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style="background-image:url(https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2022/06/PG2631-PJT9046-COL020411-PCA-Immunology-campaign_Protocol-handbook-blog-cover-image-Global-1600x350-F.jpg)"> </section> <div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-16780 post type-post status-publish format-standard has-post-thumbnail category-immunology tag-flow-cytometry tag-immunology entry" aria-label="Recipe for Success"><header class="entry-header"><h1 class="entry-title">Recipe for Success</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/behind-the-bench-staff/" title="Posts by Behind The Bench Staff" rel="author">Behind The Bench Staff</a> </span> <span class="post-info-date"> 06.27.2022 </span> </header><div class="entry-content"><h1><strong>Why are Protocols Hard to Find?</strong></h1> <p>Immunology research is advancing by leaps and bounds. Hence, the need to plan experiments better. Flow cytometry is an extremely useful, yet complex cell analysis technique. It has many different components to planning flow cytometry experiments – antibodies and fluorophores, instrument optical configurations, sample preparation, just to name a few. However, one other component which often needs a lot of attention is protocols. Scientists spend a lot of their valuable time sorting through heaps of research papers and other literature looking for protocols.</p> <p>There are so many sources, none of them are complete. It’s hard to sort through them all to determine which sources have reliable and relevant info to the experiment at hand. Moreover, if a given flow cytometry protocol does not work, scientists must repeat the process multiple times to narrow down what works best. Much like cooking, you have all required ingredients and know your goals but choosing the right process is a key for success. Once you perfect the recipe you want reproducibility and consistency, right?</p> <p>This was just about finding one protocol. But we know that in the real world, scientists are running multiple assays simultaneously, which multiplies their challenges. Also, let us not forget that immunology is very dynamic where new data is constantly pouring in, and scientists need to stay abreast of the developments in their fields.</p> <h2><strong>Finding the Right Solution</strong></h2> <p>What if there was a financial advisor who does the research for you and lays out all investment options on the table to choose from? Sounds convenient, right? Similarly, <a href="https://www.thermofisher.com/us/en/home/global/forms/flow-cytometry-protocols-handbook.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020411_0s0_blg_op_awa_kt_s00_assay1">Flow Cytometry Protocols Handbook</a> is that advisor for immunology scientists. It is an all-in-one handbook that could accelerate your research by helping you plan and set-up experiments. The handbook covers main steps in flow cytometry protocols such as sample preparation, immune cell stimulation, phenotypic analysis, cell viability and apoptosis, cell cycle, and cell proliferation.</p> <p>Moreover, there is a whole library of resources available to scientists called <a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/immunology-at-work.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020411_0s0_blg_op_awa_kt_s00_assay2">Immunology at Work Resource Center</a>. It provides practical guidance including basics review, acquire in-depth knowledge, or discovering latest research tools. The content on this hub is created by expert scientists to help new and experienced scientists in immunology.</p> <h2><strong>Looking In the Right Place</strong></h2> <p>Protocol is like a recipe for running a quality experiment. It needs to be trustworthy and thorough to define all important steps appropriately. Finding such quality protocols can be challenging. Scientists can alleviate some of their concerns about finding protocols and technical resources if they know where to look. Time and effort saved due to these resources can be spent on core research leading to discovery. Especially, when a team of trusted experts have compiled wide range of flow cytometry protocols. Having an easy access to such a useful resource can be truly convenient and leave you with one less factor to worry about!</p> <p><img decoding="async" class="alignnone wp-image-16781 size-large" src="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2022/06/PG2631-PJT9046-COL020411-PCA-Immunology-campaign_Protocol-handbook-blog-cover-image-Global-1600x350-F-1024x224.jpg" alt="Flow cytometry protocols handbook" width="760" height="166" srcset="https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2022/06/PG2631-PJT9046-COL020411-PCA-Immunology-campaign_Protocol-handbook-blog-cover-image-Global-1600x350-F-1024x224.jpg 1024w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2022/06/PG2631-PJT9046-COL020411-PCA-Immunology-campaign_Protocol-handbook-blog-cover-image-Global-1600x350-F-300x66.jpg 300w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2022/06/PG2631-PJT9046-COL020411-PCA-Immunology-campaign_Protocol-handbook-blog-cover-image-Global-1600x350-F-768x168.jpg 768w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2022/06/PG2631-PJT9046-COL020411-PCA-Immunology-campaign_Protocol-handbook-blog-cover-image-Global-1600x350-F-1536x336.jpg 1536w, https://www.thermofisher.com/blog/behindthebench/wp-content/uploads/sites/9/2022/06/PG2631-PJT9046-COL020411-PCA-Immunology-campaign_Protocol-handbook-blog-cover-image-Global-1600x350-F.jpg 1600w" sizes="(max-width: 760px) 100vw, 760px" /></p> <h3><strong>Download the</strong> <a href="https://www.thermofisher.com/us/en/home/global/forms/flow-cytometry-protocols-handbook.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020411_0s0_blg_op_awa_kt_s00_assay3">Flow Cytometry Protocols Handbook</a></h3> <h2><strong>Flow cytometry protocol resources and solutions</strong></h2> <ul> <li><a href="https://www.thermofisher.com/us/en/home/global/forms/flow-cytometry-protocols-handbook.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020411_0s0_blg_op_awa_kt_s00_assay4">Flow Cytometry Protocols Handbook</a> – protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping and data analysis strategies</li> <li><a href="https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/immunology-at-work.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020411_0s0_blg_op_awa_kt_s00_assay5">Immunology at Work Resource Center</a> – practical guidance and protocols to help you get started quickly and confidently</li> </ul> <h2><strong>References</strong></h2> <ul> <li><a href="https://ccmorey.github.io/labHandbook/experimental-protocols.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020411_0s0_blg_op_awa_kt_s00_assay6">https://ccmorey.github.io/labHandbook/experimental-protocols.html</a></li> </ul> <h2><strong>Additional blogs:</strong></h2> <ul> <li><a href="https://www.thermofisher.com/blog/behindthebench/how-cell-images-enable-more-accuracy-in-flow-cytometry-data/">How Cell Images Enable More Accuracy in Flow Cytometry Data?</a></li> <li><a href="https://www.thermofisher.com/blog/behindthebench/standardization-challenges-how-to-compare-flow-cytometry-data-across-experiments/">Standardization Challenges: How to Compare Your Flow Cytometry Data Across Experiments?</a></li> <li><a href="https://www.thermofisher.com/blog/behindthebench/fast-flow-cytometry-assisted-antimicrobial-susceptibility-testing/">FAST: Flow Cytometry-Assisted Antimicrobial Susceptibility Testing</a></li> <li><a href="https://www.thermofisher.com/blog/behindthebench/tnkupj-novel-anti-mouse-cd163-monoclonal-antibody-macrophages-flow-cytometry/">Anti-mouse CD163: A macrophage marker for flow cytometry</a></li> </ul> <aside class="mashsb-container mashsb-main "><div class="mashsb_above_buttons">Share this article </div><div class="mashsb-box"><div class="mashsb-buttons"><a class="mashicon-facebook mash-large mashsb-noshadow" href="https://www.facebook.com/sharer.php?u=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fflow-cytometry-protocol-design%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Facebook</span></a><a class="mashicon-linkedin mash-large mashsb-noshadow" href="https://www.linkedin.com/shareArticle?trk=Recipe+for+Success&url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fbehindthebench%2Fflow-cytometry-protocol-design%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Linkedin</span></a><a class="mashicon-twitter 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