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matrix-assisted laser desorption ionization-time of flight mass spectrometry</title> <meta name="description" content="Search results for: matrix-assisted laser desorption ionization-time of flight mass spectrometry"> <meta name="keywords" content="matrix-assisted laser desorption ionization-time of flight mass spectrometry"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> 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<div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 4965</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: matrix-assisted laser desorption ionization-time of flight mass spectrometry</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4965</span> Body Fluids Identification by Raman Spectroscopy and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Huixia%20Shi">Huixia Shi</a>, <a href="https://publications.waset.org/abstracts/search?q=Can%20Hu"> Can Hu</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun%20Zhu"> Jun Zhu</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongling%20Guo"> Hongling Guo</a>, <a href="https://publications.waset.org/abstracts/search?q=Haiyan%20Li"> Haiyan Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongyan%20Du"> Hongyan Du</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The identification of human body fluids during forensic investigations is a critical step to determine key details, and present strong evidence to testify criminal in a case. With the popularity of DNA and improved detection technology, the potential question must be revolved that whether the suspect’s DNA derived from saliva or semen, menstrual or peripheral blood, how to identify the red substance or aged blood traces on the spot is blood; How to determine who contribute the right one in mixed stains. In recent years, molecular approaches have been developing increasingly on mRNA, miRNA, DNA methylation and microbial markers, but appear expensive, time-consuming, and destructive disadvantages. Physicochemical methods are utilized frequently such us scanning electron microscopy/energy spectroscopy and X-ray fluorescence and so on, but results only showing one or two characteristics of body fluid itself and that out of working in unknown or mixed body fluid stains. This paper focuses on using chemistry methods Raman spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to discriminate species of peripheral blood, menstrual blood, semen, saliva, vaginal secretions, urine or sweat. Firstly, non-destructive, confirmatory, convenient and fast Raman spectroscopy method combined with more accurate matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method can totally distinguish one from other body fluids. Secondly, 11 spectral signatures and specific metabolic molecules have been obtained by analysis results after 70 samples detected. Thirdly, Raman results showed peripheral and menstrual blood, saliva and vaginal have highly similar spectroscopic features. Advanced statistical analysis of the multiple Raman spectra must be requested to classify one to another. On the other hand, it seems that the lactic acid can differentiate peripheral and menstrual blood detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, but that is not a specific metabolic molecule, more sensitivity ones will be analyzed in a forward study. These results demonstrate the great potential of the developed chemistry methods for forensic applications, although more work is needed for method validation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=body%20fluids" title="body fluids">body fluids</a>, <a href="https://publications.waset.org/abstracts/search?q=identification" title=" identification"> identification</a>, <a href="https://publications.waset.org/abstracts/search?q=Raman%20spectroscopy" title=" Raman spectroscopy"> Raman spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix-assisted%20laser%20desorption%2Fionization%20time-of-flight%20mass%20spectrometry" title=" matrix-assisted laser desorption/ionization time-of-flight mass spectrometry"> matrix-assisted laser desorption/ionization time-of-flight mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/103873/body-fluids-identification-by-raman-spectroscopy-and-matrix-assisted-laser-desorptionionization-time-of-flight-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/103873.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">137</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4964</span> A Plasmonic Mass Spectrometry Approach for Detection of Small Nutrients and Toxins</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Haiyang%20Su">Haiyang Su</a>, <a href="https://publications.waset.org/abstracts/search?q=Kun%20Qian"> Kun Qian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We developed a novel plasmonic matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) approach to detect small nutrients and toxin in complex biological emulsion samples. We used silver nanoshells (SiO₂@Ag) with optimized structures as matrices and achieved direct analysis of ~6 nL of human breast milk without any enrichment or separation. We performed identification and quantitation of small nutrients and toxins with limit-of-detection down to 0.4 pmol (for melamine) and reaction time shortened to minutes, superior to the conventional biochemical methods currently in use. Our approach contributed to the near-future application of MALDI MS in a broad field and personalized design of plasmonic materials for real case bio-analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plasmonic%20materials" title="plasmonic materials">plasmonic materials</a>, <a href="https://publications.waset.org/abstracts/search?q=laser%20desorption%2Fionization" title=" laser desorption/ionization"> laser desorption/ionization</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=small%20nutrients" title=" small nutrients"> small nutrients</a>, <a href="https://publications.waset.org/abstracts/search?q=toxins" title=" toxins"> toxins</a> </p> <a href="https://publications.waset.org/abstracts/90310/a-plasmonic-mass-spectrometry-approach-for-detection-of-small-nutrients-and-toxins" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90310.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">211</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4963</span> Rapid Identification of Thermophilic Campylobacter Species from Retail Poultry Meat Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Graziella%20Ziino">Graziella Ziino</a>, <a href="https://publications.waset.org/abstracts/search?q=Filippo%20Giarratana"> Filippo Giarratana</a>, <a href="https://publications.waset.org/abstracts/search?q=Stefania%20Maria%20Marotta"> Stefania Maria Marotta</a>, <a href="https://publications.waset.org/abstracts/search?q=Alessandro%20Giuffrida"> Alessandro Giuffrida</a>, <a href="https://publications.waset.org/abstracts/search?q=Antonio%20Panebianco"> Antonio Panebianco</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In Europe, North America and Japan, campylobacteriosis is one of the leading food-borne bacterial illnesses, often related to the consumption of poultry meats and/or by-products. The aim of this study was the evaluation of Campylobacter contamination of poultry meats marketed in Sicily (Italy) using both traditional methods and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). MALDI-TOF MS is considered a promising rapid (less than 1 hour) identification method for food borne pathogens bacteria. One hundred chicken and turkey meat preparations (no. 68 hamburgers, no. 21 raw sausages, no. 4 meatballs and no. 7 meat rolls) were taken from different butcher’s shops and large scale retailers and submitted to detection/enumeration of Campylobacter spp. according to EN ISO 10272-1:2006 and EN ISO 10272-2:2006. Campylobacter spp. was detected with general low counts in 44 samples (44%), of which 30 from large scale retailers and 14 from butcher’s shops. Chicken meats were significantly more contaminated than turkey meats. Among the preparations, Campylobacter spp. was found in 85.71% of meat rolls, 50% of meatballs, 44.12% of hamburgers and 28.57% of raw sausages. A total of 100 strains, 2-3 from each positive samples, were isolated for the identification by phenotypic, biomolecular and MALDI-TOF MS methods. C. jejuni was the predominant strains (63%), followed by C. coli (33%) and C. lari (4%). MALDI-TOF MS correctly identified 98% of the strains at the species level, only 1% of the tested strains were not identified. In the last 1%, a mixture of two different species was mixed in the same sample and MALDI-TOF MS correctly identified at least one of the strains. Considering the importance of rapid identification of pathogens in the food matrix, this method is highly recommended for the identification of suspected colonies of Campylobacteria. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=campylobacter%20spp." title="campylobacter spp.">campylobacter spp.</a>, <a href="https://publications.waset.org/abstracts/search?q=Food%20Microbiology" title=" Food Microbiology"> Food Microbiology</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix-assisted%20laser%20desorption%20ionization-time%20of%20flight%20mass%20spectrometry" title=" matrix-assisted laser desorption ionization-time of flight mass spectrometry"> matrix-assisted laser desorption ionization-time of flight mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=rapid%20microbial%20identification" title=" rapid microbial identification"> rapid microbial identification</a> </p> <a href="https://publications.waset.org/abstracts/71541/rapid-identification-of-thermophilic-campylobacter-species-from-retail-poultry-meat-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/71541.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">292</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4962</span> A Step-by-Step Analytical Protocol For Detecting and Identifying Minor Differences In Like Materials and Polymers Using Pyrolysis -Gas Chromatography/Mass Spectrometry Technique</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Athena%20Nguyen">Athena Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Rojin%20Belganeh"> Rojin Belganeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Detecting and identifying differences in like polymer materials are key factors in failure and deformulation analysis, and reverse engineering. Pyrolysis-GC/MS is an easy solid sample introduction technique which expands the application areas of gas chromatography and mass spectrometry. The Micro furnace pyrolyzer is directly interfaced with the GC injector preventing any potential of cold spot, carryover, and cross contamination. In this presentation, the analysis of the differences in three polystyrene samples is demonstrated. Although the three samples look very similar by Evolve gas analysis (EGA) and Flash pyrolysis, there are indications of small levels of other materials. By performing Thermal desorption-GC/MS, the additive compounds between samples show the differences. EGA, flash pyrolysis, and thermal desorption analysis are the different modes of operations of the micro-furnace pyrolyzer enabling users to perform multiple analytical techniques. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gas%20chromatography%2FMass%20spectrometry" title="Gas chromatography/Mass spectrometry">Gas chromatography/Mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolysis" title=" pyrolysis"> pyrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolyzer" title=" pyrolyzer"> pyrolyzer</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20desorption-GC%2FMS" title=" thermal desorption-GC/MS"> thermal desorption-GC/MS</a> </p> <a href="https://publications.waset.org/abstracts/139716/a-step-by-step-analytical-protocol-for-detecting-and-identifying-minor-differences-in-like-materials-and-polymers-using-pyrolysis-gas-chromatographymass-spectrometry-technique" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139716.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">187</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4961</span> Deformulation and Comparative Analysis of Apparently Similar Polymers Using Multiple Modes of Pyrolysis-Gc/Ms</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Athena%20Nguyen">Athena Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Rojin%20Belganeh"> Rojin Belganeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Detecting and identifying differences in like polymer materials are key factors in deformulation, comparative analysis as well as reverse engineering. Pyrolysis-GC/MS is an easy solid sample introduction technique which expands the application areas of gas chromatography and mass spectrometry. The Micro-furnace pyrolyzer is directly interfaced with the GC injector preventing any potential of cold spot, carryover, and cross contamination. This presentation demonstrates the study of two similar polymers by performing different mode of operations in the same system: Evolve gas analysis (EGA), Flash pyrolysis, Thermal desorption analysis, and Heart-cutting analysis. Unknown polymer materials and their chemical compositions are identified. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography%2Fmass%20spectrometry" title="gas chromatography/mass spectrometry">gas chromatography/mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolysis" title=" pyrolysis"> pyrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolyzer" title=" pyrolyzer"> pyrolyzer</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20desorption-GC%2FMS" title=" thermal desorption-GC/MS"> thermal desorption-GC/MS</a> </p> <a href="https://publications.waset.org/abstracts/139719/deformulation-and-comparative-analysis-of-apparently-similar-polymers-using-multiple-modes-of-pyrolysis-gcms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139719.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">264</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4960</span> In-vitro Metabolic Fingerprinting Using Plasmonic Chips by Laser Desorption/Ionization Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vadanasundari%20Vedarethinam">Vadanasundari Vedarethinam</a>, <a href="https://publications.waset.org/abstracts/search?q=Kun%20Qian"> Kun Qian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The metabolic analysis is more distal over proteomics and genomics engaging in clinics and needs rationally distinct techniques, designed materials, and device for clinical diagnosis. Conventional techniques such as spectroscopic techniques, biochemical analyzers, and electrochemical have been used for metabolic diagnosis. Currently, there are four major challenges including (I) long-term process in sample pretreatment; (II) difficulties in direct metabolic analysis of biosamples due to complexity (III) low molecular weight metabolite detection with accuracy and (IV) construction of diagnostic tools by materials and device-based platforms for real case application in biomedical applications. Development of chips with nanomaterial is promising to address these critical issues. Mass spectroscopy (MS) has displayed high sensitivity and accuracy, throughput, reproducibility, and resolution for molecular analysis. Particularly laser desorption/ ionization mass spectrometry (LDI MS) combined with devices affords desirable speed for mass measurement in seconds and high sensitivity with low cost towards large scale uses. We developed a plasmonic chip for clinical metabolic fingerprinting as a hot carrier in LDI MS by series of chips with gold nanoshells on the surface through controlled particle synthesis, dip-coating, and gold sputtering for mass production. We integrated the optimized chip with microarrays for laboratory automation and nanoscaled experiments, which afforded direct high-performance metabolic fingerprinting by LDI MS using 500 nL of serum, urine, cerebrospinal fluids (CSF) and exosomes. Further, we demonstrated on-chip direct in-vitro metabolic diagnosis of early-stage lung cancer patients using serum and exosomes without any pretreatment or purifications. To our best knowledge, this work initiates a bionanotechnology based platform for advanced metabolic analysis toward large-scale diagnostic use. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plasmonic%20chip" title="plasmonic chip">plasmonic chip</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolic%20fingerprinting" title=" metabolic fingerprinting"> metabolic fingerprinting</a>, <a href="https://publications.waset.org/abstracts/search?q=LDI%20MS" title=" LDI MS"> LDI MS</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro%20diagnostics" title=" in-vitro diagnostics"> in-vitro diagnostics</a> </p> <a href="https://publications.waset.org/abstracts/91975/in-vitro-metabolic-fingerprinting-using-plasmonic-chips-by-laser-desorptionionization-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91975.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">162</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4959</span> Staphylococcus argenteus: An Emerging Subclinical Bovine Mastitis Pathogen in Thailand </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Natapol%20Pumipuntu">Natapol Pumipuntu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Staphylococcus argenteus is the emerging species of S. aureus complex. It was generally misidentified as S. aureus by standard techniques and their features. S. argenteus is possibly emerging in both humans and animals, as well as increasing worldwide distribution. The objective of this study was to differentiate and identify S. argenteus from S. aureus, which has been collected and isolated from milk samples of subclinical bovine mastitis cases in Maha Sarakham province, Northeastern of Thailand. Twenty-one isolates of S. aureus, which confirmed by conventional methods and immune-agglutination method were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and multilocus sequence typing (MLST). The result from MALDI-TOF MS and MLST showed 6 from 42 isolates were confirmed as S. argenteus, and 36 isolates were S. aureus, respectively. This study indicated that the identification and classification method by using MALDI-TOF MS and MLST could accurately differentiate the emerging species, S. argenteus, from S. aureus complex which usually misdiagnosed. In addition, the identification of S. argenteus seems to be very limited despite the fact that it may be the important causative pathogen in bovine mastitis as well as pathogenic bacteria in food and milk. Therefore, it is very necessary for both bovine medicine and veterinary public health to emphasize and recognize this bacterial pathogen as the emerging disease of Staphylococcal bacteria and need further study about S. argenteus infection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Staphylococcus%20argenteus" title="Staphylococcus argenteus">Staphylococcus argenteus</a>, <a href="https://publications.waset.org/abstracts/search?q=subclinical%20bovine%20mastitis" title=" subclinical bovine mastitis"> subclinical bovine mastitis</a>, <a href="https://publications.waset.org/abstracts/search?q=Staphylococcus%20aureus%20complex" title=" Staphylococcus aureus complex"> Staphylococcus aureus complex</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=MLST" title=" MLST"> MLST</a> </p> <a href="https://publications.waset.org/abstracts/108179/staphylococcus-argenteus-an-emerging-subclinical-bovine-mastitis-pathogen-in-thailand" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/108179.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">151</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4958</span> Waste Egg Albumin Derived Small Peptides Stimulate Photosynthetic Electron Transport</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seungwon%20Han">Seungwon Han</a>, <a href="https://publications.waset.org/abstracts/search?q=Sung%20young%20Yoo"> Sung young Yoo</a>, <a href="https://publications.waset.org/abstracts/search?q=Tae%20Wan%20Kim"> Tae Wan Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The objective of this study was to measure the changes in the photochemical response in the leaves of red pepper (Capsium annuum L.) after foliar fertilization of amino acid and small peptides derived from the waste egg. As a nitrogen fertilizer, waste eggs were incubated over one 1week and then degraded as amino acids and small peptides. The smaller peptides less than 20 kDa were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). MALDI-TOF-MS as a rapid analysis method was to show the molecular mass of degraded egg protein. The sequences of peptides were identified as follows; γ-Glu- Cys-γ-Glu-Cys-γ-Glu-Cys)-Ser and γ-Glu-Cys-γ-Glu-Cys-γ-Glu- Cys)-Gly. It was clearly illuminated that the parameters related to quantum yields for PSI electron transport (ΦRE1O, ΨRE1O, δRE1O) and RC/ABS have increased tendency by small peptide application. On the other hand, phenomenological energy fluxes (ABSO/CSM, TRO/CSM, ET2O/CSM, RE1O/CSM, DIO/CSM) have considerably fluctuated with foliar fertilization of small peptides. In conclusion, the small peptides can enhance the photochemical activities from photosystem II to photosystem I. This study was financially supported by RDA Agenda Project PJ 016196012022. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electron%20transport" title="electron transport">electron transport</a>, <a href="https://publications.waset.org/abstracts/search?q=foliar%20fertilization" title=" foliar fertilization"> foliar fertilization</a>, <a href="https://publications.waset.org/abstracts/search?q=small%20peptide" title=" small peptide"> small peptide</a>, <a href="https://publications.waset.org/abstracts/search?q=waste%20egg" title=" waste egg"> waste egg</a> </p> <a href="https://publications.waset.org/abstracts/143256/waste-egg-albumin-derived-small-peptides-stimulate-photosynthetic-electron-transport" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143256.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">167</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4957</span> Production of Neutrons by High Intensity Picosecond Laser Interacting with Thick Solid Target at XingGuangIII</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xi%20Yuan">Xi Yuan</a>, <a href="https://publications.waset.org/abstracts/search?q=Xuebin%20Zhu"> Xuebin Zhu</a>, <a href="https://publications.waset.org/abstracts/search?q=Bojun%20Li"> Bojun Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work describes the experiment to produce high-intensity pulsed neutron beams on XingGuangIII laser facility. The high-intensity laser is utilized to drive protons and deuterons, which hit a thick solid target to produce neutrons. The pulse duration of the laser used in the experiment is about 0.8 ps, and the laser energy is around 100 J. Protons and deuterons are accelerated from a 10-μm-thick deuterated polyethylene (CD₂) foil and diagnosed by a Thomson parabola ion-spectrometer. The energy spectrum of neutrons generated via ⁷Li(d,n) and ⁷Li(p,n) reaction when proton and deuteron beams hit a 5-mm-thick LiF target is measured by a scintillator-based time-of-flight spectrometer. Results from the neuron measurements show that the maximum neutron energy is about 12.5 MeV and the neutron yield is up to 2×10⁹/pulse. The high-intensity pulsed neutron beams demonstrated in this work can provide a valuable neutron source for material research, fast neutron induced fission research, and so on. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=picosecond%20laser%20driven" title="picosecond laser driven">picosecond laser driven</a>, <a href="https://publications.waset.org/abstracts/search?q=fast%20neutron" title=" fast neutron"> fast neutron</a>, <a href="https://publications.waset.org/abstracts/search?q=time-of-flight%20spectrometry" title=" time-of-flight spectrometry"> time-of-flight spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=XinggungIII" title=" XinggungIII"> XinggungIII</a> </p> <a href="https://publications.waset.org/abstracts/92159/production-of-neutrons-by-high-intensity-picosecond-laser-interacting-with-thick-solid-target-at-xingguangiii" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/92159.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">165</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4956</span> Phenotypical and Genotypical Assessment Techniques for Identification of Some Contagious Mastitis Pathogens</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ayman%20El%20Behiry">Ayman El Behiry</a>, <a href="https://publications.waset.org/abstracts/search?q=Rasha%20Nabil%20Zahran"> Rasha Nabil Zahran</a>, <a href="https://publications.waset.org/abstracts/search?q=Reda%20Tarabees"> Reda Tarabees</a>, <a href="https://publications.waset.org/abstracts/search?q=Eman%20Marzouk"> Eman Marzouk</a>, <a href="https://publications.waset.org/abstracts/search?q=Musaad%20Al-Dubaib"> Musaad Al-Dubaib</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mastitis is one of the most economic disease affecting dairy cows worldwide. Its classic diagnosis using bacterial culture and biochemical findings is a difficult and prolonged method. In this research, using of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) permitted identification of different microorganisms with high accuracy and rapidity (only 24 hours for microbial growth and analysis). During the application of MALDI-TOF MS, one hundred twenty strains of Staphylococcus and Streptococcus species isolated from milk of cows affected by clinical and subclinical mastitis were identified, and the results were compared with those obtained by traditional methods as API and VITEK 2 Systems. 37 of totality 39 strains (~95%) of Staphylococcus aureus (S. aureus) were exactly detected by MALDI TOF MS and then confirmed by a nuc-based PCR technique, whereas accurate identification was observed in 100% (50 isolates) of the coagulase negative staphylococci (CNS) and Streptococcus agalactiae (31 isolates). In brief, our results demonstrated that MALDI-TOF MS is a fast and truthful technique which has the capability to replace conventional identification of several bacterial strains usually isolated in clinical laboratories of microbiology. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=identification" title="identification">identification</a>, <a href="https://publications.waset.org/abstracts/search?q=mastitis%20pathogens" title=" mastitis pathogens"> mastitis pathogens</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectral" title=" mass spectral"> mass spectral</a>, <a href="https://publications.waset.org/abstracts/search?q=phenotypical" title=" phenotypical"> phenotypical</a> </p> <a href="https://publications.waset.org/abstracts/8669/phenotypical-and-genotypical-assessment-techniques-for-identification-of-some-contagious-mastitis-pathogens" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/8669.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">333</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4955</span> Inclusion Complexes of Some Imidazoline Drugs with Cucurbit[N]Uril (N=7,8): Preparation, Characterization and Theoretical Calculations</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fakhreldin%20O.%20Suliman">Fakhreldin O. Suliman</a>, <a href="https://publications.waset.org/abstracts/search?q=Alia%20H.%20Al-Battashi"> Alia H. Al-Battashi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work explored the interaction of three different imidazoline drugs, naphazoline nitrate (NPH), oxymetazoline hydrochloride (OXY) and xylometazoline hydrochloride (XYL) with two different synthesized cucurbit[n]urils CB[n], cucurbit[7]uril (CB[7]) and cucuribit[8]uril (CB[8]). Three binary inclusion complexes have been investigated in solution and in the solid state. The solid complexes were obtained by lyophilization, whereas the physical mixtures of guests and hosts at a stoichiometric ratio of 1:1 were obtained for each drug. 1HNMR, electrospray ionization mass spectrometry (ESI-MS), and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was used to study the complexes prepared in aqueous media. The lyophilized solid complexes were characterized by Fourier transform-infrared spectroscopy (FT-IR), powder X-ray diffractometry (PXRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). MS, FT-IR and PXRD experimental results established in this work reveal that NPH, OXY and XYL molecules form stable inclusion complexes with the two hosts. The TGA and DSC confirmed the enhancement of the thermal stability of each drug and the production of a thermally stable solid complex. The 1HNMR has shown that the protons of the guests faced shifting in ppm and broadening of their peaks upon the formation of inclusion complexes with the selected CB[n]. The aromatic protons of the guest exhibited the highest changes in the chemical shifts and shape of the NMR peaks, suggesting their inclusion into the cavity of the CB[n]. The diffusion coefficients (D), developed from the diffusion-controlled NMR Spectroscopy (DOSY) measurements, for the complexation of the selected imidazoline drugs with CB[7] and CB[8], were decreased in the presence of hosts compared to the free guests indicating the formation of the guest-host adduct. Furthermore, we conducted molecular dynamic simulations and quantum mechanics calculations on these complexes. The results of the theoretical study corroborate the experimental findings and have also shed light on the mechanism of inclusion of the guests into the two hosts. This study generates initial data for potential drug delivery or drug formulation systems for these three selected imidazoline drug compounds based on their inclusion into the CB[n] cavities. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cucurbit%5Bn%5Durils" title="cucurbit[n]urils">cucurbit[n]urils</a>, <a href="https://publications.waset.org/abstracts/search?q=imidazoline" title=" imidazoline"> imidazoline</a>, <a href="https://publications.waset.org/abstracts/search?q=inclusion%20complexes" title=" inclusion complexes"> inclusion complexes</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20dynamics" title=" molecular dynamics"> molecular dynamics</a>, <a href="https://publications.waset.org/abstracts/search?q=DFT%20calculations" title=" DFT calculations"> DFT calculations</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/178820/inclusion-complexes-of-some-imidazoline-drugs-with-cucurbitnuril-n78-preparation-characterization-and-theoretical-calculations" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/178820.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">68</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4954</span> Effect of Naphtha on the Composition of a Heavy Crude, in Addition to a Cycle Steam Stimulation Process </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Guerrero">A. Guerrero</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Leon"> A. Leon</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Munoz"> S. Munoz</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Sandoval"> M. Sandoval</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The addition of solvent to cyclic steam stimulation is done in order to reduce the solvent-vapor ratio at late stages of the process, the moment in which this relationship increases significantly. The study of the use of naphtha in addition to the cyclic steam stimulation has been mainly oriented to the effect it achieves on the incremental recovery compared to the application of steam only. However, the effect of naphtha on the reactivity of crude oil components under conditions of cyclic steam stimulation or if its effect is the only dilution has not yet been considered, to author’s best knowledge. The present study aims to evaluate and understand the effect of naphtha and the conditions of cyclic steam stimulation, on the remaining composition of the improved oil, as well as the main mechanisms present in the heavy crude - naphtha interaction. Tests were carried out with the system solvent (naphtha)-oil (12.5° API, 4216 cP @ 40° C)- steam, in a batch micro-reactor, under conditions of cyclic steam stimulation (250-300 °C, 400 psi). The characterization of the samples obtained was carried out by MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) and NMR (Nuclear Magnetic Resonance) techniques. The results indicate that there is a rearrangement of the microstructure of asphaltenes, resulting in a decrease in these and an increase in lighter components such as resins. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=composition%20change" title="composition change">composition change</a>, <a href="https://publications.waset.org/abstracts/search?q=cyclic%20steam%20stimulation" title=" cyclic steam stimulation"> cyclic steam stimulation</a>, <a href="https://publications.waset.org/abstracts/search?q=interaction%20mechanism" title=" interaction mechanism"> interaction mechanism</a>, <a href="https://publications.waset.org/abstracts/search?q=naphtha" title=" naphtha"> naphtha</a> </p> <a href="https://publications.waset.org/abstracts/111468/effect-of-naphtha-on-the-composition-of-a-heavy-crude-in-addition-to-a-cycle-steam-stimulation-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/111468.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4953</span> Characterization of Dota-Girentuximab Conjugates for Radioimmunotherapy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tais%20Basaco">Tais Basaco</a>, <a href="https://publications.waset.org/abstracts/search?q=Stefanie%20Pektor"> Stefanie Pektor</a>, <a href="https://publications.waset.org/abstracts/search?q=Josue%20A.%20Moreno"> Josue A. Moreno</a>, <a href="https://publications.waset.org/abstracts/search?q=Matthias%20Miederer"> Matthias Miederer</a>, <a href="https://publications.waset.org/abstracts/search?q=Andreas%20T%C3%BCrler"> Andreas Türler</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Radiopharmaceuticals based in monoclonal anti-body (mAb) via chemical linkers have become a potential tool in nuclear medicine because of their specificity and the large variability and availability of therapeutic radiometals. It is important to identify the conjugation sites and number of attached chelator to mAb to obtain radioimmunoconjugates with required immunoreactivity and radiostability. Girentuximab antibody (G250) is a potential candidate for radioimmunotherapy of clear cell carcinomas (RCCs) because it is reactive with CAIX antigen, a transmembrane glycoprotein overexpressed on the cell surface of most ( > 90%) (RCCs). G250 was conjugated with the bifunctional chelating agent DOTA (1,4,7,10-Tetraazacyclododecane-N,N’,N’’,N’’’-tetraacetic acid) via a benzyl-thiocyano group as a linker (p-SCN-Bn-DOTA). DOTA-G250 conjugates were analyzed by size exclusion chromatography (SE-HPLC) and by electrophoresis (SDS-PAGE). The potential site-specific conjugation was identified by liquid chromatography–mass spectrometry (LC/MS-MS) and the number of linkers per molecule of mAb was calculated using the molecular weight (MW) measured by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The average number obtained in the conjugates in non-reduced conditions was between 8-10 molecules of DOTA per molecule of mAb. The average number obtained in the conjugates in reduced conditions was between 1-2 and 3-4 molecules of DOTA per molecule of mAb in the light chain (LC) and heavy chain (HC) respectively. Potential DOTA modification sites of the chelator were identified in lysine residues. The biological activity of the conjugates was evaluated by flow cytometry (FACS) using CAIX negative (SKRC-18) and CAIX positive (SKRC-52). The DOTA-G250 conjugates were labelled with 177Lu with a radiochemical yield > 95% reaching specific activities of 12 MBq/µg. The stability in vitro of different types of radioconstructs was analyzed in human serum albumin (HSA). The radiostability of 177Lu-DOTA-G250 at high specific activity was increased by addition of sodium ascorbate after the labelling. The immunoreactivity was evaluated in vitro and in vivo. Binding to CAIX positive cells (SK-RC-52) at different specific activities was higher for conjugates with less DOTA content. Protein dose was optimized in mice with subcutaneously growing SK-RC-52 tumors using different amounts of 177Lu- DOTA-G250. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title="mass spectrometry">mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=monoclonal%20antibody" title=" monoclonal antibody"> monoclonal antibody</a>, <a href="https://publications.waset.org/abstracts/search?q=radiopharmaceuticals" title=" radiopharmaceuticals"> radiopharmaceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=radioimmunotheray" title=" radioimmunotheray"> radioimmunotheray</a>, <a href="https://publications.waset.org/abstracts/search?q=renal%20cancer" title=" renal cancer"> renal cancer</a> </p> <a href="https://publications.waset.org/abstracts/64738/characterization-of-dota-girentuximab-conjugates-for-radioimmunotherapy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/64738.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">307</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4952</span> Fuel Oxidation Reactions: Pathways and Reactive Intermediates Characterization via Synchrotron Photoionization Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Giovanni%20Meloni">Giovanni Meloni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recent results are presented from experiments carried out at the Advanced Light Source (ALS) at the Chemical Dynamics Beamline of Lawrence Berkeley National Laboratory using multiplexed synchrotron photoionization mass spectrometry. The reaction mixture and a buffer gas (He) are introduced through individually calibrated mass flow controllers into a quartz slow flow reactor held at constant pressure and temperature. The gaseous mixture effuses through a 650 μm pinhole into a 1.5 mm skimmer, forming a molecular beam that enters a differentially pumped ionizing chamber. The molecular beam is orthogonally intersected by a tunable synchrotron radiation produced by the ALS in the 8-11 eV energy range. Resultant ions are accelerated, collimated, and focused into an orthogonal time-of-flight mass spectrometer. Reaction species are identified by their mass-to-charge ratios and photoionization (PI) spectra. Comparison of experimental PI spectra with literature and/or simulated curves is routinely done to assure the identity of a given species. With the aid of electronic structure calculations, potential energy surface scans are performed, and Franck-Condon spectral simulations are obtained. Examples of these experiments are discussed, ranging from new intermediates characterization to reaction mechanisms elucidation and biofuels oxidation pathways identification. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title="mass spectrometry">mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=reaction%20intermediates" title=" reaction intermediates"> reaction intermediates</a>, <a href="https://publications.waset.org/abstracts/search?q=synchrotron%20photoionization" title=" synchrotron photoionization"> synchrotron photoionization</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidation%20reactions" title=" oxidation reactions"> oxidation reactions</a> </p> <a href="https://publications.waset.org/abstracts/173937/fuel-oxidation-reactions-pathways-and-reactive-intermediates-characterization-via-synchrotron-photoionization-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/173937.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">73</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4951</span> Drag Reduction of Base Bleed at Various Flight Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Man%20Chul%20Jeong">Man Chul Jeong</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyoung%20Jin%20Lee"> Hyoung Jin Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Sang%20Yoon%20Lee"> Sang Yoon Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Ji%20Hyun%20Park"> Ji Hyun Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Min%20Wook%20Chang"> Min Wook Chang</a>, <a href="https://publications.waset.org/abstracts/search?q=In-Seuck%20Jeung"> In-Seuck Jeung</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study focus on the drag reduction effect of the base bleed at supersonic flow. Base bleed is the method which bleeds the gas on the tail of the flight vehicle and reduces the base drag, which occupies over 50% of the total drag in any flight speed. Thus base bleed can reduce the total drag significantly, and enhance the total flight range. Drag reduction ratio of the base bleed is strongly related to the mass flow rate of the bleeding gas. Thus selecting appropriate mass flow rate is important. However, since the flight vehicle has various flight speed, same mass flow rate of the base bleed can have different drag reduction effect during the flight. Thus, this study investigates the effect of the drag reduction depending on the flight speed by numerical analysis using STAR-CCM+. The analysis model is 155mm diameter projectile with boat-tailed shape base. Angle of the boat-tail is chosen previously for minimum drag coefficient. Numerical analysis is conducted for Mach 2 and Mach 3, with various mass flow rate, or the injection parameter I, of the bleeding gas and the temperature of the bleeding gas, is fixed to 300K. The results showed that I=0.025 has the minimum drag at Mach 2, and I=0.014 has the minimum drag at Mach 3. Thus as the Mach number is higher, the lower mass flow rate of the base bleed has more effect on drag reduction. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=base%20bleed" title="base bleed">base bleed</a>, <a href="https://publications.waset.org/abstracts/search?q=supersonic" title=" supersonic"> supersonic</a>, <a href="https://publications.waset.org/abstracts/search?q=drag%20reduction" title=" drag reduction"> drag reduction</a>, <a href="https://publications.waset.org/abstracts/search?q=recirculation" title=" recirculation"> recirculation</a> </p> <a href="https://publications.waset.org/abstracts/69358/drag-reduction-of-base-bleed-at-various-flight-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/69358.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">415</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4950</span> Thermal Technologies Applications for Soil Remediation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20de%20Folly%20d%E2%80%99Auris">A. de Folly d’Auris</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Bagatin"> R. Bagatin</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Filtri"> P. Filtri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper discusses the importance of having a good initial characterization of soil samples when thermal desorption has to be applied to polluted soils for the removal of contaminants. Particular attention has to be devoted on the desorption kinetics of the samples to identify the gases evolved during the heating, and contaminant degradation pathways. In this study, two samples coming from different points of the same contaminated site were considered. The samples are much different from each other. Moreover, the presence of high initial quantity of heavy hydrocarbons strongly affected the performance of thermal desorption, resulting in formation of dangerous intermediates. Analytical techniques such TGA (Thermogravimetric Analysis), DSC (Differential Scanning Calorimetry) and GC-MS (Gas Chromatography-Mass) provided a good support to give correct indication for field application. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=desorption%20kinetics" title="desorption kinetics">desorption kinetics</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrocarbons" title=" hydrocarbons"> hydrocarbons</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20desorption" title=" thermal desorption"> thermal desorption</a>, <a href="https://publications.waset.org/abstracts/search?q=thermogravimetric%20measurements" title=" thermogravimetric measurements"> thermogravimetric measurements</a> </p> <a href="https://publications.waset.org/abstracts/40004/thermal-technologies-applications-for-soil-remediation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40004.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">294</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4949</span> A Proteomic Approach for Discovery of Microbial Cellulolytic Enzymes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20S.%20Matlala">M. S. Matlala</a>, <a href="https://publications.waset.org/abstracts/search?q=I.%20Ignatious"> I. Ignatious</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Environmental sustainability has taken the center stage in human life all over the world. Energy is the most essential component of our life. The conventional sources of energy are non-renewable and have a detrimental environmental impact. Therefore, there is a need to move from conventional to non-conventional renewable energy sources to satisfy the world’s energy demands. The study aimed at screening for microbial cellulolytic enzymes using a proteomic approach. The objectives were to screen for microbial cellulases with high specific activity and separate the cellulolytic enzymes using a combination of zymography and two-dimensional (2-D) gel electrophoresis followed by tryptic digestion, Matrix-assisted Laser Desorption Ionisation-Time of Flight (MALDI-TOF) and bioinformatics analysis. Fungal and bacterial isolates were cultured in M9 minimal and Mandel media for a period of 168 hours at 60°C and 30°C with cellobiose and Avicel as carbon sources. Microbial cells were separated from supernatants through centrifugation, and the crude enzyme from the cultures was used for the determination of cellulase activity, zymography, SDS-PAGE, and two-dimensional gel electrophoresis. Five isolates, with lytic action on carbon sources studied, were a bacterial strain (BARK) and fungal strains (VCFF1, VCFF14, VCFF17, and VCFF18). Peak cellulase production by the selected isolates was found to be 3.8U/ml, 2.09U/ml, 3.38U/ml, 3.18U/ml, and 1.95U/ml, respectively. Two-dimensional gel protein maps resulted in the separation and quantitative expression of different proteins by the microbial isolates. MALDI-TOF analysis and database search showed that the expressed proteins in this study closely relate to different glycoside hydrolases produced by other microbial species with an acceptable confidence level of 100%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cellulases" title="cellulases">cellulases</a>, <a href="https://publications.waset.org/abstracts/search?q=energy" title=" energy"> energy</a>, <a href="https://publications.waset.org/abstracts/search?q=two-dimensional%20gel%20electrophoresis" title=" two-dimensional gel electrophoresis"> two-dimensional gel electrophoresis</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix-assisted%20laser%20desorption%20ionisation-time%20of%20flight" title=" matrix-assisted laser desorption ionisation-time of flight"> matrix-assisted laser desorption ionisation-time of flight</a>, <a href="https://publications.waset.org/abstracts/search?q=MALDI-TOF%20MS" title=" MALDI-TOF MS"> MALDI-TOF MS</a> </p> <a href="https://publications.waset.org/abstracts/111097/a-proteomic-approach-for-discovery-of-microbial-cellulolytic-enzymes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/111097.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">134</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4948</span> One Health Approach: The Importance of Improving the Identification of Waterborne Bacteria in Austrian Water</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aurora%20Gitto">Aurora Gitto</a>, <a href="https://publications.waset.org/abstracts/search?q=Philipp%20Proksch"> Philipp Proksch</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The presence of various microorganisms (bacteria, fungi) in surface water and groundwater represents an important issue for human health worldwide. The matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has emerged as a promising and reliable tool for bacteria identification in clinical diagnostic microbiology and environmental strains thanks to an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. The study aims first to conceptualise and set up library information and create a comprehensive database of MALDI-TOF-MS spectra from environmental water samples. The samples were analysed over a year (2021-2022) using membrane filtration methodology (0.45 μm and 0.22 μm) and then isolated on R2A agar for a period of 5 days and Yeast extract agar growing at 22 °C up to 4 days and 37 °C for 48 hours. The undetected organisms by MALDI-TOF-MS were analysed by PCR and then sequenced. The information obtained by the sequencing was further implemented in the MALDI-TOF-MS library. Among the culturable bacteria, the results show how the incubator temperature affects the growth of some genera instead of others, as demonstrated by Pseudomonas sp., which grows at 22 °C, compared to Bacillus sp., which is abundant at 37 °C. The bacteria community shows a variation in composition also between the media used, as demonstrated with R2A agar which has been defined by a higher presence of organisms not detected compared to YEA. Interesting is the variability of the Genus over one year of sampling and how the seasonality impacts the bacteria community; in fact, in some sampling locations, we observed how the composition changed, moving from winter to spring and summer. In conclusion, the bacteria community in groundwater and river bank filtration represents important information that needs to be added to the library to simplify future water quality analysis but mainly to prevent potential risks to human health. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=water%20quality" title="water quality">water quality</a>, <a href="https://publications.waset.org/abstracts/search?q=MALDI-TOF-MS" title=" MALDI-TOF-MS"> MALDI-TOF-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=sequencing" title=" sequencing"> sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=library" title=" library"> library</a> </p> <a href="https://publications.waset.org/abstracts/166306/one-health-approach-the-importance-of-improving-the-identification-of-waterborne-bacteria-in-austrian-water" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/166306.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">83</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4947</span> Cancer Stem Cell-Associated Serum Proteins Obtained by Maldi TOF/TOF Mass Spectrometry in Women with Triple-Negative Breast Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Javier%20Enciso-Benavides">Javier Enciso-Benavides</a>, <a href="https://publications.waset.org/abstracts/search?q=Fredy%20Fabian"> Fredy Fabian</a>, <a href="https://publications.waset.org/abstracts/search?q=Carlos%20Castaneda"> Carlos Castaneda</a>, <a href="https://publications.waset.org/abstracts/search?q=Luis%20Alfaro"> Luis Alfaro</a>, <a href="https://publications.waset.org/abstracts/search?q=Alex%20Choque"> Alex Choque</a>, <a href="https://publications.waset.org/abstracts/search?q=Aparicio%20Aguilar"> Aparicio Aguilar</a>, <a href="https://publications.waset.org/abstracts/search?q=Javier%20Enciso"> Javier Enciso</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The use of biomarkers in breast cancer diagnosis, therapy, and prognosis has gained increasing interest. Cancer stem cells (CSCs) are a subpopulation of tumor cells that can drive tumor initiation and may cause relapse. Therefore, due to the importance of diagnosis, therapy, and prognosis, several biomarkers that characterize CSCs have been identified; however, in treatment-naïve triple-negative breast tumors, there is an urgent need to identify new biomarkers and therapeutic targets. According to this, the aim of this study was to identify serum proteins associated with cancer stem cells and pluripotency in women with triple-negative breast tumors in order to subsequently identify a biomarker for this type of breast tumor. Material and Methods: Whole blood samples from 12 women with histopathologically diagnosed triple-negative breast tumors were used after obtaining informed consent from the patient. Blood serum was obtained by conventional procedure and frozen at -80ºC. Identification of cancer stem cell-associated proteins was performed by matrix-assisted laser desorption/ionisation-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS), protein analysis was obtained using the AB Sciex TOF/TOF™ 5800 system (AB Sciex, USA). Sequences not aligned by ProteinPilot™ software were analyzed by Protein BLAST. Results: The following proteins related to pluripotency and cancer stem cells were identified by MALDI TOF/TOF mass spectrometry: A-chain, Serpin A12 [Homo sapiens], AIEBP [Homo sapiens], Alpha-one antitrypsin, AT {internal fragment} [human, partial peptide, 20 aa] [Homo sapiens], collagen alpha 1 chain precursor variant [Homo sapiens], retinoblastoma-associated protein variant [Homo sapiens], insulin receptor, CRA_c isoform [Homo sapiens], Hydroxyisourate hydrolase [Streptomyces scopuliridis], MUCIN-6 [Macaca mulatta], Alpha-actinin-3 [Chrysochloris asiatica], Polyprotein M, CRA_d isoform, partial [Homo sapiens], Transcription factor SOX-12 [Homo sapiens]. Recommendations: The serum proteins identified in this study should be investigated in the exosome of triple-negative breast cancer stem cells and in the blood serum of women without breast cancer. Subsequently, proteins found only in the blood serum of women with triple-negative breast cancer should be identified in situ in triple-negative breast cancer tissue in order to identify a biomarker to study the evolution of this type of cancer, or that could be a therapeutic target. Conclusions: Eleven cancer stem cell-related serum proteins were identified in 12 women with triple-negative breast cancer, of which MUCIN-6, retinoblastoma-associated protein variant, transcription factor SOX-12, and collagen alpha 1 chain are the most representative and have not been studied so far in this type of breast tumor. Acknowledgement: This work was supported by Proyecto CONCYTEC–Banco Mundial “Mejoramiento y Ampliacion de los Servicios del Sistema Nacional de Ciencia Tecnología e Innovacion Tecnologica” 8682-PE (104-2018-FONDECYT-BM-IADT-AV). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=triple-negative%20breast%20cancer" title="triple-negative breast cancer">triple-negative breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=MALDI%20TOF%2FTOF%20MS" title=" MALDI TOF/TOF MS"> MALDI TOF/TOF MS</a>, <a href="https://publications.waset.org/abstracts/search?q=serum%20proteins" title=" serum proteins"> serum proteins</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer%20stem%20cells" title=" cancer stem cells"> cancer stem cells</a> </p> <a href="https://publications.waset.org/abstracts/138001/cancer-stem-cell-associated-serum-proteins-obtained-by-maldi-toftof-mass-spectrometry-in-women-with-triple-negative-breast-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/138001.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">215</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4946</span> Comparative Analysis of Benzene, Toluene, Ethylbenzene, and Xylene Concentrations at Roadside and Urban Background Sites in Leicester and Lagos Using Thermal Desorption-Gas Chromatography-Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Emmanuel%20Bernard">Emmanuel Bernard</a>, <a href="https://publications.waset.org/abstracts/search?q=Rebecca%20L.%20Cordell"> Rebecca L. Cordell</a>, <a href="https://publications.waset.org/abstracts/search?q=Akeem%20A.%20Abayomi"> Akeem A. Abayomi</a>, <a href="https://publications.waset.org/abstracts/search?q=Rose%20Alani"> Rose Alani</a>, <a href="https://publications.waset.org/abstracts/search?q=Paul%20S.%20Monks"> Paul S. Monks</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study investigates the prevalence and extent of BTEX (Benzene, Toluene, Ethylbenzene, and Xylene) contamination in Leicester, United Kingdom, and Lagos, Nigeria, through field measurements at roadside (RS) and urban background (UB) sites. Using thermal desorption gas chromatography mass spectrometry (TD-GC-MS), BTEX concentrations were quantified. In Leicester, the average RS concentration was 24.9 ± 8.9 μg/m³, and the UB concentration was 12.7 ± 5.7 μg/m³. In Lagos, the RS concentration was significantly higher at 106 ± 39.3 μg/m³, and the UB concentration was 20.1 ± 8.9 μg/m³. The RS concentration in Lagos was approximately 4.3 times higher than in Leicester, while the UB concentration was about 1.6 times higher. These disparities are attributed to differences in road infrastructure, traffic regulation compliance, fuel and oil quality, and local activities. In Leicester, the highest UB concentration (20.5 ± 1.7 μg/m³) was at Knighton Village, near the heavily polluted RS Wigston roundabout. In Lagos, the highest concentration (172.1 ± 12.2 μg/m³) was at Ojuelegba, a major transportation hub. Correlation analysis revealed strong positive relationships between the concentrations of BTEX compounds in both cities, suggesting common sources such as vehicular emissions and industrial activities. The ratios of toluene to benzene (T:B) and m/p xylene to ethylbenzene (m/p X:E) were analysed to infer source contributions and the photochemical age of air masses. The T:B ratio in Leicester ranged from 0.44 to 0.71, while in Lagos, it ranged from 1.36 to 2.17. The m/p X:E ratio in Leicester ranged from 2.11 to 2.19, like other UK cities, while in Lagos, it ranged from 1.65 to 2.32, indicating relatively fresh emissions. This study highlights significant differences in BTEX concentrations between Leicester and Lagos, emphasizing the need for tailored pollution control strategies to address the specific sources and conditions in different urban environments. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=BTEX%20contamination" title="BTEX contamination">BTEX contamination</a>, <a href="https://publications.waset.org/abstracts/search?q=urban%20air%20quality" title=" urban air quality"> urban air quality</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20desorption%20GC-MS" title=" thermal desorption GC-MS"> thermal desorption GC-MS</a>, <a href="https://publications.waset.org/abstracts/search?q=roadside%20emissions" title=" roadside emissions"> roadside emissions</a>, <a href="https://publications.waset.org/abstracts/search?q=urban%20background%20sites" title=" urban background sites"> urban background sites</a>, <a href="https://publications.waset.org/abstracts/search?q=vehicular%20emissions" title=" vehicular emissions"> vehicular emissions</a>, <a href="https://publications.waset.org/abstracts/search?q=pollution%20control%20strategies" title=" pollution control strategies"> pollution control strategies</a> </p> <a href="https://publications.waset.org/abstracts/186333/comparative-analysis-of-benzene-toluene-ethylbenzene-and-xylene-concentrations-at-roadside-and-urban-background-sites-in-leicester-and-lagos-using-thermal-desorption-gas-chromatography-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186333.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">46</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4945</span> Plasmonic Nanoshells Based Metabolite Detection for in-vitro Metabolic Diagnostics and Therapeutic Evaluation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Deepanjali%20Gurav">Deepanjali Gurav</a>, <a href="https://publications.waset.org/abstracts/search?q=Kun%20Qian"> Kun Qian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In-vitro metabolic diagnosis relies on designed materials-based analytical platforms for detection of selected metabolites in biological samples, which has a key role in disease detection and therapeutic evaluation in clinics. However, the basic challenge deals with developing a simple approach for metabolic analysis in bio-samples with high sample complexity and low molecular abundance. In this work, we report a designer plasmonic nanoshells based platform for direct detection of small metabolites in clinical samples for in-vitro metabolic diagnostics. We first synthesized a series of plasmonic core-shell particles with tunable nanoshell structures. The optimized plasmonic nanoshells as new matrices allowed fast, multiplex, sensitive, and selective LDI MS (Laser desorption/ionization mass spectrometry) detection of small metabolites in 0.5 μL of bio-fluids without enrichment or purification. Furthermore, coupling with isotopic quantification of selected metabolites, we demonstrated the use of these plasmonic nanoshells for disease detection and therapeutic evaluation in clinics. For disease detection, we identified patients with postoperative brain infection through glucose quantitation and daily monitoring by cerebrospinal fluid (CSF) analysis. For therapeutic evaluation, we investigated drug distribution in blood and CSF systems and validated the function and permeability of blood-brain/CSF-barriers, during therapeutic treatment of patients with cerebral edema for pharmacokinetic study. Our work sheds light on the design of materials for high-performance metabolic analysis and precision diagnostics in real cases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plasmonic%20nanoparticles" title="plasmonic nanoparticles">plasmonic nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolites" title=" metabolites"> metabolites</a>, <a href="https://publications.waset.org/abstracts/search?q=fingerprinting" title=" fingerprinting"> fingerprinting</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro%20diagnostics" title=" in-vitro diagnostics"> in-vitro diagnostics</a> </p> <a href="https://publications.waset.org/abstracts/91905/plasmonic-nanoshells-based-metabolite-detection-for-in-vitro-metabolic-diagnostics-and-therapeutic-evaluation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91905.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4944</span> Using MALDI-TOF MS to Detect Environmental Microplastics (Polyethylene, Polyethylene Terephthalate, and Polystyrene) within a Simulated Tissue Sample</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kara%20J.%20Coffman-Rea">Kara J. Coffman-Rea</a>, <a href="https://publications.waset.org/abstracts/search?q=Karen%20E.%20Samonds"> Karen E. Samonds</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Microplastic pollution is an urgent global threat to our planet and human health. Microplastic particles have been detected within our food, water, and atmosphere, and found within the human stool, placenta, and lung tissue. However, most spectrometric microplastic detection methods require chemical digestion which can alter or destroy microplastic particles and makes it impossible to acquire information about their in-situ distribution. MALDI TOF MS (Matrix-assisted laser desorption ionization-time of flight mass spectrometry) is an analytical method using a soft ionization technique that can be used for polymer analysis. This method provides a valuable opportunity to both acquire information regarding the in-situ distribution of microplastics and also minimizes the destructive element of chemical digestion. In addition, MALDI TOF MS allows for expanded analysis of the microplastics including detection of specific additives that may be present within them. MALDI TOF MS is particularly sensitive to sample preparation and has not yet been used to analyze environmental microplastics within their specific location (e.g., biological tissues, sediment, water). In this study, microplastics were created using polyethylene gloves, polystyrene micro-foam, and polyethylene terephthalate cable sleeving. Plastics were frozen using liquid nitrogen and ground to obtain small fragments. An artificial tissue was created using a cellulose sponge as scaffolding coated with a MaxGel Extracellular Matrix to simulate human lung tissue. Optimal preparation techniques (e.g., matrix, cationization reagent, solvent, mixing ratio, laser intensity) were first established for each specific polymer type. The artificial tissue sample was subsequently spiked with microplastics, and specific polymers were detected using MALDI-TOF-MS. This study presents a novel method for the detection of environmental polyethylene, polyethylene terephthalate, and polystyrene microplastics within a complex sample. Results of this study provide an effective method that can be used in future microplastics research and can aid in determining the potential threats to environmental and human health that they pose. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=environmental%20plastic%20pollution" title="environmental plastic pollution">environmental plastic pollution</a>, <a href="https://publications.waset.org/abstracts/search?q=MALDI-TOF%20MS" title=" MALDI-TOF MS"> MALDI-TOF MS</a>, <a href="https://publications.waset.org/abstracts/search?q=microplastics" title=" microplastics"> microplastics</a>, <a href="https://publications.waset.org/abstracts/search?q=polymer%20identification" title=" polymer identification"> polymer identification</a> </p> <a href="https://publications.waset.org/abstracts/141577/using-maldi-tof-ms-to-detect-environmental-microplastics-polyethylene-polyethylene-terephthalate-and-polystyrene-within-a-simulated-tissue-sample" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141577.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">256</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4943</span> Determination of Brominated Flame Retardants In Recycled Plastic Toys Using Thermal Desorption GC/MS</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Athena%20Nguyen">Athena Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Rojin%20Belganeh"> Rojin Belganeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In recycling plastics industries, waste plastics are converted into monomers and other useful molecules by chemical reactions. Thermal energy generated by incineration is recovered when waste plastics melt. During the process, Flame retardants containing products get in, and brominated flame retardants (BFRs) are often used to reduce the flammability of products. Some of the originally formulated brominated flame retardants additives are restricted by the RoHS Directive, such as PBDE and PBB. The determination of BFRs other than those restricted by the RoHS directive is required. Frontier Lab developed a pyrolyzer based on the vertical micro-furnace design. The multi-mode pyrolyzer with different modes of operations, including evolve gas analysis (EGA), flash pyrolysis, thermal desorption, heart cutting, allows users to choose among the techniques for their analysis purposes. The method requires very little sample preparation. The first step is to perform an EGA using temperature programs. This technique provides information about the thermal temperature behaviors of the sample. The EGA thermogram is then used to determine the next steps in the analysis process. In this presentation, with an Optimal thermal temperature zone identified based on EGA thermogram, thermal desorption GC/MS is a chosen technique for the determination of brominated flame retardants in recycled plastic toys. Five types of general-purpose brominated flame retardants other than those restricted by the RoHS Directive are determined by the standard addition method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography%2Fmass%20spectrometry" title="gas chromatography/mass spectrometry">gas chromatography/mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolysis" title=" pyrolysis"> pyrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolyzer" title=" pyrolyzer"> pyrolyzer</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20desorption-GC%2FMS" title=" thermal desorption-GC/MS"> thermal desorption-GC/MS</a> </p> <a href="https://publications.waset.org/abstracts/139715/determination-of-brominated-flame-retardants-in-recycled-plastic-toys-using-thermal-desorption-gcms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139715.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">193</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4942</span> Purification and Characterization of a Novel Extracellular Chitinase from Bacillus licheniformis LHH100</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Laribi-Habchi%20Hasiba">Laribi-Habchi Hasiba</a>, <a href="https://publications.waset.org/abstracts/search?q=Bouanane-Darenfed%20Amel"> Bouanane-Darenfed Amel</a>, <a href="https://publications.waset.org/abstracts/search?q=Drouiche%20Nadjib"> Drouiche Nadjib</a>, <a href="https://publications.waset.org/abstracts/search?q=Pausse%20Andr%C3%A9"> Pausse André</a>, <a href="https://publications.waset.org/abstracts/search?q=Mameri%20Nabil"> Mameri Nabil</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Chitin, a linear 1, 4-linked N-acetyl-d-glucosamine (GlcNAc) polysaccharide is the major structural component of fungal cell walls, insect exoskeletons and shells of crustaceans. It is one of the most abundant naturally occurring polysaccharides and has attracted tremendous attention in the fields of agriculture, pharmacology and biotechnology. Each year, a vast amount of chitin waste is released from the aquatic food industry, where crustaceans (prawn, crab, Shrimp and lobster) constitute one of the main agricultural products. This creates a serious environmental problem. This linear polymer can be hydrolyzed by bases, acids or enzymes such as chitinase. In this context an extracellular chitinase (ChiA-65) was produced and purified from a newly isolated LHH100. Pure protein was obtained after heat treatment and ammonium sulphate precipitation followed by Sephacryl S-200 chromatography. Based on matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis, the purified enzyme is a monomer with a molecular mass of 65,195.13 Da. The sequence of the 27 N-terminal residues of the mature ChiA-65 showed high homology with family-18 chitinases. Optimal activity was achieved at pH 4 and 75◦C. Among the inhibitors and metals tested p-chloromercuribenzoic acid, N-ethylmaleimide, Hg2+ and Hg + completelyinhibited enzyme activity. Chitinase activity was high on colloidal chitin, glycol chitin, glycol chitosane, chitotriose and chitooligosaccharide. Chitinase activity towards synthetic substrates in the order of p-NP-(GlcNAc) n (n = 2–4) was p-NP-(GlcNAc)2> p-NP-(GlcNAc)4> p-NP-(GlcNAc)3. Our results suggest that ChiA-65 preferentially hydrolyzed the second glycosidic link from the non-reducing end of (GlcNAc) n. ChiA-65 obeyed Michaelis Menten kinetics the Km and kcat values being 0.385 mg, colloidal chitin/ml and5000 s−1, respectively. ChiA-65 exhibited remarkable biochemical properties suggesting that this enzyme is suitable for bioconversion of chitin waste. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bacillus%20licheniformis%20LHH100" title="Bacillus licheniformis LHH100">Bacillus licheniformis LHH100</a>, <a href="https://publications.waset.org/abstracts/search?q=characterization" title=" characterization"> characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=extracellular%20chitinase" title=" extracellular chitinase"> extracellular chitinase</a>, <a href="https://publications.waset.org/abstracts/search?q=purification" title=" purification "> purification </a> </p> <a href="https://publications.waset.org/abstracts/27008/purification-and-characterization-of-a-novel-extracellular-chitinase-from-bacillus-licheniformis-lhh100" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27008.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">437</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4941</span> The Tadpole-Shaped Polypeptides with Two Regulable (Alkyl Chain) Tails</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hua%20Jin">Hua Jin</a>, <a href="https://publications.waset.org/abstracts/search?q=Il%20Kim"> Il Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The biocompatible tadpole-shaped polypeptides with one cyclic polypeptides ring and two alkyl chain tails were synthesized by N-heterocyclic carbine (NHC)-mediated ring-opening polymerization (ROP) of α-amino acid N-carboxyanhydrides (NCAs). First, the NHC precursor, denoted as [NHC(H)][HCO₃], with two alkyl chains at the nitrogen was prepared by a simple anion metathesis of imidazole(in)ium chlorides with KHCO₃. Then NHC releasing from the [NHC(H)][HCO₃] directly initiated the ROP of NCA to produce the cyclic polypeptides. Finally, the tadpole-shaped polypeptides with two regulable tails were obtained. The target polypeptides were characterized by nuclear magnetic resonance spectrum (1H NMR), Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatography (GPC) and matrix-assisted laser desorption ionization-time of flight mass spectra (MALDI-TOF MS). This pioneering approach simplifies the synthesis procedures of tadpole-shaped polypeptides compared to other methods, which usually requires specific intramolecular ring-closure reaction. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cyclic%20polypeptides" title="cyclic polypeptides">cyclic polypeptides</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-amino%20acid%20N-carboxyanhydrides" title=" α-amino acid N-carboxyanhydrides"> α-amino acid N-carboxyanhydrides</a>, <a href="https://publications.waset.org/abstracts/search?q=N-heterocyclic%20carbene" title=" N-heterocyclic carbene"> N-heterocyclic carbene</a>, <a href="https://publications.waset.org/abstracts/search?q=ring-opening%20polymerization" title=" ring-opening polymerization"> ring-opening polymerization</a>, <a href="https://publications.waset.org/abstracts/search?q=tadpole-shaped" title=" tadpole-shaped"> tadpole-shaped</a> </p> <a href="https://publications.waset.org/abstracts/75743/the-tadpole-shaped-polypeptides-with-two-regulable-alkyl-chain-tails" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75743.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">205</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4940</span> Bulk Amounts of Linear and Cyclic Polypeptides on Our Hand within a Short Time</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yu%20Zhang">Yu Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Il%20Kim"> Il Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Polypeptides with defined peptide sequences illustrate the power of remarkable applications in drug delivery, tissue engineering, sensing and catalysis. Especially the cyclic polypeptides, the distinctive topological architecture imparts many characteristic properties comparing to linear polypeptides. Here, a facile and highly efficient strategy for the synthesis of linear and cyclic polypeptides is reported using N-heterocyclic carbenes (NHCs)-mediated ring-opening polymerization (ROP) of α-amino acid N-carboxyanhydrides (NCA) in the presence or absence of primary amine initiator. The polymerization proceeds rapidly in a quasi-living manner, allowing access to linear and cyclic polypeptides of well-defined chain length and narrow polydispersity, as evidenced by nuclear magnetic resonance spectrum (1H NMR and 13C NMR spectra) and size exclusion chromatography (SEC) analysis. The cyclic architecture of the polypeptides was further verified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra (MALDI-TOF MS) and electrospray ionization (ESI) mass spectra, as well as viscosity studies. This approach can also simplify workup procedures and make bulk scale synthesis possible, which thereby opens avenues for practical uses in diverse areas, opening up the new generation of polypeptide synthesis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-amino%20acid%20N-carboxyanhydrides" title="α-amino acid N-carboxyanhydrides">α-amino acid N-carboxyanhydrides</a>, <a href="https://publications.waset.org/abstracts/search?q=living%20polymerization" title=" living polymerization"> living polymerization</a>, <a href="https://publications.waset.org/abstracts/search?q=polypeptides" title=" polypeptides"> polypeptides</a>, <a href="https://publications.waset.org/abstracts/search?q=N-heterocyclic%20carbenes" title=" N-heterocyclic carbenes"> N-heterocyclic carbenes</a>, <a href="https://publications.waset.org/abstracts/search?q=ring-opening%20polymerization" title=" ring-opening polymerization"> ring-opening polymerization</a> </p> <a href="https://publications.waset.org/abstracts/75665/bulk-amounts-of-linear-and-cyclic-polypeptides-on-our-hand-within-a-short-time" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75665.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">167</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4939</span> Basic One-Dimensional Modelica®-Model for Simulation of Gas-Phase Adsorber Dynamics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adrian%20Rettig">Adrian Rettig</a>, <a href="https://publications.waset.org/abstracts/search?q=Silvan%20Schneider"> Silvan Schneider</a>, <a href="https://publications.waset.org/abstracts/search?q=Reto%20Tamburini"> Reto Tamburini</a>, <a href="https://publications.waset.org/abstracts/search?q=Mirko%20Kleingries"> Mirko Kleingries</a>, <a href="https://publications.waset.org/abstracts/search?q=Ulf%20Christian%20Muller">Ulf Christian Muller</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Industrial adsorption processes are, mainly due to si-multaneous heat and mass transfer, characterized by a high level of complexity. The conception of such processes often does not take place systematically; instead scale-up/down respectively number-up/down methods based on existing systems are used. This paper shows how Modelica® can be used to develop a transient model enabling a more systematic design of such ad- and desorption components and processes. The core of this model is a lumped-element submodel of a single adsorbent grain, where the thermodynamic equilibria and the kinetics of the ad- and desorption processes are implemented and solved on the basis of mass-, momentum and energy balances. For validation of this submodel, a fixed bed adsorber, whose characteristics are described in detail in the literature, was modeled and simulated. The simulation results are in good agreement with the experimental results from the literature. Therefore, the model development will be continued, and the extended model will be applied to further adsorber types like rotor adsorbers and moving bed adsorbers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adsorption" title="adsorption">adsorption</a>, <a href="https://publications.waset.org/abstracts/search?q=desorption" title=" desorption"> desorption</a>, <a href="https://publications.waset.org/abstracts/search?q=linear%20driving%20force" title=" linear driving force"> linear driving force</a>, <a href="https://publications.waset.org/abstracts/search?q=dynamic%20model" title=" dynamic model"> dynamic model</a>, <a href="https://publications.waset.org/abstracts/search?q=Modelica%C2%AE" title=" Modelica®"> Modelica®</a>, <a href="https://publications.waset.org/abstracts/search?q=integral%20equation%20approach" title=" integral equation approach"> integral equation approach</a> </p> <a href="https://publications.waset.org/abstracts/130750/basic-one-dimensional-modelica-model-for-simulation-of-gas-phase-adsorber-dynamics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/130750.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">371</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4938</span> New Method for the Determination of Montelukast in Human Plasma by Solid Phase Extraction Using Liquid Chromatography Tandem Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vijayalakshmi%20Marella">Vijayalakshmi Marella</a>, <a href="https://publications.waset.org/abstracts/search?q=NageswaraRaoPilli"> NageswaraRaoPilli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper describes a simple, rapid and sensitive liquid chromatography / tandem mass spectrometry assay for the determination of montelukast in human plasma using montelukast d6 as an internal standard. Analyte and the internal standard were extracted from 50 µL of human plasma via solid phase extraction technique without evaporation, drying and reconstitution steps. The chromatographic separation was achieved on a C18 column by using a mixture of methanol and 5mM ammonium acetate (80:20, v/v) as the mobile phase at a flow rate of 0.8 mL/min. Good linearity results were obtained during the entire course of validation. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more number of samples in short time, thus increasing the productivity. The proposed method was found to be applicable to clinical studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Montelukast" title="Montelukast">Montelukast</a>, <a href="https://publications.waset.org/abstracts/search?q=tandem%20mass%20spectrometry" title=" tandem mass spectrometry"> tandem mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=montelukast%20d6" title=" montelukast d6"> montelukast d6</a>, <a href="https://publications.waset.org/abstracts/search?q=FDA%20guidelines" title=" FDA guidelines"> FDA guidelines</a> </p> <a href="https://publications.waset.org/abstracts/29304/new-method-for-the-determination-of-montelukast-in-human-plasma-by-solid-phase-extraction-using-liquid-chromatography-tandem-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29304.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">315</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4937</span> Laser Registration and Supervisory Control of neuroArm Robotic Surgical System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hamidreza%20Hoshyarmanesh">Hamidreza Hoshyarmanesh</a>, <a href="https://publications.waset.org/abstracts/search?q=Hosein%20Madieh"> Hosein Madieh</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanju%20Lama"> Sanju Lama</a>, <a href="https://publications.waset.org/abstracts/search?q=Yaser%20Maddahi"> Yaser Maddahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Garnette%20R.%20Sutherland"> Garnette R. Sutherland</a>, <a href="https://publications.waset.org/abstracts/search?q=Kourosh%20Zareinia"> Kourosh Zareinia</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper illustrates the concept of an algorithm to register specified markers on the neuroArm surgical manipulators, an image-guided MR-compatible tele-operated robot for microsurgery and stereotaxy. Two range-finding algorithms, namely time-of-flight and phase-shift, are evaluated for registration and supervisory control. The time-of-flight approach is implemented in a semi-field experiment to determine the precise position of a tiny retro-reflective moving object. The moving object simulates a surgical tool tip. The tool is a target that would be connected to the neuroArm end-effector during surgery inside the magnet bore of the MR imaging system. In order to apply flight approach, a 905-nm pulsed laser diode and an avalanche photodiode are utilized as the transmitter and receiver, respectively. For the experiment, a high frequency time to digital converter was designed using a field-programmable gate arrays. In the phase-shift approach, a continuous green laser beam with a wavelength of 530 nm was used as the transmitter. Results showed that a positioning error of 0.1 mm occurred when the scanner-target point distance was set in the range of 2.5 to 3 meters. The effectiveness of this non-contact approach exhibited that the method could be employed as an alternative for conventional mechanical registration arm. Furthermore, the approach is not limited by physical contact and extension of joint angles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=3D%20laser%20scanner" title="3D laser scanner">3D laser scanner</a>, <a href="https://publications.waset.org/abstracts/search?q=intraoperative%20MR%20imaging" title=" intraoperative MR imaging"> intraoperative MR imaging</a>, <a href="https://publications.waset.org/abstracts/search?q=neuroArm" title=" neuroArm"> neuroArm</a>, <a href="https://publications.waset.org/abstracts/search?q=real%20time%20registration" title=" real time registration"> real time registration</a>, <a href="https://publications.waset.org/abstracts/search?q=robot-assisted%20surgery" title=" robot-assisted surgery"> robot-assisted surgery</a>, <a href="https://publications.waset.org/abstracts/search?q=supervisory%20control" title=" supervisory control"> supervisory control</a> </p> <a href="https://publications.waset.org/abstracts/70477/laser-registration-and-supervisory-control-of-neuroarm-robotic-surgical-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/70477.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">286</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4936</span> Sampling and Chemical Characterization of Particulate Matter in a Platinum Mine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Juergen%20Orasche">Juergen Orasche</a>, <a href="https://publications.waset.org/abstracts/search?q=Vesta%20Kohlmeier"> Vesta Kohlmeier</a>, <a href="https://publications.waset.org/abstracts/search?q=George%20C.%20Dragan"> George C. Dragan</a>, <a href="https://publications.waset.org/abstracts/search?q=Gert%20Jakobi"> Gert Jakobi</a>, <a href="https://publications.waset.org/abstracts/search?q=Patricia%20Forbes"> Patricia Forbes</a>, <a href="https://publications.waset.org/abstracts/search?q=Ralf%20Zimmermann"> Ralf Zimmermann</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Underground mining poses a difficult environment for both man and machines. At more than 1000 meters underneath the surface of the earth, ores and other mineral resources are still gained by conventional and motorised mining. Adding to the hazards caused by blasting and stone-chipping, the working conditions are best described by the high temperatures of 35-40°C and high humidity, at low air exchange rates. Separate ventilation shafts lead fresh air into a mine and others lead expended air back to the surface. This is essential for humans and machines working deep underground. Nevertheless, mines are widely ramified. Thus the air flow rate at the far end of a tunnel is sensed to be close to zero. In recent years, conventional mining was supplemented by mining with heavy diesel machines. These very flat machines called Load Haul Dump (LHD) vehicles accelerate and ease work in areas favourable for heavy machines. On the other hand, they emit non-filtered diesel exhaust, which constitutes an occupational hazard for the miners. Combined with a low air exchange, high humidity and inorganic dust from the mining it leads to 'black smog' underneath the earth. This work focuses on the air quality in mines employing LHDs. Therefore we performed personal sampling (samplers worn by miners during their work), stationary sampling and aethalometer (Microaeth MA200, Aethlabs) measurements in a platinum mine in around 1000 meters under the earth’s surface. We compared areas of high diesel exhaust emission with areas of conventional mining where no diesel machines were operated. For a better assessment of health risks caused by air pollution we applied a separated gas-/particle-sampling tool (or system), with first denuder section collecting intermediate VOCs. These multi-channel silicone rubber denuders are able to trap IVOCs while allowing particles ranged from 10 nm to 1 µm in diameter to be transmitted with an efficiency of nearly 100%. The second section is represented by a quartz fibre filter collecting particles and adsorbed semi-volatile organic compounds (SVOC). The third part is a graphitized carbon black adsorber – collecting the SVOCs that evaporate from the filter. The compounds collected on these three sections were analyzed in our labs with different thermal desorption techniques coupled with gas chromatography and mass spectrometry (GC-MS). VOCs and IVOCs were measured with a Shimadzu Thermal Desorption Unit (TD20, Shimadzu, Japan) coupled to a GCMS-System QP 2010 Ultra with a quadrupole mass spectrometer (Shimadzu). The GC was equipped with a 30m, BP-20 wax column (0.25mm ID, 0.25µm film) from SGE (Australia). Filters were analyzed with In-situ derivatization thermal desorption gas chromatography time-of-flight-mass spectrometry (IDTD-GC-TOF-MS). The IDTD unit is a modified GL sciences Optic 3 system (GL Sciences, Netherlands). The results showed black carbon concentrations measured with the portable aethalometers up to several mg per m³. The organic chemistry was dominated by very high concentrations of alkanes. Typical diesel engine exhaust markers like alkylated polycyclic aromatic hydrocarbons were detected as well as typical lubrication oil markers like hopanes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diesel%20emission" title="diesel emission">diesel emission</a>, <a href="https://publications.waset.org/abstracts/search?q=personal%20sampling" title=" personal sampling"> personal sampling</a>, <a href="https://publications.waset.org/abstracts/search?q=aethalometer" title=" aethalometer"> aethalometer</a>, <a href="https://publications.waset.org/abstracts/search?q=mining" title=" mining"> mining</a> </p> <a href="https://publications.waset.org/abstracts/86968/sampling-and-chemical-characterization-of-particulate-matter-in-a-platinum-mine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86968.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info 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