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window.loswp.shouldDetectTimezone = true; window.loswp.shouldShowBulkDownload = true; window.loswp.showSignupCaptcha = false window.loswp.willEdgeCache = false; window.loswp.work = {"work":{"id":20498269,"created_at":"2016-01-22T01:58:18.968-08:00","from_world_paper_id":146515384,"updated_at":"2024-11-27T19:47:23.989-08:00","_data":{"ai_title_tag":"Porous Hydroxyapatite Ceramics Enhance Osteoblast Growth In Vitro","grobid_abstract":"Blocks of two porous synthetic hydroxyapatites (HA) with porosity fraction of 30 Á/40 and 50 Á/60 vol%, respectively and a coralline derived porous HA were evaluated in vitro in presence of the osteogenic line MC3T3-E1 and of L929 fibroblasts. The two tested biomaterials did not affect cellular proliferation (MTT test), but the contact inhibited alkaline phosphatase activity. Porous aggregates resulted perfectly biocompatible in the tests performed, since observations performed by light microscopy did not show any cell morphological change, osteoblast presented a stellar shape and typical pseudopodes. SEM observations showed intercellular matrix containing fibers on HA-based porous aggregates. #","publication_date":"2002,,","publication_name":"Biomolecular Engineering","grobid_abstract_attachment_id":"41404861"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Growth of osteoblast-like cells on porous hydroxyapatite ceramics: an in vitro study","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [42474837,27140242]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loginModal = {}; window.loginModal.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":41404861,"attachmentType":"pdf"}"><img alt="First page of “Growth of osteoblast-like cells on porous hydroxyapatite ceramics: an in vitro study”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/41404861/mini_magick20190219-16655-y3qkui.png?1550594877" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/images/single_work_splash/adobe_icon.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Growth of osteoblast-like cells on porous hydroxyapatite ceramics: an in vitro study</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="42474837" href="https://independent.academia.edu/LCerroni"><img alt="Profile image of L. Cerroni" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />L. Cerroni</a><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="27140242" href="https://independent.academia.edu/CorradoPiconi"><img alt="Profile image of Corrado Piconi" class="ds-work-card--author-avatar" src="//a.academia-assets.com/images/s65_no_pic.png" />Corrado Piconi</a></div><div class="ds-work-card--detail"><p class="ds-work-card--detail ds2-5-body-sm">2002, Biomolecular Engineering</p><div class="ds-work-card--work-metadata"><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">visibility</span><p class="ds2-5-body-sm" id="work-metadata-view-count">…</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">description</span><p class="ds2-5-body-sm">6 pages</p></div><div class="ds-work-card--work-metadata__stat"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">link</span><p class="ds2-5-body-sm">1 file</p></div></div><script>(async () => { const workId = 20498269; const worksViewsPath = "/v0/works/views?subdomain_param=api&work_ids%5B%5D=20498269"; const getWorkViews = async (workId) => { const response = await fetch(worksViewsPath); if (!response.ok) { throw new Error('Failed to load work views'); } const data = await response.json(); return data.views[workId]; }; // Get the view count for the work - we send this immediately rather than waiting for // the DOM to load, so it can be available as soon as possible (but without holding up // the backend or other resource requests, because it's a bit expensive and not critical). const viewCount = await getWorkViews(workId); const updateViewCount = (viewCount) => { try { const viewCountNumber = parseInt(viewCount, 10); if (viewCountNumber === 0) { // Remove the whole views element if there are zero views. document.getElementById('work-metadata-view-count')?.parentNode?.remove(); return; } const commaizedViewCount = viewCountNumber.toLocaleString(); const viewCountBody = document.getElementById('work-metadata-view-count'); if (!viewCountBody) { throw new Error('Failed to find work views element'); } viewCountBody.textContent = `${commaizedViewCount} views`; } catch (error) { // Remove the whole views element if there was some issue parsing. document.getElementById('work-metadata-view-count')?.parentNode?.remove(); throw new Error(`Failed to parse view count: ${viewCount}`, error); } }; // If the DOM is still loading, wait for it to be ready before updating the view count. if (document.readyState === "loading") { document.addEventListener('DOMContentLoaded', () => { updateViewCount(viewCount); }); // Otherwise, just update it immediately. } else { updateViewCount(viewCount); } })();</script></div><p class="ds-work-card--work-abstract ds-work-card--detail ds2-5-body-md">Blocks of two porous synthetic hydroxyapatites (HA) with porosity fraction of 30 Á/40 and 50 Á/60 vol%, respectively and a coralline derived porous HA were evaluated in vitro in presence of the osteogenic line MC3T3-E1 and of L929 fibroblasts. The two tested biomaterials did not affect cellular proliferation (MTT test), but the contact inhibited alkaline phosphatase activity. Porous aggregates resulted perfectly biocompatible in the tests performed, since observations performed by light microscopy did not show any cell morphological change, osteoblast presented a stellar shape and typical pseudopodes. SEM observations showed intercellular matrix containing fibers on HA-based porous aggregates. #</p><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":41404861,"attachmentType":"pdf","workUrl":"https://www.academia.edu/20498269/Growth_of_osteoblast_like_cells_on_porous_hydroxyapatite_ceramics_an_in_vitro_study"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":41404861,"attachmentType":"pdf","workUrl":"https://www.academia.edu/20498269/Growth_of_osteoblast_like_cells_on_porous_hydroxyapatite_ceramics_an_in_vitro_study"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div></div><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-doc_id="41404861" data-landing_url="https://www.academia.edu/20498269/Growth_of_osteoblast_like_cells_on_porous_hydroxyapatite_ceramics_an_in_vitro_study" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="18950721" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/18950721/Effect_of_hydroxyapatite_porosity_on_growth_and_differentiation_of_human_osteoblast_like_cells">Effect of hydroxyapatite porosity on growth and differentiation of human osteoblast-like cells</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="39083208" href="https://independent.academia.edu/PagnottaA">A. Pagnotta</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of materials …, 2002</p><p class="ds-related-work--abstract ds2-5-body-sm">To study whether hydroxyapatite (HA) porosity can influence its osteoconductive properties, cell adhesion, proliferation and differentiation were compared in human osteoblast-like cells grown on HA disks of different porosity (A= 20%, B= 40%, C= 60%). Human osteoblast-...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Effect of hydroxyapatite porosity on growth and differentiation of human osteoblast-like cells","attachmentId":42125089,"attachmentType":"pdf","work_url":"https://www.academia.edu/18950721/Effect_of_hydroxyapatite_porosity_on_growth_and_differentiation_of_human_osteoblast_like_cells","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/18950721/Effect_of_hydroxyapatite_porosity_on_growth_and_differentiation_of_human_osteoblast_like_cells"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="19446777" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/19446777/An_in_vitro_evaluation_of_coralline_porous_hydroxyapatite_as_a_scaffold_for_osteoblast_growth">An in-vitro evaluation of coralline porous hydroxyapatite as a scaffold for osteoblast growth</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="39721777" href="https://independent.academia.edu/EdwinShors">Edwin Shors</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Clinical Materials, 1994</p><p class="ds-related-work--abstract ds2-5-body-sm">The purpose of this study was to determine the potential of coralline calcium phosphate ceramics to support osteoblast growth for a proposed boneceramic composite for skeletal tissue repair. The goal was the development of a matrix with both osteogenic and osteoconductive properties, as compared to ceramic alone, which is solely osteoconductive. MC3T3-El osteoblast-like cells were seeded onto sintered and non-sintered porous coralline hydroxyapatite (HA), and onto non-porous hydroxyapatite discs. These in-vitro studies demonstrated that coralline HA supported the growth of osteoblast-like cells. Porous discs supported higher numbers of cells than non-porous discs. Sintering encouraged cell growth, with higher numbers of cells adhered to sintered porous HA discs by day seven. The results suggest that HA can provide a support for osteoblast cells as part of a matrix which may prove to be osteogenic in vivo and may, accordingly, enhance the bone repair process.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"An in-vitro evaluation of coralline porous hydroxyapatite as a scaffold for osteoblast growth","attachmentId":40624998,"attachmentType":"pdf","work_url":"https://www.academia.edu/19446777/An_in_vitro_evaluation_of_coralline_porous_hydroxyapatite_as_a_scaffold_for_osteoblast_growth","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/19446777/An_in_vitro_evaluation_of_coralline_porous_hydroxyapatite_as_a_scaffold_for_osteoblast_growth"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="3988364" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/3988364/Porous_hydroxyapatite_for_artificial_bone_applications">Porous hydroxyapatite for artificial bone applications</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="3364553" href="https://malaya.academia.edu/SRamesh">S. Ramesh</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Science and Technology of Advanced Materials, 2007</p><p class="ds-related-work--abstract ds2-5-body-sm">Hydroxyapatite (HA) has been used clinically for many years. It has good biocompatibility in bone contact as its chemical composition is similar to that of bone material. Porous HA ceramics have found enormous use in biomedical applications including bone tissue regeneration, cell proliferation, and drug delivery. In bone tissue engineering it has been applied as filling material for bone defects and augmentation, artificial bone graft material, and prosthesis revision surgery. Its high surface area leads to excellent osteoconductivity and resorbability providing fast bone ingrowth. Porous HA can be produced by a number of methods including conversion of natural bones, ceramic foaming technique, polymeric sponge method, gel casting of foams, starch consolidation, microwave processing, slip casting, and electrophoretic deposition technique. Some of these methods have been combined to fabricate porous HA with improved properties. These combination methods have yielded some promising results. This paper discusses briefly fundamental aspects of porous HA for artificial bone applications as well as various techniques used to prepare porous HA. Some of our recent results on development of porous HA will be presented as well. http://www.sciencedirect.com/science/article/pii/S1468699606002348</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Porous hydroxyapatite for artificial bone applications","attachmentId":31542499,"attachmentType":"pdf","work_url":"https://www.academia.edu/3988364/Porous_hydroxyapatite_for_artificial_bone_applications","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/3988364/Porous_hydroxyapatite_for_artificial_bone_applications"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="93281749" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/93281749/Interconnected_porous_hydroxyapatite_ceramics_for_bone_tissue_engineering">Interconnected porous hydroxyapatite ceramics for bone tissue engineering</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="96759932" href="https://osaka-u.academia.edu/TsuyoshiMurase">Tsuyoshi Murase</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of The Royal Society Interface, 2008</p><p class="ds-related-work--abstract ds2-5-body-sm">Several porous calcium hydroxyapatite (HA) ceramics have been used clinically as bone substitutes, but most of them possessed few interpore connections, resulting in pathological fracture probably due to poor bone formation within the substitute. We recently developed a fully interconnected porous HA ceramic (IP-CHA) by adopting the ‘foam-gel’ technique. The IP-CHA had a three-dimensional structure with spherical pores of uniform size (average 150 μm, porosity 75%), which were interconnected by window-like holes (average diameter 40 μm), and also demonstrated adequate compression strength (10–12 MPa). In animal experiments, the IP-CHA showed superior osteoconduction, with the majority of pores filled with newly formed bone. The interconnected porous structure facilitates bone tissue engineering by allowing the introduction of mesenchymal cells, osteotropic agents such as bone morphogenetic protein or vasculature into the pores. Clinically, we have applied the IP-CHA to treat various...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Interconnected porous hydroxyapatite ceramics for bone tissue engineering","attachmentId":96061657,"attachmentType":"pdf","work_url":"https://www.academia.edu/93281749/Interconnected_porous_hydroxyapatite_ceramics_for_bone_tissue_engineering","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/93281749/Interconnected_porous_hydroxyapatite_ceramics_for_bone_tissue_engineering"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="100889815" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/100889815/Fabrication_of_nanostructured_hydroxyapatite_and_analysis_of_human_osteoblastic_cellular_response">Fabrication of nanostructured hydroxyapatite and analysis of human osteoblastic cellular response</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="267493549" href="https://independent.academia.edu/guoxingyuan">xingyuan guo</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Biomedical Materials Research Part A, 2007</p><p class="ds-related-work--abstract ds2-5-body-sm">Nano-sized hydroxyapatite (HA) powders were produced by a hydrothermal method and a precipitation method. Spark plasma sintering (SPS) was used to fabricate nanostructured HA (NHA) using nano-sized HA powders as a precursor. Conventional sintering was employed to produce microstructured HA (MHA). Characteristics of HA powders and HA bulk ceramics after sintering were investigated by XRD, FTIR, SEM, TEM, particle size distribution, and AFM. Dense compacts consisting of equiaxed grains with an average grain size of *100 nm were obtained by SPS. Human osteoblasts were cultured on both NHA and MHA and cell attachment, proliferation, and mineralization were evaluated. After 90 min incubation, the cell density on NHA surface was significantly higher than that of MHA and glass control, whereas average cell area of a spread cell was significantly lower on NHA surface compared to MHA and glass control after 4 h incubation. Matrix mineralization was determined after 7 and 14 days incubation by using alizarin red assay combined with cetylpyridinium chloride extraction. NHA shows significant enhancement (p < 0.05) in mineralization compared to MHA. Results from this study suggest that NHA may be a much better candidate for clinical use in terms of bioactivity.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Fabrication of nanostructured hydroxyapatite and analysis of human osteoblastic cellular response","attachmentId":101583129,"attachmentType":"pdf","work_url":"https://www.academia.edu/100889815/Fabrication_of_nanostructured_hydroxyapatite_and_analysis_of_human_osteoblastic_cellular_response","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/100889815/Fabrication_of_nanostructured_hydroxyapatite_and_analysis_of_human_osteoblastic_cellular_response"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="122199744" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/122199744/Proliferation_and_Differentiation_of_Cultured_MC3T3_E1_Osteoblasts_on_Surface_layer_Modified_Hydroxyapatite_Ceramic_with_Acid_and_Heat_Treatments">Proliferation and Differentiation of Cultured MC3T3-E1 Osteoblasts on Surface-layer Modified Hydroxyapatite Ceramic with Acid and Heat Treatments</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="148396139" href="https://independent.academia.edu/HideyukiTAKANO1">Hideyuki TAKANO</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Dental Materials Journal, 2005</p><p class="ds-related-work--abstract ds2-5-body-sm">Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and a-tricalcium phosphate (a-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-a-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial, mid-stage, and late-stage osteoblastic differentiation. Basically, HAP-a-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP-a-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP-a-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP-a-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Proliferation and Differentiation of Cultured MC3T3-E1 Osteoblasts on Surface-layer Modified Hydroxyapatite Ceramic with Acid and Heat Treatments","attachmentId":116915160,"attachmentType":"pdf","work_url":"https://www.academia.edu/122199744/Proliferation_and_Differentiation_of_Cultured_MC3T3_E1_Osteoblasts_on_Surface_layer_Modified_Hydroxyapatite_Ceramic_with_Acid_and_Heat_Treatments","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/122199744/Proliferation_and_Differentiation_of_Cultured_MC3T3_E1_Osteoblasts_on_Surface_layer_Modified_Hydroxyapatite_Ceramic_with_Acid_and_Heat_Treatments"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="7443328" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/7443328/Osteogenesis_Depending_on_Geometry_of_Porous_Hydroxyapatite_Scaffolds">Osteogenesis Depending on Geometry of Porous Hydroxyapatite Scaffolds</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="13236409" href="https://osaka-dent.academia.edu/YasunoriShimomura">Yasunori Shimomura</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Calcified Tissue International, 2008</p><p class="ds-related-work--abstract ds2-5-body-sm">The effect of the configuration of porous cylindrical hydroxyapatite (HA) scaffold and laminin preparation of the scaffold on bone formation was estimated. HA scaffolds with a hollow center of 2 or 4 mm in diameter and those without a hollow center were used. The scaffolds were immersed in laminin solution or in culture medium. Bone marrow cells were obtained from the femora of male Fischer 344 rats. Cell suspension was prepared at 1 × 106 cells/mL density. The cells were seeded into HA scaffolds. Each scaffold was implanted in the dorsal subcutis of rats for 4 weeks. Bone formation in scaffolds was observed histologically. The quantity of osteocalcin was measured immunochemically. Many pores containing bone were identified in the laminin-immersed HA scaffold with a hollow center measuring 4 mm in diameter than those without and those with a hollow center measuring 2 mm in diameter. A greater quantity of osteocalcin was detected in the HA scaffold with immersion in laminin than in that without immersion in laminin. However, the results of the immunochemical assay for osteocalcin showed that a hollow center in the scaffold did not contribute to bone formation compared to scaffolds without a hollow center. It is considered that laminin may act as an adhesive for effective cell attachment to the walls of the pores in an HA scaffold.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Osteogenesis Depending on Geometry of Porous Hydroxyapatite Scaffolds","attachmentId":48472534,"attachmentType":"pdf","work_url":"https://www.academia.edu/7443328/Osteogenesis_Depending_on_Geometry_of_Porous_Hydroxyapatite_Scaffolds","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/7443328/Osteogenesis_Depending_on_Geometry_of_Porous_Hydroxyapatite_Scaffolds"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="29882031" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/29882031/Influence_of_hydroxyapatite_granule_size_porosity_and_crystallinity_on_tissue_reaction_in_vivo_Part_A_synthesis_characterization_of_the_materials_and_SEM_analysis">Inﬂuence of hydroxyapatite granule size, porosity, and crystallinity on tissue reaction in vivo. Part A: synthesis, characterization of the materials, and SEM analysis</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="52857278" href="https://umh-es.academia.edu/PatriciaMaz%C3%B3n">Patricia Mazón</a><span>, </span><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="51879046" href="https://umh-es.academia.edu/PiedaddeAzaMoya">Piedad N De Aza</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2016</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Inﬂuence of hydroxyapatite granule size, porosity, and crystallinity on tissue reaction in vivo. Part A: synthesis, characterization of the materials, and SEM analysis","attachmentId":50348333,"attachmentType":"pdf","work_url":"https://www.academia.edu/29882031/Influence_of_hydroxyapatite_granule_size_porosity_and_crystallinity_on_tissue_reaction_in_vivo_Part_A_synthesis_characterization_of_the_materials_and_SEM_analysis","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/29882031/Influence_of_hydroxyapatite_granule_size_porosity_and_crystallinity_on_tissue_reaction_in_vivo_Part_A_synthesis_characterization_of_the_materials_and_SEM_analysis"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="28098111" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/28098111/MC3T3_E1_osteoblast_attachment_and_proliferation_on_porous_hydroxyapatite_scaffolds_fabricated_with_nanophase_powder">MC3T3-E1 osteoblast attachment and proliferation on porous hydroxyapatite scaffolds fabricated with nanophase powder</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="52664488" href="https://independent.academia.edu/MelissaBaumann">Melissa Baumann</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Nanomedicine, 2006</p><p class="ds-related-work--abstract ds2-5-body-sm">Porous bone tissue engineering scaffolds were fabricated using both nano hydroxyapatite (nano HA) powder (20 nm average particle size) and micro HA powder (10 µm average particle size), resulting in sintered scaffolds of 59 vol% porosity and 8.6±1.9 µm average grain size and 72 vol% porosity and 588±55 nm average grain size, respectively. Scanning electron microscopy was used to measure both the grain size and pore size. MC3T3-E1 osteoblast (OB) attachment and proliferation on both nano HA and micro HA porous scaffolds were quantified. As expected, OB cell number was greater on nano HA scaffolds compared with similarly processed micro HA scaffolds 5 days after seeding, while OB attachment did not appear greater on the nano HA scaffolds (p<0.05).</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"MC3T3-E1 osteoblast attachment and proliferation on porous hydroxyapatite scaffolds fabricated with nanophase powder","attachmentId":48412941,"attachmentType":"pdf","work_url":"https://www.academia.edu/28098111/MC3T3_E1_osteoblast_attachment_and_proliferation_on_porous_hydroxyapatite_scaffolds_fabricated_with_nanophase_powder","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/28098111/MC3T3_E1_osteoblast_attachment_and_proliferation_on_porous_hydroxyapatite_scaffolds_fabricated_with_nanophase_powder"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="54611061" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/54611061/Engineering_new_bone_tissuein_vitro_on_highly_porous_poly_hydroxyl_acids_hydroxyapatite_composite_scaffolds">Engineering new bone tissuein vitro on highly porous poly(?-hydroxyl acids)/hydroxyapatite composite scaffolds</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33046521" href="https://independent.academia.edu/PeterMa1">Peter Ma</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Biomedical Materials Research, 2001</p><p class="ds-related-work--abstract ds2-5-body-sm">Engineering new bone tissue with cells and a synthetic extracellular matrix (scaffolding) represents a new approach for the regeneration of mineralized tissues compared with the transplantation of bone (autografts or allografts). In the present work, highly porous poly(L-lactic acid) (PLLA) and PLLA/hydroxyapatite (HAP) composite scaffolds were prepared with a thermally induced phase separation technique. The scaffolds were seeded with osteoblastic cells and cultured in vitro. In the pure PLLA scaffolds, the osteoblasts attached primarily on the outer surface of the polymer. In contrast, the osteoblasts penetrated deep into the PLLA/HAP scaffolds and were uniformly distributed. The osteoblast survival percentage in the PLLA/HAP scaffolds was superior to that in the PLLA scaffolds. The osteoblasts proliferated in both types of the scaffolds, but the cell number was always higher in the PLLA/HAP composite scaffolds during 6 weeks of in vitro cultivation. Bone-specific markers (mRNAs encoding bone sialoprotein and osteocalcin) were expressed more abundantly in the PLLA/HAP composite scaffolds than in the PLLA scaffolds. The new tissue increased continuously in the PLLA/HAP composite scaffolds, whereas new tissue formed only near the surface of pure PLLA scaffolds. These results demonstrate that HAP imparts osteoconductivity and the highly porous PLLA/ HAP composite scaffolds are superior to pure PLLA scaffolds for bone tissue engineering.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Engineering new bone tissuein vitro on highly porous poly(?-hydroxyl acids)/hydroxyapatite composite scaffolds","attachmentId":70893429,"attachmentType":"pdf","work_url":"https://www.academia.edu/54611061/Engineering_new_bone_tissuein_vitro_on_highly_porous_poly_hydroxyl_acids_hydroxyapatite_composite_scaffolds","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/54611061/Engineering_new_bone_tissuein_vitro_on_highly_porous_poly_hydroxyl_acids_hydroxyapatite_composite_scaffolds"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":41404861,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":41404861,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_41404861" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="66940070" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/66940070/A_triphasic_ceramic_coated_porous_hydroxyapatite_for_tissue_engineering_application">A triphasic ceramic-coated porous hydroxyapatite for tissue engineering application</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="190451528" href="https://independent.academia.edu/JohnAsabre">Asabre John</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Acta Biomaterialia, 2008</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"A triphasic ceramic-coated porous hydroxyapatite for tissue engineering application","attachmentId":77945866,"attachmentType":"pdf","work_url":"https://www.academia.edu/66940070/A_triphasic_ceramic_coated_porous_hydroxyapatite_for_tissue_engineering_application","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/66940070/A_triphasic_ceramic_coated_porous_hydroxyapatite_for_tissue_engineering_application"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="1" data-entity-id="61563776" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/61563776/Comparison_of_osteoblast_responses_to_hydroxyapatite_and_hydroxyapatite_soluble_calcium_phosphate_composites">Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="39174299" href="https://independent.academia.edu/TakayoshiNakano">Takayoshi Nakano</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Biomedical Materials Research Part A, 2005</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Comparison of osteoblast responses to hydroxyapatite and hydroxyapatite/soluble calcium phosphate composites","attachmentId":74559177,"attachmentType":"pdf","work_url":"https://www.academia.edu/61563776/Comparison_of_osteoblast_responses_to_hydroxyapatite_and_hydroxyapatite_soluble_calcium_phosphate_composites","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/61563776/Comparison_of_osteoblast_responses_to_hydroxyapatite_and_hydroxyapatite_soluble_calcium_phosphate_composites"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="2" data-entity-id="13690468" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/13690468/Porous_polymer_hydroxyapatite_scaffolds_characterization_and_biocompatibility_investigations">Porous polymer/hydroxyapatite scaffolds: characterization and biocompatibility investigations</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="52861099" href="https://independent.academia.edu/TimothyDouglas3">Timothy Douglas</a><span>, </span><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32829651" href="https://agh.academia.edu/ElzbietaPamula">Elzbieta Pamula</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Materials Science: Materials in Medicine, 2009</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Porous polymer/hydroxyapatite scaffolds: characterization and biocompatibility investigations","attachmentId":45054675,"attachmentType":"pdf","work_url":"https://www.academia.edu/13690468/Porous_polymer_hydroxyapatite_scaffolds_characterization_and_biocompatibility_investigations","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/13690468/Porous_polymer_hydroxyapatite_scaffolds_characterization_and_biocompatibility_investigations"><span class="ds2-5-text-link__content">View PDF</span><span 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