequent cause of clinical and subclinical bovine mastitis in Ireland. Mastitis caused by S. aureus is often chronic and tends to recur after antibiotic treatment. This may be due to several virulence factors, including attributes that enable the bacterium to internalize into bovine mammary epithelial cells, where it may evade antibiotic treatment, or evade the host immune response. Four bovine-adapted lineages (CC71, CC97, CC151 and ST136) were identified among a collection of Irish S. aureus mastitis isolates. Genotypic variation of mastitis-causing strains may contribute to different presentations of the disease, including differences in milk somatic cell count (SCC), the main method of mastitis detection. The objective of this study was to investigate the influence of bacterial strain and lineage on host immune response, by employing cell culture methods in vitro as well as an in vivo infection model. Twelve bovine adapted S. aureus strains were examined for internalization into bovine mammary epithelial cells (bMEC) and their ability to induce an immune response from bMEC (using qPCR and ELISA). In vitro studies found differences in a variety of virulence traits between the lineages. Strains from lineages CC97 and CC71 internalized more efficiently into bovine mammary epithelial cells (bMEC) than CC151 and ST136. CC97 strains also induced immune genes in bMEC more strongly than strains from the other 3 lineages. One strain each of CC151 and CC97 that differed in their ability to cause an immune response in bMEC were selected on the basis of the above in vitro experiments. Fourteen first-lactation Holstein-Friesian cows were purchased from 2 farms on the basis of low SCC (less than 50 000 cells/ml) and infection free status. Seven cows were infected with 1.73 x 102 c.f.u. of the CC97 strain (Group 1) and another seven with 5.83 x 102 c.f.u. of the CC151 strain (Group 2). The contralateral quarter of each cow was inoculated with PBS (vehicle). Clinical signs of infection (temperature, milk and udder appearance, milk yield) were monitored for 30 days. Blood and milk samples were taken to determine bacterial counts in milk, SCC, white blood cell populations and cytokines. Differences in disease presentation in vivo between groups were observed, with two animals from Group 2 developing clinical mastitis and requiring antibiotic treatment, while one animal from Group 1 did not develop an infection for the duration of the study. Fever (temperature > 39.5⁰C) was observed in 3 animals from Group 2 and in none from Group 1. Significant differences in SCC and bacterial load between groups were observed in the initial stages of infection (week 1). Data is also being collected on cytokines and chemokines secreted during the course of infection. The results of this study suggest that a strain from lineage CC151 may cause more severe clinical mastitis, while a strain from lineage CC97 may cause mild, subclinical mastitis. Diversity between strains of S. aureus may therefore influence the clinical presentation of mastitis, which in turn may influence disease detection and treatment needs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bovine%20mastitis" title="Bovine mastitis">Bovine mastitis</a>, <a href="https://publications.waset.org/abstracts/search?q=host%20immune%20response" title=" host immune response"> host immune response</a>, <a href="https://publications.waset.org/abstracts/search?q=host-pathogen%20interactions" title=" host-pathogen interactions"> host-pathogen interactions</a>, <a href="https://publications.waset.org/abstracts/search?q=Staphylococcus%20aureus" title=" Staphylococcus aureus"> Staphylococcus aureus</a> </p> <a href="https://publications.waset.org/abstracts/86932/clinical-presentation-and-immune-response-to-intramammary-infection-of-holstein-friesian-heifers-with-isolates-from-two-staphylococcus-aureus-lineages" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86932.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">157</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4308</span> Functional Analysis of Thyroid Peroxidase (TPO) Gene Mutations Detected in Patients with Thyroid Dyshormonogenesis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Biswabandhu%20Bankura">Biswabandhu Bankura</a>, <a href="https://publications.waset.org/abstracts/search?q=Srikanta%20Guria"> Srikanta Guria</a>, <a href="https://publications.waset.org/abstracts/search?q=Madhusudan%20Das"> Madhusudan Das</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: Thyroid peroxidase (TPO) is the key enzyme in the biosynthesis of thyroid hormones. We aimed to identify the spectrum of mutations in the TPO gene leading to hypothyroidism in the population of West Bengal to establish the genetic etiology of the disease. Methods: 200 hypothyroid patients (case) and their corresponding sex and age matched 200 normal individuals (control) were screened depending on their clinical manifestations. Genomic DNA was isolated from peripheral blood samples and TPO gene (Exon 7 to Exon 14) was amplified by PCR. The PCR products were subjected to sequencing to identify mutations. Results: Single nucleotide changes such as Glu 641 Lys, Asp 668 Asn, Thr 725 Pro, Asp 620 Asn, Ser 398 Thr, and Ala 373 Ser were found. Changes in the TPO were assayed in vitro to compare mutant and wild-type activities. Five mutants were enzymatically inactive in the guaiacol and iodide assays. This is a strong indication that the mutations are present at crucial positions of the TPO gene, resulting in inactivated TPO. Key Findings: The results of this study may help to develop a genetic screening protocol for goiter and hypothyroidism in the population of West Bengal. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=thyroid%20peroxidase" title="thyroid peroxidase">thyroid peroxidase</a>, <a href="https://publications.waset.org/abstracts/search?q=hypothyroidism" title=" hypothyroidism"> hypothyroidism</a>, <a href="https://publications.waset.org/abstracts/search?q=mutation" title=" mutation"> mutation</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20assay" title=" in vitro assay"> in vitro assay</a>, <a href="https://publications.waset.org/abstracts/search?q=transfection" title=" transfection"> transfection</a> </p> <a href="https://publications.waset.org/abstracts/20470/functional-analysis-of-thyroid-peroxidase-tpo-gene-mutations-detected-in-patients-with-thyroid-dyshormonogenesis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20470.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">345</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4307</span> Functional Analysis of Thyroid Peroxidase Gene Mutations Detected in Patients with Thyroid Dyshormonogenesis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Biswabandhu%20Bankura">Biswabandhu Bankura</a>, <a href="https://publications.waset.org/abstracts/search?q=Srikanta%20Guria"> Srikanta Guria</a>, <a href="https://publications.waset.org/abstracts/search?q=Madhusudan%20Das"> Madhusudan Das</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: Thyroid peroxidase (TPO) is the key enzyme in the biosynthesis of thyroid hormones. We aimed to identify the spectrum of mutations in the TPO gene leading to hypothyroidism in the population of West Bengal to establish the genetic etiology of the disease. Methods: 200 hypothyroid patients (case) and their corresponding sex and age matched 200 normal individuals (control) were screened depending on their clinical manifestations. Genomic DNA was isolated from peripheral blood samples and TPO gene (Exon 7 to Exon 14) was amplified by PCR. The PCR products were subjected to sequencing to identify mutations. Results: Single nucleotide changes such as Glu 641 Lys, Asp 668 Asn, Thr 725 Pro, Asp 620 Asn, Ser 398 Thr, and Ala 373 Ser were found. Changes in the TPO were assayed in vitro to compare mutant and wild-type activities. Five mutants were enzymatically inactive in the guaiacol and iodide assays. This is a strong indication that the mutations are present at crucial positions of the TPO gene, resulting in inactivated TPO. Key Findings: The results of this study may help to develop a genetic screening protocol for goiter and hypothyroidism in the population of West Bengal. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=thyroid%20peroxidase" title="thyroid peroxidase">thyroid peroxidase</a>, <a href="https://publications.waset.org/abstracts/search?q=hypothyroidism" title=" hypothyroidism"> hypothyroidism</a>, <a href="https://publications.waset.org/abstracts/search?q=mutation" title=" mutation"> mutation</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20assay" title=" in vitro assay"> in vitro assay</a>, <a href="https://publications.waset.org/abstracts/search?q=transfection" title=" transfection"> transfection</a> </p> <a href="https://publications.waset.org/abstracts/19059/functional-analysis-of-thyroid-peroxidase-gene-mutations-detected-in-patients-with-thyroid-dyshormonogenesis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19059.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">335</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4306</span> Influence of Tactile Symbol Size on Its Perceptibility in Consideration of Effect of Aging</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20Nishimura">T. Nishimura</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Doi"> K. Doi</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Fujimoto"> H. Fujimoto</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Wada"> T. Wada</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We conducted perception experiments on tactile symbols to elucidate the impact of the size of these letters on the level of perceptibility. This study was based on the accessible design perspective and aimed at expanding the availability of tactile symbols for the visually impaired who are unable to read Braille characters. In particular, this study targeted people with acquired visual impairments as users of the tactile symbols. The subjects (young and elderly individuals) in this study had normal vision. They were asked to participate in the experiments to identify tactile symbols while unable to see their hand during the experiments. This study investigated the relation between the size and perceptibility of tactile symbols based on an examination using test pieces of these letters in different sizes. The results revealed that the error rates for both young and elderly subjects converged to almost 0% when 12 mm size tactile symbols were used. The findings also showed that the error rate was low and subjects could identify the symbols in 5 s when 16 mm size tactile symbols were introduced. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=accessible%20design" title="accessible design">accessible design</a>, <a href="https://publications.waset.org/abstracts/search?q=tactile%20sense" title=" tactile sense"> tactile sense</a>, <a href="https://publications.waset.org/abstracts/search?q=tactile%20symbols" title=" tactile symbols"> tactile symbols</a>, <a href="https://publications.waset.org/abstracts/search?q=bioinformatic" title=" bioinformatic"> bioinformatic</a> </p> <a href="https://publications.waset.org/abstracts/13010/influence-of-tactile-symbol-size-on-its-perceptibility-in-consideration-of-effect-of-aging" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13010.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">351</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=7" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=1">1</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=2">2</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=5">5</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=6">6</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=7">7</a></li> <li class="page-item active"><span class="page-link">8</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=9">9</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=10">10</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=11">11</a></li> <li class="page-item disabled"><span class="page-link">...</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=151">151</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=152">152</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=in%20vitro%20experiments&page=9" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">© 2024 World Academy of Science, Engineering and Technology</div> </div> </footer> <a href="javascript:" id="return-to-top"><i class="fas fa-arrow-up"></i></a> <div class="modal" id="modal-template"> <div class="modal-dialog"> <div class="modal-content"> <div class="row m-0 mt-1"> <div class="col-md-12"> <button type="button" class="close" data-dismiss="modal" aria-label="Close"><span aria-hidden="true">×</span></button> </div> </div> <div class="modal-body"></div> </div> </div> </div> <script src="https://cdn.waset.org/static/plugins/jquery-3.3.1.min.js"></script> <script src="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/js/bootstrap.bundle.min.js"></script> <script src="https://cdn.waset.org/static/js/site.js?v=150220211556"></script> <script> jQuery(document).ready(function() { /*jQuery.get("https://publications.waset.org/xhr/user-menu", function (response) { jQuery('#mainNavMenu').append(response); });*/ jQuery.get({ url: "https://publications.waset.org/xhr/user-menu", cache: false }).then(function(response){ jQuery('#mainNavMenu').append(response); }); }); </script> </body> </html>