CINXE.COM

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Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> <img src="//images.novusbio.com/images/antibody/mab3075_human-sox9-mab-clone-326820-immunocytochemistry-216202394839..jpg" title="SOX9 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HEL 92.1.7 human erythroleukemic cells (negative) using Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="SOX9 was detected in immersion fixed U‑251 MG human glioblastoma cells (positive) and absent in HEL 92.1.7 human erythroleukemic cells (negative) using Mouse Anti-Human SOX9 Monoclonal Antibody (Catalog # MAB3075) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" /> </a> </div></div><div class="pager_fill"></div><div id="image-caption-MAB3075"></div> </div><div class="review_publication_wrapper forcol3"></div> <div class="product-attributes"><div class="field not3column"><label>Reactivity:</label><div class="value"><span title="Human">Hu</span><div class="myModal">Species Glossary</div></div></div><div class="forcol3 bottom_margin"><strong>Reactivity:</strong> <span title="Human">Hu</span></div><div class="field not3column"><label>Applications:</label><div class="value"><span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span></div></div><div class="forcol3 bottom_margin"><strong>Applications:</strong> <span class="application-tooltip-text" title="Immunocytochemistry/ Immunofluorescence">ICC/IF</span></div><div class="field not3column"><label>Host:</label><div class="value">Mouse Monoclonal</div></div><div class="forcol3 bottom_margin"><strong>Host:</strong> Mouse Monoclonal</div><div class="field not3column"><label>Sizes:</label><div class="value">Sample Size Available</div></div><div class="forcol3 bottom_margin"><strong>Sizes:</strong> Sample Size Available</div><div class="field not3column"><label>Conjugate:</label><div class="value">Unconjugated  </div></div> </div><div><div class="field not3column"><label>&nbsp</label><div class="value"><div class="list_expand not3column options_closed" data-name="Formulations" data-wrapper-type=".formulations_wrapper" style="margin-left:0px;">Available Formulations <div class="expander option_closed"></div></div></div></div></div><div class="forcol3 option_buttons_block"><button class="options_button" data-type="formulations">Formulations</button></div><div class="formulations_wrapper" data-title="SOX9 Antibody (326820) [Unconjugated]" data-catnum="MAB3075"><table class="scf_table"> <thead> <tr> <th>Formulation</th> <th>Catalog #</th> 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P48436","Citations":"-","Purity":"","Activity":"","Accession #":"","N-terminal Sequence Analysis":"","Predicated Molecular Mass":"","SDS Page":"","Assay Type":"","Format":"","Assay Length":"","Sample Type and Volume Required":"","Sensitivity":"","Interference":"","Datasheet":"<a href=\"\/products\/sox9-antibody-326820_mab3075\">view datasheet<\/a>","Price":"Price Not Shown"}'><div class="search_compare">compare</div> <div class="search_compare_text">(select up to 3 products from this page to compare)</div> </div> <div class="clear"></div> </div></div><div class="lower_framework"> <div class="search_pager"><div class="searchImage_backGrey" style="display:inline-block;zoom:1;width:6px;height:11px;background:url(//resources.rndsystems.com/images/search/searchSprite03-31-16.png) -18px -169px"></div> page <input type="text" value="1" class="search_pager_text" data-url="/search?keywords=SOX1&showGrid=true&modification=No%20Modification&clone=326820&has_sample_size=true&page="> of <span 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