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overflow: hidden; text-overflow: ellipsis; -webkit-line-clamp: 3; -webkit-box-orient: vertical; }</style><div class="col-xs-12 clearfix"><div class="u-floatLeft"><h1 class="PageHeader-title u-m0x u-fs30">Calcium</h1><div class="u-tcGrayDark">12,991 Followers</div><div class="u-tcGrayDark u-mt2x">Recent papers in <b>Calcium</b></div></div></div></div></div></div><div class="TabbedNavigation"><div class="container"><div class="row"><div class="col-xs-12 clearfix"><ul class="nav u-m0x u-p0x list-inline u-displayFlex"><li class="active"><a href="https://www.academia.edu/Documents/in/Calcium">Top Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Calcium/MostCited">Most Cited Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Calcium/MostDownloaded">Most Downloaded Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Calcium/MostRecent">Newest Papers</a></li><li><a class="" href="https://www.academia.edu/People/Calcium">People</a></li></ul></div><style type="text/css">ul.nav{flex-direction:row}@media(max-width: 567px){ul.nav{flex-direction:column}.TabbedNavigation li{max-width:100%}.TabbedNavigation li.active{background-color:var(--background-grey, #dddde2)}.TabbedNavigation li.active:before,.TabbedNavigation li.active:after{display:none}}</style></div></div></div><div class="container"><div class="row"><div class="col-xs-12"><div class="u-displayFlex"><div class="u-flexGrow1"><div class="works"><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_34351459" data-work_id="34351459" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/34351459/Characteristics_of_Spontaneous_Activity_in_the_Bladder_Trigone">Characteristics of Spontaneous Activity in the Bladder Trigone</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: During bladder filling, the trigone contracts help keep the ureteral orifices open and the bladder neck shut. The trigone generates spontaneous activity as well as responding to neuromuscular transmitters, but the relationship... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_34351459" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: During bladder filling, the trigone contracts help keep the ureteral orifices open and the bladder neck shut. The trigone generates spontaneous activity as well as responding to neuromuscular transmitters, but the relationship between these phenomena are unclear. Objectives: To characterise the cellular mechanisms that regulate and modify spontaneous activity in trigone smooth muscle. Design, setting, and participants: Muscle strips from the superficial trigone of male guinea-pigs were used for tension experiments and immunofluorescent studies. Measurements: In isolated trigonal cells, intracellular Ca 2+ was measured by epifluorescence microscopy using the fluorescent Ca 2+ indicator Fura-2. Results and limitations: Spontaneous intracellular Ca 2+ transients and contractions were observed in trigonal single cells and strips and were significantly higher compared to the bladder dome. Ca-free superfusate and verapamil terminated spontaneity. T-type Ca 2+ channel block with NiCl 2 depressed slightly Ca 2+ transients but not spontaneous contractions. Neither the BK Ca channel blocker iberiotoxin nor the SK Ca channel blocker apamin had any effect on single cell activity. By contrast, the Cl À channel blocker niflumic acid attenuated significantly both Ca 2+ transients and muscle contractions. Agonist stimulation (carbachol, phenylephrine) up-regulated activity. Gap junction labelling (Cx43) was approximately 5 times denser in the trigone than in detrusor smooth muscle. The gap junction blocker 18-ß-glycyrrhetinic acid modulated spontaneous contractions in the trigone but not in the bladder dome. Conclusions: Trigone myocytes employ membrane L-type-Ca 2+ channels and Cl À channels to generate spontaneous activity. Intercellular electrical coupling ensures its propagation and, thus, sustains contraction of the whole trigone.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/34351459" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a8efddff7557e03c9e974bcbb97aa262" rel="nofollow" data-download="{"attachment_id":54243546,"asset_id":34351459,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/54243546/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10264" href="https://imperial.academia.edu/RashedaChowdhury">Rasheda Chowdhury</a><script data-card-contents-for-user="10264" type="text/json">{"id":10264,"first_name":"Rasheda","last_name":"Chowdhury","domain_name":"imperial","page_name":"RashedaChowdhury","display_name":"Rasheda Chowdhury","profile_url":"https://imperial.academia.edu/RashedaChowdhury?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_34351459 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="34351459"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 34351459, container: ".js-paper-rank-work_34351459", }); 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$(".js-view-count[data-work-id=34351459]").text(description); $(".js-view-count-work_34351459").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_34351459").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="34351459"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="622" rel="nofollow" href="https://www.academia.edu/Documents/in/Nephrology">Nephrology</a>, <script data-card-contents-for-ri="622" type="text/json">{"id":622,"name":"Nephrology","url":"https://www.academia.edu/Documents/in/Nephrology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="653" rel="nofollow" href="https://www.academia.edu/Documents/in/Urology">Urology</a>, <script data-card-contents-for-ri="653" type="text/json">{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="65171" rel="nofollow" href="https://www.academia.edu/Documents/in/Smooth_muscle">Smooth muscle</a><script data-card-contents-for-ri="65171" type="text/json">{"id":65171,"name":"Smooth muscle","url":"https://www.academia.edu/Documents/in/Smooth_muscle?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=34351459]'), work: {"id":34351459,"title":"Characteristics of Spontaneous Activity in the Bladder Trigone","created_at":"2017-08-25T06:17:04.430-07:00","url":"https://www.academia.edu/34351459/Characteristics_of_Spontaneous_Activity_in_the_Bladder_Trigone?f_ri=9534","dom_id":"work_34351459","summary":"Background: During bladder filling, the trigone contracts help keep the ureteral orifices open and the bladder neck shut. The trigone generates spontaneous activity as well as responding to neuromuscular transmitters, but the relationship between these phenomena are unclear. Objectives: To characterise the cellular mechanisms that regulate and modify spontaneous activity in trigone smooth muscle. Design, setting, and participants: Muscle strips from the superficial trigone of male guinea-pigs were used for tension experiments and immunofluorescent studies. Measurements: In isolated trigonal cells, intracellular Ca 2+ was measured by epifluorescence microscopy using the fluorescent Ca 2+ indicator Fura-2. Results and limitations: Spontaneous intracellular Ca 2+ transients and contractions were observed in trigonal single cells and strips and were significantly higher compared to the bladder dome. Ca-free superfusate and verapamil terminated spontaneity. T-type Ca 2+ channel block with NiCl 2 depressed slightly Ca 2+ transients but not spontaneous contractions. Neither the BK Ca channel blocker iberiotoxin nor the SK Ca channel blocker apamin had any effect on single cell activity. By contrast, the Cl À channel blocker niflumic acid attenuated significantly both Ca 2+ transients and muscle contractions. Agonist stimulation (carbachol, phenylephrine) up-regulated activity. Gap junction labelling (Cx43) was approximately 5 times denser in the trigone than in detrusor smooth muscle. The gap junction blocker 18-ß-glycyrrhetinic acid modulated spontaneous contractions in the trigone but not in the bladder dome. Conclusions: Trigone myocytes employ membrane L-type-Ca 2+ channels and Cl À channels to generate spontaneous activity. Intercellular electrical coupling ensures its propagation and, thus, sustains contraction of the whole trigone.","downloadable_attachments":[{"id":54243546,"asset_id":34351459,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":10264,"first_name":"Rasheda","last_name":"Chowdhury","domain_name":"imperial","page_name":"RashedaChowdhury","display_name":"Rasheda Chowdhury","profile_url":"https://imperial.academia.edu/RashedaChowdhury?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":622,"name":"Nephrology","url":"https://www.academia.edu/Documents/in/Nephrology?f_ri=9534","nofollow":true},{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":65171,"name":"Smooth muscle","url":"https://www.academia.edu/Documents/in/Smooth_muscle?f_ri=9534","nofollow":true},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":419061,"name":"Urinary System","url":"https://www.academia.edu/Documents/in/Urinary_System?f_ri=9534"},{"id":485556,"name":"Guinea Pig","url":"https://www.academia.edu/Documents/in/Guinea_Pig?f_ri=9534"},{"id":556021,"name":"Muscle contraction","url":"https://www.academia.edu/Documents/in/Muscle_contraction?f_ri=9534"},{"id":633766,"name":"Sediment Characteristic","url":"https://www.academia.edu/Documents/in/Sediment_Characteristic?f_ri=9534"},{"id":783432,"name":"Biological activity","url":"https://www.academia.edu/Documents/in/Biological_activity?f_ri=9534"},{"id":1902592,"name":"Urinary Bladder","url":"https://www.academia.edu/Documents/in/Urinary_Bladder?f_ri=9534"},{"id":2111545,"name":"Spontaneous Activity","url":"https://www.academia.edu/Documents/in/Spontaneous_Activity?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_51134159" data-work_id="51134159" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/51134159/Dose_ranging_effects_of_citrulline_administration_on_plasma_amino_acids_and_hormonal_patterns_in_healthy_subjects_the_Citrudose_pharmacokinetic_study">Dose-ranging effects of citrulline administration on plasma amino acids and hormonal patterns in healthy subjects: the Citrudose pharmacokinetic study</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Previous experimental studies have highlighted that citrulline (CIT) could be a promising pharmaconutrient. However, its pharmacokinetic characteristics and tolerance to loading have not been studied to date. The objective was to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_51134159" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Previous experimental studies have highlighted that citrulline (CIT) could be a promising pharmaconutrient. However, its pharmacokinetic characteristics and tolerance to loading have not been studied to date. The objective was to characterise the plasma kinetics of CIT in a multiple-dosing study design and to assess the effect of CIT intake on the concentrations of other plasma amino acids (AA). The effects of CIT loading on anabolic hormones were also determined. Eight fasting healthy males underwent four separate oral loading tests (2, 5, 10 or 15 g CIT) in random order. Blood was drawn ten times over an 8 h period for measurement of plasma AA, insulin and growth hormone (Gh). Urine samples were collected before CIT administration and over the next 24 h. None of the subjects experienced side effects whatever the CIT dose. Concerning AA, only CIT, ornithine (ORN) and arginine (ARG) plasma concentrations were affected (maximum concentration 146 (sem 8) to 303 (sem 11) μmol/l (ARG) a...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/51134159" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="328ac131ddd5ef6902ebc76619f3cd0d" rel="nofollow" data-download="{"attachment_id":68976220,"asset_id":51134159,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/68976220/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39617635" href="https://independent.academia.edu/ChristopheMoinard">Christophe Moinard</a><script data-card-contents-for-user="39617635" type="text/json">{"id":39617635,"first_name":"Christophe","last_name":"Moinard","domain_name":"independent","page_name":"ChristopheMoinard","display_name":"Christophe Moinard","profile_url":"https://independent.academia.edu/ChristopheMoinard?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_51134159 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="51134159"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 51134159, container: ".js-paper-rank-work_51134159", }); });</script></li><li class="js-percentile-work_51134159 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 51134159; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_51134159"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_51134159 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="51134159"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 51134159; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=51134159]").text(description); $(".js-view-count-work_51134159").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_51134159").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="51134159"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="7324" rel="nofollow" href="https://www.academia.edu/Documents/in/British">British</a>, <script data-card-contents-for-ri="7324" type="text/json">{"id":7324,"name":"British","url":"https://www.academia.edu/Documents/in/British?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="29980" rel="nofollow" href="https://www.academia.edu/Documents/in/Animal_Production">Animal Production</a>, <script data-card-contents-for-ri="29980" type="text/json">{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="52562" rel="nofollow" href="https://www.academia.edu/Documents/in/Growth_Hormone">Growth Hormone</a><script data-card-contents-for-ri="52562" type="text/json">{"id":52562,"name":"Growth Hormone","url":"https://www.academia.edu/Documents/in/Growth_Hormone?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=51134159]'), work: {"id":51134159,"title":"Dose-ranging effects of citrulline administration on plasma amino acids and hormonal patterns in healthy subjects: the Citrudose pharmacokinetic study","created_at":"2021-09-01T04:14:21.790-07:00","url":"https://www.academia.edu/51134159/Dose_ranging_effects_of_citrulline_administration_on_plasma_amino_acids_and_hormonal_patterns_in_healthy_subjects_the_Citrudose_pharmacokinetic_study?f_ri=9534","dom_id":"work_51134159","summary":"Previous experimental studies have highlighted that citrulline (CIT) could be a promising pharmaconutrient. However, its pharmacokinetic characteristics and tolerance to loading have not been studied to date. The objective was to characterise the plasma kinetics of CIT in a multiple-dosing study design and to assess the effect of CIT intake on the concentrations of other plasma amino acids (AA). The effects of CIT loading on anabolic hormones were also determined. Eight fasting healthy males underwent four separate oral loading tests (2, 5, 10 or 15 g CIT) in random order. Blood was drawn ten times over an 8 h period for measurement of plasma AA, insulin and growth hormone (Gh). Urine samples were collected before CIT administration and over the next 24 h. None of the subjects experienced side effects whatever the CIT dose. Concerning AA, only CIT, ornithine (ORN) and arginine (ARG) plasma concentrations were affected (maximum concentration 146 (sem 8) to 303 (sem 11) μmol/l (ARG) a...","downloadable_attachments":[{"id":68976220,"asset_id":51134159,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39617635,"first_name":"Christophe","last_name":"Moinard","domain_name":"independent","page_name":"ChristopheMoinard","display_name":"Christophe Moinard","profile_url":"https://independent.academia.edu/ChristopheMoinard?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":7324,"name":"British","url":"https://www.academia.edu/Documents/in/British?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production?f_ri=9534","nofollow":true},{"id":52562,"name":"Growth Hormone","url":"https://www.academia.edu/Documents/in/Growth_Hormone?f_ri=9534","nofollow":true},{"id":71294,"name":"Kidney","url":"https://www.academia.edu/Documents/in/Kidney?f_ri=9534"},{"id":71300,"name":"Blood Glucose","url":"https://www.academia.edu/Documents/in/Blood_Glucose?f_ri=9534"},{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin?f_ri=9534"},{"id":203750,"name":"Dose","url":"https://www.academia.edu/Documents/in/Dose?f_ri=9534"},{"id":295928,"name":"Amino Acids","url":"https://www.academia.edu/Documents/in/Amino_Acids?f_ri=9534"},{"id":413194,"name":"Analysis of Variance","url":"https://www.academia.edu/Documents/in/Analysis_of_Variance?f_ri=9534"},{"id":555120,"name":"Arginine","url":"https://www.academia.edu/Documents/in/Arginine?f_ri=9534"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=9534"},{"id":879006,"name":"Citrulline","url":"https://www.academia.edu/Documents/in/Citrulline?f_ri=9534"},{"id":1142759,"name":"Healthy Subjects","url":"https://www.academia.edu/Documents/in/Healthy_Subjects?f_ri=9534"},{"id":1309706,"name":"Area Under Curve","url":"https://www.academia.edu/Documents/in/Area_Under_Curve?f_ri=9534"},{"id":1438730,"name":"Creatinine","url":"https://www.academia.edu/Documents/in/Creatinine?f_ri=9534"},{"id":1592853,"name":"Drug Tolerance","url":"https://www.academia.edu/Documents/in/Drug_Tolerance?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_30289349" data-work_id="30289349" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/30289349/Altered_Ca2_Kinetics_Associated_with_%CE%B1_Actinin_3_Deficiency_May_Explain_Positive_Selection_for_ACTN3_Null_Allele_in_Human_Evolution">Altered Ca2+ Kinetics Associated with α-Actinin-3 Deficiency May Explain Positive Selection for ACTN3 Null Allele in Human Evolution</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Over 1.5 billion people lack the skeletal muscle fast-twitch fibre protein α-actinin-3 due to homozygosity for a common null polymorphism (R577X) in the ACTN3 gene. α-Actinin-3 deficiency is detrimental to sprint performance in elite... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_30289349" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Over 1.5 billion people lack the skeletal muscle fast-twitch fibre protein α-actinin-3 due to homozygosity for a common null polymorphism (R577X) in the ACTN3 gene. α-Actinin-3 deficiency is detrimental to sprint performance in elite athletes and beneficial to endurance activities. In the human genome, it is very difficult to find single-gene loss-of-function variants that bear signatures of positive selection, yet intriguingly, the ACTN3 null variant has undergone strong positive selection during recent evolution, appearing to provide a survival advantage where food resources are scarce and climate is cold. We have previously demonstrated that α-actinin-3 deficiency in the Actn3 KO mouse results in a shift in fast-twitch fibres towards oxidative metabolism, which would be more &quot;energy efficient&quot; in famine, and beneficial to endurance performance. Prolonged exposure to cold can also induce changes in skeletal muscle similar to those observed with endurance training, and ch...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/30289349" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cb5d28b9a5745fc791ad0b65652cd11a" rel="nofollow" data-download="{"attachment_id":50750533,"asset_id":30289349,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50750533/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="57730393" href="https://unimelb.academia.edu/PeterHouweling">Peter Houweling</a><script data-card-contents-for-user="57730393" type="text/json">{"id":57730393,"first_name":"Peter","last_name":"Houweling","domain_name":"unimelb","page_name":"PeterHouweling","display_name":"Peter Houweling","profile_url":"https://unimelb.academia.edu/PeterHouweling?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_30289349 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="30289349"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 30289349, container: ".js-paper-rank-work_30289349", }); 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$(".js-view-count[data-work-id=30289349]").text(description); $(".js-view-count-work_30289349").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_30289349").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="30289349"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="156" rel="nofollow" href="https://www.academia.edu/Documents/in/Genetics">Genetics</a>, <script data-card-contents-for-ri="156" type="text/json">{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4228" rel="nofollow" href="https://www.academia.edu/Documents/in/Skeletal_muscle_biology">Skeletal muscle biology</a>, <script data-card-contents-for-ri="4228" type="text/json">{"id":4228,"name":"Skeletal muscle biology","url":"https://www.academia.edu/Documents/in/Skeletal_muscle_biology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a><script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=30289349]'), work: {"id":30289349,"title":"Altered Ca2+ Kinetics Associated with α-Actinin-3 Deficiency May Explain Positive Selection for ACTN3 Null Allele in Human Evolution","created_at":"2016-12-06T11:21:32.682-08:00","url":"https://www.academia.edu/30289349/Altered_Ca2_Kinetics_Associated_with_%CE%B1_Actinin_3_Deficiency_May_Explain_Positive_Selection_for_ACTN3_Null_Allele_in_Human_Evolution?f_ri=9534","dom_id":"work_30289349","summary":"Over 1.5 billion people lack the skeletal muscle fast-twitch fibre protein α-actinin-3 due to homozygosity for a common null polymorphism (R577X) in the ACTN3 gene. α-Actinin-3 deficiency is detrimental to sprint performance in elite athletes and beneficial to endurance activities. In the human genome, it is very difficult to find single-gene loss-of-function variants that bear signatures of positive selection, yet intriguingly, the ACTN3 null variant has undergone strong positive selection during recent evolution, appearing to provide a survival advantage where food resources are scarce and climate is cold. We have previously demonstrated that α-actinin-3 deficiency in the Actn3 KO mouse results in a shift in fast-twitch fibres towards oxidative metabolism, which would be more \u0026quot;energy efficient\u0026quot; in famine, and beneficial to endurance performance. Prolonged exposure to cold can also induce changes in skeletal muscle similar to those observed with endurance training, and ch...","downloadable_attachments":[{"id":50750533,"asset_id":30289349,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":57730393,"first_name":"Peter","last_name":"Houweling","domain_name":"unimelb","page_name":"PeterHouweling","display_name":"Peter Houweling","profile_url":"https://unimelb.academia.edu/PeterHouweling?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=9534","nofollow":true},{"id":4228,"name":"Skeletal muscle biology","url":"https://www.academia.edu/Documents/in/Skeletal_muscle_biology?f_ri=9534","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":15719,"name":"Mitochondria","url":"https://www.academia.edu/Documents/in/Mitochondria?f_ri=9534"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534"},{"id":146042,"name":"Weather","url":"https://www.academia.edu/Documents/in/Weather?f_ri=9534"},{"id":191815,"name":"Biological evolution","url":"https://www.academia.edu/Documents/in/Biological_evolution?f_ri=9534"},{"id":453528,"name":"Acclimatization","url":"https://www.academia.edu/Documents/in/Acclimatization?f_ri=9534"},{"id":558653,"name":"PLoS Genetics","url":"https://www.academia.edu/Documents/in/PLoS_Genetics?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_23215110" data-work_id="23215110" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/23215110/Vitamin_D_status_and_response_to_treatment_in_post_menopausal_osteoporosis">Vitamin D status and response to treatment in post-menopausal osteoporosis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Treatment with anti-resorptive agents over 13 months was associated with for three to fivefold lower bone mineral density changes and 1.5-fold increased risk of incidence fracture in vitamin D insufficient as compared to vitamin D... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23215110" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Treatment with anti-resorptive agents over 13 months was associated with for three to fivefold lower bone mineral density changes and 1.5-fold increased risk of incidence fracture in vitamin D insufficient as compared to vitamin D repleted postmenopausal osteoporotic women. Introduction Several drugs were registered for the treatment of osteoporosis on the basis of clinical trials in which vitamin D repletion was a pre-requisite inclusion criteria and vitamin D supplements were used as adjunctive therapy. However, in routine clinical practice these supplements are not consistently recommended. Methods We studied 1515 women with postmenopausal osteoporosis under treatment with anti-resorbing agents (alendronate, risedronate, raloxifene) for 13.1 months with an adherence > 75%. The patients were classified as vitamin D deficient (N=514) or vitamin D repleted (N=1001) according to risk factors (N=1062) or the level of 25(OH) vitamin D [25(OH)D] above or below 50 nmol/l (N=453). Results Vitamin D deficient and vitamin D repleted subjects differed significantly for annualized spine and hip bone mineral density (BMD) changes adjusted for all available confounding factors (type of treatment, age, global calcium intake, baseline BMD values). One hundred fifty one patients suffered from a new incident clinical fracture. The adjusted odds ratio for incident fractures in vitamin D deficient as compared to vitamin D repleted women was 1.77 (1.20 -2.59, 95% CI; p=0.004). Conclusions Optimal vitamin D repletion seems to be necessary to maximize the response to anti-resorbers in terms of both BMD changes and anti-fracture efficacy.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23215110" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b7749f5d5325b5b69c2b370e8a30a270" rel="nofollow" data-download="{"attachment_id":43704884,"asset_id":23215110,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43704884/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="45058036" href="https://independent.academia.edu/GerolamoBianchi">Gerolamo Bianchi</a><script data-card-contents-for-user="45058036" type="text/json">{"id":45058036,"first_name":"Gerolamo","last_name":"Bianchi","domain_name":"independent","page_name":"GerolamoBianchi","display_name":"Gerolamo Bianchi","profile_url":"https://independent.academia.edu/GerolamoBianchi?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_23215110 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23215110"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23215110, container: ".js-paper-rank-work_23215110", }); 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$(".js-view-count[data-work-id=23215110]").text(description); $(".js-view-count-work_23215110").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_23215110").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="23215110"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">24</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4531" rel="nofollow" href="https://www.academia.edu/Documents/in/Clinical_Trial">Clinical Trial</a>, <script data-card-contents-for-ri="4531" type="text/json">{"id":4531,"name":"Clinical Trial","url":"https://www.academia.edu/Documents/in/Clinical_Trial?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="8207" rel="nofollow" href="https://www.academia.edu/Documents/in/Risk">Risk</a>, <script data-card-contents-for-ri="8207" type="text/json">{"id":8207,"name":"Risk","url":"https://www.academia.edu/Documents/in/Risk?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9531" rel="nofollow" href="https://www.academia.edu/Documents/in/Vitamin_D">Vitamin D</a>, <script data-card-contents-for-ri="9531" type="text/json">{"id":9531,"name":"Vitamin D","url":"https://www.academia.edu/Documents/in/Vitamin_D?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a><script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=23215110]'), work: {"id":23215110,"title":"Vitamin D status and response to treatment in post-menopausal osteoporosis","created_at":"2016-03-14T00:42:19.181-07:00","url":"https://www.academia.edu/23215110/Vitamin_D_status_and_response_to_treatment_in_post_menopausal_osteoporosis?f_ri=9534","dom_id":"work_23215110","summary":"Treatment with anti-resorptive agents over 13 months was associated with for three to fivefold lower bone mineral density changes and 1.5-fold increased risk of incidence fracture in vitamin D insufficient as compared to vitamin D repleted postmenopausal osteoporotic women. Introduction Several drugs were registered for the treatment of osteoporosis on the basis of clinical trials in which vitamin D repletion was a pre-requisite inclusion criteria and vitamin D supplements were used as adjunctive therapy. However, in routine clinical practice these supplements are not consistently recommended. Methods We studied 1515 women with postmenopausal osteoporosis under treatment with anti-resorbing agents (alendronate, risedronate, raloxifene) for 13.1 months with an adherence \u003e 75%. The patients were classified as vitamin D deficient (N=514) or vitamin D repleted (N=1001) according to risk factors (N=1062) or the level of 25(OH) vitamin D [25(OH)D] above or below 50 nmol/l (N=453). Results Vitamin D deficient and vitamin D repleted subjects differed significantly for annualized spine and hip bone mineral density (BMD) changes adjusted for all available confounding factors (type of treatment, age, global calcium intake, baseline BMD values). One hundred fifty one patients suffered from a new incident clinical fracture. The adjusted odds ratio for incident fractures in vitamin D deficient as compared to vitamin D repleted women was 1.77 (1.20 -2.59, 95% CI; p=0.004). Conclusions Optimal vitamin D repletion seems to be necessary to maximize the response to anti-resorbers in terms of both BMD changes and anti-fracture efficacy.","downloadable_attachments":[{"id":43704884,"asset_id":23215110,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":45058036,"first_name":"Gerolamo","last_name":"Bianchi","domain_name":"independent","page_name":"GerolamoBianchi","display_name":"Gerolamo Bianchi","profile_url":"https://independent.academia.edu/GerolamoBianchi?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4531,"name":"Clinical Trial","url":"https://www.academia.edu/Documents/in/Clinical_Trial?f_ri=9534","nofollow":true},{"id":8207,"name":"Risk","url":"https://www.academia.edu/Documents/in/Risk?f_ri=9534","nofollow":true},{"id":9531,"name":"Vitamin D","url":"https://www.academia.edu/Documents/in/Vitamin_D?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":12426,"name":"Treatment Outcome","url":"https://www.academia.edu/Documents/in/Treatment_Outcome?f_ri=9534"},{"id":44529,"name":"Activities of Daily Living","url":"https://www.academia.edu/Documents/in/Activities_of_Daily_Living?f_ri=9534"},{"id":52836,"name":"Clinical Practice","url":"https://www.academia.edu/Documents/in/Clinical_Practice?f_ri=9534"},{"id":63997,"name":"Osteoporosis","url":"https://www.academia.edu/Documents/in/Osteoporosis?f_ri=9534"},{"id":139532,"name":"Bone Density","url":"https://www.academia.edu/Documents/in/Bone_Density?f_ri=9534"},{"id":152562,"name":"Dietary Supplements","url":"https://www.academia.edu/Documents/in/Dietary_Supplements?f_ri=9534"},{"id":174502,"name":"Incidence","url":"https://www.academia.edu/Documents/in/Incidence?f_ri=9534"},{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=9534"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":272426,"name":"Alendronate","url":"https://www.academia.edu/Documents/in/Alendronate?f_ri=9534"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=9534"},{"id":437772,"name":"Odds ratio","url":"https://www.academia.edu/Documents/in/Odds_ratio?f_ri=9534"},{"id":438516,"name":"Bone Mineral Density","url":"https://www.academia.edu/Documents/in/Bone_Mineral_Density?f_ri=9534"},{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=9534"},{"id":568482,"name":"Biological markers","url":"https://www.academia.edu/Documents/in/Biological_markers?f_ri=9534"},{"id":620049,"name":"Risk Factors","url":"https://www.academia.edu/Documents/in/Risk_Factors-1?f_ri=9534"},{"id":1114577,"name":"Vitamin D Deficiency","url":"https://www.academia.edu/Documents/in/Vitamin_D_Deficiency?f_ri=9534"},{"id":1294607,"name":"Logistic Models","url":"https://www.academia.edu/Documents/in/Logistic_Models?f_ri=9534"},{"id":1399820,"name":"Bone Resorption","url":"https://www.academia.edu/Documents/in/Bone_Resorption?f_ri=9534"},{"id":2366479,"name":"Raloxifene","url":"https://www.academia.edu/Documents/in/Raloxifene?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_10676971" data-work_id="10676971" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/10676971/The_calcium_sensing_receptor_a_key_factor_in_the_pathogenesis_of_secondary_hyperparathyroidism">The calcium-sensing receptor: a key factor in the pathogenesis of secondary hyperparathyroidism</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Serum calcium levels are regulated by the action of parathyroid hormone (PTH). Major drivers of PTH hypersecretion and parathyroid cell proliferation are the hypocalcemia and hyperphosphatemia that develop in chronic kidney disease... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_10676971" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Serum calcium levels are regulated by the action of parathyroid hormone (PTH). Major drivers of PTH hypersecretion and parathyroid cell proliferation are the hypocalcemia and hyperphosphatemia that develop in chronic kidney disease patients with secondary hyperparathyroidism (SHPT) as a result of low calcitriol levels and decreased kidney function. Increased PTH production in response to systemic hypocalcemia is mediated by the calcium-sensing receptor (CaR). Furthermore, as SHPT progresses, reduced expression of CaRs and vitamin D receptors (VDRs) in hyperplastic parathyroid glands may limit the ability of calcium and calcitriol to regulate PTH secretion. Current treatment for SHPT includes the administration of vitamin D sterols and phosphate binders. Treatment with vitamin D is initially effective, but efficacy often wanes with further disease progression. The actions of vitamin D sterols are undermined by reduced expression of VDRs in the parathyroid gland. Furthermore, the calcemic and phosphatemic actions of vitamin D mean that it has the potential to exacerbate abnormal mineral metabolism, resulting in the formation of vascular calcifications. Effective new treatments for SHPT that have a positive impact on mineral metabolism are clearly</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/10676971" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a6576c0c1697944362042a9b4982170a" rel="nofollow" data-download="{"attachment_id":47211897,"asset_id":10676971,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47211897/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="26067230" href="https://independent.academia.edu/MarianoRodriguez4">Mariano Rodriguez</a><script data-card-contents-for-user="26067230" type="text/json">{"id":26067230,"first_name":"Mariano","last_name":"Rodriguez","domain_name":"independent","page_name":"MarianoRodriguez4","display_name":"Mariano Rodriguez","profile_url":"https://independent.academia.edu/MarianoRodriguez4?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_10676971 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="10676971"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 10676971, container: ".js-paper-rank-work_10676971", }); 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Major drivers of PTH hypersecretion and parathyroid cell proliferation are the hypocalcemia and hyperphosphatemia that develop in chronic kidney disease patients with secondary hyperparathyroidism (SHPT) as a result of low calcitriol levels and decreased kidney function. Increased PTH production in response to systemic hypocalcemia is mediated by the calcium-sensing receptor (CaR). Furthermore, as SHPT progresses, reduced expression of CaRs and vitamin D receptors (VDRs) in hyperplastic parathyroid glands may limit the ability of calcium and calcitriol to regulate PTH secretion. Current treatment for SHPT includes the administration of vitamin D sterols and phosphate binders. Treatment with vitamin D is initially effective, but efficacy often wanes with further disease progression. The actions of vitamin D sterols are undermined by reduced expression of VDRs in the parathyroid gland. Furthermore, the calcemic and phosphatemic actions of vitamin D mean that it has the potential to exacerbate abnormal mineral metabolism, resulting in the formation of vascular calcifications. Effective new treatments for SHPT that have a positive impact on mineral metabolism are clearly","downloadable_attachments":[{"id":47211897,"asset_id":10676971,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":26067230,"first_name":"Mariano","last_name":"Rodriguez","domain_name":"independent","page_name":"MarianoRodriguez4","display_name":"Mariano Rodriguez","profile_url":"https://independent.academia.edu/MarianoRodriguez4?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=9534","nofollow":true},{"id":8999,"name":"Kidney diseases","url":"https://www.academia.edu/Documents/in/Kidney_diseases?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":97269,"name":"Chronic Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease?f_ri=9534","nofollow":true},{"id":146046,"name":"Calcium Sensing Receptor","url":"https://www.academia.edu/Documents/in/Calcium_Sensing_Receptor?f_ri=9534"},{"id":151448,"name":"American","url":"https://www.academia.edu/Documents/in/American?f_ri=9534"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=9534"},{"id":379889,"name":"Homeostasis","url":"https://www.academia.edu/Documents/in/Homeostasis?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_31409668" data-work_id="31409668" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/31409668/The_Effects_of_Twelve_Weeks_of_Bed_Rest_on_Bone_Histology_Biochemical_Markers_of_Bone_Turnover_and_Calcium_Homeostasis_in_Eleven_Normal_Subjects">The Effects of Twelve Weeks of Bed Rest on Bone Histology, Biochemical Markers of Bone Turnover, and Calcium Homeostasis in Eleven Normal Subjects</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This study was undertaken to examine the effects of 12 weeks of skeletal unloading on parameters of calcium homeostasis, calcitropic hormones, bone histology, and biochemical markers of bone turnover in 11 normal subjects (9 men, 2 women;... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_31409668" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This study was undertaken to examine the effects of 12 weeks of skeletal unloading on parameters of calcium homeostasis, calcitropic hormones, bone histology, and biochemical markers of bone turnover in 11 normal subjects (9 men, 2 women; 34 ؎ 11 years of age). Following an ambulatory control evaluation, all subjects underwent 12 weeks of bed rest. An additional metabolic evaluation was performed after 12 days of reambulation. Bone mineral density declined at the spine (؊2.9%, p ‫؍‬ 0.092) and at the hip (؊3.8%, p ‫؍‬ 0.002 for the trochanter). Bed rest prompted a rapid, sustained, significant increase in urinary calcium and phosphorus as well as a significant increase in serum calcium. Urinary calcium increased from a pre-bed rest value of 5.3 mmol/day to values as high as 7.3 mmol/day during bed rest. Immunoreactive parathyroid hormone and serum 1,25dihydroxyvitamin D declined significantly during bed rest, although the mean values remained within normal limits. Significant changes in bone histology included a suppression of osteoblastic surface for cancellous bone (3.1 ؎ 1.3% to 1.9 ؎ 1.5%, p ‫؍‬ 0.0142) and increased bone resorption for both cancellous and cortical bone. Cortical eroded surface increased from 3.5 ؎ 1.1% to 7.3 ؎ 4.0% (p ‫؍‬ 0.018) as did active osteoclastic surface (0.2 ؎ 0.3% to 0.7 ؎ 0.7%, p ‫؍‬ 0.021). Cancellous eroded surface increased from 2.1 ؎ 1.1% to 4.7 ؎ 2.2% (p ‫؍‬ 0.002), while mean active osteoclastic surface doubled (0.2 ؎ 0.2% to 0.4 ؎ 0.3%, p ‫؍‬ 0.020). Serum biochemical markers of bone formation (osteocalcin, bone-specific alkaline phosphatase, and type I procollagen extension peptide) did not change significantly during bed rest. Urinary biochemical markers of bone resorption (hydroxyproline, deoxypyridinoline, and N-telopeptide of type I collagen) as well as a serum marker of bone resorption (type I collagen carboxytelopeptide) all demonstrated significant increases during bed rest which declined toward normal during reambulation. Thus, under the conditions of this study, the human skeleton appears to respond to unloading by a rapid and sustained increase in bone resorption and a more subtle decrease in bone formation. (J Bone Miner Res 1998;13:1594-1601)</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/31409668" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="baca69385fe7e959dc602a16e1cdb52e" rel="nofollow" data-download="{"attachment_id":51777130,"asset_id":31409668,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/51777130/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34536936" href="https://independent.academia.edu/JosephZerwekh">Joseph Zerwekh</a><script data-card-contents-for-user="34536936" type="text/json">{"id":34536936,"first_name":"Joseph","last_name":"Zerwekh","domain_name":"independent","page_name":"JosephZerwekh","display_name":"Joseph Zerwekh","profile_url":"https://independent.academia.edu/JosephZerwekh?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_31409668 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="31409668"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 31409668, container: ".js-paper-rank-work_31409668", }); 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34 ؎ 11 years of age). Following an ambulatory control evaluation, all subjects underwent 12 weeks of bed rest. An additional metabolic evaluation was performed after 12 days of reambulation. Bone mineral density declined at the spine (؊2.9%, p ‫؍‬ 0.092) and at the hip (؊3.8%, p ‫؍‬ 0.002 for the trochanter). Bed rest prompted a rapid, sustained, significant increase in urinary calcium and phosphorus as well as a significant increase in serum calcium. Urinary calcium increased from a pre-bed rest value of 5.3 mmol/day to values as high as 7.3 mmol/day during bed rest. Immunoreactive parathyroid hormone and serum 1,25dihydroxyvitamin D declined significantly during bed rest, although the mean values remained within normal limits. Significant changes in bone histology included a suppression of osteoblastic surface for cancellous bone (3.1 ؎ 1.3% to 1.9 ؎ 1.5%, p ‫؍‬ 0.0142) and increased bone resorption for both cancellous and cortical bone. Cortical eroded surface increased from 3.5 ؎ 1.1% to 7.3 ؎ 4.0% (p ‫؍‬ 0.018) as did active osteoclastic surface (0.2 ؎ 0.3% to 0.7 ؎ 0.7%, p ‫؍‬ 0.021). Cancellous eroded surface increased from 2.1 ؎ 1.1% to 4.7 ؎ 2.2% (p ‫؍‬ 0.002), while mean active osteoclastic surface doubled (0.2 ؎ 0.2% to 0.4 ؎ 0.3%, p ‫؍‬ 0.020). Serum biochemical markers of bone formation (osteocalcin, bone-specific alkaline phosphatase, and type I procollagen extension peptide) did not change significantly during bed rest. Urinary biochemical markers of bone resorption (hydroxyproline, deoxypyridinoline, and N-telopeptide of type I collagen) as well as a serum marker of bone resorption (type I collagen carboxytelopeptide) all demonstrated significant increases during bed rest which declined toward normal during reambulation. Thus, under the conditions of this study, the human skeleton appears to respond to unloading by a rapid and sustained increase in bone resorption and a more subtle decrease in bone formation. (J Bone Miner Res 1998;13:1594-1601)","downloadable_attachments":[{"id":51777130,"asset_id":31409668,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34536936,"first_name":"Joseph","last_name":"Zerwekh","domain_name":"independent","page_name":"JosephZerwekh","display_name":"Joseph Zerwekh","profile_url":"https://independent.academia.edu/JosephZerwekh?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=9534","nofollow":true},{"id":9531,"name":"Vitamin D","url":"https://www.academia.edu/Documents/in/Vitamin_D?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true},{"id":88041,"name":"Bone","url":"https://www.academia.edu/Documents/in/Bone?f_ri=9534"},{"id":139532,"name":"Bone Density","url":"https://www.academia.edu/Documents/in/Bone_Density?f_ri=9534"},{"id":288383,"name":"Intestinal absorption","url":"https://www.academia.edu/Documents/in/Intestinal_absorption?f_ri=9534"},{"id":379889,"name":"Homeostasis","url":"https://www.academia.edu/Documents/in/Homeostasis?f_ri=9534"},{"id":438516,"name":"Bone Mineral Density","url":"https://www.academia.edu/Documents/in/Bone_Mineral_Density?f_ri=9534"},{"id":465839,"name":"Bed Rest","url":"https://www.academia.edu/Documents/in/Bed_Rest?f_ri=9534"},{"id":568482,"name":"Biological markers","url":"https://www.academia.edu/Documents/in/Biological_markers?f_ri=9534"},{"id":616689,"name":"Bone remodeling","url":"https://www.academia.edu/Documents/in/Bone_remodeling?f_ri=9534"},{"id":1000427,"name":"Reference Values","url":"https://www.academia.edu/Documents/in/Reference_Values?f_ri=9534"},{"id":1144130,"name":"Weight Bearing","url":"https://www.academia.edu/Documents/in/Weight_Bearing?f_ri=9534"},{"id":1256683,"name":"Bone and Bones","url":"https://www.academia.edu/Documents/in/Bone_and_Bones?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_47127372" data-work_id="47127372" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/47127372/Hidden_Markov_Models_in_Computational_Biology">Hidden Markov Models in Computational Biology</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Ridden .Markov Models (HMMs) are applied t.0 the problems of statistical modeling, database searching and multiple sequence alignment of protein families and protein domains. These methods are demonstrated on the globin family, the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_47127372" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Ridden .Markov Models (HMMs) are applied t.0 the problems of statistical modeling, database searching and multiple sequence alignment of protein families and protein domains. These methods are demonstrated on the globin family, the protein kinase catalytic domain, and the EF-hand calcium binding motif. In each case the parameters of an HMM are estimated from a training set of unaligned sequences. After the HMM is built, it is used to obtain a multiple alignment of all the training sequences. It is also used to search the . SWISS-PROT 22 database for other sequences. that are members of the given protein family, or contain the given domain. The Hi" produces multiple alignments of good quality that agree closely with the alignments produced by programs that incorporate threedimensional structural information. When employed in discrimination tests (by examining how closely the sequences in a database fit the globin, kinase and EF-hand HMMs), the '\ HMM is able to distinguish members of these families from non-members with a high degree ' of accuracy. Both the HMM and PROFILESEARCH ( a technique used to search for relationships between a protein sequence and multiply aligned sequences) perform better in these tests than PROSITE (a dictionary of sites and patterns in proteins). The HMM appecvs to have a slight advantage over PROFILESEARCH in terms of lower rates of false negatives and false positives, even though the HMM is trained using only unaligned sequences, whereas PROFILESEARCH requires aligned training sequences. Our results suggest the presence of an EF-hand calcium binding motif in a highly conserved and evolutionary preserved putative intracellular region of 155 residues in the a-l subunit of L-type calcium channels which play an important role in excitation-contraction coupling. This region has been suggested to contain the functional domains that are typical or essential for all L-type calcium channels regardless of whether they couple to ryanodine receptors, conduct ions or both. \ 8 Abbreviations HMM, hidden Marko,. ,,,dels; length from 130 to 170 residues (with few excep-' m, Expectetion-Maximization; ML. maximum tions) and two domains, the protein kinase catalytic like€ihood; MAP, maximum a posten'wi; hTL-score.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/47127372" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="44a634c54a5785d66b07adef23e1c58c" rel="nofollow" data-download="{"attachment_id":66384909,"asset_id":47127372,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/66384909/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32474099" href="https://independent.academia.edu/KimmenSj%C3%B6lander">Kimmen Sjölander</a><script data-card-contents-for-user="32474099" type="text/json">{"id":32474099,"first_name":"Kimmen","last_name":"Sjölander","domain_name":"independent","page_name":"KimmenSjölander","display_name":"Kimmen Sjölander","profile_url":"https://independent.academia.edu/KimmenSj%C3%B6lander?f_ri=9534","photo":"https://gravatar.com/avatar/922d7f02916642a94880b066f4bab95c?s=65"}</script></span></span></li><li class="js-paper-rank-work_47127372 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="47127372"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 47127372, container: ".js-paper-rank-work_47127372", }); 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These methods are demonstrated on the globin family, the protein kinase catalytic domain, and the EF-hand calcium binding motif. In each case the parameters of an HMM are estimated from a training set of unaligned sequences. After the HMM is built, it is used to obtain a multiple alignment of all the training sequences. It is also used to search the . SWISS-PROT 22 database for other sequences. that are members of the given protein family, or contain the given domain. The Hi\" produces multiple alignments of good quality that agree closely with the alignments produced by programs that incorporate threedimensional structural information. When employed in discrimination tests (by examining how closely the sequences in a database fit the globin, kinase and EF-hand HMMs), the '\\ HMM is able to distinguish members of these families from non-members with a high degree ' of accuracy. Both the HMM and PROFILESEARCH ( a technique used to search for relationships between a protein sequence and multiply aligned sequences) perform better in these tests than PROSITE (a dictionary of sites and patterns in proteins). The HMM appecvs to have a slight advantage over PROFILESEARCH in terms of lower rates of false negatives and false positives, even though the HMM is trained using only unaligned sequences, whereas PROFILESEARCH requires aligned training sequences. Our results suggest the presence of an EF-hand calcium binding motif in a highly conserved and evolutionary preserved putative intracellular region of 155 residues in the a-l subunit of L-type calcium channels which play an important role in excitation-contraction coupling. This region has been suggested to contain the functional domains that are typical or essential for all L-type calcium channels regardless of whether they couple to ryanodine receptors, conduct ions or both. \\ 8 Abbreviations HMM, hidden Marko,. ,,,dels; length from 130 to 170 residues (with few excep-' m, Expectetion-Maximization; ML. maximum tions) and two domains, the protein kinase catalytic like€ihood; MAP, maximum a posten'wi; hTL-score.","downloadable_attachments":[{"id":66384909,"asset_id":47127372,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32474099,"first_name":"Kimmen","last_name":"Sjölander","domain_name":"independent","page_name":"KimmenSjölander","display_name":"Kimmen 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Strength","url":"https://www.academia.edu/Documents/in/Tensile_Strength?f_ri=9534"},{"id":1434995,"name":"Physical and Mechanical Properties","url":"https://www.academia.edu/Documents/in/Physical_and_Mechanical_Properties?f_ri=9534"},{"id":1761489,"name":"Glass Ionomer Cement","url":"https://www.academia.edu/Documents/in/Glass_Ionomer_Cement?f_ri=9534"},{"id":2724508,"name":"Mechanical Property","url":"https://www.academia.edu/Documents/in/Mechanical_Property?f_ri=9534"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_73848242" data-work_id="73848242" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/73848242/Role_of_Extracellular_Polymeric_Substances_EPS_in_Biofouling_of_Reverse_Osmosis_Membranes">Role of Extracellular Polymeric Substances(EPS) in Biofouling of Reverse Osmosis Membranes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Microbially mediated oxidation of Mn(II) to Mn oxides have been demonstrated in previous studies, however, the mechanisms of bacteria how to dissolve and oxidize using a solid Mn(II) origin are poorly understood. In this study, we... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_73848242" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Microbially mediated oxidation of Mn(II) to Mn oxides have been demonstrated in previous studies, however, the mechanisms of bacteria how to dissolve and oxidize using a solid Mn(II) origin are poorly understood. In this study, we examined the role of extracellular polymeric substances (EPS) from P. putida strain MnB1 in enhancing dissolution of natural rhodochrosite. The results showed that P. putida strain MnB1 cell can effectively dissolve and oxidize natural rhodochrosite to generate Mn oxides, and EPS were found to play an important role in increasing dissolution of natural rhodochrosite. Compared with EPS-free treatment, dissolution rate of natural rhodochrosite in the presence of bacterial EPS was significantly increased with decreasing initial pH and increasing EPS concentration, ionic strength and rhodochrosite dosage (p < 0.05). The fourier-transform infrared spectroscopy (FTIR) analysis implies that the functional groups like N-H, C=O and C-H in EPS contributed to the dissolution of natural rhodochrosite. This study is helpful for understanding the mechanisms of the formation of biogenic Mn oxides using a solid Mn(II) origin.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/73848242" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fd303a8e7234f6d23c33f06738ed9e57" rel="nofollow" data-download="{"attachment_id":82218395,"asset_id":73848242,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/82218395/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10646565" href="https://independent.academia.edu/SeoktaeKang">Seoktae Kang</a><script data-card-contents-for-user="10646565" type="text/json">{"id":10646565,"first_name":"Seoktae","last_name":"Kang","domain_name":"independent","page_name":"SeoktaeKang","display_name":"Seoktae Kang","profile_url":"https://independent.academia.edu/SeoktaeKang?f_ri=9534","photo":"https://0.academia-photos.com/10646565/11512688/12840469/s65_seoktae.kang.jpg"}</script></span></span></li><li class="js-paper-rank-work_73848242 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="73848242"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 73848242, container: ".js-paper-rank-work_73848242", }); 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In this study, we examined the role of extracellular polymeric substances (EPS) from P. putida strain MnB1 in enhancing dissolution of natural rhodochrosite. The results showed that P. putida strain MnB1 cell can effectively dissolve and oxidize natural rhodochrosite to generate Mn oxides, and EPS were found to play an important role in increasing dissolution of natural rhodochrosite. Compared with EPS-free treatment, dissolution rate of natural rhodochrosite in the presence of bacterial EPS was significantly increased with decreasing initial pH and increasing EPS concentration, ionic strength and rhodochrosite dosage (p \u003c 0.05). The fourier-transform infrared spectroscopy (FTIR) analysis implies that the functional groups like N-H, C=O and C-H in EPS contributed to the dissolution of natural rhodochrosite. This study is helpful for understanding the mechanisms of the formation of biogenic Mn oxides using a solid Mn(II) origin.","downloadable_attachments":[{"id":82218395,"asset_id":73848242,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":10646565,"first_name":"Seoktae","last_name":"Kang","domain_name":"independent","page_name":"SeoktaeKang","display_name":"Seoktae Kang","profile_url":"https://independent.academia.edu/SeoktaeKang?f_ri=9534","photo":"https://0.academia-photos.com/10646565/11512688/12840469/s65_seoktae.kang.jpg"}],"research_interests":[{"id":2187,"name":"Biofilms","url":"https://www.academia.edu/Documents/in/Biofilms?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":21466,"name":"Polymers","url":"https://www.academia.edu/Documents/in/Polymers?f_ri=9534","nofollow":true},{"id":24373,"name":"Atomic Force Microscopy","url":"https://www.academia.edu/Documents/in/Atomic_Force_Microscopy?f_ri=9534","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=9534"},{"id":28505,"name":"Adhesion","url":"https://www.academia.edu/Documents/in/Adhesion?f_ri=9534"},{"id":39752,"name":"Adsorption","url":"https://www.academia.edu/Documents/in/Adsorption?f_ri=9534"},{"id":63142,"name":"Fouling","url":"https://www.academia.edu/Documents/in/Fouling?f_ri=9534"},{"id":65757,"name":"Wastewater","url":"https://www.academia.edu/Documents/in/Wastewater?f_ri=9534"},{"id":65788,"name":"Biofilm","url":"https://www.academia.edu/Documents/in/Biofilm?f_ri=9534"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=9534"},{"id":130625,"name":"Membrane Separation","url":"https://www.academia.edu/Documents/in/Membrane_Separation?f_ri=9534"},{"id":183724,"name":"Biofouling","url":"https://www.academia.edu/Documents/in/Biofouling?f_ri=9534"},{"id":225499,"name":"Pseudomonas aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_aeruginosa?f_ri=9534"},{"id":276273,"name":"Reverse osmosis","url":"https://www.academia.edu/Documents/in/Reverse_osmosis?f_ri=9534"},{"id":397099,"name":"Biological treatment","url":"https://www.academia.edu/Documents/in/Biological_treatment?f_ri=9534"},{"id":609176,"name":"Osmosis","url":"https://www.academia.edu/Documents/in/Osmosis?f_ri=9534"},{"id":636395,"name":"Pseudomonas Aeruginosa","url":"https://www.academia.edu/Documents/in/Pseudomonas_Aeruginosa-1?f_ri=9534"},{"id":970774,"name":"Ionic Strength","url":"https://www.academia.edu/Documents/in/Ionic_Strength?f_ri=9534"},{"id":1451875,"name":"Calcium Ions","url":"https://www.academia.edu/Documents/in/Calcium_Ions?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_70144097" data-work_id="70144097" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/70144097/Diabetic_Cardiomyopathy_Current_Approach_and_Potential_Diagnostic_and_Therapeutic_Targets">Diabetic Cardiomyopathy: Current Approach and Potential Diagnostic and Therapeutic Targets</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Although ischemic heart disease is the major cause of death in diabetic patients, diabetic cardiomyopathy (DCM) is increasingly recognized as a clinically relevant entity. Considering that it comprises a variety of mechanisms and effects... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_70144097" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Although ischemic heart disease is the major cause of death in diabetic patients, diabetic cardiomyopathy (DCM) is increasingly recognized as a clinically relevant entity. Considering that it comprises a variety of mechanisms and effects on cardiac function, increasing the risk of heart failure and worsening the prognosis of this patient category, DCM represents an important complication of diabetes mellitus, with a silent development in its earlier stages, involving intricate pathophysiological mechanisms, including oxidative stress, defective calcium handling, altered mitochondrial function, remodeling of the extracellular matrix, and consequent deficient cardiomyocyte contractility. While DCM is common in diabetic asymptomatic patients, it is frequently underdiagnosed, due to few diagnostic possibilities in its early stages. Moreover, since a strategy for prevention and treatment in order to improve the prognosis of DCM has not been established, it is important to identify clear ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/70144097" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2bfe6c4daeb696e2a890d69bb99bdb17" rel="nofollow" data-download="{"attachment_id":80000755,"asset_id":70144097,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/80000755/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="48996871" href="https://independent.academia.edu/IrisBararu">Iris Bararu</a><script data-card-contents-for-user="48996871" type="text/json">{"id":48996871,"first_name":"Iris","last_name":"Bararu","domain_name":"independent","page_name":"IrisBararu","display_name":"Iris Bararu","profile_url":"https://independent.academia.edu/IrisBararu?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_70144097 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="70144097"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 70144097, container: ".js-paper-rank-work_70144097", }); 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Considering that it comprises a variety of mechanisms and effects on cardiac function, increasing the risk of heart failure and worsening the prognosis of this patient category, DCM represents an important complication of diabetes mellitus, with a silent development in its earlier stages, involving intricate pathophysiological mechanisms, including oxidative stress, defective calcium handling, altered mitochondrial function, remodeling of the extracellular matrix, and consequent deficient cardiomyocyte contractility. While DCM is common in diabetic asymptomatic patients, it is frequently underdiagnosed, due to few diagnostic possibilities in its early stages. Moreover, since a strategy for prevention and treatment in order to improve the prognosis of DCM has not been established, it is important to identify clear ...","downloadable_attachments":[{"id":80000755,"asset_id":70144097,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":48996871,"first_name":"Iris","last_name":"Bararu","domain_name":"independent","page_name":"IrisBararu","display_name":"Iris Bararu","profile_url":"https://independent.academia.edu/IrisBararu?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4581,"name":"Diabetes","url":"https://www.academia.edu/Documents/in/Diabetes?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":21732,"name":"Magnetic Resonance Spectroscopy","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Spectroscopy?f_ri=9534","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=9534","nofollow":true},{"id":198747,"name":"Left Ventricular Dysfunction","url":"https://www.academia.edu/Documents/in/Left_Ventricular_Dysfunction?f_ri=9534"},{"id":295854,"name":"microRNAs","url":"https://www.academia.edu/Documents/in/microRNAs?f_ri=9534"},{"id":1146831,"name":"Exercise Test","url":"https://www.academia.edu/Documents/in/Exercise_Test?f_ri=9534"},{"id":1212105,"name":"Molecular Targeted Therapy","url":"https://www.academia.edu/Documents/in/Molecular_Targeted_Therapy?f_ri=9534"},{"id":1969247,"name":"Ubiquitination","url":"https://www.academia.edu/Documents/in/Ubiquitination?f_ri=9534"},{"id":3221667,"name":"Diastole","url":"https://www.academia.edu/Documents/in/Diastole?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_70088582" data-work_id="70088582" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" href="https://www.academia.edu/70088582/Intracellular_signaling_cascades_induced_by_relaxin_in_the_stimulation_of_capacitation_and_acrosome_reaction_in_fresh_and_frozen_thawed_bovine_spermatozoa">Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_70088582" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in fresh and frozen-thawed porcine or bovine spermatozoa. However, the intracellular signaling cascades involved with capacitation or AR induced by relaxin was unknown. Therefore, the present study was designed to investigate the intracellular signaling cascades involved with capacitation and AR induced by relaxin in fresh and frozen-thawed bovine spermatozoa. Spermatozoa were incubated in sperm Tyrode&amp;#39;s albumin lactate pyruvate (Sp-TALP) medium supplemented with (40 ng ml(-1)) or without relaxin, and subjected to evaluation of chlortetracycline staining pattern, cholesterol efflux, Ca(2+)-influx, intracellular cyclic adenosine monophosphate (cAMP) and protein tyrosine phosphorylation. Capacitation and AR were increased (P&amp;lt;0.05) in both fresh and frozen-thawed spermatozoa incubated with relaxin. Cholesterol effluxes were greater in the fresh (P&amp;lt;0.01) and frozen-thawed (P&amp;lt;0.05) spermatozoa incubated with relaxin than the spermatozoa incubated without relaxin. Ca(2+)-influxes were also significantly stimulated by relaxin in the fresh (P&amp;lt;0.01) and frozen-thawed (P&amp;lt;0.05) spermatozoa. The Sp-TALP medium containing relaxin influenced the generation of intracellular cAMP in the fresh (P&amp;lt;0.01) and frozen-thawed (P&amp;lt;0.05) spermatozoa, and exhibited higher exposure of protein tyrosine phosphorylation in both sperm types than the medium devoid of relaxin. Therefore, the results postulate that relaxin exerts the intracellular signaling cascades involved with capacitation and AR through accelerating the cholesterol efflux, Ca(2+)-influx, intracellular cAMP and protein tyrosine phosphorylation in fresh and frozen-thawed bovine spermatozoa.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/70088582" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="13186152" href="https://independent.academia.edu/AbdulGaffarMiah">Abdul Gaffar Miah</a><script data-card-contents-for-user="13186152" type="text/json">{"id":13186152,"first_name":"Abdul Gaffar","last_name":"Miah","domain_name":"independent","page_name":"AbdulGaffarMiah","display_name":"Abdul Gaffar Miah","profile_url":"https://independent.academia.edu/AbdulGaffarMiah?f_ri=9534","photo":"https://0.academia-photos.com/13186152/175731379/165776970/s65_abdul_gaffar.miah.png"}</script></span></span></li><li class="js-paper-rank-work_70088582 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="70088582"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 70088582, container: ".js-paper-rank-work_70088582", }); 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$(".js-view-count[data-work-id=70088582]").text(description); $(".js-view-count-work_70088582").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_70088582").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="70088582"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">17</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="7700" rel="nofollow" href="https://www.academia.edu/Documents/in/Fluorescence_Microscopy">Fluorescence Microscopy</a>, <script data-card-contents-for-ri="7700" type="text/json">{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="18613" rel="nofollow" href="https://www.academia.edu/Documents/in/Animal_reproduction">Animal reproduction</a>, <script data-card-contents-for-ri="18613" type="text/json">{"id":18613,"name":"Animal reproduction","url":"https://www.academia.edu/Documents/in/Animal_reproduction?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="38831" rel="nofollow" href="https://www.academia.edu/Documents/in/Signal_Transduction">Signal Transduction</a><script data-card-contents-for-ri="38831" type="text/json">{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=70088582]'), work: {"id":70088582,"title":"Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa","created_at":"2022-01-30T19:05:45.796-08:00","url":"https://www.academia.edu/70088582/Intracellular_signaling_cascades_induced_by_relaxin_in_the_stimulation_of_capacitation_and_acrosome_reaction_in_fresh_and_frozen_thawed_bovine_spermatozoa?f_ri=9534","dom_id":"work_70088582","summary":"Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in fresh and frozen-thawed porcine or bovine spermatozoa. However, the intracellular signaling cascades involved with capacitation or AR induced by relaxin was unknown. Therefore, the present study was designed to investigate the intracellular signaling cascades involved with capacitation and AR induced by relaxin in fresh and frozen-thawed bovine spermatozoa. Spermatozoa were incubated in sperm Tyrode\u0026amp;#39;s albumin lactate pyruvate (Sp-TALP) medium supplemented with (40 ng ml(-1)) or without relaxin, and subjected to evaluation of chlortetracycline staining pattern, cholesterol efflux, Ca(2+)-influx, intracellular cyclic adenosine monophosphate (cAMP) and protein tyrosine phosphorylation. Capacitation and AR were increased (P\u0026amp;lt;0.05) in both fresh and frozen-thawed spermatozoa incubated with relaxin. Cholesterol effluxes were greater in the fresh (P\u0026amp;lt;0.01) and frozen-thawed (P\u0026amp;lt;0.05) spermatozoa incubated with relaxin than the spermatozoa incubated without relaxin. Ca(2+)-influxes were also significantly stimulated by relaxin in the fresh (P\u0026amp;lt;0.01) and frozen-thawed (P\u0026amp;lt;0.05) spermatozoa. The Sp-TALP medium containing relaxin influenced the generation of intracellular cAMP in the fresh (P\u0026amp;lt;0.01) and frozen-thawed (P\u0026amp;lt;0.05) spermatozoa, and exhibited higher exposure of protein tyrosine phosphorylation in both sperm types than the medium devoid of relaxin. Therefore, the results postulate that relaxin exerts the intracellular signaling cascades involved with capacitation and AR through accelerating the cholesterol efflux, Ca(2+)-influx, intracellular cAMP and protein tyrosine phosphorylation in fresh and frozen-thawed bovine spermatozoa.","downloadable_attachments":[],"ordered_authors":[{"id":13186152,"first_name":"Abdul Gaffar","last_name":"Miah","domain_name":"independent","page_name":"AbdulGaffarMiah","display_name":"Abdul Gaffar Miah","profile_url":"https://independent.academia.edu/AbdulGaffarMiah?f_ri=9534","photo":"https://0.academia-photos.com/13186152/175731379/165776970/s65_abdul_gaffar.miah.png"}],"research_interests":[{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":18613,"name":"Animal reproduction","url":"https://www.academia.edu/Documents/in/Animal_reproduction?f_ri=9534","nofollow":true},{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction?f_ri=9534","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534"},{"id":90514,"name":"Cholesterol","url":"https://www.academia.edu/Documents/in/Cholesterol?f_ri=9534"},{"id":92680,"name":"cyclic AMP","url":"https://www.academia.edu/Documents/in/cyclic_AMP?f_ri=9534"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=9534"},{"id":846581,"name":"Cell communication","url":"https://www.academia.edu/Documents/in/Cell_communication?f_ri=9534"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=9534"},{"id":1048679,"name":"Chlortetracycline","url":"https://www.academia.edu/Documents/in/Chlortetracycline?f_ri=9534"},{"id":1212380,"name":"Intracellular Signaling","url":"https://www.academia.edu/Documents/in/Intracellular_Signaling?f_ri=9534"},{"id":1219924,"name":"Acrosome Reaction","url":"https://www.academia.edu/Documents/in/Acrosome_Reaction?f_ri=9534"},{"id":1953128,"name":"Tyrosine Phosphorylation","url":"https://www.academia.edu/Documents/in/Tyrosine_Phosphorylation?f_ri=9534"},{"id":2156592,"name":"Relaxin","url":"https://www.academia.edu/Documents/in/Relaxin?f_ri=9534"},{"id":2812233,"name":"Sperm capacitation","url":"https://www.academia.edu/Documents/in/Sperm_capacitation?f_ri=9534"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62905440" data-work_id="62905440" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62905440/Hydrogen_peroxide_as_an_alternate_substrate_for_the_oxygen_evolving_complex">Hydrogen peroxide as an alternate substrate for the oxygen-evolving complex</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Photosystem II reaction centers evolve 02 in the dark when H 202 is added as a substrate. Although some of this activity can be attributed to catalase, as much as 75% of the activity was not affected by the addition of 1 mM KCN. Several... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_62905440" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Photosystem II reaction centers evolve 02 in the dark when H 202 is added as a substrate. Although some of this activity can be attributed to catalase, as much as 75% of the activity was not affected by the addition of 1 mM KCN. Several lines of evidence demonstrate that this KCN-insensitive 02 evolution from H 202 in the dark is catalyzed by the cycling of S states in the oxygen-evolving complex including: (1) inactivation of H2Oz-mediated 02 evolution by Ca/EDTA washing; (2) susceptibility of the activity to inhibition by amines like ammonia and Tris; (3) inhibition by CCCP which is known to accelerate the rate of deactivation of the S 2 state and; (4) a direct dependence of the rate of 02 evolution on the presence of calcium and (5) chloride.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62905440" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7e43b3ee9db0aedbe9a965143b73684d" rel="nofollow" data-download="{"attachment_id":75519009,"asset_id":62905440,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75519009/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="44252059" href="https://independent.academia.edu/RuiMei">Rui Mei</a><script data-card-contents-for-user="44252059" type="text/json">{"id":44252059,"first_name":"Rui","last_name":"Mei","domain_name":"independent","page_name":"RuiMei","display_name":"Rui Mei","profile_url":"https://independent.academia.edu/RuiMei?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_62905440 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="62905440"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 62905440, container: ".js-paper-rank-work_62905440", }); 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Although some of this activity can be attributed to catalase, as much as 75% of the activity was not affected by the addition of 1 mM KCN. Several lines of evidence demonstrate that this KCN-insensitive 02 evolution from H 202 in the dark is catalyzed by the cycling of S states in the oxygen-evolving complex including: (1) inactivation of H2Oz-mediated 02 evolution by Ca/EDTA washing; (2) susceptibility of the activity to inhibition by amines like ammonia and Tris; (3) inhibition by CCCP which is known to accelerate the rate of deactivation of the S 2 state and; (4) a direct dependence of the rate of 02 evolution on the presence of calcium and (5) chloride.","downloadable_attachments":[{"id":75519009,"asset_id":62905440,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44252059,"first_name":"Rui","last_name":"Mei","domain_name":"independent","page_name":"RuiMei","display_name":"Rui Mei","profile_url":"https://independent.academia.edu/RuiMei?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":29057,"name":"Manganese","url":"https://www.academia.edu/Documents/in/Manganese?f_ri=9534","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true},{"id":85564,"name":"Chlorophyll","url":"https://www.academia.edu/Documents/in/Chlorophyll?f_ri=9534","nofollow":true},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=9534"},{"id":274826,"name":"Hydrogen Peroxide","url":"https://www.academia.edu/Documents/in/Hydrogen_Peroxide?f_ri=9534"},{"id":380825,"name":"Oxygen","url":"https://www.academia.edu/Documents/in/Oxygen?f_ri=9534"},{"id":669339,"name":"Mes","url":"https://www.academia.edu/Documents/in/Mes?f_ri=9534"},{"id":782600,"name":"Oxygen Evolving Complex","url":"https://www.academia.edu/Documents/in/Oxygen_Evolving_Complex?f_ri=9534"},{"id":849801,"name":"Photosystem II","url":"https://www.academia.edu/Documents/in/Photosystem_II?f_ri=9534"},{"id":1181939,"name":"PLANT PROTEINS","url":"https://www.academia.edu/Documents/in/PLANT_PROTEINS?f_ri=9534"},{"id":1451595,"name":"Edetic Acid","url":"https://www.academia.edu/Documents/in/Edetic_Acid?f_ri=9534"},{"id":1553580,"name":"Oxygen evolution reaction","url":"https://www.academia.edu/Documents/in/Oxygen_evolution_reaction?f_ri=9534"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=9534"},{"id":2260515,"name":"Chlorides","url":"https://www.academia.edu/Documents/in/Chlorides?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_60822307" data-work_id="60822307" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/60822307/Changes_in_bone_mineral_density_and_markers_of_bone_remodeling_during_lactation_and_postweaning_in_women_consuming_high_amounts_of_calcium">Changes in bone mineral density and markers of bone remodeling during lactation and postweaning in women consuming high amounts of calcium</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A randomized clinical intervention trial to determine effects of lactation and 1 g of calcium (Ca) on bone remodeling was conducted in 15 women (calcium = 7, placebo [PI = 8) consuming 13-2.4 g of Ca/day from diet + prenatal supplement.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_60822307" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A randomized clinical intervention trial to determine effects of lactation and 1 g of calcium (Ca) on bone remodeling was conducted in 15 women (calcium = 7, placebo [PI = 8) consuming 13-2.4 g of Ca/day from diet + prenatal supplement. Study periods were baseline, 5 2 weeks postpartum; lactation, 3 months lactation; and postweaning, 3 months postweaning. Bone mineral density (BMD) corrected for body weight was determined by dual-energy X-ray absorptiometry (DXA). Indicators of calcium metabolism, bone turnover, and lactation were measured: calcium metabolism, parathyroid hormone (PTH), 25-hydroxyvitamin D (25 [OH] D), 1,25-dihydroxyvitamin D (1,25 [OH],D); bone turnover, formation, procollagen I carboxypeptides (PICP), osteocalcin, and bone alkaline phosphatase (B-ALP), resorption, tartrate resistant acid phosphatase (TRAP); and lactation, prolactin (PRL). Mean BMD changes differed by site: baseline to lactation-43% (P) (p < 0.04) and-63% (Ca) (p < 0.01) at the lumbar spine (L2-U) and 5.7% gains of the ultradistal (UD) radius (Ca) (p < 0.04); lactation to postweaning,-6% to-11% at all sites of the radius and ulna (Ca, P) (p < 0.04) +3% at L2-U (Ca) (p < 0.03); baseline to postweaning, (UD) radius-5.2% (P) (p < 0.03), UD radius + ulna-6% to-8% (Ca, P) (p < 0.04) but no significant loss of L2-L4 or total body. Bone turnover markers were higher at lactation than postweaning: PICP (+34%,p < 0.001), osteocalcin (+25%,p < 0.01), TRAP (+ll%,p < 0.005) as was PRL (+81%,p < 0.001). Indicators of calcium metabolism were higher postweaning than lactation for PTH (+4wo,p < 0.01) and 25(OH)D (+45%, p < 0.02) but not for 1,25(OH),D. There were no differences between P or Ca for indices of calcium metabolism, bone turnover, or PRL. An increase in markers of bone turnover and a loss of BMD of the spine during lactation appears to be part of the physiological changes of lactation and not preventable by increasing calcium intake above the recommended dietary allowance (RDA). A return of BMD toward baseline of the spine but not the arm, was associated with an increase in PTH without an increase in 1,25(OH),D postweaning. Loss of estrogen during lactation and a return postweaning may play an important role.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/60822307" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fc08d65431bab9c2e7862ecff9d86626" rel="nofollow" data-download="{"attachment_id":74096345,"asset_id":60822307,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/74096345/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="13659628" href="https://independent.academia.edu/NannaCross">Nanna Cross</a><script data-card-contents-for-user="13659628" type="text/json">{"id":13659628,"first_name":"Nanna","last_name":"Cross","domain_name":"independent","page_name":"NannaCross","display_name":"Nanna Cross","profile_url":"https://independent.academia.edu/NannaCross?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_60822307 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="60822307"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 60822307, container: ".js-paper-rank-work_60822307", }); 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Study periods were baseline, 5 2 weeks postpartum; lactation, 3 months lactation; and postweaning, 3 months postweaning. Bone mineral density (BMD) corrected for body weight was determined by dual-energy X-ray absorptiometry (DXA). Indicators of calcium metabolism, bone turnover, and lactation were measured: calcium metabolism, parathyroid hormone (PTH), 25-hydroxyvitamin D (25 [OH] D), 1,25-dihydroxyvitamin D (1,25 [OH],D); bone turnover, formation, procollagen I carboxypeptides (PICP), osteocalcin, and bone alkaline phosphatase (B-ALP), resorption, tartrate resistant acid phosphatase (TRAP); and lactation, prolactin (PRL). Mean BMD changes differed by site: baseline to lactation-43% (P) (p \u003c 0.04) and-63% (Ca) (p \u003c 0.01) at the lumbar spine (L2-U) and 5.7% gains of the ultradistal (UD) radius (Ca) (p \u003c 0.04); lactation to postweaning,-6% to-11% at all sites of the radius and ulna (Ca, P) (p \u003c 0.04) +3% at L2-U (Ca) (p \u003c 0.03); baseline to postweaning, (UD) radius-5.2% (P) (p \u003c 0.03), UD radius + ulna-6% to-8% (Ca, P) (p \u003c 0.04) but no significant loss of L2-L4 or total body. Bone turnover markers were higher at lactation than postweaning: PICP (+34%,p \u003c 0.001), osteocalcin (+25%,p \u003c 0.01), TRAP (+ll%,p \u003c 0.005) as was PRL (+81%,p \u003c 0.001). Indicators of calcium metabolism were higher postweaning than lactation for PTH (+4wo,p \u003c 0.01) and 25(OH)D (+45%, p \u003c 0.02) but not for 1,25(OH),D. There were no differences between P or Ca for indices of calcium metabolism, bone turnover, or PRL. An increase in markers of bone turnover and a loss of BMD of the spine during lactation appears to be part of the physiological changes of lactation and not preventable by increasing calcium intake above the recommended dietary allowance (RDA). A return of BMD toward baseline of the spine but not the arm, was associated with an increase in PTH without an increase in 1,25(OH),D postweaning. Loss of estrogen during lactation and a return postweaning may play an important role.","downloadable_attachments":[{"id":74096345,"asset_id":60822307,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":13659628,"first_name":"Nanna","last_name":"Cross","domain_name":"independent","page_name":"NannaCross","display_name":"Nanna Cross","profile_url":"https://independent.academia.edu/NannaCross?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=9534","nofollow":true},{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":17726,"name":"Density","url":"https://www.academia.edu/Documents/in/Density?f_ri=9534","nofollow":true},{"id":19826,"name":"Lactation","url":"https://www.academia.edu/Documents/in/Lactation?f_ri=9534"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534"},{"id":88041,"name":"Bone","url":"https://www.academia.edu/Documents/in/Bone?f_ri=9534"},{"id":109951,"name":"Turnover","url":"https://www.academia.edu/Documents/in/Turnover?f_ri=9534"},{"id":139532,"name":"Bone Density","url":"https://www.academia.edu/Documents/in/Bone_Density?f_ri=9534"},{"id":170690,"name":"Weaning","url":"https://www.academia.edu/Documents/in/Weaning?f_ri=9534"},{"id":192551,"name":"Nutritional Status","url":"https://www.academia.edu/Documents/in/Nutritional_Status?f_ri=9534"},{"id":379889,"name":"Homeostasis","url":"https://www.academia.edu/Documents/in/Homeostasis?f_ri=9534"},{"id":413194,"name":"Analysis of Variance","url":"https://www.academia.edu/Documents/in/Analysis_of_Variance?f_ri=9534"},{"id":438516,"name":"Bone Mineral Density","url":"https://www.academia.edu/Documents/in/Bone_Mineral_Density?f_ri=9534"},{"id":568482,"name":"Biological markers","url":"https://www.academia.edu/Documents/in/Biological_markers?f_ri=9534"},{"id":616689,"name":"Bone remodeling","url":"https://www.academia.edu/Documents/in/Bone_remodeling?f_ri=9534"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_60531310" data-work_id="60531310" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" rel="nofollow" href="https://www.academia.edu/60531310/Rabbit_dental_disease_and_calcium_metabolism_the_science_behind_divided_opinions">Rabbit dental disease and calcium metabolism – the science behind divided opinions</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Dental disease is considered as one of the, if not, the most common disorders seen in pet rabbits. This article provides a review of the scientific literature and an overview of the peculiarities of calcium homeostasis in the rabbit in an... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_60531310" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Dental disease is considered as one of the, if not, the most common disorders seen in pet rabbits. This article provides a review of the scientific literature and an overview of the peculiarities of calcium homeostasis in the rabbit in an attempt to draw together current thinking on the cause of dental disease. A complete understanding of the aetiology and pathophysiology of rabbit dental disease is necessary for the veterinary practitioner to establish a proper therapeutic plan, prognosis and ultimately prevention of this common cause of morbidity and mortality in pet rabbits.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/60531310" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41263051" href="https://nottingham.academia.edu/SharonRedrobe">Sharon Redrobe</a><script data-card-contents-for-user="41263051" type="text/json">{"id":41263051,"first_name":"Sharon","last_name":"Redrobe","domain_name":"nottingham","page_name":"SharonRedrobe","display_name":"Sharon Redrobe","profile_url":"https://nottingham.academia.edu/SharonRedrobe?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_60531310 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="60531310"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 60531310, container: ".js-paper-rank-work_60531310", }); 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This article provides a review of the scientific literature and an overview of the peculiarities of calcium homeostasis in the rabbit in an attempt to draw together current thinking on the cause of dental disease. A complete understanding of the aetiology and pathophysiology of rabbit dental disease is necessary for the veterinary practitioner to establish a proper therapeutic plan, prognosis and ultimately prevention of this common cause of morbidity and mortality in pet rabbits.","downloadable_attachments":[],"ordered_authors":[{"id":41263051,"first_name":"Sharon","last_name":"Redrobe","domain_name":"nottingham","page_name":"SharonRedrobe","display_name":"Sharon Redrobe","profile_url":"https://nottingham.academia.edu/SharonRedrobe?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":59869,"name":"Metabolic Bone Disease","url":"https://www.academia.edu/Documents/in/Metabolic_Bone_Disease?f_ri=9534","nofollow":true},{"id":120118,"name":"Malocclusion","url":"https://www.academia.edu/Documents/in/Malocclusion?f_ri=9534","nofollow":true},{"id":179071,"name":"Rabbit","url":"https://www.academia.edu/Documents/in/Rabbit?f_ri=9534","nofollow":true},{"id":394570,"name":"Dental Disease","url":"https://www.academia.edu/Documents/in/Dental_Disease?f_ri=9534"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences?f_ri=9534"},{"id":721269,"name":"Small Animal Practice","url":"https://www.academia.edu/Documents/in/Small_Animal_Practice?f_ri=9534"},{"id":1088744,"name":"Hypocalcaemia","url":"https://www.academia.edu/Documents/in/Hypocalcaemia?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_54131140" data-work_id="54131140" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/54131140/Dissociation_of_Limulus_polyphemus_horseshoe_crab_hemocyanin">Dissociation of Limulus polyphemus (horseshoe crab) hemocyanin</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A solution X-ray scattering study has been performed on Limulus polyphemus (horseshoe crab) hemocyanin and its dissociated fragments at various pH values in the presence and absence of Ca *'. The scattering patterns of native hemocyanin... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_54131140" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A solution X-ray scattering study has been performed on Limulus polyphemus (horseshoe crab) hemocyanin and its dissociated fragments at various pH values in the presence and absence of Ca *'. The scattering patterns of native hemocyanin (4%mer), the half molecule (24mer), quarter molecule (Ibmer) and monomer fraction were measured. The radii of gyration for the four molecular species were calculated from the Guinier plots to be 110.7, 91.3, 77.3, and 36.5 A, respectively. Models which yield good fits to the experimental data are presented. The models were constructed using eight, four and two spheres with a radius of 58 A, assuming the sphere to be the submultiple composed of six subunits. The radir of gyration were calculated on the basis of the model and the values found to be 106, 94 and 73 A, respectively, in good agreement with the experimental results.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/54131140" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="44c4b72dc05b328f6ae51c5775990664" rel="nofollow" data-download="{"attachment_id":70644504,"asset_id":54131140,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/70644504/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="53217206" href="https://independent.academia.edu/HiroshiKihara">Hiroshi Kihara</a><script data-card-contents-for-user="53217206" type="text/json">{"id":53217206,"first_name":"Hiroshi","last_name":"Kihara","domain_name":"independent","page_name":"HiroshiKihara","display_name":"Hiroshi Kihara","profile_url":"https://independent.academia.edu/HiroshiKihara?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_54131140 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="54131140"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 54131140, container: ".js-paper-rank-work_54131140", }); 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$(".js-view-count[data-work-id=54131140]").text(description); $(".js-view-count-work_54131140").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_54131140").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="54131140"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="10636" rel="nofollow" href="https://www.academia.edu/Documents/in/Small_Angle_X_Ray_Scattering">Small Angle X Ray Scattering</a>, <script data-card-contents-for-ri="10636" type="text/json">{"id":10636,"name":"Small Angle X Ray Scattering","url":"https://www.academia.edu/Documents/in/Small_Angle_X_Ray_Scattering?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="19156" rel="nofollow" href="https://www.academia.edu/Documents/in/Biophysical_Chemistry">Biophysical Chemistry</a>, <script data-card-contents-for-ri="19156" type="text/json">{"id":19156,"name":"Biophysical Chemistry","url":"https://www.academia.edu/Documents/in/Biophysical_Chemistry?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a><script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=54131140]'), work: {"id":54131140,"title":"Dissociation of Limulus polyphemus (horseshoe crab) hemocyanin","created_at":"2021-09-29T22:11:30.984-07:00","url":"https://www.academia.edu/54131140/Dissociation_of_Limulus_polyphemus_horseshoe_crab_hemocyanin?f_ri=9534","dom_id":"work_54131140","summary":"A solution X-ray scattering study has been performed on Limulus polyphemus (horseshoe crab) hemocyanin and its dissociated fragments at various pH values in the presence and absence of Ca *'. The scattering patterns of native hemocyanin (4%mer), the half molecule (24mer), quarter molecule (Ibmer) and monomer fraction were measured. The radii of gyration for the four molecular species were calculated from the Guinier plots to be 110.7, 91.3, 77.3, and 36.5 A, respectively. Models which yield good fits to the experimental data are presented. The models were constructed using eight, four and two spheres with a radius of 58 A, assuming the sphere to be the submultiple composed of six subunits. The radir of gyration were calculated on the basis of the model and the values found to be 106, 94 and 73 A, respectively, in good agreement with the experimental results.","downloadable_attachments":[{"id":70644504,"asset_id":54131140,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":53217206,"first_name":"Hiroshi","last_name":"Kihara","domain_name":"independent","page_name":"HiroshiKihara","display_name":"Hiroshi Kihara","profile_url":"https://independent.academia.edu/HiroshiKihara?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":10636,"name":"Small Angle X Ray Scattering","url":"https://www.academia.edu/Documents/in/Small_Angle_X_Ray_Scattering?f_ri=9534","nofollow":true},{"id":19156,"name":"Biophysical Chemistry","url":"https://www.academia.edu/Documents/in/Biophysical_Chemistry?f_ri=9534","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true},{"id":51757,"name":"Dissociation","url":"https://www.academia.edu/Documents/in/Dissociation?f_ri=9534"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=9534"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=9534"},{"id":463300,"name":"Molecular Model","url":"https://www.academia.edu/Documents/in/Molecular_Model?f_ri=9534"},{"id":664552,"name":"Radius of Gyration","url":"https://www.academia.edu/Documents/in/Radius_of_Gyration?f_ri=9534"},{"id":1354264,"name":"Subunit","url":"https://www.academia.edu/Documents/in/Subunit?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_53158243" data-work_id="53158243" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/53158243/Experienced_radio_guided_surgery_teams_can_successfully_perform_minimally_invasive_radio_guided_parathyroidectomy_without_intraoperative_parathyroid_hormone_">Experienced radio-guided surgery teams can successfully perform minimally invasive radio-guided parathyroidectomy without intraoperative parathyroid hormone …</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Abstract: Minimally invasive parathyroidectomy is an accepted treatment option for primary hyperparathyroidism. The need for intraoperative parathyroid hormone assays (iPTH) to confirm adenoma removal remains controversial. We studied... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_53158243" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Abstract: Minimally invasive parathyroidectomy is an accepted treatment option for primary hyperparathyroidism. The need for intraoperative parathyroid hormone assays (iPTH) to confirm adenoma removal remains controversial. We studied minimally invasive radio-...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/53158243" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="68c20ac823880fc88bc8b7ffcb18f7f8" rel="nofollow" data-download="{"attachment_id":70077271,"asset_id":53158243,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/70077271/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="36500216" href="https://independent.academia.edu/BenjaminCalvo">Benjamin Calvo</a><script data-card-contents-for-user="36500216" type="text/json">{"id":36500216,"first_name":"Benjamin","last_name":"Calvo","domain_name":"independent","page_name":"BenjaminCalvo","display_name":"Benjamin Calvo","profile_url":"https://independent.academia.edu/BenjaminCalvo?f_ri=9534","photo":"https://0.academia-photos.com/36500216/19174675/19120643/s65_benjamin.calvo.jpg"}</script></span></span></li><li class="js-paper-rank-work_53158243 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="53158243"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 53158243, container: ".js-paper-rank-work_53158243", }); });</script></li><li class="js-percentile-work_53158243 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 53158243; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_53158243"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_53158243 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="53158243"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53158243; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=53158243]").text(description); $(".js-view-count-work_53158243").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_53158243").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="53158243"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="7986" rel="nofollow" href="https://www.academia.edu/Documents/in/Radiosurgery">Radiosurgery</a>, <script data-card-contents-for-ri="7986" type="text/json">{"id":7986,"name":"Radiosurgery","url":"https://www.academia.edu/Documents/in/Radiosurgery?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" rel="nofollow" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a>, <script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="203383" rel="nofollow" href="https://www.academia.edu/Documents/in/Parathyroid_Hormone">Parathyroid Hormone</a><script data-card-contents-for-ri="203383" type="text/json">{"id":203383,"name":"Parathyroid Hormone","url":"https://www.academia.edu/Documents/in/Parathyroid_Hormone?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=53158243]'), work: {"id":53158243,"title":"Experienced radio-guided surgery teams can successfully perform minimally invasive radio-guided parathyroidectomy without intraoperative parathyroid hormone …","created_at":"2021-09-21T13:41:50.177-07:00","url":"https://www.academia.edu/53158243/Experienced_radio_guided_surgery_teams_can_successfully_perform_minimally_invasive_radio_guided_parathyroidectomy_without_intraoperative_parathyroid_hormone_?f_ri=9534","dom_id":"work_53158243","summary":"Abstract: Minimally invasive parathyroidectomy is an accepted treatment option for primary hyperparathyroidism. The need for intraoperative parathyroid hormone assays (iPTH) to confirm adenoma removal remains controversial. We studied minimally invasive radio-...","downloadable_attachments":[{"id":70077271,"asset_id":53158243,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36500216,"first_name":"Benjamin","last_name":"Calvo","domain_name":"independent","page_name":"BenjaminCalvo","display_name":"Benjamin Calvo","profile_url":"https://independent.academia.edu/BenjaminCalvo?f_ri=9534","photo":"https://0.academia-photos.com/36500216/19174675/19120643/s65_benjamin.calvo.jpg"}],"research_interests":[{"id":7986,"name":"Radiosurgery","url":"https://www.academia.edu/Documents/in/Radiosurgery?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=9534","nofollow":true},{"id":203383,"name":"Parathyroid Hormone","url":"https://www.academia.edu/Documents/in/Parathyroid_Hormone?f_ri=9534","nofollow":true},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=9534"},{"id":469105,"name":"Retrospective Studies","url":"https://www.academia.edu/Documents/in/Retrospective_Studies?f_ri=9534"},{"id":704648,"name":"Clinical Competence","url":"https://www.academia.edu/Documents/in/Clinical_Competence?f_ri=9534"},{"id":963748,"name":"Adenoma","url":"https://www.academia.edu/Documents/in/Adenoma?f_ri=9534"},{"id":1399312,"name":"Intraoperative Neurophysiological Monitoring","url":"https://www.academia.edu/Documents/in/Intraoperative_Neurophysiological_Monitoring?f_ri=9534"},{"id":1863718,"name":"The American","url":"https://www.academia.edu/Documents/in/The_American?f_ri=9534"},{"id":2201015,"name":"Postoperative care","url":"https://www.academia.edu/Documents/in/Postoperative_care?f_ri=9534"},{"id":2487845,"name":"Parathyroidectomy","url":"https://www.academia.edu/Documents/in/Parathyroidectomy?f_ri=9534"},{"id":4022629,"name":"minimally invasive surgical procedures","url":"https://www.academia.edu/Documents/in/minimally_invasive_surgical_procedures?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_50467502" data-work_id="50467502" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/50467502/Differential_effects_of_mercurial_compounds_on_excitable_tissues">Differential effects of mercurial compounds on excitable tissues</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Sarcoplasmic reticulum (SR), Ca *+ + Mg *+-ATPase, and Ca *+-ioncphore were obtained from white rabbit skeletal muscles. Methylmercury inhibited the Ca *+ + Mg*+-ATPase an d Ca**-transport but had no effect on the Ca*+ionophore. Mercuric... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_50467502" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Sarcoplasmic reticulum (SR), Ca *+ + Mg *+-ATPase, and Ca *+-ioncphore were obtained from white rabbit skeletal muscles. Methylmercury inhibited the Ca *+ + Mg*+-ATPase an d Ca**-transport but had no effect on the Ca*+ionophore. Mercuric chloride inhibited all three functions (i.e., ATPase, transport and ionophoric activity). The mechanism of HgC12 inhibition of the Ca*+ionophore was by competition with Ca*+ for Ca*'-ionophoric site whereas its inhibition of the enzyme ar,d Ca*+-transport was due to the blockage of essential sulfhydryl (-SH) groups. Ca*' + Mg*+-ATPase and Ca*+-transport were more sensitive to methylmercury than to HgC12. Acetylcholine receptor (AChR) was obtained for the electric organ of T.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/50467502" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2d05cc17b2660497c95b5874040f97a8" rel="nofollow" data-download="{"attachment_id":68443266,"asset_id":50467502,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/68443266/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46718799" href="https://independent.academia.edu/AdilShamoo">Adil Shamoo</a><script data-card-contents-for-user="46718799" type="text/json">{"id":46718799,"first_name":"Adil","last_name":"Shamoo","domain_name":"independent","page_name":"AdilShamoo","display_name":"Adil Shamoo","profile_url":"https://independent.academia.edu/AdilShamoo?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_50467502 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="50467502"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 50467502, container: ".js-paper-rank-work_50467502", }); });</script></li><li class="js-percentile-work_50467502 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50467502; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_50467502"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_50467502 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="50467502"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50467502; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50467502]").text(description); $(".js-view-count-work_50467502").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_50467502").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="50467502"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2513" rel="nofollow" href="https://www.academia.edu/Documents/in/Molecular_Biology">Molecular Biology</a>, <script data-card-contents-for-ri="2513" type="text/json">{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="8014" rel="nofollow" href="https://www.academia.edu/Documents/in/Life_Sciences">Life Sciences</a>, <script data-card-contents-for-ri="8014" type="text/json">{"id":8014,"name":"Life Sciences","url":"https://www.academia.edu/Documents/in/Life_Sciences?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a><script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=50467502]'), work: {"id":50467502,"title":"Differential effects of mercurial compounds on excitable tissues","created_at":"2021-07-31T14:52:16.446-07:00","url":"https://www.academia.edu/50467502/Differential_effects_of_mercurial_compounds_on_excitable_tissues?f_ri=9534","dom_id":"work_50467502","summary":"Sarcoplasmic reticulum (SR), Ca *+ + Mg *+-ATPase, and Ca *+-ioncphore were obtained from white rabbit skeletal muscles. Methylmercury inhibited the Ca *+ + Mg*+-ATPase an d Ca**-transport but had no effect on the Ca*+ionophore. Mercuric chloride inhibited all three functions (i.e., ATPase, transport and ionophoric activity). The mechanism of HgC12 inhibition of the Ca*+ionophore was by competition with Ca*+ for Ca*'-ionophoric site whereas its inhibition of the enzyme ar,d Ca*+-transport was due to the blockage of essential sulfhydryl (-SH) groups. Ca*' + Mg*+-ATPase and Ca*+-transport were more sensitive to methylmercury than to HgC12. Acetylcholine receptor (AChR) was obtained for the electric organ of T.","downloadable_attachments":[{"id":68443266,"asset_id":50467502,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":46718799,"first_name":"Adil","last_name":"Shamoo","domain_name":"independent","page_name":"AdilShamoo","display_name":"Adil Shamoo","profile_url":"https://independent.academia.edu/AdilShamoo?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2513,"name":"Molecular Biology","url":"https://www.academia.edu/Documents/in/Molecular_Biology?f_ri=9534","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=9534","nofollow":true},{"id":8014,"name":"Life Sciences","url":"https://www.academia.edu/Documents/in/Life_Sciences?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":70902,"name":"Magnesium","url":"https://www.academia.edu/Documents/in/Magnesium?f_ri=9534"},{"id":90017,"name":"Mercury","url":"https://www.academia.edu/Documents/in/Mercury?f_ri=9534"},{"id":117270,"name":"Fishes","url":"https://www.academia.edu/Documents/in/Fishes?f_ri=9534"},{"id":159958,"name":"Acetylcholine","url":"https://www.academia.edu/Documents/in/Acetylcholine?f_ri=9534"},{"id":216769,"name":"Chemico-biological Interactions","url":"https://www.academia.edu/Documents/in/Chemico-biological_Interactions?f_ri=9534"},{"id":976404,"name":"Reaction Kinetics","url":"https://www.academia.edu/Documents/in/Reaction_Kinetics?f_ri=9534"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding?f_ri=9534"},{"id":1202042,"name":"Electric Conductivity","url":"https://www.academia.edu/Documents/in/Electric_Conductivity?f_ri=9534"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=9534"},{"id":2350842,"name":"Sarcoplasmic reticulum","url":"https://www.academia.edu/Documents/in/Sarcoplasmic_reticulum?f_ri=9534"},{"id":2898895,"name":"binding sites","url":"https://www.academia.edu/Documents/in/binding_sites?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_49241036" data-work_id="49241036" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/49241036/Hypovitaminosis_D_in_female_patients_with_chronic_low_back_pain">Hypovitaminosis D in female patients with chronic low back pain</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Chronic low back pain (LBP) is an extremely common problem in practice, where it is often labeled idiopathic. No sufficient studies have been conducted to analyze the contribution of hypovitaminosis D to the etiology of chronic LBP in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_49241036" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Chronic low back pain (LBP) is an extremely common problem in practice, where it is often labeled idiopathic. No sufficient studies have been conducted to analyze the contribution of hypovitaminosis D to the etiology of chronic LBP in populations wherein vitamin D deficiency is endemic. The present study was, therefore, carried out to examine hypovitaminosis D and its determinants in female patients with chronic LBP during the childbearing period. Sixty female patients complaining of LBP lasting more than 3 months were clinically studied rheumatologically and neurologically. Questionnaires and indices quantifying risk factors associated with vitamin D deficiency were utilized. Biochemical assays of serum calcium, phosphorus, alkaline phosphatase (ALP), parathormone (PTH), and 25-hydroxyvitamin D (25 OHD) were performed and compared to those of 20 matched healthy controls. The determinants of vitamin D levels in patients were examined by stepwise regression. Patients with LBP had significantly lower 25 OHD levels (p<0.05) and significantly higher PTH (p<0.05) and ALP (p<0.001) than controls, although there were no significant group differences in calcium and phosphorus. Hypovitaminosis D (25 OHD < 40 ng/ml) was found in 49/60 patients (81%) and 12/20 (60%) of controls, with an odds ratio of 2.97. Although many risk factors related to sun exposure, clothing, diet, and pregnancy were significantly correlated with vitamin D levels in patients, only limited duration of sun exposure, contributing 55% to the variance of 25 OHD, limited areas of skin exposed (13%), and increased number of pregnancies (2%), were significant determinants of vitamin D levels in patients. Despite the sunny climate, hypovitaminosis D is prevalent among Egyptian women in the childbearing period, especially those presenting with chronic LBP, where it is associated with hyperphosphatasia and hyperparathyroidism, without alterations in serum calcium. The major determinant of hypovitaminosis D in our patients is limited sun exposure.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/49241036" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="de5a00c4b6ba7e3365ea33b1d941dea4" rel="nofollow" data-download="{"attachment_id":67625107,"asset_id":49241036,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/67625107/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="193566976" href="https://independent.academia.edu/MahmoudOmran18">Mahmoud Omran</a><script data-card-contents-for-user="193566976" type="text/json">{"id":193566976,"first_name":"Mahmoud","last_name":"Omran","domain_name":"independent","page_name":"MahmoudOmran18","display_name":"Mahmoud Omran","profile_url":"https://independent.academia.edu/MahmoudOmran18?f_ri=9534","photo":"https://0.academia-photos.com/193566976/56185068/44381152/s65_mahmoud.omran.png"}</script></span></span></li><li class="js-paper-rank-work_49241036 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="49241036"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 49241036, container: ".js-paper-rank-work_49241036", }); 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No sufficient studies have been conducted to analyze the contribution of hypovitaminosis D to the etiology of chronic LBP in populations wherein vitamin D deficiency is endemic. The present study was, therefore, carried out to examine hypovitaminosis D and its determinants in female patients with chronic LBP during the childbearing period. Sixty female patients complaining of LBP lasting more than 3 months were clinically studied rheumatologically and neurologically. Questionnaires and indices quantifying risk factors associated with vitamin D deficiency were utilized. Biochemical assays of serum calcium, phosphorus, alkaline phosphatase (ALP), parathormone (PTH), and 25-hydroxyvitamin D (25 OHD) were performed and compared to those of 20 matched healthy controls. The determinants of vitamin D levels in patients were examined by stepwise regression. Patients with LBP had significantly lower 25 OHD levels (p\u003c0.05) and significantly higher PTH (p\u003c0.05) and ALP (p\u003c0.001) than controls, although there were no significant group differences in calcium and phosphorus. Hypovitaminosis D (25 OHD \u003c 40 ng/ml) was found in 49/60 patients (81%) and 12/20 (60%) of controls, with an odds ratio of 2.97. Although many risk factors related to sun exposure, clothing, diet, and pregnancy were significantly correlated with vitamin D levels in patients, only limited duration of sun exposure, contributing 55% to the variance of 25 OHD, limited areas of skin exposed (13%), and increased number of pregnancies (2%), were significant determinants of vitamin D levels in patients. Despite the sunny climate, hypovitaminosis D is prevalent among Egyptian women in the childbearing period, especially those presenting with chronic LBP, where it is associated with hyperphosphatasia and hyperparathyroidism, without alterations in serum calcium. The major determinant of hypovitaminosis D in our patients is limited sun exposure.","downloadable_attachments":[{"id":67625107,"asset_id":49241036,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":193566976,"first_name":"Mahmoud","last_name":"Omran","domain_name":"independent","page_name":"MahmoudOmran18","display_name":"Mahmoud Omran","profile_url":"https://independent.academia.edu/MahmoudOmran18?f_ri=9534","photo":"https://0.academia-photos.com/193566976/56185068/44381152/s65_mahmoud.omran.png"}],"research_interests":[{"id":9334,"name":"Inflammation","url":"https://www.academia.edu/Documents/in/Inflammation?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":40760,"name":"Low back pain","url":"https://www.academia.edu/Documents/in/Low_back_pain?f_ri=9534","nofollow":true},{"id":53108,"name":"Phosphorus","url":"https://www.academia.edu/Documents/in/Phosphorus?f_ri=9534","nofollow":true},{"id":62550,"name":"Pregnancy","url":"https://www.academia.edu/Documents/in/Pregnancy?f_ri=9534"},{"id":97269,"name":"Chronic Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease?f_ri=9534"},{"id":99708,"name":"Clinical","url":"https://www.academia.edu/Documents/in/Clinical?f_ri=9534"},{"id":123230,"name":"Regression Analysis","url":"https://www.academia.edu/Documents/in/Regression_Analysis?f_ri=9534"},{"id":130232,"name":"OSTEOMALACIA","url":"https://www.academia.edu/Documents/in/OSTEOMALACIA?f_ri=9534"},{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=9534"},{"id":204435,"name":"Alkaline phosphatase","url":"https://www.academia.edu/Documents/in/Alkaline_phosphatase?f_ri=9534"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":327850,"name":"Questionnaires","url":"https://www.academia.edu/Documents/in/Questionnaires?f_ri=9534"},{"id":437772,"name":"Odds ratio","url":"https://www.academia.edu/Documents/in/Odds_ratio?f_ri=9534"},{"id":546356,"name":"Clinical Study","url":"https://www.academia.edu/Documents/in/Clinical_Study?f_ri=9534"},{"id":620049,"name":"Risk Factors","url":"https://www.academia.edu/Documents/in/Risk_Factors-1?f_ri=9534"},{"id":863963,"name":"Chronic Low Back Pain","url":"https://www.academia.edu/Documents/in/Chronic_Low_Back_Pain?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_47365948" data-work_id="47365948" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/47365948/The_gadolinium_ion_a_potent_blocker_of_calcium_channels_and_catecholamine_release_from_cultured_chromaffin_cells">The gadolinium ion: a potent blocker of calcium channels and catecholamine release from cultured chromaffin cells</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest">ions, Ca" entry and catechol~imine release 1621 A~~,loi~ieil!leltrenr.s---We thank Mrs J. Ritchie and Mrs A. This work was sttpporte~~ by grants from The Medical</div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/47365948" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cb67d8a561d3c7fa85f9b32f9c8f9337" rel="nofollow" data-download="{"attachment_id":66495449,"asset_id":47365948,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/66495449/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="132294438" href="https://independent.academia.edu/JoseMariaTrifaro">Jose-Maria Trifaro</a><script data-card-contents-for-user="132294438" type="text/json">{"id":132294438,"first_name":"Jose-Maria","last_name":"Trifaro","domain_name":"independent","page_name":"JoseMariaTrifaro","display_name":"Jose-Maria Trifaro","profile_url":"https://independent.academia.edu/JoseMariaTrifaro?f_ri=9534","photo":"https://0.academia-photos.com/132294438/71390942/59834415/s65_jose-maria.trifaro.png"}</script></span></span></li><li class="js-paper-rank-work_47365948 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="47365948"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 47365948, container: ".js-paper-rank-work_47365948", }); 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$(".js-view-count[data-work-id=47365948]").text(description); $(".js-view-count-work_47365948").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_47365948").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="47365948"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="161" rel="nofollow" href="https://www.academia.edu/Documents/in/Neuroscience">Neuroscience</a>, <script data-card-contents-for-ri="161" type="text/json">{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="221" rel="nofollow" href="https://www.academia.edu/Documents/in/Psychology">Psychology</a>, <script data-card-contents-for-ri="221" type="text/json">{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="31084" rel="nofollow" href="https://www.academia.edu/Documents/in/Ion_Channels">Ion Channels</a><script data-card-contents-for-ri="31084" type="text/json">{"id":31084,"name":"Ion Channels","url":"https://www.academia.edu/Documents/in/Ion_Channels?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=47365948]'), work: {"id":47365948,"title":"The gadolinium ion: a potent blocker of calcium channels and catecholamine release from cultured chromaffin cells","created_at":"2021-04-21T16:33:53.434-07:00","url":"https://www.academia.edu/47365948/The_gadolinium_ion_a_potent_blocker_of_calcium_channels_and_catecholamine_release_from_cultured_chromaffin_cells?f_ri=9534","dom_id":"work_47365948","summary":"ions, Ca\" entry and catechol~imine release 1621 A~~,loi~ieil!leltrenr.s---We thank Mrs J. Ritchie and Mrs A. This work was sttpporte~~ by grants from The Medical","downloadable_attachments":[{"id":66495449,"asset_id":47365948,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":132294438,"first_name":"Jose-Maria","last_name":"Trifaro","domain_name":"independent","page_name":"JoseMariaTrifaro","display_name":"Jose-Maria Trifaro","profile_url":"https://independent.academia.edu/JoseMariaTrifaro?f_ri=9534","photo":"https://0.academia-photos.com/132294438/71390942/59834415/s65_jose-maria.trifaro.png"}],"research_interests":[{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=9534","nofollow":true},{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":31084,"name":"Ion Channels","url":"https://www.academia.edu/Documents/in/Ion_Channels?f_ri=9534","nofollow":true},{"id":159958,"name":"Acetylcholine","url":"https://www.academia.edu/Documents/in/Acetylcholine?f_ri=9534"},{"id":160656,"name":"Potassium","url":"https://www.academia.edu/Documents/in/Potassium?f_ri=9534"},{"id":235189,"name":"Norepinephrine","url":"https://www.academia.edu/Documents/in/Norepinephrine?f_ri=9534"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle?f_ri=9534"},{"id":386290,"name":"Gadolinium","url":"https://www.academia.edu/Documents/in/Gadolinium?f_ri=9534"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=9534"},{"id":2329699,"name":"Adrenal Medulla","url":"https://www.academia.edu/Documents/in/Adrenal_Medulla?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_5627577" data-work_id="5627577" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/5627577/Endothelin1_stimulates_insulin_secretion_by_direct_action_on_the_islets_of_Langerhans_in_mice">Endothelin1 stimulates insulin secretion by direct action on the islets of Langerhans in mice</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Endothelin-1 (ET-1), a potent endothelium-derived vasoconstrictor peptide, is secreted in response to insulin. Elevated circulating ET-1 levels have been found in patients with diabetes mellitus and vascular dysfunction. The question... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5627577" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Endothelin-1 (ET-1), a potent endothelium-derived vasoconstrictor peptide, is secreted in response to insulin. Elevated circulating ET-1 levels have been found in patients with diabetes mellitus and vascular dysfunction. The question arises whether ET-1 acts as a direct modulator of insulin secretion. To test this, we studied the effects of ET-1 on isolated mouse islets of Langerhans. ET-1 (1 nmol/l-1 Μmol/l) dose-dependently stimulated insulin secretion from islets incubated in the presence of 16.7 mmol/l glucose (p<0.05). The effect of ET-1 is glucose-dependent since no potentiation was found at 3.3 mmol/l glucose. Furthermore, ET-1 induced a large, transient increase in glucose-stimulated insulin secretion during islet perifusion in the presence (p<0.001), but not in the absence, of extracellular Ca2+. The rate of 45Ca2+-efflux from 45Ca2+-prelabelled islets was transiently stimulated by ET-1 during perifusion at 16.7 mmol/l glucose in the presence of extracellular Ca2+ (p<0.001). A short-lived increase in 45Ca2+-efflux was also observed in the absence of extracellular Ca2+ (p<0.05). It is suggested that the effects of ET-1 on insulin secretion are critically dependent on influx via Ca2+-channels. In addition, ET-1 transiently enhanced 86Rb+-efflux from 86Rb+-prelabelled islets both in the presence (p<0.001) and in the absence (p<0.001) of extracellular Ca2+ suggesting that ET-1 does not elicit insulin secretion by inhibition of the potassium permeability. Our study provides evidence that ET-1 stimulates insulin secretion via a direct effect on the islets of Langerhans.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5627577" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1f620b9cccff5b806d65767e1fea927d" rel="nofollow" data-download="{"attachment_id":49208635,"asset_id":5627577,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49208635/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="8074246" href="https://rm.academia.edu/kjeldhermansen">kjeld hermansen</a><script data-card-contents-for-user="8074246" type="text/json">{"id":8074246,"first_name":"kjeld","last_name":"hermansen","domain_name":"rm","page_name":"kjeldhermansen","display_name":"kjeld hermansen","profile_url":"https://rm.academia.edu/kjeldhermansen?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_5627577 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5627577"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5627577, container: ".js-paper-rank-work_5627577", }); 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Elevated circulating ET-1 levels have been found in patients with diabetes mellitus and vascular dysfunction. The question arises whether ET-1 acts as a direct modulator of insulin secretion. To test this, we studied the effects of ET-1 on isolated mouse islets of Langerhans. ET-1 (1 nmol/l-1 Μmol/l) dose-dependently stimulated insulin secretion from islets incubated in the presence of 16.7 mmol/l glucose (p\u003c0.05). The effect of ET-1 is glucose-dependent since no potentiation was found at 3.3 mmol/l glucose. Furthermore, ET-1 induced a large, transient increase in glucose-stimulated insulin secretion during islet perifusion in the presence (p\u003c0.001), but not in the absence, of extracellular Ca2+. The rate of 45Ca2+-efflux from 45Ca2+-prelabelled islets was transiently stimulated by ET-1 during perifusion at 16.7 mmol/l glucose in the presence of extracellular Ca2+ (p\u003c0.001). A short-lived increase in 45Ca2+-efflux was also observed in the absence of extracellular Ca2+ (p\u003c0.05). It is suggested that the effects of ET-1 on insulin secretion are critically dependent on influx via Ca2+-channels. In addition, ET-1 transiently enhanced 86Rb+-efflux from 86Rb+-prelabelled islets both in the presence (p\u003c0.001) and in the absence (p\u003c0.001) of extracellular Ca2+ suggesting that ET-1 does not elicit insulin secretion by inhibition of the potassium permeability. Our study provides evidence that ET-1 stimulates insulin secretion via a direct effect on the islets of Langerhans.","downloadable_attachments":[{"id":49208635,"asset_id":5627577,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":8074246,"first_name":"kjeld","last_name":"hermansen","domain_name":"rm","page_name":"kjeldhermansen","display_name":"kjeld hermansen","profile_url":"https://rm.academia.edu/kjeldhermansen?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":71289,"name":"Glucose","url":"https://www.academia.edu/Documents/in/Glucose?f_ri=9534","nofollow":true},{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin?f_ri=9534","nofollow":true},{"id":71426,"name":"Insulin Secretion","url":"https://www.academia.edu/Documents/in/Insulin_Secretion?f_ri=9534"},{"id":71511,"name":"Diabetes mellitus","url":"https://www.academia.edu/Documents/in/Diabetes_mellitus?f_ri=9534"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534"},{"id":160656,"name":"Potassium","url":"https://www.academia.edu/Documents/in/Potassium?f_ri=9534"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":410370,"name":"Public health systems and services research","url":"https://www.academia.edu/Documents/in/Public_health_systems_and_services_research-1?f_ri=9534"},{"id":413194,"name":"Analysis of Variance","url":"https://www.academia.edu/Documents/in/Analysis_of_Variance?f_ri=9534"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=9534"},{"id":539690,"name":"Endothelin-1","url":"https://www.academia.edu/Documents/in/Endothelin-1?f_ri=9534"},{"id":1705044,"name":"islets of Langerhans","url":"https://www.academia.edu/Documents/in/islets_of_Langerhans?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_8654439" data-work_id="8654439" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/8654439/Characterization_of_an_extracellular_polysaccharide_produced_by_a_Pseudomonas_strain_grown_on_glycerol">Characterization of an extracellular polysaccharide produced by a Pseudomonas strain grown on glycerol</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A new extracellular charged polysaccharide composed mainly by galactose, with lower amounts of mannose, glucose and rhamnose, was produced by the cultivation of Pseudomonas oleovorans NRRL B-14682 using glycerol as the sole carbon source.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_8654439" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A new extracellular charged polysaccharide composed mainly by galactose, with lower amounts of mannose, glucose and rhamnose, was produced by the cultivation of Pseudomonas oleovorans NRRL B-14682 using glycerol as the sole carbon source. Thermal and solid-state NMR analysis showed that this polymer is essentially amorphous, with a glass transition temperature of 155.7°C. The exopolysaccharide aqueous solutions have viscoelastic properties similar to that of Guar gum, but with affinity to salts as a result of its polyelectrolyte character. In addition, the exopolysaccharide has demonstrated good flocculating and emulsifying properties and film-forming capacity. These properties make this polymer a good alternative to more expensive natural polysaccharides, such as Guar gum, in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/8654439" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1296a12ea20df725a1c98518d104e0d7" rel="nofollow" data-download="{"attachment_id":48029931,"asset_id":8654439,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48029931/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="18059818" href="https://independent.academia.edu/CristinaOliveira23">Cristina Oliveira</a><script data-card-contents-for-user="18059818" type="text/json">{"id":18059818,"first_name":"Cristina","last_name":"Oliveira","domain_name":"independent","page_name":"CristinaOliveira23","display_name":"Cristina Oliveira","profile_url":"https://independent.academia.edu/CristinaOliveira23?f_ri=9534","photo":"https://0.academia-photos.com/18059818/5032044/5774785/s65_cristina.oliveira.jpg_oh_e0f7787f6dc1664d972214c80e706470_oe_54c2d288___gda___1422024197_542367c4bffaf9d168081ccaba33ef35"}</script></span></span></li><li class="js-paper-rank-work_8654439 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="8654439"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 8654439, container: ".js-paper-rank-work_8654439", }); 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Thermal and solid-state NMR analysis showed that this polymer is essentially amorphous, with a glass transition temperature of 155.7°C. The exopolysaccharide aqueous solutions have viscoelastic properties similar to that of Guar gum, but with affinity to salts as a result of its polyelectrolyte character. In addition, the exopolysaccharide has demonstrated good flocculating and emulsifying properties and film-forming capacity. These properties make this polymer a good alternative to more expensive natural polysaccharides, such as Guar gum, in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries.","downloadable_attachments":[{"id":48029931,"asset_id":8654439,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":18059818,"first_name":"Cristina","last_name":"Oliveira","domain_name":"independent","page_name":"CristinaOliveira23","display_name":"Cristina Oliveira","profile_url":"https://independent.academia.edu/CristinaOliveira23?f_ri=9534","photo":"https://0.academia-photos.com/18059818/5032044/5774785/s65_cristina.oliveira.jpg_oh_e0f7787f6dc1664d972214c80e706470_oe_54c2d288___gda___1422024197_542367c4bffaf9d168081ccaba33ef35"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=9534","nofollow":true},{"id":531,"name":"Organic 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Source","url":"https://www.academia.edu/Documents/in/Carbon_Source?f_ri=9534"},{"id":1323439,"name":"Carbohydrate Polymers","url":"https://www.academia.edu/Documents/in/Carbohydrate_Polymers?f_ri=9534"},{"id":1499550,"name":"Thermal gravimetric analysis","url":"https://www.academia.edu/Documents/in/Thermal_gravimetric_analysis?f_ri=9534"},{"id":1991776,"name":"Viscoelastic Properties","url":"https://www.academia.edu/Documents/in/Viscoelastic_Properties?f_ri=9534"},{"id":2230296,"name":"Guar Gum","url":"https://www.academia.edu/Documents/in/Guar_Gum?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24433039" data-work_id="24433039" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24433039/Chromatin_decondensation_pronucleus_formation_metaphase_entry_and_chromosome_complements_of_human_spermatozoa_after_intracytoplasmic_sperm_injection_into_hamster_oocytes">Chromatin decondensation, pronucleus formation, metaphase entry and chromosome complements of human spermatozoa after intracytoplasmic sperm injection into hamster oocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Obtaining karyotypes from human spermatozoa after microinjection into Syrian golden hamster oocytes is difficult and the hitherto reported results are unsatisfactory. This may be related to the injection and culture technique or to the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24433039" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Obtaining karyotypes from human spermatozoa after microinjection into Syrian golden hamster oocytes is difficult and the hitherto reported results are unsatisfactory. This may be related to the injection and culture technique or to the high susceptibility of the hamster oocytes to undergo parthenogenetic activation or both. Therefore, we investigated the hamster oocyte-human sperm microinjection model using the following two approaches: (i) application of contemporary techniques for injection (touching the sperm tail) and culture (hamster embryo culture medium, HECM-3, 10% CO 2 ) and (ii) omission of Ca 2⍣ from the injection medium. Thus, in the first series of experiments, 252 hamster oocytes were injected with human spermatozoa. Among the 219 (87%) oocytes that survived the injection procedure, the mean percentages of male pronucleus formation [two pronuclei (2PN), two polar bodies (PB)], mitotic metaphase entry and sperm chromosome spreads were 41.4, 27.8 and 18.2% respectively. Analysis of the oocytes which failed to develop the male pronucleus following injection revealed that most of them had developed only the hamster female PN while the sperm nuclei were either intact or swollen (partially decondensed), indicating that failure of oocyte activation was not the likely reason for the failure of male PN formation in these oocytes. In the next series of experiments, sibling oocytes were alternately injected with spermatozoa suspended either in the regular (1.9 mM Ca 2⍣ ) or Ca 2⍣ -free injection medium (experiment set 2, n ⍧ 278). A significant improvement was noted in the mean percentages of oocytes with 2PN, 2PB, metaphase entry and sperm chromosome spreads in the Ca 2⍣ -free group versus the regular group (2PN, 2PB: 51 versus 36.6%, metaphase entry: 36.3 versus 26.9% and sperm chromosome spreads: 28 versus 20.4%; all P < 0.04). Thus, parthenogenetic activation appears to be one of the contributing factors for the failure of male PN formation after heterospecific hamster ICSI. From these experiments it can be concluded that application of the advanced injection and culture techniques and omission of Ca 2⍣ from the injection medium are promising for the routine application of the hamster oocyte microinjection for karyotyping of human spermatozoa with poor fertilizing capacity.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24433039" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2841a16d266f395357e0a03bf44568fd" rel="nofollow" data-download="{"attachment_id":44762623,"asset_id":24433039,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44762623/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="47098804" href="https://independent.academia.edu/MarcDhont">Marc Dhont</a><script data-card-contents-for-user="47098804" type="text/json">{"id":47098804,"first_name":"Marc","last_name":"Dhont","domain_name":"independent","page_name":"MarcDhont","display_name":"Marc Dhont","profile_url":"https://independent.academia.edu/MarcDhont?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24433039 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24433039"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24433039, container: ".js-paper-rank-work_24433039", }); 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This may be related to the injection and culture technique or to the high susceptibility of the hamster oocytes to undergo parthenogenetic activation or both. Therefore, we investigated the hamster oocyte-human sperm microinjection model using the following two approaches: (i) application of contemporary techniques for injection (touching the sperm tail) and culture (hamster embryo culture medium, HECM-3, 10% CO 2 ) and (ii) omission of Ca 2⍣ from the injection medium. Thus, in the first series of experiments, 252 hamster oocytes were injected with human spermatozoa. Among the 219 (87%) oocytes that survived the injection procedure, the mean percentages of male pronucleus formation [two pronuclei (2PN), two polar bodies (PB)], mitotic metaphase entry and sperm chromosome spreads were 41.4, 27.8 and 18.2% respectively. Analysis of the oocytes which failed to develop the male pronucleus following injection revealed that most of them had developed only the hamster female PN while the sperm nuclei were either intact or swollen (partially decondensed), indicating that failure of oocyte activation was not the likely reason for the failure of male PN formation in these oocytes. In the next series of experiments, sibling oocytes were alternately injected with spermatozoa suspended either in the regular (1.9 mM Ca 2⍣ ) or Ca 2⍣ -free injection medium (experiment set 2, n ⍧ 278). A significant improvement was noted in the mean percentages of oocytes with 2PN, 2PB, metaphase entry and sperm chromosome spreads in the Ca 2⍣ -free group versus the regular group (2PN, 2PB: 51 versus 36.6%, metaphase entry: 36.3 versus 26.9% and sperm chromosome spreads: 28 versus 20.4%; all P \u003c 0.04). Thus, parthenogenetic activation appears to be one of the contributing factors for the failure of male PN formation after heterospecific hamster ICSI. From these experiments it can be concluded that application of the advanced injection and culture techniques and omission of Ca 2⍣ from the injection medium are promising for the routine application of the hamster oocyte microinjection for karyotyping of human spermatozoa with poor fertilizing capacity.","downloadable_attachments":[{"id":44762623,"asset_id":24433039,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":47098804,"first_name":"Marc","last_name":"Dhont","domain_name":"independent","page_name":"MarcDhont","display_name":"Marc Dhont","profile_url":"https://independent.academia.edu/MarcDhont?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":65133,"name":"Parthenogenesis","url":"https://www.academia.edu/Documents/in/Parthenogenesis?f_ri=9534","nofollow":true},{"id":98929,"name":"Intracytoplasmic Sperm Injection","url":"https://www.academia.edu/Documents/in/Intracytoplasmic_Sperm_Injection?f_ri=9534","nofollow":true},{"id":153639,"name":"Karyotyping","url":"https://www.academia.edu/Documents/in/Karyotyping?f_ri=9534","nofollow":true},{"id":178928,"name":"Male Infertility","url":"https://www.academia.edu/Documents/in/Male_Infertility?f_ri=9534"},{"id":238813,"name":"Chromatin","url":"https://www.academia.edu/Documents/in/Chromatin?f_ri=9534"},{"id":291842,"name":"Human Spermatozoa","url":"https://www.academia.edu/Documents/in/Human_Spermatozoa?f_ri=9534"},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus?f_ri=9534"},{"id":351108,"name":"Human reproduction","url":"https://www.academia.edu/Documents/in/Human_reproduction?f_ri=9534"},{"id":613442,"name":"Fertilization in Vitro","url":"https://www.academia.edu/Documents/in/Fertilization_in_Vitro?f_ri=9534"},{"id":862371,"name":"Spermatozoa","url":"https://www.academia.edu/Documents/in/Spermatozoa?f_ri=9534"},{"id":956315,"name":"Oocytes","url":"https://www.academia.edu/Documents/in/Oocytes?f_ri=9534"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=9534"},{"id":1166930,"name":"Cytoplasm","url":"https://www.academia.edu/Documents/in/Cytoplasm?f_ri=9534"},{"id":2111545,"name":"Spontaneous Activity","url":"https://www.academia.edu/Documents/in/Spontaneous_Activity?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14832142" data-work_id="14832142" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14832142/Alendronate_affects_calcium_dynamics_in_cardiomyocytes_in_vitro">Alendronate affects calcium dynamics in cardiomyocytes in vitro</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Therapy with bisphosphonates, including alendronate (ALN), is considered a safe and effective treatment for osteoporosis. However, recent studies have reported an unexpected increase in serious atrial fibrillation (AF) in patients treated... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14832142" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Therapy with bisphosphonates, including alendronate (ALN), is considered a safe and effective treatment for osteoporosis. However, recent studies have reported an unexpected increase in serious atrial fibrillation (AF) in patients treated with bisphosphonates. The mechanism that explains this side effect remains unknown. Since AF is associated with an altered sarcoendoplasmic reticulum calcium load, we studied how ALN affects cardiomyocyte calcium homeostasis and protein isoprenylation in vitro. Acute and long-term (48 h) treatment of atrial and ventricular cardiomyocytes with ALN (10 − 8 -10 − 6 M) was performed. Changes in calcium dynamics were determined by both fluorescence measurement of cytosolic free Ca 2+ concentration and western blot analysis of calcium-regulating proteins. Finally, effect of ALN on protein farnesylation was also identified. In both atrial and ventricular cardiomyocytes, ALN treatment delayed and diminished calcium responses to caffeine. Only in atrial cells, long-term exposure to ALN-induced transitory calcium oscillations and led to the development of oscillatory component in calcium responses to caffeine. Changes in calcium dynamics were accompanied by changes in expression of proteins controlling sarcoendoplasmic reticulum calcium. In contrast, ALN minimally affected protein isoprenylation in these cells. In summary, treatment of atrial cardiomyocytes with ALN-induced abnormalities in calcium dynamics consistent with induction of a self-stimulatory, pacemaker-like behavior, which may contribute to the development of cardiac side effects associated with these drugs.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14832142" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5f92c6e9580b77b3c90d44b89bba075c" rel="nofollow" data-download="{"attachment_id":43867261,"asset_id":14832142,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43867261/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33791310" href="https://independent.academia.edu/LorraineChalifour">Lorraine Chalifour</a><script data-card-contents-for-user="33791310" type="text/json">{"id":33791310,"first_name":"Lorraine","last_name":"Chalifour","domain_name":"independent","page_name":"LorraineChalifour","display_name":"Lorraine Chalifour","profile_url":"https://independent.academia.edu/LorraineChalifour?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_14832142 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="14832142"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 14832142, container: ".js-paper-rank-work_14832142", }); 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However, recent studies have reported an unexpected increase in serious atrial fibrillation (AF) in patients treated with bisphosphonates. The mechanism that explains this side effect remains unknown. Since AF is associated with an altered sarcoendoplasmic reticulum calcium load, we studied how ALN affects cardiomyocyte calcium homeostasis and protein isoprenylation in vitro. Acute and long-term (48 h) treatment of atrial and ventricular cardiomyocytes with ALN (10 − 8 -10 − 6 M) was performed. Changes in calcium dynamics were determined by both fluorescence measurement of cytosolic free Ca 2+ concentration and western blot analysis of calcium-regulating proteins. Finally, effect of ALN on protein farnesylation was also identified. In both atrial and ventricular cardiomyocytes, ALN treatment delayed and diminished calcium responses to caffeine. Only in atrial cells, long-term exposure to ALN-induced transitory calcium oscillations and led to the development of oscillatory component in calcium responses to caffeine. Changes in calcium dynamics were accompanied by changes in expression of proteins controlling sarcoendoplasmic reticulum calcium. In contrast, ALN minimally affected protein isoprenylation in these cells. In summary, treatment of atrial cardiomyocytes with ALN-induced abnormalities in calcium dynamics consistent with induction of a self-stimulatory, pacemaker-like behavior, which may contribute to the development of cardiac side effects associated with these drugs.","downloadable_attachments":[{"id":43867261,"asset_id":14832142,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33791310,"first_name":"Lorraine","last_name":"Chalifour","domain_name":"independent","page_name":"LorraineChalifour","display_name":"Lorraine Chalifour","profile_url":"https://independent.academia.edu/LorraineChalifour?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":67129,"name":"Caffeine","url":"https://www.academia.edu/Documents/in/Caffeine?f_ri=9534","nofollow":true},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534","nofollow":true},{"id":130890,"name":"Atrial Fibrillation","url":"https://www.academia.edu/Documents/in/Atrial_Fibrillation?f_ri=9534","nofollow":true},{"id":228722,"name":"Vascular","url":"https://www.academia.edu/Documents/in/Vascular?f_ri=9534"},{"id":272426,"name":"Alendronate","url":"https://www.academia.edu/Documents/in/Alendronate?f_ri=9534"},{"id":293675,"name":"Calcium Homeostasis","url":"https://www.academia.edu/Documents/in/Calcium_Homeostasis?f_ri=9534"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot?f_ri=9534"},{"id":698785,"name":"Side Effect","url":"https://www.academia.edu/Documents/in/Side_Effect?f_ri=9534"},{"id":746681,"name":"Arrhythmia","url":"https://www.academia.edu/Documents/in/Arrhythmia?f_ri=9534"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation?f_ri=9534"},{"id":788661,"name":"Vascular Pharmacology","url":"https://www.academia.edu/Documents/in/Vascular_Pharmacology?f_ri=9534"},{"id":1348796,"name":"Dynamic Consistency","url":"https://www.academia.edu/Documents/in/Dynamic_Consistency?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_23363840" data-work_id="23363840" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/23363840/Arsenic_stress_elicits_cytosolic_Ca2_bursts_and_Crz1_activation_in_Saccharomyces_cerevisiae">Arsenic stress elicits cytosolic Ca2+ bursts and Crz1 activation in Saccharomyces cerevisiae</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Although arsenic is notoriously poisonous to life, its utilization in therapeutics brings many benefits to human health, so it is therefore essential to discover the molecular mechanisms underlying arsenic stress responses in eukaryotic... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23363840" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Although arsenic is notoriously poisonous to life, its utilization in therapeutics brings many benefits to human health, so it is therefore essential to discover the molecular mechanisms underlying arsenic stress responses in eukaryotic cells. Aiming to determine the contribution of Ca 2+ signalling pathways to arsenic stress responses, we took advantage of the use of Saccharomyces cerevisiae as a model organism. Here we show that Ca 2+ enhances the tolerance of the wild-type and arsenic-sensitive yap1 strains to arsenic stress in a Crz1-dependent manner, thus providing the first evidence that Ca 2+ signalling cascades are involved in arsenic stress responses. Moreover, our results indicate that arsenic shock elicits a cytosolic Ca 2+ burst in these strains, without the addition of exogenous Ca 2+ sources, strongly supporting the notion that Ca 2+ homeostasis is disrupted by arsenic stress. In response to an arsenite-induced increase of Ca 2+ in the cytosol, Crz1 is dephosphorylated and translocated to the nucleus, and stimulates CDREdriven expression of the lacZ reporter gene in a Cnb1-dependent manner. The activation of Crz1 by arsenite culminates in the induction of the endogenous genes PMR1, PMC1 and GSC2. Taken together, these data establish that activation of Ca 2+ signalling pathways and the downstream activation of the Crz1 transcription factor contribute to arsenic tolerance in the eukaryotic model organism S. cerevisiae.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23363840" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bb455a3024500afe1f4c4533ca8d41fc" rel="nofollow" data-download="{"attachment_id":43817119,"asset_id":23363840,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43817119/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="45217354" href="https://unl-pt.academia.edu/ReginaMenezes">Regina Menezes</a><script data-card-contents-for-user="45217354" type="text/json">{"id":45217354,"first_name":"Regina","last_name":"Menezes","domain_name":"unl-pt","page_name":"ReginaMenezes","display_name":"Regina Menezes","profile_url":"https://unl-pt.academia.edu/ReginaMenezes?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_23363840 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23363840"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23363840, container: ".js-paper-rank-work_23363840", }); 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$(".js-view-count[data-work-id=23363840]").text(description); $(".js-view-count-work_23363840").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_23363840").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="23363840"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6227" rel="nofollow" href="https://www.academia.edu/Documents/in/Arsenic">Arsenic</a>, <script data-card-contents-for-ri="6227" type="text/json">{"id":6227,"name":"Arsenic","url":"https://www.academia.edu/Documents/in/Arsenic?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="23323" rel="nofollow" href="https://www.academia.edu/Documents/in/Transcription_Factors">Transcription Factors</a><script data-card-contents-for-ri="23323" type="text/json">{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=23363840]'), work: {"id":23363840,"title":"Arsenic stress elicits cytosolic Ca2+ bursts and Crz1 activation in Saccharomyces cerevisiae","created_at":"2016-03-17T05:16:54.928-07:00","url":"https://www.academia.edu/23363840/Arsenic_stress_elicits_cytosolic_Ca2_bursts_and_Crz1_activation_in_Saccharomyces_cerevisiae?f_ri=9534","dom_id":"work_23363840","summary":"Although arsenic is notoriously poisonous to life, its utilization in therapeutics brings many benefits to human health, so it is therefore essential to discover the molecular mechanisms underlying arsenic stress responses in eukaryotic cells. Aiming to determine the contribution of Ca 2+ signalling pathways to arsenic stress responses, we took advantage of the use of Saccharomyces cerevisiae as a model organism. Here we show that Ca 2+ enhances the tolerance of the wild-type and arsenic-sensitive yap1 strains to arsenic stress in a Crz1-dependent manner, thus providing the first evidence that Ca 2+ signalling cascades are involved in arsenic stress responses. Moreover, our results indicate that arsenic shock elicits a cytosolic Ca 2+ burst in these strains, without the addition of exogenous Ca 2+ sources, strongly supporting the notion that Ca 2+ homeostasis is disrupted by arsenic stress. In response to an arsenite-induced increase of Ca 2+ in the cytosol, Crz1 is dephosphorylated and translocated to the nucleus, and stimulates CDREdriven expression of the lacZ reporter gene in a Cnb1-dependent manner. The activation of Crz1 by arsenite culminates in the induction of the endogenous genes PMR1, PMC1 and GSC2. Taken together, these data establish that activation of Ca 2+ signalling pathways and the downstream activation of the Crz1 transcription factor contribute to arsenic tolerance in the eukaryotic model organism S. cerevisiae.","downloadable_attachments":[{"id":43817119,"asset_id":23363840,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":45217354,"first_name":"Regina","last_name":"Menezes","domain_name":"unl-pt","page_name":"ReginaMenezes","display_name":"Regina Menezes","profile_url":"https://unl-pt.academia.edu/ReginaMenezes?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=9534","nofollow":true},{"id":6227,"name":"Arsenic","url":"https://www.academia.edu/Documents/in/Arsenic?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors?f_ri=9534","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=9534"},{"id":49123,"name":"Saccharomyces cerevisiae","url":"https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae?f_ri=9534"},{"id":722449,"name":"Physiological Stress Markers","url":"https://www.academia.edu/Documents/in/Physiological_Stress_Markers?f_ri=9534"},{"id":1186610,"name":"DNA binding proteins","url":"https://www.academia.edu/Documents/in/DNA_binding_proteins?f_ri=9534"},{"id":1810445,"name":"Gene expression profiling","url":"https://www.academia.edu/Documents/in/Gene_expression_profiling?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_23288316 coauthored" data-work_id="23288316" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/23288316/Sunitinib_induced_hyperparathyroidism">Sunitinib-induced hyperparathyroidism</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">BACKGROUND: Sunitinib malate is an orally bioavailable tyrosine kinase inhibitor that is active against many tyrosine kinase receptors involving crucial pathways in both healthy tissues and malignant tissues. Because its use in clinical... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23288316" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">BACKGROUND: Sunitinib malate is an orally bioavailable tyrosine kinase inhibitor that is active against many tyrosine kinase receptors involving crucial pathways in both healthy tissues and malignant tissues. Because its use in clinical practice is quite recent, many of its possible side effects remain unknown. In this report, the authors describe the incidence of new-onset hyperparathyroidism in a cohort of patients with metastatic renal cell carcinoma who received treatment with sunitinib. METHODS: Twenty-six patients who received first-line sunitinib for metastatic renal cell carcinoma were enrolled in this study for a mineral and parathyroid function assessment. Plasma levels of intact parathyroid hormone; serum levels of calcium, phosphorus, 25-hydroxyvitamin D 3 , and 1,25-dihydrovitamin D 3 ; and urinary 24-hour calcium and phosphorus excretion all were measured in each patient. Biochemical evaluations were performed before the beginning of treatment and at the end of each sunitinib treatment period. RESULTS: Eighteen of 26 patients (69.2%) developed hyperparathyroidism with normal serum calcium levels, and 6 of them developed hypophosphatemia. Patients presented with a mean elevation of parathyroid hormone after 2.2 cycles of sunitinib. The levels of 25-OH vitamin D 3 were stable over the course of treatment, whereas 1,25-OH vitamin D 3 levels were increased in 5 hyperparathyroid patients. Those who presenting with elevated parathyroid hormone levels had low or undetectable urinary calcium levels. Parathyroid hormone elevation usually persisted but did not progress during long-term therapy with sunitinib. Permanent treatment interruption resulted in a resolution of hyperparathyroidism. CONCLUSIONS: Hyperparathyroidism developed in an high percentage of patients on sunitinib. Therefore, the authors concluded that sunitinib may affect parathyroid function and bone mineral homeostasis, possibly resulting in abnormal bone remodeling. Cancer 2012;118:3165-72</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23288316" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4f4f44fd79593079c161fc4204c072f2" rel="nofollow" data-download="{"attachment_id":43756228,"asset_id":23288316,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43756228/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="45174342" href="https://independent.academia.edu/AlbertoLapini">Alberto Lapini</a><script data-card-contents-for-user="45174342" type="text/json">{"id":45174342,"first_name":"Alberto","last_name":"Lapini","domain_name":"independent","page_name":"AlbertoLapini","display_name":"Alberto Lapini","profile_url":"https://independent.academia.edu/AlbertoLapini?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-23288316">+1</span><div class="hidden js-additional-users-23288316"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/BaldazziValentina">Valentina Baldazzi</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-23288316'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-23288316').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_23288316 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23288316"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23288316, container: ".js-paper-rank-work_23288316", }); });</script></li><li class="js-percentile-work_23288316 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 23288316; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_23288316"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_23288316 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="23288316"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 23288316; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=23288316]").text(description); $(".js-view-count-work_23288316").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_23288316").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="23288316"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="6021" rel="nofollow" href="https://www.academia.edu/Documents/in/Cancer">Cancer</a>, <script data-card-contents-for-ri="6021" type="text/json">{"id":6021,"name":"Cancer","url":"https://www.academia.edu/Documents/in/Cancer?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="105308" rel="nofollow" href="https://www.academia.edu/Documents/in/Renal_cell_Carcinoma">Renal cell Carcinoma</a>, <script data-card-contents-for-ri="105308" type="text/json">{"id":105308,"name":"Renal cell Carcinoma","url":"https://www.academia.edu/Documents/in/Renal_cell_Carcinoma?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="289271" rel="nofollow" href="https://www.academia.edu/Documents/in/Aged">Aged</a><script data-card-contents-for-ri="289271" type="text/json">{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=23288316]'), work: {"id":23288316,"title":"Sunitinib-induced hyperparathyroidism","created_at":"2016-03-15T11:18:20.092-07:00","url":"https://www.academia.edu/23288316/Sunitinib_induced_hyperparathyroidism?f_ri=9534","dom_id":"work_23288316","summary":"BACKGROUND: Sunitinib malate is an orally bioavailable tyrosine kinase inhibitor that is active against many tyrosine kinase receptors involving crucial pathways in both healthy tissues and malignant tissues. Because its use in clinical practice is quite recent, many of its possible side effects remain unknown. In this report, the authors describe the incidence of new-onset hyperparathyroidism in a cohort of patients with metastatic renal cell carcinoma who received treatment with sunitinib. METHODS: Twenty-six patients who received first-line sunitinib for metastatic renal cell carcinoma were enrolled in this study for a mineral and parathyroid function assessment. Plasma levels of intact parathyroid hormone; serum levels of calcium, phosphorus, 25-hydroxyvitamin D 3 , and 1,25-dihydrovitamin D 3 ; and urinary 24-hour calcium and phosphorus excretion all were measured in each patient. Biochemical evaluations were performed before the beginning of treatment and at the end of each sunitinib treatment period. RESULTS: Eighteen of 26 patients (69.2%) developed hyperparathyroidism with normal serum calcium levels, and 6 of them developed hypophosphatemia. Patients presented with a mean elevation of parathyroid hormone after 2.2 cycles of sunitinib. The levels of 25-OH vitamin D 3 were stable over the course of treatment, whereas 1,25-OH vitamin D 3 levels were increased in 5 hyperparathyroid patients. Those who presenting with elevated parathyroid hormone levels had low or undetectable urinary calcium levels. Parathyroid hormone elevation usually persisted but did not progress during long-term therapy with sunitinib. Permanent treatment interruption resulted in a resolution of hyperparathyroidism. CONCLUSIONS: Hyperparathyroidism developed in an high percentage of patients on sunitinib. Therefore, the authors concluded that sunitinib may affect parathyroid function and bone mineral homeostasis, possibly resulting in abnormal bone remodeling. Cancer 2012;118:3165-72","downloadable_attachments":[{"id":43756228,"asset_id":23288316,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":45174342,"first_name":"Alberto","last_name":"Lapini","domain_name":"independent","page_name":"AlbertoLapini","display_name":"Alberto Lapini","profile_url":"https://independent.academia.edu/AlbertoLapini?f_ri=9534","photo":"/images/s65_no_pic.png"},{"id":45206317,"first_name":"Valentina","last_name":"Baldazzi","domain_name":"independent","page_name":"BaldazziValentina","display_name":"Valentina Baldazzi","profile_url":"https://independent.academia.edu/BaldazziValentina?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":6021,"name":"Cancer","url":"https://www.academia.edu/Documents/in/Cancer?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":105308,"name":"Renal cell Carcinoma","url":"https://www.academia.edu/Documents/in/Renal_cell_Carcinoma?f_ri=9534","nofollow":true},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=9534","nofollow":true},{"id":787023,"name":"Hyperparathyroidism","url":"https://www.academia.edu/Documents/in/Hyperparathyroidism?f_ri=9534"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents?f_ri=9534"},{"id":1256683,"name":"Bone and Bones","url":"https://www.academia.edu/Documents/in/Bone_and_Bones?f_ri=9534"},{"id":1343089,"name":"Protein Kinase Inhibitors","url":"https://www.academia.edu/Documents/in/Protein_Kinase_Inhibitors?f_ri=9534"},{"id":2209628,"name":"Hypophosphatemia","url":"https://www.academia.edu/Documents/in/Hypophosphatemia?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15149763 coauthored" data-work_id="15149763" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15149763/Gain_and_loss_of_function_of_P2X7_receptors_mechanisms_pharmacology_and_relevance_to_diabetic_neuropathic_pain">Gain and loss of function of P2X7 receptors: mechanisms, pharmacology and relevance to diabetic neuropathic pain</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: Genetic causes of exaggerated or reduced pain sensitivity in humans are well known. Recently, single nucleotide polymorphisms (SNPs) in the gene P2RX7, coding for the ATP-gated ion channel P2X 7, have been described that cause... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15149763" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: Genetic causes of exaggerated or reduced pain sensitivity in humans are well known. Recently, single nucleotide polymorphisms (SNPs) in the gene P2RX7, coding for the ATP-gated ion channel P2X 7, have been described that cause gain-of-function (GOF) and loss-of-function (LOF), respectively of this channel. Importantly, P2RX7 SNPs have been associated with more or less severe pain scores in patient suffering of post-mastectomy pain and osteoarthritis. Results: The functional consequences of some P2RX7 SNPs (rs208294 (His155Tyr), rs1718119 (Ala348Thr) and rs3751143 (Glu496Ala)) were studied in recombinant cells in vitro. Our findings suggest a correlation between GOF and LOF of P2X 7 and actual channel protein expression. Both channel and pore function for these mutant P2X 7 receptors changed in parallel to protein levels. On the other hand, the mutant receptors did not differ in their sensitivity to known P2X 7 agonists and antagonists. We further demonstrated that in patients with diabetic peripheral neuropathic pain (DPNP), the presence of the GOF SNPs rs208294 (His155Tyr) and rs1718119 (Ala348Thr) is associated, in females, with higher pain intensity scores.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15149763" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="877e297787e25c564b9ae1fb35b37c6c" rel="nofollow" data-download="{"attachment_id":43528982,"asset_id":15149763,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43528982/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34188960" href="https://independent.academia.edu/WeiZou2">Wei Zou</a><script data-card-contents-for-user="34188960" type="text/json">{"id":34188960,"first_name":"Wei","last_name":"Zou","domain_name":"independent","page_name":"WeiZou2","display_name":"Wei Zou","profile_url":"https://independent.academia.edu/WeiZou2?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-15149763">+1</span><div class="hidden js-additional-users-15149763"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/YueweiQian">Yue-wei Qian</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-15149763'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-15149763').html(); 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Recently, single nucleotide polymorphisms (SNPs) in the gene P2RX7, coding for the ATP-gated ion channel P2X 7, have been described that cause gain-of-function (GOF) and loss-of-function (LOF), respectively of this channel. Importantly, P2RX7 SNPs have been associated with more or less severe pain scores in patient suffering of post-mastectomy pain and osteoarthritis. Results: The functional consequences of some P2RX7 SNPs (rs208294 (His155Tyr), rs1718119 (Ala348Thr) and rs3751143 (Glu496Ala)) were studied in recombinant cells in vitro. Our findings suggest a correlation between GOF and LOF of P2X 7 and actual channel protein expression. Both channel and pore function for these mutant P2X 7 receptors changed in parallel to protein levels. On the other hand, the mutant receptors did not differ in their sensitivity to known P2X 7 agonists and antagonists. We further demonstrated that in patients with diabetic peripheral neuropathic pain (DPNP), the presence of the GOF SNPs rs208294 (His155Tyr) and rs1718119 (Ala348Thr) is associated, in females, with higher pain intensity scores.","downloadable_attachments":[{"id":43528982,"asset_id":15149763,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34188960,"first_name":"Wei","last_name":"Zou","domain_name":"independent","page_name":"WeiZou2","display_name":"Wei Zou","profile_url":"https://independent.academia.edu/WeiZou2?f_ri=9534","photo":"/images/s65_no_pic.png"},{"id":34349698,"first_name":"Yue-wei","last_name":"Qian","domain_name":"independent","page_name":"YueweiQian","display_name":"Yue-wei Qian","profile_url":"https://independent.academia.edu/YueweiQian?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype?f_ri=9534","nofollow":true},{"id":413194,"name":"Analysis of Variance","url":"https://www.academia.edu/Documents/in/Analysis_of_Variance?f_ri=9534","nofollow":true},{"id":620070,"name":"Transfection","url":"https://www.academia.edu/Documents/in/Transfection?f_ri=9534","nofollow":true},{"id":824508,"name":"Benzoxazoles","url":"https://www.academia.edu/Documents/in/Benzoxazoles?f_ri=9534"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=9534"},{"id":1262481,"name":"Pain Measurement","url":"https://www.academia.edu/Documents/in/Pain_Measurement?f_ri=9534"},{"id":1763968,"name":"Gene Expression Regulation","url":"https://www.academia.edu/Documents/in/Gene_Expression_Regulation?f_ri=9534"},{"id":1816594,"name":"Adenosine Triphosphate","url":"https://www.academia.edu/Documents/in/Adenosine_Triphosphate?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22569938" data-work_id="22569938" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22569938/Effects_of_Oxidants_on_Properties_of_Fluorescent_Calcium_Indicators">Effects of Oxidants on Properties of Fluorescent Calcium Indicators</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">to know the susceptibility of fluorescent probes to oxi-An increasing number of studies use calcium-sensi-dants. This important issue is being addressed by the tive fluorescent dyes to address the relationship be-present study, which... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_22569938" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">to know the susceptibility of fluorescent probes to oxi-An increasing number of studies use calcium-sensi-dants. This important issue is being addressed by the tive fluorescent dyes to address the relationship be-present study, which evaluates the effects of several tween elevated levels of intracellular calcium and freeoxidant-generating systems on the spectral properties radical-mediated damage in a variety of pathophysioof such commonly used probes as Fluo-3 and Fura-2.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22569938" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="790e33bdf52610a8efc5b1c7e7a11083" rel="nofollow" data-download="{"attachment_id":43179282,"asset_id":22569938,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43179282/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="44119461" href="https://independent.academia.edu/NSarvazyan">N. 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This important issue is being addressed by the tive fluorescent dyes to address the relationship be-present study, which evaluates the effects of several tween elevated levels of intracellular calcium and freeoxidant-generating systems on the spectral properties radical-mediated damage in a variety of pathophysioof such commonly used probes as Fluo-3 and Fura-2.","downloadable_attachments":[{"id":43179282,"asset_id":22569938,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":44119461,"first_name":"N.","last_name":"Sarvazyan","domain_name":"independent","page_name":"NSarvazyan","display_name":"N. Sarvazyan","profile_url":"https://independent.academia.edu/NSarvazyan?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":2425,"name":"Free Radicals","url":"https://www.academia.edu/Documents/in/Free_Radicals?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=9534","nofollow":true},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=9534","nofollow":true},{"id":44022,"name":"Free Radical","url":"https://www.academia.edu/Documents/in/Free_Radical?f_ri=9534"},{"id":55175,"name":"Peroxidase","url":"https://www.academia.edu/Documents/in/Peroxidase?f_ri=9534"},{"id":172678,"name":"Artifacts","url":"https://www.academia.edu/Documents/in/Artifacts?f_ri=9534"},{"id":730649,"name":"Spectral Properties","url":"https://www.academia.edu/Documents/in/Spectral_Properties?f_ri=9534"},{"id":1352490,"name":"Oxidants","url":"https://www.academia.edu/Documents/in/Oxidants?f_ri=9534"},{"id":1599862,"name":"Fura","url":"https://www.academia.edu/Documents/in/Fura?f_ri=9534"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=9534"},{"id":1782947,"name":"aniline Compounds","url":"https://www.academia.edu/Documents/in/aniline_Compounds?f_ri=9534"},{"id":1823382,"name":"Intracellular Calcium","url":"https://www.academia.edu/Documents/in/Intracellular_Calcium?f_ri=9534"},{"id":1956581,"name":"Dynamic Range","url":"https://www.academia.edu/Documents/in/Dynamic_Range?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21975515" data-work_id="21975515" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21975515/Pathways_through_which_glucose_induces_a_rise_in_Ca2_i_of_polymorphonuclear_leukocytes_of_rats">Pathways through which glucose induces a rise in [Ca2+]i of polymorphonuclear leukocytes of rats</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Pathways through which glucose induces a rise in [Ca2]i of polymorphonuclear leukocytes of rats. Basal levels of [Ca2}i arc elevated in diabetes mellitus. Such an abnormality is most likely due to both increased calcium influx into cells... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21975515" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Pathways through which glucose induces a rise in [Ca2]i of polymorphonuclear leukocytes of rats. Basal levels of [Ca2}i arc elevated in diabetes mellitus. Such an abnormality is most likely due to both increased calcium influx into cells and decreased efflux of this ion out of the cells. The present study examined the cellular pathways that are responsible for</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21975515" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bb3e380211bffba9ae249bcf00c52774" rel="nofollow" data-download="{"attachment_id":42688934,"asset_id":21975515,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42688934/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="43234295" href="https://independent.academia.edu/MiroslawSmogorzewski">Miroslaw Smogorzewski</a><script data-card-contents-for-user="43234295" type="text/json">{"id":43234295,"first_name":"Miroslaw","last_name":"Smogorzewski","domain_name":"independent","page_name":"MiroslawSmogorzewski","display_name":"Miroslaw Smogorzewski","profile_url":"https://independent.academia.edu/MiroslawSmogorzewski?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21975515 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21975515"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21975515, container: ".js-paper-rank-work_21975515", }); 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$(".js-view-count[data-work-id=21975515]").text(description); $(".js-view-count-work_21975515").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21975515").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21975515"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="71289" rel="nofollow" href="https://www.academia.edu/Documents/in/Glucose">Glucose</a>, <script data-card-contents-for-ri="71289" type="text/json">{"id":71289,"name":"Glucose","url":"https://www.academia.edu/Documents/in/Glucose?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="71294" rel="nofollow" href="https://www.academia.edu/Documents/in/Kidney">Kidney</a>, <script data-card-contents-for-ri="71294" type="text/json">{"id":71294,"name":"Kidney","url":"https://www.academia.edu/Documents/in/Kidney?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="71511" rel="nofollow" href="https://www.academia.edu/Documents/in/Diabetes_mellitus">Diabetes mellitus</a><script data-card-contents-for-ri="71511" type="text/json">{"id":71511,"name":"Diabetes mellitus","url":"https://www.academia.edu/Documents/in/Diabetes_mellitus?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21975515]'), work: {"id":21975515,"title":"Pathways through which glucose induces a rise in [Ca2+]i of polymorphonuclear leukocytes of rats","created_at":"2016-02-14T22:17:29.350-08:00","url":"https://www.academia.edu/21975515/Pathways_through_which_glucose_induces_a_rise_in_Ca2_i_of_polymorphonuclear_leukocytes_of_rats?f_ri=9534","dom_id":"work_21975515","summary":"Pathways through which glucose induces a rise in [Ca2]i of polymorphonuclear leukocytes of rats. Basal levels of [Ca2}i arc elevated in diabetes mellitus. Such an abnormality is most likely due to both increased calcium influx into cells and decreased efflux of this ion out of the cells. The present study examined the cellular pathways that are responsible for","downloadable_attachments":[{"id":42688934,"asset_id":21975515,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":43234295,"first_name":"Miroslaw","last_name":"Smogorzewski","domain_name":"independent","page_name":"MiroslawSmogorzewski","display_name":"Miroslaw Smogorzewski","profile_url":"https://independent.academia.edu/MiroslawSmogorzewski?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":71289,"name":"Glucose","url":"https://www.academia.edu/Documents/in/Glucose?f_ri=9534","nofollow":true},{"id":71294,"name":"Kidney","url":"https://www.academia.edu/Documents/in/Kidney?f_ri=9534","nofollow":true},{"id":71511,"name":"Diabetes mellitus","url":"https://www.academia.edu/Documents/in/Diabetes_mellitus?f_ri=9534","nofollow":true},{"id":92680,"name":"cyclic AMP","url":"https://www.academia.edu/Documents/in/cyclic_AMP?f_ri=9534"},{"id":140122,"name":"Calcium Channel","url":"https://www.academia.edu/Documents/in/Calcium_Channel?f_ri=9534"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":295260,"name":"Neutrophils","url":"https://www.academia.edu/Documents/in/Neutrophils?f_ri=9534"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=9534"},{"id":394477,"name":"Time Dependent","url":"https://www.academia.edu/Documents/in/Time_Dependent?f_ri=9534"},{"id":780543,"name":"G protein","url":"https://www.academia.edu/Documents/in/G_protein?f_ri=9534"},{"id":790002,"name":"Streptozotocin","url":"https://www.academia.edu/Documents/in/Streptozotocin?f_ri=9534"},{"id":1242620,"name":"Cell Volume","url":"https://www.academia.edu/Documents/in/Cell_Volume?f_ri=9534"},{"id":1288743,"name":"Gtp Binding Proteins","url":"https://www.academia.edu/Documents/in/Gtp_Binding_Proteins?f_ri=9534"},{"id":2225479,"name":"Phospholipase C","url":"https://www.academia.edu/Documents/in/Phospholipase_C?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16569583 coauthored" data-work_id="16569583" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16569583/Temporospatial_coupling_of_networked_synaptic_activation_of_AMPA_type_glutamate_receptor_channels_and_calcium_transients_in_cultured_motoneurons">Temporospatial coupling of networked synaptic activation of AMPA-type glutamate receptor channels and calcium transients in cultured motoneurons</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">AMPA-type glutamate receptor (GluR) channels provide fast excitatory synaptic transmission in the CNS, but mediate also cytotoxic insults. It could be shown that AMPAtype GluR channel-mediated chronic excitotoxicity leads to an increased... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16569583" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">AMPA-type glutamate receptor (GluR) channels provide fast excitatory synaptic transmission in the CNS, but mediate also cytotoxic insults. It could be shown that AMPAtype GluR channel-mediated chronic excitotoxicity leads to an increased intracellular calcium concentration and plays an important role in neurodegenerative diseases like for example amyotrophic lateral sclerosis (ALS). As calcium is an important mediator of various processes in the cell and calcium signals have to be very precise in the temporospatial resolution, excessive intracellular calcium increases can seriously impair cell function. It is still unclear if AMPA-type receptors can directly interact with the intracellular calcium homeostasis or if other mechanisms are involved in this process. The objective of this study was therefore to investigate the calcium homeostasis in rat motoneurons under physiological stimulation of AMPA-type GluR channels using calcium imaging techniques and patch-clamp recordings simultaneously. It was found that spontaneous excitatory postsynaptic currents of cultured motoneurons did not elicit significant intracellular calcium transients. Large intracellular calcium transients occurred only when preceding fast sodium currents were observed. Pharmacological experiments showed that activation of AMPA-type GluR channels during synaptic transmission has a great functional impact on the calcium homeostasis in motoneurons as all kinds of activity was completely blocked by application of the selective kainateand AMPA-type GluR channel blocker 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Furthermore we suggest from our experiments that calcium transients of several hundred milliseconds' duration result from release of calcium from the endoplasmic reticulum via activation of ryanodine receptors (calcium-induced calcium release, CICR). Our results help to understand the regulatory function of AMPA-type GluR channels in the intracellular calcium homeostasis which is known to be disturbed in neurodegenerative diseases.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16569583" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="eafa2a07becc289fa63b901a72237169" rel="nofollow" data-download="{"attachment_id":42434567,"asset_id":16569583,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42434567/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="63278370" href="https://independent.academia.edu/JahnK">K. Jahn</a><script data-card-contents-for-user="63278370" type="text/json">{"id":63278370,"first_name":"K.","last_name":"Jahn","domain_name":"independent","page_name":"JahnK","display_name":"K. Jahn","profile_url":"https://independent.academia.edu/JahnK?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-16569583">+1</span><div class="hidden js-additional-users-16569583"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://uniklinikum-jena.academia.edu/JulianGrosskreutz">Julian Grosskreutz</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-16569583'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-16569583').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_16569583 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16569583"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 16569583, container: ".js-paper-rank-work_16569583", }); });</script></li><li class="js-percentile-work_16569583 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16569583; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_16569583"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_16569583 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="16569583"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16569583; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16569583]").text(description); $(".js-view-count-work_16569583").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_16569583").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16569583"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">32</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="161" rel="nofollow" href="https://www.academia.edu/Documents/in/Neuroscience">Neuroscience</a>, <script data-card-contents-for-ri="161" type="text/json">{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="221" rel="nofollow" href="https://www.academia.edu/Documents/in/Psychology">Psychology</a>, <script data-card-contents-for-ri="221" type="text/json">{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2007" rel="nofollow" href="https://www.academia.edu/Documents/in/Electrophysiology">Electrophysiology</a>, <script data-card-contents-for-ri="2007" type="text/json">{"id":2007,"name":"Electrophysiology","url":"https://www.academia.edu/Documents/in/Electrophysiology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6201" rel="nofollow" href="https://www.academia.edu/Documents/in/Imaging">Imaging</a><script data-card-contents-for-ri="6201" type="text/json">{"id":6201,"name":"Imaging","url":"https://www.academia.edu/Documents/in/Imaging?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16569583]'), work: {"id":16569583,"title":"Temporospatial coupling of networked synaptic activation of AMPA-type glutamate receptor channels and calcium transients in cultured motoneurons","created_at":"2015-10-08T07:56:42.583-07:00","url":"https://www.academia.edu/16569583/Temporospatial_coupling_of_networked_synaptic_activation_of_AMPA_type_glutamate_receptor_channels_and_calcium_transients_in_cultured_motoneurons?f_ri=9534","dom_id":"work_16569583","summary":"AMPA-type glutamate receptor (GluR) channels provide fast excitatory synaptic transmission in the CNS, but mediate also cytotoxic insults. It could be shown that AMPAtype GluR channel-mediated chronic excitotoxicity leads to an increased intracellular calcium concentration and plays an important role in neurodegenerative diseases like for example amyotrophic lateral sclerosis (ALS). As calcium is an important mediator of various processes in the cell and calcium signals have to be very precise in the temporospatial resolution, excessive intracellular calcium increases can seriously impair cell function. It is still unclear if AMPA-type receptors can directly interact with the intracellular calcium homeostasis or if other mechanisms are involved in this process. The objective of this study was therefore to investigate the calcium homeostasis in rat motoneurons under physiological stimulation of AMPA-type GluR channels using calcium imaging techniques and patch-clamp recordings simultaneously. It was found that spontaneous excitatory postsynaptic currents of cultured motoneurons did not elicit significant intracellular calcium transients. Large intracellular calcium transients occurred only when preceding fast sodium currents were observed. Pharmacological experiments showed that activation of AMPA-type GluR channels during synaptic transmission has a great functional impact on the calcium homeostasis in motoneurons as all kinds of activity was completely blocked by application of the selective kainateand AMPA-type GluR channel blocker 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Furthermore we suggest from our experiments that calcium transients of several hundred milliseconds' duration result from release of calcium from the endoplasmic reticulum via activation of ryanodine receptors (calcium-induced calcium release, CICR). Our results help to understand the regulatory function of AMPA-type GluR channels in the intracellular calcium homeostasis which is known to be disturbed in neurodegenerative diseases.","downloadable_attachments":[{"id":42434567,"asset_id":16569583,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":63278370,"first_name":"K.","last_name":"Jahn","domain_name":"independent","page_name":"JahnK","display_name":"K. Jahn","profile_url":"https://independent.academia.edu/JahnK?f_ri=9534","photo":"/images/s65_no_pic.png"},{"id":35875580,"first_name":"Julian","last_name":"Grosskreutz","domain_name":"uniklinikum-jena","page_name":"JulianGrosskreutz","display_name":"Julian Grosskreutz","profile_url":"https://uniklinikum-jena.academia.edu/JulianGrosskreutz?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=9534","nofollow":true},{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=9534","nofollow":true},{"id":2007,"name":"Electrophysiology","url":"https://www.academia.edu/Documents/in/Electrophysiology?f_ri=9534","nofollow":true},{"id":6201,"name":"Imaging","url":"https://www.academia.edu/Documents/in/Imaging?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534"},{"id":37793,"name":"Patch-clamp and imaging techniques","url":"https://www.academia.edu/Documents/in/Patch-clamp_and_imaging_techniques?f_ri=9534"},{"id":42973,"name":"Amyotrophic Lateral Sclerosis","url":"https://www.academia.edu/Documents/in/Amyotrophic_Lateral_Sclerosis?f_ri=9534"},{"id":64467,"name":"Calcium Imaging","url":"https://www.academia.edu/Documents/in/Calcium_Imaging?f_ri=9534"},{"id":67405,"name":"Toxicity","url":"https://www.academia.edu/Documents/in/Toxicity?f_ri=9534"},{"id":99271,"name":"Calcium channels","url":"https://www.academia.edu/Documents/in/Calcium_channels?f_ri=9534"},{"id":99421,"name":"Spinal Cord","url":"https://www.academia.edu/Documents/in/Spinal_Cord?f_ri=9534"},{"id":104808,"name":"Roi","url":"https://www.academia.edu/Documents/in/Roi?f_ri=9534"},{"id":122187,"name":"Endoplasmic Reticulum","url":"https://www.academia.edu/Documents/in/Endoplasmic_Reticulum?f_ri=9534"},{"id":140081,"name":"Calcium Signaling","url":"https://www.academia.edu/Documents/in/Calcium_Signaling?f_ri=9534"},{"id":140122,"name":"Calcium Channel","url":"https://www.academia.edu/Documents/in/Calcium_Channel?f_ri=9534"},{"id":146052,"name":"Ryanodine Receptor","url":"https://www.academia.edu/Documents/in/Ryanodine_Receptor?f_ri=9534"},{"id":176503,"name":"Synaptic Transmission","url":"https://www.academia.edu/Documents/in/Synaptic_Transmission?f_ri=9534"},{"id":239480,"name":"Neurotoxins","url":"https://www.academia.edu/Documents/in/Neurotoxins?f_ri=9534"},{"id":251027,"name":"NMDA","url":"https://www.academia.edu/Documents/in/NMDA?f_ri=9534"},{"id":293675,"name":"Calcium Homeostasis","url":"https://www.academia.edu/Documents/in/Calcium_Homeostasis?f_ri=9534"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=9534"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=9534"},{"id":470846,"name":"Voltage-Gated Sodium Channels","url":"https://www.academia.edu/Documents/in/Voltage-Gated_Sodium_Channels?f_ri=9534"},{"id":527780,"name":"Neurodegenerative Disease","url":"https://www.academia.edu/Documents/in/Neurodegenerative_Disease?f_ri=9534"},{"id":666669,"name":"Action potential","url":"https://www.academia.edu/Documents/in/Action_potential?f_ri=9534"},{"id":704401,"name":"Neural pathways","url":"https://www.academia.edu/Documents/in/Neural_pathways?f_ri=9534"},{"id":955727,"name":"Action Potentials","url":"https://www.academia.edu/Documents/in/Action_Potentials?f_ri=9534"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=9534"},{"id":1292998,"name":"Glutamic Acid","url":"https://www.academia.edu/Documents/in/Glutamic_Acid?f_ri=9534"},{"id":1592440,"name":"Patch Clamp","url":"https://www.academia.edu/Documents/in/Patch_Clamp?f_ri=9534"},{"id":1823382,"name":"Intracellular Calcium","url":"https://www.academia.edu/Documents/in/Intracellular_Calcium?f_ri=9534"},{"id":2012816,"name":"Glutamate Receptor","url":"https://www.academia.edu/Documents/in/Glutamate_Receptor?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19323769" data-work_id="19323769" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19323769/Evidence_that_TRPC1_contributes_to_calcium_induced_differentiation_of_human_keratinocytes">Evidence that TRPC1 contributes to calcium-induced differentiation of human keratinocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">External calcium ion concentration is a major regulator of epidermal keratinocyte differentiation in vitro and probably also in vivo. Regulation of calcium-induced differentiation changes is proposed to occur via an external... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19323769" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">External calcium ion concentration is a major regulator of epidermal keratinocyte differentiation in vitro and probably also in vivo. Regulation of calcium-induced differentiation changes is proposed to occur via an external calcium-sensing, signaling pathway that utilizes increases in intracellular calcium ion concentration to activate differentiation-related gene expression. Calcium ion release from intracellular stores and calcium ion influx via storeoperated calcium-permeable channels are key elements in this proposed signaling pathway; however, the channels involved have not yet been identified. The present report shows that human gingival keratinocytes (HGKs) also undergo calcium-induced differentiation in vitro as indicated by involucrin expression and morphological changes. Moreover, TRPC1, which functions as a store-operated calcium channel in a number of cell types, including epidermal keratinocytes, is expressed in both proliferating and differentiating HGKs. Transfection of HGKs with TRPC1 siRNA disrupted expression of TRPC1 mRNA and protein com-pared with transfection with scrambled TRPC1 siRNA. Cells with disrupted TRPC1 expression showed decreased calcium-induced differentiation as measured by involucrin expression or morphological changes, as well as decreased thapsigargin-induced calcium ion influx, and a decreased rate of store calcium release. These results indicate that TRPC1 is involved in calcium-induced differentiation of HGKs likely by supporting a store-operated calcium ion influx.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19323769" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="197f2406c9591fde8bcf19681e9e3f71" rel="nofollow" data-download="{"attachment_id":40556272,"asset_id":19323769,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40556272/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39578568" href="https://washington.academia.edu/RichardPresland">Richard Presland</a><script data-card-contents-for-user="39578568" type="text/json">{"id":39578568,"first_name":"Richard","last_name":"Presland","domain_name":"washington","page_name":"RichardPresland","display_name":"Richard Presland","profile_url":"https://washington.academia.edu/RichardPresland?f_ri=9534","photo":"https://0.academia-photos.com/39578568/12757414/14188918/s65_richard.presland.jpg"}</script></span></span></li><li class="js-paper-rank-work_19323769 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19323769"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19323769, container: ".js-paper-rank-work_19323769", }); });</script></li><li class="js-percentile-work_19323769 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 19323769; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_19323769"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_19323769 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="19323769"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 19323769; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=19323769]").text(description); $(".js-view-count-work_19323769").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19323769").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19323769"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="167" rel="nofollow" href="https://www.academia.edu/Documents/in/Physiology">Physiology</a>, <script data-card-contents-for-ri="167" type="text/json">{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="27784" rel="nofollow" href="https://www.academia.edu/Documents/in/Gene_expression">Gene expression</a>, <script data-card-contents-for-ri="27784" type="text/json">{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="51121" rel="nofollow" href="https://www.academia.edu/Documents/in/RNA_interference">RNA interference</a><script data-card-contents-for-ri="51121" type="text/json">{"id":51121,"name":"RNA interference","url":"https://www.academia.edu/Documents/in/RNA_interference?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19323769]'), work: {"id":19323769,"title":"Evidence that TRPC1 contributes to calcium-induced differentiation of human keratinocytes","created_at":"2015-12-01T15:07:00.725-08:00","url":"https://www.academia.edu/19323769/Evidence_that_TRPC1_contributes_to_calcium_induced_differentiation_of_human_keratinocytes?f_ri=9534","dom_id":"work_19323769","summary":"External calcium ion concentration is a major regulator of epidermal keratinocyte differentiation in vitro and probably also in vivo. Regulation of calcium-induced differentiation changes is proposed to occur via an external calcium-sensing, signaling pathway that utilizes increases in intracellular calcium ion concentration to activate differentiation-related gene expression. Calcium ion release from intracellular stores and calcium ion influx via storeoperated calcium-permeable channels are key elements in this proposed signaling pathway; however, the channels involved have not yet been identified. The present report shows that human gingival keratinocytes (HGKs) also undergo calcium-induced differentiation in vitro as indicated by involucrin expression and morphological changes. Moreover, TRPC1, which functions as a store-operated calcium channel in a number of cell types, including epidermal keratinocytes, is expressed in both proliferating and differentiating HGKs. Transfection of HGKs with TRPC1 siRNA disrupted expression of TRPC1 mRNA and protein com-pared with transfection with scrambled TRPC1 siRNA. Cells with disrupted TRPC1 expression showed decreased calcium-induced differentiation as measured by involucrin expression or morphological changes, as well as decreased thapsigargin-induced calcium ion influx, and a decreased rate of store calcium release. These results indicate that TRPC1 is involved in calcium-induced differentiation of HGKs likely by supporting a store-operated calcium ion influx.","downloadable_attachments":[{"id":40556272,"asset_id":19323769,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39578568,"first_name":"Richard","last_name":"Presland","domain_name":"washington","page_name":"RichardPresland","display_name":"Richard Presland","profile_url":"https://washington.academia.edu/RichardPresland?f_ri=9534","photo":"https://0.academia-photos.com/39578568/12757414/14188918/s65_richard.presland.jpg"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression?f_ri=9534","nofollow":true},{"id":51121,"name":"RNA interference","url":"https://www.academia.edu/Documents/in/RNA_interference?f_ri=9534","nofollow":true},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation?f_ri=9534"},{"id":70552,"name":"Keratinocytes","url":"https://www.academia.edu/Documents/in/Keratinocytes?f_ri=9534"},{"id":140122,"name":"Calcium Channel","url":"https://www.academia.edu/Documents/in/Calcium_Channel?f_ri=9534"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=9534"},{"id":279027,"name":"European","url":"https://www.academia.edu/Documents/in/European?f_ri=9534"},{"id":782251,"name":"Cell Proliferation","url":"https://www.academia.edu/Documents/in/Cell_Proliferation?f_ri=9534"},{"id":1823382,"name":"Intracellular Calcium","url":"https://www.academia.edu/Documents/in/Intracellular_Calcium?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_28299392 coauthored" data-work_id="28299392" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/28299392/Dynamics_of_mitochondrial_Ca2_measured_with_the_low_Ca2_affinity_dye_rhod_5N">Dynamics of mitochondrial [Ca2+] measured with the low-Ca2+-affinity dye rhod-5N</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Available methods to measure mitochondrial [Ca 2+ ] ([Ca 2+ ] M ) include both targeted proteins and fluorescent dyes. Targeted proteins usually report much higher [Ca 2+ ] M values than fluorescent dyes, up to two orders of magnitude.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_28299392" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Available methods to measure mitochondrial [Ca 2+ ] ([Ca 2+ ] M ) include both targeted proteins and fluorescent dyes. Targeted proteins usually report much higher [Ca 2+ ] M values than fluorescent dyes, up to two orders of magnitude. However, we show here that the low-Ca 2+ -affinity dye rhod-5N provides [Ca 2+ ] M values similar to those reported by targeted aequorin, suggesting that the discrepancies are mainly due to the higher Ca 2+ -affinity of the fluorescent dyes used. We find rhod-5N has an apparent in situ intramitochondrial Kd around 0.5 mM. Addition of Ca 2+ buffers containing between 4.5 and 10 M [Ca 2+ ] to permeabilized cells loaded with rhod-5N induced increases in calibrated [Ca 2+ ] M up to the 100 M-1 mM range, which were dependent on mitochondrial membrane potential. Ca 2+ release from mitochondria was largely dependent on [Na + ]. We have then used rhod-5N loaded cells to investigate the [Ca 2+ ] M response to agonist stimulation at the single-cell and subcellular level.The [Ca 2+ ] M peaks induced by histamine varied by nearly 10-fold among different cells, with a mean about 25 M. In the presence of the Ca 2+ uniporter stimulator kaempferol, the [Ca 2+ ] M peaks induced by histamine were also highly variable, and the mean [Ca 2+ ] M peak was 3-fold higher. Simultaneous measurement of cytosolic and mitochondrial [Ca 2+ ] peaks showed little correlation among the heights of the peaks in both compartments. Studying the [Ca 2+ ] M peaks at the subcellular level, we found significant heterogeneities among regions in the same cell. In particular, the [Ca 2+ ] M increase in mitochondrial regions close to the nucleus was more than double that of mitochondrial regions far from the nucleus.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/28299392" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7409438d8feb6686d9fcbc46ea497974" rel="nofollow" data-download="{"attachment_id":48632317,"asset_id":28299392,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48632317/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="53068782" href="https://uanl.academia.edu/SergiodelaFuente">Sergio de la Fuente</a><script data-card-contents-for-user="53068782" type="text/json">{"id":53068782,"first_name":"Sergio de la","last_name":"Fuente","domain_name":"uanl","page_name":"SergiodelaFuente","display_name":"Sergio de la Fuente","profile_url":"https://uanl.academia.edu/SergiodelaFuente?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-28299392">+1</span><div class="hidden js-additional-users-28299392"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://uva-es.academia.edu/RosalbaFonteriz">Rosalba Fonteriz</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-28299392'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-28299392').html(); 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Targeted proteins usually report much higher [Ca 2+ ] M values than fluorescent dyes, up to two orders of magnitude. However, we show here that the low-Ca 2+ -affinity dye rhod-5N provides [Ca 2+ ] M values similar to those reported by targeted aequorin, suggesting that the discrepancies are mainly due to the higher Ca 2+ -affinity of the fluorescent dyes used. We find rhod-5N has an apparent in situ intramitochondrial Kd around 0.5 mM. Addition of Ca 2+ buffers containing between 4.5 and 10 M [Ca 2+ ] to permeabilized cells loaded with rhod-5N induced increases in calibrated [Ca 2+ ] M up to the 100 M-1 mM range, which were dependent on mitochondrial membrane potential. Ca 2+ release from mitochondria was largely dependent on [Na + ]. We have then used rhod-5N loaded cells to investigate the [Ca 2+ ] M response to agonist stimulation at the single-cell and subcellular level.The [Ca 2+ ] M peaks induced by histamine varied by nearly 10-fold among different cells, with a mean about 25 M. In the presence of the Ca 2+ uniporter stimulator kaempferol, the [Ca 2+ ] M peaks induced by histamine were also highly variable, and the mean [Ca 2+ ] M peak was 3-fold higher. Simultaneous measurement of cytosolic and mitochondrial [Ca 2+ ] peaks showed little correlation among the heights of the peaks in both compartments. Studying the [Ca 2+ ] M peaks at the subcellular level, we found significant heterogeneities among regions in the same cell. In particular, the [Ca 2+ ] M increase in mitochondrial regions close to the nucleus was more than double that of mitochondrial regions far from the nucleus.","downloadable_attachments":[{"id":48632317,"asset_id":28299392,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":53068782,"first_name":"Sergio de la","last_name":"Fuente","domain_name":"uanl","page_name":"SergiodelaFuente","display_name":"Sergio de la Fuente","profile_url":"https://uanl.academia.edu/SergiodelaFuente?f_ri=9534","photo":"/images/s65_no_pic.png"},{"id":32528603,"first_name":"Rosalba","last_name":"Fonteriz","domain_name":"uva-es","page_name":"RosalbaFonteriz","display_name":"Rosalba Fonteriz","profile_url":"https://uva-es.academia.edu/RosalbaFonteriz?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":15719,"name":"Mitochondria","url":"https://www.academia.edu/Documents/in/Mitochondria?f_ri=9534","nofollow":true},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=9534","nofollow":true},{"id":51569,"name":"Histamine","url":"https://www.academia.edu/Documents/in/Histamine?f_ri=9534"},{"id":96893,"name":"Calibration","url":"https://www.academia.edu/Documents/in/Calibration?f_ri=9534"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=9534"},{"id":422325,"name":"HeLa cells","url":"https://www.academia.edu/Documents/in/HeLa_cells?f_ri=9534"},{"id":1311259,"name":"Intracellular Space","url":"https://www.academia.edu/Documents/in/Intracellular_Space?f_ri=9534"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_17989187" data-work_id="17989187" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17989187/Alterations_in_magnesium_and_oxidative_status_during_chronic_emotional_stress">Alterations in magnesium and oxidative status during chronic emotional stress</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Magnesium and oxidative status were investigated in young volunteers exposed to chronic stress (political intolerance, awareness of potential military attacks, permanent stand-by duty and reduced holidays more than 10 years) or subchronic... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_17989187" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Magnesium and oxidative status were investigated in young volunteers exposed to chronic stress (political intolerance, awareness of potential military attacks, permanent stand-by duty and reduced holidays more than 10 years) or subchronic stress consisting of everyday mortal danger in military actions lasting more than 3 months. Significant decreases in plasma ionized Mg2+, total Mg and ionized Ca2+ concentrations were found in both groups. Similarly, both study groups exhibited oxidative stress as assessed by increased plasma superoxide anions and malondialdehyde and modified antioxidant defense. There were no significant differences between the two stress groups. A negative correlation between magnesium balance and oxidative stress was observed suggesting that the same etiological factor (chronic stress) initiate decreases in both free and total magnesium concentrations and simultaneously increase oxidative stress intensity. These findings support the need for magnesium supplement...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17989187" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="44496497244f57b42ab16d545160b2aa" rel="nofollow" data-download="{"attachment_id":39816284,"asset_id":17989187,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/39816284/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="3219392" href="https://bg.academia.edu/MilicVeljovic">Milic Veljovic</a><script data-card-contents-for-user="3219392" type="text/json">{"id":3219392,"first_name":"Milic","last_name":"Veljovic","domain_name":"bg","page_name":"MilicVeljovic","display_name":"Milic Veljovic","profile_url":"https://bg.academia.edu/MilicVeljovic?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_17989187 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="17989187"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 17989187, container: ".js-paper-rank-work_17989187", }); 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$(".js-view-count[data-work-id=17989187]").text(description); $(".js-view-count-work_17989187").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_17989187").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="17989187"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="14292" rel="nofollow" href="https://www.academia.edu/Documents/in/Oxidative_Stress">Oxidative Stress</a>, <script data-card-contents-for-ri="14292" type="text/json">{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" rel="nofollow" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a>, <script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="70902" rel="nofollow" href="https://www.academia.edu/Documents/in/Magnesium">Magnesium</a><script data-card-contents-for-ri="70902" type="text/json">{"id":70902,"name":"Magnesium","url":"https://www.academia.edu/Documents/in/Magnesium?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=17989187]'), work: {"id":17989187,"title":"Alterations in magnesium and oxidative status during chronic emotional stress","created_at":"2015-11-08T22:29:29.217-08:00","url":"https://www.academia.edu/17989187/Alterations_in_magnesium_and_oxidative_status_during_chronic_emotional_stress?f_ri=9534","dom_id":"work_17989187","summary":"Magnesium and oxidative status were investigated in young volunteers exposed to chronic stress (political intolerance, awareness of potential military attacks, permanent stand-by duty and reduced holidays more than 10 years) or subchronic stress consisting of everyday mortal danger in military actions lasting more than 3 months. Significant decreases in plasma ionized Mg2+, total Mg and ionized Ca2+ concentrations were found in both groups. Similarly, both study groups exhibited oxidative stress as assessed by increased plasma superoxide anions and malondialdehyde and modified antioxidant defense. There were no significant differences between the two stress groups. A negative correlation between magnesium balance and oxidative stress was observed suggesting that the same etiological factor (chronic stress) initiate decreases in both free and total magnesium concentrations and simultaneously increase oxidative stress intensity. These findings support the need for magnesium supplement...","downloadable_attachments":[{"id":39816284,"asset_id":17989187,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":3219392,"first_name":"Milic","last_name":"Veljovic","domain_name":"bg","page_name":"MilicVeljovic","display_name":"Milic Veljovic","profile_url":"https://bg.academia.edu/MilicVeljovic?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":14292,"name":"Oxidative Stress","url":"https://www.academia.edu/Documents/in/Oxidative_Stress?f_ri=9534","nofollow":true},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=9534","nofollow":true},{"id":70902,"name":"Magnesium","url":"https://www.academia.edu/Documents/in/Magnesium?f_ri=9534","nofollow":true},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=9534"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=9534"},{"id":1455811,"name":"Superoxides","url":"https://www.academia.edu/Documents/in/Superoxides?f_ri=9534"},{"id":1819399,"name":"Case Control Studies","url":"https://www.academia.edu/Documents/in/Case_Control_Studies?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62762066" data-work_id="62762066" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62762066/Hydrogen_Atoms_Are_Produced_When_Tryptophan_within_a_Protein_Is_Irradiated_with_Ultraviolet_Light">Hydrogen Atoms Are Produced When Tryptophan within a Protein Is Irradiated with Ultraviolet Light</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The UV photolysis of the aromatic amino acid, tryptophan (Trp), in the Ca2+-binding protein, cod parvalbumin, type 111, was studied using electron paramagnetic resonance (EPR) spectroscopy in the temperature range 4-80 K. For the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_62762066" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The UV photolysis of the aromatic amino acid, tryptophan (Trp), in the Ca2+-binding protein, cod parvalbumin, type 111, was studied using electron paramagnetic resonance (EPR) spectroscopy in the temperature range 4-80 K. For the Ca2+-bound protein, irradiation with UV light (250-400 nm) resulted in the generation of atomic hydrogen with a hyperfine splitting of 50.9 mT, whereas in the Ca2+-free form, where the Trp is exposed to solvent, the trapped atomic hydrogen was not in evidence. In the same spectra, the radical signal in the g = 2.00 region could be detected. The line shape of the Ca2+bound form is similar to the EPR line shape obtained for Trp in micellar systems. In contrast, the EPR line shape for the Ca2+-free form is essentially featureless up to 80 K. The EPR spectra of the photoproducts of Trp and the nature of the photoreactions are therefore sensitive to the environment of Trp within the protein.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62762066" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a4a999bff01ec722d8755f1377cf5a4d" rel="nofollow" data-download="{"attachment_id":75428841,"asset_id":62762066,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75428841/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="207999199" href="https://independent.academia.edu/VanderkooiJane">Jane Vanderkooi</a><script data-card-contents-for-user="207999199" type="text/json">{"id":207999199,"first_name":"Jane","last_name":"Vanderkooi","domain_name":"independent","page_name":"VanderkooiJane","display_name":"Jane Vanderkooi","profile_url":"https://independent.academia.edu/VanderkooiJane?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_62762066 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="62762066"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 62762066, container: ".js-paper-rank-work_62762066", }); });</script></li><li class="js-percentile-work_62762066 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 62762066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_62762066"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_62762066 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="62762066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 62762066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=62762066]").text(description); $(".js-view-count-work_62762066").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_62762066").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="62762066"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3771" rel="nofollow" href="https://www.academia.edu/Documents/in/Hydrogen">Hydrogen</a>, <script data-card-contents-for-ri="3771" type="text/json">{"id":3771,"name":"Hydrogen","url":"https://www.academia.edu/Documents/in/Hydrogen?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="117270" rel="nofollow" href="https://www.academia.edu/Documents/in/Fishes">Fishes</a><script data-card-contents-for-ri="117270" type="text/json">{"id":117270,"name":"Fishes","url":"https://www.academia.edu/Documents/in/Fishes?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=62762066]'), work: {"id":62762066,"title":"Hydrogen Atoms Are Produced When Tryptophan within a Protein Is Irradiated with Ultraviolet Light","created_at":"2021-11-30T15:20:26.803-08:00","url":"https://www.academia.edu/62762066/Hydrogen_Atoms_Are_Produced_When_Tryptophan_within_a_Protein_Is_Irradiated_with_Ultraviolet_Light?f_ri=9534","dom_id":"work_62762066","summary":"The UV photolysis of the aromatic amino acid, tryptophan (Trp), in the Ca2+-binding protein, cod parvalbumin, type 111, was studied using electron paramagnetic resonance (EPR) spectroscopy in the temperature range 4-80 K. For the Ca2+-bound protein, irradiation with UV light (250-400 nm) resulted in the generation of atomic hydrogen with a hyperfine splitting of 50.9 mT, whereas in the Ca2+-free form, where the Trp is exposed to solvent, the trapped atomic hydrogen was not in evidence. In the same spectra, the radical signal in the g = 2.00 region could be detected. The line shape of the Ca2+bound form is similar to the EPR line shape obtained for Trp in micellar systems. In contrast, the EPR line shape for the Ca2+-free form is essentially featureless up to 80 K. The EPR spectra of the photoproducts of Trp and the nature of the photoreactions are therefore sensitive to the environment of Trp within the protein.","downloadable_attachments":[{"id":75428841,"asset_id":62762066,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":207999199,"first_name":"Jane","last_name":"Vanderkooi","domain_name":"independent","page_name":"VanderkooiJane","display_name":"Jane Vanderkooi","profile_url":"https://independent.academia.edu/VanderkooiJane?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3771,"name":"Hydrogen","url":"https://www.academia.edu/Documents/in/Hydrogen?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true},{"id":117270,"name":"Fishes","url":"https://www.academia.edu/Documents/in/Fishes?f_ri=9534","nofollow":true},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=9534"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=9534"},{"id":202225,"name":"Tryptophan","url":"https://www.academia.edu/Documents/in/Tryptophan?f_ri=9534"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=9534"},{"id":377108,"name":"Photochemistry and Photobiology","url":"https://www.academia.edu/Documents/in/Photochemistry_and_Photobiology?f_ri=9534"},{"id":623612,"name":"Photolysis","url":"https://www.academia.edu/Documents/in/Photolysis?f_ri=9534"},{"id":818653,"name":"Hydrogen Atom","url":"https://www.academia.edu/Documents/in/Hydrogen_Atom?f_ri=9534"},{"id":3005391,"name":"Ultraviolet Rays","url":"https://www.academia.edu/Documents/in/Ultraviolet_Rays?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_61803103" data-work_id="61803103" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/61803103/Encapsulation_of_fibroblasts_causes_accelerated_alginate_hydrogel_degradation">Encapsulation of fibroblasts causes accelerated alginate hydrogel degradation</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Calcium-alginate hydrogel has been widely studied as a material for cell encapsulation for tissue engineering. At present, the effect that cells have on the degradation of alginate hydrogel is largely unknown. We have shown that... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_61803103" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Calcium-alginate hydrogel has been widely studied as a material for cell encapsulation for tissue engineering. At present, the effect that cells have on the degradation of alginate hydrogel is largely unknown. We have shown that fibroblasts encapsulated at a density of 7.5 Â 10 5 cells ml À1 in both 2% and 5% w/v alginate remain viable for at least 60 days. Rheological analysis was used to study how the mechanical properties exhibited by alginate hydrogel changed during 28 days in vitro culture. Alginate degradation was shown to occur throughout the study but was greatest within the first 7 days of culture for all samples, which correlated with a sharp release of calcium ions from the construct. Fibroblasts were shown to increase the rate of degradation during the first 7 days when compared with acellular samples in both 2% and 5% w/v gels, but after 28 days both acellular and cell-encapsulating samples retained disc-shaped morphologies and gel-like spectra. The results demonstrate that although at an early stage cells influence the mechanical properties of encapsulating alginate, over a longer period of culture, the hydrogels retain sufficient mechanical integrity to exhibit gel-like properties. This allows sustained immobilization of the cells at the desired location in vivo where they can produce extracellular matrix and growth factors to expedite the healing process.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/61803103" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="56a96f3018a5b0a9e45df50a80f79dc2" rel="nofollow" data-download="{"attachment_id":74747460,"asset_id":61803103,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/74747460/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50938277" href="https://independent.academia.edu/NicolaHunt1">Nicola Hunt</a><script data-card-contents-for-user="50938277" type="text/json">{"id":50938277,"first_name":"Nicola","last_name":"Hunt","domain_name":"independent","page_name":"NicolaHunt1","display_name":"Nicola Hunt","profile_url":"https://independent.academia.edu/NicolaHunt1?f_ri=9534","photo":"https://0.academia-photos.com/50938277/62919797/51218268/s65_nicola.hunt.png"}</script></span></span></li><li class="js-paper-rank-work_61803103 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="61803103"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 61803103, container: ".js-paper-rank-work_61803103", }); 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$(".js-view-count[data-work-id=61803103]").text(description); $(".js-view-count-work_61803103").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_61803103").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="61803103"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="2699" rel="nofollow" href="https://www.academia.edu/Documents/in/Tissue_Engineering">Tissue Engineering</a>, <script data-card-contents-for-ri="2699" type="text/json">{"id":2699,"name":"Tissue Engineering","url":"https://www.academia.edu/Documents/in/Tissue_Engineering?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7598" rel="nofollow" href="https://www.academia.edu/Documents/in/Rheology">Rheology</a>, <script data-card-contents-for-ri="7598" type="text/json">{"id":7598,"name":"Rheology","url":"https://www.academia.edu/Documents/in/Rheology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a><script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=61803103]'), work: {"id":61803103,"title":"Encapsulation of fibroblasts causes accelerated alginate hydrogel degradation","created_at":"2021-11-16T13:02:09.027-08:00","url":"https://www.academia.edu/61803103/Encapsulation_of_fibroblasts_causes_accelerated_alginate_hydrogel_degradation?f_ri=9534","dom_id":"work_61803103","summary":"Calcium-alginate hydrogel has been widely studied as a material for cell encapsulation for tissue engineering. At present, the effect that cells have on the degradation of alginate hydrogel is largely unknown. We have shown that fibroblasts encapsulated at a density of 7.5 Â 10 5 cells ml À1 in both 2% and 5% w/v alginate remain viable for at least 60 days. Rheological analysis was used to study how the mechanical properties exhibited by alginate hydrogel changed during 28 days in vitro culture. Alginate degradation was shown to occur throughout the study but was greatest within the first 7 days of culture for all samples, which correlated with a sharp release of calcium ions from the construct. Fibroblasts were shown to increase the rate of degradation during the first 7 days when compared with acellular samples in both 2% and 5% w/v gels, but after 28 days both acellular and cell-encapsulating samples retained disc-shaped morphologies and gel-like spectra. The results demonstrate that although at an early stage cells influence the mechanical properties of encapsulating alginate, over a longer period of culture, the hydrogels retain sufficient mechanical integrity to exhibit gel-like properties. This allows sustained immobilization of the cells at the desired location in vivo where they can produce extracellular matrix and growth factors to expedite the healing process.","downloadable_attachments":[{"id":74747460,"asset_id":61803103,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50938277,"first_name":"Nicola","last_name":"Hunt","domain_name":"independent","page_name":"NicolaHunt1","display_name":"Nicola Hunt","profile_url":"https://independent.academia.edu/NicolaHunt1?f_ri=9534","photo":"https://0.academia-photos.com/50938277/62919797/51218268/s65_nicola.hunt.png"}],"research_interests":[{"id":2699,"name":"Tissue Engineering","url":"https://www.academia.edu/Documents/in/Tissue_Engineering?f_ri=9534","nofollow":true},{"id":7598,"name":"Rheology","url":"https://www.academia.edu/Documents/in/Rheology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=9534","nofollow":true},{"id":29149,"name":"Extracellular Matrix","url":"https://www.academia.edu/Documents/in/Extracellular_Matrix?f_ri=9534"},{"id":46942,"name":"Cell Encapsulation","url":"https://www.academia.edu/Documents/in/Cell_Encapsulation?f_ri=9534"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534"},{"id":102727,"name":"Hydrogel","url":"https://www.academia.edu/Documents/in/Hydrogel?f_ri=9534"},{"id":387484,"name":"Alginates","url":"https://www.academia.edu/Documents/in/Alginates?f_ri=9534"},{"id":413750,"name":"In vitro culture","url":"https://www.academia.edu/Documents/in/In_vitro_culture?f_ri=9534"},{"id":444844,"name":"Spectrum analysis","url":"https://www.academia.edu/Documents/in/Spectrum_analysis?f_ri=9534"},{"id":951344,"name":"Growth Factor","url":"https://www.academia.edu/Documents/in/Growth_Factor?f_ri=9534"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival?f_ri=9534"},{"id":1902100,"name":"Biomechanical Phenomena","url":"https://www.academia.edu/Documents/in/Biomechanical_Phenomena?f_ri=9534"},{"id":2533047,"name":"fibroblasts","url":"https://www.academia.edu/Documents/in/fibroblasts?f_ri=9534"},{"id":2724508,"name":"Mechanical Property","url":"https://www.academia.edu/Documents/in/Mechanical_Property?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_60243233" data-work_id="60243233" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/60243233/Expression_of_bitter_taste_receptors_of_the_T2R_family_in_the_gastrointestinal_tract_and_enteroendocrine_STC_1_cells">Expression of bitter taste receptors of the T2R family in the gastrointestinal tract and enteroendocrine STC-1 cells</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Although a role for the gastric and intestinal mucosa in molecular sensing has been known for decades, the initial molecular recognition events that sense the chemical composition of the luminal contents has remained elusive. Here we... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_60243233" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Although a role for the gastric and intestinal mucosa in molecular sensing has been known for decades, the initial molecular recognition events that sense the chemical composition of the luminal contents has remained elusive. Here we identified putative taste receptor gene transcripts in the gastrointestinal tract. Our results, using reverse transcriptase-PCR, demonstrate the presence of transcripts corresponding to multiple members of the T2R family of bitter taste receptors in the antral and fundic gastric mucosa as well as in the lining of the duodenum. In addition, cDNA clones of T2R receptors were detected in a rat gastric endocrine cell cDNA library, suggesting that these receptors are expressed, at least partly, in enteroendocrine cells. Accordingly, expression of multiple T2R receptors also was found in STC-1 cells, an enteroendocrine cell line. The expression of ␣ subunits of G proteins implicated in intracellular taste signal transduction, namely G␣gust, and G␣t-2, also was demonstrated in the gastrointestinal mucosa as well as in STC-1 cells, as revealed by reverse transcriptase-PCR and DNA sequencing, immunohistochemistry, and Western blotting. Furthermore, addition of compounds widely used in bitter taste signaling (e.g., denatonium, phenylthiocarbamide, 6-n-propil-2thiouracil, and cycloheximide) to STC-1 cells promoted a rapid increase in intracellular Ca 2؉ concentration. These results demonstrate the expression of bitter taste receptors of the T2R family in the mouse and rat gastrointestinal tract. stomach ͉ intestine ͉ gustducin ͉ transducin</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/60243233" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8d2b2d78cbb962a0bfc383a1a2f93b5a" rel="nofollow" data-download="{"attachment_id":73775224,"asset_id":60243233,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/73775224/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50820724" href="https://independent.academia.edu/VincentWu21">Vincent Wu</a><script data-card-contents-for-user="50820724" type="text/json">{"id":50820724,"first_name":"Vincent","last_name":"Wu","domain_name":"independent","page_name":"VincentWu21","display_name":"Vincent Wu","profile_url":"https://independent.academia.edu/VincentWu21?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_60243233 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="60243233"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 60243233, container: ".js-paper-rank-work_60243233", }); 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$(".js-view-count[data-work-id=60243233]").text(description); $(".js-view-count-work_60243233").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_60243233").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="60243233"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="867" rel="nofollow" href="https://www.academia.edu/Documents/in/Perception">Perception</a>, <script data-card-contents-for-ri="867" type="text/json">{"id":867,"name":"Perception","url":"https://www.academia.edu/Documents/in/Perception?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a><script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=60243233]'), work: {"id":60243233,"title":"Expression of bitter taste receptors of the T2R family in the gastrointestinal tract and enteroendocrine STC-1 cells","created_at":"2021-10-28T17:01:34.741-07:00","url":"https://www.academia.edu/60243233/Expression_of_bitter_taste_receptors_of_the_T2R_family_in_the_gastrointestinal_tract_and_enteroendocrine_STC_1_cells?f_ri=9534","dom_id":"work_60243233","summary":"Although a role for the gastric and intestinal mucosa in molecular sensing has been known for decades, the initial molecular recognition events that sense the chemical composition of the luminal contents has remained elusive. Here we identified putative taste receptor gene transcripts in the gastrointestinal tract. Our results, using reverse transcriptase-PCR, demonstrate the presence of transcripts corresponding to multiple members of the T2R family of bitter taste receptors in the antral and fundic gastric mucosa as well as in the lining of the duodenum. In addition, cDNA clones of T2R receptors were detected in a rat gastric endocrine cell cDNA library, suggesting that these receptors are expressed, at least partly, in enteroendocrine cells. Accordingly, expression of multiple T2R receptors also was found in STC-1 cells, an enteroendocrine cell line. The expression of ␣ subunits of G proteins implicated in intracellular taste signal transduction, namely G␣gust, and G␣t-2, also was demonstrated in the gastrointestinal mucosa as well as in STC-1 cells, as revealed by reverse transcriptase-PCR and DNA sequencing, immunohistochemistry, and Western blotting. Furthermore, addition of compounds widely used in bitter taste signaling (e.g., denatonium, phenylthiocarbamide, 6-n-propil-2thiouracil, and cycloheximide) to STC-1 cells promoted a rapid increase in intracellular Ca 2؉ concentration. These results demonstrate the expression of bitter taste receptors of the T2R family in the mouse and rat gastrointestinal tract. stomach ͉ intestine ͉ gustducin ͉ transducin","downloadable_attachments":[{"id":73775224,"asset_id":60243233,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50820724,"first_name":"Vincent","last_name":"Wu","domain_name":"independent","page_name":"VincentWu21","display_name":"Vincent Wu","profile_url":"https://independent.academia.edu/VincentWu21?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":867,"name":"Perception","url":"https://www.academia.edu/Documents/in/Perception?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=9534","nofollow":true},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=9534","nofollow":true},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=9534"},{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction?f_ri=9534"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=9534"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534"},{"id":165437,"name":"Taste","url":"https://www.academia.edu/Documents/in/Taste?f_ri=9534"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning?f_ri=9534"},{"id":336223,"name":"Digestive System","url":"https://www.academia.edu/Documents/in/Digestive_System?f_ri=9534"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=9534"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=9534"},{"id":537759,"name":"Gastrointestinal Tract","url":"https://www.academia.edu/Documents/in/Gastrointestinal_Tract?f_ri=9534"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=9534"},{"id":2547982,"name":"Gastric mucosa","url":"https://www.academia.edu/Documents/in/Gastric_mucosa?f_ri=9534"},{"id":2950651,"name":"Tissue distribution","url":"https://www.academia.edu/Documents/in/Tissue_distribution?f_ri=9534"},{"id":3057016,"name":"Endocrine System","url":"https://www.academia.edu/Documents/in/Endocrine_System?f_ri=9534"},{"id":3067128,"name":"reverse transcriptase polymerase chain reaction","url":"https://www.academia.edu/Documents/in/reverse_transcriptase_polymerase_chain_reaction?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_49946739" data-work_id="49946739" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/49946739/Electrotonic_profile_and_passive_propagation_of_synaptic_potentials_in_three_subpopulations_of_hippocampal_CA1_interneurons">Electrotonic profile and passive propagation of synaptic potentials in three subpopulations of hippocampal CA1 interneurons</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">öTo elucidate the role of dendritic morphology in signal transfer, the passive propagation of somatic and dendritic potentials was compared in multi-compartment models of three interneuron subpopulations in the CA1 region. Nine... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_49946739" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">öTo elucidate the role of dendritic morphology in signal transfer, the passive propagation of somatic and dendritic potentials was compared in multi-compartment models of three interneuron subpopulations in the CA1 region. Nine calbindin-, 15 calretinin-and 10 parvalbumin-containing cells were modelled incorporating the detailed geometry, the currents of the action potentials in the soma, and the AMPA, N-methyl-D-aspartate and GABA-B receptor-mediated postsynaptic currents in the dendrites. The cable properties show characteristic di¡erences among the subpopulations. The morphotonic length of calbindin and calretinin cell dendrites is larger than of parvalbumin cells. Thus parvalbumin cells are more compact than calbindin or calretinin cells unless the ratio of their axial and membrane resistivities exceeds the ratios of the other two cell types by more than 33%. In calbindin cells, the distal parts of the extremely long dendrites that invade the alveus are virtually isolated from the soma for passively propagating signals. The synaptic potentials evoked at a given morphotonic distance from the soma show larger di¡erences locally on the dendrites than on the soma in all subpopulations. Both the somatic and dendritic amplitude ratios are the smallest in PV cells. In calbindin cells the somatic amplitude of synaptic potentials evoked at the same morphotonic distance from the soma is similar regardless of the number of branchpoints along their path. In calretinin and parvalbumin cells, from dendrites with long primary segments synaptic potentials reach the soma with larger amplitude than from dendrites that are branching close to the soma. The dendrites with the larger impact on somatic membrane potential are usually the dendrites that enter the stratum lacunosum-moleculare. These results indicate that dendritic morphology plays a role in changing the e¡ectiveness of synaptic potentials evoked at di¡erent dendritic locations, and in this way is likely to be an important factor in determining the integrative properties of the di¡erent neuron populations.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/49946739" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b67580f9f5c4db7215678dc2cd2cdd8e" rel="nofollow" data-download="{"attachment_id":68114572,"asset_id":49946739,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/68114572/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="36021244" href="https://independent.academia.edu/ZsuzsaEmri">Zsuzsa Emri</a><script data-card-contents-for-user="36021244" type="text/json">{"id":36021244,"first_name":"Zsuzsa","last_name":"Emri","domain_name":"independent","page_name":"ZsuzsaEmri","display_name":"Zsuzsa Emri","profile_url":"https://independent.academia.edu/ZsuzsaEmri?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_49946739 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="49946739"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 49946739, container: ".js-paper-rank-work_49946739", }); 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$(".js-view-count[data-work-id=49946739]").text(description); $(".js-view-count-work_49946739").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_49946739").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="49946739"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="161" rel="nofollow" href="https://www.academia.edu/Documents/in/Neuroscience">Neuroscience</a>, <script data-card-contents-for-ri="161" type="text/json">{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="221" rel="nofollow" href="https://www.academia.edu/Documents/in/Psychology">Psychology</a>, <script data-card-contents-for-ri="221" type="text/json">{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2007" rel="nofollow" href="https://www.academia.edu/Documents/in/Electrophysiology">Electrophysiology</a>, <script data-card-contents-for-ri="2007" type="text/json">{"id":2007,"name":"Electrophysiology","url":"https://www.academia.edu/Documents/in/Electrophysiology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a><script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=49946739]'), work: {"id":49946739,"title":"Electrotonic profile and passive propagation of synaptic potentials in three subpopulations of hippocampal CA1 interneurons","created_at":"2021-07-15T07:51:13.557-07:00","url":"https://www.academia.edu/49946739/Electrotonic_profile_and_passive_propagation_of_synaptic_potentials_in_three_subpopulations_of_hippocampal_CA1_interneurons?f_ri=9534","dom_id":"work_49946739","summary":"öTo elucidate the role of dendritic morphology in signal transfer, the passive propagation of somatic and dendritic potentials was compared in multi-compartment models of three interneuron subpopulations in the CA1 region. Nine calbindin-, 15 calretinin-and 10 parvalbumin-containing cells were modelled incorporating the detailed geometry, the currents of the action potentials in the soma, and the AMPA, N-methyl-D-aspartate and GABA-B receptor-mediated postsynaptic currents in the dendrites. The cable properties show characteristic di¡erences among the subpopulations. The morphotonic length of calbindin and calretinin cell dendrites is larger than of parvalbumin cells. Thus parvalbumin cells are more compact than calbindin or calretinin cells unless the ratio of their axial and membrane resistivities exceeds the ratios of the other two cell types by more than 33%. In calbindin cells, the distal parts of the extremely long dendrites that invade the alveus are virtually isolated from the soma for passively propagating signals. The synaptic potentials evoked at a given morphotonic distance from the soma show larger di¡erences locally on the dendrites than on the soma in all subpopulations. Both the somatic and dendritic amplitude ratios are the smallest in PV cells. In calbindin cells the somatic amplitude of synaptic potentials evoked at the same morphotonic distance from the soma is similar regardless of the number of branchpoints along their path. In calretinin and parvalbumin cells, from dendrites with long primary segments synaptic potentials reach the soma with larger amplitude than from dendrites that are branching close to the soma. The dendrites with the larger impact on somatic membrane potential are usually the dendrites that enter the stratum lacunosum-moleculare. These results indicate that dendritic morphology plays a role in changing the e¡ectiveness of synaptic potentials evoked at di¡erent dendritic locations, and in this way is likely to be an important factor in determining the integrative properties of the di¡erent neuron populations.","downloadable_attachments":[{"id":68114572,"asset_id":49946739,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36021244,"first_name":"Zsuzsa","last_name":"Emri","domain_name":"independent","page_name":"ZsuzsaEmri","display_name":"Zsuzsa Emri","profile_url":"https://independent.academia.edu/ZsuzsaEmri?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":161,"name":"Neuroscience","url":"https://www.academia.edu/Documents/in/Neuroscience?f_ri=9534","nofollow":true},{"id":221,"name":"Psychology","url":"https://www.academia.edu/Documents/in/Psychology?f_ri=9534","nofollow":true},{"id":2007,"name":"Electrophysiology","url":"https://www.academia.edu/Documents/in/Electrophysiology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":10866,"name":"Morphology","url":"https://www.academia.edu/Documents/in/Morphology?f_ri=9534"},{"id":57556,"name":"Hippocampus","url":"https://www.academia.edu/Documents/in/Hippocampus?f_ri=9534"},{"id":89802,"name":"Glutamate receptors","url":"https://www.academia.edu/Documents/in/Glutamate_receptors?f_ri=9534"},{"id":167027,"name":"Dendrites","url":"https://www.academia.edu/Documents/in/Dendrites?f_ri=9534"},{"id":291387,"name":"Mathematical Model","url":"https://www.academia.edu/Documents/in/Mathematical_Model?f_ri=9534"},{"id":535713,"name":"Dendrite","url":"https://www.academia.edu/Documents/in/Dendrite?f_ri=9534"},{"id":666669,"name":"Action potential","url":"https://www.academia.edu/Documents/in/Action_potential?f_ri=9534"},{"id":887736,"name":"Membrane Potential","url":"https://www.academia.edu/Documents/in/Membrane_Potential?f_ri=9534"},{"id":889628,"name":"Calcium Binding Proteins","url":"https://www.academia.edu/Documents/in/Calcium_Binding_Proteins?f_ri=9534"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=9534"},{"id":1287048,"name":"Interneurons","url":"https://www.academia.edu/Documents/in/Interneurons?f_ri=9534"},{"id":1877951,"name":"Cell Size","url":"https://www.academia.edu/Documents/in/Cell_Size?f_ri=9534"},{"id":2468093,"name":"Cell Membrane","url":"https://www.academia.edu/Documents/in/Cell_Membrane?f_ri=9534"},{"id":3476481,"name":"Compartment Model","url":"https://www.academia.edu/Documents/in/Compartment_Model?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_36185839" data-work_id="36185839" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/36185839/TRPC_channels_determine_human_keratinocyte_differentiation_New_insight_into_basal_cell_carcinoma">TRPC channels determine human keratinocyte differentiation: New insight into basal cell carcinoma</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Aberrant keratinocyte differentiation is considered to be a key mechanism in the onset of hyperproliferative dermatological diseases, including basal cell carcinoma (BCC). It is, therefore, vital to understand what drives keratinocytes to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_36185839" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Aberrant keratinocyte differentiation is considered to be a key mechanism in the onset of hyperproliferative dermatological diseases, including basal cell carcinoma (BCC). It is, therefore, vital to understand what drives keratinocytes to develop such pathological phenotypes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/36185839" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="20d1143500f993b926e4c01247134843" rel="nofollow" data-download="{"attachment_id":56086081,"asset_id":36185839,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/56086081/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="45164015" href="https://univ-lille.academia.edu/VyacheslavLehenkyi">V'yacheslav Lehen'kyi</a><script data-card-contents-for-user="45164015" type="text/json">{"id":45164015,"first_name":"V'yacheslav","last_name":"Lehen'kyi","domain_name":"univ-lille","page_name":"VyacheslavLehenkyi","display_name":"V'yacheslav Lehen'kyi","profile_url":"https://univ-lille.academia.edu/VyacheslavLehenkyi?f_ri=9534","photo":"https://0.academia-photos.com/45164015/18822192/18782158/s65_v_yacheslav.lehen_kyi.jpg"}</script></span></span></li><li class="js-paper-rank-work_36185839 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="36185839"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 36185839, container: ".js-paper-rank-work_36185839", }); 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It is, therefore, vital to understand what drives keratinocytes to develop such pathological phenotypes.","downloadable_attachments":[{"id":56086081,"asset_id":36185839,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":45164015,"first_name":"V'yacheslav","last_name":"Lehen'kyi","domain_name":"univ-lille","page_name":"VyacheslavLehenkyi","display_name":"V'yacheslav Lehen'kyi","profile_url":"https://univ-lille.academia.edu/VyacheslavLehenkyi?f_ri=9534","photo":"https://0.academia-photos.com/45164015/18822192/18782158/s65_v_yacheslav.lehen_kyi.jpg"}],"research_interests":[{"id":167,"name":"Physiology","url":"https://www.academia.edu/Documents/in/Physiology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology?f_ri=9534","nofollow":true},{"id":37793,"name":"Patch-clamp and imaging techniques","url":"https://www.academia.edu/Documents/in/Patch-clamp_and_imaging_techniques?f_ri=9534","nofollow":true},{"id":38132,"name":"Soc","url":"https://www.academia.edu/Documents/in/Soc?f_ri=9534"},{"id":57808,"name":"Cell line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=9534"},{"id":60436,"name":"Cell Differentiation","url":"https://www.academia.edu/Documents/in/Cell_Differentiation?f_ri=9534"},{"id":70552,"name":"Keratinocytes","url":"https://www.academia.edu/Documents/in/Keratinocytes?f_ri=9534"},{"id":74243,"name":"Skin Cancer","url":"https://www.academia.edu/Documents/in/Skin_Cancer?f_ri=9534"},{"id":122187,"name":"Endoplasmic Reticulum","url":"https://www.academia.edu/Documents/in/Endoplasmic_Reticulum?f_ri=9534"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=9534"},{"id":194810,"name":"Differentiation","url":"https://www.academia.edu/Documents/in/Differentiation?f_ri=9534"},{"id":518963,"name":"Basal cell carcinoma","url":"https://www.academia.edu/Documents/in/Basal_cell_carcinoma?f_ri=9534"},{"id":782254,"name":"Primary Culture","url":"https://www.academia.edu/Documents/in/Primary_Culture?f_ri=9534"},{"id":1036544,"name":"Knock out","url":"https://www.academia.edu/Documents/in/Knock_out?f_ri=9534"},{"id":1202042,"name":"Electric Conductivity","url":"https://www.academia.edu/Documents/in/Electric_Conductivity?f_ri=9534"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=9534"},{"id":2039739,"name":"Down-Regulation","url":"https://www.academia.edu/Documents/in/Down-Regulation?f_ri=9534"},{"id":2177223,"name":"Skin 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$(".js-view-count[data-work-id=34105917]").text(description); $(".js-view-count-work_34105917").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_34105917").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="34105917"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1605" rel="nofollow" href="https://www.academia.edu/Documents/in/Soil">Soil</a>, <script data-card-contents-for-ri="1605" type="text/json">{"id":1605,"name":"Soil","url":"https://www.academia.edu/Documents/in/Soil?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6791" rel="nofollow" href="https://www.academia.edu/Documents/in/Aging">Aging</a>, <script data-card-contents-for-ri="6791" type="text/json">{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="17158" rel="nofollow" href="https://www.academia.edu/Documents/in/Japan">Japan</a><script data-card-contents-for-ri="17158" type="text/json">{"id":17158,"name":"Japan","url":"https://www.academia.edu/Documents/in/Japan?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=34105917]'), work: {"id":34105917,"title":"The Pathogenesis of Amyotrophic Lateral Sclerosis","created_at":"2017-08-02T02:09:59.491-07:00","url":"https://www.academia.edu/34105917/The_Pathogenesis_of_Amyotrophic_Lateral_Sclerosis?f_ri=9534","dom_id":"work_34105917","summary":null,"downloadable_attachments":[{"id":54037453,"asset_id":34105917,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":66920582,"first_name":"Ludo Van Den","last_name":"Bosch","domain_name":"independent","page_name":"LudoVanDenBosch","display_name":"Ludo Van Den Bosch","profile_url":"https://independent.academia.edu/LudoVanDenBosch?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1605,"name":"Soil","url":"https://www.academia.edu/Documents/in/Soil?f_ri=9534","nofollow":true},{"id":6791,"name":"Aging","url":"https://www.academia.edu/Documents/in/Aging?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":17158,"name":"Japan","url":"https://www.academia.edu/Documents/in/Japan?f_ri=9534","nofollow":true},{"id":18496,"name":"Mining","url":"https://www.academia.edu/Documents/in/Mining?f_ri=9534"},{"id":29057,"name":"Manganese","url":"https://www.academia.edu/Documents/in/Manganese?f_ri=9534"},{"id":42973,"name":"Amyotrophic Lateral Sclerosis","url":"https://www.academia.edu/Documents/in/Amyotrophic_Lateral_Sclerosis?f_ri=9534"},{"id":64055,"name":"Water Supply","url":"https://www.academia.edu/Documents/in/Water_Supply?f_ri=9534"},{"id":147195,"name":"Central Nervous System","url":"https://www.academia.edu/Documents/in/Central_Nervous_System?f_ri=9534"},{"id":169532,"name":"Micronesia","url":"https://www.academia.edu/Documents/in/Micronesia?f_ri=9534"},{"id":243629,"name":"Lancet","url":"https://www.academia.edu/Documents/in/Lancet?f_ri=9534"},{"id":386342,"name":"Sodium","url":"https://www.academia.edu/Documents/in/Sodium?f_ri=9534"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=9534"},{"id":962276,"name":"Occupational Diseases","url":"https://www.academia.edu/Documents/in/Occupational_Diseases?f_ri=9534"},{"id":1312021,"name":"Environmental Exposure","url":"https://www.academia.edu/Documents/in/Environmental_Exposure?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_1554922" data-work_id="1554922" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/1554922/Ca2_ATPase_activity_and_Ca2_uptake_by_sarcoplasmic_reticulum_in_fish_heart_effects_of_thermal_acclimation">Ca2+-ATPase activity and Ca2+ uptake by sarcoplasmic reticulum in fish heart: effects of thermal acclimation</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Sarcoplasmic reticulum (SR) participates in the contraction and relaxation of cardiac muscle by a Ca 2+induced Ca 2+ release mechanism and an ATP-dependent resequestration process, respectively. Ryanodine-sensitive Ca 2+ efflux channels... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_1554922" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Sarcoplasmic reticulum (SR) participates in the contraction and relaxation of cardiac muscle by a Ca 2+induced Ca 2+ release mechanism and an ATP-dependent resequestration process, respectively. Ryanodine-sensitive Ca 2+ efflux channels are confined to the junctional and corbular SR, while thapsigargin-and cyclopiazonic-acidinhibitable Ca 2+ -pumping ATPase molecules are localized both in junctional and in nonjunctional SR . The significance of SR in the contraction-relaxation cycle of cardiac muscle varies greatly among different vertebrate classes, among different species within the same phylogenetic group and during the ontogenetic development of an individual. The SR is well developed in mammalian and avian cardiac myocytes, where it is thought to be the major source and sink of the activator Ca 2+ . In ectothermic vertebrates, such as lizards, frogs and fishes, and in newborn mammals, cardiac SR is relatively sparse, and contraction and relaxation are more directly dependent on sarcolemmal Ca 2+ fluxes Vornanen, 1996a,b).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/1554922" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="37211d97ec2848845ff86da5da0f8529" rel="nofollow" data-download="{"attachment_id":13455179,"asset_id":1554922,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/13455179/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="844893" href="https://uef.academia.edu/MattiVornanen">Matti Vornanen</a><script data-card-contents-for-user="844893" type="text/json">{"id":844893,"first_name":"Matti","last_name":"Vornanen","domain_name":"uef","page_name":"MattiVornanen","display_name":"Matti Vornanen","profile_url":"https://uef.academia.edu/MattiVornanen?f_ri=9534","photo":"https://0.academia-photos.com/844893/298514/3052868/s65_matti.vornanen.jpg"}</script></span></span></li><li class="js-paper-rank-work_1554922 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="1554922"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 1554922, container: ".js-paper-rank-work_1554922", }); });</script></li><li class="js-percentile-work_1554922 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 1554922; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_1554922"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_1554922 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="1554922"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 1554922; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=1554922]").text(description); $(".js-view-count-work_1554922").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_1554922").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="1554922"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12981" rel="nofollow" href="https://www.academia.edu/Documents/in/Enzyme_Inhibitors">Enzyme Inhibitors</a>, <script data-card-contents-for-ri="12981" type="text/json">{"id":12981,"name":"Enzyme Inhibitors","url":"https://www.academia.edu/Documents/in/Enzyme_Inhibitors?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="117270" rel="nofollow" href="https://www.academia.edu/Documents/in/Fishes">Fishes</a><script data-card-contents-for-ri="117270" type="text/json">{"id":117270,"name":"Fishes","url":"https://www.academia.edu/Documents/in/Fishes?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=1554922]'), work: {"id":1554922,"title":"Ca2+-ATPase activity and Ca2+ uptake by sarcoplasmic reticulum in fish heart: effects of thermal acclimation","created_at":"2012-05-12T22:57:15.099-07:00","url":"https://www.academia.edu/1554922/Ca2_ATPase_activity_and_Ca2_uptake_by_sarcoplasmic_reticulum_in_fish_heart_effects_of_thermal_acclimation?f_ri=9534","dom_id":"work_1554922","summary":"Sarcoplasmic reticulum (SR) participates in the contraction and relaxation of cardiac muscle by a Ca 2+induced Ca 2+ release mechanism and an ATP-dependent resequestration process, respectively. Ryanodine-sensitive Ca 2+ efflux channels are confined to the junctional and corbular SR, while thapsigargin-and cyclopiazonic-acidinhibitable Ca 2+ -pumping ATPase molecules are localized both in junctional and in nonjunctional SR . The significance of SR in the contraction-relaxation cycle of cardiac muscle varies greatly among different vertebrate classes, among different species within the same phylogenetic group and during the ontogenetic development of an individual. The SR is well developed in mammalian and avian cardiac myocytes, where it is thought to be the major source and sink of the activator Ca 2+ . In ectothermic vertebrates, such as lizards, frogs and fishes, and in newborn mammals, cardiac SR is relatively sparse, and contraction and relaxation are more directly dependent on sarcolemmal Ca 2+ fluxes Vornanen, 1996a,b).","downloadable_attachments":[{"id":13455179,"asset_id":1554922,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":844893,"first_name":"Matti","last_name":"Vornanen","domain_name":"uef","page_name":"MattiVornanen","display_name":"Matti Vornanen","profile_url":"https://uef.academia.edu/MattiVornanen?f_ri=9534","photo":"https://0.academia-photos.com/844893/298514/3052868/s65_matti.vornanen.jpg"}],"research_interests":[{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":12981,"name":"Enzyme Inhibitors","url":"https://www.academia.edu/Documents/in/Enzyme_Inhibitors?f_ri=9534","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534","nofollow":true},{"id":117270,"name":"Fishes","url":"https://www.academia.edu/Documents/in/Fishes?f_ri=9534","nofollow":true},{"id":240716,"name":"Body Temperature","url":"https://www.academia.edu/Documents/in/Body_Temperature?f_ri=9534"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=9534"},{"id":392828,"name":"Myocardium","url":"https://www.academia.edu/Documents/in/Myocardium?f_ri=9534"},{"id":708081,"name":"Carps","url":"https://www.academia.edu/Documents/in/Carps?f_ri=9534"},{"id":988721,"name":"Oncorhynchus Mykiss","url":"https://www.academia.edu/Documents/in/Oncorhynchus_Mykiss?f_ri=9534"},{"id":1764230,"name":"Experimental Biology","url":"https://www.academia.edu/Documents/in/Experimental_Biology?f_ri=9534"},{"id":2350842,"name":"Sarcoplasmic reticulum","url":"https://www.academia.edu/Documents/in/Sarcoplasmic_reticulum?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_2648685" data-work_id="2648685" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/2648685/Expression_and_function_of_the_neural_cell_adhesion_molecule_L1_in_mouse_leukocytes">Expression and function of the neural cell adhesion molecule L1 in mouse leukocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The neural cell adhesion molecule L1 is a cell surface glycoprotein of the immunoglobulin superfamily which mediates adhesion between neural cells. The possibility that similar cell-cell recognition mechanisms may be shared by the nervous... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_2648685" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The neural cell adhesion molecule L1 is a cell surface glycoprotein of the immunoglobulin superfamily which mediates adhesion between neural cells. The possibility that similar cell-cell recognition mechanisms may be shared by the nervous and immune systems prompted us to study the expression and function of L1 in cells of the hematopoietic system. Immunofluorescence analysis using monoclonal L1 antibody revealed that the molecule is expressed in the bone marrow, spleen, and thymus of the mouse. This observation was confirmed by amplifying cDNA derived from these organs by the polymerase chain reaction with L1-specific oligonucleotide primers. Two-color fluorescence analysis indicated that bone marrow lymphoid and granulocyte precursor cells express low and high levels of L1, respectively. In the thymus L1 is primarily expressed by mature cells that have a strong expression of CD3 and in the spleen both B cells and T cells express L1.The possible function of L1 in lymphoid cells was studied using subcloned ESb-MP lymphoma cells having high or low densities of L1 on the cell surface as well as activated splenic B lymphoblasts. Parental and subcloned ESb-MP cells that strongly expressed L1 could form homotypic aggregates in the presence of low Ca2+ levels, whereas subcloned ESb-MP cells with a weak expression of L1 did not aggregate, suggesting that L1 mediates the Ca2+-independent aggregation of the parental ESb-MP cells. Furthermore, the aggregation of activated B lymphoblasts under physiological concentrations of Ca2+ and Mg2+ was inhibited by 30% in the presence of Fab fragments of polyclonal L1 antibodies, implying that L1 also mediates adhesion among normal lymphoid cells. A possible role of L1 on lymphocytes in stimulating the innervation of lymphoid organs is discussed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/2648685" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f609175b37b97918981c180c26764d87" rel="nofollow" data-download="{"attachment_id":50553267,"asset_id":2648685,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50553267/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="3371732" href="https://uni-heidelberg.academia.edu/GuniKadmon">Guni Kadmon</a><script data-card-contents-for-user="3371732" type="text/json">{"id":3371732,"first_name":"Guni","last_name":"Kadmon","domain_name":"uni-heidelberg","page_name":"GuniKadmon","display_name":"Guni Kadmon","profile_url":"https://uni-heidelberg.academia.edu/GuniKadmon?f_ri=9534","photo":"https://0.academia-photos.com/3371732/2213546/2592119/s65_guni.kadmon.jpg"}</script></span></span></li><li class="js-paper-rank-work_2648685 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="2648685"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 2648685, container: ".js-paper-rank-work_2648685", }); });</script></li><li class="js-percentile-work_2648685 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 2648685; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_2648685"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_2648685 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="2648685"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 2648685; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=2648685]").text(description); $(".js-view-count-work_2648685").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_2648685").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="2648685"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="1290" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" rel="nofollow" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="159239" rel="nofollow" href="https://www.academia.edu/Documents/in/Bone_marrow">Bone marrow</a><script data-card-contents-for-ri="159239" type="text/json">{"id":159239,"name":"Bone marrow","url":"https://www.academia.edu/Documents/in/Bone_marrow?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=2648685]'), work: {"id":2648685,"title":"Expression and function of the neural cell adhesion molecule L1 in mouse leukocytes","created_at":"2013-02-25T15:04:03.385-08:00","url":"https://www.academia.edu/2648685/Expression_and_function_of_the_neural_cell_adhesion_molecule_L1_in_mouse_leukocytes?f_ri=9534","dom_id":"work_2648685","summary":"The neural cell adhesion molecule L1 is a cell surface glycoprotein of the immunoglobulin superfamily which mediates adhesion between neural cells. The possibility that similar cell-cell recognition mechanisms may be shared by the nervous and immune systems prompted us to study the expression and function of L1 in cells of the hematopoietic system. Immunofluorescence analysis using monoclonal L1 antibody revealed that the molecule is expressed in the bone marrow, spleen, and thymus of the mouse. This observation was confirmed by amplifying cDNA derived from these organs by the polymerase chain reaction with L1-specific oligonucleotide primers. Two-color fluorescence analysis indicated that bone marrow lymphoid and granulocyte precursor cells express low and high levels of L1, respectively. In the thymus L1 is primarily expressed by mature cells that have a strong expression of CD3 and in the spleen both B cells and T cells express L1.The possible function of L1 in lymphoid cells was studied using subcloned ESb-MP lymphoma cells having high or low densities of L1 on the cell surface as well as activated splenic B lymphoblasts. Parental and subcloned ESb-MP cells that strongly expressed L1 could form homotypic aggregates in the presence of low Ca2+ levels, whereas subcloned ESb-MP cells with a weak expression of L1 did not aggregate, suggesting that L1 mediates the Ca2+-independent aggregation of the parental ESb-MP cells. Furthermore, the aggregation of activated B lymphoblasts under physiological concentrations of Ca2+ and Mg2+ was inhibited by 30% in the presence of Fab fragments of polyclonal L1 antibodies, implying that L1 also mediates adhesion among normal lymphoid cells. A possible role of L1 on lymphocytes in stimulating the innervation of lymphoid organs is discussed.","downloadable_attachments":[{"id":50553267,"asset_id":2648685,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":3371732,"first_name":"Guni","last_name":"Kadmon","domain_name":"uni-heidelberg","page_name":"GuniKadmon","display_name":"Guni Kadmon","profile_url":"https://uni-heidelberg.academia.edu/GuniKadmon?f_ri=9534","photo":"https://0.academia-photos.com/3371732/2213546/2592119/s65_guni.kadmon.jpg"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534","nofollow":true},{"id":159239,"name":"Bone marrow","url":"https://www.academia.edu/Documents/in/Bone_marrow?f_ri=9534","nofollow":true},{"id":678853,"name":"B Lymphocytes","url":"https://www.academia.edu/Documents/in/B_Lymphocytes?f_ri=9534"},{"id":1272099,"name":"Leukocytes","url":"https://www.academia.edu/Documents/in/Leukocytes?f_ri=9534"},{"id":1425658,"name":"Lymphoid Tissue","url":"https://www.academia.edu/Documents/in/Lymphoid_Tissue?f_ri=9534"},{"id":2524494,"name":"Cell Aggregation","url":"https://www.academia.edu/Documents/in/Cell_Aggregation?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6914541" data-work_id="6914541" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6914541/Influence_of_calcium_hydroxide_on_the_fate_of_perfluorooctanesulfonate_under_thermal_conditions">Influence of calcium hydroxide on the fate of perfluorooctanesulfonate under thermal conditions</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To explore the potential fate and transport of perfluorochemicals in the thermal treatment of sludge, perfluorooctanesulfonate (PFOS), a perfluorochemical species commonly dominant in wastewater sludge, was mixed with hydrated lime... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6914541" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To explore the potential fate and transport of perfluorochemicals in the thermal treatment of sludge, perfluorooctanesulfonate (PFOS), a perfluorochemical species commonly dominant in wastewater sludge, was mixed with hydrated lime (Ca(OH) 2 ) to quantitatively observe their interaction under different temperatures. The phase compositions of the mixtures after the reactions were qualitatively identified and quantitatively determined using X-ray diffraction technique. The results of the thermogravimetry and differential scanning calorimetry analyses indicate that PFOS gasified directly during the thermal treatment process when the temperature was increased to around 425 • C. However, the formation of CaF 2 at 350 • C suggests that the presence of Ca(OH) 2 in the mixture can lead to the decomposition of PFOS at 350 • C, which is lower than the decomposition temperature of PFOS alone (425 • C). The increase of temperature promoted a solid state reaction between PFOS and Ca(OH) 2 , and also enhanced the interaction between the gaseous products of PFOS and CaO (or Ca(OH) 2 ). The preferred Ca/F molar ratio to achieve fluorine stabilization by Ca(OH) 2 was above 1:1 in the experiment involving 400 • C and 600 • C treatment. It also showed that equilibrium efficiency is achieved within 5 min at 400 • C and within 1 min above 600 • C.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6914541" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="93c9871cb403f7dbe3a998674747ab82" rel="nofollow" data-download="{"attachment_id":48671156,"asset_id":6914541,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48671156/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="11640190" href="https://independent.academia.edu/xingwenlu">xingwen lu</a><script data-card-contents-for-user="11640190" type="text/json">{"id":11640190,"first_name":"xingwen","last_name":"lu","domain_name":"independent","page_name":"xingwenlu","display_name":"xingwen lu","profile_url":"https://independent.academia.edu/xingwenlu?f_ri=9534","photo":"https://0.academia-photos.com/11640190/27483554/25827851/s65_xingwen.lu.jpg"}</script></span></span></li><li class="js-paper-rank-work_6914541 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6914541"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6914541, container: ".js-paper-rank-work_6914541", }); });</script></li><li class="js-percentile-work_6914541 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 6914541; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_6914541"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_6914541 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="6914541"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 6914541; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=6914541]").text(description); $(".js-view-count-work_6914541").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6914541").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6914541"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" rel="nofollow" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="6515" rel="nofollow" href="https://www.academia.edu/Documents/in/Water_Purification">Water Purification</a>, <script data-card-contents-for-ri="6515" type="text/json">{"id":6515,"name":"Water Purification","url":"https://www.academia.edu/Documents/in/Water_Purification?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="78753" rel="nofollow" href="https://www.academia.edu/Documents/in/Differential_scanning_calorimetry">Differential scanning calorimetry</a><script data-card-contents-for-ri="78753" type="text/json">{"id":78753,"name":"Differential scanning calorimetry","url":"https://www.academia.edu/Documents/in/Differential_scanning_calorimetry?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6914541]'), work: {"id":6914541,"title":"Influence of calcium hydroxide on the fate of perfluorooctanesulfonate under thermal conditions","created_at":"2014-04-29T19:31:38.037-07:00","url":"https://www.academia.edu/6914541/Influence_of_calcium_hydroxide_on_the_fate_of_perfluorooctanesulfonate_under_thermal_conditions?f_ri=9534","dom_id":"work_6914541","summary":"To explore the potential fate and transport of perfluorochemicals in the thermal treatment of sludge, perfluorooctanesulfonate (PFOS), a perfluorochemical species commonly dominant in wastewater sludge, was mixed with hydrated lime (Ca(OH) 2 ) to quantitatively observe their interaction under different temperatures. The phase compositions of the mixtures after the reactions were qualitatively identified and quantitatively determined using X-ray diffraction technique. The results of the thermogravimetry and differential scanning calorimetry analyses indicate that PFOS gasified directly during the thermal treatment process when the temperature was increased to around 425 • C. However, the formation of CaF 2 at 350 • C suggests that the presence of Ca(OH) 2 in the mixture can lead to the decomposition of PFOS at 350 • C, which is lower than the decomposition temperature of PFOS alone (425 • C). The increase of temperature promoted a solid state reaction between PFOS and Ca(OH) 2 , and also enhanced the interaction between the gaseous products of PFOS and CaO (or Ca(OH) 2 ). The preferred Ca/F molar ratio to achieve fluorine stabilization by Ca(OH) 2 was above 1:1 in the experiment involving 400 • C and 600 • C treatment. It also showed that equilibrium efficiency is achieved within 5 min at 400 • C and within 1 min above 600 • C.","downloadable_attachments":[{"id":48671156,"asset_id":6914541,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":11640190,"first_name":"xingwen","last_name":"lu","domain_name":"independent","page_name":"xingwenlu","display_name":"xingwen lu","profile_url":"https://independent.academia.edu/xingwenlu?f_ri=9534","photo":"https://0.academia-photos.com/11640190/27483554/25827851/s65_xingwen.lu.jpg"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=9534","nofollow":true},{"id":6515,"name":"Water Purification","url":"https://www.academia.edu/Documents/in/Water_Purification?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":78753,"name":"Differential scanning calorimetry","url":"https://www.academia.edu/Documents/in/Differential_scanning_calorimetry?f_ri=9534","nofollow":true},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=9534"},{"id":149625,"name":"Hazardous Materials","url":"https://www.academia.edu/Documents/in/Hazardous_Materials?f_ri=9534"},{"id":246949,"name":"Thermogravimetry","url":"https://www.academia.edu/Documents/in/Thermogravimetry?f_ri=9534"},{"id":315203,"name":"Fluorine","url":"https://www.academia.edu/Documents/in/Fluorine?f_ri=9534"},{"id":386527,"name":"X ray diffraction","url":"https://www.academia.edu/Documents/in/X_ray_diffraction?f_ri=9534"},{"id":394503,"name":"Thermal Treatment","url":"https://www.academia.edu/Documents/in/Thermal_Treatment?f_ri=9534"},{"id":410387,"name":"Sewage","url":"https://www.academia.edu/Documents/in/Sewage?f_ri=9534"},{"id":469004,"name":"Calcium Hydroxide","url":"https://www.academia.edu/Documents/in/Calcium_Hydroxide?f_ri=9534"},{"id":823821,"name":"Solid State Reaction","url":"https://www.academia.edu/Documents/in/Solid_State_Reaction?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27284395" data-work_id="27284395" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27284395/Immunohistochemistry_and_microwave_decalcification_of_human_temporal_bones">Immunohistochemistry and microwave decalcification of human temporal bones</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Processing of human temporal bones is a long, expensive process and the resulting celloidin sections are difficult to use for immunohistochemistry. We tested the ability of immunohistochemical assays to work in human temporal bones that... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_27284395" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Processing of human temporal bones is a long, expensive process and the resulting celloidin sections are difficult to use for immunohistochemistry. We tested the ability of immunohistochemical assays to work in human temporal bones that were decalcified using a microwave oven. Tissue was trimmed to an approximate cube (1.5^2 cm/side) containing only the cochlea and immersed in fresh EDTA with paraformaldehyde every 6 h. This sized block required 190^400 h to decalcify. The decalcified tissue was embedded in paraffin and sectioned. Sections were immunoassayed with anti-cytochrome c oxidase, anti-neurofilament or anti-peripherin. All three antibodies labeled the appropriate structures. This procedure may stimulate advancement in the understanding of human inner ear pathology. ß</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27284395" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="69c9851177b900176194d417289d9b8f" rel="nofollow" data-download="{"attachment_id":47543599,"asset_id":27284395,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47543599/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51389337" href="https://independent.academia.edu/BillingsPeter">Peter Billings</a><script data-card-contents-for-user="51389337" type="text/json">{"id":51389337,"first_name":"Peter","last_name":"Billings","domain_name":"independent","page_name":"BillingsPeter","display_name":"Peter Billings","profile_url":"https://independent.academia.edu/BillingsPeter?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_27284395 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="27284395"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 27284395, container: ".js-paper-rank-work_27284395", }); });</script></li><li class="js-percentile-work_27284395 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 27284395; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_27284395"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_27284395 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="27284395"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 27284395; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=27284395]").text(description); $(".js-view-count-work_27284395").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_27284395").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="27284395"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="5023" rel="nofollow" href="https://www.academia.edu/Documents/in/Microwave">Microwave</a>, <script data-card-contents-for-ri="5023" type="text/json">{"id":5023,"name":"Microwave","url":"https://www.academia.edu/Documents/in/Microwave?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="59693" rel="nofollow" href="https://www.academia.edu/Documents/in/Hearing">Hearing</a><script data-card-contents-for-ri="59693" type="text/json">{"id":59693,"name":"Hearing","url":"https://www.academia.edu/Documents/in/Hearing?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=27284395]'), work: {"id":27284395,"title":"Immunohistochemistry and microwave decalcification of human temporal bones","created_at":"2016-07-26T15:40:46.932-07:00","url":"https://www.academia.edu/27284395/Immunohistochemistry_and_microwave_decalcification_of_human_temporal_bones?f_ri=9534","dom_id":"work_27284395","summary":"Processing of human temporal bones is a long, expensive process and the resulting celloidin sections are difficult to use for immunohistochemistry. We tested the ability of immunohistochemical assays to work in human temporal bones that were decalcified using a microwave oven. Tissue was trimmed to an approximate cube (1.5^2 cm/side) containing only the cochlea and immersed in fresh EDTA with paraformaldehyde every 6 h. This sized block required 190^400 h to decalcify. The decalcified tissue was embedded in paraffin and sectioned. Sections were immunoassayed with anti-cytochrome c oxidase, anti-neurofilament or anti-peripherin. All three antibodies labeled the appropriate structures. This procedure may stimulate advancement in the understanding of human inner ear pathology. ß","downloadable_attachments":[{"id":47543599,"asset_id":27284395,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51389337,"first_name":"Peter","last_name":"Billings","domain_name":"independent","page_name":"BillingsPeter","display_name":"Peter Billings","profile_url":"https://independent.academia.edu/BillingsPeter?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5023,"name":"Microwave","url":"https://www.academia.edu/Documents/in/Microwave?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=9534","nofollow":true},{"id":59693,"name":"Hearing","url":"https://www.academia.edu/Documents/in/Hearing?f_ri=9534","nofollow":true},{"id":121939,"name":"Technique","url":"https://www.academia.edu/Documents/in/Technique?f_ri=9534"},{"id":132510,"name":"Cytochrome c oxidase","url":"https://www.academia.edu/Documents/in/Cytochrome_c_oxidase?f_ri=9534"},{"id":186234,"name":"Medical Physiology","url":"https://www.academia.edu/Documents/in/Medical_Physiology?f_ri=9534"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":439473,"name":"Microwaves","url":"https://www.academia.edu/Documents/in/Microwaves?f_ri=9534"},{"id":815168,"name":"Temporal Bone","url":"https://www.academia.edu/Documents/in/Temporal_Bone?f_ri=9534"},{"id":1151963,"name":"Cochlea","url":"https://www.academia.edu/Documents/in/Cochlea?f_ri=9534"},{"id":1239755,"name":"Neurosciences","url":"https://www.academia.edu/Documents/in/Neurosciences?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24477344" data-work_id="24477344" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24477344/A_Theoretical_Model_of_Slow_Wave_Regulation_Using_Voltage_Dependent_Synthesis_of_Inositol_1_4_5_Trisphosphate">A Theoretical Model of Slow Wave Regulation Using Voltage-Dependent Synthesis of Inositol 1,4,5-Trisphosphate</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A qualitative mathematical model is presented that examines membrane potential feedback on synthesis of inositol 1,4,5-trisphosphate (IP 3 ), and its role in generation and modulation of slow waves. Previous experimental studies indicate... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24477344" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A qualitative mathematical model is presented that examines membrane potential feedback on synthesis of inositol 1,4,5-trisphosphate (IP 3 ), and its role in generation and modulation of slow waves. Previous experimental studies indicate that slow waves show voltage dependence, and this is likely to result through membrane potential modulation of IP 3 . It is proposed that the observed response of the tissue to current pulse, pulse train, and maintained current injection can be explained by changes in IP 3 , modulated through a voltage-IP 3 feedback loop. Differences underlying the tissue responses to current injections of opposite polarities are shown to be due to the sequence of events following such currents. Results from this model are consistent with experimental findings and provide further understanding of these experimental observations. Specifically, we find that membrane potential can induce, abolish, and modulate slow wave frequency by altering the excitability of the tissue through the voltage-IP 3 feedback loop.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24477344" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="99c931f48908a270f9d93fe7fb538d2a" rel="nofollow" data-download="{"attachment_id":44811180,"asset_id":24477344,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44811180/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="47077852" href="https://independent.academia.edu/DirkHelden">Dirk Helden</a><script data-card-contents-for-user="47077852" type="text/json">{"id":47077852,"first_name":"Dirk","last_name":"Helden","domain_name":"independent","page_name":"DirkHelden","display_name":"Dirk Helden","profile_url":"https://independent.academia.edu/DirkHelden?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24477344 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24477344"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24477344, container: ".js-paper-rank-work_24477344", }); });</script></li><li class="js-percentile-work_24477344 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24477344; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_24477344"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_24477344 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="24477344"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24477344; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24477344]").text(description); $(".js-view-count-work_24477344").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24477344").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24477344"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="502" rel="nofollow" href="https://www.academia.edu/Documents/in/Biophysics">Biophysics</a>, <script data-card-contents-for-ri="502" type="text/json">{"id":502,"name":"Biophysics","url":"https://www.academia.edu/Documents/in/Biophysics?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="2007" rel="nofollow" href="https://www.academia.edu/Documents/in/Electrophysiology">Electrophysiology</a>, <script data-card-contents-for-ri="2007" type="text/json">{"id":2007,"name":"Electrophysiology","url":"https://www.academia.edu/Documents/in/Electrophysiology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" rel="nofollow" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a><script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24477344]'), work: {"id":24477344,"title":"A Theoretical Model of Slow Wave Regulation Using Voltage-Dependent Synthesis of Inositol 1,4,5-Trisphosphate","created_at":"2016-04-16T23:26:15.583-07:00","url":"https://www.academia.edu/24477344/A_Theoretical_Model_of_Slow_Wave_Regulation_Using_Voltage_Dependent_Synthesis_of_Inositol_1_4_5_Trisphosphate?f_ri=9534","dom_id":"work_24477344","summary":"A qualitative mathematical model is presented that examines membrane potential feedback on synthesis of inositol 1,4,5-trisphosphate (IP 3 ), and its role in generation and modulation of slow waves. Previous experimental studies indicate that slow waves show voltage dependence, and this is likely to result through membrane potential modulation of IP 3 . It is proposed that the observed response of the tissue to current pulse, pulse train, and maintained current injection can be explained by changes in IP 3 , modulated through a voltage-IP 3 feedback loop. Differences underlying the tissue responses to current injections of opposite polarities are shown to be due to the sequence of events following such currents. Results from this model are consistent with experimental findings and provide further understanding of these experimental observations. Specifically, we find that membrane potential can induce, abolish, and modulate slow wave frequency by altering the excitability of the tissue through the voltage-IP 3 feedback loop.","downloadable_attachments":[{"id":44811180,"asset_id":24477344,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":47077852,"first_name":"Dirk","last_name":"Helden","domain_name":"independent","page_name":"DirkHelden","display_name":"Dirk Helden","profile_url":"https://independent.academia.edu/DirkHelden?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":502,"name":"Biophysics","url":"https://www.academia.edu/Documents/in/Biophysics?f_ri=9534","nofollow":true},{"id":2007,"name":"Electrophysiology","url":"https://www.academia.edu/Documents/in/Electrophysiology?f_ri=9534","nofollow":true},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":19156,"name":"Biophysical Chemistry","url":"https://www.academia.edu/Documents/in/Biophysical_Chemistry?f_ri=9534"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534"},{"id":65171,"name":"Smooth muscle","url":"https://www.academia.edu/Documents/in/Smooth_muscle?f_ri=9534"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences?f_ri=9534"},{"id":215075,"name":"Experimental Study","url":"https://www.academia.edu/Documents/in/Experimental_Study?f_ri=9534"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=9534"},{"id":291387,"name":"Mathematical Model","url":"https://www.academia.edu/Documents/in/Mathematical_Model?f_ri=9534"},{"id":343667,"name":"Theoretical Models","url":"https://www.academia.edu/Documents/in/Theoretical_Models?f_ri=9534"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=9534"},{"id":614190,"name":"Feedback loop","url":"https://www.academia.edu/Documents/in/Feedback_loop?f_ri=9534"},{"id":887736,"name":"Membrane Potential","url":"https://www.academia.edu/Documents/in/Membrane_Potential?f_ri=9534"},{"id":1154248,"name":"Theoretical Model","url":"https://www.academia.edu/Documents/in/Theoretical_Model?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22082257" data-work_id="22082257" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22082257/First_Principles_Simulations_of_the_Structural_and_Dynamical_Properties_of_Hydrated_Metal_Ions_Me2_and_Solvated_Metal_Carbonates_Me_Ca_Mg_and_Sr_">First Principles Simulations of the Structural and Dynamical Properties of Hydrated Metal Ions Me2+ and Solvated Metal Carbonates (Me = Ca, Mg, and Sr)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The structural and dynamical properties of the alkaline earth metal ions Mg 2þ , Ca 2þ , and Sr 2þ and their carbonate and bicarbonate complexes in aqueous solution are examined through first principles molecular dynamics simulations... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_22082257" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The structural and dynamical properties of the alkaline earth metal ions Mg 2þ , Ca 2þ , and Sr 2þ and their carbonate and bicarbonate complexes in aqueous solution are examined through first principles molecular dynamics simulations based on the density functional theory. Calculations were conducted in explicit heavy water molecules and at the average temperature of 400 K, conditions which are necessary to obtain a liquid-like water structure and diffusion time-scales when using gradient corrected density functionals. According to these simulations, the magnesium ion undergoes a significant contraction of its coordination sphere in the Mg(H)CO 3 (þ) aqueous complex, whereas calcium and strontium increase their average first shell coordination number when coordinated to HCO 3 or CO 3 2-</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22082257" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0c477d087535cacf412647ab5f2f56b0" rel="nofollow" data-download="{"attachment_id":42756724,"asset_id":22082257,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42756724/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="43380406" href="https://independent.academia.edu/NoraDeLeeuw">Nora De Leeuw</a><script data-card-contents-for-user="43380406" type="text/json">{"id":43380406,"first_name":"Nora De","last_name":"Leeuw","domain_name":"independent","page_name":"NoraDeLeeuw","display_name":"Nora De Leeuw","profile_url":"https://independent.academia.edu/NoraDeLeeuw?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_22082257 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="22082257"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 22082257, container: ".js-paper-rank-work_22082257", }); 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$(".js-view-count[data-work-id=22082257]").text(description); $(".js-view-count-work_22082257").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_22082257").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="22082257"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="56" rel="nofollow" href="https://www.academia.edu/Documents/in/Materials_Engineering">Materials Engineering</a>, <script data-card-contents-for-ri="56" type="text/json">{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="530" rel="nofollow" href="https://www.academia.edu/Documents/in/Inorganic_Chemistry">Inorganic Chemistry</a>, <script data-card-contents-for-ri="530" type="text/json">{"id":530,"name":"Inorganic Chemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Chemistry?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="35829" rel="nofollow" href="https://www.academia.edu/Documents/in/Aqueous_Solutions">Aqueous Solutions</a><script data-card-contents-for-ri="35829" type="text/json">{"id":35829,"name":"Aqueous Solutions","url":"https://www.academia.edu/Documents/in/Aqueous_Solutions?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22082257]'), work: {"id":22082257,"title":"First Principles Simulations of the Structural and Dynamical Properties of Hydrated Metal Ions Me2+ and Solvated Metal Carbonates (Me = Ca, Mg, and Sr)","created_at":"2016-02-17T00:38:07.611-08:00","url":"https://www.academia.edu/22082257/First_Principles_Simulations_of_the_Structural_and_Dynamical_Properties_of_Hydrated_Metal_Ions_Me2_and_Solvated_Metal_Carbonates_Me_Ca_Mg_and_Sr_?f_ri=9534","dom_id":"work_22082257","summary":"The structural and dynamical properties of the alkaline earth metal ions Mg 2þ , Ca 2þ , and Sr 2þ and their carbonate and bicarbonate complexes in aqueous solution are examined through first principles molecular dynamics simulations based on the density functional theory. Calculations were conducted in explicit heavy water molecules and at the average temperature of 400 K, conditions which are necessary to obtain a liquid-like water structure and diffusion time-scales when using gradient corrected density functionals. According to these simulations, the magnesium ion undergoes a significant contraction of its coordination sphere in the Mg(H)CO 3 (þ) aqueous complex, whereas calcium and strontium increase their average first shell coordination number when coordinated to HCO 3 or CO 3 2-","downloadable_attachments":[{"id":42756724,"asset_id":22082257,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":43380406,"first_name":"Nora De","last_name":"Leeuw","domain_name":"independent","page_name":"NoraDeLeeuw","display_name":"Nora De Leeuw","profile_url":"https://independent.academia.edu/NoraDeLeeuw?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=9534","nofollow":true},{"id":530,"name":"Inorganic Chemistry","url":"https://www.academia.edu/Documents/in/Inorganic_Chemistry?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":35829,"name":"Aqueous Solutions","url":"https://www.academia.edu/Documents/in/Aqueous_Solutions?f_ri=9534","nofollow":true},{"id":83315,"name":"Diffusion","url":"https://www.academia.edu/Documents/in/Diffusion?f_ri=9534"},{"id":590943,"name":"Metal ion","url":"https://www.academia.edu/Documents/in/Metal_ion?f_ri=9534"},{"id":839387,"name":"Monomers","url":"https://www.academia.edu/Documents/in/Monomers?f_ri=9534"},{"id":1874422,"name":"Dynamic Properties","url":"https://www.academia.edu/Documents/in/Dynamic_Properties?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21022668" data-work_id="21022668" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21022668/Vitamin_D_and_musculoskeletal_health_cardiovascular_disease_autoimmunity_and_cancer_Recommendations_for_clinical_practice">Vitamin D and musculoskeletal health, cardiovascular disease, autoimmunity and cancer: Recommendations for clinical practice</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest">Background: There is increasing evidence that, in addition to the well-known effects on musculoskeletal health, vitamin D status may be related to a number of non-skeletal diseases.</div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21022668" 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window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21022668]").text(description); $(".js-view-count-work_21022668").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21022668").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21022668"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" rel="nofollow" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4531" rel="nofollow" href="https://www.academia.edu/Documents/in/Clinical_Trial">Clinical Trial</a>, <script data-card-contents-for-ri="4531" type="text/json">{"id":4531,"name":"Clinical Trial","url":"https://www.academia.edu/Documents/in/Clinical_Trial?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9531" rel="nofollow" href="https://www.academia.edu/Documents/in/Vitamin_D">Vitamin D</a>, <script data-card-contents-for-ri="9531" type="text/json">{"id":9531,"name":"Vitamin D","url":"https://www.academia.edu/Documents/in/Vitamin_D?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a><script data-card-contents-for-ri="9534" 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diseases.","downloadable_attachments":[{"id":41675437,"asset_id":21022668,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42111359,"first_name":"sara","last_name":"gandini","domain_name":"independent","page_name":"saragandini1","display_name":"sara gandini","profile_url":"https://independent.academia.edu/saragandini1?f_ri=9534","photo":"https://0.academia-photos.com/42111359/11503214/12830053/s65_sara.gandini.jpg"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=9534","nofollow":true},{"id":4531,"name":"Clinical Trial","url":"https://www.academia.edu/Documents/in/Clinical_Trial?f_ri=9534","nofollow":true},{"id":9531,"name":"Vitamin D","url":"https://www.academia.edu/Documents/in/Vitamin_D?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":12320,"name":"Autoimmunity","url":"https://www.academia.edu/Documents/in/Autoimmunity?f_ri=9534"},{"id":24735,"name":"Cardiovascular disease","url":"https://www.academia.edu/Documents/in/Cardiovascular_disease?f_ri=9534"},{"id":52836,"name":"Clinical Practice","url":"https://www.academia.edu/Documents/in/Clinical_Practice?f_ri=9534"},{"id":133057,"name":"Young Adult","url":"https://www.academia.edu/Documents/in/Young_Adult?f_ri=9534"},{"id":147861,"name":"Expert Opinion","url":"https://www.academia.edu/Documents/in/Expert_Opinion?f_ri=9534"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=9534"},{"id":324154,"name":"Immune system","url":"https://www.academia.edu/Documents/in/Immune_system?f_ri=9534"},{"id":469018,"name":"Neoplasms","url":"https://www.academia.edu/Documents/in/Neoplasms?f_ri=9534"},{"id":559242,"name":"Cardiovascular Diseases","url":"https://www.academia.edu/Documents/in/Cardiovascular_Diseases?f_ri=9534"},{"id":1036777,"name":"Epidemiologic Studies","url":"https://www.academia.edu/Documents/in/Epidemiologic_Studies?f_ri=9534"},{"id":1114577,"name":"Vitamin D Deficiency","url":"https://www.academia.edu/Documents/in/Vitamin_D_Deficiency?f_ri=9534"},{"id":1256683,"name":"Bone and Bones","url":"https://www.academia.edu/Documents/in/Bone_and_Bones?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16938180" data-work_id="16938180" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a 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$(".js-view-count-work_16938180").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="16938180"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">19</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="596" rel="nofollow" href="https://www.academia.edu/Documents/in/Dentistry">Dentistry</a>, <script data-card-contents-for-ri="596" type="text/json">{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="8991" rel="nofollow" href="https://www.academia.edu/Documents/in/Wound_Healing">Wound Healing</a>, <script data-card-contents-for-ri="8991" type="text/json">{"id":8991,"name":"Wound Healing","url":"https://www.academia.edu/Documents/in/Wound_Healing?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="10655" rel="nofollow" href="https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy">Scanning Electron Microscopy</a><script data-card-contents-for-ri="10655" type="text/json">{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16938180]'), work: {"id":16938180,"title":"In vivo evaluation of titanium-prepared platelet-rich fibrin (T-PRF): a new platelet concentrate","created_at":"2015-10-18T02:05:42.585-07:00","url":"https://www.academia.edu/16938180/In_vivo_evaluation_of_titanium_prepared_platelet_rich_fibrin_T_PRF_a_new_platelet_concentrate?f_ri=9534","dom_id":"work_16938180","summary":null,"downloadable_attachments":[{"id":39266890,"asset_id":16938180,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36437596,"first_name":"Mustafa","last_name":"Tunalı","domain_name":"gata","page_name":"MustafaTunalı","display_name":"Mustafa Tunalı","profile_url":"https://gata.academia.edu/MustafaTunal%C4%B1?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=9534","nofollow":true},{"id":8991,"name":"Wound Healing","url":"https://www.academia.edu/Documents/in/Wound_Healing?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":10655,"name":"Scanning Electron Microscopy","url":"https://www.academia.edu/Documents/in/Scanning_Electron_Microscopy?f_ri=9534","nofollow":true},{"id":11404,"name":"Titanium","url":"https://www.academia.edu/Documents/in/Titanium?f_ri=9534"},{"id":61095,"name":"Fibrin","url":"https://www.academia.edu/Documents/in/Fibrin?f_ri=9534"},{"id":65641,"name":"Platelet aggregation","url":"https://www.academia.edu/Documents/in/Platelet_aggregation?f_ri=9534"},{"id":129474,"name":"Connective tissue","url":"https://www.academia.edu/Documents/in/Connective_tissue?f_ri=9534"},{"id":189424,"name":"Blood Coagulation","url":"https://www.academia.edu/Documents/in/Blood_Coagulation?f_ri=9534"},{"id":209508,"name":"Platelet activation mechanism","url":"https://www.academia.edu/Documents/in/Platelet_activation_mechanism?f_ri=9534"},{"id":235677,"name":"Behavioral Animal Models","url":"https://www.academia.edu/Documents/in/Behavioral_Animal_Models?f_ri=9534"},{"id":372622,"name":"Granulation Tissue","url":"https://www.academia.edu/Documents/in/Granulation_Tissue?f_ri=9534"},{"id":385863,"name":"Mouth mucosa","url":"https://www.academia.edu/Documents/in/Mouth_mucosa?f_ri=9534"},{"id":414331,"name":"Centrifugation","url":"https://www.academia.edu/Documents/in/Centrifugation?f_ri=9534"},{"id":606066,"name":"Osteogenesis","url":"https://www.academia.edu/Documents/in/Osteogenesis?f_ri=9534"},{"id":788677,"name":"Rabbits","url":"https://www.academia.edu/Documents/in/Rabbits?f_ri=9534"},{"id":863330,"name":"Osteoblasts","url":"https://www.academia.edu/Documents/in/Osteoblasts?f_ri=9534"},{"id":1031067,"name":"Biocompatible Materials","url":"https://www.academia.edu/Documents/in/Biocompatible_Materials?f_ri=9534"},{"id":1286478,"name":"Osteocytes","url":"https://www.academia.edu/Documents/in/Osteocytes?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_79556110" data-work_id="79556110" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/79556110/The_KSR2_calcineurin_complex_regulates_STIM1_ORAI1_dynamics_and_Store_Operated_Calcium_Entry_SOCE_">The KSR2-calcineurin complex regulates STIM1-ORAI1 dynamics and Store-Operated Calcium Entry (SOCE)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Store-operated calcium entry (SOCE) is the predominant Ca2+ entry mechanism in nonexcitable cells and controls a variety of physiological and pathological processes. Although significant progress has been made in identifying the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_79556110" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Store-operated calcium entry (SOCE) is the predominant Ca2+ entry mechanism in nonexcitable cells and controls a variety of physiological and pathological processes. Although significant progress has been made in identifying the components required for SOCE, the molecular mechanisms underlying it are elusive. The present study provides evidence for a direct involvement of kinase suppressor of Ras 2 (KSR2) in SOCE. Using lymphocytes and fibroblasts from ksr2−/− mice and shKSR2-depleted cells, we find that KSR2 is critical for the elevation of cytosolic Ca2+ concentration. Specifically, our results show that although it is dispensable for Ca2+-store depletion, KSR2 is required for optimal calcium entry. We observe that KSR2 deficiency affects stromal interaction molecule 1 (STIM1)/ORAI1 puncta formation, which is correlated with cytoskeleton disorganization. Of interest, we find that KSR2-associated calcineurin is crucial for SOCE. Blocking calcineurin activity impairs STIM1/ORAI1 pun...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/79556110" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e023d662c4ccc236937e929018542207" rel="nofollow" data-download="{"attachment_id":86231044,"asset_id":79556110,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/86231044/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39321076" href="https://independent.academia.edu/EGiurisato">E. Giurisato</a><script data-card-contents-for-user="39321076" type="text/json">{"id":39321076,"first_name":"E.","last_name":"Giurisato","domain_name":"independent","page_name":"EGiurisato","display_name":"E. Giurisato","profile_url":"https://independent.academia.edu/EGiurisato?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_79556110 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="79556110"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 79556110, container: ".js-paper-rank-work_79556110", }); });</script></li><li class="js-percentile-work_79556110 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 79556110; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_79556110"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_79556110 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="79556110"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 79556110; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=79556110]").text(description); $(".js-view-count-work_79556110").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_79556110").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="79556110"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="7710" rel="nofollow" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="11298" rel="nofollow" href="https://www.academia.edu/Documents/in/Membrane_Proteins">Membrane Proteins</a>, <script data-card-contents-for-ri="11298" type="text/json">{"id":11298,"name":"Membrane Proteins","url":"https://www.academia.edu/Documents/in/Membrane_Proteins?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="13827" rel="nofollow" href="https://www.academia.edu/Documents/in/Cell_Biology">Cell Biology</a><script data-card-contents-for-ri="13827" type="text/json">{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=79556110]'), work: {"id":79556110,"title":"The KSR2-calcineurin complex regulates STIM1-ORAI1 dynamics and Store-Operated Calcium Entry (SOCE)","created_at":"2022-05-20T21:25:53.347-07:00","url":"https://www.academia.edu/79556110/The_KSR2_calcineurin_complex_regulates_STIM1_ORAI1_dynamics_and_Store_Operated_Calcium_Entry_SOCE_?f_ri=9534","dom_id":"work_79556110","summary":"Store-operated calcium entry (SOCE) is the predominant Ca2+ entry mechanism in nonexcitable cells and controls a variety of physiological and pathological processes. Although significant progress has been made in identifying the components required for SOCE, the molecular mechanisms underlying it are elusive. The present study provides evidence for a direct involvement of kinase suppressor of Ras 2 (KSR2) in SOCE. Using lymphocytes and fibroblasts from ksr2−/− mice and shKSR2-depleted cells, we find that KSR2 is critical for the elevation of cytosolic Ca2+ concentration. Specifically, our results show that although it is dispensable for Ca2+-store depletion, KSR2 is required for optimal calcium entry. We observe that KSR2 deficiency affects stromal interaction molecule 1 (STIM1)/ORAI1 puncta formation, which is correlated with cytoskeleton disorganization. Of interest, we find that KSR2-associated calcineurin is crucial for SOCE. Blocking calcineurin activity impairs STIM1/ORAI1 pun...","downloadable_attachments":[{"id":86231044,"asset_id":79556110,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39321076,"first_name":"E.","last_name":"Giurisato","domain_name":"independent","page_name":"EGiurisato","display_name":"E. Giurisato","profile_url":"https://independent.academia.edu/EGiurisato?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":11298,"name":"Membrane Proteins","url":"https://www.academia.edu/Documents/in/Membrane_Proteins?f_ri=9534","nofollow":true},{"id":13827,"name":"Cell Biology","url":"https://www.academia.edu/Documents/in/Cell_Biology?f_ri=9534","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=9534"},{"id":37508,"name":"Cytoskeleton","url":"https://www.academia.edu/Documents/in/Cytoskeleton?f_ri=9534"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=9534"},{"id":57570,"name":"Cercopithecus aethiops","url":"https://www.academia.edu/Documents/in/Cercopithecus_aethiops?f_ri=9534"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=9534"},{"id":99271,"name":"Calcium channels","url":"https://www.academia.edu/Documents/in/Calcium_channels?f_ri=9534"},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus?f_ri=9534"},{"id":422325,"name":"HeLa cells","url":"https://www.academia.edu/Documents/in/HeLa_cells?f_ri=9534"},{"id":609857,"name":"Calcineurin","url":"https://www.academia.edu/Documents/in/Calcineurin?f_ri=9534"},{"id":609860,"name":"NFAT","url":"https://www.academia.edu/Documents/in/NFAT?f_ri=9534"},{"id":868560,"name":"Lymphocytes","url":"https://www.academia.edu/Documents/in/Lymphocytes?f_ri=9534"},{"id":1311259,"name":"Intracellular Space","url":"https://www.academia.edu/Documents/in/Intracellular_Space?f_ri=9534"},{"id":1457054,"name":"Protein Transport","url":"https://www.academia.edu/Documents/in/Protein_Transport?f_ri=9534"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_68476061" data-work_id="68476061" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/68476061/Vitreous_Humor_Biochemical_Constituents_in_Estimation_of_Postmortem_Interval_">Vitreous Humor: Biochemical Constituents in Estimation of Postmortem Interval*,†</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Analysis of biochemical constituents of the vitreous humor can be useful in determining the postmortem interval as there is proportionate postmortem rise of potassium and fall in sodium concentration. We studied 120 autopsy cases to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_68476061" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Analysis of biochemical constituents of the vitreous humor can be useful in determining the postmortem interval as there is proportionate postmortem rise of potassium and fall in sodium concentration. We studied 120 autopsy cases to determine the utility of potassium, sodium, calcium, and chloride levels, and sodium ⁄ potassium ratio in estimating the postmortem interval. There was a linear relationship between vitreous potassium concentration and postmortem interval, whereas an inverse relationship between vitreous sodium ⁄ potassium ratio and postmortem interval was noted. Other factors like age, sex, cause of death, season of death, and refrigeration of sample did not influence the vitreous humor potassium values. Using the statistical tools, a new formula was derived to determine the postmortem interval based on the potassium concentration and a review of previous literature is presented. Hence, the findings of this study supported a central role of vitreous humor biochemistry in many postmortem forensic and pathological evaluations.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/68476061" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c4b0cc7a237245165b649336f9b0d1db" rel="nofollow" data-download="{"attachment_id":78937974,"asset_id":68476061,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/78937974/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="41174107" href="https://independent.academia.edu/KusumJashnani">Kusum Jashnani</a><script data-card-contents-for-user="41174107" type="text/json">{"id":41174107,"first_name":"Kusum","last_name":"Jashnani","domain_name":"independent","page_name":"KusumJashnani","display_name":"Kusum Jashnani","profile_url":"https://independent.academia.edu/KusumJashnani?f_ri=9534","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_68476061 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="68476061"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 68476061, container: ".js-paper-rank-work_68476061", }); 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$(".js-view-count[data-work-id=68476061]").text(description); $(".js-view-count-work_68476061").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_68476061").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="68476061"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1588" rel="nofollow" href="https://www.academia.edu/Documents/in/Forensic_Science">Forensic Science</a>, <script data-card-contents-for-ri="1588" type="text/json">{"id":1588,"name":"Forensic Science","url":"https://www.academia.edu/Documents/in/Forensic_Science?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="9534" rel="nofollow" href="https://www.academia.edu/Documents/in/Calcium">Calcium</a>, <script data-card-contents-for-ri="9534" type="text/json">{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="21717" rel="nofollow" href="https://www.academia.edu/Documents/in/Forensic_Pathology">Forensic Pathology</a>, <script data-card-contents-for-ri="21717" type="text/json">{"id":21717,"name":"Forensic Pathology","url":"https://www.academia.edu/Documents/in/Forensic_Pathology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" rel="nofollow" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a><script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=68476061]'), work: {"id":68476061,"title":"Vitreous Humor: Biochemical Constituents in Estimation of Postmortem Interval*,†","created_at":"2022-01-16T22:44:34.426-08:00","url":"https://www.academia.edu/68476061/Vitreous_Humor_Biochemical_Constituents_in_Estimation_of_Postmortem_Interval_?f_ri=9534","dom_id":"work_68476061","summary":"Analysis of biochemical constituents of the vitreous humor can be useful in determining the postmortem interval as there is proportionate postmortem rise of potassium and fall in sodium concentration. We studied 120 autopsy cases to determine the utility of potassium, sodium, calcium, and chloride levels, and sodium ⁄ potassium ratio in estimating the postmortem interval. There was a linear relationship between vitreous potassium concentration and postmortem interval, whereas an inverse relationship between vitreous sodium ⁄ potassium ratio and postmortem interval was noted. Other factors like age, sex, cause of death, season of death, and refrigeration of sample did not influence the vitreous humor potassium values. Using the statistical tools, a new formula was derived to determine the postmortem interval based on the potassium concentration and a review of previous literature is presented. Hence, the findings of this study supported a central role of vitreous humor biochemistry in many postmortem forensic and pathological evaluations.","downloadable_attachments":[{"id":78937974,"asset_id":68476061,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41174107,"first_name":"Kusum","last_name":"Jashnani","domain_name":"independent","page_name":"KusumJashnani","display_name":"Kusum Jashnani","profile_url":"https://independent.academia.edu/KusumJashnani?f_ri=9534","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":1588,"name":"Forensic Science","url":"https://www.academia.edu/Documents/in/Forensic_Science?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534","nofollow":true},{"id":21717,"name":"Forensic Pathology","url":"https://www.academia.edu/Documents/in/Forensic_Pathology?f_ri=9534","nofollow":true},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=9534","nofollow":true},{"id":24246,"name":"FORENSIC","url":"https://www.academia.edu/Documents/in/FORENSIC?f_ri=9534"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=9534"},{"id":26991,"name":"Sciences","url":"https://www.academia.edu/Documents/in/Sciences?f_ri=9534"},{"id":28850,"name":"Linear models","url":"https://www.academia.edu/Documents/in/Linear_models?f_ri=9534"},{"id":62112,"name":"Prospective studies","url":"https://www.academia.edu/Documents/in/Prospective_studies?f_ri=9534"},{"id":66408,"name":"Forensic Sciences","url":"https://www.academia.edu/Documents/in/Forensic_Sciences?f_ri=9534"},{"id":93433,"name":"Photometry","url":"https://www.academia.edu/Documents/in/Photometry?f_ri=9534"},{"id":160656,"name":"Potassium","url":"https://www.academia.edu/Documents/in/Potassium?f_ri=9534"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=9534"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=9534"},{"id":2260515,"name":"Chlorides","url":"https://www.academia.edu/Documents/in/Chlorides?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_65412471" data-work_id="65412471" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/65412471/Biohybrid_microsystems_actuated_by_cardiomyocytes_microcantilever_microrobot_and_micropump">Biohybrid microsystems actuated by cardiomyocytes: microcantilever, microrobot, and micropump</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This paper introduces biohybrid microsystems actuated by cardiomyocytes, such as microcantilever, microrobot, and micropump. The microfabicated biohybrid microcantilever can measure the contractile force of self-organized cardiomyocytes.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_65412471" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This paper introduces biohybrid microsystems actuated by cardiomyocytes, such as microcantilever, microrobot, and micropump. The microfabicated biohybrid microcantilever can measure the contractile force of self-organized cardiomyocytes. The microcantilever is made of a biocompatible PDMS substrate, using a simple microfabrication technique and a specially designed 3D micromolding aligner. The contractile force of the cardiomyocytes makes a bending displacement of the microcantilever. Conversely, from the displacement, we can estimate the contractile of the cardiomyocytes on the microcantilever. The bending motion of the microcantilever can be applied as a biohybrid actuator of microrobot. We have developed a novel method to fabricate a crab-like microrobot that can actuate for a long period under a physiological condition. The microrobot consists of three separate front and rear legs which have a shape of the microcantilever. The performance of our crab-like microrobot was measured at an average velocity of 100 μ m/s, and the estimated total distance it traveled was 50 m for a one-week period. Finally, a micropump actuated by the self-beating cardiomyocytes is fabricated and the pumping performance is demonstrated.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/65412471" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2585a1910dca131f888fcf2f0caa00d1" rel="nofollow" data-download="{"attachment_id":77021355,"asset_id":65412471,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/77021355/download_file?st=MTc0MTA3MTEyOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="24169492" href="https://independent.academia.edu/JungyulPark">Jungyul Park</a><script data-card-contents-for-user="24169492" type="text/json">{"id":24169492,"first_name":"Jungyul","last_name":"Park","domain_name":"independent","page_name":"JungyulPark","display_name":"Jungyul Park","profile_url":"https://independent.academia.edu/JungyulPark?f_ri=9534","photo":"https://0.academia-photos.com/24169492/9015091/10057887/s65_jungyul.park.jpg"}</script></span></span></li><li class="js-paper-rank-work_65412471 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="65412471"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 65412471, container: ".js-paper-rank-work_65412471", }); 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$(".js-view-count[data-work-id=65412471]").text(description); $(".js-view-count-work_65412471").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_65412471").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="65412471"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="422" rel="nofollow" href="https://www.academia.edu/Documents/in/Computer_Science">Computer Science</a>, <script data-card-contents-for-ri="422" type="text/json">{"id":422,"name":"Computer Science","url":"https://www.academia.edu/Documents/in/Computer_Science?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="511" rel="nofollow" href="https://www.academia.edu/Documents/in/Materials_Science">Materials Science</a>, <script data-card-contents-for-ri="511" type="text/json">{"id":511,"name":"Materials Science","url":"https://www.academia.edu/Documents/in/Materials_Science?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="606" rel="nofollow" href="https://www.academia.edu/Documents/in/Cardiology">Cardiology</a>, <script data-card-contents-for-ri="606" type="text/json">{"id":606,"name":"Cardiology","url":"https://www.academia.edu/Documents/in/Cardiology?f_ri=9534","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="5467" rel="nofollow" href="https://www.academia.edu/Documents/in/Biomimetics">Biomimetics</a><script data-card-contents-for-ri="5467" type="text/json">{"id":5467,"name":"Biomimetics","url":"https://www.academia.edu/Documents/in/Biomimetics?f_ri=9534","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=65412471]'), work: {"id":65412471,"title":"Biohybrid microsystems actuated by cardiomyocytes: microcantilever, microrobot, and micropump","created_at":"2021-12-21T22:12:51.516-08:00","url":"https://www.academia.edu/65412471/Biohybrid_microsystems_actuated_by_cardiomyocytes_microcantilever_microrobot_and_micropump?f_ri=9534","dom_id":"work_65412471","summary":"This paper introduces biohybrid microsystems actuated by cardiomyocytes, such as microcantilever, microrobot, and micropump. The microfabicated biohybrid microcantilever can measure the contractile force of self-organized cardiomyocytes. The microcantilever is made of a biocompatible PDMS substrate, using a simple microfabrication technique and a specially designed 3D micromolding aligner. The contractile force of the cardiomyocytes makes a bending displacement of the microcantilever. Conversely, from the displacement, we can estimate the contractile of the cardiomyocytes on the microcantilever. The bending motion of the microcantilever can be applied as a biohybrid actuator of microrobot. We have developed a novel method to fabricate a crab-like microrobot that can actuate for a long period under a physiological condition. The microrobot consists of three separate front and rear legs which have a shape of the microcantilever. The performance of our crab-like microrobot was measured at an average velocity of 100 μ m/s, and the estimated total distance it traveled was 50 m for a one-week period. Finally, a micropump actuated by the self-beating cardiomyocytes is fabricated and the pumping performance is demonstrated.","downloadable_attachments":[{"id":77021355,"asset_id":65412471,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":24169492,"first_name":"Jungyul","last_name":"Park","domain_name":"independent","page_name":"JungyulPark","display_name":"Jungyul Park","profile_url":"https://independent.academia.edu/JungyulPark?f_ri=9534","photo":"https://0.academia-photos.com/24169492/9015091/10057887/s65_jungyul.park.jpg"}],"research_interests":[{"id":422,"name":"Computer Science","url":"https://www.academia.edu/Documents/in/Computer_Science?f_ri=9534","nofollow":true},{"id":511,"name":"Materials Science","url":"https://www.academia.edu/Documents/in/Materials_Science?f_ri=9534","nofollow":true},{"id":606,"name":"Cardiology","url":"https://www.academia.edu/Documents/in/Cardiology?f_ri=9534","nofollow":true},{"id":5467,"name":"Biomimetics","url":"https://www.academia.edu/Documents/in/Biomimetics?f_ri=9534","nofollow":true},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium?f_ri=9534"},{"id":10583,"name":"Motion control","url":"https://www.academia.edu/Documents/in/Motion_control?f_ri=9534"},{"id":57186,"name":"Actuators","url":"https://www.academia.edu/Documents/in/Actuators?f_ri=9534"},{"id":76608,"name":"Microfabrication","url":"https://www.academia.edu/Documents/in/Microfabrication?f_ri=9534"},{"id":184772,"name":"Cantilevers","url":"https://www.academia.edu/Documents/in/Cantilevers?f_ri=9534"},{"id":205996,"name":"Micropump","url":"https://www.academia.edu/Documents/in/Micropump?f_ri=9534"},{"id":212372,"name":"Bending","url":"https://www.academia.edu/Documents/in/Bending?f_ri=9534"},{"id":279495,"name":"Robustness","url":"https://www.academia.edu/Documents/in/Robustness?f_ri=9534"},{"id":299563,"name":"Self Organization","url":"https://www.academia.edu/Documents/in/Self_Organization?f_ri=9534"},{"id":462777,"name":"Leg","url":"https://www.academia.edu/Documents/in/Leg?f_ri=9534"}]}, }) } })();</script></ul></li></ul></div></div></div><div class="u-taCenter Pagination"><ul 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