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nucleotide polymorphism</title> <meta name="description" content="Search results for: single nucleotide polymorphism"> <meta name="keywords" content="single nucleotide polymorphism"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="single nucleotide polymorphism"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4868</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: single nucleotide polymorphism</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4658</span> The Gradient Complex Protective Coatings for Single Crystal Nickel Alloys</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Evgeniya%20Popova">Evgeniya Popova</a>, <a href="https://publications.waset.org/abstracts/search?q=Vladimir%20Lesnikov"> Vladimir Lesnikov</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikolay%20Popov"> Nikolay Popov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> High yield complex coatings have been designed for thermally stressed cooled HP turbine blades from single crystal alloys ZHS32-VI-VI and ZHS36 with crystallographic orientation [001]. These coatings provide long-term protection of single crystal blades during operation. The three-layer coatings were prepared as follows: the diffusion barrier layer formation on the alloy surface, the subsequent deposition of the condensed bilayer coatings consisting of an inner layer based on Ni-Cr-Al-Y systems and an outer layer based on the alloyed β-phase. The structure, phase composition of complex coatings and reaction zone interaction with the single-crystal alloys ZHS32-VI and ZHS36-VI were investigated using scanning electron microscope (SEM). The effect of complex protective coatings on the properties of heat-resistant nickel alloys was studied. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=single%20crystal%20nickel%20alloys" title="single crystal nickel alloys">single crystal nickel alloys</a>, <a href="https://publications.waset.org/abstracts/search?q=complex%20heat-resistant%20coatings" title=" complex heat-resistant coatings"> complex heat-resistant coatings</a>, <a href="https://publications.waset.org/abstracts/search?q=structure" title=" structure"> structure</a>, <a href="https://publications.waset.org/abstracts/search?q=phase%20composition" title=" phase composition"> phase composition</a>, <a href="https://publications.waset.org/abstracts/search?q=properties" title=" properties"> properties</a> </p> <a href="https://publications.waset.org/abstracts/63315/the-gradient-complex-protective-coatings-for-single-crystal-nickel-alloys" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63315.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">418</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4657</span> The Contribution of Genetic Polymorphisms of Tumor Necrosis Factor Alpha and Vascular Endothelial Growth Factor into the Unfavorable Clinical Course of Ulcerative Colitis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Y.%20I.%20Tretyakova">Y. I. Tretyakova</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20G.%20Shulkina"> S. G. Shulkina</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Y.%20Kravtsova"> T. Y. Kravtsova</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A.%20Antipova"> A. A. Antipova</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Y.%20Kolomeets"> N. Y. Kolomeets</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The research aimed to assess the functional significance of tumor necrosis factor-alpha (TNF-α) gene polymorphism at the -308G/A (rs1800629) region and vascular endothelial growth factor A (VEGFA) gene polymorphism at the -634G/C (rs 2010963) region in the development of ulcerative colitis (UC), focusing on patients from the Perm region, Russia. We examined 70 UC patients and 50 healthy donors during the active phase of the disease. Our focus was on TNF-α and VEGF concentration in the blood serum, as well as TNF-α and VEGFA gene polymorphisms at the -308G/А and -634G/C regions, respectively. We found that TNF-α and VEGF levels were significantly higher in patients with severe UC and high endoscopic activity compared to those with milder forms of the disease and low endoscopic activity. These tests could serve as additional non-invasive markers for assessing mucosal damage in the large intestine of UC patients. The frequency of allele variations in the TNF-α gene -308G/A (rs1800629) revealed a significantly higher occurrence of the unfavorable homozygote AA in UC patients compared to donors. Additionally, the major allele G and the allele pair GG were more frequent in patients with mild to moderate disease and 1-2 degree of endoscopic activity than in those with severe UC and 3-4 degree of endoscopic activity (χ2=14.19; p=0.000). We also observed a mutant allele A and the unfavorable homozygote AA associated with severe progressive UC. The occurrence of the mutant allele increased the risk of severe UC by 5 times (OR 5.03; CI 12.07-12.21). We did not find any significant differences in the frequency of the CC homozygote (χ2=1.02; p=0.6; OR=1.32) and the mutant allele C of the VEGFA gene -634G/C (rs 2010963) (χ2=0.01; p=0.913; OR=0.97) between groups of UC patients and healthy individuals. However, we detected that the mutant allele C and the unfavorable homozygote CC of the VEGFA gene were associated with more severe endoscopic changes in the colonic mucosa of UC patients (χ2=25,76; р=0,000; OR=0,15). The presence of the mutant allele increased the risk of severe UC by 6 times (OR 6,78; CI 3,13–14,7). We found a direct correlation between TNF-α and VEGFA gene polymorphisms, increased production of the same factors, disease severity, and endoscopic activity (р=0.000). Therefore, the presence of the mutant allele A and homozygote AA of the TNF-α gene at the -308G/A region and the mutant allele C and homozygote CC of the VEGFA gene at the -634G/C region are associated with risks related to an unfavorable clinical course of UC, frequent recurrences, and rapid progression. These findings should be considered when making prognoses regarding the clinical course of the disease and selecting treatment strategies. The presence of the homozygote AA in the TNF-α gene (rs1800629) is considered a sign of genetic predisposition to UC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gene%20polymorphism" title="gene polymorphism">gene polymorphism</a>, <a href="https://publications.waset.org/abstracts/search?q=TNF-%CE%B1" title=" TNF-α"> TNF-α</a>, <a href="https://publications.waset.org/abstracts/search?q=ulcerative%20colitis" title=" ulcerative colitis"> ulcerative colitis</a>, <a href="https://publications.waset.org/abstracts/search?q=VEGF" title=" VEGF"> VEGF</a> </p> <a href="https://publications.waset.org/abstracts/174671/the-contribution-of-genetic-polymorphisms-of-tumor-necrosis-factor-alpha-and-vascular-endothelial-growth-factor-into-the-unfavorable-clinical-course-of-ulcerative-colitis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/174671.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">74</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4656</span> Mediating Role of Burnout in Personality and Marital Satisfaction of Single and Dual Career Couples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sara%20Subhan">Sara Subhan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Married couples tend to experience various bio-psycho-social issues that may eventually impact the quality of their marital relationship and mental wellbeing. This study aimed to find out the comparison between the single and dual-career couples’ personality, burnout and marital satisfaction. For that purpose Big Five Inventory, Couple Satisfaction Inventory, and Maslach Burnout Inventory-General Survey was used to measure the relationship between variables. The main study was carried out on 200 samples of single and dual-earner couples with the age range of 23-52 (mean= 34.58; standard deviation= 6.51) by using a purposive sampling strategy. The results showed that burnout tendencies like exhaustion, cynicism and professional efficacy are playing a mediation role between the personality and marital satisfaction of both single and dual career couples. Also, the results revealed that dual-career couples are more likely to have marital satisfaction as compared to single career couples. The results were further discussed in the light of its implications in its cultural context and counseling areas. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dual%20career%20couples" title="dual career couples">dual career couples</a>, <a href="https://publications.waset.org/abstracts/search?q=marital%20satisfaction" title=" marital satisfaction"> marital satisfaction</a>, <a href="https://publications.waset.org/abstracts/search?q=burnout%20tendencies" title=" burnout tendencies"> burnout tendencies</a>, <a href="https://publications.waset.org/abstracts/search?q=personality" title=" personality"> personality</a> </p> <a href="https://publications.waset.org/abstracts/104310/mediating-role-of-burnout-in-personality-and-marital-satisfaction-of-single-and-dual-career-couples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104310.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">173</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4655</span> Genetic Analysis of CYP11A1 Gene with Polycystic Ovary Syndrome from North India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ratneev%20Kaur">Ratneev Kaur</a>, <a href="https://publications.waset.org/abstracts/search?q=Tajinder%20Kaur"> Tajinder Kaur</a>, <a href="https://publications.waset.org/abstracts/search?q=Anupam%20Kaur"> Anupam Kaur</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Polycystic Ovary Syndrome (PCOS) is a heterogenous disorder of endocrine system among women of reproductive age. PCOS is characterized by hyperandrogenism, anovulation, polycystic ovaries, hirsutism, obesity, and hyperinsulinemia. Several pathways are implicated in its etiology including the metabolic pathway of steroid hormone synthesis regulatory pathways. PCOS is an androgen excess disorder, genes operating in steroidogenesis may alter pathogenesis of PCOS. The cytochrome P450scc is a cholesterol side chain cleavage enzyme coded by CYP11A1 gene and catalyzes conversion of cholesterol to pregnenolone, the initial and rate-limiting step in steroid hormone synthesis. It is postulated that polymorphisms in this gene may play an important role in the regulation of CYP11A1 expression and leading to increased or decreased androgen production. The present study will be the first study from north India to best of our knowledge, to analyse the association of CYP11A1 (rs11632698) polymorphism in women suffering from PCOS. Methodology: The present study was approved by ethical committee of Guru Nanak Dev University in consistent with declaration of Helsinki. A total of 300 samples (150 PCOS cases and 150 controls) were recruited from Hartej hospital, for the present study. Venous blood sample (3ml) was withdrawn from women diagnosed with PCOS by doctor, according to Rotterdam 2003 criteria and from healthy age matched controls only after informed consent and detailed filled proforma. For molecular genetics analysis, blood was stored in EDTA vials. After DNA isolation by organic method, PCR-RFLP approach was used for genotyping and association analysis of rs11632698 polymorphism. Statistical analysis was done to check for significance of selected polymorphism with PCOS. Results: In 150 PCOS cases, the frequency of AA, AG and GG genotype was found to be 48%, 35%, and 13% compared to 62%, 27% and 8% in 150 controls. The major allele (A) and minor allele (G) frequency was 68% and 32% in cases and 78% and 22% in controls. Minor allele frequency was higher in cases as compared to controls, as well as the distribution of genotype was observed to be statistically significant (ᵡ²=6.525, p=0.038). Odds ratio in dominant, co-dominant and recessive models observed was 1.81 (p=0.013), 1.54 (p=0.012) and 1.77 (p=0.132) respectively. Conclusion: The present study showed statistically significant association of rs11632698 with PCOS (p=0.038) in North Indian women. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=polycystic%20ovary%20syndrome" title="polycystic ovary syndrome">polycystic ovary syndrome</a>, <a href="https://publications.waset.org/abstracts/search?q=CYP11A1" title=" CYP11A1"> CYP11A1</a>, <a href="https://publications.waset.org/abstracts/search?q=rs11632698" title=" rs11632698"> rs11632698</a>, <a href="https://publications.waset.org/abstracts/search?q=hyperandrogenism" title=" hyperandrogenism"> hyperandrogenism</a> </p> <a href="https://publications.waset.org/abstracts/99786/genetic-analysis-of-cyp11a1-gene-with-polycystic-ovary-syndrome-from-north-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99786.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">142</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4654</span> Obesity, Leptin Levels and Leptin Receptor Gene Polymorphisms in Afro-Caribbean Subjects</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lydia%20Foucan">Lydia Foucan</a>, <a href="https://publications.waset.org/abstracts/search?q=Christine%20Rambhojan"> Christine Rambhojan</a>, <a href="https://publications.waset.org/abstracts/search?q=Rachel%20Billy"> Rachel Billy</a>, <a href="https://publications.waset.org/abstracts/search?q=Christophe%20Armand"> Christophe Armand</a>, <a href="https://publications.waset.org/abstracts/search?q=Carl-Thony%20Michel"> Carl-Thony Michel</a>, <a href="https://publications.waset.org/abstracts/search?q=Jean-Marc%20Lacorte"> Jean-Marc Lacorte</a>, <a href="https://publications.waset.org/abstracts/search?q=Laurent%20Larifla"> Laurent Larifla</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leptin, an adipocyte-derived hormone, modulates insulin secretion and action via the leptin receptor (LEPR) that is expressed in pancreatic beta cells, adipose tissue, and muscle. Several polymorphisms have been described in the human LEPR gene including p.K109R (rs1137100), p.Q223R (rs1137101) and p.K656N (rs1805094) polymorphisms. The role of these polymorphisms is not yet studied in Guadeloupian population. Our aim was to explore the association of LEPR polymorphisms (K109R, Q223R and K656N) with leptin levels and obesity in non-diabetic Afro-Caribbean subjects. Genotypic analysis of the three polymorphisms was performed in 425 subjects using TaqMan and KASPar Assays. Serum leptin was measured with ELISA kits Biovendor® (RD191001100). Logistic regressions were used for assessment of statistical associations. Mean age was 47.6 ± 12.7 years. Among the participants, 238 (56 %) were women, 124 (30%) were obese and 155 (36.5%) had abdominal obesity. Carriers of LEPR K656N rs1805094 rare allele had significant higher frequencies of obesity (P = 0.007), abdominal obesity (P = 0.004) and metabolic syndrome (P = 0.021) but mean leptin level was not significantly different between both groups (P = 0.075). Odds ratios, adjusted for age and sex associated with presence of rs1805094 rare allele were 1.8 (1.1-2.9), P = 0.012 for obesity, 2.0 (1.2-3.3), P = 0.008 for abdominal obesity and 1.8 (1.1-3.0), P = 0.031 for MetS. No significant association was found with K109R, Q223R. These findings suggest that the K656N polymorphism (but not the K109R or Q223R polymorphism) of LEPR is associated with obesity, abdominal obesity and metabolic syndrome in this Afro-Caribbean non-diabetic population. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Afro-Caribbean" title="Afro-Caribbean">Afro-Caribbean</a>, <a href="https://publications.waset.org/abstracts/search?q=leptin%20levels" title=" leptin levels"> leptin levels</a>, <a href="https://publications.waset.org/abstracts/search?q=leptin%20receptor%20gene%20polymorphisms" title=" leptin receptor gene polymorphisms"> leptin receptor gene polymorphisms</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a> </p> <a href="https://publications.waset.org/abstracts/86912/obesity-leptin-levels-and-leptin-receptor-gene-polymorphisms-in-afro-caribbean-subjects" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86912.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">377</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4653</span> Polyphenol-Rich Aronia Melanocarpa Juice Consumption and Line-1 Dna Methylation in a Cohort at Cardiovascular Risk</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ljiljana%20Stojkovi%C4%87">Ljiljana Stojković</a>, <a href="https://publications.waset.org/abstracts/search?q=Manja%20Zec"> Manja Zec</a>, <a href="https://publications.waset.org/abstracts/search?q=Maja%20Zivkovic"> Maja Zivkovic</a>, <a href="https://publications.waset.org/abstracts/search?q=Maja%20Bundalo"> Maja Bundalo</a>, <a href="https://publications.waset.org/abstracts/search?q=Marija%20Glibeti%C4%87"> Marija Glibetić</a>, <a href="https://publications.waset.org/abstracts/search?q=Dragan%20Alavanti%C4%87"> Dragan Alavantić</a>, <a href="https://publications.waset.org/abstracts/search?q=Aleksandra%20Stankovic"> Aleksandra Stankovic</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cardiovascular disease (CVD) is associated with alterations in DNA methylation, the latter modulated by dietary polyphenols. The present pilot study (part of the original clinical study registered as NCT02800967 at www.clinicaltrials.gov) aimed to investigate the impact of 4-week daily consumption of polyphenol-rich Aronia melanocarpa juice on Long Interspersed Nucleotide Element-1 (LINE-1) methylation in peripheral blood leukocytes, in subjects (n=34, age of 41.1±6.6 years) at moderate CVD risk, including an increased body mass index, central obesity, high normal blood pressure and/or dyslipidemia. The goal was also to examine whether factors known to affect DNA methylation, such as folate intake levels, MTHFR C677T gene variant, as well as the anthropometric and metabolic parameters, modulated the LINE-1 methylation levels upon consumption of polyphenol-rich Aronia juice. The experimental analysis of LINE-1 methylation was done by the MethyLight method. MTHFR C677T genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism method. Folate intake was assessed by processing the data from the food frequency questionnaire and repeated 24-hour dietary recalls. Serum lipid profile was determined by using Roche Diagnostics kits. The statistical analyses were performed using the Statistica software package. In women, after vs. before the treatment period, a significant decrease in LINE-1 methylation levels was observed (97.54±1.50% vs. 98.39±0.86%, respectively; P=0.01). The change (after vs. before treatment) in LINE-1 methylation correlated directly with MTHFR 677T allele presence, average daily folate intake and the change in serum low-density lipoprotein cholesterol, while inversely with the change in serum triacylglycerols (R=0.72, R2=0.52, adjusted R2=0.36, P=0.03). The current results imply potential cardioprotective effects of habitual polyphenol-rich Aronia juice consumption achieved through the modifications of DNA methylation pattern in subjects at CVD risk, which should be further confirmed. Hence, the precision nutrition-driven modulations of DNA methylation may become targets for new approaches in the prevention and treatment of CVD. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aronia%20melanocarpa" title="Aronia melanocarpa">Aronia melanocarpa</a>, <a href="https://publications.waset.org/abstracts/search?q=cardiovascular%20risk" title=" cardiovascular risk"> cardiovascular risk</a>, <a href="https://publications.waset.org/abstracts/search?q=LINE-1" title=" LINE-1"> LINE-1</a>, <a href="https://publications.waset.org/abstracts/search?q=methylation" title=" methylation"> methylation</a>, <a href="https://publications.waset.org/abstracts/search?q=peripheral%20blood%20leukocytes" title=" peripheral blood leukocytes"> peripheral blood leukocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=polyphenol" title=" polyphenol"> polyphenol</a> </p> <a href="https://publications.waset.org/abstracts/131613/polyphenol-rich-aronia-melanocarpa-juice-consumption-and-line-1-dna-methylation-in-a-cohort-at-cardiovascular-risk" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/131613.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">195</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4652</span> Genetic Structure of Four Bovine Populations in the Philippines Using Microsatellites</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Peter%20James%20C.%20Icalia">Peter James C. Icalia</a>, <a href="https://publications.waset.org/abstracts/search?q=Agapita%20J.%20Salces"> Agapita J. Salces</a>, <a href="https://publications.waset.org/abstracts/search?q=Loida%20Valenzuela">Loida Valenzuela</a>, <a href="https://publications.waset.org/abstracts/search?q=Kangseok%20Seo"> Kangseok Seo</a>, <a href="https://publications.waset.org/abstracts/search?q=Geronima%20Ludan"> Geronima Ludan </a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study evaluated polymorphism of 11 microsatellite markers in four local genetic groups of cattle. Batanes cattle which has never been studied using microsatellites is evaluated for its genetic distance from the Ilocos cattle while Brahman and Holstein-Sahiwal are also included as there were insemination programs by the government using these two breeds. PCR products that were genotyped for each marker were analyzed using POPGENEv32. Results showed that 55% (Fst=0.5501) of the genetic variation is due to the differences between populations while the remaining 45% is due to individual variation. The Fst value also indicates that there were very great differences from population to population using the range proposed by Sewall and Wright. The constructed phylogenetic tree based on Nei’s genetic distance using the modified neighboor joining procedure of PHYLIPv3.5 showed the admixture of Brahman and Holstein-Sahiwal having them grouped in the same clade. Batanes and Ilocos cattle were grouped in a different cluster showing that they have descended from a single parental population. This would presumably address the claim that Batanes and Ilocos cattle are genetically distant from other groups and still exist despite the artificial insemination program of the government using Brahman and other imported breeds. The knowledge about the genetic structure of this population supports the development of conservation programs for the smallholder farmers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microsatellites" title="microsatellites">microsatellites</a>, <a href="https://publications.waset.org/abstracts/search?q=cattle" title=" cattle"> cattle</a>, <a href="https://publications.waset.org/abstracts/search?q=Philippines" title=" Philippines"> Philippines</a>, <a href="https://publications.waset.org/abstracts/search?q=populations" title=" populations"> populations</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20structure" title=" genetic structure"> genetic structure</a> </p> <a href="https://publications.waset.org/abstracts/12329/genetic-structure-of-four-bovine-populations-in-the-philippines-using-microsatellites" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12329.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">515</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4651</span> Wind Turbine Powered Car Uses 3 Single Big C-Section Blades</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Youssef">K. Youssef</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%87.%20H%C3%BCseyin"> Ç. Hüseyin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The blades of a wind turbine have the most important job of any wind turbine component; they must capture the wind and convert it into usable mechanical energy. The objective of this work is to determine the mechanical power of single big C-section of vertical wind turbine for wind car in a two-dimensional model. The wind car has a vertical axis with 3 single big C-section blades mounted at an angle of 120°. Moreover, the three single big C-section blades are directly connected to wheels by using various kinds of links. Gears are used to convert the wind energy to mechanical energy to overcome the load exercised on the main shaft under low speed. This work allowed a comparison of drag characteristics and the mechanical power between the single big C-section blades with the previous work on 3 C-section and 3 double C-section blades for wind car. As a result obtained from the flow chart the torque and power curves of each case study are illustrated and compared with each other. In particular, drag force and torque acting on each types of blade was taken at an airflow speed of 4 m/s, and an angular velocity of 13.056 rad/s. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=blade" title="blade">blade</a>, <a href="https://publications.waset.org/abstracts/search?q=vertical%20wind%20turbine" title=" vertical wind turbine"> vertical wind turbine</a>, <a href="https://publications.waset.org/abstracts/search?q=drag%20characteristics" title=" drag characteristics"> drag characteristics</a>, <a href="https://publications.waset.org/abstracts/search?q=mechanical%20power" title=" mechanical power"> mechanical power</a> </p> <a href="https://publications.waset.org/abstracts/16229/wind-turbine-powered-car-uses-3-single-big-c-section-blades" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16229.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">520</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4650</span> Metagenomics-Based Molecular Epidemiology of Viral Diseases</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vyacheslav%20Furtak">Vyacheslav Furtak</a>, <a href="https://publications.waset.org/abstracts/search?q=Merja%20Roivainen"> Merja Roivainen</a>, <a href="https://publications.waset.org/abstracts/search?q=Olga%20Mirochnichenko"> Olga Mirochnichenko</a>, <a href="https://publications.waset.org/abstracts/search?q=Majid%20Laassri"> Majid Laassri</a>, <a href="https://publications.waset.org/abstracts/search?q=Bella%20Bidzhieva"> Bella Bidzhieva</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatiana%20Zagorodnyaya"> Tatiana Zagorodnyaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Vladimir%20Chizhikov"> Vladimir Chizhikov</a>, <a href="https://publications.waset.org/abstracts/search?q=Konstantin%20Chumakov"> Konstantin Chumakov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Molecular epidemiology and environmental surveillance are parts of a rational strategy to control infectious diseases. They have been widely used in the worldwide campaign to eradicate poliomyelitis, which otherwise would be complicated by the inability to rapidly respond to outbreaks and determine sources of the infection. The conventional scheme involves isolation of viruses from patients and the environment, followed by their identification by nucleotide sequences analysis to determine phylogenetic relationships. This is a tedious and time-consuming process that yields definitive results when it may be too late to implement countermeasures. Because of the difficulty of high-throughput full-genome sequencing, most such studies are conducted by sequencing only capsid genes or their parts. Therefore the important information about the contribution of other parts of the genome and inter- and intra-species recombination to viral evolution is not captured. Here we propose a new approach based on the rapid concentration of sewage samples with tangential flow filtration followed by deep sequencing and reconstruction of nucleotide sequences of viruses present in the samples. The entire nucleic acids content of each sample is sequenced, thus preserving in digital format the complete spectrum of viruses. A set of rapid algorithms was developed to separate deep sequence reads into discrete populations corresponding to each virus and assemble them into full-length consensus contigs, as well as to generate a complete profile of sequence heterogeneities in each of them. This provides an effective approach to study molecular epidemiology and evolution of natural viral populations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=poliovirus" title="poliovirus">poliovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=eradication" title=" eradication"> eradication</a>, <a href="https://publications.waset.org/abstracts/search?q=environmental%20surveillance" title=" environmental surveillance"> environmental surveillance</a>, <a href="https://publications.waset.org/abstracts/search?q=laboratory%20diagnosis" title=" laboratory diagnosis"> laboratory diagnosis</a> </p> <a href="https://publications.waset.org/abstracts/37436/metagenomics-based-molecular-epidemiology-of-viral-diseases" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37436.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4649</span> Role of Estrogen Receptor-alpha in Mammary Carcinoma by Single Nucleotide Polymorphisms and Molecular Docking: An In-silico Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asif%20Bilal">Asif Bilal</a>, <a href="https://publications.waset.org/abstracts/search?q=Fouzia%20Tanvir"> Fouzia Tanvir</a>, <a href="https://publications.waset.org/abstracts/search?q=Sibtain%20Ahmad"> Sibtain Ahmad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Estrogen receptor alpha, also known as estrogen receptor-1, is highly involved in risk of mammary carcinoma. The objectives of this study were to identify non-synonymous SNPs of estrogen receptor and their association with breast cancer and to identify the chemotherapeutic responses of phytochemicals against it via in-silico study design. For this purpose, different online tools. to identify pathogenic SNPs the tools were SIFT, Polyphen, Polyphen-2, fuNTRp, SNAP2, for finding disease associated SNPs the tools SNP&GO, PhD-SNP, PredictSNP, MAPP, SNAP, MetaSNP, PANTHER, and to check protein stability Mu-Pro, I-Mutant, and CONSURF were used. Post-translational modifications (PTMs) were detected by Musitedeep, Protein secondary structure by SOPMA, protein to protein interaction by STRING, molecular docking by PyRx. Seven SNPs having rsIDs (rs760766066, rs779180038, rs956399300, rs773683317, rs397509428, rs755020320, and rs1131692059) showing mutations on I229T, R243C, Y246H, P336R, Q375H, R394S, and R394H, respectively found to be completely deleterious. The PTMs found were 96 times Glycosylation; 30 times Ubiquitination, a single time Acetylation; and no Hydroxylation and Phosphorylation were found. The protein secondary structure consisted of Alpha helix (Hh) is (28%), Extended strand (Ee) is (21%), Beta turn (Tt) is 7.89% and Random coil (Cc) is (44.11%). Protein-protein interaction analysis revealed that it has strong interaction with Myeloperoxidase, Xanthine dehydrogenase, carboxylesterase 1, Glutathione S-transferase Mu 1, and with estrogen receptors. For molecular docking we used Asiaticoside, Ilekudinuside, Robustoflavone, Irinoticane, Withanolides, and 9-amin0-5 as ligands that extract from phytochemicals and docked with this protein. We found that there was great interaction (from -8.6 to -9.7) of these ligands of phytochemicals at ESR1 wild and two mutants (I229T and R394S). It is concluded that these SNPs found in ESR1 are involved in breast cancer and given phytochemicals are highly helpful against breast cancer as chemotherapeutic agents. Further in vitro and in vivo analysis should be performed to conduct these interactions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title="breast cancer">breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=ESR1" title=" ESR1"> ESR1</a>, <a href="https://publications.waset.org/abstracts/search?q=phytochemicals" title=" phytochemicals"> phytochemicals</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20docking" title=" molecular docking"> molecular docking</a> </p> <a href="https://publications.waset.org/abstracts/175362/role-of-estrogen-receptor-alpha-in-mammary-carcinoma-by-single-nucleotide-polymorphisms-and-molecular-docking-an-in-silico-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175362.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">69</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4648</span> Molecular Characterization, Host Plant Resistance and Epidemiology of Bean Common Mosaic Virus Infecting Cowpea (Vigna unguiculata L. Walp)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=N.%20Manjunatha">N. Manjunatha</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20T.%20Rangswamy"> K. T. Rangswamy</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Nagaraju"> N. Nagaraju</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20A.%20Prameela"> H. A. Prameela</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Rudraswamy"> P. Rudraswamy</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Krishnareddy"> M. Krishnareddy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The identification of virus in cowpea especially potyviruses is confusing. Even though there are several studies on viruses causing diseases in cowpea, difficult to distinguish based on symptoms and serological detection. The differentiation of potyviruses considering as a constraint, the present study is initiated for molecular characterization, host plant resistance and epidemiology of the BCMV infecting cowpea. The etiological agent causing cowpea mosaic was identified as Bean Common Mosaic Virus (BCMV) on the basis of RT-PCR and electron microscopy. An approximately 750bp PCR product corresponding to coat protein (CP) region of the virus and the presence of long flexuous filamentous particles measuring about 952 nm in size typical to genus potyvirus were observed under electron microscope. The characterized virus isolate genome had 10054 nucleotides, excluding the 3’ terminal poly (A) tail. Comparison of polyprotein of the virus with other potyviruses showed similar genome organization with 9 cleavage sites resulted in 10 functional proteins. The pairwise sequence comparison of individual genes, P1 showed most divergent, but CP gene was less divergent at nucleotide and amino acid level. A phylogenetic tree constructed based on multiple sequence alignments of the polyprotein nucleotide and amino acid sequences of cowpea BCMV and potyviruses showed virus is closely related to BCMV-HB. Whereas, Soybean variant of china (KJ807806) and NL1 isolate (AY112735) showed 93.8 % (5’UTR) and 94.9 % (3’UTR) homology respectively with other BCMV isolates. This virus transmitted to different leguminous plant species and produced systemic symptoms under greenhouse conditions. Out of 100 cowpea genotypes screened, three genotypes viz., IC 8966, V 5 and IC 202806 showed immune reaction in both field and greenhouse conditions. Single marker analysis (SMA) was revealed out of 4 SSR markers linked to BCMV resistance, M135 marker explains 28.2 % of phenotypic variation (R2) and Polymorphic information content (PIC) value of these markers was ranged from 0.23 to 0.37. The correlation and regression analysis showed rainfall, and minimum temperature had significant negative impact and strong relationship with aphid population, whereas weak correlation was observed with disease incidence. Path coefficient analysis revealed most of the weather parameters exerted their indirect contributions to the aphid population and disease incidence except minimum temperature. This study helps to identify specific gaps in knowledge for researchers who may wish to further analyse the science behind complex interactions between vector-virus and host in relation to the environment. The resistant genotypes identified are could be effectively used in resistance breeding programme. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cowpea" title="cowpea">cowpea</a>, <a href="https://publications.waset.org/abstracts/search?q=epidemiology" title=" epidemiology"> epidemiology</a>, <a href="https://publications.waset.org/abstracts/search?q=genotypes" title=" genotypes"> genotypes</a>, <a href="https://publications.waset.org/abstracts/search?q=virus" title=" virus "> virus </a> </p> <a href="https://publications.waset.org/abstracts/70727/molecular-characterization-host-plant-resistance-and-epidemiology-of-bean-common-mosaic-virus-infecting-cowpea-vigna-unguiculata-l-walp" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/70727.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">236</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4647</span> Breast Cancer and BRCA Gene: A Study on Genetic and Environmental Interaction</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abhishikta%20Ghosh%20Roy">Abhishikta Ghosh Roy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Breast cancer is the most common malignancy among women globally, including India. Human breast cancer results from the genetic and environmental interaction. The present study attempts to understand the molecular heterogeneity of BRCA1 and BRCA2 genes, as well as to understand the association of various lifestyle and reproductive variables for the Breast Cancer risk. The study was conducted amongst 110 patients and 128 controls with total DNA sequencing of flanking and coding regions of BRCA1 BRCA2 genes that revealed ten Single Nucleotide Polymorphisms (SNPs) (6 novels). The controls selected for the study were age, sex and ethnic group matched. After written and informed consent biological samples were collected from the subjects. After detailed molecular analysis, significant (p < 0.005) molecular heterogeneity is revealed in terms of SNPs in BRCA1 (4 Exonic & 1 Intronic) and BRCA2 (2exonic and 3 Intronic) genes. The augmentation study investigated significant (p < 0.05) association with positive family history, early age at menarche, irregular menstrual periods, menopause, prolong contraceptive use, nulliparity, history of abortions, consumption of alcohol and smoking for breast cancer risk. To the best of authors knowledge, this study is the first of its kind, envisaged that the identification of the SNPs and modification of the lifestyle factors might aid to minimize the risk among the Bengalee Hindu females. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title="breast cancer">breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=BRCA" title=" BRCA"> BRCA</a>, <a href="https://publications.waset.org/abstracts/search?q=lifestyle" title=" lifestyle"> lifestyle</a>, <a href="https://publications.waset.org/abstracts/search?q=India" title=" India"> India</a> </p> <a href="https://publications.waset.org/abstracts/99371/breast-cancer-and-brca-gene-a-study-on-genetic-and-environmental-interaction" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99371.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">114</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4646</span> Angiotensin Converting Enzyme (ACE) and Angiotensinogen (AGT) Gene Variants in Pakistani Patients of Diabetes Mellitus and Diabetic Nephropathy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rozeena%20Shaikh">Rozeena Shaikh</a>, <a href="https://publications.waset.org/abstracts/search?q=Syed%20M%20Shahid"> Syed M Shahid</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamil%20Ahmad"> Jamil Ahmad</a>, <a href="https://publications.waset.org/abstracts/search?q=Qaisar%20Mansoor"> Qaisar Mansoor</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Ismail"> Muhammad Ismail</a>, <a href="https://publications.waset.org/abstracts/search?q=Abid%20Azhar"> Abid Azhar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Diabetes mellitus (DM) is a prevalent non-communicable disease worldwide. In most high-income countries as well as middle-income and low- income countries. DM is among the top causes of deaths. DM may lead to many vascular complications like hypertension, nephropathy, retinopathy, neuropathy, and foot. Diabetic nephropathy (DN) characterized by persistent albuminuria is a leading cause of end stage renal failure (ESRF). Pathogenesis of diabetic nephropathy is implicated by the polymorphisms in genes encoding the components of reninangiotensin- aldosteron system (RAAS) which include angiotensinogen (AGT), angiotensin-II receptor and particularly angiotensin converting enzyme (ACE) gene. Method: Study subjects include 110 control, 110 patients with DM without hypertension, 110 patients with DM with hypertension and 110 patients with DN. Blood samples were collected for Biochemical analysis and PCR and sequencing for the specific region of both genes. Results: The frequency of DD genotype and D allele of ACE (I/D) was significantly (p<0.05) high in DM normotensive, DM hypertensive and DN patients when compared to control. The ACE G2350A genotypes and allele frequencies were significantly different (p<0.05) in DM hypertensive patients as compared to control and DN, while no difference was observed between DM normotensive and DN when compared to control. The genotypes and alleles of AGT (M268T) polymorphism were significantly different (p<0.05) in DM normotensive, DM hypertensive and DN when compared to control. Conclusion: The DD genotype and D allele of ACE (I/D), GG genotype and G allele of ACE (G2350A) and the TT genotype and T allele of AGT (M268T) polymorphism have shown a significant difference in genotype and allele frequencies between controls and patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=genetic%20variations" title="genetic variations">genetic variations</a>, <a href="https://publications.waset.org/abstracts/search?q=ACE" title=" ACE"> ACE</a>, <a href="https://publications.waset.org/abstracts/search?q=AGT" title=" AGT"> AGT</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetes%20mellitus" title=" diabetes mellitus"> diabetes mellitus</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetic%20nephropathy" title=" diabetic nephropathy"> diabetic nephropathy</a>, <a href="https://publications.waset.org/abstracts/search?q=Pakistan" title=" Pakistan"> Pakistan</a> </p> <a href="https://publications.waset.org/abstracts/15166/angiotensin-converting-enzyme-ace-and-angiotensinogen-agt-gene-variants-in-pakistani-patients-of-diabetes-mellitus-and-diabetic-nephropathy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15166.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">392</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4645</span> Transient Voltage Distribution on the Single Phase Transmission Line under Short Circuit Fault Effect</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Kojah">A. Kojah</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Nacaro%C4%9Flu"> A. Nacaroğlu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Single phase transmission lines are used to transfer data or energy between two users. Transient conditions such as switching operations and short circuit faults cause the generation of the fluctuation on the waveform to be transmitted. Spatial voltage distribution on the single phase transmission line may change owing to the position and duration of the short circuit fault in the system. In this paper, the state space representation of the single phase transmission line for short circuit fault and for various types of terminations is given. Since the transmission line is modeled in time domain using distributed parametric elements, the mathematical representation of the event is given in state space (time domain) differential equation form. It also makes easy to solve the problem because of the time and space dependent characteristics of the voltage variations on the distributed parametrically modeled transmission line. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=energy%20transmission" title="energy transmission">energy transmission</a>, <a href="https://publications.waset.org/abstracts/search?q=transient%20effects" title=" transient effects"> transient effects</a>, <a href="https://publications.waset.org/abstracts/search?q=transmission%20line" title=" transmission line"> transmission line</a>, <a href="https://publications.waset.org/abstracts/search?q=transient%20voltage" title=" transient voltage"> transient voltage</a>, <a href="https://publications.waset.org/abstracts/search?q=RLC%20short%20circuit" title=" RLC short circuit"> RLC short circuit</a>, <a href="https://publications.waset.org/abstracts/search?q=single%20phase" title=" single phase"> single phase</a> </p> <a href="https://publications.waset.org/abstracts/86007/transient-voltage-distribution-on-the-single-phase-transmission-line-under-short-circuit-fault-effect" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86007.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">223</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4644</span> Nucleotide Based Validation of the Endangered Plant Diospyros mespiliformis (Ebenaceae) by Evaluating Short Sequence Region of Plastid rbcL Gene</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20Alaklabi">Abdullah Alaklabi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ibrahim%20A.%20Arif"> Ibrahim A. Arif</a>, <a href="https://publications.waset.org/abstracts/search?q=Sameera%20O.%20Bafeel"> Sameera O. Bafeel</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmad%20H.%20Alfarhan"> Ahmad H. Alfarhan</a>, <a href="https://publications.waset.org/abstracts/search?q=Anis%20Ahamed"> Anis Ahamed</a>, <a href="https://publications.waset.org/abstracts/search?q=Jacob%20Thomas"> Jacob Thomas</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20A.%20Bakir"> Mohammad A. Bakir </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diospyros mespiliformis (Hochst. ex A.DC.; Ebenaceae) is a large deciduous medicinal plant. This plant species is currently listed as endangered in Saudi Arabia. Molecular identification of this plant species based on short sequence regions (571 and 664 bp) of plastid rbcL (ribulose-1, 5-biphosphate carboxylase) gene was investigated in this study. The endangered plant specimens were collected from Al-Baha, Saudi Arabia (GPS coordinate: 19.8543987, 41.3059349). Phylogenetic tree inferred from the rbcL gene sequences showed that this species is very closely related with D. brandisiana. The close relationship was also observed among D. bejaudii, D. Philippinensis and D. releyi (≥99.7% sequence homology). The partial rbcL gene sequence region (571 bp) that was amplified by rbcL primer-pair rbcLaF-rbcLaR failed to discriminate D. mespiliformis from the closely related plant species, D. brandisiana. In contrast, primer-pair rbcL1F-rbcL724R yielded longer amplicon, discriminated the species from D. brandisiana and demonstrated nucleotide variations in 3 different sites (645G>T; 663A>C; 710C>G). Although D. mespiliformis (EU980712) and D. brandisiana (EU980656) are very closely related species (99.4%); however, studied specimen showed 100% sequence homology with D. mespiliformis and 99.6% with D. brandisiana. The present findings showed that rbcL short sequence region (664 bp) of plastid rbcL gene, amplified by primer-pair rbcL1F-rbcL724R, can be used for authenticating samples of D. mespiliforformis and may provide help in authentic identification and management process of this medicinally valuable endangered plant species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Diospyros%20mespiliformis" title="Diospyros mespiliformis">Diospyros mespiliformis</a>, <a href="https://publications.waset.org/abstracts/search?q=endangered%20plant" title=" endangered plant"> endangered plant</a>, <a href="https://publications.waset.org/abstracts/search?q=identification%20partial%20rbcL" title=" identification partial rbcL"> identification partial rbcL</a> </p> <a href="https://publications.waset.org/abstracts/15990/nucleotide-based-validation-of-the-endangered-plant-diospyros-mespiliformis-ebenaceae-by-evaluating-short-sequence-region-of-plastid-rbcl-gene" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15990.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">432</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4643</span> Number of Parametrization of Discrete-Time Systems without Unit-Delay Element: Single-Input Single-Output Case</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kazuyoshi%20Mori">Kazuyoshi Mori</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, we consider the parametrization of the discrete-time systems without the unit-delay element within the framework of the factorization approach. In the parametrization, we investigate the number of required parameters. We consider single-input single-output systems in this paper. By the investigation, we find, on the discrete-time systems without the unit-delay element, three cases that are (1) there exist plants which require only one parameter and (2) two parameters, and (3) the number of parameters is at most three. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=factorization%20approach" title="factorization approach">factorization approach</a>, <a href="https://publications.waset.org/abstracts/search?q=discrete-time%20system" title=" discrete-time system"> discrete-time system</a>, <a href="https://publications.waset.org/abstracts/search?q=parameterization%20of%20stabilizing%20controllers" title=" parameterization of stabilizing controllers"> parameterization of stabilizing controllers</a>, <a href="https://publications.waset.org/abstracts/search?q=system%20without%20unit-delay" title=" system without unit-delay"> system without unit-delay</a> </p> <a href="https://publications.waset.org/abstracts/74319/number-of-parametrization-of-discrete-time-systems-without-unit-delay-element-single-input-single-output-case" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74319.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">240</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4642</span> Vibration Measurements of Single-Lap Cantilevered SPR Beams</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xiaocong%20He">Xiaocong He</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Self-pierce riveting (SPR) is a new high-speed mechanical fastening technique which is suitable for point joining dissimilar sheet materials, as well as coated and pre-painted sheet materials. Mechanical structures assembled by SPR are expected to possess a high damping capacity. In this study, experimental measurement techniques were proposed for the prediction of vibration behavior of single-lap cantilevered SPR beams. The dynamic test software and the data acquisition hardware were used in the experimental measurement of the dynamic response of the single-lap cantilevered SPR beams. Free and forced vibration behavior of the single-lap cantilevered SPR beams was measured using the LMS CADA-X experimental modal analysis software and the LMS-DIFA Scadas II data acquisition hardware. The frequency response functions of the SPR beams of different rivet number were compared. The main goal of the paper is to provide a basic measuring method for further research on vibration based non-destructive damage detection in single-lap cantilevered SPR beams. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=self-piercing%20riveting" title="self-piercing riveting">self-piercing riveting</a>, <a href="https://publications.waset.org/abstracts/search?q=dynamic%20response" title=" dynamic response"> dynamic response</a>, <a href="https://publications.waset.org/abstracts/search?q=experimental%20measurement" title=" experimental measurement"> experimental measurement</a>, <a href="https://publications.waset.org/abstracts/search?q=frequency%20response%20functions" title=" frequency response functions"> frequency response functions</a> </p> <a href="https://publications.waset.org/abstracts/21181/vibration-measurements-of-single-lap-cantilevered-spr-beams" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21181.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">429</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4641</span> The Impact of the European Single Market on the Austrian Economy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reinhard%20Neck">Reinhard Neck</a>, <a href="https://publications.waset.org/abstracts/search?q=Guido%20Sch%C3%A4fer"> Guido Schäfer</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, we explore the macroeconomic effects of the European Single Market on Austria by simulating the McKibbin-Sachs Global Model. Global interdependence and the impact of long-run effects on short-run adjustments are taken into account. We study the sensitivity of the results with respect to different assumptions concerning monetary and fiscal policies for the countries and regions of the world economy. The consequences of different assumptions about budgetary policies in Austria are also investigated. The simulation results are contrasted with ex-post evaluations of the actual impact of Austria’s membership in the Single Market. As a result, it can be concluded that the Austrian participation in the European Single Market entails considerable long-run gains for the Austrian economy with nearly no adverse side-effects on any macroeconomic target variable. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=macroeconomics" title="macroeconomics">macroeconomics</a>, <a href="https://publications.waset.org/abstracts/search?q=European%20Union" title=" European Union"> European Union</a>, <a href="https://publications.waset.org/abstracts/search?q=simulation" title=" simulation"> simulation</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity%20analysis" title=" sensitivity analysis"> sensitivity analysis</a> </p> <a href="https://publications.waset.org/abstracts/29213/the-impact-of-the-european-single-market-on-the-austrian-economy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29213.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">278</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4640</span> LEGO Bricks and Creativity: A Comparison between Classic and Single Sets</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maheen%20Zia">Maheen Zia</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Near the early twenty-first century, LEGO decided to diversify its product range which resulted in more specific and single-outcome sets occupying the store shelves than classic kits having fairly all-purpose bricks. Earlier, LEGOs came with more bricks and lesser instructions. Today, there are more single kits being produced and sold, which come with a strictly defined set of guidelines. If one set is used to make a car, the same bricks cannot be put together to produce any other article. Earlier, multiple bricks gave children a chance to be imaginative, think of new items and construct them (by just putting the same pieces differently). The new products are less open-ended and offer a limited possibility for players in both designing and realizing those designs. The article reviews (in the light of existing research) how classic LEGO sets could help enhance a child’s creativity in comparison with single sets, which allow a player to interact (not experiment) with the bricks. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=constructive%20play" title="constructive play">constructive play</a>, <a href="https://publications.waset.org/abstracts/search?q=creativity" title=" creativity"> creativity</a>, <a href="https://publications.waset.org/abstracts/search?q=LEGO" title=" LEGO"> LEGO</a>, <a href="https://publications.waset.org/abstracts/search?q=play-based%20learning" title=" play-based learning"> play-based learning</a> </p> <a href="https://publications.waset.org/abstracts/130650/lego-bricks-and-creativity-a-comparison-between-classic-and-single-sets" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/130650.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">188</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4639</span> Microstructural and Optical Characterization of Heterostructures of ZnS/CdS and CdS/ZnS Synthesized by Chemical Bath Deposition Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Temesgen%20Geremew">Temesgen Geremew</a> </p> <p class="card-text"><strong>Abstract:</strong></p> ZnS/glass and CdS/glass single layers and ZnS/CdS and CdS/ZnS heterojunction thin films were deposited by the chemical bath deposition method using zinc acetate and cadmium acetate as the metal ion sources and thioacetamide as a nonmetallic ion source in acidic medium. Na2EDTA was used as a complexing agent to control the free cation concentration. +e single layer and heterojunction thin films were characterized with X-ray diffraction (XRD), a scanning electron microscope (SEM), energy dispersive X-ray (EDX), and a UV-VIS spectrometer. +e XRD patterns of the CdS/glass thin film deposited on the soda lime glass substrate crystalized in the cubic structure with a single peak along the (111) plane. +e ZnS/CdS heterojunction and ZnS/glass single layer thin films were crystalized in the hexagonal ZnS structure. +e CdS/ZnS heterojunction thin film is nearly amorphous.The optical analysis results confirmed single band gap values of 2.75 eV and 2.5 eV for ZnS/CdS and CdS/ZnS heterojunction thin films, respectively. +e CdS/glass and CdS/ZnS thin films have more imaginary dielectric components than the real part. The optical conductivity of the single layer and heterojunction films is in the order of 1015 1/s. +e optical study also confirmed refractive index values between 2 and 2.7 for ZnS/glass, ZnS/CdS, and CdS/ZnS thin films for incident photon energies between 1.2 eV and 3.8 eV. +e surface morphology studies revealed compacted spherical grains covering the substrate surfaces with few cracks on ZnS/glass, ZnS/CdS, and CdS/glass and voids on CdS/ZnS thin films. +e EDX result confirmed nearly 1 :1 metallic to nonmetallic ion ratio in the single-layered thin films and the dominance of Zn ion over Cd ion in both ZnS/CdS and CdS/ZnS heterojunction thin films. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=SERS" title="SERS">SERS</a>, <a href="https://publications.waset.org/abstracts/search?q=sensor" title=" sensor"> sensor</a>, <a href="https://publications.waset.org/abstracts/search?q=Hg2%2B" title=" Hg2+"> Hg2+</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20detection" title=" water detection"> water detection</a>, <a href="https://publications.waset.org/abstracts/search?q=polythiophene" title=" polythiophene"> polythiophene</a> </p> <a href="https://publications.waset.org/abstracts/178851/microstructural-and-optical-characterization-of-heterostructures-of-znscds-and-cdszns-synthesized-by-chemical-bath-deposition-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/178851.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">65</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4638</span> Maximum-likelihood Inference of Multi-Finger Movements Using Neural Activities</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kyung-Jin%20You">Kyung-Jin You</a>, <a href="https://publications.waset.org/abstracts/search?q=Kiwon%20Rhee"> Kiwon Rhee</a>, <a href="https://publications.waset.org/abstracts/search?q=Marc%20H.%20Schieber"> Marc H. Schieber</a>, <a href="https://publications.waset.org/abstracts/search?q=Nitish%20V.%20Thakor"> Nitish V. Thakor</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyun-Chool%20Shin">Hyun-Chool Shin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> It remains unknown whether M1 neurons encode multi-finger movements independently or as a certain neural network of single finger movements although multi-finger movements are physically a combination of single finger movements. We present an evidence of correlation between single and multi-finger movements and also attempt a challenging task of semi-blind decoding of neural data with minimum training of the neural decoder. Data were collected from 115 task-related neurons in M1 of a trained rhesus monkey performing flexion and extension of each finger and the wrist (12 single and 6 two-finger-movements). By exploiting correlation of temporal firing pattern between movements, we found that correlation coefficient for physically related movements pairs is greater than others; neurons tuned to single finger movements increased their firing rate when multi-finger commands were instructed. According to this knowledge, neural semi-blind decoding is done by choosing the greatest and the second greatest likelihood for canonical candidates. We achieved a decoding accuracy about 60% for multiple finger movement without corresponding training data set. this results suggest that only with the neural activities on single finger movements can be exploited to control dexterous multi-fingered neuroprosthetics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=finger%20movement" title="finger movement">finger movement</a>, <a href="https://publications.waset.org/abstracts/search?q=neural%20activity" title=" neural activity"> neural activity</a>, <a href="https://publications.waset.org/abstracts/search?q=blind%20decoding" title=" blind decoding"> blind decoding</a>, <a href="https://publications.waset.org/abstracts/search?q=M1" title=" M1"> M1</a> </p> <a href="https://publications.waset.org/abstracts/1874/maximum-likelihood-inference-of-multi-finger-movements-using-neural-activities" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/1874.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">320</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4637</span> Studies on Phylogeny of Helicoverpa armigera Populations from North Western Himalaya Region with Help of Cytochromeoxidase I Sequence</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=R.%20M.%20Srivastava">R. M. Srivastava</a>, <a href="https://publications.waset.org/abstracts/search?q=Subbanna%20A.R.N.S"> Subbanna A.R.N.S</a>, <a href="https://publications.waset.org/abstracts/search?q=Md%20Abbas%20Ahmad"> Md Abbas Ahmad</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20P.More"> S. P.More</a>, <a href="https://publications.waset.org/abstracts/search?q=Shivashankar"> Shivashankar</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Kalyanbabu">B. Kalyanbabu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The similar morphology associated with high genetic variability poses problems in phylogenetic studies of Helicoverpa armigera (Hubner). To identify genetic variation of North Western Himalayan population’s, partial (Mid to terminal region) cytochrome c oxidase subunit I (COX-1) gene was amplified and sequenced for three populations collected from Pantnagar, Almora, and Chinyalisaur. The alignment of sequences with other two populations, Nagpur representing central India population and Anhui, China representing complete COX-1 sequence revealed unanimity in middle region with eleven single nucleotide polymorphisms (SNPs) in Nagpur populations. However, the consensus is missing when approaching towards terminal region, which is associated with 15 each SNPs and pair base substitutions in Chinyalisaur populations. In minimum evolution tree, all the five populations were majorly separated into two clades, one comprising of only Nagpur population and the other with rest. Amongst, North Western populations, Chinyalisaur one is promising by farming a separate clade. The pairwise genetic distance ranges from 0.025 to 0.192 with the maximum between H. armigera populations of Nagpur and Chinyalisaur. This genetic isolation of populations can be attributed to a key role of topological barriers of weather and mountain ranges and temporal barriers due to cropping patterns. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytochrome%20c%20oxidase%20subunit%20I" title="cytochrome c oxidase subunit I">cytochrome c oxidase subunit I</a>, <a href="https://publications.waset.org/abstracts/search?q=northwestern%20Himalayan%20population" title=" northwestern Himalayan population"> northwestern Himalayan population</a>, <a href="https://publications.waset.org/abstracts/search?q=Helicoverpa%20armigera%20%28Noctuidae%3A%20Lepidoptera%29" title=" Helicoverpa armigera (Noctuidae: Lepidoptera)"> Helicoverpa armigera (Noctuidae: Lepidoptera)</a>, <a href="https://publications.waset.org/abstracts/search?q=phylogenetic%20relationship" title=" phylogenetic relationship"> phylogenetic relationship</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20variation" title=" genetic variation"> genetic variation</a> </p> <a href="https://publications.waset.org/abstracts/71872/studies-on-phylogeny-of-helicoverpa-armigera-populations-from-north-western-himalaya-region-with-help-of-cytochromeoxidase-i-sequence" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/71872.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">309</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4636</span> Use of RAPD and ISSR Markers in Detection of Genetic Variation among Colletotrichum falcatum Went Isolates from South Gujarat India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Prittesh%20Patel">Prittesh Patel</a>, <a href="https://publications.waset.org/abstracts/search?q=Rushabh%20Shah"> Rushabh Shah</a>, <a href="https://publications.waset.org/abstracts/search?q=Krishnamurthy%20Ramar"> Krishnamurthy Ramar</a>, <a href="https://publications.waset.org/abstracts/search?q=Vakulbhushan%20Bhaskar"> Vakulbhushan Bhaskar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present research work aims at finding genetic differences in the genomes of sugarcane red rot isolates Colletotrichum falcatum Went using Random Amplified Polymorphic DNA (RAPD) and interspersed simple sequence repeat (ISSR) molecular markers. Ten isolates of C. falcatum isolated from different red rot infected sugarcane cultivars stalk were used in present study. The amplified bands were scored across the lanes obtained in 15 RAPD primes and 21 ISSR primes successfully. The data were analysed using NTSYSpc 2.2 software. The results showed 80.6% and 68.07% polymorphism in RPAD and ISSR analysis respectively. Based on the RAPD analysis, ten genotypes were grouped into two major clusters at a cut-off value of 0.75. Geographically distant C. falcatum isolate cfGAN from south Gujarat had a level of similarity with Coimbatore isolate cf8436 presented on separate clade of bootstrapped dendrograms. First and second cluster consisted of five and three isolates respectively, indicating the close relation among them. The 21 ISSR primers produced 119 distinct and scorable loci in that 38 were monomorphic. The number of scorable loci for each primer varied from 2 (ISSR822) to 8 (ISSR807, ISSR823 and ISSR15) with an average of 5.66 loci per primer. Primer ISSR835 amplified the highest number of bands (57), while only 16 bands were obtained by primers ISSR822. Four primers namely ISSR830, ISSR845, ISSR4 and ISSR15 showed the highest value of percentage of polymorphism (100%). The results indicated that both of the marker systems RAPD and ISSR, individually can be effectively used in determination of genetic relationship among C falcatum accessions collected from different parts of south Gujarat. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Colletotrichum%20falcatum" title="Colletotrichum falcatum">Colletotrichum falcatum</a>, <a href="https://publications.waset.org/abstracts/search?q=ISSR" title=" ISSR"> ISSR</a>, <a href="https://publications.waset.org/abstracts/search?q=RAPD" title=" RAPD"> RAPD</a>, <a href="https://publications.waset.org/abstracts/search?q=Red%20Rot" title=" Red Rot"> Red Rot</a> </p> <a href="https://publications.waset.org/abstracts/66245/use-of-rapd-and-issr-markers-in-detection-of-genetic-variation-among-colletotrichum-falcatum-went-isolates-from-south-gujarat-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66245.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">361</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4635</span> Torque Magnetometry of Low Anisotropic CaCo2As2 Single Crystals</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kashif%20Nadeem">Kashif Nadeem</a>, <a href="https://publications.waset.org/abstracts/search?q=W.%20Zhang"> W. Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=X.%20G.%20Qiu"> X. G. Qiu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Role of Co spins in CaCo2As2 single crystal is systematically studied by using dc magnetization and magnetic torque measurements. A spin-flop transition in the antiferromagnetism (AFM) CaCo2As2 single crystal is studied by using dc magnetization and magnetic torque. Field dependent and angle dependent torque magnetometry confirmed the existence of spin-flop transition in this compound which is in agreement with the dc magnetization studies. A comparison of dc magnetization and torque magnetometry measurements for CaCo2As2 single crystal is done in detail. In conclusion, torque magnetometry can be a useful tool to study the spin flop transition in low anisotropic compounds analogous to dc magnetization studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=spin%20flop%20transition" title="spin flop transition">spin flop transition</a>, <a href="https://publications.waset.org/abstracts/search?q=torque%20magnetometry" title=" torque magnetometry"> torque magnetometry</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetization" title=" magnetization"> magnetization</a>, <a href="https://publications.waset.org/abstracts/search?q=anisotropic" title=" anisotropic"> anisotropic</a> </p> <a href="https://publications.waset.org/abstracts/24659/torque-magnetometry-of-low-anisotropic-caco2as2-single-crystals" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24659.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">548</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4634</span> In vivo Estimation of Mutation Rate of the Aleutian Mink Disease Virus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.P.%20Rupasinghe">P.P. Rupasinghe</a>, <a href="https://publications.waset.org/abstracts/search?q=A.H.%20Farid"> A.H. Farid</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Aleutian mink disease virus (AMDV, Carnivore amdoparvovirus 1) causes persistent infection, plasmacytosis, and formation and deposition of immune complexes in various organs in adult mink, leading to glomerulonephritis, arteritis and sometimes death. The disease has no cure nor an effective vaccine, and identification and culling of mink positive for anti-AMDV antibodies have not been successful in controlling the infection in many countries. The failure to eradicate the virus from infected farms may be caused by keeping false-negative individuals on the farm, virus transmission from wild animals, or neighboring farms. The identification of sources of infection, which can be performed by comparing viral sequences, is important in the success of viral eradication programs. High mutation rates could cause inaccuracies when viral sequences are used to trace back an infection to its origin. There is no published information on the mutation rate of AMDV either in vivo or in vitro. The in vivo estimation is the most accurate method, but it is difficult to perform because of the inherent technical complexities, namely infecting live animals, the unknown numbers of viral generations (i.e., infection cycles), the removal of deleterious mutations over time and genetic drift. The objective of this study was to determine the mutation rate of AMDV on which no information was available. A homogenate was prepared from the spleen of one naturally infected American mink (Neovison vison) from Nova Scotia, Canada (parental template). The near full-length genome of this isolate (91.6%, 4,143 bp) was bidirectionally sequenced. A group of black mink was inoculated with this homogenate (descendant mink). Spleen sampled were collected from 10 descendant mink after 16 weeks post-inoculation (wpi) and from anther 10 mink after 176 wpi, and their near-full length genomes were bi-directionally sequenced. Sequences of these mink were compared with each other and with the sequence of the parental template. The number of nucleotide substitutions at 176 wpi was 3.1 times greater than that at 16 wpi (113 vs 36) whereas the estimates of mutation rate at 176 wpi was 3.1 times lower than that at 176 wpi (2.85×10-3 vs 9.13×10-4 substitutions/ site/ year), showing a decreasing trend in the mutation rate per unit of time. Although there is no report on in vivo estimate of the mutation rate of DNA viruses in animals using the same method which was used in the current study, these estimates are at the higher range of reported values for DNA viruses determined by various techniques. These high estimates are logical based on the wide range of diversity and pathogenicity of AMDV isolates. The results suggest that increases in the number of nucleotide substitutions over time and subsequent divergence make it difficult to accurately trace back AMDV isolates to their origin when several years elapsed between the two samplings. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aleutian%20mink%20disease%20virus" title="Aleutian mink disease virus">Aleutian mink disease virus</a>, <a href="https://publications.waset.org/abstracts/search?q=American%20mink" title=" American mink"> American mink</a>, <a href="https://publications.waset.org/abstracts/search?q=mutation%20rate" title=" mutation rate"> mutation rate</a>, <a href="https://publications.waset.org/abstracts/search?q=nucleotide%20substitution" title=" nucleotide substitution"> nucleotide substitution</a> </p> <a href="https://publications.waset.org/abstracts/124545/in-vivo-estimation-of-mutation-rate-of-the-aleutian-mink-disease-virus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/124545.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">125</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4633</span> Molecular Insights into the Genetic Integrity of Long-Term Micropropagated Clones Using Start Codon Targeted (SCoT) Markers: A Case Study with Ansellia africana, an Endangered, Medicinal Orchid</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Paromik%20Bhattacharyya">Paromik Bhattacharyya</a>, <a href="https://publications.waset.org/abstracts/search?q=Vijay%20Kumar"> Vijay Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Johannes%20Van%20Staden"> Johannes Van Staden</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Micropropagation is an important tool for the conservation of threatened and commercially important plant species of which orchids deserve special attention. Ansellia africana is one such medicinally important orchid species having much commercial significance. Thus, development of regeneration protocols for producing clonally stable regenerates using axillary buds is of much importance. However, for large-scale micropropagation to become not only successful but also acceptable by end-users, somaclonal variations occurring in the plantlets need to be eliminated. In the light of the various factors (genotype, ploidy level, in vitro culture age, explant and culture type, etc.) that may account for the somaclonal variations of divergent genetic changes at the cellular and molecular levels, genetic analysis of micropropagated plants using a multidisciplinary approach is of utmost importance. In the present study, the clonal integrity of the long term micropropagated A. africana plants were assessed using advanced molecular marker system i.e. Start Codon Targeted Polymorphism (SCoT). Our studies recorded a clonally stable regeneration protocol for A. africana with a very high degree of clonal fidelity amongst the regenerates. The results obtained from these molecular analyses could help in modifying the regeneration protocols for obtaining clonally stable true to type plantlets for sustainable commercial use. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=medicinal%20orchid%20micropropagation" title="medicinal orchid micropropagation">medicinal orchid micropropagation</a>, <a href="https://publications.waset.org/abstracts/search?q=start%20codon%20targeted%20polymorphism%20%28SCoT%29" title=" start codon targeted polymorphism (SCoT)"> start codon targeted polymorphism (SCoT)</a>, <a href="https://publications.waset.org/abstracts/search?q=RAP%29" title="RAP)">RAP)</a>, <a href="https://publications.waset.org/abstracts/search?q=traditional%20African%20pharmacopoeia" title=" traditional African pharmacopoeia"> traditional African pharmacopoeia</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20%20fidelity" title=" genetic fidelity"> genetic fidelity</a> </p> <a href="https://publications.waset.org/abstracts/53547/molecular-insights-into-the-genetic-integrity-of-long-term-micropropagated-clones-using-start-codon-targeted-scot-markers-a-case-study-with-ansellia-africana-an-endangered-medicinal-orchid" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53547.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">426</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4632</span> Comparison between LQR and ANN Active Anti-Roll Control of a Single Unit Heavy Vehicle </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Babesse%20Saad">Babesse Saad</a>, <a href="https://publications.waset.org/abstracts/search?q=Ameddah%20Djemeleddine"> Ameddah Djemeleddine</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, a learning algorithm using neuronal networks to improve the roll stability and prevent the rollover in a single unit heavy vehicle is proposed. First, LQR control to keep balanced normalized rollovers, between front and rear axles, below the unity, then a data collected from this controller is used as a training basis of a neuronal regulator. The ANN controller is thereafter applied for the nonlinear side force model, and gives satisfactory results than the LQR one. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rollover" title="rollover">rollover</a>, <a href="https://publications.waset.org/abstracts/search?q=single%20unit%20heavy%20vehicle" title=" single unit heavy vehicle"> single unit heavy vehicle</a>, <a href="https://publications.waset.org/abstracts/search?q=neural%20networks" title=" neural networks"> neural networks</a>, <a href="https://publications.waset.org/abstracts/search?q=nonlinear%20side%20force" title=" nonlinear side force "> nonlinear side force </a> </p> <a href="https://publications.waset.org/abstracts/13730/comparison-between-lqr-and-ann-active-anti-roll-control-of-a-single-unit-heavy-vehicle" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13730.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">474</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4631</span> MPC of Single Phase Inverter for PV System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Irtaza%20M.%20Syed">Irtaza M. Syed</a>, <a href="https://publications.waset.org/abstracts/search?q=Kaamran%20Raahemifar"> Kaamran Raahemifar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper presents a model predictive control (MPC) of a utility interactive (UI) single phase inverter (SPI) for a photovoltaic (PV) system at residential/distribution level. The proposed model uses single-phase phase locked loop (PLL) to synchronize SPI with the grid and performs MPC control in a dq reference frame. SPI model consists of boost converter (BC), maximum power point tracking (MPPT) control, and a full bridge (FB) voltage source inverter (VSI). No PI regulators to tune and carrier and modulating waves are required to produce switching sequence. Instead, the operational model of VSI is used to synthesize sinusoidal current and track the reference. Model is validated using a three kW PV system at the input of UI-SPI in Matlab/Simulink. Implementation and results demonstrate simplicity and accuracy, as well as reliability of the model. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phase%20locked%20loop" title="phase locked loop">phase locked loop</a>, <a href="https://publications.waset.org/abstracts/search?q=voltage%20source%20inverter" title=" voltage source inverter"> voltage source inverter</a>, <a href="https://publications.waset.org/abstracts/search?q=single%20phase%20inverter" title=" single phase inverter"> single phase inverter</a>, <a href="https://publications.waset.org/abstracts/search?q=model%20predictive%20control" title=" model predictive control"> model predictive control</a>, <a href="https://publications.waset.org/abstracts/search?q=Matlab%2FSimulink" title=" Matlab/Simulink"> Matlab/Simulink</a> </p> <a href="https://publications.waset.org/abstracts/16006/mpc-of-single-phase-inverter-for-pv-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16006.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">532</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4630</span> Impact of Vehicle Travel Characteristics on Level of Service: A Comparative Analysis of Rural and Urban Freeways</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anwaar%20Ahmed">Anwaar Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Bilal%20Khurshid"> Muhammad Bilal Khurshid</a>, <a href="https://publications.waset.org/abstracts/search?q=Samuel%20Labi"> Samuel Labi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The effect of trucks on the level of service is determined by considering passenger car equivalents (PCE) of trucks. The current version of Highway Capacity Manual (HCM) uses a single PCE value for all tucks combined. However, the composition of truck traffic varies from location to location; therefore a single PCE-value for all trucks may not correctly represent the impact of truck traffic at specific locations. Consequently, present study developed separate PCE values for single-unit and combination trucks to replace the single value provided in the HCM on different freeways. Site specific PCE values, were developed using concept of spatial lagging headways (the distance from the rear bumper of a leading vehicle to the rear bumper of the following vehicle) measured from field traffic data. The study used data from four locations on a single urban freeway and three different rural freeways in Indiana. Three-stage-least-squares (3SLS) regression techniques were used to generate models that predicted lagging headways for passenger cars, single unit trucks (SUT), and combination trucks (CT). The estimated PCE values for single-unit and combination truck for basic urban freeways (level terrain) were: 1.35 and 1.60, respectively. For rural freeways the estimated PCE values for single-unit and combination truck were: 1.30 and 1.45, respectively. As expected, traffic variables such as vehicle flow rates and speed have significant impacts on vehicle headways. Study results revealed that the use of separate PCE values for different truck classes can have significant influence on the LOS estimation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=level%20of%20service" title="level of service">level of service</a>, <a href="https://publications.waset.org/abstracts/search?q=capacity%20analysis" title=" capacity analysis"> capacity analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=lagging%20headway" title=" lagging headway"> lagging headway</a>, <a href="https://publications.waset.org/abstracts/search?q=trucks" title=" trucks"> trucks</a> </p> <a href="https://publications.waset.org/abstracts/10791/impact-of-vehicle-travel-characteristics-on-level-of-service-a-comparative-analysis-of-rural-and-urban-freeways" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10791.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">355</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4629</span> In-situ Observations Using SEM-EBSD for Bending Deformation in Single-Crystal Materials</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yuko%20Matayoshi">Yuko Matayoshi</a>, <a href="https://publications.waset.org/abstracts/search?q=Takashi%20Sakai"> Takashi Sakai</a>, <a href="https://publications.waset.org/abstracts/search?q=Yin-Gjum%20Jin"> Yin-Gjum Jin</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun-ichi%20Koyama"> Jun-ichi Koyama</a> </p> <p class="card-text"><strong>Abstract:</strong></p> To elucidate the material characteristics of single crystals of pure aluminum and copper, the respective relations between crystallographic orientations and micro structures were examined, along with bending and mechanical properties. The texture distribution was also analysed. Bending tests were performed in a SEM apparatus while its behaviors were observed. Some analytical results related to crystal direction maps, inverse pole figures, and textures were obtained from electron back scatter diffraction (EBSD) analyses. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pure%20aluminum" title="pure aluminum">pure aluminum</a>, <a href="https://publications.waset.org/abstracts/search?q=pure%20copper" title=" pure copper"> pure copper</a>, <a href="https://publications.waset.org/abstracts/search?q=single%20crystal" title=" single crystal"> single crystal</a>, <a href="https://publications.waset.org/abstracts/search?q=bending" title=" bending"> bending</a>, <a href="https://publications.waset.org/abstracts/search?q=SEM-EBSD%20analysis" title=" SEM-EBSD analysis"> SEM-EBSD analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=texture" title=" texture"> texture</a>, <a href="https://publications.waset.org/abstracts/search?q=microstructure" title=" microstructure"> microstructure</a> </p> <a href="https://publications.waset.org/abstracts/16499/in-situ-observations-using-sem-ebsd-for-bending-deformation-in-single-crystal-materials" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16499.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">368</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=single%20nucleotide%20polymorphism&page=7" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=single%20nucleotide%20polymorphism&page=1">1</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=single%20nucleotide%20polymorphism&page=2">2</a></li> <li class="page-item 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