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accession</title> <meta name="description" content="Search results for: accession"> <meta name="keywords" content="accession"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="accession"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 78</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: accession</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">78</span> Good Faith and Accession in the New Civil Code</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adelina%20Vrancianu">Adelina Vrancianu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The problem of artificial real accession will be analyzed in this study both in terms of old and current Civil Code provisions and in terms of comparative law, European legal and Canadian systems. The current Civil Code from 2009 has brought new changes about the application and solutions regarding artificial real accession. The hypothesis in which a person is making works with his own materials on the real estate belonging to another person is developed and analyzed in detail from national and international point of view in relation with the good faith. The scope of this analysis is to point out what are the changes issued from case-law and which ones are new, inspired from other law systems in regard to the good/bad faith. The new civil code has promoted a definition for this notion. Is this definition a new one inspired from the comparative law or is it inspired from the case-law? Is it explained for every case scenario of accession or is a general notion? The study tries to respond to these questions and to present the new aspects in the area. has reserved a special place for the situation of execution of works with own materials exceeding the border with violation of another’s right of property, where the variety of solutions brings into discussion the case of expropriation for private interest. The new Civil Code is greatly influenced by the Civil Code from Quebec in comparison with the old code of French influence. The civil reform was needed and has brought into attention new solutions inspired from the Canadian system which has mitigated the permanent conflict between the constructor and the immovable owner. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=accession" title="accession">accession</a>, <a href="https://publications.waset.org/abstracts/search?q=good%20faith" title=" good faith"> good faith</a>, <a href="https://publications.waset.org/abstracts/search?q=new%20civil%20code" title=" new civil code"> new civil code</a>, <a href="https://publications.waset.org/abstracts/search?q=comparative%20law" title=" comparative law "> comparative law </a> </p> <a href="https://publications.waset.org/abstracts/23497/good-faith-and-accession-in-the-new-civil-code" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23497.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">462</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">77</span> Benefits of Polish Accession to the European Union for Air Transport</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20Tloczynski">D. Tloczynski</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The main aim of this article is to present a balance of the decade of Polish air transport market in the European Union having taking into account selected entities of the aviation market. This article analyzes the functioning of the Polish air transport market after the Polish accession to the European Union. During the study two main areas were pointed: shipping activity and activity of the airports. The most important benefits of integration and the benefits of introducing of the open sky policy were indicated. The last part of the article presents the perspectives of development of air traffic. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=air%20transport" title="air transport">air transport</a>, <a href="https://publications.waset.org/abstracts/search?q=airports" title=" airports"> airports</a>, <a href="https://publications.waset.org/abstracts/search?q=development%20air%20transport" title=" development air transport"> development air transport</a>, <a href="https://publications.waset.org/abstracts/search?q=European%20Union" title=" European Union"> European Union</a>, <a href="https://publications.waset.org/abstracts/search?q=Poland" title=" Poland"> Poland</a> </p> <a href="https://publications.waset.org/abstracts/16397/benefits-of-polish-accession-to-the-european-union-for-air-transport" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16397.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">442</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">76</span> Effectiveness of ISSR Technique in Revealing Genetic Diversity of Phaseolus vulgaris L. Representing Various Parts of the World</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20El-Shikh">Mohamed El-Shikh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Phaseolus vulgaris L. is the world’s second most important bean after soybeans; used for human food and animal feed. It has generally been linked to reduced risk of cardiovascular disease, diabetes mellitus, obesity, cancer and diseases of digestive tract. The effectiveness of ISSR in achievement of the genetic diversity among 60 common bean accessions; represent various germplasms around the world was investigated. In general, the studied Phaseolus vulgaris accessions were divided into 2 major groups. All of the South-American accessions were separated into the second major group. These accessions may have different genetic features that are distinct from the rest of the accessions clustered in the major group. Asia and Europe accessions (1-20) seem to be more genetically similar (99%) to each other as they clustered in the same sub-group. The American and African varieties showed similarities as well and clustered in the same sub-tree group. In contrast, Asian and American accessions No. 22 and 23 showed a high level of genetic similarities, although these were isolated from different regions. The phylogenetic tree showed that all the Asian accessions (along with Australian No. 59 and 60) were similar except Indian and Yemen accessions No. 9 and 20. Only Netherlands accession No. 3 was different from the rest of European accessions. Morocco accession No. 52 was genetically different from the rest of the African accessions. Canadian accession No. 44 seems to be different from the other North American accessions including Guatemala, Mexico and USA. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phylogenetic%20tree" title="phylogenetic tree">phylogenetic tree</a>, <a href="https://publications.waset.org/abstracts/search?q=Phaseolus%20vulgaris" title=" Phaseolus vulgaris"> Phaseolus vulgaris</a>, <a href="https://publications.waset.org/abstracts/search?q=ISSR%20technique" title=" ISSR technique"> ISSR technique</a>, <a href="https://publications.waset.org/abstracts/search?q=genetics" title=" genetics"> genetics</a> </p> <a href="https://publications.waset.org/abstracts/27152/effectiveness-of-issr-technique-in-revealing-genetic-diversity-of-phaseolus-vulgaris-l-representing-various-parts-of-the-world" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27152.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">408</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">75</span> Screening Some Accessions of Lentil (Lens culinaris M.) for Salt Tolerance at Germination and Early Seedling Stage in Eastern Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Azene%20Tesfaye">Azene Tesfaye</a>, <a href="https://publications.waset.org/abstracts/search?q=Yohannes%20Petros"> Yohannes Petros</a>, <a href="https://publications.waset.org/abstracts/search?q=Habtamu%20Zeleke"> Habtamu Zeleke </a> </p> <p class="card-text"><strong>Abstract:</strong></p> To evaluate genetic variation among Ethiopian lentil, laboratory experiment were conducted to screen 12 accessions of lentil (Lens culinaris M.) for salt tolerance. Seeds of 12 Lentil accessions were grown at laboratory (Petri dish) condition with different levels of salinity (0, 2, 4, and 8 dSm-1 NaCl) for 4 weeks. The experimental design was completely randomized design (CRD) in factorial combination with three replications. Data analysis was carried out using SAS software. Average germination time, germination percentage, seedling shoot and root traits, seedling shoot and root weight were evaluated. The two way ANOVA for varieties revealed statistically significant variation among lentil accession, NaCl level and their interactions (p<0.001) with respect to the entire parameters. It was found that salt stress significantly delays germination rate and decreases germination percentage, shoot and root length, seedling shoot and root weight of lentil accessions. The degree of decrement varied with accessions and salinity levels. Accessions 36120, 9235 and 36004 were better salt tolerant than the other accessions. As the result, it is recommended to be used as a genetic resource for the development of lentil accession and other very salt sensitive crop with improved germination under salt stress condition. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=accession" title="accession">accession</a>, <a href="https://publications.waset.org/abstracts/search?q=germination" title=" germination"> germination</a>, <a href="https://publications.waset.org/abstracts/search?q=lentil" title=" lentil"> lentil</a>, <a href="https://publications.waset.org/abstracts/search?q=NaCl" title=" NaCl"> NaCl</a>, <a href="https://publications.waset.org/abstracts/search?q=screening" title=" screening"> screening</a>, <a href="https://publications.waset.org/abstracts/search?q=seedling%20stage" title=" seedling stage"> seedling stage</a> </p> <a href="https://publications.waset.org/abstracts/29040/screening-some-accessions-of-lentil-lens-culinaris-m-for-salt-tolerance-at-germination-and-early-seedling-stage-in-eastern-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29040.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">340</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">74</span> Evaluation of Phytochemical and Fatty Acids Content and Composition in Iranian Borage (Echium amoenum) in Different Habitate of Iran</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Esmaeil%20Babakhanzadeh%20Sajirani">Esmaeil Babakhanzadeh Sajirani</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamadjavad%20Shakouri"> Mohamadjavad Shakouri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Iranian Gole GavZaban (Echium amoenum fich & mey), is one of the most important medicinal plant in north of iran . is dry petals used for tonic, tranquillizer, diaphoretic, cough suppressant and a remedy for sore throat in treditional Iranian medicine. This study is the report about the analysis of phytochemical and seeds oil of Echium amoenum's in different habitates and accessions of Iran. The results showed that the oil content of seeds was 36% and eleven fatty acids were identified and quantified by gas chromatography (GC). The major fatty acids wereα-Linolenicacid (39.99), Linoleic acid (20.86), linolenic acid (20%) and Oleic acid (15.36) respectively. The amount of phenols, tannins, flavonoids and anthocyanins with increasing height, increased amount of these compounds. So that the highest rates of these compounds were observed at an altitude of 2125 meters in ciposht accession. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=accession" title="accession">accession</a>, <a href="https://publications.waset.org/abstracts/search?q=phytochemical" title=" phytochemical"> phytochemical</a>, <a href="https://publications.waset.org/abstracts/search?q=oil%20components" title=" oil components"> oil components</a>, <a href="https://publications.waset.org/abstracts/search?q=Iranian%20borage" title=" Iranian borage"> Iranian borage</a> </p> <a href="https://publications.waset.org/abstracts/59008/evaluation-of-phytochemical-and-fatty-acids-content-and-composition-in-iranian-borage-echium-amoenum-in-different-habitate-of-iran" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59008.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">252</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">73</span> Sentiment Analysis on the East Timor Accession Process to the ASEAN</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marcelino%20Caetano%20Noronha">Marcelino Caetano Noronha</a>, <a href="https://publications.waset.org/abstracts/search?q=Vosco%20Pereira"> Vosco Pereira</a>, <a href="https://publications.waset.org/abstracts/search?q=Jose%20Soares%20Pinto"> Jose Soares Pinto</a>, <a href="https://publications.waset.org/abstracts/search?q=Ferdinando%20Da%20C.%20Saores"> Ferdinando Da C. Saores</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One particularly popular social media platform is Youtube. It’s a video-sharing platform where users can submit videos, and other users can like, dislike or comment on the videos. In this study, we conduct a binary classification task on YouTube’s video comments and review from the users regarding the accession process of Timor Leste to become the eleventh member of the Association of South East Asian Nations (ASEAN). We scrape the data directly from the public YouTube video and apply several pre-processing and weighting techniques. Before conducting the classification, we categorized the data into two classes, namely positive and negative. In the classification part, we apply Support Vector Machine (SVM) algorithm. By comparing with Naïve Bayes Algorithm, the experiment showed SVM achieved 84.1% of Accuracy, 94.5% of Precision, and Recall 73.8% simultaneously. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=classification" title="classification">classification</a>, <a href="https://publications.waset.org/abstracts/search?q=YouTube" title=" YouTube"> YouTube</a>, <a href="https://publications.waset.org/abstracts/search?q=sentiment%20analysis" title=" sentiment analysis"> sentiment analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=support%20sector%20machine" title=" support sector machine"> support sector machine</a> </p> <a href="https://publications.waset.org/abstracts/162327/sentiment-analysis-on-the-east-timor-accession-process-to-the-asean" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162327.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">108</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">72</span> Identification and Characterization of 18S rRNA Gene of Demodex Canis From the Dog Population of Mizoram, India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Moneesh%20Thakur">Moneesh Thakur</a>, <a href="https://publications.waset.org/abstracts/search?q=Hridayesh%20Prasad"> Hridayesh Prasad</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikitasha%20Bora"> Nikitasha Bora</a>, <a href="https://publications.waset.org/abstracts/search?q=Parimal%20Roy%20Choudhary"> Parimal Roy Choudhary</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20Samanta"> A. K. Samanta</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanjeev%20Kumar"> Sanjeev Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Canine demodicosis is a common parasitic condition which involves dog skin. Demodicosis in dogs is due the prominent growth of Demodex. Out of various canine Demodex spp., Demodex canis is the most often involved species. Canine demodicosis can occur as either a localized or generalized form of demodicosis severely affect the dogs and in non-treated dogs may cause death. This study was planned with the aim to screen and characterize the 18S rRNA gene of isolated Demodex canis. A total of 1200 dogs were screened during this study period. The skin scrapings of all the suspected dogs were examined under a microscope at 100X magnification for the presence of Demodex canis. The skin scrapings positive for Demodex canis were examined using PCR for confirmation. A total of 35 dogs were confirmed a positive result for D. canis based on 18S rRNA gene amplification by PCR. Further, the 18S rRNA gene of isolated Demodex canis was cloned and sequenced for genome analysis. On the sequence analysis, it was found that isolated sequence (GenBank Accession No. MK177513) had close similarity (99.7%) to that of D. canis genotype of China (Accession No. MG372254). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=PCR" title="PCR">PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=phylogenetic%20analysis" title=" phylogenetic analysis"> phylogenetic analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=cloning%20and%20sequening" title=" cloning and sequening"> cloning and sequening</a>, <a href="https://publications.waset.org/abstracts/search?q=Demodex%20canis" title=" Demodex canis"> Demodex canis</a> </p> <a href="https://publications.waset.org/abstracts/172036/identification-and-characterization-of-18s-rrna-gene-of-demodex-canis-from-the-dog-population-of-mizoram-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/172036.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">93</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">71</span> Yield, Biochemical Responses and Evaluation of Drought Tolerance of Two Barley Accessions 'Ardhaoui' under Deficit Drip Irrigation Using Saline Water in Southern Tunisia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Bagues">Mohamed Bagues</a>, <a href="https://publications.waset.org/abstracts/search?q=Ikbel%20Souli"> Ikbel Souli</a>, <a href="https://publications.waset.org/abstracts/search?q=Feiza%20Boussora"> Feiza Boussora</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamel%20Nagaz"> Kamel Nagaz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In southern Tunisia, two local barley accessions CV. Ardhaoui; 'Bengardeni' and 'Karkeni' were cultivated in the field under deficit drip irrigation with saline water. Three treatments were used: control or full irrigation T0 (100%ETc) and stressed T1 (75%ETc), T2 (50%ETc). Proline and soluble sugars contents increase significantly under drought between accessions compared to control and varies between growth stages. Moreover, the increasing of Ca2+ concentration enhances the absorption of Na+ ion, consequently K+/Na+ decrease significantly between accessions, these results suggest that a high tolerance of Bengardeni accession to drought stress. Therefore, drought tolerance indices (STI, SSI, MP, GMP, YSI and TOL) were used to identify high yielding and drought tolerant between accessions. MP explained the variation of GYi. GMP and STI explained the variation of GYs. The high values of MP, STI and GMP were associated with higher yielding accession. Higher TOL value is associated with significant grain yield reduction in stressed environment suggesting higher stress responses of accessions. Significant positive correlations between MP, STI and GMP and negative between YSI and SSI. MP, STI, GMP and YSI, TOL, SSI are not correlated with each other. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drought" title="drought">drought</a>, <a href="https://publications.waset.org/abstracts/search?q=proline" title=" proline"> proline</a>, <a href="https://publications.waset.org/abstracts/search?q=soluble%20sugars" title=" soluble sugars"> soluble sugars</a>, <a href="https://publications.waset.org/abstracts/search?q=minerals" title=" minerals"> minerals</a>, <a href="https://publications.waset.org/abstracts/search?q=yield" title=" yield"> yield</a>, <a href="https://publications.waset.org/abstracts/search?q=drought%20tolerance%20indices" title=" drought tolerance indices"> drought tolerance indices</a>, <a href="https://publications.waset.org/abstracts/search?q=barley" title=" barley"> barley</a> </p> <a href="https://publications.waset.org/abstracts/53059/yield-biochemical-responses-and-evaluation-of-drought-tolerance-of-two-barley-accessions-ardhaoui-under-deficit-drip-irrigation-using-saline-water-in-southern-tunisia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53059.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">242</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">70</span> Comparative Assessment of the Potential Impact of Joining the World Trade Organization and African Continental Free Trade Area on the Ethiopia Economy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Agidew%20Abay">Agidew Abay</a>, <a href="https://publications.waset.org/abstracts/search?q=Nobuhiro%20Hosoe"> Nobuhiro Hosoe</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ethiopia signed the AfCFTA in 2018 and is in ongoing negotiations to join the WTO. To assess the potential impacts of joining these trade agreements on Ethiopia's trade, output, and welfare, we conducted a comprehensive analysis using a world trade computable general equilibrium (CGE) model. The results of our policy experiment, which include scenarios involving the reduction of tariff and non-tariff measures, indicate that AfCFTA and WTO accession would positively affect Ethiopia's welfare, with WTO membership expected to bring more significant benefits. On the one hand, AfCFTA membership would significantly increase Ethiopian imports from AfCFTA regions while decreasing imports from non-AfCFTA regions. Conversely, it would boost Ethiopian exports to Southern Africa while showing minimal change to other AfCFTA and non-AfCFTA regions. By contrast, WTO membership would significantly increase Ethiopia’s imports from Asia and North Africa and decrease those from Europe, the rest of the world, and East Africa. It would increase exports to all regions, especially Europe, Asia, and the rest of the world. In terms of industrial output, while these two trade deals would largely favor agriculture and the meat and livestock sector and harm many manufacturing sectors (especially the light manufacturing sector), the impact of WTO accession on the Ethiopian economy would be overwhelmingly more significant than that of AfCFTA. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=trade%20liberalization" title="trade liberalization">trade liberalization</a>, <a href="https://publications.waset.org/abstracts/search?q=AfCFTA" title=" AfCFTA"> AfCFTA</a>, <a href="https://publications.waset.org/abstracts/search?q=WTO" title=" WTO"> WTO</a>, <a href="https://publications.waset.org/abstracts/search?q=computable%20general%20equilibrium%20model" title=" computable general equilibrium model"> computable general equilibrium model</a>, <a href="https://publications.waset.org/abstracts/search?q=tariff" title=" tariff"> tariff</a>, <a href="https://publications.waset.org/abstracts/search?q=non-tariff%20measures" title=" non-tariff measures"> non-tariff measures</a> </p> <a href="https://publications.waset.org/abstracts/194418/comparative-assessment-of-the-potential-impact-of-joining-the-world-trade-organization-and-african-continental-free-trade-area-on-the-ethiopia-economy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/194418.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">8</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">69</span> Genotypic Response Differences among Faba Bean Accessions under Regular Deficit Irrigation (RDI)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Afzal">M. Afzal</a>, <a href="https://publications.waset.org/abstracts/search?q=Salem%20Safer%20Alghamdi"> Salem Safer Alghamdi</a>, <a href="https://publications.waset.org/abstracts/search?q=Awais%20Ahmad"> Awais Ahmad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Limited amount of irrigation water is an alarming threat to arid and semiarid agriculture. However, genotypic response differences to water deficit conditions within species have been reported frequently. Present study was conducted in order to measure the genotypic differences among faba bean accessions under Regular Deficit Irrigation (RDI). Five seeds from each accession were sown in 135 silt filled pots (30 x 24 cm). Experiment was planned under split plot arrangement and replicated thrice. Treatments consisted of three RDI levels (100% (control), 60% and 40% of the field capacity) and fifteen faba bean accessions (two local accessions as reference while thirteen from different sources around the world). Irrigation treatment was started from the very first day of sowing. Plant height, shoot dry weight, stomatal conductance and total chlorophyll contents (SPAD reading) were measured one month after germination. Irrigation, faba bean accessions and the all possible interactions has stood significantly high for all studied parameters. Regular deficient irrigation has hampered the plant growth and associated parameters in decreasing order (100% < 60% < 40%). Accessions have responded differently under regular deficient irrigation and some of them are even better than local accession. A highly significant correlation among all parameters has also been observed. It was concluded from results that above parameters could be used as markers to identify the genotypic differences for water deficit stress response. This outcome encouraged the use of superior faba bean genotypes in breeding programs for improved varieties to enhance water use efficiency under stress conditions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=accessions" title="accessions">accessions</a>, <a href="https://publications.waset.org/abstracts/search?q=stomatal%20conductance" title=" stomatal conductance"> stomatal conductance</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20chlorophyll%20contents" title=" total chlorophyll contents"> total chlorophyll contents</a>, <a href="https://publications.waset.org/abstracts/search?q=RDI" title=" RDI"> RDI</a>, <a href="https://publications.waset.org/abstracts/search?q=regular%20deficient%20irrigation" title=" regular deficient irrigation"> regular deficient irrigation</a> </p> <a href="https://publications.waset.org/abstracts/5157/genotypic-response-differences-among-faba-bean-accessions-under-regular-deficit-irrigation-rdi" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/5157.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">297</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">68</span> Isolation and Characterization of Cotton Infecting Begomoviruses in Alternate Hosts from Cotton Growing Regions of Pakistan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Irfan%20Fareed">M. Irfan Fareed</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Tahir"> Muhammad Tahir</a>, <a href="https://publications.waset.org/abstracts/search?q=Alvina%20Gul%20Kazi"> Alvina Gul Kazi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Castor bean (Ricinus communis; family Euphorbiaceae) is cultivated for the production of oil and as an ornamental plant throughout tropical regions. Leaf samples from castor bean plants with leaf curl and vein thickening were collected from areas around Okara (Pakistan) in 2011. PCR amplification using diagnostic primers showed the presence of a begomovirus and subsequently the specific pair (BurNF 5’- CCATGGTTGTGGCAGTTGATTGACAGATAC-3’, BurNR 5’- CCATGGATTCACGCACAGGGGAACCC-3’) was used to amplify and clone the whole genome of the virus. The complete nucleotide sequence was determined to be 2,759 nt (accession No. HE985227). Alignments showed the highest levels of nucleotide sequence identity (98.8%) with Cotton leaf curl Burewala virus (CLCuBuV; accession No. JF416947) No. JF416947). The virus in castor beans lacks on intact C2 gene, as is typical of CLCuBuV in cotton. An amplification product of ca. 1.4 kb was obtained in PCR with primers for betasatellites and the complete nucleotide sequence of a clone was determined to be 1373 nt (HE985228). The sequence showed 96.3% nucleotide sequence identity to the recombinant Cotton leaf curl Multan betasatellite (CLCuMB; JF502389). This is the first report of CLCuBuV and its betasatellite infecting castor bean, showing this plant species as an alternate host of the virus. Already many alternate host have been reported from different alternate host like tobacco, tomato, hibiscus, okra, ageratum, Digera arvensis, habiscus, Papaya and now in Ricinus communis. So, it is suggested that these alternate hosts should be avoided to grow near cotton growing regions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ricinus%20communis" title="Ricinus communis">Ricinus communis</a>, <a href="https://publications.waset.org/abstracts/search?q=begomovirus" title=" begomovirus"> begomovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=betasatellite" title=" betasatellite"> betasatellite</a>, <a href="https://publications.waset.org/abstracts/search?q=agriculture" title=" agriculture"> agriculture</a> </p> <a href="https://publications.waset.org/abstracts/21282/isolation-and-characterization-of-cotton-infecting-begomoviruses-in-alternate-hosts-from-cotton-growing-regions-of-pakistan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21282.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">533</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">67</span> Long-Term Modal Changes in International Traffic - Example of the Polish Eastern Border</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tomasz%20Komornicki">Tomasz Komornicki</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The possibilities of cross-border traffic depend on the degree of permeability of a given border as well as the state of the existing transport infrastructure. The aim of this paper is to identify the impact of economic transformation, EU accession, and infrastructure development on modal shifts in border traffic through the Polish eastern boundary. In the 1980s railway was still the main mode of cross-border transport in Poland. At the beginning of the 1990s, the role of the road and rail transborder passenger traffic was similar, but since 1993, the role of rail was decreasing. The general decline in rail infrastructure in Poland continued uninterruptedly until accession to the European Union. The slow opposite trend can be observed later as a result of the inflow of European funds. In the countries neighbouring Poland from the east, these processes took place with some delay, and the loss of railway position was not so drastic. Therefore, cross-border railway connections have been maintained for quite a long time since the break-up of the USSR. However, finally, cross-border rail transport proved to be completely inflexible in relation to both economic, geopolitical, and transport transformations. It has been shown that the current modal split of the passenger border traffic was shaped by the following factors: a) closure of many transborder railway lines, especially local ones; b) the signing of an agreement on minor border traffic with Ukraine; c) rapidly growing number of citizens of Ukraine working in Poland (unofficial transportation of workers by car directly to their workplaces in Poland); d) the emergence of low-cost air connections between Ukraine and Poland and the growing role of air transport in the Russia-Poland relationship. The summary points to the possibility of a renewed increase in the importance of rail transport on the eastern border of the European Union. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=modal%20change" title="modal change">modal change</a>, <a href="https://publications.waset.org/abstracts/search?q=border" title=" border"> border</a>, <a href="https://publications.waset.org/abstracts/search?q=rail%20transport" title=" rail transport"> rail transport</a>, <a href="https://publications.waset.org/abstracts/search?q=Poland" title=" Poland"> Poland</a> </p> <a href="https://publications.waset.org/abstracts/138771/long-term-modal-changes-in-international-traffic-example-of-the-polish-eastern-border" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/138771.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">200</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">66</span> Treatment and Diagnostic Imaging Methods of Fetal Heart Function in Radiology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mahdi%20Farajzadeh%20Ajirlou">Mahdi Farajzadeh Ajirlou</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Prior evidence of normal cardiac anatomy is desirable to relieve the anxiety of cases with a family history of congenital heart disease or to offer the option of early gestation termination or close follow-up should a cardiac anomaly be proved. Fetal heart discovery plays an important part in the opinion of the fetus, and it can reflect the fetal heart function of the fetus, which is regulated by the central nervous system. Acquisition of ventricular volume and inflow data would be useful to quantify more valve regurgitation and ventricular function to determine the degree of cardiovascular concession in fetal conditions at threat for hydrops fetalis. This study discusses imaging the fetal heart with transvaginal ultrasound, Doppler ultrasound, three-dimensional ultrasound (3DUS) and four-dimensional (4D) ultrasound, spatiotemporal image correlation (STIC), glamorous resonance imaging and cardiac catheterization. Doppler ultrasound (DUS) image is a kind of real- time image with a better imaging effect on blood vessels and soft tissues. DUS imaging can observe the shape of the fetus, but it cannot show whether the fetus is hypoxic or distressed. Spatiotemporal image correlation (STIC) enables the acquisition of a volume of data concomitant with the beating heart. The automated volume accession is made possible by the array in the transducer performing a slow single reach, recording a single 3D data set conforming to numerous 2D frames one behind the other. The volume accession can be done in a stationary 3D, either online 4D (direct volume scan, live 3D ultrasound or a so-called 4D (3D/ 4D)), or either spatiotemporal image correlation-STIC (off-line 4D, which is a circular volume check-up). Fetal cardiovascular MRI would appear to be an ideal approach to the noninvasive disquisition of the impact of abnormal cardiovascular hemodynamics on antenatal brain growth and development. Still, there are practical limitations to the use of conventional MRI for fetal cardiovascular assessment, including the small size and high heart rate of the mortal fetus, the lack of conventional cardiac gating styles to attend data accession, and the implicit corruption of MRI data due to motherly respiration and unpredictable fetal movements. Fetal cardiac MRI has the implicit to complement ultrasound in detecting cardiovascular deformations and extracardiac lesions. Fetal cardiac intervention (FCI), minimally invasive catheter interventions, is a new and evolving fashion that allows for in-utero treatment of a subset of severe forms of congenital heart deficiency. In special cases, it may be possible to modify the natural history of congenital heart disorders. It's entirely possible that future generations will ‘repair’ congenital heart deficiency in utero using nanotechnologies or remote computer-guided micro-robots that work in the cellular layer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fetal" title="fetal">fetal</a>, <a href="https://publications.waset.org/abstracts/search?q=cardiac%20MRI" title=" cardiac MRI"> cardiac MRI</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasound" title=" ultrasound"> ultrasound</a>, <a href="https://publications.waset.org/abstracts/search?q=3D" title=" 3D"> 3D</a>, <a href="https://publications.waset.org/abstracts/search?q=4D" title=" 4D"> 4D</a>, <a href="https://publications.waset.org/abstracts/search?q=heart%20disease" title=" heart disease"> heart disease</a>, <a href="https://publications.waset.org/abstracts/search?q=invasive" title=" invasive"> invasive</a>, <a href="https://publications.waset.org/abstracts/search?q=noninvasive" title=" noninvasive"> noninvasive</a>, <a href="https://publications.waset.org/abstracts/search?q=catheter" title=" catheter"> catheter</a> </p> <a href="https://publications.waset.org/abstracts/187696/treatment-and-diagnostic-imaging-methods-of-fetal-heart-function-in-radiology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/187696.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">40</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">65</span> The Impact of FDI on Economic Growth in Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20Yagoub">Mohammed Yagoub</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The new orientation to the market economy sponsored by the Algeria government in the early Nineties of the last century, and its desire to develop investment mechanisms and the promotion of development recently, the access into a partnership with the European Union, and the forthcoming accession to the World Trade Organization, foreign direct investment makes one of the most important means of opening up to foreign markets and bring technology and interact with globalization, this article we will discuss the impact of FDI on economic growth in the Algerian. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=economic" title="economic">economic</a>, <a href="https://publications.waset.org/abstracts/search?q=development" title=" development"> development</a>, <a href="https://publications.waset.org/abstracts/search?q=markets" title=" markets"> markets</a>, <a href="https://publications.waset.org/abstracts/search?q=FDI" title=" FDI"> FDI</a>, <a href="https://publications.waset.org/abstracts/search?q=displacement" title=" displacement"> displacement</a>, <a href="https://publications.waset.org/abstracts/search?q=globalization" title=" globalization"> globalization</a> </p> <a href="https://publications.waset.org/abstracts/29895/the-impact-of-fdi-on-economic-growth-in-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29895.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">363</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">64</span> Leaf Epidermal Micromorphology as Identification Features in Accessions of Sesamum indicum L. Collected from Northern Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20D.%20Abdul">S. D. Abdul</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20B.%20J.%20Sawa"> F. B. J. Sawa</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Z.%20Andrawus"> D. Z. Andrawus</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Dan%27ilu"> G. Dan'ilu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fresh leaves of twelve accessions of S. indicum were studied to examine their stomatal features, trichomes, epidermal cell shapes and anticlinal cell-wall patterns which may be used for the delimitation of the varieties. The twelve accessions of S. indicum studied have amphistomatic leaves, i.e. having stomata on both surfaces. Four types of stomatal complex types were observed namely, diacytic, anisocytic, tetracytic and anomocytic. Anisocytic type was the most common occurring on both surfaces of all the varieties and occurred 100% in varieties lale-duk, ex-sudan and ex-gombe 6. One-way ANOVA revealed that there was no significant difference between the stomatal densities of ex-gombe 6, ex-sudan, adawa-wula, adawa-ting, ex-gombe 4 and ex-gombe 2 . Accession adawa-ting (improved) has the smallest stomatal size (26.39µm) with highest stomatal density (79.08mm2) while variety adawa-wula possessed the largest stomatal size (74.31µm) with lowest stomatal density (29.60mm2), the exception was found in variety adawa-ting whose stomatal size is larger (64.03µm) but with higher stomatal density (71.54mm2). Wavy, curve or undulate anticlinal wall patterns with irregular and or isodiametric epidermal cell shapes were observed. These accessions were found to exhibit high degree of heterogeneity in their trichome features. Ten types of trichomes were observed: unicellular, glandular peltate, capitate glandular, long unbranched uniseriate, short unbranched uniseriate, scale, multicellular, multiseriate capitate glandular, branched uniseriate and stallate trichomes. The most frequent trichome type is short-unbranched uniseriate, followed by long-unbranched uniseriate (72.73% and 72.5%) respectively. The least frequent was multiseriate capitate glandular (11.5%). The high variation in trichome types and density coupled with the stomatal complex types suggest that these varieties of S. indicum probably have the capacity to conserve water. Furthermore, the leaf micromorphological features varied from one accession to another, hence, are found to be good diagnostic and additional tool in identification as well as nomenclature of the accessions of S. indicum. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sesamum%20indicum" title="Sesamum indicum">Sesamum indicum</a>, <a href="https://publications.waset.org/abstracts/search?q=stomata" title=" stomata"> stomata</a>, <a href="https://publications.waset.org/abstracts/search?q=trichomes" title=" trichomes"> trichomes</a>, <a href="https://publications.waset.org/abstracts/search?q=epidermal%20cells" title=" epidermal cells"> epidermal cells</a>, <a href="https://publications.waset.org/abstracts/search?q=taxonomy" title=" taxonomy"> taxonomy</a> </p> <a href="https://publications.waset.org/abstracts/3845/leaf-epidermal-micromorphology-as-identification-features-in-accessions-of-sesamum-indicum-l-collected-from-northern-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3845.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">274</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">63</span> Genetic Variations of Two Casein Genes among Maghrabi Camels Reared in Egypt</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Othman%20E.%20Othman">Othman E. Othman</a>, <a href="https://publications.waset.org/abstracts/search?q=Amira%20M.%20Nowier"> Amira M. Nowier</a>, <a href="https://publications.waset.org/abstracts/search?q=Medhat%20El-Denary"> Medhat El-Denary</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Camels play an important socio-economic role within the pastoral and agricultural system in the dry and semidry zones of Asia and Africa. Camels are economically important animals in Egypt where they are dual purpose animals (meat and milk). The analysis of chemical composition of camel milk showed that the total protein contents ranged from 2.4% to 5.3% and it is divided into casein and whey proteins. The casein fraction constitutes 52% to 89% of total camel milk protein and it divided into 4 fractions namely αs1, αs2, β and κ-caseins which are encoded by four tightly genes. In spite of the important role of casein genes and the effects of their genetic polymorphisms on quantitative traits and technological properties of milk, the studies for the detection of genetic polymorphism of camel milk genes are still limited. Due to this fact, this work focused - using PCR-RFP and sequencing analysis - on the identification of genetic polymorphisms and SNPs of two casein genes in Maghrabi camel breed which is a dual purpose camel breed in Egypt. The amplified fragments at 488-bp of the camel κ-CN gene were digested with AluI endonuclease. The results showed the appearance of three different genotypes in the tested animals; CC with three digested fragments at 203-, 127- and 120-bp, TT with three digested fragments at 203-, 158- and 127-bp and CT with four digested fragments at 203-, 158-, 127- and 120-bp. The frequencies of three detected genotypes were 11.0% for CC, 48.0% for TT and 41.0% for CT genotypes. The sequencing analysis of the two different alleles declared the presence of a single nucleotide polymorphism (C→T) at position 121 in the amplified fragments which is responsible for the destruction of a restriction site (AG/CT) in allele T and resulted in the presence of two different alleles C and T in tested animals. The nucleotide sequences of κ-CN alleles C and T were submitted to GenBank with the accession numbers; KU055605 and KU055606, respectively. The primers used in this study amplified 942-bp fragments spanning from exon 4 to exon 6 of camel αS1-Casein gene. The amplified fragments were digested with two different restriction enzymes; SmlI and AluI. The results of SmlI digestion did not show any restriction site whereas the digestion with AluI endonuclease revealed the presence of two restriction sites AG^CT at positions 68^69 and 631^632 yielding the presence of three digested fragments with sizes 68-, 563- and 293-bp.The nucleotide sequences of this fragment from camel αS1-Casein gene were submitted to GenBank with the accession number KU145820. In conclusion, the genetic characterization of quantitative traits genes which are associated with the production traits like milk yield and composition is considered an important step towards the genetic improvement of livestock species through the selection of superior animals depending on the favorable alleles and genotypes; marker assisted selection (MAS). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=genetic%20polymorphism" title="genetic polymorphism">genetic polymorphism</a>, <a href="https://publications.waset.org/abstracts/search?q=SNP%20polymorphism" title=" SNP polymorphism"> SNP polymorphism</a>, <a href="https://publications.waset.org/abstracts/search?q=Maghrabi%20camels" title=" Maghrabi camels"> Maghrabi camels</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%BA-Casein%20gene" title=" κ-Casein gene"> κ-Casein gene</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1S1-Casein%20gene" title=" αS1-Casein gene"> αS1-Casein gene</a> </p> <a href="https://publications.waset.org/abstracts/45961/genetic-variations-of-two-casein-genes-among-maghrabi-camels-reared-in-egypt" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/45961.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">614</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">62</span> Phylogenetic Analysis of the Myxosporea Detected from Emaciated Olive Flounder (Paralichthys olivaceus) in Korea</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seung%20Min%20Kim">Seung Min Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Lyu%20Jin%20Jun"> Lyu Jin Jun</a>, <a href="https://publications.waset.org/abstracts/search?q=Joon%20Bum%20Jeong"> Joon Bum Jeong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Myxosporea to cause emaciation disease in the olive flounder (Paralichthys olivaceus) is a pathogen to cause severe losses in the aquafarming industry in Korea. The 3,362 bp of DNA nucleotide sequences of four myxosporean strains (EM-HM-12, EM-MA-13, EM-JJ-14, and EM-MS-15) detected by PCR method from olive flounder suffering from emaciation disease in Korea during 2012-2015 were sequenced and deposited in GenBank database (GenBank accession numbers: KU377574, KT321705, KU377575 and KU377573, respectively). The homologies of DNA nucleotide sequences of four strains were compared to each other and were more than 99.7% homologous between the four strains. All of the strains were identified as Parvicapsula petunia based on the results of phylogenetic analysis. The results in this study would be useful for the research of emaciation disease in olive flounder of Korea. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=disease" title="disease">disease</a>, <a href="https://publications.waset.org/abstracts/search?q=emaciation" title=" emaciation"> emaciation</a>, <a href="https://publications.waset.org/abstracts/search?q=olive%20flounder" title=" olive flounder"> olive flounder</a>, <a href="https://publications.waset.org/abstracts/search?q=phylogenetic%20analysis" title=" phylogenetic analysis"> phylogenetic analysis</a> </p> <a href="https://publications.waset.org/abstracts/97913/phylogenetic-analysis-of-the-myxosporea-detected-from-emaciated-olive-flounder-paralichthys-olivaceus-in-korea" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/97913.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">299</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">61</span> Blackcurrant-Associated Rhabdovirus: New Pathogen for Blackcurrants in the Baltic Sea Region</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gunta%20Resevica">Gunta Resevica</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikita%20Zrelovs"> Nikita Zrelovs</a>, <a href="https://publications.waset.org/abstracts/search?q=Ivars%20Silamikelis"> Ivars Silamikelis</a>, <a href="https://publications.waset.org/abstracts/search?q=Ieva%20Kalnciema"> Ieva Kalnciema</a>, <a href="https://publications.waset.org/abstracts/search?q=Helvijs%20Niedra"> Helvijs Niedra</a>, <a href="https://publications.waset.org/abstracts/search?q=Gun%C4%81rs%20L%C4%81cis"> Gunārs Lācis</a>, <a href="https://publications.waset.org/abstracts/search?q=Toms%20Bartulsons"> Toms Bartulsons</a>, <a href="https://publications.waset.org/abstracts/search?q=Inga%20Moro%C4%8Dko-Bi%C4%8Devska"> Inga Moročko-Bičevska</a>, <a href="https://publications.waset.org/abstracts/search?q=Arturs%20Stala%C5%BEs"> Arturs Stalažs</a>, <a href="https://publications.waset.org/abstracts/search?q=Krist%C4%ABne%20Drevinska"> Kristīne Drevinska</a>, <a href="https://publications.waset.org/abstracts/search?q=Andris%20Zeltins"> Andris Zeltins</a>, <a href="https://publications.waset.org/abstracts/search?q=Ina%20Balke"> Ina Balke</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Newly discovered viruses provide novel knowledge for basic phytovirus research, serve as tools for biotechnology and can be helpful in identification of epidemic outbreaks. Blackcurrant-associated rhabdovirus (BCaRV) have been discovered in USA germplasm collection samples from Russia and France. As it was reported in one accession originating from France it is unclear whether the material was already infected when it entered in the USA or it became infected while in collection in the USA. Due to that BCaRV was definite as non-EU viruses. According to ICTV classification BCaRV is representative of Blackcurrant betanucleorhabdovirus specie in genus Betanucleorhabdovirus (family Rhabdoviridae). Nevertheless, BCaRV impact on the host, transmission mechanisms and vectors are still unknown. In RNA-seq data pool from Ribes plants resistance gene study by high throughput sequencing (HTS) we observed differences between sample group gene transcript heat maps. Additional analysis of the whole data pool (total 393660492 of 150 bp long read pairs) by rnaSPAdes v 3.13.1 resulted into 14424 bases long contig with an average coverage of 684x with shared 99.5% identity to the previously reported first complete genome of BCaRV (MF543022.1) using EMBOSS Needle. This finding proved BCaRV presence in EU and indicated that it might be relevant pathogen. In this study leaf tissue from twelve asymptomatic blackcurrant cv. Mara Eglite plants (negatively tested for blackcurrant reversion virus (BRV)) from Dobele, Latvia (56°36'31.9"N, 23°18'13.6"E) was collected and used for total RNA isolation with RNeasy Plant Mini Kit with minor modifications, followed by plant rRNA removal by a RiboMinus Plant Kit for RNA-Seq. HTS libraries were prepared using MGI Easy RNA Directional Library Prep Set for 16 reactions to obtain 150 bp pair-end reads. Libraries were pooled, circularized and cleaned and sequenced on DNBSEQ-G400 using PE150 flow cell. Additionally, all samples were tested by RT-PCR, and amplicons were directly sequenced by Sanger-based method. The contig representing the genome of BCaRV isolate Mara Eglite was deposited at European Nucleotide Archive under accession number OU015520. Those findings indicate a second evidence on the presence of this particular virus in the EU and further research on BCaRV prevalence in Ribes from other geographical areas should be performed. As there are no information on BCaRV impact on the host this should be investigated, regarding the fact that mixed infections with BRV and nucleorhabdoviruses are reported. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=BCaRV" title="BCaRV">BCaRV</a>, <a href="https://publications.waset.org/abstracts/search?q=Betanucleorhabdovirus" title=" Betanucleorhabdovirus"> Betanucleorhabdovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=Ribes" title=" Ribes"> Ribes</a>, <a href="https://publications.waset.org/abstracts/search?q=RNA-seq" title=" RNA-seq"> RNA-seq</a> </p> <a href="https://publications.waset.org/abstracts/141962/blackcurrant-associated-rhabdovirus-new-pathogen-for-blackcurrants-in-the-baltic-sea-region" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141962.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">184</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">60</span> Analysis of the Evolution of Social and Economic Indicators of the Mercosur´s Members: 1980-2012</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=L.%20Aparecida%20Bastos">L. Aparecida Bastos</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Leige%20Lopes"> J. Leige Lopes</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Crepaldi"> J. Crepaldi</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Monteiro%20da%20Silva"> R. Monteiro da Silva </a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of this study is to analyze the evolution of some social and economic indicators of Mercosur´s economies from 1980 to 2012, based on the statistics of the Latin American Integration Association (LAIA). The objective is to observe if after the accession of these economies to Mercosur (the first accessions occurred in 1994) these indicators showed better performance, in order to demonstrate if economic integration contributed to improved trade, macroeconomic performance, and level of social and economic development of member countries. To this end, the methodologies used will be a literature review and descriptive statistics. The theoretical framework that guides the work are the theories of Integration: Classical Liberal, Marxist and structural-proactive. The results reveal that most social and economic indicators showed better performance in those economies that joined Mercosur after 1994. This work is the result of an investigation already completed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=economic%20integration" title="economic integration">economic integration</a>, <a href="https://publications.waset.org/abstracts/search?q=Mercosur" title=" Mercosur"> Mercosur</a>, <a href="https://publications.waset.org/abstracts/search?q=social%20indicators" title=" social indicators"> social indicators</a>, <a href="https://publications.waset.org/abstracts/search?q=economic%20indicators" title=" economic indicators"> economic indicators</a> </p> <a href="https://publications.waset.org/abstracts/47883/analysis-of-the-evolution-of-social-and-economic-indicators-of-the-mercosurs-members-1980-2012" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47883.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">298</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">59</span> Promissing Antifungal Chitinase from Marine Strain of Bacillus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ben%20Amar%20Cheba">Ben Amar Cheba</a>, <a href="https://publications.waset.org/abstracts/search?q=Taha%20Ibrahim%20Zaghloul"> Taha Ibrahim Zaghloul</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamad%20Hisham%20El-Massry"> Mohamad Hisham El-Massry</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmad%20Rafik%20El-Mahdy"> Ahmad Rafik El-Mahdy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Seventy two bacterial strains with ability to degrade chitin were isolated during a screening program. One of the most potent isolates (strain R2) was identified as Bacillus sp. using conventional methods as well as 16S rRNA technique and submitted in the Gen Bank sequence database as Bacillus sp. R2 with a given accession number DQ 923161. This strain was able to produce high levels of extracellular chitinase. The chitinase of Bacillus sp. R2 hydrolyzed several chitinous substrates preferentially and showed a maximum activity toward the β chitin such as Calmar pen and squid bone chitins with the folds 1.47 and 1.23 respectively. The enzyme also exhibited a substrate binding capacity of more than 70% for squid chitin, shrimp shell colloidal chitin, chitosan and prawn shell chitin. The chitinase showed a moderate antifungal activity against many phytopathogenic fungi such as Aspergillus niger, A. flavus, Penicillium degitatum and Fusarium calmorum.This strain could be a suitable candidate for chitinase production on an industrial scale for using as promising antifungal biopestecide. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antifungal%20activity" title="antifungal activity">antifungal activity</a>, <a href="https://publications.waset.org/abstracts/search?q=Bacillus%20sp.%20R2" title=" Bacillus sp. R2"> Bacillus sp. R2</a>, <a href="https://publications.waset.org/abstracts/search?q=chitinase" title=" chitinase"> chitinase</a>, <a href="https://publications.waset.org/abstracts/search?q=substrate%20specificity" title=" substrate specificity "> substrate specificity </a> </p> <a href="https://publications.waset.org/abstracts/26781/promissing-antifungal-chitinase-from-marine-strain-of-bacillus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26781.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">501</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">58</span> Possible Involvement of DNA-methyltransferase and Histone Deacetylase in the Regulation of Virulence Potential of Acanthamoeba castellanii</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yi%20H.%20Wong">Yi H. Wong</a>, <a href="https://publications.waset.org/abstracts/search?q=Li%20L.%20Chan"> Li L. Chan</a>, <a href="https://publications.waset.org/abstracts/search?q=Chee%20O.%20Leong"> Chee O. Leong</a>, <a href="https://publications.waset.org/abstracts/search?q=Stephen%20Ambu"> Stephen Ambu</a>, <a href="https://publications.waset.org/abstracts/search?q=Joon%20W.%20Mak"> Joon W. Mak</a>, <a href="https://publications.waset.org/abstracts/search?q=Priyadashi%20S.%20Sahu"> Priyadashi S. Sahu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Acanthamoeba is a free-living opportunistic protist which is ubiquitously distributed in the environment. Virulent Acanthamoeba can cause fatal encephalitis in immunocompromised patients and potential blinding keratitis in immunocompetent contact lens wearers. Approximately 24 species have been identified but only the A. castellanii, A. polyphaga and A. culbertsoni are commonly associated with human infections. Until to date, the precise molecular basis for Acanthamoeba pathogenesis remains unclear. Previous studies reported that Acanthamoeba virulence can be diminished through prolonged axenic culture but revived through serial mouse passages. As no clear explanation on this reversible pathogenesis is established, hereby, we postulate that the epigenetic regulators, DNA-methyltransferases (DNMT) and histone-deacetylases (HDAC), could possibly be involved in granting the virulence plasticity of Acanthamoeba spp. Methods: Four rounds of mouse passages were conducted to revive the virulence potential of the virulence-attenuated Acanthamoeba castellanii strain (ATCC 50492). Briefly, each mouse (n=6/group) was inoculated intraperitoneally with Acanthamoebae cells (2x 105 trophozoites/mouse) and incubated for 2 months. Acanthamoebae cells were isolated from infected mouse organs by culture method and subjected to subsequent mouse passage. In vitro cytopathic, encystment and gelatinolytic assays were conducted to evaluate the virulence characteristics of Acanthamoebae isolates for each passage. PCR primers which targeted on the 2 members (DNMT1 and DNMT2) and 5 members (HDAC1 to 5) of the DNMT and HDAC gene families respectively were custom designed. Quantitative real-time PCR (qPCR) was performed to detect and quantify the relative expression of the two gene families in each Acanthamoeba isolates. Beta-tubulin of A. castellanii (Genbank accession no: XP_004353728) was included as housekeeping gene for data normalisation. PCR mixtures were also analyzed by electrophoresis for amplicons detection. All statistical analyses were performed using the paired one-tailed Student’s t test. Results: Our pathogenicity tests showed that the virulence-reactivated Acanthamoeba had a higher degree of cytopathic effect on vero cells, a better resistance to encystment challenge and a higher gelatinolytic activity which was catalysed by serine protease. qPCR assay showed that DNMT1 expression was significantly higher in the virulence-reactivated compared to the virulence-attenuated Acanthamoeba strain (p ≤ 0.01). The specificity of primers which targeted on DNMT1 was confirmed by sequence analysis of PCR amplicons, which showed a 97% similarity to the published DNA-methyltransferase gene of A. castellanii (GenBank accession no: XM_004332804.1). Out of the five primer pairs which targeted on the HDAC family genes, only HDAC4 expression was significantly difference between the two variant strains. In contrast to DNMT1, HDAC4 expression was much higher in the virulence-attenuated Acanthamoeba strain. Conclusion: Our mouse passages had successfully restored the virulence of the attenuated strain. Our findings suggested that DNA-methyltransferase (DNMT1) and histone deacetylase (HDAC4) expressions are associated with virulence potential of Acanthamoeba spp. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acanthamoeba" title="acanthamoeba">acanthamoeba</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA-methyltransferase" title=" DNA-methyltransferase"> DNA-methyltransferase</a>, <a href="https://publications.waset.org/abstracts/search?q=histone%20deacetylase" title=" histone deacetylase"> histone deacetylase</a>, <a href="https://publications.waset.org/abstracts/search?q=virulence-associated%20proteins" title=" virulence-associated proteins"> virulence-associated proteins</a> </p> <a href="https://publications.waset.org/abstracts/49185/possible-involvement-of-dna-methyltransferase-and-histone-deacetylase-in-the-regulation-of-virulence-potential-of-acanthamoeba-castellanii" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/49185.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">289</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">57</span> Identification and Differentiation of Fagonia Arabica and Fagonia Indica by Using DNA Barcode Region Matk</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Noshaba%20Dilbar">Noshaba Dilbar</a>, <a href="https://publications.waset.org/abstracts/search?q=Aisha%20Tahir"> Aisha Tahir</a>, <a href="https://publications.waset.org/abstracts/search?q=Amer%20Jamil"> Amer Jamil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> During the last decade, DNA barcoding proved to be an authentic tool for discovery and identification of plants. In the present study, DNA barcoding of two species, Fagonia arabica and Fagonia indica was done for differentiation by using matK region. matK gene is considered as a universal barcode because of its easy alignment and high discrimination ability. In this study, matK yielded 100% sequencing results. The sequences from both plants were aligned at clustal W and observed that there is no nucleotide variation and polymorphism among both sequences. This was further analysed by BLAST which showed the similar sequences from different plants belonging to same family but didn’t find sequence of both species. Considering this, the resulted sequence was submitted by the name of Fagonia arabica with accession number KM276890. In the end, we analysed the results from BOLD which gave us the final conclusion that both plants are same as their matK sequences are 100% identical. In literature, both Fagonia indica and Fagonia arabica names are used for this plant but there is no clear differentiation has been observed in these plants. Results evaluate that Fagonia indica and Fagonia arabica are the alternative names of same plant. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DNA%20barcoding" title="DNA barcoding">DNA barcoding</a>, <a href="https://publications.waset.org/abstracts/search?q=Fagonia%20arabica" title=" Fagonia arabica"> Fagonia arabica</a>, <a href="https://publications.waset.org/abstracts/search?q=Fagonia%20indica" title=" Fagonia indica"> Fagonia indica</a>, <a href="https://publications.waset.org/abstracts/search?q=matK" title=" matK"> matK</a> </p> <a href="https://publications.waset.org/abstracts/122682/identification-and-differentiation-of-fagonia-arabica-and-fagonia-indica-by-using-dna-barcode-region-matk" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/122682.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">153</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">56</span> Gene Cloning and Expression of Azoreductases from Azo-Degraders Lysinibacillus macrolides and Bacillus coagulans Isolated from Egyptian Industrial Wastewater</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Omaima%20A.%20Sharaf">Omaima A. Sharaf</a>, <a href="https://publications.waset.org/abstracts/search?q=Wafaa%20M.%20Abd%20El-Rahim"> Wafaa M. Abd El-Rahim</a>, <a href="https://publications.waset.org/abstracts/search?q=Hassan%20Moawad"> Hassan Moawad</a>, <a href="https://publications.waset.org/abstracts/search?q=Michael%20J.%20Sadowsky"> Michael J. Sadowsky</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Textile industry is one of the important industries in the worldwide. It is known that the eco-friendly industrial and agricultural activities are significant for socio-economic stability of all countries. The absence of appropriate industrial waste water treatments is essential barrier for sustainable development in food and agricultural sectors especially in developing country like Egypt. Thus, the development of enzymatic bioremediation technology for textile dye removal will enhance the collaboration between scientists who develop the technology and industry where this technology will be implemented towards the safe disposal of the textile dye wastes. Highly efficient microorganisms are of most importance in developing and using highly effective biological treatment processes. Bacterial degradation of azo dyes is generally initiated by an enzymatic step that involves cleavage of azo linkages, usually with the aid of an azoreductase as electron donor. Thus, expanding the spectrum of microorganisms with high enzymatic activities as azoreductases and discovering novel azo-dye degrading enzymes, with enhanced stability and superior catalytic properties, are necessary for many environmental and industrial applications. Consequently, the use of molecular tools has become increasingly integrated into the understanding of enzyme properties and characterization. Researchers have utilized a gene cloning and expression methods as a tool to produce recombinant protein for decolorizing dyes more efficiently. Thus, presumptive evidence for the presence of genes encoding azoreductases in the genomes of selected local, and most potent azo-degrading strains were obtained by using specific oligonucleotides primers. These potent strains have been isolated from textile industrial wastewater in Egypt and identified using 16S rRNA sequence analysis as 'Lysinibacillus macrolidesB8, Brevibacillus parabrevisB11, Bacillus coagulansB7, and B. cereusB5'. PCR products of two full length genes designated as (AZO1;621bp and AZO2;534bp) were detected. BLASTx results indicated that AZO1 gene was corresponding to predicted azoreductase from of Bacillus sp. ABP14, complete genome, multispecies azoreductase [Bacillus], It was submitted to the gene bank by an accession no., BankIt2085371 AZO1 MG923210 (621bp; 207 amino acids). AZO1 was generated from the DNA of our identified strains Lysinibacillus macrolidesB8. On the other hand, AZO2 gene was corresponding to a predicted azoreductase from Bacillus cereus strain S2-8. Gene bank accession no. was BankIt2085839 AZO2 MG932081 (534bp;178 amino acids) and it was amplified from our Bacillus coagulansB7. Both genes were successfully cloned into pCR2.1TOPO (Invitrogen) and in pET28b+ vectors, then they transformed into E. coli DH5α and BL21(DE3) cells for heterologous expression studies. Our recombinant azoreductases (AZO1&AZO2) exhibited potential enzyme activity and efficiently decolorized an azo dye (Direct violet). They exhibited pH stability between 6 and 8 with optimum temperature up to 60°C and 37 °C after induction by 1mM and 1.5mM IPTG, for both AZO1 &AZO2, respectively. These results suggested that further optimization and purification of these recombinant proteins by using different heterologous expression systems will give great potential for the sustainable utilization of these recombinant enzymes in several industrial applications especially in wastewater treatments. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=azoreductases" title="azoreductases">azoreductases</a>, <a href="https://publications.waset.org/abstracts/search?q=decolorization" title=" decolorization"> decolorization</a>, <a href="https://publications.waset.org/abstracts/search?q=enzyme%20activity" title=" enzyme activity"> enzyme activity</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20cloning%20and%20expression" title=" gene cloning and expression"> gene cloning and expression</a> </p> <a href="https://publications.waset.org/abstracts/96859/gene-cloning-and-expression-of-azoreductases-from-azo-degraders-lysinibacillus-macrolides-and-bacillus-coagulans-isolated-from-egyptian-industrial-wastewater" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96859.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">129</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">55</span> Quality and Yield of Potato Seed Tubers as Influenced by Plant Growth Promoting Rhizobacteria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Raqib%20Rasul">Muhammad Raqib Rasul</a>, <a href="https://publications.waset.org/abstracts/search?q=Tavga%20Sulaiman%20Rashid"> Tavga Sulaiman Rashid</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fertilization increases efficiency and obtains better quality of product recovery in agricultural activities. However, fertilizer consumption increased exponentially throughout the world, causing severe environmental problems. Biofertilizers can be a practical approach to minimize chemical fertilizer sources and ultimately develop soil fertility. This study was carried out to isolate, identify and characterize bacteria from medicinal plant (Rumex tuberosus L. and Verbascum sp.) rhizosphere for in vivo screening. 25 bacterial isolates were isolated and several biochemical tests were performed. Two isolates that were positive for most biochemical tests were chosen for the field experiment. The isolates were identified as Go1 Alcaligenes faecalis (Accession No. OP001725) and T11 (Bacillus sp.) based on the 16S rRNA sequence analysis that was compared with related bacteria in GenBank database using MEGA 6.1. For the field trial isolate GO1 and T11 (separately and mixed), NPK as a positive control was used. Both isolates increased plant height, chlorophyll content, number of tubers, and tuber’s weight. The results demonstrated that these two isolates of bacteria can potentially replace with chemical fertilizers for potato production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biofertilizer" title="biofertilizer">biofertilizer</a>, <a href="https://publications.waset.org/abstracts/search?q=Bacillus%20subtilis" title=" Bacillus subtilis"> Bacillus subtilis</a>, <a href="https://publications.waset.org/abstracts/search?q=Alcaligenes%20faecalis" title=" Alcaligenes faecalis"> Alcaligenes faecalis</a>, <a href="https://publications.waset.org/abstracts/search?q=potato%20tubers" title=" potato tubers"> potato tubers</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vivo%20screening" title=" in vivo screening"> in vivo screening</a> </p> <a href="https://publications.waset.org/abstracts/155470/quality-and-yield-of-potato-seed-tubers-as-influenced-by-plant-growth-promoting-rhizobacteria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/155470.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">103</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">54</span> The Impact of the Interest Rates on Investments in the Context of Financial Crisis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Joanna%20Stawska">Joanna Stawska</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The main objective of this article is to examine the impact of interest rates on investments in Poland in the context of financial crisis. The paper also investigates the dependence of bank loans to enterprises on interbank market rates. The article studies the impact of interbank market rate on the level of investments in Poland. Besides, this article focuses on the research of the correlation between the level of corporate loans and the amount of investments in Poland in order to determine the indirect impact of central bank interest rates through the transmission mechanism of monetary policy on the real economy. To achieve the objective we have used econometric and statistical research methods like: econometric model and Pearson correlation coefficient. This analysis suggests that the central bank reference rate inversely proportionally affects the level of investments in Poland and this dependence is moderate. This is also important issue because it is related to preparing of Poland to accession to euro area. The research is important from both theoretical and empirical points of view. The formulated conclusions and recommendations determine the practical significance of the paper which may be used in the decision making process of monetary and economic authorities of the country. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=central%20bank" title="central bank">central bank</a>, <a href="https://publications.waset.org/abstracts/search?q=financial%20crisis" title=" financial crisis"> financial crisis</a>, <a href="https://publications.waset.org/abstracts/search?q=interest%20rate" title=" interest rate"> interest rate</a>, <a href="https://publications.waset.org/abstracts/search?q=investments" title=" investments"> investments</a> </p> <a href="https://publications.waset.org/abstracts/22155/the-impact-of-the-interest-rates-on-investments-in-the-context-of-financial-crisis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22155.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">442</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">53</span> Isolation, Identification and Crude Oil Biodegradation Potential of Providencia sp. BAZ 01</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aisami%20A.">Aisami A.</a>, <a href="https://publications.waset.org/abstracts/search?q=Z.%20A.%20Adamu"> Z. A. Adamu</a>, <a href="https://publications.waset.org/abstracts/search?q=Lawan%20Bulama"> Lawan Bulama</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Due to growing issues of crude oil pollution in both marine and terrestrial environments, Billions to Trillions of US Dollars were spent over the years for the treatment of this spill. There is an urgent need for effective bioremediation strategies. This current study focuses on the isolation and characterization of a crude oil-degrading bacterium from hydrocarbon-contaminated soil samples. Soil samples were collected from an oil spill site and subjected to enrichment culture techniques in a mineral salt medium supplemented with crude oil as the singular carbon source. The isolates were screened for their crude oil-degrading capabilities using gravimetric analysis. The most efficient isolation was identified through 16S rRNA gene sequencing. Cultural and physical conditions such pH, temperature salinity and crude oil concentrations were optimized. The isolates showed significant crude oil degradation efficiency, reducing oil concentration (2.5%) by 75% within 15 days of incubation. The strain was identified as Providencia sp. through molecular characterization, the sequence was deposited at the NCBI Genbank with accession number MN880494. The bacterium exhibited optimal growth at 32.5°C, pH 7.0 to 7.5, and in the presence of 1.5% (w/v) NaCl. The isolated Providencia sp. shows encouraging potential for bioremediation of crude oil-contaminated environments. This study successfully isolated and characterized a crude oil-degrading Providencia sp., highlighting its potential in bioremediation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=crude%20oil%20degradation" title="crude oil degradation">crude oil degradation</a>, <a href="https://publications.waset.org/abstracts/search?q=providencia%20sp." title=" providencia sp."> providencia sp.</a>, <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title=" bioremediation"> bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrocarbon%20utilization" title=" hydrocarbon utilization"> hydrocarbon utilization</a>, <a href="https://publications.waset.org/abstracts/search?q=environmental%20pollution." title=" environmental pollution."> environmental pollution.</a> </p> <a href="https://publications.waset.org/abstracts/188258/isolation-identification-and-crude-oil-biodegradation-potential-of-providencia-sp-baz-01" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188258.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">42</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">52</span> Cloning, Expression and Protein Purification of AV1 Gene of Okra Leaf Curl Virus Egyptian Isolate and Genetic Diversity between Whitefly and Different Plant Hosts</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dalia.%20G.%20Aseel">Dalia. G. Aseel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <em>Begomoviruses</em> are economically important plant viruses that infect dicotyledonous plants and exclusively transmitted by the whitefly <em>Bemisia tabaci. Here, replicative form was isolated from Okra, Cotton, Tomato plants and whitefly infected with Begomoviruses. Using coat protein specific primers (AV1), the viral infection was verified with amplicon at 450 bp. </em>The sequence of OLCuV-AV1 gene was recorded and received an accession number (FJ441605) from Genebank. The phylogenetic tree of OLCuV was closely related to <em>Okra leaf curl virus </em>previously isolated from Cameroon and USA with nucleotide sequence identity of 92%. The protein purification was carried out using His-Tag methodology by using Affinity Chromatography. The purified protein was separated on SDS-PAGE analysis and an enriched expected size of band at 30 kDa was observed. Furthermore, RAPD and SDS-PAGE were used to detect genetic variability between different hosts of <em>okra leaf curl virus</em> (OLCuV), <em>cotton leaf curl virus</em> (CLCuV), <em>tomato yellow leaf curl virus</em> (TYLCuV) and the whitefly vector. Finally, the present study would help to understand the relationship between the whitefly and different economical crops in Egypt. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=okra%20leaf%20curl%20virus" title="okra leaf curl virus">okra leaf curl virus</a>, <a href="https://publications.waset.org/abstracts/search?q=AV1%20gene" title=" AV1 gene"> AV1 gene</a>, <a href="https://publications.waset.org/abstracts/search?q=sequencing" title=" sequencing"> sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=phylogenetic" title=" phylogenetic"> phylogenetic</a>, <a href="https://publications.waset.org/abstracts/search?q=cloning" title=" cloning"> cloning</a>, <a href="https://publications.waset.org/abstracts/search?q=purified%20protein" title=" purified protein"> purified protein</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20diversity%20and%20viral%20proteins" title=" genetic diversity and viral proteins"> genetic diversity and viral proteins</a> </p> <a href="https://publications.waset.org/abstracts/91013/cloning-expression-and-protein-purification-of-av1-gene-of-okra-leaf-curl-virus-egyptian-isolate-and-genetic-diversity-between-whitefly-and-different-plant-hosts" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91013.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">148</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">51</span> Identification and Classification of Gliadin Genes in Iranian Diploid Wheat</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jafar%20Ahmadi">Jafar Ahmadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Alireza%20Pour-Aboughadareh"> Alireza Pour-Aboughadareh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Wheat is the first and the most important grain of the world and its bakery property is due to glutenin and gliadin qualities. Wheat seed proteins were divided into four groups according to solubility. Two groups are albumin and globulin dissolving in water and salt solutions possessing metabolic activities. Two other groups are inactive and non-dissolvable and contain glutelins or glutenins and prolamins or gliadins. Gliadins are major components of the storage proteins in wheat endosperm. Gliadin proteins are separated into three groups based on electrophoretic mobility: α/β-gliadin, γ-gliadin, and ω-gliadin. It seems that little information is available about gliadin genes in Iranian wild relatives of wheat. Thus, the aim of this study was the evaluation of the wheat wild relatives collected from different origins of Zagros Mountains in Iran, involving coding gliadin genes using specific primers. For this, forty accessions of Triticum boeoticum and Triticum urartu were selected. For each accession, genomic DNA was extracted and PCRs were performed in total volumes of 15 μl. The amplification products were separated on 1.5% agarose gels. In results, for Gli-2A locus, three allelic variants were detected by Gli-2As primer pairs. The sizes of PCR products for these alleles were 210, 490 and 700 bp. Only five (13%) and two accessions (5%) produced 700 and 490 bp fragments when their DNA was ampliﬁed with the Gli.As.2 primer pairs. However, 37 of the 40 accessions (93%) carried 210 bp allele, and three accessions (8%) did not yield any product for this marker. Therefore, these germplasm could be used as rich gene pool to broaden the genetic base of bread wheat. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diploied%20wheat" title="diploied wheat">diploied wheat</a>, <a href="https://publications.waset.org/abstracts/search?q=gliadin" title=" gliadin"> gliadin</a>, <a href="https://publications.waset.org/abstracts/search?q=Triticum%20boeoticum" title=" Triticum boeoticum"> Triticum boeoticum</a>, <a href="https://publications.waset.org/abstracts/search?q=Triticum%20urartu" title=" Triticum urartu"> Triticum urartu</a> </p> <a href="https://publications.waset.org/abstracts/38120/identification-and-classification-of-gliadin-genes-in-iranian-diploid-wheat" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/38120.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">251</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">50</span> Isolation of a Bacterial Community with High Removal Efficiencies of the Insecticide Bendiocarb</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eusebio%20A.%20Jim%C3%A9nez-Ar%C3%A9valo">Eusebio A. Jiménez-Arévalo</a>, <a href="https://publications.waset.org/abstracts/search?q=Deifilia%20Ahuatzi-Chac%C3%B3n"> Deifilia Ahuatzi-Chacón</a>, <a href="https://publications.waset.org/abstracts/search?q=Juvencio%20Gal%C3%ADndez-Mayer"> Juvencio Galíndez-Mayer</a>, <a href="https://publications.waset.org/abstracts/search?q=Cleotilde%20Ju%C3%A1rez-Ram%C3%ADrez"> Cleotilde Juárez-Ramírez</a>, <a href="https://publications.waset.org/abstracts/search?q=Nora%20Ruiz-Ordaz"> Nora Ruiz-Ordaz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bendiocarb is a known toxic xenobiotic that presents acute and chronic risks for freshwater invertebrates and estuarine and marine biota; thus, the treatment of water contaminated with the insecticide is of concern. In this paper, a bacterial community with the capacity to grow in bendiocarb as its sole carbon and nitrogen source was isolated by enrichment techniques in batch culture, from samples of a composting plant located in the northeast of Mexico City. Eight cultivable bacteria were isolated from the microbial community, by PCR amplification of 16 rDNA; <em>Pseudoxanthomonas spadix</em> (NC_016147.2, 98%), <em>Ochrobacterium anthropi</em> (NC_009668.1, 97%), <em>Staphylococcus capitis</em> (NZ_CP007601.1, 99%), <em>Bosea thiooxidans</em>. (NZ_LMAR01000067.1, 99%), <em>Pseudomonas denitrificans</em>. (NC_020829.1, 99%), <em>Agromyces </em>sp. (NZ_LMKQ01000001.1, 98%), <em>Bacillus thuringiensis</em>. (NC_022873.1, 97%), <em>Pseudomonas alkylphenolia </em>(NZ_CP009048.1, 98%). NCBI accession numbers and percentage of similarity are indicated in parentheses. These bacteria were regarded as the isolated species for having the best similarity matches. The ability to degrade bendiocarb by the immobilized bacterial community in a packed bed biofilm reactor, using as support volcanic stone fragments (tezontle), was evaluated. The reactor system was operated in batch using mineral salts medium and 30 mg/L of bendiocarb as carbon and nitrogen source. With this system, an overall removal efficiency (η<sub>bend</sub>) rounding 90%, was reached. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bendiocarb" title="bendiocarb">bendiocarb</a>, <a href="https://publications.waset.org/abstracts/search?q=biodegradation" title=" biodegradation"> biodegradation</a>, <a href="https://publications.waset.org/abstracts/search?q=biofilm%20reactor" title=" biofilm reactor"> biofilm reactor</a>, <a href="https://publications.waset.org/abstracts/search?q=carbamate%20insecticide" title=" carbamate insecticide"> carbamate insecticide</a> </p> <a href="https://publications.waset.org/abstracts/55702/isolation-of-a-bacterial-community-with-high-removal-efficiencies-of-the-insecticide-bendiocarb" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/55702.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">279</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">49</span> Art, Nature, and City in the Construction of Contemporary Public Space</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rodrigo%20Coelho">Rodrigo Coelho</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We believe that in the majority of the “recent production of public space", the overvaluation of the "image", of the "ephemeral" and of the "objectual", has come to determine the configuration of banal and (more or less) arbitrary "public spaces", mostly linked to a problem of “outdoor decoration”, reflecting a clear sign of uncertainty and arbitrariness about the meaning, the role and shape of public space and public art.This "inconsistency" which is essentially linked to the loss of urban, but also social, cultural and political, vocation of the disciplines that “shape” the urban space (but is also linked to the lack of urban and technical culture of techinicians and policy makers) converted a significant set of the recently built "public space" and “urban art” into diffuse and multi-referenced pieces, which generally shares the inability of confering to the urban space, civic, aesthetic, social and symbolic meanings. In this sense we consider it is essential to undertake a theoretical reflection on the values, the meaning(s) and the shape(s) that open space, and urban art may (or must) take in the current urban and cultural context, in order to redeem for public space its status of significant physical reference, able to embody a spatial and urban identity, and simultaneously enable the collective accession and appropriation of public space. Taking as reference public space interventions built in the last decade on the European context, we will seek to explore and defend the need of considering public space as a true place of exception, an exceptional support where the emphasis is placed on the quality of the experience, especially by the relations public space/urban art can established with the city, with nature and geography in a broad sense, referring us back to a close and inseparable and timeless relationship between nature and culture. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=art" title="art">art</a>, <a href="https://publications.waset.org/abstracts/search?q=city" title=" city"> city</a>, <a href="https://publications.waset.org/abstracts/search?q=nature" title=" nature"> nature</a>, <a href="https://publications.waset.org/abstracts/search?q=public%20space" title=" public space"> public space</a> </p> <a href="https://publications.waset.org/abstracts/22818/art-nature-and-city-in-the-construction-of-contemporary-public-space" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22818.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">449</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=accession&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=accession&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=accession&page=2" rel="next">&rsaquo;</a></li> </ul> 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