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(PDF) Biocontrol Potential of Plant Growth Promoting Rhizobacterium (PGPR)-Pseudomonas against Plant Pathogenic Fungi | zahoor baba - Academia.edu
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The production of antifungal compounds by the isolated rhizobacteria was assessed against two Foc isolates, coded Foc-S1 and Foc-S2. Strains E1FP13, E1FP4, and E1PP7 were the most effective against Foc-S1, with percentages of 52.77%, 48.37%, and 47.97%, respectively, while E1PP6, E1FP13, and E1PP15 were the most effective against Foc-S2 with percentages of 52.20%, 52.09%, and 45.38%, respectively. All five isolates were identified as Pseudomonas species using 16S rRNA sequencing. The microscopic examination of the impact of the Pseudomonas strains on Foc revealed that all five strains caused morphological changes in Foc, such as granulation and condensation of the cytoplasm, fragmentation, and deformation of the hyphae. 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The research work was carried out with the aim to characterize Pseudomonas sp. biochemically and study biocontrol potential against different plant pathogens i.e. Dematophora sp., Fusarium sp., Pythium sp. and Sclerotium sp. Among the isolates tested, Ar-3-Nag showed maximum % inhibition against Dematophora sp., Fusarium sp. and Sclerotium sp. i.e. 37.5, 34.20 and 45.71 respectively. Against Pythium sp. maximum % inhibition was showed by An-5-Kul i.e. 37.5%. Five isolates i.e. An-4-Kul, An-5-Kul, An-11-Kul, Ar-3-Nag and PN-6-San showed inhibition of mycelial growth against all four fungus pathogens. 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The exploitation of the PGPR in sustainable agriculture as biopesticides / biofertilizers has became a real alternative for agrochemicals used as environment friendly agriculture. In the rhizosphere, Pseudomonas spp. are an important and abundant group affecting plant growth. From diverse agro-ecosystems of Algeria. In this study, 19 fluorescent strains of Pseudomonas spp were isolated from the rhizosphere roots and soils of durum wheat and barley from different biotopes, these strains were screened in vitro for their plant growth promoting activity and the biocontrol of phytopathogenic fungi. From an in vitro bioassay of Lactuca sativa, 8 strains were selected as PGPR. According to phenotypic identification, the strains were affiliated with P.aeruginosa, P.fluorescens and P.putida species. These strains were tested for their ability to produce enzymes of agricultural interest of which OD11, FR14, FR24, FR13 have been producing proteases, amylases, lipases. The 8 isolates were studied for their antagonistic power against Fusarium oxysporum. FR14 and FR15 showed significant inhibition of mycelial growth with a percentage inhibition of 52.72% and 41.81% respectively. Two antifungal metabolites (phenazines, pyrrolnitrins) were extracted from cultures of strains FR14 and FR15 by thin layer chromatography. The R f values obtained ranged from 0.6 to 0.62 for PHZ and absence of PLN. The phenazic extracts were re-tested for their antifungal activities against Fusarium.oxysporum and Aspergillus.niger, the results showed an important inhibition of pathogens by the extract of the strain FR14 with a percentage of inhibition of 11.66% to 23, 63%. These results allow FR14 and FR15 strains to be potential biocontrol agents for a future application. Understanding on the diversity of a most important genus of PGPR in the rhizosphere and mechanism of action should facilitate their application as a reliable component in the management of sustainable agricultural system.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Isolation of fluorescent Pseudomonas spp. strains from rhizosphere agricultural soils and assessment of their role in plant growth and phytopathogen biocontrol","attachmentId":79362440,"attachmentType":"pdf","work_url":"https://www.academia.edu/69160292/Isolation_of_fluorescent_Pseudomonas_spp_strains_from_rhizosphere_agricultural_soils_and_assessment_of_their_role_in_plant_growth_and_phytopathogen_biocontrol","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/69160292/Isolation_of_fluorescent_Pseudomonas_spp_strains_from_rhizosphere_agricultural_soils_and_assessment_of_their_role_in_plant_growth_and_phytopathogen_biocontrol"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="82439634" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/82439634/Potential_of_Rhizospheric_Pseudomonas_Strains_to_Manage_Fusarium_Wilt_of_Tomato">Potential of Rhizospheric Pseudomonas Strains to Manage Fusarium Wilt of Tomato</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="989431" href="https://independent.academia.edu/SabinFatima">Sabin Fatima</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2017</p><p class="ds-related-work--abstract ds2-5-body-sm">Fusarium wilt induced by Fusarium oxysporum f. sp. lycopersici (FOL) has hampered tomato production worldwide. The present research work was conducted at the Institute of Agricultural Sciences, University of Punjab, Qauid-e-Azam Campus, Lahore, Pakistan during the year 2013-14 to search for a biological control strategy to combat this disease. For this purpose indigenous non-pathogenic rhizospheric isolates of Pseudomonas spp. were collected to test their potential to induce resistance in tomato against Fusarium wilt. On preliminary grounds 31 isolates of Pseudomonas spp. were tested for in vitro antibiosis. Eight isolates that efficiently inhibited the growth of F. oxysporum were selected for further experimentation. Greenhouse study revealed that two bacterial isolates i.e. PM12 and PM29 significantly reduced disease index ≥ 60% in two Fusarium wilt susceptible tomato varieties viz: Nagina and RioGrande. In addition these isolates resulted in higher accumulation of defense related...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Potential of Rhizospheric Pseudomonas Strains to Manage Fusarium Wilt of Tomato","attachmentId":88148646,"attachmentType":"pdf","work_url":"https://www.academia.edu/82439634/Potential_of_Rhizospheric_Pseudomonas_Strains_to_Manage_Fusarium_Wilt_of_Tomato","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/82439634/Potential_of_Rhizospheric_Pseudomonas_Strains_to_Manage_Fusarium_Wilt_of_Tomato"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="116258277" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/116258277/Antagonistic_Effect_of_Pseudomonas_spp_on_Pythium_aphanidermatum_and_on_Plant_Growth_of_Chilli_Capsicum_annuum_L_">Antagonistic Effect of Pseudomonas spp on Pythium aphanidermatum and on Plant Growth of Chilli. (Capsicum annuum L.)</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="64053717" href="https://independent.academia.edu/JaneClarynBenjaminjanebenjamin">Jane Claryn Benjamin jane.benjamin</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Current Microbiology and Applied Sciences, 2021</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Antagonistic Effect of Pseudomonas spp on Pythium aphanidermatum and on Plant Growth of Chilli. (Capsicum annuum L.)","attachmentId":112439789,"attachmentType":"pdf","work_url":"https://www.academia.edu/116258277/Antagonistic_Effect_of_Pseudomonas_spp_on_Pythium_aphanidermatum_and_on_Plant_Growth_of_Chilli_Capsicum_annuum_L_","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/116258277/Antagonistic_Effect_of_Pseudomonas_spp_on_Pythium_aphanidermatum_and_on_Plant_Growth_of_Chilli_Capsicum_annuum_L_"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="75953807" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/75953807/Isolation_and_screening_of_Plant_growth_promoting_Pseudomonas_sp_from_phyllosphere_and_rhizosphere">Isolation and screening of Plant growth promoting Pseudomonas sp. from phyllosphere and rhizosphere</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="111791683" href="https://independent.academia.edu/ranjurathour">ranju rathour</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2017</p><p class="ds-related-work--abstract ds2-5-body-sm">Use of chemicals as pesticides and fertilizers has been raised in last few decades which increase the yield upto some extant but also resulted in bioaccumulation in living system. Microbes have the ability to support the plant growth without generating toxic residues and act as biofertilizer. In present work, samples were collected from rhizosphere and phyllosphere of Triticum astivum. After primary screening total 75 isolates of fluorescent Pseudomonas were obtained and further screened for various plant growth promoting (PGP) traits such as Phosphate solubilization, N2 fixation, IAA and biocontrol activity such as siderophore, HCN ammonia production and antifungal activity against selected pathogenic fungi (Fusarium oxysporum, Rhizoctonia sp and Sclerotium sp). Among these isolates, L14, S23 and Y30 were identified as Pseudomonas fluorescence, Pseudomonas putida and Pseudomonas aerofacienss respectively. Results thus obtained from current work were quite promising as per expected ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Isolation and screening of Plant growth promoting Pseudomonas sp. from phyllosphere and rhizosphere","attachmentId":83634169,"attachmentType":"pdf","work_url":"https://www.academia.edu/75953807/Isolation_and_screening_of_Plant_growth_promoting_Pseudomonas_sp_from_phyllosphere_and_rhizosphere","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/75953807/Isolation_and_screening_of_Plant_growth_promoting_Pseudomonas_sp_from_phyllosphere_and_rhizosphere"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="50198999" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/50198999/Applications_of_New_Rhizobacteria_Pseudomonas_Isolates_in_Agroecology_via_Fundamental_Processes_Complementing_Plant_Growth">Applications of New Rhizobacteria Pseudomonas Isolates in Agroecology via Fundamental Processes Complementing Plant Growth</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="15819778" href="https://independent.academia.edu/JamesFurze">James Furze</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Scientific Reports</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Applications of New Rhizobacteria Pseudomonas Isolates in Agroecology via Fundamental Processes Complementing Plant Growth","attachmentId":68274111,"attachmentType":"pdf","work_url":"https://www.academia.edu/50198999/Applications_of_New_Rhizobacteria_Pseudomonas_Isolates_in_Agroecology_via_Fundamental_Processes_Complementing_Plant_Growth","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/50198999/Applications_of_New_Rhizobacteria_Pseudomonas_Isolates_in_Agroecology_via_Fundamental_Processes_Complementing_Plant_Growth"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":113760774,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":113760774,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_113760774" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="38852123" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/38852123/The_bean_rhizosphere_Pseudomonas_aeruginosa_strain_RZ9_strongly_reduces_Fusarium_culmorum_growth_and_infectiveness_of_plant_roots">The bean rhizosphere Pseudomonas aeruginosa strain RZ9 strongly reduces Fusarium culmorum growth and infectiveness of plant roots</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="95690686" href="https://tunis.academia.edu/ImenHaddoudi">Imen Haddoudi</a></div><div 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class="ds-related-work--metadata ds2-5-body-xs">2021</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Pseudomonas as Plant Growth-Promoting Bacteria and Its Role in Alleviation of Abiotic Stress","attachmentId":78293524,"attachmentType":"pdf","work_url":"https://www.academia.edu/67501209/Pseudomonas_as_Plant_Growth_Promoting_Bacteria_and_Its_Role_in_Alleviation_of_Abiotic_Stress","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/67501209/Pseudomonas_as_Plant_Growth_Promoting_Bacteria_and_Its_Role_in_Alleviation_of_Abiotic_Stress"><span class="ds2-5-text-link__content">View 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