Human Pro-MMP-13 ELISA - Quantikine DM1300: R&D Systems

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class="ds_title">Human Pro-MMP-13 ELISA Kit - Quantikine </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: DM1300</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 34 Citations </a> </span> <span class="reviews review_stars_5"> <a href="#product-reviews" id="product_reviews" class="jump_to" data-tab="6" data-tabname="product-reviews"> 2 Reviews </a> </span> <a href="/reviews/DM1300" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/human-pro-mmp-13-quantikine-elisa-kit_dm1300" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog #</th> <th class="ds_availability_column mobile-hide">Availability</th> <th class="ds_size_price_column">Size / Price</th> <th class="ds_quantity_column">Qty</th> </tr> </thead> <tbody> <tr class="et_atc_product" data-sku="PDM1300"> <td class="dat_catalog_column">PDM1300</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity ds_quantity_column"><div class="form-item form-item-catnum-pdm1300 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-pdm1300" name="catnum-PDM1300" value="" size="5" maxlength="3" /></div></td> </tr> <tr class="et_atc_product" data-sku="SMP1300"> <td class="dat_catalog_column">SMP1300</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity 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class="additional_product_label"><a href="/products/quantikine-immunoassay-control-set-1180-for-human-pro-mmp-13_qc132">QC132: Quantikine Immunoassay Control Set 1180 for Human Pro-MMP-13</a></div><div class="et_atc_results"></div><div class="form-item form-item-catnum-qc132 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-qc132" name="catnum-QC132" value="" size="5" maxlength="3" /></div></div></div> <div class="ds_atc_pre_buttons"></div> <div class="ds_atc_buttons"> <a href="/quote/instruments/ella-automated-immunoassay-system" class="btn btn-secondary atc_request_quote_simple_plex_assay" style="display:none" rel="nofollow">Request a Quote</a> <a href="/services/bulk-quote-request?catnum=DM1300&product=Human Pro-MMP-13 Quantikine ELISA Kit" class="btn btn-secondary atc_hidden bulk_btn" style="display:none;" rel="nofollow" title="Competitive discounts on products purchased in bulk quantities">Bulk Orders</a> <button class="btn btn-primary atc_hidden atc_btn form-submit icon-before" style="display:none;" title="Enter quantities above and click to add to cart" type="submit" id="edit-add-to-cart" name="add_to_cart_btn" value="Add to Cart"><span class="icon glyphicon glyphicon-plus" aria-hidden="true"></span> Add to Cart</button> </div> <div class="ds_atc_post_buttons"></div> </div> <input type="hidden" name="form_build_id" value="form-ClimMcGJbP2N3vWwjD7gadHsZnghW-MC0yDreXsT-N0" /> <input type="hidden" name="form_id" value="atc_datasheet_form" /> </div></form> </div> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="Human Pro-MMP-13 ELISA Standard Curve" data-footer="Human Pro-MMP-13 ELISA Standard Curve" title="Cat. No. DM1300 - Human Pro-MMP-13 Quantikine ELISA Kit" href="https://resources.rndsystems.com/images/datasheets/elisa/DATA_MMP13_DM1300_ELISA_751.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/elisa/DATA_MMP13_DM1300_ELISA_751.jpg" class="img-fluid" alt="Human Pro-MMP-13 ELISA Standard Curve" title="Human Pro-MMP-13 ELISA Standard Curve"> </a> <div class="search_image_other_count"> <span class="search_more_images">2 Images</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="Human Pro-MMP-13 ELISA Linearity" data-title="Human Pro-MMP-13 ELISA Linearity" title="Cat. No. DM1300 - Human Pro-MMP-13 Quantikine ELISA Kit" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/elisa/MMP-13_DM1300_ELISA_136.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/elisa/MMP-13_DM1300_ELISA_136.jpg" class="img-fluid" loading="lazy" alt="Human Pro-MMP-13 ELISA Linearity" title="Human Pro-MMP-13 ELISA Linearity"> </a> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/mmp-13?category=ELISAs" class="btn btn-secondary">All MMP-13 ELISAs</a> </div> </div> <div class="tabbed_content" id="rnd_elisa"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_2" data-tab="2" data-tabname="assay-procedure" id="dsTabs_assay-procedure"> <span>Procedure</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (34)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews (2) </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_precision" id="product-anchor-ds_precision">Precision</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_recovery" id="product-anchor-ds_recovery">Recovery</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_linearity" id="product-anchor-ds_linearity">Linearity</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <div id="ds_summary" class="datasheet_section"> <h2>Human Pro-MMP-13 Quantikine ELISA Kit Summary</h2> <div class="ds_table summary_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Type</div> <div class="ds_table_cell summary_table_data">Solid Phase Sandwich ELISA</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Format</div> <div class="ds_table_cell summary_table_data">96-well strip plate</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Length</div> <div class="ds_table_cell summary_table_data">4.5 hours</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sample Type & Volume Required Per Well</div> <div class="ds_table_cell summary_table_data">Cell Culture Supernates (50 uL)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sensitivity</div> <div class="ds_table_cell summary_table_data">21.3 pg/mL</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Range</div> <div class="ds_table_cell summary_table_data">78.0 - 5,000 pg/mL (Cell Culture Supernates)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data">Natural and recombinant human pro-MMP-13</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Cross-reactivity</div> <div class="ds_table_cell summary_table_data">< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Interference</div> <div class="ds_table_cell summary_table_data">No significant interference observed with available related molecules.</div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="DM1300"></div> <div class="datasheet_section"> <h2>Product Summary</h2> <div> The Quantikine Human Pro-MMP-13 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure pro-MMP-13 <span>in cell culture supernates. It contains NS0-expressed recombinant human pro-MMP-13 and antibodies raised against the recombinant factor. The Quantikine kit will not detect recombinant human active MMP-13 existing in either free or TIMP-bound form. Natural human pro-MMP-13 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human pro-MMP-13.</span> </div> </div> <div id="ds_precision" class="datasheet_section"> <h2>Precision</h2> <div><strong>Intra-Assay Precision (Precision within an assay)</strong> Three samples of known concentration were tested on one plate to assess intra-assay precision</div><div><strong>Inter-Assay Precision (Precision between assays)</strong> Three samples of known concentration were tested in separate assays to assess inter-assay precision</div> <p class="precision_title"><strong>Cell Culture Supernates</strong></p> <table class="ds_table_html precision_table"> <thead> <tr> <th class="p1"></th> <th colspan="3">Intra-Assay Precision</th> <th colspan="3">Inter-Assay Precision</th> </tr> </thead> <tbody> <tr> <td><strong>Sample</strong></td> <td>1</td> <td>2</td> <td>3</td> <td>1</td> <td>2</td> <td>3</td> </tr> <tr> <td><strong>n</strong></td> <td>20</td> <td>20</td> <td>20</td> <td>40</td> <td>40</td> <td>40</td> </tr> <tr> <td><strong>Mean (pg/mL)</strong></td> <td>485</td> <td>1462</td> <td>3521</td> <td>533</td> <td>1561</td> <td>3823</td> </tr> <tr> <td><strong>Standard Deviation</strong></td> <td>18.2</td> <td>64</td> <td>93</td> <td>27.9</td> <td>56.1</td> <td>143</td> </tr> <tr> <td><strong>CV%</strong></td> <td>3.8</td> <td>4.4</td> <td>2.6</td> <td>5.2</td> <td>3.6</td> <td>3.7</td> </tr> </tbody> </table><br/> </div> <div id="ds_recovery" class="datasheet_section"> <h2>Recovery</h2> <div> <p>The recovery of pro-MMP-13 spiked to levels throughout the range of the assay was evaluated.<p> </div> <table class="ds_table_html recovery_table"> <thead> <th>Sample Type</th> <th>Average % Recovery</th> <th>Range %</th> </thead> <tr> <td>Cell Culture Media (n=2)</td> <td>101</td> <td>86-112</td> </tr> <tr> <td>Cell Culture Supernates (n=3)</td> <td>98</td> <td>85-111</td> </tr> </table> </div> <div id="ds_linearity" class="datasheet_section"> <h2>Linearity</h2> <div>To assess the linearity of the assay, samples containing or spiked with high concentrations of pro-MMP-13 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.</div> <div> <img class=" hideoverflow quantikineLinearityImage" alt="Human Pro-MMP-13 ELISA Linearity" src="https://resources.rndsystems.com/images/datasheets/elisa/MMP-13_DM1300_ELISA_136.jpg"> </div> <div class="marginBottom"></div> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> N/A </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Human Pro-MMP-13 ELISA Standard Curve" data-footer="Human Pro-MMP-13 ELISA Standard Curve" title="Cat. No. - Human Pro-MMP-13 ELISA Standard Curve" href="https://resources.rndsystems.com/images/datasheets/elisa/DATA_MMP13_DM1300_ELISA_751.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/elisa/DATA_MMP13_DM1300_ELISA_751.jpg" class="img-fluid" alt="Human Pro-MMP-13 ELISA Standard Curve" title="Human Pro-MMP-13 ELISA Standard Curve"> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Human Pro-MMP-13 ELISA Standard Curve</span> </p> </div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/dm1300.pdf?v=20241126" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-pro-mmp-13-quantikine-elisa-kit_dm1300">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//resources.rndsystems.com/pdfs/msds/dm1300.pdf?v=20241126">SDS</a> </div> </div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Storage</div> <div class="ds_table_cell prep_table_data">Store the unopened product at 2 - 8 °C. Do not use past expiration date. </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: MMP-13</h2> <p><P>The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP). </P></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name:</div> <div class="ds_table_cell background_table_data">Matrix Metalloproteinase 13</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs:</div> <div class="ds_table_cell background_table_data">4322 (Human)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names:</div> <div class="ds_table_cell background_table_data">CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC 3.4.24.22; EC 3.4.24.24; EC 3.4.24.35; EC 3.4.24.65; EC 3.4.24.7; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13</div> </div> </div> </div> </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/cancer-biomarkers">Cancer Biomarkers</a> </li> <li> <a href="/research-area/collagen-modifying-enzymes">Collagen Modifying Enzymes</a> </li> <li> <a href="/research-area/extracellular-matrix-remodeling-and-cell-migration-during-emt">Extracellular Matrix Remodeling and Cell Migration During EMT</a> </li> <li> <a href="/research-area/metalloproteases-and-regulators">Metalloproteases and Regulators</a> </li> <li> <a href="/research-area/mmps--timps-and-related-molecules">MMPs, TIMPs and Related Molecules</a> </li> <li> <a href="/research-area/search-bioprocessing-enzymes">Search Bioprocessing Enzymes</a> </li> </ul> </div> <div class="prop_65_warning"><div class="prop_65_warning">&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.</div></div> </div> <div class="tab_content tab_2" data-tab="2" data-tabname="product-assay-procedure" id="product-assay-procedure"> <a id="assay-procedure"></a> <div id="boxes">  <h2>Assay Procedure</h2> <strong>Refer to the product <datasheet> for complete assay procedure.</strong><br /><br /> <div class="psp_procedure_header"> Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate. </div> <ol class="psp_procedure"> <li><span>  Prepare all reagents, standard dilutions, and samples as directed in the product insert.</span></li> <li><span>  Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>100 µL Assay Diluent</strong><br /><img src="//resources.rndsystems.com/images/site/wells-plain.png" align="right" /></div> <li><span>  Add 100 µL of Assay Diluent to each well.</span> </li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong class="AssayStepMultiLine">50 µL Standard, Control, or Sample</strong><br /><img src="//resources.rndsystems.com/images/site/wells-plain.png" align="right" /></div> <li><span>  Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.</span> </li> <li><span>  Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>200 µL Conjugate</strong><br /><img src="//resources.rndsystems.com/images/site/wells-plain.png" align="right" /></div> <li><span>  Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.</span></li> <li><span>  Aspirate and wash 4 times.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>200 µL Substrate Solution</strong><br /><img src="//resources.rndsystems.com/images/site/wells-blue.png" align="right" /></div> <li><span>  Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.</span></li> <hr class="psp_procedure" /> <div class="psp_assayimage"><strong>50 µL Stop Solution</strong><br /><img src="//resources.rndsystems.com/images/site/wells-yellow.png" align="right" /></div> <li><span>  Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.</span></li> </ol> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Human Pro-MMP-13 Quantikine ELISA Kit</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">34</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">10</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Guinea Pig"> Guinea Pig </option> <option class="citation_filterRadio" value="species" data-filter="Human"> Human </option> <option class="citation_filterRadio" value="species" data-filter="Rat"> Rat </option> </select> </div> <input type="hidden" id="selAntibody" value="All"> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Cell Culture Supernates"> Cell Culture Supernates </option> <option class="citation_filterRadio" value="sampletype" data-filter="Cell Culture Supernates, Synovial Fluid"> Cell Culture Supernates, Synovial Fluid </option> <option class="citation_filterRadio" value="sampletype" data-filter="Edema Fluid"> Edema Fluid </option> <option class="citation_filterRadio" value="sampletype" data-filter="Ovarian Ascites Fluid"> Ovarian Ascites Fluid </option> <option class="citation_filterRadio" value="sampletype" data-filter="Plasma"> Plasma </option> <option class="citation_filterRadio" value="sampletype" data-filter="Serum"> Serum </option> <option class="citation_filterRadio" value="sampletype" data-filter="Synovial Fluid"> Synovial Fluid </option> <option class="citation_filterRadio" value="sampletype" data-filter="Synovial Lavage Fluid"> Synovial Lavage Fluid </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="34" data-max-show="10"> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates, Synovial Fluid"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/39103466"> Catabolic mediators from TLR2-mediated proteoglycan aggrecan peptide-stimulated chondrocytes are reduced by Lactobacillus-conditioned media </a> </span><br/> Authors: Sengprasert, P;Waitayangkoon, P;Kamenkit, O;Sawatpanich, A;Chaichana, T;Wongphoom, J;Ngarmukos, S;Taweevisit, M;Lotinun, S;Tumwasorn, S;Tanavalee, A;Reantragoon, R;<br/> Scientific reports<br/> Species: Human<br/> Sample Types: Cell Culture Supernates, Synovial Fluid<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35624126"> Adipokine human Resistin promotes obesity-associated inflammatory intervertebral disc degeneration via pro-inflammatory cytokine cascade activation </a> </span><br/> Authors: JH Shin, S Park, H Cho, JH Kim, H Choi<br/> Scientific Reports, 2022-05-27;12(1):8936.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35329213"> Concentration of Selected Metalloproteinases and Osteocalcin in the Serum and Synovial Fluid of Obese Women with Advanced Knee Osteoarthritis </a> </span><br/> Authors: J Jarecki, T Ma?ecka-Ma, E Kosior-Jar, W Widuchowsk, P Krasowski, M Gutbier, M Dobrzy?ski, T Blicharski<br/> International Journal of Environmental Research and Public Health, 2022-03-16;19(6):.<br/> Species: Human<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/34440671"> An In Vitro System to Study the Effect of Subchondral Bone Health on Articular Cartilage Repair in Humans </a> </span><br/> Authors: T Hopkins, KT Wright, NJ Kuiper, S Roberts, P Jermin, P Gallacher, JH Kuiper<br/> Cells, 2021-07-27;10(8):.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/32015418"> Inhibition of Serine Protease Activity Protects Against High Fat Diet-Induced Inflammation and Insulin Resistance </a> </span><br/> Authors: CS Kuo, JS Chen, LY Lin, GW Schmid-Sch, S Chien, PH Huang, JW Chen, SJ Lin<br/> Sci Rep, 2020-02-03;10(1):1725.<br/> Species: Human<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/30728324"> Hypomorphic mutations of TRIP11 cause odontochondrodysplasia </a> </span><br/> Authors: A Wehrle, TM Witkos, S Unger, J Schneider, JA Follit, J Hermann, T Welting, V Fano, M Hietala, N Vatanavich, K Schoner, J Spranger, M Schmidts, B Zabel, GJ Pazour, A Bloch-Zupa, G Nishimura, A Superti-Fu, M Lowe, E Lausch<br/> JCI Insight, 2019-02-07;4(3):.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Guinea Pig" data-application="" data-sampletype="Synovial Lavage Fluid"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/29284451"> Synovial inflammation plays a greater role in post-traumatic osteoarthritis compared to idiopathic osteoarthritis in the Hartley guinea pig knee </a> </span><br/> Authors: NP Thomas, WJ Wu, BC Fleming, F Wei, Q Chen, L Wei<br/> BMC Musculoskelet Disord, 2017-12-29;18(1):556.<br/> Species: Guinea Pig<br/> Sample Types: Synovial Lavage Fluid<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/28873368"> Krï¿½ppel-Like Factor 2 Regulates Degradation of Type II Collagen by Suppressing the Expression of Matrix Metalloproteinase (MMP)-13 </a> </span><br/> Authors: Y Yuan, H Tan, P Dai<br/> Cell. Physiol. Biochem., 2017-08-15;42(6):2159-2168.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/28526405"> Hypoxia-inducible factor-l? mediates aggrecan and collagen ? expression via NOTCH1 signaling in nucleus pulposus cells during intervertebral disc degeneration </a> </span><br/> Authors: Z Liu, C Li, X Meng, Y Bai, J Qi, J Wang, Q Zhou, W Zhang, X Zhang<br/> Biochem. Biophys. Res. Commun., 2017-05-17;0(0):.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/28521450"> Atmospheric particulate matter2.5 promotes the migration and invasion of hepatocellular carcinoma cells </a> </span><br/> Authors: Q Zhang, Q Luo, X Yuan, L Chai, D Li, J Liu, Z Lv<br/> Oncol Lett, 2017-03-29;13(5):3445-3450.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <div id="ds_extra_cits"> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/27982117"> Galectin-3 Induces a Pro-degradative/inflammatory Gene Signature in Human Chondrocytes, Teaming Up with Galectin-1 in Osteoarthritis Pathogenesis </a> </span><br/> Sci Rep, 2016-12-16;6(0):39112.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/22662110"> Central role of SREBP-2 in the pathogenesis of osteoarthritis. </a> </span><br/> Authors: Kostopoulou F, Gkretsi V, Malizos KN, Iliopoulos D, Oikonomou P, Poultsides L, Tsezou A<br/> PLoS ONE, 2012-05-25;7(5):e35753.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/22359588"> Anti-arthritic effects of magnolol in human interleukin 1ÃŽÂ²-stimulated fibroblast-like synoviocytes and in a rat arthritis model. </a> </span><br/> Authors: Wang JH, Shih KS, Liou JP, Wu YW, Chang AS, Wang KL, Tsai CL, Yang CR<br/> PLoS ONE, 2012-02-16;7(2):e31368.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Edema Fluid"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/21257730"> Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells. </a> </span><br/> Authors: Yamakawa N, Uchida T, Matthay MA, Makita K<br/> Am. J. Physiol. Lung Cell Mol. Physiol., 2011-01-21;300(4):L516-25.<br/> Species: Human<br/> Sample Types: Edema Fluid<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/21059338"> High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1. </a> </span><br/> Authors: Hashizume M, Mihara M<br/> Biochem. Biophys. Res. Commun., 2010-11-06;403(2):184-9.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/19922462"> Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation. </a> </span><br/> Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O<br/> Eur. J. Haematol., 2009-11-17;84(3):239-51.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/19714628"> Establishment of a matrix-associated transepithelial resistance invasion assay to precisely measure the invasive potential of synovial fibroblasts. </a> </span><br/> Authors: Wunrau C, Schnaeker EM, Freyth K, Pundt N, Wendholt D, Neugebauer K, Hansen U, Pap T, Dankbar B<br/> Arthritis Rheum., 2009-09-01;60(9):2606-11.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/19248116"> Interleukin-7 stimulates secretion of S100A4 by activating the JAK/STAT signaling pathway in human articular chondrocytes. </a> </span><br/> Authors: Yammani RR, Long D, Loeser RF<br/> Arthritis Rheum., 2009-03-01;60(3):792-800.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/18975308"> Inhibition of interleukin-1beta-induced matrix metalloproteinases 1 and 13 production in human osteoarthritic chondrocytes by prostaglandin D2. </a> </span><br/> Authors: Zayed N, Afif H, Chabane N, Mfuna-Endam L, Benderdour M, Martel-Pelletier J, Pelletier JP, Motiani RK, Trebak M, Duval N, Fahmi H<br/> Arthritis Rheum., 2008-11-01;58(11):3530-40.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/18802112"> Prostaglandin E2 exerts catabolic effects in osteoarthritis cartilage: evidence for signaling via the EP4 receptor. </a> </span><br/> Authors: Attur M, Al-Mussawir HE, Patel J, Kitay A, Dave M, Palmer G, Pillinger MH, Abramson SB<br/> J. Immunol., 2008-10-01;181(7):5082-8.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Ovarian Ascites Fluid"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/18790755"> Targeting a metalloprotease-PAR1 signaling system with cell-penetrating pepducins inhibits angiogenesis, ascites, and progression of ovarian cancer. </a> </span><br/> Authors: Agarwal A, Covic L, Sevigny LM, Kaneider NC, Lazarides K, Azabdaftari G, Sharifi S, Kuliopulos A<br/> Mol. Cancer Ther., 2008-09-01;7(9):2746-57.<br/> Species: Human<br/> Sample Types: Ovarian Ascites Fluid<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17618483"> Amidic alginate hydrogel for nucleus pulposus replacement. </a> </span><br/> Authors: Leone G, Torricelli P, Chiumiento A, Facchini A, Barbucci R<br/> J Biomed Mater Res A, 2008-02-01;84(2):391-401.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/18022226"> Alendronate-hydroxyapatite nanocomposites and their interaction with osteoclasts and osteoblast-like cells. </a> </span><br/> Authors: Boanini E, Torricelli P, Gazzano M, Giardino R, Bigi A<br/> Biomaterials, 2007-11-19;29(7):790-6.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human, Rat" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17876296"> ROCK-II mediates colon cancer invasion via regulation of MMP-2 and MMP-13 at the site of invadopodia as revealed by multiphoton imaging. </a> </span><br/> Authors: Vishnubhotla R, Sun S, Huq J, Bulic M, Ramesh A, Guzman G, Cho M, Glover SC<br/> Lab. Invest., 2007-09-17;87(11):1149-58.<br/> Species: Human, Rat<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17599750"> Calcineurin modulates the catabolic and anabolic activity of chondrocytes and participates in the progression of experimental osteoarthritis. </a> </span><br/> Authors: Yoo SA, Park BH, Yoon HJ, Lee JY, Song JH, Kim HA, Cho CS, Kim WU<br/> Arthritis Rheum., 2007-07-01;56(7):2299-311.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17434489"> Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes. </a> </span><br/> Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK<br/> FEBS Lett., 2007-04-09;581(9):1928-32.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Synovial Fluid"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17311929"> Basic fibroblast growth factor stimulates matrix metalloproteinase-13 via the molecular cross-talk between the mitogen-activated protein kinases and protein kinase Cdelta pathways in human adult articular chondrocytes. </a> </span><br/> Authors: Im HJ, Muddasani P, Natarajan V, Schmid TM, Block JA, Davis F, van Wijnen AJ, Loeser RF<br/> J. Biol. Chem., 2007-02-20;282(15):11110-21.<br/> Species: Human<br/> Sample Types: Synovial Fluid<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/17009243"> Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures. </a> </span><br/> Authors: Fearon U, Mullan R, Markham T, Connolly M, Sullivan S, Poole AR, FitzGerald O, Bresnihan B, Veale DJ<br/> Arthritis Rheum., 2006-10-01;54(10):3152-62.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/16948116"> Increase in production of matrix metalloproteinase 13 by human articular chondrocytes due to stimulation with S100A4: Role of the receptor for advanced glycation end products. </a> </span><br/> Authors: Yammani RR, Carlson CS, Bresnick AR, Loeser RF<br/> Arthritis Rheum., 2006-09-01;54(9):2901-11.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/16547285"> The potential of adiponectin in driving arthritis. </a> </span><br/> Authors: Ehling A, Schaffler A, Herfarth H, Tarner IH, Anders S, Distler O, Paul G, Distler J, Gay S, Scholmerich J, Neumann E, Muller-Ladner U<br/> J. Immunol., 2006-04-01;176(7):4468-78.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/16377621"> The role of human HtrA1 in arthritic disease. </a> </span><br/> Authors: Grau S, Richards PJ, Kerr B, Hughes C, Caterson B, Williams AS, Junker U, Jones SA, Clausen T, Ehrmann M<br/> J. Biol. Chem., 2005-12-22;281(10):6124-9.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Synovial Fluid"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/15618147"> Induction of host matrix metalloproteinases by Borrelia burgdorferi differs in human and murine lyme arthritis. </a> </span><br/> Authors: Behera AK, Hildebrand E, Scagliotti J, Steere AC, Hu LT<br/> Infect. Immun., 2005-01-01;73(1):126-34.<br/> Species: Human<br/> Sample Types: Synovial Fluid<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/15557436"> Stimulation of matrix metalloproteinases by hyperosmolarity via a JNK pathway in human corneal epithelial cells. </a> </span><br/> Authors: Li DQ, Chen Z, Song XJ, Luo L, Pflugfelder SC<br/> Invest. Ophthalmol. Vis. Sci., 2004-12-01;45(12):4302-11.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/15292257"> Differential modulation of human melanoma cell metalloproteinase expression by alpha2beta1 integrin and CD44 triple-helical ligands derived from type IV collagen. </a> </span><br/> Authors: Baronas-Lowell D, Lauer-Fields JL, Borgia JA, Sferrazza GF, Al-Ghoul M, Minond D, Fields GB<br/> J. Biol. Chem., 2004-08-02;279(42):43503-13.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> </div> </ol> </div> <div class="clearfix"><button class="btn btn-quinary ds_extra_cits pull-right toggle_cits_down" id="toggle_cits"></button></div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/elisas"> View all ELISA FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="dm1300" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" 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Human Pro-MMP-13 ELISA - Quantikine DM1300: R&D Systems