CINXE.COM

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class="ds_title">Recombinant Human SMOC-2 Protein, CF </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: 5140-SM</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 4 Citations </a> </span> <a href="/reviews/5140-SM" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/recombinant-human-smoc-2-protein-cf_5140-sm" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog 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overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="R&D Systems Recombinant Proteins and Enzymes" data-footer="R&D Systems Recombinant Proteins and Enzymes" title="Cat. No. 5140-SM - Recombinant Human SMOC-2 Protein, CF" href="https://resources.rndsystems.com/categoryimages/rndsystems_recombinant-proteins-enzymes.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/categoryimages/rndsystems_recombinant-proteins-enzymes.jpg" class="img-fluid" alt="R&D Systems Recombinant Proteins and Enzymes" title="R&D Systems Recombinant Proteins and Enzymes"> </a> <div class="search_image_other_count"> <span class="search_more_images">1 Image</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/smoc-2?category=Proteins and Enzymes" class="btn btn-secondary">All SMOC-2 Proteins and Enzymes</a> </div> </div> <div class="tabbed_content" id="rnd_protein"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (4)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_carrier_free" id="product-anchor-ds_carrier_free">Carrier Free</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Images</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_calculator" id="product-anchor-ds_calculator">Reconstitution Calculator</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <div id="ds_summary" class="datasheet_section"> <h2>Recombinant Human SMOC-2 Protein, CF Summary</h2> <div class="what-statement-container"> </div> <div class="why-statement-container"> </div> <h4 class="mt-3">Product Specifications</h4> <div class="ds_table summary_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Purity</div> <div class="ds_table_cell summary_table_data">>90%, by SDS-PAGE under reducing conditions and visualized by silver stain</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Endotoxin Level</div> <div class="ds_table_cell summary_table_data"><0.01 EU per 1 μg of the protein by the LAL method.</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Activity</div> <div class="ds_table_cell summary_table_data">Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. <P style="MARGIN: 0in 0in 0pt" class=MsoNormal>When 5 x 10<SUP>4</SUP> cells/well are added to rhSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED<SUB>50 </SUB>for this effect is 0.4‑1.6 μg/mL.<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></P></div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Source</div> <div class="ds_table_cell summary_table_data">Chinese Hamster Ovary cell line, CHO-derived human SMOC-2 protein<br />Gln22-Gly446, with a C-terminal 6-His tag</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Accession #</div> <div class="ds_table_cell summary_table_data"><a href="http://www.ncbi.nlm.nih.gov/protein/AAH47583" target="_blank" rel="noreferrer nofollow">AAH47583</a></div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">N-terminal Sequence<br />Analysis</div> <div class="ds_table_cell summary_table_data">No results obtained: Gln22 predicted<br></div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Predicted Molecular Mass</div> <div class="ds_table_cell summary_table_data">48.3 kDa</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">SDS-PAGE</div> <div class="ds_table_cell summary_table_data">58-66 kDa, reducing conditions</div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="5140-SM"></div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <h2>5140-SM </h2> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/5140-sm.pdf?v=20241126" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/recombinant-human-smoc-2-protein-cf_5140-sm">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/5140-sm?v=20241126">SDS</a> </div> </div> </div> <div id="ds_carrier_free" class="datasheet_section"> <h2>Carrier Free</h2> <div class="highlight">What does CF mean?</div> <p>CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. </p> <div class="highlight">What formulation is right for me?</div> <p>In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere. </p> <div class="cf_tables row"> <div class="cf_table_1 col-xs-12"> <h4 class="cf_table_name">5140-SM</h4> <table class="ds_table_html carrier_free_table"> <tbody> <tr> <td class="cf_key_name">Formulation</td> <td>Lyophilized from a 0.2 μm filtered solution in PBS.</td> </tr> <tr> <td class="cf_key_name">Reconstitution</td> <td>Reconstitute at 200 μg/mL in PBS.</td> </tr> <tr> <td class="cf_key_name">Shipping</td> <td>The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.</td> </tr> <tr> <td class="cf_key_name">Stability & Storage:</td> <td><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles.</b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>3 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL></td> </tr> </tbody> </table> </div> </div> </div> <div id="ds_calculator" class="datasheet_section"> <div id="btn_calculator_wrapper"><div class="datasheet_calculator_toggle btn btn-secondary" id="btn_reconstitution">Reconstitution Calculator</div></div><div id="datasheet_calculators"><div id="btn_reconstitution_calc" class="datasheet_calculator"><h2>Reconstitution Calculator</h2><div id="reconstitution_calculator" class="calculator"> <form id="recCalc"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume (to add to vial)</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </span> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass (in vial)</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </span> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Desired Reconstitution Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="conc." class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </span> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button btn btn-secondary"/> </div> </form> </div> </div></div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: SMOC-2</h2> <p><P>SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins (1, 2). The human SMOC-2 cDNA encodes 446 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40 - 84), two thyroglobulin type-1 segments (aa 87 - 153 and 213 - 281) and two EF-hand sequences (aa 347 - 382 and 384 - 419) (1, 2). Of three splice variants, one shows a 13 aa substitution for aa 442 - 446, another shows an 11 aa insertion after Thr170, and a third contains both of these variations. Mature human SMOC-2 shares 95%, 95% and 94% aa identity with mouse, rat and canine SMOC-2, respectively. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions (1 - 5). Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alpha v beta 1 and alpha v beta 6 (3, 6). SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1 (4). When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis (5). SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle (2). In the skin, it promotes keratinocyte attachment and migration (6). Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery (2, 7).</P></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name"><a id="reftop"></a>References</div> <div class="ds_table_cell background_table_data"><OL> <LI>Vannahme, C. <EM>et al.</EM> (2003) Biochem. J. <STRONG>373</STRONG>:805.</LI> <LI>Nishimoto, S. <EM>et al.</EM> (2002) Biochim. Biophys. Acta <STRONG>1576</STRONG>:225.</LI> <LI>Novinec, M. <EM>et al.</EM> (2008) Protein Expr. Purif. <STRONG>62</STRONG>:75.</LI> <LI>Liu, P. <EM>et al.</EM> (2008) Mol. Biol. Cell <STRONG>19</STRONG>:248.</LI> <LI>Rocnik, E.F. <EM>et al.</EM> (2006) J. Biol. Chem. <STRONG>281</STRONG>:22855.</LI> <LI>Maier, S. <EM>et al.</EM> (2008) Exp. Cell Res. <STRONG>314</STRONG>:2477.</LI> <LI>Wilk, J.B. <EM>et al.</EM> (2007) Am. J. Respir. Crit. Care Med. <STRONG>175</STRONG>:554.</LI></OL></div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name</div> <div class="ds_table_cell background_table_data">Secreted Modular Calcium-binding Protein 2</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs</div> <div class="ds_table_cell background_table_data">64094 (Human); 64074 (Mouse); 292401 (Rat)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names</div> <div class="ds_table_cell background_table_data">bA270C4A.1; bA37D8.1; dJ421D16.1; MST117; MSTP117; MSTP140; SMAP2; SMAP-2; SMAP2Secreted modular calcium-binding protein 2; SMOC2; SMOC-2; smooth muscle associated protein 2; Smooth muscle-associated protein 2; SPARC related modular calcium binding 2; SPARC-related modular calcium-binding protein 2; thyroglobulin type-1 repeat containing protein</div> </div> </div> </div> </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/calcium--binding-proteins-and-related-molecules">Calcium-binding Proteins and Related Molecules</a> </li> <li> <a href="/research-area/extracellular-matrix-molecules">Extracellular Matrix Molecules</a> </li> <li> <a href="/research-area/focal-adhesions">Focal Adhesions</a> </li> <li> <a href="/research-area/osteoblast-and-osteoclast-markers">Osteoblast and Osteoclast Markers</a> </li> </ul> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Recombinant Human SMOC-2 Protein, CF</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">4</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">4</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Canine"> Canine </option> <option class="citation_filterRadio" value="species" data-filter="Human"> Human </option> <option class="citation_filterRadio" value="species" data-filter="Mouse"> Mouse </option> </select> </div> <div class="select_wrapper filter_select"> <select id="selAntibody" title="Applications"> <option value="All">All Applications</option> <option class="citation_filterRadio" value="application" data-filter="Bioassay"> Bioassay </option> <option class="citation_filterRadio" value="application" data-filter="Surface Plasmon Resonance"> Surface Plasmon Resonance </option> </select> </div> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Recombinant Protein"> Recombinant Protein </option> <option class="citation_filterRadio" value="sampletype" data-filter="Whole Cells"> Whole Cells </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="4" data-max-show="10"> <li class="top-lined" data-species="Canine, Human" data-application="Bioassay" data-sampletype="Whole Cells"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35869056"> SMOC2 promotes an epithelial-mesenchymal transition and a pro-metastatic phenotype in epithelial cells of renal cell carcinoma origin </a> </span><br/> Authors: D Feng, P Gao, N Henley, M Dubuissez, N Chen, LP Laurin, V Royal, V Pichette, C Gerarduzzi<br/> Cell Death & Disease, 2022-07-22;13(7):639.<br/> Species: Canine, Human<br/> Sample Types: Whole Cells<br/> Applications: Bioassay </li> <li class="top-lined" data-species="Human" data-application="Bioassay" data-sampletype="Whole Cells"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/32884102"> SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancers </a> </span><br/> Authors: BG Jang, HS Kim, JM Bae, WH Kim, HU Kim, GH Kang<br/> Sci Rep, 2020-09-03;10(1):14591.<br/> Species: Human<br/> Sample Types: Whole Cells<br/> Applications: Bioassay </li> <li class="top-lined" data-species="Mouse" data-application="Bioassay" data-sampletype="Whole Cells"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/27101391"> SMOC Binds to Pro-EGF, but Does Not Induce Erk Phosphorylation via the EGFR </a> </span><br/> Authors: JT Thomas, L Chhuy-Hy, KR Andrykovic, M Moos<br/> PLoS ONE, 2016-04-21;11(4):e0154294.<br/> Species: Mouse<br/> Sample Types: Whole Cells<br/> Applications: Bioassay </li> <li class="top-lined" data-species="Human" data-application="Surface Plasmon Resonance" data-sampletype="Recombinant Protein"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/21349332"> SMOC1 is a tenascin-C interacting protein over-expressed in brain tumors. </a> </span><br/> Authors: Brellier F, Ruggiero S, Zwolanek D, Martina E, Hess D, Brown-Luedi M, Hartmann U, Koch M, Merlo A, Lino M, Chiquet-Ehrismann R<br/> Matrix Biol., 2011-02-21;30(3):225-33.<br/> Species: Human<br/> Sample Types: Recombinant Protein<br/> Applications: Surface Plasmon Resonance </li> </ol> </div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/proteins-and-enzymes"> View all Proteins and Enzyme FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="5140-sm" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for 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