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for="set">Set</label></th> <td class="value"> <select id="set" class="" name="attribute_set" data-attribute_name="attribute_set" data-show_option_none="yes"><option value="">Choose Set</option><option value="Any Barcode Set" >Any Barcode Set</option><option value="Set 1 - SKU 301067" >Set 1 - SKU 301067</option><option value="Set 2 - SKU 301068" >Set 2 - SKU 301068</option><option value="Set 3 - SKU 301069" >Set 3 - SKU 301069</option><option value="Set 4 - SKU 301070" >Set 4 - SKU 301070</option></select><a class="reset_variations" href="#">Clear</a> </td> </tr> </tbody> </table> <div class="single_variation_wrap"> <div class="woocommerce-variation single_variation"></div><div class="woocommerce-variation-add-to-cart variations_button"> <div class="quantity"> <label class="screen-reader-text" for="quantity_674ddb35ddcf4">purePlex™ DNA Library Prep Kit quantity</label> <input type="number" id="quantity_674ddb35ddcf4" class="input-text qty text" name="quantity" value="1" aria-label="Product quantity" size="4" min="1" max="" step="1" placeholder="" inputmode="numeric" autocomplete="off" /> </div> <button type="submit" class="single_add_to_cart_button button alt">Login or Register</button> <input type="hidden" name="gtm4wp_product_data" value="{"internal_id":4108,"item_id":4108,"item_name":"purePlex\u2122 DNA Library Prep Kit","sku":"301067","price":2470,"stocklevel":null,"stockstatus":"instock","google_business_vertical":"retail","item_category":"Uncategorized","id":4108}" /> <input type="hidden" name="add-to-cart" value="4108" /> <input type="hidden" name="product_id" value="4108" /> <input type="hidden" name="variation_id" class="variation_id" value="0" /> </div> </div> </form> <div class="Request-Quote"> <a class="single_add_to_cart_button button alt" href="https://seqwell.com/request-quote/">Request Quote</a> </div> <div class="content-wrap"> <div class="woocommerce-product-details__short-description"> <h4 class="p1"><i>Speed, Performance, and Auto-Normalization with Unique Dual Indexes</i></h4> <ul class="ul1"> <li class="li1">2.5-hour workflow for 96 samples, 45 min. hands-on time</li> <li class="li1">Auto-normalization of read counts and insert size over 10-fold input range</li> <li class="li1">Unique dual indices included</li> </ul> </div> <a class="underline-link" target="_BLANK" href="https://seqwell.com/wp-content/uploads/2024/03/seqWell-Kit-Comparison-Guide.png">View Product Comparison Chart</a> </div> </div> </div> </header><section class="content-section gray" id="content1"> <div class="contain wide"> <div class="content-wrap"> <h2 class="p1" style="text-align: center;">A benefit to purePlex is that, because of its simplicity, users can quickly and seamlessly incorporate the kit with existing methods for high-throughput pipetting.</h2> </div> </div> </section> <section class="content-section white" id="content2"> <div class="contain wide"> <div class="col-1-2 content-wrap"> <h3>Key Features</h3> <ul> <li>Fast, flexible workflow with no requirement for full plate processing</li> <li>Auto-normalization reduces QC burden, improves data consistency</li> <li>Early pooling for easier sample handling</li> <li>Reduced GC bias compared to other transposase-based methods</li> <li>Significant cost and plastics savings</li> </ul> </div> <div class="col-1-2 content-wrap right"> <h4 style="text-align: center;"> Streamlined Workflow</h4> <p><img decoding="async" class="aligncenter size-large wp-image-4399" src="https://seqwell.com/wp-content/uploads/2022/04/pureplex-workflow-diagram-1200x1000-1-1024x776.png" alt="" width="1024" height="776" srcset="https://seqwell.com/wp-content/uploads/2022/04/pureplex-workflow-diagram-1200x1000-1-1024x776.png 1024w, https://seqwell.com/wp-content/uploads/2022/04/pureplex-workflow-diagram-1200x1000-1-300x227.png 300w, https://seqwell.com/wp-content/uploads/2022/04/pureplex-workflow-diagram-1200x1000-1-768x582.png 768w, https://seqwell.com/wp-content/uploads/2022/04/pureplex-workflow-diagram-1200x1000-1-1536x1164.png 1536w, https://seqwell.com/wp-content/uploads/2022/04/pureplex-workflow-diagram-1200x1000-1-600x455.png 600w, https://seqwell.com/wp-content/uploads/2022/04/pureplex-workflow-diagram-1200x1000-1.png 1666w" sizes="(max-width: 1024px) 100vw, 1024px" /></p> </div> </div> </section> <section class="content-section gray" id="content3"> <div class="contain "> <div class="content-wrap"> <h3>Check out what our customers are saying:</h3> </div> </div> <div class="contain "> <div class="image-wrap fade right in-left col-1-2"> <img src="https://seqwell.com/wp-content/uploads/2022/04/AUG-23-Sophie-NWU-Quote-V06-TECH-PAGE-1200px-800px-1024x683.jpg" alt="" /> </div> <div class="content-wrap col-1-2"> <p>“I’ve always used i5 and i7 barcodes. Other library prep methods incorporated a pool barcode so only one was unique. With purePlex the i5 and i7 are unique to every single sample so I can catch barcode slippage much more easily. It makes me feel a lot more comfortable. purePlex is also a lot quicker than the Nextera kits we’ve used in the past. Now we use a lot less tips and can go from DNA to having our library in a small tube by the end of the day then having it sequenced the next day. It’s amazing.”</p> <p><strong>– Sophie Nozick</strong></p> </div> </div> <div class="contain "> <div class="image-wrap fade in-right col-1-2"> <img src="https://seqwell.com/wp-content/uploads/2022/04/UMN-Ray-Watson-Quote-RV01-WEBSITE-IMAGE-1200px-800px-1024x683.jpg" alt="" /> </div> <div class="content-wrap right col-1-2"> <p>“The sample auto-normalization with purePlex simplifies our sample balancing process without seeing evidence of substantial bottlenecking of unique reads or an overabundance of PCR duplicates. That’s very good for us because it means we are not wasting sequencing data on duplicate information or on imbalanced samples. We found that, in general, purePlex was either comparable or performed better than other methods for both balance and low duplication.”</p> <p><strong>– Ray Watson</strong></p> </div> </div> </section> <section class="content-section blue" id="content4"> <div class="contain narrow"> <div class="cta-block fade in-up"> <div class="flex"> <header class="content-wrap"> <p><span style="color: #ffffff;">Learn how the purePlex DNA library prep system offers speed, performance, and auto-normalization with unique dual indexes with Maura Costello, Team Leader, R&D, at seqWell.</span></p> </header> <footer> <a class="button" href="https://seqwell.wistia.com/medias/sgojq1ryk7" target="_blank">WATCH VIDEO</a> </footer> </div> </div> </div> </section> <section class="content-section white" id="content5"> <div class="contain wide"> <div class="content-wrap"> <h3>Product Highlights</h3> <p class="p1">Auto-Normalization of Insert Size and Read Depth:</p> </div> </div> <div class="contain wide"> <div class="col-1-2 content-wrap"> <div style="background-color: white; padding: 40px 30px; box-shadow: 0 0 2em -0.5em rgb(0, 0, 0, 20%); height: 480px; border-radius: .5em;"> <p><img decoding="async" class="alignnone size-full wp-image-4166" src="https://seqwell.com/wp-content/uploads/2022/03/Insert-A-700x500-2.png" alt="" width="700" height="500" srcset="https://seqwell.com/wp-content/uploads/2022/03/Insert-A-700x500-2.png 700w, https://seqwell.com/wp-content/uploads/2022/03/Insert-A-700x500-2-300x214.png 300w, https://seqwell.com/wp-content/uploads/2022/03/Insert-A-700x500-2-600x429.png 600w" sizes="(max-width: 700px) 100vw, 700px" /></p> </div> </div> <div class="col-1-2 content-wrap right"> <div style="background-color: white; padding: 40px 30px; box-shadow: 0 0 2em -0.5em rgb(0, 0, 0, 20%); height: 480px; border-radius: .5em;"> <p class="p1">The insert size within a pool of samples is consistent regardless of input (panel A, left) or GC content (panel B, lower left).<span class="Apple-converted-space"> </span>In contrast, Nextera XT libraries have varied fragment distributions from GC content even after normalizing the sample input.<span class="Apple-converted-space"> </span></p> </div> </div> </div> <div class="contain wide"> <div class="col-1-2 content-wrap"> <div style="background-color: white; padding: 40px 30px; box-shadow: 0 0 2em -0.5em rgb(0, 0, 0, 20%); height: 480px; border-radius: .5em;"> <p><img loading="lazy" decoding="async" class="alignnone wp-image-4117 size-full" src="https://seqwell.com/wp-content/uploads/2022/03/Insert-B-700x500-1.png" alt="" width="700" height="500" srcset="https://seqwell.com/wp-content/uploads/2022/03/Insert-B-700x500-1.png 700w, https://seqwell.com/wp-content/uploads/2022/03/Insert-B-700x500-1-300x214.png 300w, https://seqwell.com/wp-content/uploads/2022/03/Insert-B-700x500-1-600x429.png 600w" sizes="(max-width: 700px) 100vw, 700px" /></p> <p> </p> </div> </div> <div class="col-1-2 content-wrap right"> <div style="background-color: white; padding: 40px 30px; box-shadow: 0 0 2em -0.5em rgb(0, 0, 0, 20%); height: 480px; border-radius: .5em;"> <p><img loading="lazy" decoding="async" class="alignnone wp-image-4116 size-full" src="https://seqwell.com/wp-content/uploads/2022/03/Insert-C-700x500-1.png" alt="" width="700" height="500" srcset="https://seqwell.com/wp-content/uploads/2022/03/Insert-C-700x500-1.png 700w, https://seqwell.com/wp-content/uploads/2022/03/Insert-C-700x500-1-300x214.png 300w, https://seqwell.com/wp-content/uploads/2022/03/Insert-C-700x500-1-600x429.png 600w" sizes="(max-width: 700px) 100vw, 700px" /></p> </div> </div> </div> </section> <section class="content-section gray" id="content6"> <div class="contain wide"> <div class="content-wrap"> <h3>Auto-normalization of Read Depth</h3> </div> </div> <div class="contain wide"> <div class="col-3-5 content-wrap"> <p><img loading="lazy" decoding="async" class="alignnone wp-image-4119 size-full" src="https://seqwell.com/wp-content/uploads/2022/03/dna-input-titration-700x500-1.png" alt="" width="700" height="500" srcset="https://seqwell.com/wp-content/uploads/2022/03/dna-input-titration-700x500-1.png 700w, https://seqwell.com/wp-content/uploads/2022/03/dna-input-titration-700x500-1-300x214.png 300w, https://seqwell.com/wp-content/uploads/2022/03/dna-input-titration-700x500-1-600x429.png 600w" sizes="(max-width: 700px) 100vw, 700px" /></p> </div> <div class="col-2-5 content-wrap right"> <p class="p1">Samples were normalized to inputs of 3, 5, 10, and 30 ng then underwent purePlex library prep with (<b>+</b>) and without (<b>–</b>) normalization reagent. Read counts for each sample are equal, regardless of input, when normalization reagent is used. In contrast, without normalization reagent, sample read count scales with input.</p> </div> </div> </section> <section class="content-section white" id="content7"> <div class="contain wide"> <div class="content-wrap"> <p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-4115" src="https://seqwell.com/wp-content/uploads/2022/03/reduced-GC-Bias-1200x750-1.png" alt="" width="1200" height="750" srcset="https://seqwell.com/wp-content/uploads/2022/03/reduced-GC-Bias-1200x750-1.png 1200w, https://seqwell.com/wp-content/uploads/2022/03/reduced-GC-Bias-1200x750-1-300x188.png 300w, https://seqwell.com/wp-content/uploads/2022/03/reduced-GC-Bias-1200x750-1-1024x640.png 1024w, https://seqwell.com/wp-content/uploads/2022/03/reduced-GC-Bias-1200x750-1-768x480.png 768w, https://seqwell.com/wp-content/uploads/2022/03/reduced-GC-Bias-1200x750-1-600x375.png 600w" sizes="(max-width: 1200px) 100vw, 1200px" /></p> </div> </div> </section> <section class="content-section product-content"> <div class="contain wide"> <div class="col-1-2 content-wrap"> <h2>Specifications</h2> <table style="width: 85%;"> <tbody> <tr> <th>Specs</th> <th>Description</th> </tr> <tr> <td>Sample Type</td> <td>Amplicons, Plasmids, Genomic DNA, cDNA</td> </tr> <tr> <td>DNA Input Range</td> <td>5 – 50 ng</td> </tr> <tr> <td>Number of Unique Index Combinations</td> <td>384</td> </tr> <tr> <td>Supported Paired Reads (Clusters)/Sample</td> <td>≤ 20 million</td> </tr> <tr> <td>Output Fragment Range</td> <td>400 – 1,200 bp</td> </tr> <tr> <td>Applications</td> <td>Synthetic construct sequencing (amplicons, plasmids, etc.), Low-pass whole genome sequencing, Whole small genome sequencing (<50 Mb), scRNA-seq, Metagenomics/Microbiome screening</td> </tr> <tr> <td>Reactions per Kit</td> <td>96</td> </tr> <tr> <td></td> <td></td> </tr> <tr> <td></td> <td></td> </tr> </tbody> </table> <p> </p> <h4 class="p1">purePlex DNA Library Prep Kit Includes:</h4> <ul class="ul1"> <li class="li1">i7 Tagging Reagent Plate</li> <li class="li1">i5 Tagging Reagent Plate</li> <li class="li1">Coding Buffer (3X)</li> <li class="li1">X Solution</li> <li class="li1">MAGwise Paramagnetic Beads</li> <li class="li1">Normalization Reagent</li> <li class="li1">Library Primer Mix</li> </ul> </div> <div class="col-1-2"> <header class="section-header"> <h2>purePlex™ DNA Library Prep Kit Resources</h2> </header> <a class="resource-wrap" href="https://5527966.app.netsuite.com/core/media/media.nl?id=240971&c=5527966&h=8DEBLYbY--LcuJ7-bFx0uc54iWzP0CchHHoQySLgqeYJxLGy&_xt=.pdf" target="_blank" > <h4>purePlex DNA Library Prep Kit User Guide</h4> </a> <a class="resource-wrap" href="https://5527966.app.netsuite.com/core/media/media.nl?id=240972&c=5527966&h=kP8C5tN84-V8dRcjdyXxWmPDgDGLKCc7GfgSBE-3_Jf6kwNa&_xt=.xls" target="_blank" > <h4>purePlex Kit Master Index List</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2022/11/purePlex-Brochure-V10-8.5-11-D-Single-brand-REV-7-31-23.pdf" target="_blank" > <h4>purePlex Product Brochure</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2022/06/2022_poster_UDIs_purePlex.pdf" target="_blank" > <h4>Normalizing UDI library construction for sensitive genomic applications</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2023/03/SW2023_TAMU-Cannabis-AGBT-AG-FIN2-8.5x11.pdf" target="_blank" > <h4>An Automated Workflow for Multiplexed NGS Library Construction in Low-Coverage Whole-Genome Sequencing of Cannabis sativa</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2023/02/SW2023_purePlex-AVITI-AN-02062023v2.pdf" target="_blank" > <h4>Low-Pass Whole-Genome Sequencing on the Element AVITI™ System Enables Cost-Effective Genotyping</h4> </a> <a class="resource-wrap" href="https://seqwell.com/wp-content/uploads/2024/01/SW2024_ASHG-Genomics-CollaborationI-AN-10272023.pdf" target="_blank" > <h4>Scalable Transposase-Based Multiplexed Library Prep with Auto-Normalizing UDI Capability Using purePlex™ on the Complete Genomics™ DNBSEQ-G400 Genetic Sequencer</h4> </a> </div> </div> </section> <footer id="bottom"> <div class="contain wide flex"> <div class="col-1-4 content-wrap"> <h4>Office</h4> <p>66 Cherry Hill Drive<br /> Beverly, MA 01915<br /> 1 (855) SEQWELL (737-9355)</p> <p><script src="https://kit.fontawesome.com/0e0cfcdfba.js" crossorigin="anonymous"></script></p> <style> .footer-socials a { display: inline-block; 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