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PBMC Isolation · 360biolabs
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The accuracy and reliability of data generated from assays that utilise PBMCs hinge on the prompt collection, skilled isolation, handling, and storage of samples.</p> </div> </div> </div> <img src="https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/hero-shape.svg" alt="Decorative image" class="bottom-decoration" data-neruda-anim="hero-shape"> <div class="icon-bg" style="background-image:url('https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/icons-library/icon-pbmc-default.svg')" data-neruda-anim="fade-left"></div> </section> <div class="services-page-anchor"> <div class="button-page-scroll-container"> <a href="#page-content" class="button-page-scroll"> <span aria-hidden="false">Scroll to page content</span> </a> </div> <a id="page-content"></a> </div> <section class="content-block block-pill-image-text"> <div class="container is-wide"> <div class="columns"> <div class="column-content"> <div class="inner-padding"> <h2>Expertise With<br /> PBMC Isolation:</h2> <div class="features-container"> <div class="feature" style="background-image:url('https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/icons-library/icon-measuring-cylinder-circle.svg');"> <h3>Experienced in Various Isolation Techniques</h3> <p>Our expertise includes transitional density gradient underlay, SepMate™, LeucoSep™, and CPT™. Our extensive SOP list has over 50 custom methods.</p> </div> <div class="feature" style="background-image:url('https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/icons-library/icon-quality-circle.svg');"> <h3>Quality Assurance</h3> <p>Accreditation by Australia’s National Association of Testing Authorities (NATA) and participation in external and internal proficiency programs ensures consistent, high-quality isolation of PBMCs.</p> </div> <div class="feature" style="background-image:url('https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/icons-library/icon-lab-flask-circle.svg');"> <h3>Flexibility</h3> <p>Our onsite phlebotomists ensure we can provide donor whole blood for study-specific proficiency testing, and our comprehensive immunology profiling services allow for downstream assay support or shipment of PBMCs to a client’s selected laboratory.</p> </div> </div> </div> </div> <div class="column-image"> <div class="image-container" > <figure> <img width="1920" height="1280" src="https://www.360biolabs.com/wp-content/uploads/PBMC_6434.webp" class="attachment-full size-full" alt="Scientist isolating PBMCs from whole blood via traditional ficoll underlay" decoding="async" fetchpriority="high" srcset="https://www.360biolabs.com/wp-content/uploads/PBMC_6434.webp 1920w, https://www.360biolabs.com/wp-content/uploads/PBMC_6434-600x400.webp 600w, https://www.360biolabs.com/wp-content/uploads/PBMC_6434-1400x933.webp 1400w, https://www.360biolabs.com/wp-content/uploads/PBMC_6434-768x512.webp 768w, https://www.360biolabs.com/wp-content/uploads/PBMC_6434-1536x1024.webp 1536w" sizes="(max-width: 1920px) 100vw, 1920px" /> </figure> <div class="shape has-cyan-fair-bg" ></div> </div> <div class="section-action"> <a href="https://www.360biolabs.com/contact/" target="_self"> Choose the right Central Lab service with our help</a> </div> </div> </div> </div> </section> <section class="content-block block-modalities-assays is-pbmc has-steel-fair-bg"> <div class="container"> <h2>360biolabs Central Lab Services</h2> <div class="intro-text"> <p>As part of 360biolabs Central Laboratory Services, we offer PBMC isolation and RNA/DNA, with the added capability to handle more complex matrices and coordinate global shipping to any preferred laboratory. </p> </div> <div class="services-container"> <div class="service-card" style="background-image:url('https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/icons-library/icon-cell-single-default.svg')"> <h3>PBMC Isolation</h3> <ul> <li>Study specific proficiency testing</li> <li>Temperature monitored shipments</li> <li>Onsite 24/7 temperature monitoring</li> <li>GLP, GCP, ISO17025 accredited laboratories</li> <li>Time sensitive isolation via our multiple sites</li> <li>Experienced in isolating PBMCs from: <ul> <li>ACD</li> <li>EDTA</li> <li>Sodium Heparin</li> <li>Lithium Heparin</li> <li>CPT</li> </ul> </li> </ul> </div> <div class="service-card" style="background-image:url('https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/icons-library/icon-molecule-default.svg')"> <h3>RNA/DNA Extraction</h3> <ul> <li>Extraction of high-quality RNA and DNA</li> <li>Small scale extraction using Spin Column technology: Mini, Midi & Maxi</li> <li>Automated extraction process: Magnetic Bead based extraction system</li> <li>Matrices we support: <ul> <li> Whole blood</li> <li>Serum (Circulating Tumor DNA)</li> <li>Plasma</li> <li>Stool</li> <li>Urine</li> <li> Oral mucosall swab</li> <li>Injection site swab</li> <li> Biopsies (Fresh Frozen or FFPE fixed)</li> <li>Cell Culture supernatant</li> <li>Nasal mucosal lining fluid</li> <li>Saliva</li> <li>Synovial fluid</li> <li>Whole blood</li> <li>CSF</li> <li>Wound fluid</li> </ul> </li> </ul> </div> <div class="service-card" style="background-image:url('https://www.360biolabs.com/wp-content/themes/cc-360biolabs/assets/images/icons-library/icon-vaccines-default.svg')"> <h3>Specialised Matrix</h3> <ul> <li>Processing of specialised matrices: <ul> <li>Bone marrow aspirate</li> <li>Sputum</li> </ul> </li> </ul> </div> </div> <div class="section-actions"> <a href="https://www.360biolabs.com/contact/" target="_self" class="button"> <span>Discuss Clinical Study</span> </a> <a href="https://www.360biolabs.com/australian-locations/" target="_self" class="button is-outlined"> <span>Locations</span> </a> </div> </div> <div class="hero-image content-block block-hero-image has-half-bg"> <div class="container is-default"> <figure> <img width="1920" height="1280" src="https://www.360biolabs.com/wp-content/uploads/PBMC_6484.webp" class="attachment-full size-full" alt="Scientist isolating the Peripheral Blood Mononuclear Cells (PBMC) layer between plasma and ficoll density gradient medium" decoding="async" srcset="https://www.360biolabs.com/wp-content/uploads/PBMC_6484.webp 1920w, https://www.360biolabs.com/wp-content/uploads/PBMC_6484-600x400.webp 600w, https://www.360biolabs.com/wp-content/uploads/PBMC_6484-1400x933.webp 1400w, https://www.360biolabs.com/wp-content/uploads/PBMC_6484-768x512.webp 768w, https://www.360biolabs.com/wp-content/uploads/PBMC_6484-1536x1024.webp 1536w" sizes="(max-width: 1920px) 100vw, 1920px" /> </figure> </div> </div> </section> <section class="content-block block-related-content"> <div class="container"> <h2 class="is-centered">Related Content</h2> <div class="columns"> <div class="column"> <figure> <img width="600" height="400" src="https://www.360biolabs.com/wp-content/uploads/Bri_Lab_6741-600x400.webp" class="attachment-medium size-medium" alt="Team of scientists working in a bioanalytical laboratory" decoding="async" srcset="https://www.360biolabs.com/wp-content/uploads/Bri_Lab_6741-600x400.webp 600w, https://www.360biolabs.com/wp-content/uploads/Bri_Lab_6741-1400x933.webp 1400w, https://www.360biolabs.com/wp-content/uploads/Bri_Lab_6741-768x512.webp 768w, https://www.360biolabs.com/wp-content/uploads/Bri_Lab_6741-1536x1024.webp 1536w, https://www.360biolabs.com/wp-content/uploads/Bri_Lab_6741.webp 1920w" sizes="(max-width: 600px) 100vw, 600px" /> </figure> <div class="text-container"> <h3>ELISpot</h3> <div class="column-blurb"> <p>ELISpot measures the frequency of antigen-specific cells by measuring the number of cytokine-secreting cells in PBMC at a single cell level.</p> </div> <p class="column-action"> <a href="https://www.360biolabs.com/contact/"> Learn more</a> </p> </div> </div> <div class="column"> <figure> <img width="600" height="400" src="https://www.360biolabs.com/wp-content/uploads/Flow_6839-600x400.webp" class="attachment-medium size-medium" alt="Scientist measuring cell populations in whole blood via our Fortessa flow cytometer" decoding="async" loading="lazy" srcset="https://www.360biolabs.com/wp-content/uploads/Flow_6839-600x400.webp 600w, https://www.360biolabs.com/wp-content/uploads/Flow_6839-1400x933.webp 1400w, https://www.360biolabs.com/wp-content/uploads/Flow_6839-768x512.webp 768w, https://www.360biolabs.com/wp-content/uploads/Flow_6839-1536x1024.webp 1536w, https://www.360biolabs.com/wp-content/uploads/Flow_6839.webp 1920w" sizes="(max-width: 600px) 100vw, 600px" /> </figure> <div class="text-container"> <h3>Flow Cytometry</h3> <div class="column-blurb"> <p>Using high-parameter single-cell analysis, flow cytometry can identify granular changes in response to a therapeutic.</p> </div> <p class="column-action"> <a href="https://www.360biolabs.com/flow-cytometry/"> Learn more</a> </p> </div> </div> <div class="column"> <figure> <img width="600" height="400" src="https://www.360biolabs.com/wp-content/uploads/PCR_MCP_6884-1-600x400.webp" class="attachment-medium size-medium" alt="High throughput extraction of RNA via Kingfisher automated extractor prior to amplification via polymerase chain reaction (PCR) on a ThermoFisher Quant Studio 7" decoding="async" loading="lazy" srcset="https://www.360biolabs.com/wp-content/uploads/PCR_MCP_6884-1-600x400.webp 600w, https://www.360biolabs.com/wp-content/uploads/PCR_MCP_6884-1-1400x933.webp 1400w, https://www.360biolabs.com/wp-content/uploads/PCR_MCP_6884-1-768x512.webp 768w, https://www.360biolabs.com/wp-content/uploads/PCR_MCP_6884-1-1536x1024.webp 1536w, https://www.360biolabs.com/wp-content/uploads/PCR_MCP_6884-1.webp 1772w" sizes="(max-width: 600px) 100vw, 600px" /> </figure> <div class="text-container"> <h3>Molecular Assays</h3> <div class="column-blurb"> <p>Using high-parameter single-cell analysis, flow cytometry can identify granular changes in response to a therapeutic.</p> </div> <p class="column-action"> <a href="https://www.360biolabs.com/molecular-assays/"> Learn more</a> </p> </div> </div> </div> </div> </section> </main> <section class="block-footer-cta"> <div class="container is-default"> <div class="columns"> <div class="column-text"> <p>Enabling future medicine together. 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