CINXE.COM

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class="ds_title_table"> <h1 class="ds_title">Human BMI-1 Alexa Fluor® 750-conjugated Antibody </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: IC33341S</span> <a href="/reviews/IC33341S" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/human-bmi-1-alexa-fluor-750-conjugated-antibody-384515_ic33341s" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog #</th> <th class="ds_availability_column mobile-hide">Availability</th> <th 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onClick="event.preventDefault();" data-title="R&D Systems Antibodies" data-footer="R&D Systems Antibodies" title="Cat. No. IC33341S - Human BMI-1 Alexa Fluor® 750-conjugated Antibody" href="https://resources.rndsystems.com/categoryimages/rndsystems_antibodies.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/categoryimages/rndsystems_antibodies.jpg" class="img-fluid" alt="R&D Systems Antibodies" title="R&D Systems Antibodies"> </a> <div class="search_image_other_count"> <span class="search_more_images">1 Image</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/bmi-1?category=Primary Antibodies" class="btn btn-secondary">All BMI-1 Antibodies</a> </div> </div> <div class="tabbed_content" id="rnd_antibody"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_calculator" id="product-anchor-ds_calculator">Reconstitution Calculator</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <h2 id="ds_summary">Human BMI-1 Alexa Fluor® 750-conjugated Antibody Summary</h2> <div class="datasheet_section"> <div class="ds_table summary_table"> <div class="ds_table_body">  <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Species Reactivity</div> <div class="ds_table_cell summary_table_data"> Human </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data"> Detects human BMI-1 in direct ELISAs and Western blots. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Source</div> <div class="ds_table_cell summary_table_data"> Monoclonal Mouse IgG2a Clone # 384515 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Immunogen</div> <div class="ds_table_cell summary_table_data"> <EM>E. coli</EM>-derived recombinant human BMI-1<br />Asp96-Gly326<br />Accession # P35226 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Formulation</div> <div class="ds_table_cell summary_table_data"> Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Label</div> <div class="ds_table_cell summary_table_data"> Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm) </div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="IC33341S"></div> <div id="ds_applications" class="datasheet_section"> <h2>Applications</h2> <div class="ds_table app_table"> <div class="ds_table_head"> <div class="ds_table_head_row"> <div class="ds_table_head_cell app_table_blank"></div> <div class="ds_table_head_cell app_table_rec_conc">Recommended Concentration</div> <div class="ds_table_head_cell app_table_sample">Sample</div> </div> </div> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell app_table_name">Intracellular Staining by Flow Cytometry</div> <div class="ds_table_cell app_table_dilution">0.25-1 µg/10<sup>6</sup> cells</div> <div class="ds_table_cell app_table_sample">HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin</div> </div> </div> </div> <p>Please Note: Optimal dilutions should be determined by each laboratory for each application. <a href="/resources/protocols-troubleshooting-guides">General Protocols</a> are available in the Technical Information section on our website. </p> </div> <div id="ds_calculator" class="datasheet_section"> <div id="btn_calculator_wrapper"><div class="datasheet_calculator_toggle btn btn-secondary" id="btn_reconstitution">Reconstitution Calculator</div></div><div id="datasheet_calculators"><div id="btn_reconstitution_calc" class="datasheet_calculator"><h2>Reconstitution Calculator</h2><div id="reconstitution_calculator" class="calculator"> <form id="recCalc"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume (to add to vial)</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </span> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass (in vial)</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </span> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Desired Reconstitution Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="conc." class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </span> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button btn btn-secondary"/> </div> </form> </div> </div></div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Stability & Storage</div> <div class="ds_table_cell prep_table_data">Store the unopened product at 2 - 8 °C. Do not use past expiration date. </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: BMI-1</h2> <p><p>BMI-1 (<u>B</u> cell-specific <u>M</u>oloney-MLV <u>i</u>ntegration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 shares 99%, 97%, 99% and 99% aa sequence identity with bovine, mouse, feline and canine BMI-1, respectively.</p></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name</div> <div class="ds_table_cell background_table_data">B Lymphoma Mo-MLV Insertion Region 1</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs</div> <div class="ds_table_cell background_table_data">648 (Human); 12151 (Mouse)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names</div> <div class="ds_table_cell background_table_data">B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4</div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/ic33341s.pdf?v=20241105" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-bmi-1-alexa-fluor-750-conjugated-antibody-384515_ic33341s">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/ic33341s?v=20241105">SDS</a> </div> </div> </div> <div id="ds_animal_free" class="datasheet_section"> <h2>Product Specific Notices</h2> <br>This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354. </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/adult-neurogenesis">Adult Neurogenesis</a> </li> <li> <a href="/research-area/cancer-biomarkers">Cancer Biomarkers</a> </li> <li> <a href="/research-area/cancer-stem-cell-markers">Cancer Stem Cell Markers</a> </li> <li> <a href="/research-area/epigenetics">Epigenetics</a> </li> <li> <a href="/research-area/epigenetics-and-emt">Epigenetics and EMT</a> </li> <li> <a href="/research-area/hematopoietic-stem-cell-markers">Hematopoietic Stem Cell Markers</a> </li> <li> <a href="/research-area/intestinal-stem-cell-markers">Intestinal Stem Cell Markers</a> </li> <li> <a href="/research-area/neural-stem-cell-markers">Neural Stem Cell Markers</a> </li> <li> <a href="/research-area/stem-cell-epigenetics">Stem Cell Epigenetics</a> </li> </ul> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/primary-antibodies"> View all Antibody FAQs </a> </div> </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human BMI-1 Alexa Fluor® 750-conjugated Antibody</h3> <div class="row"> <div class="col-xs-12"> <div> <div class="row"> <div class="col-md-6"> <p>There are currently no reviews for this product. 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