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Manufacturing remains arduous, especially for DC vaccines, and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16371687" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Cell-based active immunotherapy for cancer is a promising novel strategy, with the first dendritic cell (DC) vaccine achieving regulatory approval for clinical use last year. Manufacturing remains arduous, especially for DC vaccines, and the prospect of using cell-based immunotherapy in the adjuvant setting or in combination with chemotherapy remains largely untested. Here, we used a comparative oncology approach to test the safety and potential efficacy of tumor RNA-loaded, CD40activated B cells in privately owned dogs presenting with non-Hodgkin's lymphoma (NHL), a clinical scenario that represents not only a major problem in veterinary medicine but also a bona fide spontaneous animal model for the human condition. When administered to NHL dogs in remission after induction chemotherapy, CD40-B cells electroporated ex vivo with autologous tumor RNA safely stimulated immunity in vivo. Although chemotherapy plus CD40-B vaccination did not improve time-to-progression or lymphoma-specific survival compared to dogs treated with chemotherapy alone, vaccination potentiated the effects of salvage therapy and improved the rate of durable second remissions as well as subsequent lymphoma-specific survival following salvage therapy. Several of these relapsed dogs are now long-term survivors and free of disease for more than a year. Overall, these clinical and immunological results suggest that cell-based CD40 cancer vaccination is safe and synergizes with chemotherapy to improve clinical outcome in canine NHL. More broadly, our findings underscore the unique value of clinical investigations in tumor-bearing companion animals.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16371687" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="917bc25365b202ad4d0bc4e71bc3a922" rel="nofollow" data-download="{"attachment_id":42508698,"asset_id":16371687,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42508698/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35503222" href="https://upenn.academia.edu/BethOverley">Beth Overley</a><script data-card-contents-for-user="35503222" type="text/json">{"id":35503222,"first_name":"Beth","last_name":"Overley","domain_name":"upenn","page_name":"BethOverley","display_name":"Beth Overley","profile_url":"https://upenn.academia.edu/BethOverley","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-16371687">+2</span><div class="hidden js-additional-users-16371687"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/KarinSorenmo">Karin Sorenmo</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://upenn.academia.edu/ErikaKrick">Erika Krick</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-16371687'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-16371687').html(); 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Manufacturing remains arduous, especially for DC vaccines, and the prospect of using cell-based immunotherapy in the adjuvant setting or in combination with chemotherapy remains largely untested. Here, we used a comparative oncology approach to test the safety and potential efficacy of tumor RNA-loaded, CD40activated B cells in privately owned dogs presenting with non-Hodgkin's lymphoma (NHL), a clinical scenario that represents not only a major problem in veterinary medicine but also a bona fide spontaneous animal model for the human condition. When administered to NHL dogs in remission after induction chemotherapy, CD40-B cells electroporated ex vivo with autologous tumor RNA safely stimulated immunity in vivo. Although chemotherapy plus CD40-B vaccination did not improve time-to-progression or lymphoma-specific survival compared to dogs treated with chemotherapy alone, vaccination potentiated the effects of salvage therapy and improved the rate of durable second remissions as well as subsequent lymphoma-specific survival following salvage therapy. Several of these relapsed dogs are now long-term survivors and free of disease for more than a year. Overall, these clinical and immunological results suggest that cell-based CD40 cancer vaccination is safe and synergizes with chemotherapy to improve clinical outcome in canine NHL. More broadly, our findings underscore the unique value of clinical investigations in tumor-bearing companion animals.","publication":"PLoS ONE","publication_with_fallback":"PLoS 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itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16371689/Outcome_and_toxicity_associated_with_a_dose_intensified_maintenance_free_CHOP_based_chemotherapy_protocol_in_canine_lymphoma_130_cases">Outcome and toxicity associated with a dose-intensified, maintenance-free CHOP-based chemotherapy protocol in canine lymphoma: 130 cases</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A dose-intensified/dose-dense chemotherapy protocol for canine lymphoma was designed and implemented at the Veterinary Hospital of the University of Pennsylvania. In this study, we describe the clinical characteristics, prognostic... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_16371689" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A dose-intensified/dose-dense chemotherapy protocol for canine lymphoma was designed and implemented at the Veterinary Hospital of the University of Pennsylvania. In this study, we describe the clinical characteristics, prognostic factors, efficacy and toxicity in 130 dogs treated with this protocol. The majority of the dogs had advanced stage disease (63.1% stage V) and sub-stage b (58.5%). The median time to progression (TTP) and lymphoma-specific survival were 219 and 323 days, respectively. These results are similar to previous less dose-intense protocols. Sub-stage was a significant negative prognostic factor for survival. The incidence of toxicity was high; 53.9 and 45% of the dogs needed dose reductions and treatment delays, respectively. Dogs that required dose reductions and treatment delays had significantly longer TTP and lymphoma-specific survival times. These results suggest that dose density is important, but likely relative, and needs to be adjusted according to the individual patient's toxicity for optimal outcome. recognized that a subset of patients would go into durable remissions (i.e. be cured); however, despite a high initial response rate (higher than 80% in most protocols), the majority of dogs would relapse and succumb to their lymphoma. Only 20-25% of the cases would be alive for 2 years or longer. The high response rates to relatively low dose-intense protocols indicate that canine lymphomas are uniquely chemotherapy sensitive tumours, and also suggest that further improvements, both in terms of remission rates and survival times, may be made through dose intensification. Results from human oncology trials, however, have been mixed; some studies have documented clinically significant improvements in outcome in patients treated with such doseintense/dose-dense protocols, whereas others have resulted in only marginal or no improvements in remission and survival durations. 21 -26 The inconsistent results from dose intensification may in part be due to the presence of cancer stem cells, a distinct</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16371689" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9ba045befec13a9c1f02cf0d70c01abb" rel="nofollow" data-download="{"attachment_id":42508685,"asset_id":16371689,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42508685/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35503222" href="https://upenn.academia.edu/BethOverley">Beth Overley</a><script data-card-contents-for-user="35503222" type="text/json">{"id":35503222,"first_name":"Beth","last_name":"Overley","domain_name":"upenn","page_name":"BethOverley","display_name":"Beth Overley","profile_url":"https://upenn.academia.edu/BethOverley","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-16371689">+2</span><div class="hidden js-additional-users-16371689"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/KarinSorenmo">Karin Sorenmo</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://upenn.academia.edu/ErikaKrick">Erika Krick</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-16371689'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-16371689').html(); 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In this study, we describe the clinical characteristics, prognostic factors, efficacy and toxicity in 130 dogs treated with this protocol. The majority of the dogs had advanced stage disease (63.1% stage V) and sub-stage b (58.5%). The median time to progression (TTP) and lymphoma-specific survival were 219 and 323 days, respectively. These results are similar to previous less dose-intense protocols. Sub-stage was a significant negative prognostic factor for survival. The incidence of toxicity was high; 53.9 and 45% of the dogs needed dose reductions and treatment delays, respectively. Dogs that required dose reductions and treatment delays had significantly longer TTP and lymphoma-specific survival times. These results suggest that dose density is important, but likely relative, and needs to be adjusted according to the individual patient's toxicity for optimal outcome. recognized that a subset of patients would go into durable remissions (i.e. be cured); however, despite a high initial response rate (higher than 80% in most protocols), the majority of dogs would relapse and succumb to their lymphoma. Only 20-25% of the cases would be alive for 2 years or longer. The high response rates to relatively low dose-intense protocols indicate that canine lymphomas are uniquely chemotherapy sensitive tumours, and also suggest that further improvements, both in terms of remission rates and survival times, may be made through dose intensification. Results from human oncology trials, however, have been mixed; some studies have documented clinically significant improvements in outcome in patients treated with such doseintense/dose-dense protocols, whereas others have resulted in only marginal or no improvements in remission and survival durations. 21 -26 The inconsistent results from dose intensification may in part be due to the presence of cancer stem cells, a distinct","publication":"Veterinary and Comparative Oncology","publication_with_fallback":"Veterinary and Comparative 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Oncology","url":"https://www.academia.edu/Documents/in/Veterinary_Comparative_Oncology"},{"id":1198588,"name":"Cyclophosphamide","url":"https://www.academia.edu/Documents/in/Cyclophosphamide"}],"publication_year":2000,"publication_year_with_fallback":2000,"paper_rank":null,"all_time_views":121,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21656864 coauthored" data-work_id="21656864" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21656864/A_double_blinded_placebo_controlled_pilot_study_to_examine_reduction_of_CD34_CD117_CD133_lymphoma_progenitor_cells_and_duration_of_remission_induced_by_neoadjuvant_valspodar_in_dogs_with_large_B_cell_lymphoma">A double blinded, placebo-controlled pilot study to examine reduction of CD34+/CD117+/CD133+ lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We previously described a population of lymphoid progenitor cells (LPCs) in canine B-cell lymphoma defined by retention of the early progenitor markers CD34 and CD117 and "slow proliferation" molecular signatures that persist in the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21656864" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We previously described a population of lymphoid progenitor cells (LPCs) in canine B-cell lymphoma defined by retention of the early progenitor markers CD34 and CD117 and "slow proliferation" molecular signatures that persist in the xenotransplantation setting. We examined whether valspodar, a selective inhibitor of the ATP binding cassette B1 transporter (ABCB1, a.k.a., p-glycoprotein/multidrug resistance protein-1) used in the neoadjuvant setting would sensitize LPCs to doxorubicin and extend the length of remission in dogs with therapy naïve large B-cell lymphoma. Twenty dogs were enrolled into a double-blinded, placebo controlled study where experimental and control groups received oral valspodar (7.5 mg/kg) or placebo, respectively, twice daily for five days followed by five treatments with doxorubicin 21 days apart with a reduction in the first dose to mitigate the potential side effects of ABCB1 inhibition. Lymph node and blood LPCs were quantified at diagnosis, on the fourth day of neoadjuvant period, and 1-week after the first chemotherapy + + +</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21656864" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="fa81bcdb5157a3c63b00fdae8f6a8880" rel="nofollow" data-download="{"attachment_id":42303553,"asset_id":21656864,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42303553/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42805049" href="https://independent.academia.edu/MichaelHenson2">Michael Henson</a><script data-card-contents-for-user="42805049" type="text/json">{"id":42805049,"first_name":"Michael","last_name":"Henson","domain_name":"independent","page_name":"MichaelHenson2","display_name":"Michael Henson","profile_url":"https://independent.academia.edu/MichaelHenson2","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-21656864">+2</span><div class="hidden js-additional-users-21656864"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/AntonellaBorgatti">Antonella Borgatti</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://upenn.academia.edu/ErikaKrick">Erika Krick</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-21656864'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-21656864').html(); 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container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_21656864 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="21656864"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 21656864; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=21656864]").text(description); $(".js-view-count-work_21656864").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21656864").removeClass('hidden') })</script></div></li></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21707691 coauthored" data-work_id="21707691" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21707691/Prognostic_significance_of_weight_changes_during_treatment_of_feline_lymphoma">Prognostic significance of weight changes during treatment of feline lymphoma</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The study purpose was to determine the prognostic significance of weight changes during feline lymphoma treatment. A secondary purpose was to compare weight changes according to baseline body weight, cell type and location. Records of 209... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21707691" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The study purpose was to determine the prognostic significance of weight changes during feline lymphoma treatment. A secondary purpose was to compare weight changes according to baseline body weight, cell type and location. Records of 209 cats treated for lymphoma with chemotherapy from 1995 to 2007 were evaluated. Signalment, cell type, lymphoma location, baseline body weight, weight during treatment, and outcome information were collected. Lymphoma specific survival (LSS) was compared according to baseline weight and weight changes during treatment. Weight change over time was compared according to cell type (small versus large), location (gastrointestinal versus non-gastrointestinal) and baseline weight. Cats with large cell lymphoma that lost !5% body weight at 1 month had significantly shorter LSS than those that gained or had stable weight (P ¼ 0.004). Percentage weight change over time differed significantly according to baseline weight group. These findings demonstrate the prognostic importance of weight loss in feline large cell lymphoma.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21707691" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="001b2d997b4979a57eb007a5afc1743a" rel="nofollow" data-download="{"attachment_id":42426582,"asset_id":21707691,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42426582/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42889260" href="https://upenn.academia.edu/ErikaKrick">Erika Krick</a><script data-card-contents-for-user="42889260" type="text/json">{"id":42889260,"first_name":"Erika","last_name":"Krick","domain_name":"upenn","page_name":"ErikaKrick","display_name":"Erika Krick","profile_url":"https://upenn.academia.edu/ErikaKrick","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-21707691">+1</span><div class="hidden js-additional-users-21707691"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/KarinSorenmo">Karin Sorenmo</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-21707691'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-21707691').html(); 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A secondary purpose was to compare weight changes according to baseline body weight, cell type and location. Records of 209 cats treated for lymphoma with chemotherapy from 1995 to 2007 were evaluated. Signalment, cell type, lymphoma location, baseline body weight, weight during treatment, and outcome information were collected. Lymphoma specific survival (LSS) was compared according to baseline weight and weight changes during treatment. Weight change over time was compared according to cell type (small versus large), location (gastrointestinal versus non-gastrointestinal) and baseline weight. Cats with large cell lymphoma that lost !5% body weight at 1 month had significantly shorter LSS than those that gained or had stable weight (P ¼ 0.004). Percentage weight change over time differed significantly according to baseline weight group. These findings demonstrate the prognostic importance of weight loss in feline large cell lymphoma.","publication":"Journal of Feline Medicine \u0026 Surgery","publication_with_fallback":"Journal of Feline Medicine \u0026 Surgery","downloadable_attachments":[{"id":42426582,"asset_id":21707691,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/42426582/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/42426582/Prognostic_significance_of_weight_change20160208-10346-12pav2i-libre.pdf?1454982793=\u0026response-content-disposition=attachment%3B+filename%3DPrognostic_significance_of_weight_change.pdf\u0026Expires=1740482108\u0026Signature=SD6qPphXzDnRZ4fWZJY3u6FvWAXQXxlmXcyy7Oe1-Vb7wcfuxMVQ99vFFdw5XWSITKU02sDbbQXCNjEi6k0C~px2xVFIdiH3ucAm2i4EkT5E~aoCHYsEfBNbCkD3GAFXGjLfsu6CnHKWvW5b3UXguTewbV8gsgOpH9cX4Z4sPHzHbdDq1nxz8muqKxLbeWRvVJ6aIjXurCpUixQSK3TTOzWM2FNffhX9dphnbFbt5YIVCQWoagm~K2d46f-rM80lCinjSUEljMQOrBByd9HnY7TFUZ~aMM73PxbiJgy78eNxHfYCbw5c6TJyzJywVpNn8iLBmedKtvDIdWfOifseQw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/42426582/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/42426582/mini_magick20190217-7239-9lfnkm.png?1550453791"}],"downloadable_attachments_with_full_thumbnails":[{"id":42426582,"asset_id":21707691,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/42426582/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/42426582/Prognostic_significance_of_weight_change20160208-10346-12pav2i-libre.pdf?1454982793=\u0026response-content-disposition=attachment%3B+filename%3DPrognostic_significance_of_weight_change.pdf\u0026Expires=1740482108\u0026Signature=SD6qPphXzDnRZ4fWZJY3u6FvWAXQXxlmXcyy7Oe1-Vb7wcfuxMVQ99vFFdw5XWSITKU02sDbbQXCNjEi6k0C~px2xVFIdiH3ucAm2i4EkT5E~aoCHYsEfBNbCkD3GAFXGjLfsu6CnHKWvW5b3UXguTewbV8gsgOpH9cX4Z4sPHzHbdDq1nxz8muqKxLbeWRvVJ6aIjXurCpUixQSK3TTOzWM2FNffhX9dphnbFbt5YIVCQWoagm~K2d46f-rM80lCinjSUEljMQOrBByd9HnY7TFUZ~aMM73PxbiJgy78eNxHfYCbw5c6TJyzJywVpNn8iLBmedKtvDIdWfOifseQw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/42426582/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/42426582/mini_magick20190217-7239-9lfnkm.png?1550453791"}],"has_pdf":true,"has_fulltext":true,"page_count":9,"ordered_authors":[{"id":42889260,"first_name":"Erika","last_name":"Krick","domain_name":"upenn","page_name":"ErikaKrick","display_name":"Erika Krick","profile_url":"https://upenn.academia.edu/ErikaKrick","photo":"/images/s65_no_pic.png"},{"id":35284923,"first_name":"Karin","last_name":"Sorenmo","domain_name":"independent","page_name":"KarinSorenmo","display_name":"Karin Sorenmo","profile_url":"https://independent.academia.edu/KarinSorenmo","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":8952,"name":"Breeding","url":"https://www.academia.edu/Documents/in/Breeding","nofollow":true},{"id":10610,"name":"Survival Analysis","url":"https://www.academia.edu/Documents/in/Survival_Analysis","nofollow":true},{"id":41088,"name":"Cats","url":"https://www.academia.edu/Documents/in/Cats","nofollow":true},{"id":55269,"name":"Lymphoma","url":"https://www.academia.edu/Documents/in/Lymphoma","nofollow":true},{"id":89805,"name":"Weight Loss","url":"https://www.academia.edu/Documents/in/Weight_Loss"},{"id":199732,"name":"Feline","url":"https://www.academia.edu/Documents/in/Feline"},{"id":272273,"name":"Philadelphia","url":"https://www.academia.edu/Documents/in/Philadelphia"},{"id":489727,"name":"Prognosis","url":"https://www.academia.edu/Documents/in/Prognosis"},{"id":564878,"name":"Body Weight","url":"https://www.academia.edu/Documents/in/Body_Weight"},{"id":644860,"name":"Veterinary Sciences","url":"https://www.academia.edu/Documents/in/Veterinary_Sciences"}],"publication_year":2011,"publication_year_with_fallback":2011,"paper_rank":null,"all_time_views":89,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_21707692" data-work_id="21707692" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/21707692/A_Phase_I_Clinical_Trial_of_Systemically_Delivered_NEMO_Binding_Domain_Peptide_in_Dogs_with_Spontaneous_Activated_B_Cell_like_Diffuse_Large_B_Cell_Lymphoma">A Phase I Clinical Trial of Systemically Delivered NEMO Binding Domain Peptide in Dogs with Spontaneous Activated B-Cell like Diffuse Large B-Cell Lymphoma</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Activated B-Cell (ABC) Diffuse Large B-Cell Lymphoma (DLBCL) is a common, aggressive and poorly chemoresponsive subtype of DLBCL, characterized by constitutive canonical NF-kB signaling. Inhibition of NF-kB signaling leads to apoptosis of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_21707692" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Activated B-Cell (ABC) Diffuse Large B-Cell Lymphoma (DLBCL) is a common, aggressive and poorly chemoresponsive subtype of DLBCL, characterized by constitutive canonical NF-kB signaling. Inhibition of NF-kB signaling leads to apoptosis of ABC-DLBCL cell lines, suggesting targeted disruption of this pathway may have therapeutic relevance. The selective IKK inhibitor, NEMO Binding Domain (NBD) peptide effectively blocks constitutive NF-kB activity and induces apoptosis in ABC-DLBCL cells in vitro. Here we used a comparative approach to determine the safety and efficacy of systemic NBD peptide to inhibit constitutive NF-kB signaling in privately owned dogs with spontaneous newly diagnosed or relapsed ABC-like DLBCL. Malignant lymph nodes biopsies were taken before and twenty-four hours after peptide administration to determine biological effects. Intravenous administration of ,2 mg/kg NBD peptide was safe and inhibited constitutive canonical NF-kB activity in 6/10 dogs. Reductions in mitotic index and Cyclin D expression also occurred in a subset of dogs 24 hours post peptide and in 3 dogs marked, therapeutically beneficial histopathological changes were identified. Mild, grade 1 toxicities were noted in 3 dogs at the time of peptide administration and one dog developed transient subclinical hepatopathy. Long term toxicities were not identified. Pharmacokinetic data suggested rapid uptake of peptide into tissues. No significant hematological or biochemical toxicities were identified. Overall the results from this phase I study indicate that systemic administration of NBD peptide is safe and effectively blocks constitutive NF-kB signaling and reduces malignant B cell proliferation in a subset of dogs with ABC-like DLBCL. These results have potential translational relevance for human ABC-DLBCL.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/21707692" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e0af96c83d2106a36b01029b37dd37f4" rel="nofollow" data-download="{"attachment_id":42426581,"asset_id":21707692,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42426581/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42889260" href="https://upenn.academia.edu/ErikaKrick">Erika Krick</a><script data-card-contents-for-user="42889260" type="text/json">{"id":42889260,"first_name":"Erika","last_name":"Krick","domain_name":"upenn","page_name":"ErikaKrick","display_name":"Erika Krick","profile_url":"https://upenn.academia.edu/ErikaKrick","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_21707692 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="21707692"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 21707692, container: ".js-paper-rank-work_21707692", }); 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$(".js-view-count[data-work-id=21707692]").text(description); $(".js-view-count-work_21707692").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_21707692").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="21707692"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="38831" rel="nofollow" href="https://www.academia.edu/Documents/in/Signal_Transduction">Signal Transduction</a>, <script data-card-contents-for-ri="38831" type="text/json">{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="52438" rel="nofollow" href="https://www.academia.edu/Documents/in/Dogs">Dogs</a>, <script data-card-contents-for-ri="52438" type="text/json">{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="123418" rel="nofollow" href="https://www.academia.edu/Documents/in/NF-kappa_B">NF-kappa B</a><script data-card-contents-for-ri="123418" type="text/json">{"id":123418,"name":"NF-kappa B","url":"https://www.academia.edu/Documents/in/NF-kappa_B","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=21707692]'), work: {"id":21707692,"title":"A Phase I Clinical Trial of Systemically Delivered NEMO Binding Domain Peptide in Dogs with Spontaneous Activated B-Cell like Diffuse Large B-Cell Lymphoma","created_at":"2016-02-08T17:51:28.075-08:00","owner_id":42889260,"url":"https://www.academia.edu/21707692/A_Phase_I_Clinical_Trial_of_Systemically_Delivered_NEMO_Binding_Domain_Peptide_in_Dogs_with_Spontaneous_Activated_B_Cell_like_Diffuse_Large_B_Cell_Lymphoma","slug":"A_Phase_I_Clinical_Trial_of_Systemically_Delivered_NEMO_Binding_Domain_Peptide_in_Dogs_with_Spontaneous_Activated_B_Cell_like_Diffuse_Large_B_Cell_Lymphoma","dom_id":"work_21707692","summary":"Activated B-Cell (ABC) Diffuse Large B-Cell Lymphoma (DLBCL) is a common, aggressive and poorly chemoresponsive subtype of DLBCL, characterized by constitutive canonical NF-kB signaling. Inhibition of NF-kB signaling leads to apoptosis of ABC-DLBCL cell lines, suggesting targeted disruption of this pathway may have therapeutic relevance. The selective IKK inhibitor, NEMO Binding Domain (NBD) peptide effectively blocks constitutive NF-kB activity and induces apoptosis in ABC-DLBCL cells in vitro. Here we used a comparative approach to determine the safety and efficacy of systemic NBD peptide to inhibit constitutive NF-kB signaling in privately owned dogs with spontaneous newly diagnosed or relapsed ABC-like DLBCL. Malignant lymph nodes biopsies were taken before and twenty-four hours after peptide administration to determine biological effects. Intravenous administration of ,2 mg/kg NBD peptide was safe and inhibited constitutive canonical NF-kB activity in 6/10 dogs. Reductions in mitotic index and Cyclin D expression also occurred in a subset of dogs 24 hours post peptide and in 3 dogs marked, therapeutically beneficial histopathological changes were identified. Mild, grade 1 toxicities were noted in 3 dogs at the time of peptide administration and one dog developed transient subclinical hepatopathy. Long term toxicities were not identified. Pharmacokinetic data suggested rapid uptake of peptide into tissues. No significant hematological or biochemical toxicities were identified. Overall the results from this phase I study indicate that systemic administration of NBD peptide is safe and effectively blocks constitutive NF-kB signaling and reduces malignant B cell proliferation in a subset of dogs with ABC-like DLBCL. These results have potential translational relevance for human ABC-DLBCL.","publication":"PLoS ONE","publication_with_fallback":"PLoS ONE","downloadable_attachments":[{"id":42426581,"asset_id":21707692,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/42426581/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/42426581/A_Phase_I_Clinical_Trial_of_Systemically20160208-1018-1ym3wyw-libre.pdf?1454982792=\u0026response-content-disposition=attachment%3B+filename%3DA_Phase_I_Clinical_Trial_of_Systemically.pdf\u0026Expires=1740604039\u0026Signature=GS569AO-G8OoD-ia8kNH4810Hm3P79ce67eOIzQ8AS5RatlDji1eGjpAzYLHSKUkWokJv2Lan7TjkImrnTB1DqCWTQJdQ9vaIMxZuWnDJ-0TpytJWd7wq2l2twMQdoFmgqKsB6x~ZvlCwF3ZshLJja99gnoT0iQ6Yd9RXwCBHfCAz~A1M79ETztAJFVGfnoTcAAfS2qB2KJtK7~1hOD2NnsmCdDsDEWy9QOxogzHlc2KiDDLPeTIHFtP9PzPTj2ZWhQyJCktSIErnzQtb6qsNhWAwD6ZERcM8r66I-rrweuFlrWC4ckcp~3PjmYGiDFVunvKxuaGtp3rmt-azYE3QA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/42426581/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/42426581/mini_magick20220704-3284-1vwjj4v.png?1656988057"}],"downloadable_attachments_with_full_thumbnails":[{"id":42426581,"asset_id":21707692,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/42426581/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/42426581/A_Phase_I_Clinical_Trial_of_Systemically20160208-1018-1ym3wyw-libre.pdf?1454982792=\u0026response-content-disposition=attachment%3B+filename%3DA_Phase_I_Clinical_Trial_of_Systemically.pdf\u0026Expires=1740604039\u0026Signature=GS569AO-G8OoD-ia8kNH4810Hm3P79ce67eOIzQ8AS5RatlDji1eGjpAzYLHSKUkWokJv2Lan7TjkImrnTB1DqCWTQJdQ9vaIMxZuWnDJ-0TpytJWd7wq2l2twMQdoFmgqKsB6x~ZvlCwF3ZshLJja99gnoT0iQ6Yd9RXwCBHfCAz~A1M79ETztAJFVGfnoTcAAfS2qB2KJtK7~1hOD2NnsmCdDsDEWy9QOxogzHlc2KiDDLPeTIHFtP9PzPTj2ZWhQyJCktSIErnzQtb6qsNhWAwD6ZERcM8r66I-rrweuFlrWC4ckcp~3PjmYGiDFVunvKxuaGtp3rmt-azYE3QA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/42426581/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/42426581/mini_magick20220704-3284-1vwjj4v.png?1656988057"}],"has_pdf":true,"has_fulltext":true,"page_count":10,"ordered_authors":[{"id":42889260,"first_name":"Erika","last_name":"Krick","domain_name":"upenn","page_name":"ErikaKrick","display_name":"Erika Krick","profile_url":"https://upenn.academia.edu/ErikaKrick","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary","nofollow":true},{"id":38831,"name":"Signal Transduction","url":"https://www.academia.edu/Documents/in/Signal_Transduction","nofollow":true},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs","nofollow":true},{"id":123418,"name":"NF-kappa B","url":"https://www.academia.edu/Documents/in/NF-kappa_B","nofollow":true},{"id":151086,"name":"Peptides","url":"https://www.academia.edu/Documents/in/Peptides"},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":541328,"name":"Diffuse Large B-Cell Lymphoma","url":"https://www.academia.edu/Documents/in/Diffuse_Large_B-Cell_Lymphoma"},{"id":678853,"name":"B Lymphocytes","url":"https://www.academia.edu/Documents/in/B_Lymphocytes"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents"}],"publication_year":2014,"publication_year_with_fallback":2014,"paper_rank":null,"all_time_views":28,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6433584" data-work_id="6433584" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6433584/Molecular_biology_genetics_and_biotechnology_Bacterial_diversity_associated_with_feeding_dry_forage_at_different_dietary_concentrations_in_the_rumen_contents_of_Mehshana_buffalo_Bubalus_bubalis_using_16S_pyrotags">Molecular biology, genetics and biotechnology Bacterial diversity associated with feeding dry forage at different dietary concentrations in the rumen contents of Mehshana buffalo (Bubalus bubalis) using 16S pyrotags</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Pyrosequencing of 16S rRNA gene targeting bacteria was applied to identify diet-induced shifts in the microbiome of both solid and liquid ruminal fractions retrieved from water buffalo fed different diets. The depth of coverage of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6433584" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Pyrosequencing of 16S rRNA gene targeting bacteria was applied to identify diet-induced shifts in the microbiome of both solid and liquid ruminal fractions retrieved from water buffalo fed different diets. The depth of coverage of metabolically active bacteria in a community using different primer pairs was also investigated. To assess reproducibility, animal to animal variation was considered in all phylogenetic and community comparisons. The experiment included four non-lactating water buffaloes fed three different diets for six weeks each; diets were M1 (50% concentrate: 50% dry roughage), M2 (25% concentrate: 75% dry roughage) and M3 (100% dry roughage). A total of 333, 851 pyrotags were analyzed in this study. Phylogenetic analysis revealed significant differences in the rumen microbiome mediated by primer and diet (P < 0.05). Differences in community composition due to primer, diet, fraction and animal were compared using unweighted and weighted UniFrac analysis. Clustering of communities was largely explained by primer differences in both weighted and unweighted UniFrac analyses (P < 0.001). In the weighted analysis, communities clustered by diets (P < 0.05) and fractions (P < 0.08) while no inter-animal variation was observed. The identified repertoire of bacterial populations was dependent on the primer pair, as targeting the V4eV5 region resulted in greater diversity profiles of the microbiome. Within each primer pair, dietary changes altered the community composition with noticeable shifts at genus level. Genera such as Ruminococcus and Fibrobacter (P < 0.05) were higher in abundance on M3 diet while Prevotella dominated (P < 0.05) on M1 diet.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6433584" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="47d5a02d175f1e92694409a4abe8d5f3" rel="nofollow" data-download="{"attachment_id":33232954,"asset_id":6433584,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/33232954/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_6433584 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6433584"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6433584, container: ".js-paper-rank-work_6433584", }); 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In the weighted analysis, communities clustered by diets (P \u003c 0.05) and fractions (P \u003c 0.08) while no inter-animal variation was observed. The identified repertoire of bacterial populations was dependent on the primer pair, as targeting the V4eV5 region resulted in greater diversity profiles of the microbiome. Within each primer pair, dietary changes altered the community composition with noticeable shifts at genus level. 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Ewes of similar... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6433586" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Effects of supplementing willow stem cuttings to ewes grazing drought pastures upon plasma amino acid (AA) concentrations was studied on Massey University's Riverside Farm, near Masteron, on the East Coast of New Zealand. Ewes of similar age and weight (i.e., 59.0 ± 2.22 kg) were assigned to two groups of 7 each, either with (supplemented) or without (control) supplementation of willow, and experimental grazing was carried for 10 weeks from early February until mid April of 2005. Live weight (LW) was recorded fortnightly and body condition score (BCS) was monthly. Blood samples for quantification of plasma amino acids were collected at week 5 and 10. Both groups had a similar pre-grazing pasture mass (i.e., 2000 kg of dry matter/ha) and dead matter content (0.80) with the diet selected by the ewes containing a metabolisable energy (ME) of 8.3 MJ/kg DM, which is typical of drought conditions. The willow was readily eaten, with intake averaging 0.26 kg DM/ewe/d. Willow was of higher ME content than short drought pasture (i.e., 10.1 versus 8.4 MJ/kg DM) and contained condensed tannins at 40.8 ± 1.97 g/kg DM. Both groups of ewes lost live weight at about 50 g/d. Plasma concentration of 3-methyl histidine (88 versus 127 mol/L) at week 5 and Abbreviations: AA, amino acid; BCAA, branched chain AA; BCS, body condition score; DM, dry matter; DOMD, digestible OM digestibility; EAA, essential amino acid; HPLC, high performance liquid chromatography; LW, live weight; 3-MTH, 3-methyl histidine; ME, metabolisable energy; aNDF, neutral detergent fibre; NE, net energy; NEAA, non-essential amino acid; OM, organic matter. non-essential amino acids (1082 versus 1417 mol/L) at week 5 and (1155 versus 1324 mol/L) at week 10, were substantially lower (P<0.05) in willow supplemented versus control ewes, indicating that willow supplementation reduced catabolism of body proteins in ewes under drought feeding conditions.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6433586" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="525fa6f731f21d8d4b9d6eb44c71f262" rel="nofollow" data-download="{"attachment_id":48869681,"asset_id":6433586,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48869681/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_6433586 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6433586"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6433586, container: ".js-paper-rank-work_6433586", }); 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Ewes of similar age and weight (i.e., 59.0 ± 2.22 kg) were assigned to two groups of 7 each, either with (supplemented) or without (control) supplementation of willow, and experimental grazing was carried for 10 weeks from early February until mid April of 2005. Live weight (LW) was recorded fortnightly and body condition score (BCS) was monthly. Blood samples for quantification of plasma amino acids were collected at week 5 and 10. Both groups had a similar pre-grazing pasture mass (i.e., 2000 kg of dry matter/ha) and dead matter content (0.80) with the diet selected by the ewes containing a metabolisable energy (ME) of 8.3 MJ/kg DM, which is typical of drought conditions. The willow was readily eaten, with intake averaging 0.26 kg DM/ewe/d. Willow was of higher ME content than short drought pasture (i.e., 10.1 versus 8.4 MJ/kg DM) and contained condensed tannins at 40.8 ± 1.97 g/kg DM. Both groups of ewes lost live weight at about 50 g/d. Plasma concentration of 3-methyl histidine (88 versus 127 mol/L) at week 5 and Abbreviations: AA, amino acid; BCAA, branched chain AA; BCS, body condition score; DM, dry matter; DOMD, digestible OM digestibility; EAA, essential amino acid; HPLC, high performance liquid chromatography; LW, live weight; 3-MTH, 3-methyl histidine; ME, metabolisable energy; aNDF, neutral detergent fibre; NE, net energy; NEAA, non-essential amino acid; OM, organic matter. non-essential amino acids (1082 versus 1417 mol/L) at week 5 and (1155 versus 1324 mol/L) at week 10, were substantially lower (P\u003c0.05) in willow supplemented versus control ewes, indicating that willow supplementation reduced catabolism of body proteins in ewes under drought feeding conditions.","publication":"Animal Feed Science and Technology","publication_with_fallback":"Animal Feed Science and Technology","downloadable_attachments":[{"id":48869681,"asset_id":6433586,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/48869681/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/48869681/Effect_of_willow_supplementation_upon_pl20160915-24297-1l5nrh5-libre.pdf?1473991295=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_willow_supplementation_upon_pl.pdf\u0026Expires=1740604039\u0026Signature=VOBMhdAD3~~beBMuoErNL1-yVW5SlGexTXGvOm7RcUPCLmfvJdbf1RZ9-44i0NSRIFDwLCZZ2aVBea7BPTxwjk0Y5vGpUvfe0Ddj9YzGD8PxtL0LVbyiKg0RWp~IaeQVUJWY8Y4Hx4-NViPhg1GS369hq7yr4GPWKBQiAR4Dyido74~nijmVrDoSZ6ZSWEGJmbPGoIuhRJRg-KAoEXVsau0QeZBbzUZna9KeorqqYXTCoSgg36S2dIO9rGmEhw2tyeaF3f5EHu6GRCsLiM30ONQM8GVrHaJBawlKX9Xy2WxwVAxZDFWkA~j3mFPqg6XdnLprTB~I4L1TsBPs35~n8w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/48869681/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/48869681/mini_magick20190202-27348-figcii.png?1549117002"}],"downloadable_attachments_with_full_thumbnails":[{"id":48869681,"asset_id":6433586,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/48869681/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/48869681/Effect_of_willow_supplementation_upon_pl20160915-24297-1l5nrh5-libre.pdf?1473991295=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_willow_supplementation_upon_pl.pdf\u0026Expires=1740604039\u0026Signature=VOBMhdAD3~~beBMuoErNL1-yVW5SlGexTXGvOm7RcUPCLmfvJdbf1RZ9-44i0NSRIFDwLCZZ2aVBea7BPTxwjk0Y5vGpUvfe0Ddj9YzGD8PxtL0LVbyiKg0RWp~IaeQVUJWY8Y4Hx4-NViPhg1GS369hq7yr4GPWKBQiAR4Dyido74~nijmVrDoSZ6ZSWEGJmbPGoIuhRJRg-KAoEXVsau0QeZBbzUZna9KeorqqYXTCoSgg36S2dIO9rGmEhw2tyeaF3f5EHu6GRCsLiM30ONQM8GVrHaJBawlKX9Xy2WxwVAxZDFWkA~j3mFPqg6XdnLprTB~I4L1TsBPs35~n8w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/48869681/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/48869681/mini_magick20190202-27348-figcii.png?1549117002"}],"has_pdf":true,"has_fulltext":true,"page_count":10,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production","nofollow":true},{"id":48127,"name":"Concentration","url":"https://www.academia.edu/Documents/in/Concentration","nofollow":true},{"id":75847,"name":"HPLC","url":"https://www.academia.edu/Documents/in/HPLC","nofollow":true},{"id":103298,"name":"Blood sampling","url":"https://www.academia.edu/Documents/in/Blood_sampling","nofollow":true},{"id":116108,"name":"New Zealand","url":"https://www.academia.edu/Documents/in/New_Zealand"},{"id":225787,"name":"High Performance Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography"},{"id":586220,"name":"Body Condition Score","url":"https://www.academia.edu/Documents/in/Body_Condition_Score"},{"id":953277,"name":"Dry Matter","url":"https://www.academia.edu/Documents/in/Dry_Matter"},{"id":2369223,"name":"Nutritive Value","url":"https://www.academia.edu/Documents/in/Nutritive_Value"},{"id":2412287,"name":"Diet Selection","url":"https://www.academia.edu/Documents/in/Diet_Selection"}],"publication_year":2009,"publication_year_with_fallback":2009,"paper_rank":null,"all_time_views":52,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6433587" data-work_id="6433587" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6433587/Willow_fodder_blocks_An_alternate_forage_to_low_quality_pasture_for_mating_ewes_during_drought">Willow fodder blocks—An alternate forage to low quality pasture for mating ewes during drought</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Two grazing experiments were conducted in the East Coast region of the North Island of NZ with ewes grazing on willow fodder blocks or control grass-based pastures during mating. Experiment I was conducted for 86 days with three... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6433587" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Two grazing experiments were conducted in the East Coast region of the North Island of NZ with ewes grazing on willow fodder blocks or control grass-based pastures during mating. Experiment I was conducted for 86 days with three treatments; short drought pasture (5-7 cm), long drought pasture (25-30 cm) and full access to willow fodder blocks which also contained long pasture (25-30 cm). Experiment II was conducted for 35 days with two treatments; control pasture and full access to willow fodder blocks. All ewe groups were combined at the end of mating and grazed on pastures until lambing and weaning. Live weight (LW) was recorded fortnightly during experimental grazing, body condition score (BCS) was scored monthly, and reproductive data was recorded at ultra-sound pregnancy scanning, lambing, docking and weaning. In Experiment I, short drought pasture and long drought pasture contained, respectively, a metabolisable energy (ME) of 8.2 and 9.6 MJ/kg dry matter (DM) and only traces of condensed tannins (CT). Herbage in the willow fodder blocks was intermediate in ME to short drought pasture and long drought pasture, whilst trees in willow fodder blocks contained 38 g of CT/kg DM and had a ME of approximately 10 MJ/kg DM. Willow fodder block herbage was consistently of higher legume content than drought pasture of similar mass. Ewes grazing short drought pasture lost 40 g/day of LW and had a low reproductive rate, as would occur in a drought situation, whilst ewes grazing long drought pasture gained LW and had a higher reproductive 241 rate. Ewes grazing willow fodder blocks maintained LW and their reproductive performance was intermediate to ewes mated on long drought pasture and short drought pasture. Calculated intakes of DM, ME and crude protein (CP) were low for ewes grazing short drought pasture and higher and similar for the other two groups, while intake of secondary compounds was much higher for ewes grazing willow fodder blocks. In Experiment II, ewes mated on willow fodder blocks had lower LW gain than ewes mated on control pasture, but reproductive performance was similar for both groups. Willow fodder blocks are a useful source of supplementary feed during droughts, but the grazing management that optimizes animal performance needs further research.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6433587" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8be376be6ba867311e9b4153747e5dca" rel="nofollow" data-download="{"attachment_id":48869694,"asset_id":6433587,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48869694/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_6433587 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6433587"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6433587, container: ".js-paper-rank-work_6433587", }); 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$(".js-view-count[data-work-id=6433587]").text(description); $(".js-view-count-work_6433587").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_6433587").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="6433587"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">7</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="7020" rel="nofollow" href="https://www.academia.edu/Documents/in/Grazing_and_Range_Management">Grazing and Range Management</a>, <script data-card-contents-for-ri="7020" type="text/json">{"id":7020,"name":"Grazing and Range Management","url":"https://www.academia.edu/Documents/in/Grazing_and_Range_Management","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="29980" rel="nofollow" href="https://www.academia.edu/Documents/in/Animal_Production">Animal Production</a>, <script data-card-contents-for-ri="29980" type="text/json">{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="127214" rel="nofollow" href="https://www.academia.edu/Documents/in/Ultra_Sound">Ultra Sound</a>, <script data-card-contents-for-ri="127214" type="text/json">{"id":127214,"name":"Ultra Sound","url":"https://www.academia.edu/Documents/in/Ultra_Sound","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="586220" rel="nofollow" href="https://www.academia.edu/Documents/in/Body_Condition_Score">Body Condition Score</a><script data-card-contents-for-ri="586220" type="text/json">{"id":586220,"name":"Body Condition Score","url":"https://www.academia.edu/Documents/in/Body_Condition_Score","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=6433587]'), work: {"id":6433587,"title":"Willow fodder blocks—An alternate forage to low quality pasture for mating ewes during drought","created_at":"2014-03-16T11:05:54.696-07:00","owner_id":10149035,"url":"https://www.academia.edu/6433587/Willow_fodder_blocks_An_alternate_forage_to_low_quality_pasture_for_mating_ewes_during_drought","slug":"Willow_fodder_blocks_An_alternate_forage_to_low_quality_pasture_for_mating_ewes_during_drought","dom_id":"work_6433587","summary":"Two grazing experiments were conducted in the East Coast region of the North Island of NZ with ewes grazing on willow fodder blocks or control grass-based pastures during mating. Experiment I was conducted for 86 days with three treatments; short drought pasture (5-7 cm), long drought pasture (25-30 cm) and full access to willow fodder blocks which also contained long pasture (25-30 cm). Experiment II was conducted for 35 days with two treatments; control pasture and full access to willow fodder blocks. All ewe groups were combined at the end of mating and grazed on pastures until lambing and weaning. Live weight (LW) was recorded fortnightly during experimental grazing, body condition score (BCS) was scored monthly, and reproductive data was recorded at ultra-sound pregnancy scanning, lambing, docking and weaning. In Experiment I, short drought pasture and long drought pasture contained, respectively, a metabolisable energy (ME) of 8.2 and 9.6 MJ/kg dry matter (DM) and only traces of condensed tannins (CT). Herbage in the willow fodder blocks was intermediate in ME to short drought pasture and long drought pasture, whilst trees in willow fodder blocks contained 38 g of CT/kg DM and had a ME of approximately 10 MJ/kg DM. Willow fodder block herbage was consistently of higher legume content than drought pasture of similar mass. Ewes grazing short drought pasture lost 40 g/day of LW and had a low reproductive rate, as would occur in a drought situation, whilst ewes grazing long drought pasture gained LW and had a higher reproductive 241 rate. Ewes grazing willow fodder blocks maintained LW and their reproductive performance was intermediate to ewes mated on long drought pasture and short drought pasture. Calculated intakes of DM, ME and crude protein (CP) were low for ewes grazing short drought pasture and higher and similar for the other two groups, while intake of secondary compounds was much higher for ewes grazing willow fodder blocks. In Experiment II, ewes mated on willow fodder blocks had lower LW gain than ewes mated on control pasture, but reproductive performance was similar for both groups. Willow fodder blocks are a useful source of supplementary feed during droughts, but the grazing management that optimizes animal performance needs further research.","publication":"Animal Feed Science and Technology","publication_with_fallback":"Animal Feed Science and Technology","downloadable_attachments":[{"id":48869694,"asset_id":6433587,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/48869694/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/48869694/j.anifeedsci.2006.04.00620160915-24302-61ypz5-libre.pdf?1473991291=\u0026response-content-disposition=attachment%3B+filename%3DWillow_fodder_blocks_An_alternate_forage.pdf\u0026Expires=1740604039\u0026Signature=QwxP6TIaZMmMsJSicPXRYswxovePIepHfTSIKOTjTS2-NldNHCPKcRgOyt55Sd2c9RcTa-58f0xY2cuMQfTZAMgzP6u~Sp1WkV~j6kxLLflK3~A0JPWH-1QIUI75K9u823vwWxiOmMklWBa5-9Ktp3cUjC7kM-THC-EK5o3Sv-5gdUukpBZt59ge~EOXPJeynxXaSDztN5~o-Apb-gkPOMMrvBi9ykv94e2IVZ~~vxxnHqUMKYE6pu82AuwblmerSPtDLychLJcMPwJiCFKehSAWoEq7aBEX84VVnHMLzIWLv5bjCjRGncgf5Xr8Z7EuIKW6emADv1aq33kRNBS0Hg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/48869694/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/48869694/mini_magick20190202-10828-19pw63h.png?1549117034"}],"downloadable_attachments_with_full_thumbnails":[{"id":48869694,"asset_id":6433587,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/48869694/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/48869694/j.anifeedsci.2006.04.00620160915-24302-61ypz5-libre.pdf?1473991291=\u0026response-content-disposition=attachment%3B+filename%3DWillow_fodder_blocks_An_alternate_forage.pdf\u0026Expires=1740604039\u0026Signature=QwxP6TIaZMmMsJSicPXRYswxovePIepHfTSIKOTjTS2-NldNHCPKcRgOyt55Sd2c9RcTa-58f0xY2cuMQfTZAMgzP6u~Sp1WkV~j6kxLLflK3~A0JPWH-1QIUI75K9u823vwWxiOmMklWBa5-9Ktp3cUjC7kM-THC-EK5o3Sv-5gdUukpBZt59ge~EOXPJeynxXaSDztN5~o-Apb-gkPOMMrvBi9ykv94e2IVZ~~vxxnHqUMKYE6pu82AuwblmerSPtDLychLJcMPwJiCFKehSAWoEq7aBEX84VVnHMLzIWLv5bjCjRGncgf5Xr8Z7EuIKW6emADv1aq33kRNBS0Hg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/48869694/download_file?st=MTc0MDYwMDQzOSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/48869694/mini_magick20190202-10828-19pw63h.png?1549117034"}],"has_pdf":true,"has_fulltext":true,"page_count":19,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":7020,"name":"Grazing and Range Management","url":"https://www.academia.edu/Documents/in/Grazing_and_Range_Management","nofollow":true},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production","nofollow":true},{"id":127214,"name":"Ultra Sound","url":"https://www.academia.edu/Documents/in/Ultra_Sound","nofollow":true},{"id":586220,"name":"Body Condition Score","url":"https://www.academia.edu/Documents/in/Body_Condition_Score","nofollow":true},{"id":645803,"name":"Crude Protein","url":"https://www.academia.edu/Documents/in/Crude_Protein"},{"id":953277,"name":"Dry Matter","url":"https://www.academia.edu/Documents/in/Dry_Matter"},{"id":2277123,"name":"Reproductive Performance","url":"https://www.academia.edu/Documents/in/Reproductive_Performance"}],"publication_year":2007,"publication_year_with_fallback":2007,"paper_rank":null,"all_time_views":31,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6433588" data-work_id="6433588" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6433588/Effects_on_ewe_reproduction_of_grazing_willow_fodder_blocks_during_drought">Effects on ewe reproduction of grazing willow fodder blocks during drought</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A grazing experiment was conducted in the summer/autumn of 2003 to determine the effect of grazing on willow fodder blocks at 6000 stems/ha during mating, relative to control ewes grazed on drought pasture, upon ewe production and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6433588" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A grazing experiment was conducted in the summer/autumn of 2003 to determine the effect of grazing on willow fodder blocks at 6000 stems/ha during mating, relative to control ewes grazed on drought pasture, upon ewe production and reproduction. The fodder blocks contained a mixture of herbage and small trees. Grazing occurred over 10 weeks, from 19 February including three cycles of mating, with four groups of 100 ewes, comprising short drought pasture typical of drought pasture, long drought pasture typical of the pasture growing in the willow fodder blocks, short drought pasture with restricted access to willow fodder blocks (restricted access) and full access to willow fodder blocks (fenced on the willow fodder blocks all the time; full access). After mating, the four groups were joined and managed as one group until weaning in late November 2003. Ewe live weight (LW) and body condition score (BCS) change and reproductive rate at foetal ultra-sound scanning, lambing, docking and weaning were measured. Ewe wool production and staple length were measured at weaning. Short drought pasture had a pre-grazing mass of 1639 kg dry matter (DM)/ha with a dead matter content of 60%; typical of drought conditions. Herbage in the willow fodder blocks was similar to both control drought pastures (short and long) in nutritive value, with an organic matter digestibility (OMD) of 0.50. Tree yields were low in the fodder blocks, but they had higher concentrations of all secondary compounds, including 30 g condensed tannin (CT)/kg DM and OMD was higher, at 0.72. CT concentration was higher in the fodder block herbage than in short and long control drought pasture (5.0 g/kg versus 2.5 g/kg DM). Substantial LW loss occurred in the short control group (101 g/day), and reproductive rate was low, as would occur in severe drought conditions. Full access to fodder blocks lowered LW loss to 40 g/day and increased reproductive rate by approximately 20% units, with more ewes giving birth to twin lambs. Restricted access ewes had a low reproductive rate, similar to the short control group. Reproductive rate in full access treatment was slightly higher than in the long control group, despite similar calculated DM intakes in both groups. Calculated crude protein and CT intakes were higher for full access ewes than for any other groups, due to contributions from both the herbage and the trees; this may have increased the flow of undegradable dietary protein (UDP) to the small intestine and so have contributed to the increased fecundity of this group. Full access to willow fodder blocks proved beneficial in increasing ewe reproductive rate. However, both pasture and trees need to be managed as a tree/pasture system in order to produce herbage of higher nutritive value and more efficiently utilise willow fodder blocks as a supplementary feed.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6433588" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9e1c35a530b45742cfd102ccce1ce534" rel="nofollow" data-download="{"attachment_id":48869677,"asset_id":6433588,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48869677/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_6433588 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6433588"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6433588, container: ".js-paper-rank-work_6433588", }); 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The fodder blocks contained a mixture of herbage and small trees. Grazing occurred over 10 weeks, from 19 February including three cycles of mating, with four groups of 100 ewes, comprising short drought pasture typical of drought pasture, long drought pasture typical of the pasture growing in the willow fodder blocks, short drought pasture with restricted access to willow fodder blocks (restricted access) and full access to willow fodder blocks (fenced on the willow fodder blocks all the time; full access). After mating, the four groups were joined and managed as one group until weaning in late November 2003. Ewe live weight (LW) and body condition score (BCS) change and reproductive rate at foetal ultra-sound scanning, lambing, docking and weaning were measured. Ewe wool production and staple length were measured at weaning. Short drought pasture had a pre-grazing mass of 1639 kg dry matter (DM)/ha with a dead matter content of 60%; typical of drought conditions. Herbage in the willow fodder blocks was similar to both control drought pastures (short and long) in nutritive value, with an organic matter digestibility (OMD) of 0.50. Tree yields were low in the fodder blocks, but they had higher concentrations of all secondary compounds, including 30 g condensed tannin (CT)/kg DM and OMD was higher, at 0.72. CT concentration was higher in the fodder block herbage than in short and long control drought pasture (5.0 g/kg versus 2.5 g/kg DM). Substantial LW loss occurred in the short control group (101 g/day), and reproductive rate was low, as would occur in severe drought conditions. Full access to fodder blocks lowered LW loss to 40 g/day and increased reproductive rate by approximately 20% units, with more ewes giving birth to twin lambs. Restricted access ewes had a low reproductive rate, similar to the short control group. Reproductive rate in full access treatment was slightly higher than in the long control group, despite similar calculated DM intakes in both groups. Calculated crude protein and CT intakes were higher for full access ewes than for any other groups, due to contributions from both the herbage and the trees; this may have increased the flow of undegradable dietary protein (UDP) to the small intestine and so have contributed to the increased fecundity of this group. Full access to willow fodder blocks proved beneficial in increasing ewe reproductive rate. However, both pasture and trees need to be managed as a tree/pasture system in order to produce herbage of higher nutritive value and more efficiently utilise willow fodder blocks as a supplementary feed.","publication":"Animal Feed Science and Technology","publication_with_fallback":"Animal Feed Science and Technology","downloadable_attachments":[{"id":48869677,"asset_id":6433588,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/48869677/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/48869677/Effects_on_ewe_reproduction_of_grazing_w20160915-14917-dcxrnf-libre.pdf?1473991297=\u0026response-content-disposition=attachment%3B+filename%3DEffects_on_ewe_reproduction_of_grazing_w.pdf\u0026Expires=1740604039\u0026Signature=bOucfgUi2d4S1EvI9QWmxfvjcaYqKmJFOsXcr7rSbnjsZ5LbdiuRQkTney~A9JHXySA5PDgvXrmBxEZwBWLyI1lRMSqt4jVqQYacD5ImM74-jdEG3c0-Ru4lTXReKwMovXN731YVF6czWfuCyhDl~4~0LS740KEOpHriPR-WL94TiJvjMZrzxIYjbMFEuFrQxfhKdZUllLTU0-tSMhIAmgaSmxDU1LncRotxQO3JoMQZMglMQSeitaAoWVdYN08ExwWwiSRJpmTKpcwjnYPL0IwXS0GVg9P0AqpWTPkf0mQgM0xy9cg36ft0IobdjVdh-AaTTQz1bwB9NeAybjWsXA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/48869677/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/48869677/mini_magick20190202-17849-emccwv.png?1549117006"}],"downloadable_attachments_with_full_thumbnails":[{"id":48869677,"asset_id":6433588,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/48869677/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/48869677/Effects_on_ewe_reproduction_of_grazing_w20160915-14917-dcxrnf-libre.pdf?1473991297=\u0026response-content-disposition=attachment%3B+filename%3DEffects_on_ewe_reproduction_of_grazing_w.pdf\u0026Expires=1740604040\u0026Signature=MQ0Xo8jBpoSZE3d5QYMFZuRr-qv4Pwui2-owcQPeqxQbV44nl9m0SUKQALklKJJqaWWqSvqYqcbvtEkW~j0~gzXzTPS2eISrV0NWGdh4sjzmAxVL~0AdoxmepYkFTugxyOgwX0PTCd6OeNAG4ejIczNywC2KRuxLONwIltmDzkMm6D92V6GRlSaKgESEB9CP7k5CoErJLC~VobA1Q3J0yF6YXwV136ZqkOv99OdaqJFq~udZ6yW7-QmOxrAaPjNcI1BbM55c5SNNVy~pBUoXeqTmV8vWFmk-r3A7iYZfKihsZGybmqqMDPK1QxotMLW9iA9aQZuxxgp7je-9TziDxA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/48869677/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/48869677/mini_magick20190202-17849-emccwv.png?1549117006"}],"has_pdf":true,"has_fulltext":true,"page_count":18,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production","nofollow":true},{"id":127214,"name":"Ultra Sound","url":"https://www.academia.edu/Documents/in/Ultra_Sound","nofollow":true},{"id":440091,"name":"Organic Matter Digestibility","url":"https://www.academia.edu/Documents/in/Organic_Matter_Digestibility","nofollow":true},{"id":586220,"name":"Body Condition Score","url":"https://www.academia.edu/Documents/in/Body_Condition_Score","nofollow":true},{"id":645803,"name":"Crude Protein","url":"https://www.academia.edu/Documents/in/Crude_Protein"},{"id":900018,"name":"Dietary Protein","url":"https://www.academia.edu/Documents/in/Dietary_Protein"},{"id":953277,"name":"Dry Matter","url":"https://www.academia.edu/Documents/in/Dry_Matter"},{"id":1631043,"name":"Control Group","url":"https://www.academia.edu/Documents/in/Control_Group"},{"id":1647723,"name":"Small Intestine","url":"https://www.academia.edu/Documents/in/Small_Intestine"},{"id":2369223,"name":"Nutritive Value","url":"https://www.academia.edu/Documents/in/Nutritive_Value"}],"publication_year":2005,"publication_year_with_fallback":2005,"paper_rank":null,"all_time_views":6,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_6433589" data-work_id="6433589" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/6433589/Rumen_Bacterial_Diversity_Dynamics_Associated_with_Changing_from_Bermudagrass_Hay_to_Grazed_Winter_Wheat_Diets">Rumen Bacterial Diversity Dynamics Associated with Changing from Bermudagrass Hay to Grazed Winter Wheat Diets</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Rumen bacterial communities in forage-fed and grazing cattle continually adapt to a wide range of changing dietary composition, nutrient density, and environmental conditions. We hypothesized that very distinct community assemblages would... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6433589" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Rumen bacterial communities in forage-fed and grazing cattle continually adapt to a wide range of changing dietary composition, nutrient density, and environmental conditions. We hypothesized that very distinct community assemblages would develop between the fiber and liquid fractions of rumen contents in animals transitioned from bermudagrass hay diet to a grazed wheat diet. To address this hypothesis, we designed an experiment utilizing a 16S-based bTEFAP pyrosequencing technique to characterize and elucidate changes in bacterial diversity among the fiber and liquid rumen fractions and whole rumen contents of 14 (Angus × Hereford) ruminally cannulated steers sequentially fed bermudagrass hay (Cynodon dactylon; 34 days) and grazing wheat forage (28 days). Bermudagrass hay was a conserved C4 perennial grass lower in protein and higher in fiber (11% and 67%, respectively) content than grazed winter wheat (Triticum aestivum), a C3 annual grass with higher protein (20%) and a large (66%) soluble fraction. Significant differences in the OTU estimates (Chao1, Ace, and Rarefaction) were detected between fractions of both diets, with bermudagrass hay supporting greater diversity than wheat forage. Sequences were compared with a 16S database using BLASTn and assigned sequences to respective genera and genera-like units based on the similarity value to known sequences in the database. Predominant genera were Prevotella (up to 33%) and Rikenella-like (up to 28%) genera on the bermudagrass diet and Prevotella (up to 56%) genus on the wheat diet irrespective of the fractions. Principle component analyses accounted for over 95% of variation in 16S estimated bacterial community composition in all three fractions and clearly differentiated communities associated with each diet. Overall, bermudagrass hay diets clustered more clearly than wheat diets. These data are the first to explore bacterial diversity dynamics in a common population of animals in response to contrasting grass forage diets.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6433589" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bdb08ee665f4f9455fc2f9ee8a78464d" rel="nofollow" data-download="{"attachment_id":48869670,"asset_id":6433589,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48869670/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_6433589 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6433589"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6433589, container: ".js-paper-rank-work_6433589", }); 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We hypothesized that very distinct community assemblages would develop between the fiber and liquid fractions of rumen contents in animals transitioned from bermudagrass hay diet to a grazed wheat diet. To address this hypothesis, we designed an experiment utilizing a 16S-based bTEFAP pyrosequencing technique to characterize and elucidate changes in bacterial diversity among the fiber and liquid rumen fractions and whole rumen contents of 14 (Angus × Hereford) ruminally cannulated steers sequentially fed bermudagrass hay (Cynodon dactylon; 34 days) and grazing wheat forage (28 days). Bermudagrass hay was a conserved C4 perennial grass lower in protein and higher in fiber (11% and 67%, respectively) content than grazed winter wheat (Triticum aestivum), a C3 annual grass with higher protein (20%) and a large (66%) soluble fraction. Significant differences in the OTU estimates (Chao1, Ace, and Rarefaction) were detected between fractions of both diets, with bermudagrass hay supporting greater diversity than wheat forage. Sequences were compared with a 16S database using BLASTn and assigned sequences to respective genera and genera-like units based on the similarity value to known sequences in the database. Predominant genera were Prevotella (up to 33%) and Rikenella-like (up to 28%) genera on the bermudagrass diet and Prevotella (up to 56%) genus on the wheat diet irrespective of the fractions. Principle component analyses accounted for over 95% of variation in 16S estimated bacterial community composition in all three fractions and clearly differentiated communities associated with each diet. Overall, bermudagrass hay diets clustered more clearly than wheat diets. These data are the first to explore bacterial diversity dynamics in a common population of animals in response to contrasting grass forage diets.","publication":"Microbial Ecology","publication_with_fallback":"Microbial 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pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology","nofollow":true},{"id":5069,"name":"Principal Component Analysis","url":"https://www.academia.edu/Documents/in/Principal_Component_Analysis","nofollow":true},{"id":6389,"name":"Microbial Ecology","url":"https://www.academia.edu/Documents/in/Microbial_Ecology","nofollow":true},{"id":9846,"name":"Ecology","url":"https://www.academia.edu/Documents/in/Ecology","nofollow":true},{"id":11417,"name":"Population Dynamics","url":"https://www.academia.edu/Documents/in/Population_Dynamics"},{"id":17825,"name":"Biodiversity","url":"https://www.academia.edu/Documents/in/Biodiversity"},{"id":54238,"name":"Triticum Aestivum","url":"https://www.academia.edu/Documents/in/Triticum_Aestivum"},{"id":94111,"name":"Animal Feed","url":"https://www.academia.edu/Documents/in/Animal_Feed"},{"id":197794,"name":"Plant Nutritive Value","url":"https://www.academia.edu/Documents/in/Plant_Nutritive_Value"},{"id":260829,"name":"Cattle","url":"https://www.academia.edu/Documents/in/Cattle"},{"id":332754,"name":"Bacterial Diversity","url":"https://www.academia.edu/Documents/in/Bacterial_Diversity"},{"id":387102,"name":"Environmental Conditions","url":"https://www.academia.edu/Documents/in/Environmental_Conditions"},{"id":516085,"name":"Winter Wheat","url":"https://www.academia.edu/Documents/in/Winter_Wheat"},{"id":684370,"name":"Cynodon Dactylon","url":"https://www.academia.edu/Documents/in/Cynodon_Dactylon"},{"id":991443,"name":"Triticum","url":"https://www.academia.edu/Documents/in/Triticum"},{"id":1030683,"name":"Rumen","url":"https://www.academia.edu/Documents/in/Rumen"},{"id":1643752,"name":"Perennial Grass","url":"https://www.academia.edu/Documents/in/Perennial_Grass"},{"id":2369223,"name":"Nutritive Value","url":"https://www.academia.edu/Documents/in/Nutritive_Value"},{"id":2480309,"name":"Nutrient density","url":"https://www.academia.edu/Documents/in/Nutrient_density"}],"publication_year":2010,"publication_year_with_fallback":2010,"paper_rank":null,"all_time_views":56,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782739" data-work_id="103782739" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782739/The_effect_of_3_nitrooxypropanol_a_potent_methane_inhibitor_on_ruminal_microbial_gene_expression_profiles_in_dairy_cows">The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background Enteric methane emissions from dairy cows are an environmental problem as well as a gross feed energy loss to the animal. Methane is generated in the rumen by methanogenic archaea from hydrogen (H2) + carbon dioxide and from... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782739" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background Enteric methane emissions from dairy cows are an environmental problem as well as a gross feed energy loss to the animal. Methane is generated in the rumen by methanogenic archaea from hydrogen (H2) + carbon dioxide and from H2 + methanol or methylamines. The methanogenic substrates are provided by non-methanogens during feed fermentation. Methane mitigation approaches have yielded variable results, partially due to an incomplete understanding of the contribution of hydrogenotrophic and methylotrophic archaea to methanogenesis. Research indicates that 3-nitrooxypropanol (3-NOP) reduces enteric methane formation in dairy cows by inhibiting methyl-coenzyme M reductase (MCR), the enzyme responsible for methane formation. The purpose of this study was to utilize metagenomic and metatranscriptomic approaches to investigate the effect of 3-NOP on the rumen microbiome and to determine the fate of H2 that accumulates less than expected under inhibited methanogenesis. Results The ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782739" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0dda5f61ebec12ebd9218d5fd994fd1b" rel="nofollow" data-download="{"attachment_id":103694395,"asset_id":103782739,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694395/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782739 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782739"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782739, container: ".js-paper-rank-work_103782739", }); 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$(".js-view-count[data-work-id=103782739]").text(description); $(".js-view-count-work_103782739").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_103782739").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="103782739"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">5</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="104196" rel="nofollow" href="https://www.academia.edu/Documents/in/Archaea">Archaea</a>, <script data-card-contents-for-ri="104196" type="text/json">{"id":104196,"name":"Archaea","url":"https://www.academia.edu/Documents/in/Archaea","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="109079" rel="nofollow" href="https://www.academia.edu/Documents/in/Methanogenesis">Methanogenesis</a>, <script data-card-contents-for-ri="109079" type="text/json">{"id":109079,"name":"Methanogenesis","url":"https://www.academia.edu/Documents/in/Methanogenesis","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="156347" rel="nofollow" href="https://www.academia.edu/Documents/in/Methane">Methane</a>, <script data-card-contents-for-ri="156347" type="text/json">{"id":156347,"name":"Methane","url":"https://www.academia.edu/Documents/in/Methane","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="348435" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiome">Microbiome</a><script data-card-contents-for-ri="348435" type="text/json">{"id":348435,"name":"Microbiome","url":"https://www.academia.edu/Documents/in/Microbiome","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=103782739]'), work: {"id":103782739,"title":"The effect of 3-nitrooxypropanol, a potent methane inhibitor, on ruminal microbial gene expression profiles in dairy cows","created_at":"2023-06-23T17:10:05.845-07:00","owner_id":10149035,"url":"https://www.academia.edu/103782739/The_effect_of_3_nitrooxypropanol_a_potent_methane_inhibitor_on_ruminal_microbial_gene_expression_profiles_in_dairy_cows","slug":"The_effect_of_3_nitrooxypropanol_a_potent_methane_inhibitor_on_ruminal_microbial_gene_expression_profiles_in_dairy_cows","dom_id":"work_103782739","summary":"Background Enteric methane emissions from dairy cows are an environmental problem as well as a gross feed energy loss to the animal. Methane is generated in the rumen by methanogenic archaea from hydrogen (H2) + carbon dioxide and from H2 + methanol or methylamines. The methanogenic substrates are provided by non-methanogens during feed fermentation. Methane mitigation approaches have yielded variable results, partially due to an incomplete understanding of the contribution of hydrogenotrophic and methylotrophic archaea to methanogenesis. Research indicates that 3-nitrooxypropanol (3-NOP) reduces enteric methane formation in dairy cows by inhibiting methyl-coenzyme M reductase (MCR), the enzyme responsible for methane formation. The purpose of this study was to utilize metagenomic and metatranscriptomic approaches to investigate the effect of 3-NOP on the rumen microbiome and to determine the fate of H2 that accumulates less than expected under inhibited methanogenesis. Results The ...","publication":null,"publication_with_fallback":null,"downloadable_attachments":[{"id":103694395,"asset_id":103782739,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694395/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694395/s40168-022-01341-9-libre.pdf?1687567279=\u0026response-content-disposition=attachment%3B+filename%3DThe_effect_of_3_nitrooxypropanol_a_poten.pdf\u0026Expires=1740604040\u0026Signature=A93NxEqKWRDnfE7QOPqiWXsdVmi23omojBKAcngMATQfluzVeBw3ecELgb3gdiAopox8CXRORRmA0HW3s-g1MXge7nJapvKWfvNnqAUw7Qa9R4jxFEcJWBtHvGTcds8e4rF2VM~T5M~6OWYwjf4H6AI-NmiEr7Hwybbc8y8YBxhc5~KA1ZOKW6K-FvLkYh76GSGi8chV8xx6gtu6-gB920yfx-LahYBF~2OkDYK6FjXnLGC6rWcvn8KnIkK9XPSC7WxsZlFBrtoT~YlV0wirerDNJWXdpGPBMOjH29EfK03ACfprBNO3EoMU9QGhdWaUEYmL1xCiTykDH7aDedJrYw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694395/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694395/mini_magick20230624-1-kb7l2f.png?1687565516"}],"downloadable_attachments_with_full_thumbnails":[{"id":103694395,"asset_id":103782739,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694395/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694395/s40168-022-01341-9-libre.pdf?1687567279=\u0026response-content-disposition=attachment%3B+filename%3DThe_effect_of_3_nitrooxypropanol_a_poten.pdf\u0026Expires=1740604040\u0026Signature=A93NxEqKWRDnfE7QOPqiWXsdVmi23omojBKAcngMATQfluzVeBw3ecELgb3gdiAopox8CXRORRmA0HW3s-g1MXge7nJapvKWfvNnqAUw7Qa9R4jxFEcJWBtHvGTcds8e4rF2VM~T5M~6OWYwjf4H6AI-NmiEr7Hwybbc8y8YBxhc5~KA1ZOKW6K-FvLkYh76GSGi8chV8xx6gtu6-gB920yfx-LahYBF~2OkDYK6FjXnLGC6rWcvn8KnIkK9XPSC7WxsZlFBrtoT~YlV0wirerDNJWXdpGPBMOjH29EfK03ACfprBNO3EoMU9QGhdWaUEYmL1xCiTykDH7aDedJrYw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694395/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694395/mini_magick20230624-1-kb7l2f.png?1687565516"}],"has_pdf":true,"has_fulltext":true,"page_count":21,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":104196,"name":"Archaea","url":"https://www.academia.edu/Documents/in/Archaea","nofollow":true},{"id":109079,"name":"Methanogenesis","url":"https://www.academia.edu/Documents/in/Methanogenesis","nofollow":true},{"id":156347,"name":"Methane","url":"https://www.academia.edu/Documents/in/Methane","nofollow":true},{"id":348435,"name":"Microbiome","url":"https://www.academia.edu/Documents/in/Microbiome","nofollow":true},{"id":1030683,"name":"Rumen","url":"https://www.academia.edu/Documents/in/Rumen"}],"publication_year":null,"publication_year_with_fallback":null,"paper_rank":null,"all_time_views":0,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782749" data-work_id="103782749" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782749/Associative_patterns_among_anaerobic_fungi_methanogenic_archaea_and_bacterial_communities_in_response_to_changes_in_diet_and_age_in_the_rumen_of_dairy_cows">Associative patterns among anaerobic fungi, methanogenic archaea, and bacterial communities in response to changes in diet and age in the rumen of dairy cows</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The rumen microbiome represents a complex microbial genetic web where bacteria, anaerobic rumen fungi (ARF), protozoa and archaea work in harmony contributing to the health and productivity of ruminants. We hypothesized that the rumen... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782749" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The rumen microbiome represents a complex microbial genetic web where bacteria, anaerobic rumen fungi (ARF), protozoa and archaea work in harmony contributing to the health and productivity of ruminants. We hypothesized that the rumen microbiome shifts as the dairy cow advances in lactations and these microbial changes may contribute to differences in productivity between primiparous (first lactation) and multiparous (≥second lactation) cows. To this end, we investigated shifts in the ruminal ARF and methanogenic communities in both primiparous (n = 5) and multiparous (n = 5) cows as they transitioned from a high forage to a high grain diet upon initiation of lactation. A total of 20 rumen samples were extracted for genomic DNA, amplified using archaeal and fungal specific primers, sequenced on a 454 platform and analyzed using QIIME. Community comparisons (Bray-Curtis index) revealed the effect of diet (P &lt; 0.01) on ARF composition, while archaeal communities differed between pr...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782749" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="dcbf5d9b9262beb8beef6761f70b010b" rel="nofollow" data-download="{"attachment_id":103694399,"asset_id":103782749,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694399/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782749 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782749"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782749, container: ".js-paper-rank-work_103782749", }); 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We hypothesized that the rumen microbiome shifts as the dairy cow advances in lactations and these microbial changes may contribute to differences in productivity between primiparous (first lactation) and multiparous (≥second lactation) cows. To this end, we investigated shifts in the ruminal ARF and methanogenic communities in both primiparous (n = 5) and multiparous (n = 5) cows as they transitioned from a high forage to a high grain diet upon initiation of lactation. A total of 20 rumen samples were extracted for genomic DNA, amplified using archaeal and fungal specific primers, sequenced on a 454 platform and analyzed using QIIME. Community comparisons (Bray-Curtis index) revealed the effect of diet (P \u0026lt; 0.01) on ARF composition, while archaeal communities differed between pr...","publication":"Frontiers in microbiology","publication_with_fallback":"Frontiers in microbiology","downloadable_attachments":[{"id":103694399,"asset_id":103782749,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694399/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694399/1a3b7640e4c8f8936bb3ac74d9907d2a6573-libre.pdf?1687567267=\u0026response-content-disposition=attachment%3B+filename%3DAssociative_patterns_among_anaerobic_fun.pdf\u0026Expires=1740553286\u0026Signature=QhinUVq850yTh2FTMoXpNcVrgKxQ9BY46LRjOFjT3SgD7nS6vKOY5e062XO4lWT2KENN3YRIiQsMvvnCrViW6i7gqzxhyu9q7DOl-0Rf7tgiCz6dRLR3Dc92ES7NmgP1C866ftfcQXkVPJdQf0OilXKqytwIjjCUU~uAF-fWu21tjV25O3Z~nPUUjoRxY5sTXRjGJFUmMCKBuXnFzwFZjCHB5uVtMcrNCvSVgzCzTuKe1i6n~QXtOyQ8ovRdK~B1zYeXpRGLdVweSQ436BPYpzgNMB9uIihpgapA7ba1J0ik75by85ZArjUn6LNp5ZbwvNM5rHUgaIM15LEHyjC5Vg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694399/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694399/mini_magick20230624-1-4res74.png?1687565522"}],"downloadable_attachments_with_full_thumbnails":[{"id":103694399,"asset_id":103782749,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694399/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694399/1a3b7640e4c8f8936bb3ac74d9907d2a6573-libre.pdf?1687567267=\u0026response-content-disposition=attachment%3B+filename%3DAssociative_patterns_among_anaerobic_fun.pdf\u0026Expires=1740553286\u0026Signature=QhinUVq850yTh2FTMoXpNcVrgKxQ9BY46LRjOFjT3SgD7nS6vKOY5e062XO4lWT2KENN3YRIiQsMvvnCrViW6i7gqzxhyu9q7DOl-0Rf7tgiCz6dRLR3Dc92ES7NmgP1C866ftfcQXkVPJdQf0OilXKqytwIjjCUU~uAF-fWu21tjV25O3Z~nPUUjoRxY5sTXRjGJFUmMCKBuXnFzwFZjCHB5uVtMcrNCvSVgzCzTuKe1i6n~QXtOyQ8ovRdK~B1zYeXpRGLdVweSQ436BPYpzgNMB9uIihpgapA7ba1J0ik75by85ZArjUn6LNp5ZbwvNM5rHUgaIM15LEHyjC5Vg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694399/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694399/mini_magick20230624-1-4res74.png?1687565522"}],"has_pdf":true,"has_fulltext":true,"page_count":10,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology","nofollow":true},{"id":19826,"name":"Lactation","url":"https://www.academia.edu/Documents/in/Lactation","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine","nofollow":true},{"id":104196,"name":"Archaea","url":"https://www.academia.edu/Documents/in/Archaea","nofollow":true},{"id":348435,"name":"Microbiome","url":"https://www.academia.edu/Documents/in/Microbiome"},{"id":1030683,"name":"Rumen","url":"https://www.academia.edu/Documents/in/Rumen"}],"publication_year":2015,"publication_year_with_fallback":2015,"paper_rank":null,"all_time_views":0,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782750" data-work_id="103782750" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782750/A_comparison_of_rumen_microbial_profiles_in_dairy_cows_as_retrieved_by_454_Roche_and_Ion_Torrent_PGM_sequencing_platforms">A comparison of rumen microbial profiles in dairy cows as retrieved by 454 Roche and Ion Torrent (PGM) sequencing platforms</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Next generation sequencing (NGS) technology is a widely accepted tool used by microbial ecologists to explore complex microbial communities in different ecosystems. As new NGS platforms continue to become available, it becomes imperative... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782750" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Next generation sequencing (NGS) technology is a widely accepted tool used by microbial ecologists to explore complex microbial communities in different ecosystems. As new NGS platforms continue to become available, it becomes imperative to compare data obtained from different platforms and analyze their effect on microbial community structure. In the present study, we compared sequencing data from both the 454 and Ion Torrent (PGM) platforms on the same DNA samples obtained from the rumen of dairy cows during their transition period. Despite the substantial difference in the number of reads, error rate and length of reads among both platforms, we identified similar community composition between the two data sets. Procrustes analysis revealed similar correlations (M (2) = 0.319; P = 0.001) in the microbial community composition between the two platforms. Both platforms revealed the abundance of the same bacterial phyla which were Bacteroidetes and Firmicutes; however, PGM recovered ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782750" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5e64208d60588ed6fdd7d43fff7ea8ce" rel="nofollow" data-download="{"attachment_id":103694398,"asset_id":103782750,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694398/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782750 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782750"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782750, container: ".js-paper-rank-work_103782750", }); 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As new NGS platforms continue to become available, it becomes imperative to compare data obtained from different platforms and analyze their effect on microbial community structure. In the present study, we compared sequencing data from both the 454 and Ion Torrent (PGM) platforms on the same DNA samples obtained from the rumen of dairy cows during their transition period. Despite the substantial difference in the number of reads, error rate and length of reads among both platforms, we identified similar community composition between the two data sets. Procrustes analysis revealed similar correlations (M (2) = 0.319; P = 0.001) in the microbial community composition between the two platforms. Both platforms revealed the abundance of the same bacterial phyla which were Bacteroidetes and Firmicutes; however, PGM recovered ...","publication":"PeerJ","publication_with_fallback":"PeerJ","downloadable_attachments":[{"id":103694398,"asset_id":103782750,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694398/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694398/1599-libre.pdf?1687567266=\u0026response-content-disposition=attachment%3B+filename%3DA_comparison_of_rumen_microbial_profiles.pdf\u0026Expires=1740553286\u0026Signature=OCQIJbMNN6NBHeCc4hOemF2YtQSEAaamH6~F3kOzhF5pxECQLnTBary0gCP1nj1dLSx-EQTMR-VOiMfOjSNsO8HT9QULq3tu19QIfF9mB-r3D-ETbeilN8hOjFvqfUBSSzPprtsMJGywIzP~oRDO-LmrcjQVhO9PcxlhOVcGW2p1vjDctW0QKPWWjNhlbD1kwf9pyZVGnJAfLWW8NsQ1SpL2H07ovmr0EoDLwI9U8-SXiK-lXwJhVgdna1xWXmZDxQ~6gOg0LHF8Tt~jZpuyIvlcGreLuiSkFbGt0Kij~0gMUVqdRZKD5~RvUysYlxOwgklj841RRH1OdkVq1i-paw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694398/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694398/mini_magick20230624-1-p1mrm.png?1687565522"}],"downloadable_attachments_with_full_thumbnails":[{"id":103694398,"asset_id":103782750,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694398/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694398/1599-libre.pdf?1687567266=\u0026response-content-disposition=attachment%3B+filename%3DA_comparison_of_rumen_microbial_profiles.pdf\u0026Expires=1740553286\u0026Signature=OCQIJbMNN6NBHeCc4hOemF2YtQSEAaamH6~F3kOzhF5pxECQLnTBary0gCP1nj1dLSx-EQTMR-VOiMfOjSNsO8HT9QULq3tu19QIfF9mB-r3D-ETbeilN8hOjFvqfUBSSzPprtsMJGywIzP~oRDO-LmrcjQVhO9PcxlhOVcGW2p1vjDctW0QKPWWjNhlbD1kwf9pyZVGnJAfLWW8NsQ1SpL2H07ovmr0EoDLwI9U8-SXiK-lXwJhVgdna1xWXmZDxQ~6gOg0LHF8Tt~jZpuyIvlcGreLuiSkFbGt0Kij~0gMUVqdRZKD5~RvUysYlxOwgklj841RRH1OdkVq1i-paw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694398/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694398/mini_magick20230624-1-p1mrm.png?1687565522"}],"has_pdf":true,"has_fulltext":true,"page_count":15,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology","nofollow":true},{"id":21948,"name":"Metagenomics","url":"https://www.academia.edu/Documents/in/Metagenomics","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine","nofollow":true},{"id":34492,"name":"Microbial Population Biology","url":"https://www.academia.edu/Documents/in/Microbial_Population_Biology","nofollow":true},{"id":55409,"name":"Dna Sequencing","url":"https://www.academia.edu/Documents/in/Dna_Sequencing"},{"id":751684,"name":"Firmicutes","url":"https://www.academia.edu/Documents/in/Firmicutes"},{"id":4106666,"name":"bacteroidetes","url":"https://www.academia.edu/Documents/in/bacteroidetes"}],"publication_year":2016,"publication_year_with_fallback":2016,"paper_rank":null,"all_time_views":0,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782752" data-work_id="103782752" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782752/Metagenomic_Evidence_of_the_Prevalence_and_Distribution_Patterns_of_Antimicrobial_Resistance_Genes_in_Dairy_Agroecosystems">Metagenomic Evidence of the Prevalence and Distribution Patterns of Antimicrobial Resistance Genes in Dairy Agroecosystems</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Antimicrobial resistance (AR) is a global problem with serious implications for public health. AR genes are frequently detected on animal farms, but little is known about their origin and distribution patterns. We hypothesized that AR... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782752" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Antimicrobial resistance (AR) is a global problem with serious implications for public health. AR genes are frequently detected on animal farms, but little is known about their origin and distribution patterns. We hypothesized that AR genes can transfer from animal feces to the environment through manure, and to this end, we characterized and compared the resistomes (collections of AR genes) of animal feces, manure, and soil samples collected from five dairy farms using a metagenomics approach. Resistomes constituted only up to 1% of the total gene content, but were variable by sector and also farm. Broadly, the identified AR genes were associated with 18 antibiotic resistances classes across all samples; however, the most abundant genes were classified under multidrug transporters (44.75%), followed by resistance to vancomycin (12.48%), tetracycline (10.52%), bacitracin (10.43%), beta-lactam resistance (7.12%), and MLS efflux pump (6.86%) antimicrobials. The AR gene profiles were v...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782752" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b26d6a524e12652a3ba86e11a1f50849" rel="nofollow" data-download="{"attachment_id":103694421,"asset_id":103782752,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694421/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782752 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782752"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782752, container: ".js-paper-rank-work_103782752", }); 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AR genes are frequently detected on animal farms, but little is known about their origin and distribution patterns. We hypothesized that AR genes can transfer from animal feces to the environment through manure, and to this end, we characterized and compared the resistomes (collections of AR genes) of animal feces, manure, and soil samples collected from five dairy farms using a metagenomics approach. Resistomes constituted only up to 1% of the total gene content, but were variable by sector and also farm. Broadly, the identified AR genes were associated with 18 antibiotic resistances classes across all samples; however, the most abundant genes were classified under multidrug transporters (44.75%), followed by resistance to vancomycin (12.48%), tetracycline (10.52%), bacitracin (10.43%), beta-lactam resistance (7.12%), and MLS efflux pump (6.86%) antimicrobials. 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Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"},{"id":4106666,"name":"bacteroidetes","url":"https://www.academia.edu/Documents/in/bacteroidetes"}],"publication_year":2016,"publication_year_with_fallback":2016,"paper_rank":null,"all_time_views":4,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782753" data-work_id="103782753" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782753/Dysbiosis_of_the_Fecal_Microbiota_in_Cattle_Infected_with_Mycobacterium_avium_subsp_paratuberculosis">Dysbiosis of the Fecal Microbiota in Cattle Infected with Mycobacterium avium subsp. paratuberculosis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Johne&#39;s disease (JD) is a chronic, intestinal infection of cattle, caused by Mycobacterium avium subsp. paratuberculosis (MAP). It results in granulomatous inflammation of the intestinal lining, leading to malabsorption, diarrhea, and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782753" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Johne&#39;s disease (JD) is a chronic, intestinal infection of cattle, caused by Mycobacterium avium subsp. paratuberculosis (MAP). It results in granulomatous inflammation of the intestinal lining, leading to malabsorption, diarrhea, and weight loss. Crohn&#39;s disease (CD), a chronic, inflammatory gastrointestinal disease of humans, has many clinical and pathologic similarities to JD. Dysbiosis of the enteric microbiota has been demonstrated in CD patients. It is speculated that this dysbiosis may contribute to the intestinal inflammation observed in those patients. The purpose of this study was to investigate the diversity patterns of fecal bacterial populations in cattle infected with MAP, compared to those of uninfected control cattle, using phylogenomic analysis. Fecal samples were selected to include samples from 20 MAP-positive cows; 25 MAP-negative herdmates; and 25 MAP-negative cows from a MAP-free herd. The genomic DNA was extracted; PCR amplified sequenced on a 454 Roch...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782753" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e6de3182457adf1bbb71369664e425d7" rel="nofollow" data-download="{"attachment_id":103694422,"asset_id":103782753,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694422/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782753 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782753"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782753, container: ".js-paper-rank-work_103782753", }); 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high and low yielding dairy cows within and between two dairy herds. Eighty five Holstein dairy cows in mid-lactation (79-179 days in milk) were selected from two... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782754" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The purpose of this study was to compare the rumen bacterial composition in high and low yielding dairy cows within and between two dairy herds. Eighty five Holstein dairy cows in mid-lactation (79-179 days in milk) were selected from two farms: Farm 12 (M305 = 12,300 kg; n = 47; 24 primiparous cows, 23 multiparous cows) and Farm 9 (M305 = 9700 kg; n = 38; 19 primiparous cows, 19 multiparous cows). Each study cow was sampled once using the stomach tube method and processed for 16S rRNA gene amplicon sequencing using the Ion Torrent (PGM) platform. Differences in bacterial communities between farms were greater (Adonis: R(2) = 0.16; p &lt; 0.001) than within farm. Five bacterial lineages, namely Prevotella (48-52%), unclassified Bacteroidales (10-12%), unclassified bacteria (5-8%), unclassified Succinivibrionaceae (1-7%) and unclassified Prevotellaceae (4-5%) were observed to differentiate the community clustering patterns among the two farms. A notable finding is the greater (p &lt;...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782754" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="bfc7028c2ffe83e241814e0eae2d7db1" rel="nofollow" data-download="{"attachment_id":103694423,"asset_id":103782754,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694423/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782754 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782754"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782754, container: ".js-paper-rank-work_103782754", }); 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Eighty five Holstein dairy cows in mid-lactation (79-179 days in milk) were selected from two farms: Farm 12 (M305 = 12,300 kg; n = 47; 24 primiparous cows, 23 multiparous cows) and Farm 9 (M305 = 9700 kg; n = 38; 19 primiparous cows, 19 multiparous cows). Each study cow was sampled once using the stomach tube method and processed for 16S rRNA gene amplicon sequencing using the Ion Torrent (PGM) platform. Differences in bacterial communities between farms were greater (Adonis: R(2) = 0.16; p \u0026lt; 0.001) than within farm. Five bacterial lineages, namely Prevotella (48-52%), unclassified Bacteroidales (10-12%), unclassified bacteria (5-8%), unclassified Succinivibrionaceae (1-7%) and unclassified Prevotellaceae (4-5%) were observed to differentiate the community clustering patterns among the two farms. 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Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"}],"publication_year":2017,"publication_year_with_fallback":2017,"paper_rank":null,"all_time_views":0,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782755" data-work_id="103782755" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782755/Metagenomic_Analysis_of_the_Rumen_Microbiome_of_Steers_with_Wheat_Induced_Frothy_Bloat">Metagenomic Analysis of the Rumen Microbiome of Steers with Wheat-Induced Frothy Bloat</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Frothy bloat is a serious metabolic disorder that affects stocker cattle grazing hard red winter wheat forage in the Southern Great Plains causing reduced performance, morbidity, and mortality. We hypothesize that a microbial dysbiosis... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782755" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Frothy bloat is a serious metabolic disorder that affects stocker cattle grazing hard red winter wheat forage in the Southern Great Plains causing reduced performance, morbidity, and mortality. We hypothesize that a microbial dysbiosis develops in the rumen microbiome of stocker cattle when grazing on high quality winter wheat pasture that predisposes them to frothy bloat risk. In this study, rumen contents were harvested from six cannulated steers grazing hard red winter wheat (three with bloat score "2" and three with bloat score "0"), extracted for genomic DNA and subjected to 16S rDNA and shotgun sequencing on 454/Roche platform. Approximately 1.5 million reads were sequenced, assembled and assigned for phylogenetic and functional annotations. Bacteria predominated up to 84% of the sequences while archaea contributed to nearly 5% of the sequences. The abundance of archaea was higher in bloated animals (P < 0.05) and dominated by Methanobrevibacter. Predominant bacterial phyla were Firmicutes (65%), Actinobacteria (13%), Bacteroidetes (10%), and Proteobacteria (6%) across all samples. Genera from Firmicutes such as Clostridium, Eubacterium, and Butyrivibrio increased (P < 0.05) while Prevotella from Bacteroidetes decreased in bloated samples. Co-occurrence analysis revealed syntrophic associations between bacteria and archaea in non-bloated samples, however; such interactions faded in bloated samples. Functional annotations of assembled reads to Subsystems database revealed the abundance of several metabolic pathways, with carbohydrate and protein metabolism well represented. Assignment of contigs to CaZy database revealed a greater diversity of Glycosyl Hydrolases dominated by oligosaccharide breaking enzymes (>70%) in non-bloated samples. However, the abundance and diversity of CaZymes were greatly reduced in bloated samples indicating the disruption of carbohydrate metabolism. We conclude that mild to moderate frothy bloat results from tradeoffs both within and between microbial domains due to greater competition for substrates that are of limited availability as a result of biofilm formation.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782755" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="050e4c527e2d0010a3fa47cf3410f293" rel="nofollow" data-download="{"attachment_id":103694401,"asset_id":103782755,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694401/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782755 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782755"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782755, container: ".js-paper-rank-work_103782755", }); 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We hypothesize that a microbial dysbiosis develops in the rumen microbiome of stocker cattle when grazing on high quality winter wheat pasture that predisposes them to frothy bloat risk. In this study, rumen contents were harvested from six cannulated steers grazing hard red winter wheat (three with bloat score \"2\" and three with bloat score \"0\"), extracted for genomic DNA and subjected to 16S rDNA and shotgun sequencing on 454/Roche platform. Approximately 1.5 million reads were sequenced, assembled and assigned for phylogenetic and functional annotations. Bacteria predominated up to 84% of the sequences while archaea contributed to nearly 5% of the sequences. The abundance of archaea was higher in bloated animals (P \u003c 0.05) and dominated by Methanobrevibacter. Predominant bacterial phyla were Firmicutes (65%), Actinobacteria (13%), Bacteroidetes (10%), and Proteobacteria (6%) across all samples. Genera from Firmicutes such as Clostridium, Eubacterium, and Butyrivibrio increased (P \u003c 0.05) while Prevotella from Bacteroidetes decreased in bloated samples. Co-occurrence analysis revealed syntrophic associations between bacteria and archaea in non-bloated samples, however; such interactions faded in bloated samples. Functional annotations of assembled reads to Subsystems database revealed the abundance of several metabolic pathways, with carbohydrate and protein metabolism well represented. Assignment of contigs to CaZy database revealed a greater diversity of Glycosyl Hydrolases dominated by oligosaccharide breaking enzymes (\u003e70%) in non-bloated samples. However, the abundance and diversity of CaZymes were greatly reduced in bloated samples indicating the disruption of carbohydrate metabolism. We conclude that mild to moderate frothy bloat results from tradeoffs both within and between microbial domains due to greater competition for substrates that are of limited availability as a result of biofilm formation.","publication":"Frontiers in Microbiology","publication_with_fallback":"Frontiers in Microbiology","downloadable_attachments":[{"id":103694401,"asset_id":103782755,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694401/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694401/85808e5a76028b9948327683a9741ace31ae-libre.pdf?1687567266=\u0026response-content-disposition=attachment%3B+filename%3DMetagenomic_Analysis_of_the_Rumen_Microb.pdf\u0026Expires=1740604040\u0026Signature=UftmLRyyCCcGT12sPnPukbCFK4CAM0j~zKSUkWO1NfPj0Xoex8GlmzabKjtb8k4RhsfOLL34A4f0g00chSuiqrGzSe8B0694bRo9Usn9fO9GmD5lPRLFoaaT0v4e-WMTsjcBPoCkwErg1Q-o5pbIgWGkfYiTqNA4npMj9nOCihbnoI2VXmDZLZq-mUvOmjWgHgY5WRasGfDE8hClIlPgxtRZHUDZrDZpUcyUWmG8rcEcoPdmb2Ncb-FVcx5TiaSGYft2lA0hNlyt-es2TVurQ8i5labHKjYygaPwjy6PIVGY75RiBrSkvCs8MNKywok08R70bBYAQcoei121vlCrZg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694401/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694401/mini_magick20230624-1-nuv475.png?1687565520"}],"downloadable_attachments_with_full_thumbnails":[{"id":103694401,"asset_id":103782755,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694401/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694401/85808e5a76028b9948327683a9741ace31ae-libre.pdf?1687567266=\u0026response-content-disposition=attachment%3B+filename%3DMetagenomic_Analysis_of_the_Rumen_Microb.pdf\u0026Expires=1740604040\u0026Signature=UftmLRyyCCcGT12sPnPukbCFK4CAM0j~zKSUkWO1NfPj0Xoex8GlmzabKjtb8k4RhsfOLL34A4f0g00chSuiqrGzSe8B0694bRo9Usn9fO9GmD5lPRLFoaaT0v4e-WMTsjcBPoCkwErg1Q-o5pbIgWGkfYiTqNA4npMj9nOCihbnoI2VXmDZLZq-mUvOmjWgHgY5WRasGfDE8hClIlPgxtRZHUDZrDZpUcyUWmG8rcEcoPdmb2Ncb-FVcx5TiaSGYft2lA0hNlyt-es2TVurQ8i5labHKjYygaPwjy6PIVGY75RiBrSkvCs8MNKywok08R70bBYAQcoei121vlCrZg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694401/download_file?st=MTc0MDYwMDQ0MCw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694401/mini_magick20230624-1-nuv475.png?1687565520"}],"has_pdf":true,"has_fulltext":true,"page_count":11,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology","nofollow":true},{"id":21948,"name":"Metagenomics","url":"https://www.academia.edu/Documents/in/Metagenomics","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine","nofollow":true},{"id":55168,"name":"Actinobacteria","url":"https://www.academia.edu/Documents/in/Actinobacteria","nofollow":true},{"id":104196,"name":"Archaea","url":"https://www.academia.edu/Documents/in/Archaea"},{"id":751684,"name":"Firmicutes","url":"https://www.academia.edu/Documents/in/Firmicutes"},{"id":1030683,"name":"Rumen","url":"https://www.academia.edu/Documents/in/Rumen"},{"id":2235564,"name":"Proteobacteria","url":"https://www.academia.edu/Documents/in/Proteobacteria"},{"id":4106666,"name":"bacteroidetes","url":"https://www.academia.edu/Documents/in/bacteroidetes"}],"publication_year":2016,"publication_year_with_fallback":2016,"paper_rank":null,"all_time_views":2,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782756" data-work_id="103782756" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782756/Alterations_in_ruminal_bacterial_populations_at_induction_and_recovery_from_diet_induced_milk_fat_depression_in_dairy_cows">Alterations in ruminal bacterial populations at induction and recovery from diet-induced milk fat depression in dairy cows</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Ten ruminally cannulated Holstein cows were used in a crossover design that investigated changes in ruminal bacterial populations in response to induction and recovery from diet-induced milk fat depression (MFD). Further, the effect on... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782756" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Ten ruminally cannulated Holstein cows were used in a crossover design that investigated changes in ruminal bacterial populations in response to induction and recovery from diet-induced milk fat depression (MFD). Further, the effect on the ruminal microbiota of the cows with diet-induced milk fat depression inoculated with rumen contents from non-milk fat-depressed donor cows was evaluated. Milk fat depression was induced during the first 10 d of each period by feeding a low-fiber, high-starch, and high-polyunsaturated fatty acid diet (26.1% neutral detergent fiber, 28.1% starch, 5.8% total fatty acids, and 1.9% C18:2), resulting in a 30% decrease in milk fat yield. Induction was followed by a recovery phase, where all cows were switched to a high-fiber, low-starch, and low-polyunsaturated fatty acid diet (31.8% neutral detergent fiber, 23% starch, 4.2% total fatty acids, and 1.2% C18:2) and were allocated to (1) control (no inoculation) or (2) ruminal inoculation with donor cow digesta (8 kg/d for 6 d). Ruminal samples were collected at the end of induction (d 10) and during recovery (d 13, 16, and 28), separated to solid and liquid fractions, extracted for DNA, PCR-amplified for the V1-V2 region of the 16S rRNA gene, and analyzed for bacterial diversity. Results indicated that bacterial communities were different between fractions. In each fraction, differences were significant between the induction (d 10) and recovery (d 13, 16, and 28) periods; however, differences were less apparent with time during the recovery period. The MFD (d 10) was typified by a reduction in the relative sequence abundance of Bacteroidetes and an increase in the relative sequence abundance of Firmicutes and Actinobacteria across both fractions. At the genus level, relative sequence abundance of unclassified Lachnospi-raceae, Butyrivibrio, Bulleidia, and Coriobacteriaceae were higher on d 10 and were positively correlated with trans-10,cis-12 CLA and the trans-10 isomer, suggesting their potential role in altered biohydrogenation reactions. A switch to the recovery diet resulted in a sharp increase in the Bacteroidetes lineages and a decrease in Firmicutes members on d 13; however, this shift appears to stabilize by d 28, indicating the restoration process for ruminal bacteria from an altered state is gradual and complex. Inoculation of 10% of rumen contents from non-MFD donor cows to MFD cows revealed this procedure had transient effects on only a few bacterial populations, and such effects disappeared after d 16 following cessation of inoculation. It can be concluded that alterations in milk FA profiles at induction are preceded by microbial alterations in the rumen driven by dietary changes.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782756" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="daca2eac4b33f4868caa72240562bb5b" rel="nofollow" data-download="{"attachment_id":103694400,"asset_id":103782756,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694400/download_file?st=MTc0MDYwMDQ0MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782756 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782756"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782756, container: ".js-paper-rank-work_103782756", }); 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$(".js-view-count[data-work-id=103782756]").text(description); $(".js-view-count-work_103782756").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_103782756").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="103782756"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1040" rel="nofollow" href="https://www.academia.edu/Documents/in/Food_Science">Food Science</a>, <script data-card-contents-for-ri="1040" type="text/json">{"id":1040,"name":"Food Science","url":"https://www.academia.edu/Documents/in/Food_Science","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="4630" rel="nofollow" href="https://www.academia.edu/Documents/in/Dairy_Science">Dairy Science</a>, <script data-card-contents-for-ri="4630" type="text/json">{"id":4630,"name":"Dairy Science","url":"https://www.academia.edu/Documents/in/Dairy_Science","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="7710" rel="nofollow" href="https://www.academia.edu/Documents/in/Biology">Biology</a>, <script data-card-contents-for-ri="7710" type="text/json">{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="16062" rel="nofollow" href="https://www.academia.edu/Documents/in/Starch">Starch</a><script data-card-contents-for-ri="16062" type="text/json">{"id":16062,"name":"Starch","url":"https://www.academia.edu/Documents/in/Starch","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=103782756]'), work: {"id":103782756,"title":"Alterations in ruminal bacterial populations at induction and recovery from diet-induced milk fat depression in dairy cows","created_at":"2023-06-23T17:10:19.761-07:00","owner_id":10149035,"url":"https://www.academia.edu/103782756/Alterations_in_ruminal_bacterial_populations_at_induction_and_recovery_from_diet_induced_milk_fat_depression_in_dairy_cows","slug":"Alterations_in_ruminal_bacterial_populations_at_induction_and_recovery_from_diet_induced_milk_fat_depression_in_dairy_cows","dom_id":"work_103782756","summary":"Ten ruminally cannulated Holstein cows were used in a crossover design that investigated changes in ruminal bacterial populations in response to induction and recovery from diet-induced milk fat depression (MFD). Further, the effect on the ruminal microbiota of the cows with diet-induced milk fat depression inoculated with rumen contents from non-milk fat-depressed donor cows was evaluated. Milk fat depression was induced during the first 10 d of each period by feeding a low-fiber, high-starch, and high-polyunsaturated fatty acid diet (26.1% neutral detergent fiber, 28.1% starch, 5.8% total fatty acids, and 1.9% C18:2), resulting in a 30% decrease in milk fat yield. Induction was followed by a recovery phase, where all cows were switched to a high-fiber, low-starch, and low-polyunsaturated fatty acid diet (31.8% neutral detergent fiber, 23% starch, 4.2% total fatty acids, and 1.2% C18:2) and were allocated to (1) control (no inoculation) or (2) ruminal inoculation with donor cow digesta (8 kg/d for 6 d). Ruminal samples were collected at the end of induction (d 10) and during recovery (d 13, 16, and 28), separated to solid and liquid fractions, extracted for DNA, PCR-amplified for the V1-V2 region of the 16S rRNA gene, and analyzed for bacterial diversity. Results indicated that bacterial communities were different between fractions. In each fraction, differences were significant between the induction (d 10) and recovery (d 13, 16, and 28) periods; however, differences were less apparent with time during the recovery period. The MFD (d 10) was typified by a reduction in the relative sequence abundance of Bacteroidetes and an increase in the relative sequence abundance of Firmicutes and Actinobacteria across both fractions. At the genus level, relative sequence abundance of unclassified Lachnospi-raceae, Butyrivibrio, Bulleidia, and Coriobacteriaceae were higher on d 10 and were positively correlated with trans-10,cis-12 CLA and the trans-10 isomer, suggesting their potential role in altered biohydrogenation reactions. A switch to the recovery diet resulted in a sharp increase in the Bacteroidetes lineages and a decrease in Firmicutes members on d 13; however, this shift appears to stabilize by d 28, indicating the restoration process for ruminal bacteria from an altered state is gradual and complex. Inoculation of 10% of rumen contents from non-MFD donor cows to MFD cows revealed this procedure had transient effects on only a few bacterial populations, and such effects disappeared after d 16 following cessation of inoculation. It can be concluded that alterations in milk FA profiles at induction are preceded by microbial alterations in the rumen driven by dietary changes.","publication":"Journal of Dairy Science","publication_with_fallback":"Journal of Dairy Science","downloadable_attachments":[{"id":103694400,"asset_id":103782756,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694400/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694400/pdf-libre.pdf?1687567270=\u0026response-content-disposition=attachment%3B+filename%3DAlterations_in_ruminal_bacterial_populat.pdf\u0026Expires=1740604040\u0026Signature=g332f3AInRvDExxg4IXdNQSfJaEOIkgsEJIhDM-7zYTJlPDXeM8YbEWPQtRO2AxZf0iudDpAwq~JeNRzIjBtcwWmuCxURmHY4Ai3ZKGWj0wxWPd~N4xFOAHSgmlpGxwuZGPHAagoHjIQGSaq36hLMdZ~6wCVaR3OI8SP~qcfbA492yRox1l~ZGUrAQUea7YWy~z0tb1pqt~Tvljmgslea0xgK4I5yYDhy4AHp1G-plNK0Lho4ToD30GxEJGef7OG0Jb45hIkXC1q31xTAx1XMZGpsdyqIjrCd2lcbpv7uyuVvPAp~MX6BSVLqFxbFPPL-hCscjivqpO5QZ9rHC~raw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694400/download_file?st=MTc0MDYwMDQ0MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694400/mini_magick20230624-1-4h5myd.png?1687565527"}],"downloadable_attachments_with_full_thumbnails":[{"id":103694400,"asset_id":103782756,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/103694400/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/103694400/pdf-libre.pdf?1687567270=\u0026response-content-disposition=attachment%3B+filename%3DAlterations_in_ruminal_bacterial_populat.pdf\u0026Expires=1740604041\u0026Signature=JxHF3w8pIDi4ukarUqN8LQRVMY-MfXLKII53WZ8hOTTHDSqWEqJvRH2jDiixLzLK2kFzE1P40TJrbbp0mSZK~BCqpx-BIbqGdUL2vlRavkb2SFTucUGLU0DcnJOewuohuRgvXDEUql9EMg4uf6Ro9lPn2SzFahJqIMm4-AHYnzT-Tx8ultWxa69vbZORcTY8VP~dy9hqWFpb71wKR2OIWqUIK3Pc4f7Wkr1xp2eLhJgrgq0LfRk7jcgyJ-UDYk~YNvTV7UYJk~A~Gfo0J~aVe2CmmUPmn3JS2hqGMtvBGIyhOg-txXeYa1vgfLfT3rWorc1JJOB7OEcMfmzAmdayLQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/103694400/download_file?st=MTc0MDYwMDQ0MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/103694400/mini_magick20230624-1-4h5myd.png?1687565527"}],"has_pdf":true,"has_fulltext":true,"page_count":15,"ordered_authors":[{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}],"research_interests":[{"id":1040,"name":"Food Science","url":"https://www.academia.edu/Documents/in/Food_Science","nofollow":true},{"id":4630,"name":"Dairy Science","url":"https://www.academia.edu/Documents/in/Dairy_Science","nofollow":true},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology","nofollow":true},{"id":16062,"name":"Starch","url":"https://www.academia.edu/Documents/in/Starch","nofollow":true},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":29980,"name":"Animal Production","url":"https://www.academia.edu/Documents/in/Animal_Production"},{"id":486713,"name":"Fatty Acid","url":"https://www.academia.edu/Documents/in/Fatty_Acid"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences"},{"id":1030683,"name":"Rumen","url":"https://www.academia.edu/Documents/in/Rumen"},{"id":3795182,"name":"Neutral Detergent Fiber","url":"https://www.academia.edu/Documents/in/Neutral_Detergent_Fiber"}],"publication_year":2018,"publication_year_with_fallback":2018,"paper_rank":null,"all_time_views":1,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_103782757" data-work_id="103782757" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/103782757/P067_Investigation_of_the_Presence_of_Mycobacterium_Avium_Ssp_Paratuberculosis_in_Children_with_Crohn_Disease_Using_Quantitative_Dna_Sequence_Based_Approaches">P067 Investigation of the Presence of Mycobacterium Avium Ssp. 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href="https://www.academia.edu/103782758/Solar_radiation_affects_bloat_potential_of_wheat_forage">Solar radiation affects bloat potential of wheat forage</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Frothy bloat is a serious digestive disorder in cattle (&lt;em&gt;Bos taurus&lt;/em&gt; L.) grazing winter wheat (&lt;em&gt;Triticum aestivum&lt;/em&gt; L.) pastures in the Southern Great Plains of the USA. Wheat plant metabolism may be... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_103782758" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Frothy bloat is a serious digestive disorder in cattle (&lt;em&gt;Bos taurus&lt;/em&gt; L.) grazing winter wheat (&lt;em&gt;Triticum aestivum&lt;/em&gt; L.) pastures in the Southern Great Plains of the USA. Wheat plant metabolism may be one of the factors involved in bloat occurrence. In a series of experiments conducted during 2004–2007, we evaluated the effects of solar radiation intensity (ambient, 100% vs. reduced, 25%), a short-time (24 h vs. 48 h) exposure to solar radiation, and forage allowance (high, 18 kg vs. low, 6 kg DM/100 kg body weight) on seasonal concentration of phenolic compounds and foam strength (a measure of bloat potential) of wheat forage ‘Cutter’. Reduced solar radiation decreased total phenolic concentration and increased foam strength when compared to ambient solar radiation. Forage allowance interacted with solar radiation and short-term exposure treatments in determining phenolic concentrations; however, the effects were inconsistent during and among gro...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/103782758" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="49301225ba1472a7a90010872373d91d" rel="nofollow" data-download="{"attachment_id":103694376,"asset_id":103782758,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/103694376/download_file?st=MTc0MDYwMDQ0MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10149035" href="https://upenn.academia.edu/Diptipitta">Dipti pitta</a><script data-card-contents-for-user="10149035" type="text/json">{"id":10149035,"first_name":"Dipti","last_name":"pitta","domain_name":"upenn","page_name":"Diptipitta","display_name":"Dipti pitta","profile_url":"https://upenn.academia.edu/Diptipitta","photo":"https://0.academia-photos.com/10149035/84267357/72898369/s65_dipti.pitta.png"}</script></span></span></li><li class="js-paper-rank-work_103782758 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="103782758"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 103782758, container: ".js-paper-rank-work_103782758", }); 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L.) grazing winter wheat (\u0026lt;em\u0026gt;Triticum aestivum\u0026lt;/em\u0026gt; L.) pastures in the Southern Great Plains of the USA. Wheat plant metabolism may be one of the factors involved in bloat occurrence. In a series of experiments conducted during 2004–2007, we evaluated the effects of solar radiation intensity (ambient, 100% vs. reduced, 25%), a short-time (24 h vs. 48 h) exposure to solar radiation, and forage allowance (high, 18 kg vs. low, 6 kg DM/100 kg body weight) on seasonal concentration of phenolic compounds and foam strength (a measure of bloat potential) of wheat forage ‘Cutter’. Reduced solar radiation decreased total phenolic concentration and increased foam strength when compared to ambient solar radiation. 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