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We continue to adopt and pioneer development of cutting-edge sample processing, data collection, and data analysis technologies of greatest importance. Major areas of focus include:" /> <link rel="shortlink" href="https://www.broadinstitute.org/node/8530" /> <link rel="canonical" href="https://www.broadinstitute.org/proteomics" /> <link rel="image_src" href="https://www.broadinstitute.org/files/main_mAD-Figure-1_20221206-01.png" /> <meta property="og:site_name" content="@broadinstitute" /> <meta property="og:type" content="article" /> <meta property="og:url" content="https://www.broadinstitute.org/proteomics" /> <meta property="og:title" content="Proteomics" /> <meta property="og:description" content="The Proteomics Platform provides best-in-class mass spectrometry-based proteomic technologies and analysis capabilities to the Broad community and collaborators throughout the United States and beyond, with the goal of addressing important questions in biology, chemistry, and clinical sciences through intensive collaboration. We continue to adopt and pioneer development of cutting-edge sample processing, data collection, and data analysis technologies of greatest importance. Major areas of focus include:" /> <meta property="og:image" content="https://www.broadinstitute.org/files/main_mAD-Figure-1_20221206-01.png" /> <meta property="og:image:url" content="https://www.broadinstitute.org/files/main_mAD-Figure-1_20221206-01.png" /> <meta property="og:image:secure_url" content="https://www.broadinstitute.org/files/main_mAD-Figure-1_20221206-01.png" /> <meta property="og:updated_time" content="2025-01-27T13:00:28-05:00" /> <meta property="article:published_time" content="2016-05-27T15:36:16-04:00" /> <meta property="article:modified_time" content="2016-05-27T15:36:16-04:00" /> <meta name="twitter:card" content="summary" /> <meta name="twitter:description" content="The Proteomics Platform provides best-in-class mass spectrometry-based proteomic technologies and analysis capabilities to the Broad community and collaborators throughout the United States and beyond, with the goal of addressing important questions in biology, chemistry, and clinical sciences through intensive collaboration. We continue to adopt and pioneer development of cutting-edge sample processing, data collection, and data analysis technologies of greatest importance. Major areas of focus include:" /> <meta name="twitter:site" content="@broadinstitute" /> <meta name="twitter:title" content="Proteomics" /> <meta name="twitter:creator" content="@broadinstitute" /> <meta name="twitter:image" content="https://www.broadinstitute.org/files/main_mAD-Figure-1_20221206-01.png" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <link rel="icon" href="/themes/custom/broad/favicon.ico" type="image/vnd.microsoft.icon" /> <title>Proteomics | Broad Institute</title> <link rel="stylesheet" media="all" 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class="hero-section container"> <div class="hero-section__row row"> <div class="hero-section__content hero-section__content_left col-6"> <div class="hero-section__breadcrumbs"> <div class="block block-system block-system-breadcrumb-block"> <nav class="breadcrumb" role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/technology-areas">Technology Areas</a> </li> </ol> </nav> </div> </div> <div class="hero-section__title"> <div class="block block-layout-builder block-field-blocknodelanding-pagetitle"> <span class="field field--name-title field--type-string field--label-hidden"><h1>Proteomics</h1> </span> </div> </div> <div class="hero-section__description"> <div class="block block-layout-builder block-field-blocknodelanding-pagefield-featured-description"> <div class="clearfix text-formatted field field--name-field-featured-description field--type-text-long field--label-hidden field__item"><p dir="ltr">The Broad’s Proteomics Platform develops and applies advanced proteomics methods to further our understanding of disease, pathways, targets, and drug effects.</p> </div> </div> </div> </div> <div class="hero-section__right col-6"> <div class="hero-section__image"> <div class="block block-layout-builder block-field-blocknodelanding-pagefield-image"> <div class="field field--name-field-image field--type-entity-reference field--label-hidden field__item"> <article class="media media--type-image media--view-mode-multiple-content-types-header"> <div class="field field--name-field-media-image field--type-image field--label-hidden field__item"> <picture> <source srcset="/files/styles/multiple_ct_header_desktop_xl/public/main_mAD-Figure-1_20221206-01.png?h=93c99ee8&itok=LW0HS-wD 1x" media="all and (min-width: 1921px)" type="image/png" width="754" height="503"/> <source srcset="/files/styles/multiple_ct_header_desktop_xl/public/main_mAD-Figure-1_20221206-01.png?h=93c99ee8&itok=LW0HS-wD 1x" media="all and (min-width: 1601px) and (max-width: 1920px)" type="image/png" width="754" height="503"/> <source srcset="/files/styles/multiple_ct_header_desktop/public/main_mAD-Figure-1_20221206-01.png?h=93c99ee8&itok=xJuQxyDY 1x" media="all and (min-width: 1340px) and (max-width: 1600px)" type="image/png" width="736" height="520"/> <source srcset="/files/styles/multiple_ct_header_laptop/public/main_mAD-Figure-1_20221206-01.png?h=93c99ee8&itok=aJPP_cWW 1x" media="all and (min-width: 800px) and (max-width: 1339px)" type="image/png" width="641" height="451"/> <source srcset="/files/styles/multiple_ct_header_tablet/public/main_mAD-Figure-1_20221206-01.png?h=93c99ee8&itok=_6B0CO5e 1x" media="all and (min-width: 540px) and (max-width: 799px)" type="image/png" width="706" height="417"/> <source srcset="/files/styles/multiple_ct_header_phone/public/main_mAD-Figure-1_20221206-01.png?h=93c99ee8&itok=g8LY84Wx 1x" media="all and (max-width: 539px)" type="image/png" width="499" height="294"/> <img loading="eager" width="499" height="294" src="/files/styles/multiple_ct_header_phone/public/main_mAD-Figure-1_20221206-01.png?h=93c99ee8&itok=g8LY84Wx" alt="Fluorescence microscopy image of brain tissue from a mouse model of Alzheimer’s, showing tau tangles (red), amyloid plaques (white), RNA (green), and cell nuclei (blue)." title="Fluorescence microscopy image of brain tissue from a mouse model of Alzheimer’s, showing tau tangles (red), amyloid plaques (white), RNA (green), and cell nuclei (blue)." typeof="foaf:Image" /> </picture> </div> <div class="media-caption"> <div class="media-caption__description"> </div> </div> </article> </div> </div> </div> </div> </div> </div> <div class="content-section content-section_with-sidebars container"> <div class="row"> <div class="content-section__main col-8 offset-2"> <div class="block block-layout-builder block-field-blocknodelanding-pagebody"> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><p>The Proteomics Platform provides best-in-class mass spectrometry-based proteomic technologies and analysis capabilities to the Broad community and collaborators throughout the United States and beyond, with the goal of addressing important questions in biology, chemistry, and clinical sciences through intensive collaboration. We continue to adopt and pioneer development of cutting-edge sample processing, data collection, and data analysis technologies of greatest importance. </p> <p>Major areas of focus include:</p> <ul> <li>Multi -omic and proteogenomic studies of a wide range of cancers, metabolic, and infectious diseases </li> <li>Define cellular signaling networks related to protein phosphorylation, ubiquitylation, acetylation, and cross-talk</li> <li>Identify targets of drugs or identifying protein interactors and interaction surfaces through chemical proteomics technologies, affinity purification mass spectrometry (MS) and hydrogen/deuterium exchange MS</li> <li>Develop biological understanding of response and resistance to therapy </li> <li>Improve understanding of immune biology and help develop personalized immunotherapies through analysis of HLA class I and II peptides</li> <li>Single cell and spatial tissue proteomics</li> <li>Plasma and CSF proteomics </li> <li>Invent and optimize proteomic technologies, automation methods, and computational analyses to increase speed, sensitivity, and completeness of proteome and post-translational modification data, and to improve the ability to extract new knowledge therefrom</li> </ul> <p>Together, these developments have enabled us to apply for and participate in numerous grant opportunities that leverage our novel capabilities, capacity, and throughput to provide a systems-wide and systematic approach to biology and necessary follow-up to genomic discoveries.</p> <p>We wish to acknowledge ongoing support via grants from the National Institutes of Health, the National Cancer Institute, and the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation among others.</p> </div> </div> </div> <div class="content-section__right col-2"> <div class="open block block-broad-menu-reference block-broad-menu-blockadmin"> <h2>Proteomics</h2> <ul class="menu"> <li class="menu-item"> <a href="/proteomics" data-drupal-link-system-path="node/8530" class="is-active" aria-current="page">About</a> </li> <li class="menu-item"> <a href="/proteomics/contact" data-drupal-link-system-path="node/8738">Contact</a> </li> <li class="menu-item"> <a href="/proteomics/collaborating-proteomics-platform" data-drupal-link-system-path="node/2578">How to Establish a Collaboration</a> </li> <li class="menu-item menu-item--expanded"> <div class="dropdown-reference"> <a href="/proteomics/proteomics-team" data-drupal-link-system-path="node/8575">People</a> <div class="dropdown-reference-btn"></div> </div> <ul class="menu"> <li class="menu-item"> <a href="/proteomics/proteomics-team" data-drupal-link-system-path="node/8575">Current</a> </li> <li class="menu-item"> <a href="/proteomics/proteomics-alumni" data-drupal-link-system-path="node/684496">Alumni</a> </li> </ul> </li> <li class="menu-item"> <a href="/proteomics/proteomics-facility" data-drupal-link-system-path="node/9211">Proteomics Facility</a> </li> <li class="menu-item"> <a href="/publications-list/585" target="_blank" data-drupal-link-system-path="publications-list/585">Recent Publications</a> </li> <li class="menu-item menu-item--expanded"> <div class="dropdown-reference"> <a href="/proteomics/protocols" data-drupal-link-system-path="node/747201">Resources</a> <div class="dropdown-reference-btn"></div> </div> <ul class="menu"> <li class="menu-item"> <a href="/datasets" data-drupal-link-system-path="node/1037846">Datasets</a> </li> <li class="menu-item"> <a href="/proteomics/protocols" data-drupal-link-system-path="node/747201">Protocols</a> </li> <li class="menu-item"> <a href="/proteomics/tutorials-and-workshops" data-drupal-link-system-path="node/2606">Tutorials and Workshops</a> </li> </ul> </li> </ul> </div> <div class="block block-ctools block-entity-viewnode"> <article about="/proteomics" class="node node--type-landing-page node--view-mode-sidebar"> <div class="node__content"> </div> </article> </div> </div> </div> </div> <div class="content-section container"> <div class="content-section__main"> <div class="views-element-container block block-views block-views-blockrelated-publications-list-more-link"> <div class="publications-list-wrapper"> <div class="publications-list-wrapper__title"> <h2>Recent publications</h2> <div class="more-link"> <a href="/publications-list/585">Other Proteomics Platform publications</a> </div> </div> <div><div class="view view-related-publications view-id-related_publications view-display-id-list_more_link js-view-dom-id-3328fcfdc13133d465396beacd358544c8ed4f60632676bc3530d99046ed60ac"> <div class="view-content"> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a href="/publications/broad1353501" hreflang="en">Cell-surface Milieu Remodeling in Human Dendritic Cell Activation.</a></span></div></div> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a href="/publications/broad1353901" hreflang="en">Systematic identification of minor histocompatibility antigens predicts outcomes of allogeneic hematopoietic cell transplantation.</a></span></div></div> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a href="/publications/broad1355126" hreflang="en">Molecular basis of TMED9 oligomerization and entrapment of misfolded protein cargo in the early secretory pathway.</a></span></div></div> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a href="/publications/broad1355826" hreflang="en">High-quality peptide evidence for annotating non-canonical open reading frames as human proteins.</a></span></div></div> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a href="/publications/broad1344046" hreflang="en">Protein interaction networks in the vasculature prioritize genes and pathways underlying coronary artery disease.</a></span></div></div> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a href="/publications/broad1357381" hreflang="en">Directed evolution of the multicopper oxidase laccase for cell surface proximity labeling and electron microscopy.</a></span></div></div> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a href="/publications/broad1357386" hreflang="en">A case study to engage students in evolutionary thinking around antibiotic resistance using the MEGA-plate experiment.</a></span></div></div> <div class="views-row"><div class="views-field views-field-title"><span class="field-content"><a 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