Substrate Reagent Pack (DY999) by R&D Systems, Part of Bio-Techne

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class="icon-icon-product-citations d-none d-lg-inline pr-1"></span> Citations (363)  </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <a href="#product-documents" class="datasheet_link product-documents" rel="nofollow">Product Datasheet / COA / SDS</a> </div> </div> </div> </div> <div id="new_version_available"> <a class="font-weight-bold" href="/p/elisa-kits/substrate-reagent-pack_dy999b"> New Version Available - DY999B </a> </div> <div class="row mb-2" id="extended-description"> <div class="col-md-10 extended-description-wrapper" id="extended-description-wrapper"> 8 vials Color A, 8 vials Color B </div> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> <div id="product_alerts_wrapper"><article class="product-data-wrapper"> </article> </div> <div class="datapoint_list" id="key_details"><h3>Key Product Details</h3></div> </div> <div class="row border-top mb-2" id="images_atc_orderinfo_wrapper"><div class="col-xl-9 mt-2" id="image_atc_keydetails_wrapper"><div class="row" id="image_atc_wrapper"><div class="col-xl-4 order-xl-first" id="image_wrapper"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.bio-techne.com/images/products/elisa/General_Laboratory_Research_Reagent_DY999_ELISA_2587.jpg" alt="Substrate Reagent Pack" title="Substrate Reagent Pack" data-caption="" data-badges="" data-application="N/A" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </article> </div> <div class="col-xl-8" id="atc_wrapper"><article class="product-data-wrapper">  <div class="atc-container"> <div class="commerce-atc-datasheet atc-block" data-type="ds" data-blockid="ProductCart"> <form action="/commerce/addtocart" method="post" class="add-to-cart-block" data-productcode="DY999"> <input type="hidden" name="print-quote" class="print-quote-input" value=""> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-3 col-md-3 font-weight-bold p-0">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-4 font-weight-bold d-none d-md-block">Availability</div> <div class="commerce-atc-price atc-head col-4 col-md-4 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-4 col-3 col-md-1 text-right font-weight-bold p-0">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart" class="atc-lines"> <div class="commerce-atc-ds-productline productline col-four row my-2 mx-0 text-left" data-catalog="DY999" data-hidden=""> <div class="commerce-atc-catnum col-3 col-md-3 pl-0 m-0">DY999</div> <div class="commerce-atc-availability col-md-4 pl-0 m-0 d-none d-md-block"> </div> <div class="commerce-atc-price col-4 col-md-3 pl-0 m-0"> </div> <div class="commerce-atc-quantity col-4 col-md-2 text-right px-0 m-0"> </div> </div> </div> <section id="section-ProductCart-ancillary" class="d-none"> <hr> <span class="section-title col border-bottom pb-1 d-block pl-0">Ancillary Products Available</span> <div id="ProductCart-ancillary" class="atc-ancillary"> <div class="commerce-atc-ds-productline-ancillary ancillary-productline col-four row mb-5" data-catalog="DY008B" data-hidden=""> <div class="commerce-atc-title col-md-5 p-0"> <a href=""></a> </div> <div class="commerce-atc-price col-md-2 p-0 text-right"> </div> <div class="commerce-atc-quantity col-md-5 text-right"> </div> </div> </div> </section> <div class="atc-control ProductCart-control text-right row mx-0"> <a href="" class="atc-print-quote-button btn btn-secondary m-1 col-xs-4">Print Quote</a> <a href="/services/bulk-quotes?product_code=DY999" class="atc-bulk-button btn btn-secondary m-1 col-xs-4" rel="nofollow">Bulk Order</a> <input type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-0 col-xs-4"/> </div> </div> </form> </div> </div> </article> </div> </div> <div class="row datapoint_list border-top" id="key_details_wrapper"><div class="col-xl-12 mt-2" id="key_details_col_wrapper"></div> </div> </div> <div class="col-xl-3 mt-2" id="orderinfo_guarantee_wrapper"><div id="order_information_wrapper"><div id="product_shipping_block_wrapper" data-brand="rnd" data-shipping="" data-source="ep-data" data-sub_category_id="50"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"><span class="sr-only">Loading...</span></div> </div> </div> <div id="video-tile-wrapper"><article class="product-data-wrapper"> <div class="video_tile_wrapper"> <article> <div> <lite-youtube videoid="9QE6w3gImsM" style="background-image: url('https://img.youtube.com/vi/9QE6w3gImsM/maxresdefault.jpg');"> <a href="https://www.youtube.com/embed/9QE6w3gImsM?autoplay=1" class="lty-playbtn" title="Setting the standard in quality research reagents"> <span class="lyt-visually-hidden">Setting the standard in quality research reagents</span> </a> </lite-youtube> </div> </article> </div> </article> </div> <div id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> </div> <div class="tab-content rounded-bottom border-0 mb-3 shadow"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="technical-data-tab-tab" href="#technical-data-tab" data-toggle="tab" role="tab" aria-controls="region-4821584720927" aria-selected="true" >Technical Data</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-reviews--tab-tab" href="#citations-reviews--tab" data-toggle="tab" role="tab" aria-controls="region-7631584720992" aria-selected="false" >Citations / Reviews (51)</a> </li> </ul> <div class="tab-pane fade mx-4 py-4 show" id="procedure-tab" data-tab-label="Procedure" role="tabpanel" aria-labelledby="procedure-tab-tab"></div> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4 cell-culture-gpp" id="technical-data-tab" data-tab-label="Technical Data" role="tabpanel" aria-labelledby="technical-data-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="product-summary_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#product-summary" id="product-summary_li" >Product Summary</a> </li> <li id="product-specifications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#product-specifications" id="product-specifications_li" >Product Specifications</a> </li> <li id="preparation--storage_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#preparation--storage" id="preparation--storage_li" >Preparation & Storage</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="product-summary" class="nav-link px-0" data-anchor-label="Product Summary"><div id="product_title_summary"><h3>Summary for Substrate Reagent Pack</h3><article class="product-data-wrapper"> <html><body><p>INTENDED USE & DESCRIPTION</p> <p>Substrate Reagent Pack contains 8 vials of Color Reagent A (stabilized hydrogen peroxide, Part # 895000) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine, Part # 895001), both at 12 mL/vial, producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies.   </p> <p>STORAGE & STABILITY</p> <p>Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.</p> <p>REAGENT PREPARATION</p> <p>Warm to room temperature before use. Color Reagent A and Color Reagent B should be mixed together in equal volumes within 15 minutes of use. Protect from light. Add 100 µL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.</p> <p>TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE </p> <ul> <li>Caution: Color Reagent B may cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use. </li> </ul></body></html> </article> </div> </div> <div id="product-specifications" class="nav-link px-0" data-anchor-label="Product Specifications"><table class="tablesaw tablesaw-stack table-bordered table" id="product_specification_wrapper"><h3>Product Specifications for Substrate Reagent Pack</h3><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Applications</td> <td class="product-data-value p-3 px-4 text-break"> ELISA </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Source</td> <td class="product-data-value p-3 px-4 text-break"> N/A </td> </tr> </table> </div> <div id="preparation--storage" class="nav-link px-0" data-anchor-label="Preparation & Storage"><div id="cc_prep_storage"><h3>Preparation & Storage</h3><table class="table-bordered table" id="prep_storage_wrapper"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Shipping Conditions</td> <td class="product-data-value p-3 px-4 text-break"> The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store the unopened product at 2 - 8 °C. Do not use past expiration date. </td> </tr> </table> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4 show" id="citations-reviews--tab" data-tab-label="Citations / Reviews (51)" role="tabpanel" aria-labelledby="citations-reviews--tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#reviews"> Reviews </a> </li> </div> </div> <div id="citations"> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="DY999"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> </section> </div> <div 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</div> <section class="row"> <div class="col-md-6"> <form novalidate="novalidate" data-drupal-selector="product-coa-form"> <div class="form-row align-items-center"> <div class="col-md-10 my-1"> <input data-drupal-selector="form-rfmdc-y6z2odzwtmbkvhbun7sfnc5cfd58r4xqkf-ue" type="hidden" name="form_build_id" value="form-rFMDC_Y6z2OdzWtMBKvhbuN7sfNc5cFd58R4XqKF_UE" /> <input data-drupal-selector="edit-product-coa-form" type="hidden" name="form_id" value="product_coa_form" /> <input data-drupal-selector="edit-product-code" type="hidden" name="product_code" value="DY999" /> <div class="js-form-item form-item pb-3 js-form-type-textfield form-item-lot-number js-form-item-lot-number form-no-label"> <input autocomplete="off" class="form-control cofa-lookup form-text" id="lot-number" placeholder="Search by lot number" data-drupal-selector="edit-lot-number" type="text" name="lot_number" value="" size="60" maxlength="128" /> </div> <input data-drupal-selector="edit-brand" type="hidden" 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Substrate Reagent Pack (DY999) by R&D Systems, Part of Bio-Techne