Molecular Characterization of Echinococcus granulosus through Amplification of 12S rRNA Gene and Cox1 Gene Fragments from Cattle in Chittagong, Bangladesh

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Echinococcus granulosus through Amplification of 12S rRNA Gene and Cox1 Gene Fragments from Cattle in Chittagong, Bangladesh</title> <meta name="description" content="Molecular Characterization of Echinococcus granulosus through Amplification of 12S rRNA Gene and Cox1 Gene Fragments from Cattle in Chittagong, Bangladesh"> <meta name="keywords" content="Echinococcus granulosus, Cox1, 12S rRNA, molecular characterization, Bangladesh"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div 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class="card"> <div class="card-body"><strong>Paper Count:</strong> 87409</div> </div> </div> </div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Molecular Characterization of Echinococcus granulosus through Amplification of 12S rRNA Gene and Cox1 Gene Fragments from Cattle in Chittagong, Bangladesh</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Omer%20Faruk">M. Omer Faruk</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20M.%20A.%20M.%20Zonaed%20Siddiki"> A. M. A. M. Zonaed Siddiki</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Fazal%20Karim"> M. Fazal Karim</a>, <a href="https://publications.waset.org/abstracts/search?q=Md.%20Masuduzzaman"> Md. Masuduzzaman</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Chowdhury"> S. Chowdhury</a>, <a href="https://publications.waset.org/abstracts/search?q=Md.%20Shafiqul%20Islam"> Md. Shafiqul Islam</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Alamgir%20Hossain"> M. Alamgir Hossain</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The dog tapeworms <em>Echinococcus granulosus</em> develop hydatid cysts in various organs in human and domestic animals worldwide including Bangladesh. The aim of this study was to identify and characterize the genotype of <em>E. granulosus</em> isolated from cattle using 12S rRNA and Cytochrome oxidase 1 (COX 1) genes. A total of 43 hydatid cyst samples were collected from 390 examined cattle samples derived from slaughterhouses. Among them, three cysts were fertile. Genomic DNA was extracted from germinal membrane and/or protoscoleces followed by PCR amplification of mitochondrial 12S rRNA and Cytochrome oxidase 1 gene fragments. The sequence data revealed existence of G1 (64.28%) and possible G3 (21.43%) genotypes for the first time in Bangladesh. The study indicates that common sheep strain G1 is the dominant subtype of <em>E. granulosus</em> in Chittagong region of Bangladesh. This will increase our understanding of the epidemiology of hydatidosis in the southern part of the country and will be useful to plan suitable control measures in the long run. <iframe src="https://publications.waset.org/abstracts/59060.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Echinococcus%20granulosus" title="Echinococcus granulosus">Echinococcus granulosus</a>, <a href="https://publications.waset.org/abstracts/search?q=Cox1" title=" Cox1"> Cox1</a>, <a href="https://publications.waset.org/abstracts/search?q=12S%20rRNA" title=" 12S rRNA"> 12S rRNA</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20characterization" title=" molecular characterization"> molecular characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=Bangladesh" title=" Bangladesh"> Bangladesh</a> </p> <a href="https://publications.waset.org/abstracts/59060/molecular-characterization-of-echinococcus-granulosus-through-amplification-of-12s-rrna-gene-and-cox1-gene-fragments-from-cattle-in-chittagong-bangladesh" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59060.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">344</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET 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Molecular Characterization of Echinococcus granulosus through Amplification of 12S rRNA Gene and Cox1 Gene Fragments from Cattle in Chittagong, Bangladesh