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li > ul.mfn-megamenu-bg{box-sizing:border-box}#Top_bar .menu > li > ul.mfn-megamenu-bg{padding:20px 166px 20px 20px;background-repeat:no-repeat;background-position:right bottom}.rtl #Top_bar .menu > li > ul.mfn-megamenu-bg{padding-left:166px;padding-right:20px;background-position:left bottom}#Top_bar .menu > li > ul.mfn-megamenu-bg > li{background:none}#Top_bar .menu > li > ul.mfn-megamenu-bg > li a{border:none}#Top_bar .menu > li > ul.mfn-megamenu-bg > li > ul{background:none!important;-webkit-box-shadow:0 0 0 0;-moz-box-shadow:0 0 0 0;box-shadow:0 0 0 0}.mm-vertical #Top_bar .container{position:relative}.mm-vertical #Top_bar .top_bar_left{position:static}.mm-vertical #Top_bar .menu > li ul{box-shadow:0 0 0 0 transparent!important;background-image:none}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu{padding:20px 0}.mm-vertical.header-plain #Top_bar .menu > li > ul.mfn-megamenu{width:100%!important;margin:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > 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#menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;transform:unset}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}.header-simple #Top_bar #menu ul li.hover > 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ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:not(.menu-toggle):after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:not(.menu-toggle):after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:not(.menu-toggle):after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span{border-width:0!important}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}.menuo-no-borders.header-plain #Top_bar a#header_cart,.menuo-no-borders.header-plain #Top_bar a#search_button,.menuo-no-borders.header-plain #Top_bar .wpml-languages,.menuo-no-borders.header-plain #Top_bar a.action_button{border-width:0}.menuo-right #Top_bar .menu_wrapper{float:right}.menuo-right.header-stack:not(.header-center) #Top_bar .menu_wrapper{margin-right:150px}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank,body.under-construction.header-rtl.header-creative.header-open{padding-left:0!important;padding-right:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background-color:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar #menu{background-color:transparent}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{margin:0;width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}.menuo-arrows #Top_bar .menu > li.submenu > a > span:after{content:unset!important}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:left;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{content:none}#Header_creative #Top_bar .top_bar_right .top_bar_right_wrapper{flex-wrap:wrap;justify-content:center}#Header_creative #Top_bar .top_bar_right .top-bar-right-icon,#Header_creative #Top_bar .top_bar_right .wpml-languages,#Header_creative #Top_bar .top_bar_right .top-bar-right-button,#Header_creative #Top_bar .top_bar_right .top-bar-right-input{min-height:30px;margin:5px}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .contact_details{width:100%;text-align:center;margin-bottom:20px}#Header_creative #Action_bar .contact_details li{padding:0}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative #Action_bar .social-menu{float:none;text-align:center}#Header_creative #Action_bar .social-menu li{border-color:rgba(0,0,0,.1)}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}.header-open #Header_creative #Action_bar{display:block}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left;width:100%}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:not(.menu-toggle):after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33;z-index:203}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+";position:static}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul.sub-menu li:not(:last-of-type) a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1240px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none;box-shadow:unset}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo,#Top_bar.is-sticky .custom-logo-link{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}.logo-sticky-width-auto #Top_bar.is-sticky #logo img.logo-sticky{width:auto}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky,.sticky-dark 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15px 30px 0 rgba(1,7,39,.13)}#mfn-gdpr .mfn-gdpr-content,#mfn-gdpr .mfn-gdpr-content h1,#mfn-gdpr .mfn-gdpr-content h2,#mfn-gdpr .mfn-gdpr-content h3,#mfn-gdpr .mfn-gdpr-content h4,#mfn-gdpr .mfn-gdpr-content h5,#mfn-gdpr .mfn-gdpr-content h6,#mfn-gdpr .mfn-gdpr-content ol,#mfn-gdpr .mfn-gdpr-content ul{color:#626262}#mfn-gdpr .mfn-gdpr-content a,#mfn-gdpr a.mfn-gdpr-readmore{color:#161922}#mfn-gdpr .mfn-gdpr-content a:hover,#mfn-gdpr a.mfn-gdpr-readmore:hover{color:#0089f7}#mfn-gdpr .mfn-gdpr-button{background-color:#006edf;color:#ffffff;border-color:transparent}#mfn-gdpr .mfn-gdpr-button:hover{background-color:#0089f7;color:#ffffff;border-color:transparent}@media only screen and ( min-width:768px ){.header-semi #Top_bar:not(.is-sticky){background-color:rgba(255,255,255,0.8)}}@media only screen and ( max-width:767px ){#Top_bar{background-color:#ffffff !important}#Action_bar{background-color:#007dc5 !important}#Action_bar .contact_details{color:#222222}#Action_bar .contact_details a{color:#0095eb}#Action_bar .contact_details a:hover{color:#007cc3}#Action_bar .social li a,#Action_bar .social-menu a{color:#bbbbbb!important}#Action_bar .social li a:hover,#Action_bar .social-menu a:hover{color:#777777!important}} form input.display-none{display:none!important} </style> <link rel='stylesheet' id='style-css' href='https://www.wyatt.com/wp-content/themes/betheme-child/style.css?ver=6.4.5' type='text/css' media='all' /> <style id='mfn-custom-inline-css' type='text/css'> @supports (-ms-ime-align: auto) { } span {font-family:"Open Sans", Arial, Tahoma, sans-serif !important;} @media only screen and (min-width: 1240px) { .header-classic #Header .top_bar_left {width:1306px;} #Top_bar .menu > li > a {padding:15px 5px;} #Top_bar.is-sticky .menu_wrapper .menu > li > a {padding:15px 5px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu{ padding-top:0px; padding-bottom:0px; width:92.5%!important; margin:0px 3%;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu .container {display:block;} /** Added 121024 **/ h1 { line-height: 44px !important; } .h2-underline {border-bottom: thick solid #ffb600; padding-bottom: 15px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu::before {content:' '; background:#fff; position:absolute; width:100vw; height:100%; margin-left: -50vw; left: 50%; } .mm-vertical #Top_bar .menu > li > .menu-overlap {z-index:1;background:rgba(0,0,0,0.4); position:absolute; width:100vw; height:100vh; margin-left: -50vw; left: 50%; cursor:hand; cursor:pointer;} .mm-vertical #Top_bar .menu > li.not-active a{color:#444444;} .mm-vertical #Top_bar .menu > li.not-active a::after {opacity:0;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title, .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover {padding:5px 0px; background:transparent; color:#444444; text-transform:unset;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title:hover span {text-decoration:none ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu ul {background:transparent; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a {color:#007dc5; } .mm-vertical #Top_bar .menu > li >ul.mfn-megamenu ul a:hover {background:transparent;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a {padding:5px 0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a:hover {text-decoration:underline;} .mm-vertical #Top_bar .menu > li > ul {padding:20px 20px 20px 2px;} .mm-vertical #Top_bar .menu > li > ul a {padding:5px; border:0px; color:#007dc5;} .mm-vertical #Top_bar .menu > li > ul a:hover {background:transparent; } .mm-vertical #Top_bar .menu > li > ul a:hover span {text-decoration:underline ;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li {padding-top:20px; padding-bottom:25px; border-right: 0px solid rgba(0, 0, 0, 0.05);} /*for parent title in sbmenu*/ .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 {padding-top:20px; line-height:30px; border-bottom:1px solid #ccc; font-weight:600;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child h3 a{color:#444444;} /*for contact submenu*/ .mm-vertical #Top_bar .menu > li#menu-item-2437 li.mfn-megamenu-cols-2 li {} /*menu button css*/ .mm-vertical #Top_bar .menu > li#menu-item-5221 a span {background:#007dc5; color:#fff; border-radius:6px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-5221 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-5221 > a, #Top_bar.is-sticky .menu > li#menu-item-5221 > a span {line-height:30px; margin-top:0px;} .mm-vertical #Top_bar li#menu-item-6152 a {color:#000 !important; padding-top:0px !important; white-space:nowrap; color: #007dc5 !important; font-weight: 400;} /*set width of menu item*/ .mm-vertical #Top_bar li#menu-item-2341 {width:343px; padding-left: 80px;} .mm-vertical #Top_bar li#menu-item-2348 {width:278px;} .mm-vertical #Top_bar li#menu-item-2355 {width:288px;} .mm-vertical #Top_bar li#menu-item-2363 {width:240px;} .mm-vertical #Top_bar li#menu-item-2369 {width:541px; padding-left:70px;} .mm-vertical #Top_bar li#menu-item-2379 {width:340px;} .mm-vertical #Top_bar li#menu-item-2384 {width:280px;} .mm-vertical #Top_bar li#menu-item-2391 {width:350px; padding-left:80px;} .mm-vertical #Top_bar li#menu-item-2395 {width:310px;} .mm-vertical #Top_bar li#menu-item-2403 {width:310px;} .mm-vertical #Top_bar li#menu-item-2408 {width:210px;} .mm-vertical #Top_bar li#menu-item-5918 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5919 {width:387px;} .mm-vertical #Top_bar li#menu-item-5920 {width:280px;} .mm-vertical #Top_bar li#menu-item-2427 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-5235 {width:487px; padding-left:115px;} .mm-vertical #Top_bar li#menu-item-5765 {width:310px;} .mm-vertical #Top_bar li#menu-item-26131 {width:387px;} .mm-vertical #Top_bar li#menu-item-15082 {width:700px; padding-left:300px;} .mm-vertical #Top_bar li#menu-item-15044 {width:397px;} } @media only screen and (max-width: 1239px) { #menu > ul > li > a, a.action_button, #overlay-menu ul li a { font-size: 15px; font-weight: 400; letter-spacing: 1px;} .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child {display:none; width:auto; padding-bottom:0px;} .mm-vertical #Top_bar #menu ul li ul li a {font-size:16px;} .mm-vertical #Top_bar #menu ul li ul li ul li a {font-size:15px;} } /* Header */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1;} .header-classic:not(.home) #Content { border-top: 0px solid #e1e1e1; } .header-classic.home .mfn-rev-slider { border-top: 1px solid #e1e1e1; } /*Action Bar*/ #Action_bar a {color:#fff;} /* Widget */ .widget-area:before { display: none; } .aside_left .widget-area { border: 0; } /* Icon box */ .icon_box.has_border { background: #fff; border: 1px solid #e1e1e1; padding: 40px 10%; } .icon_box.no_border {background: #fff; border:0px; padding: 40px 10%;} .icon_box.has_border:after {border:0px} /* Offer box */ .offer_box { background-color: #252525; padding: 20px 20px 5px; text-align: center; } .offer_box .offer_box_list { border-width: 1px 1px 0 1px; border-style: solid; border-color: #929292; font-weight: 700; font-size: 16px; letter-spacing: 2px; margin-bottom: 20px; } .offer_box .offer_box_list a { display: block; color: #fff; border-bottom: 1px solid #929292; padding: 18px 15px; word-wrap: break-word; } /* Footer */ /*#Footer { border-top: 1px solid #e1e1e1; }*/ #Footer .widget > h4 { font-size: 18px; font-weight: 300; margin-bottom: 20px; } #Footer .footer_copy { background: #465158; border-top: 0; } .before-footer {background: #f9f9f9;} #Footer .footer_copy .social { display: none; } /**/ .top-widgets-container {float:right} .top-widgets-container .widget {padding-bottom:0px; margin-bottom:0px; float:right;margin-top:0px;} .top-widgets-container .widget ul.menu{list-style:none; padding:0px;} .top-widgets-container .widget ul.menu li {float:left; padding:12px 0px;} .top-widgets-container .widget ul.menu li a{padding:0px 20px; border-right:1px solid #ccc; line-height:15px;} .top-widgets-container .widget ul.menu li:last-child a{border-right:0px;} .top-widgets-container .widget_search .icon-search-fine {font-size:24px; line-height:40px;} .top-widgets-container .widget_search input[type="text"] {width:82%; float:right; padding:5px 10px 5px 5px; border:0px; margin-top:7px; height:30px;min-height:30px;} #menu-top-navigation .cart a {padding:0px 20px; } #menu-top-navigation .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} .mobilecart {display:none; width:30px; float:right; margin-top:11px;} .mobilecart a { padding:0px; border:0px;} .mobilecart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; left:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { .top-widgets-container .widget {float:none;} .top-widgets-container .widget.widget_nav_menu {max-width:245px; margin:0px auto; clear:both;} .top-widgets-container .widget ul.menu li a {padding:0px 15px;} #menu-top-navigation .cart.menu-item-2449 {display:none;} #menu-top-navigation .cart a {padding:0px 10px;} .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .widget:after {display:none;} .top-widgets-container {float:none; margin:0px auto;} .top-widgets-container .widget_search input[type="text"] {width:80%;} .mobilecart {display:block;} .top-widgets-container .widget_search {width:254px; overflow:hidden;padding-bottom:20px; margin:0px auto;} .top-widgets-container .widget_search .searchform{width:210px; float:left;} } .icon-100 .image_wrapper img {width:150px; height:150px;} .down-heading, .heading {padding-left:17px;} @media only screen and (min-width:320px) and (max-width:767px) { .down-heading, .heading {padding:0px; text-align:center;} } .ui-tabs .ui-tabs-nav li {border-width:0px;} .page-id-2602 .ui-tabs .ui-tabs-nav li a {padding:14px 28px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li {border:0px; padding:5px 0px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {background-image:none; font-size:100%; color:#2991d6; padding:5px 10px 5px 20px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a:hover {text-decoration:underline;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a {color:#000; border-left:3px solid #2991d6; border-radius:0px !important; padding:5px 10px 5px 17px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:23%; border:0px !important;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:76.7%; border:0px !important; padding-top:5px;} @media only screen and (min-width:768px) and (max-width:1239px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {font-size:100%;} } @media only screen and (min-width:320px) and (max-width:767px) { .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav {width:100%;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-panel {width:100%; padding-top:15px;} .column_tabs .tabs_vertical.ui-tabs .ui-tabs-nav li a {-webkit-box-shadow:none;-moz-box-shadow:none;box-shadow:none;} } .ui-tabs .ui-tabs-nav li h3 {margin:0px; padding:0px;} .ui-tabs .ui-tabs-nav li h3 a { text-decoration: none; display: block; padding: 14px 30px !important; font-size: 100%; font-weight:initial; } .ui-tabs .ui-tabs-panel .icon_box {padding-bottom:2px;} .ui-tabs .ui-tabs-panel .column.one-fourth {width:25%;} .ui-tabs .ui-tabs-panel .column.one-second, .ui-tabs .ui-tabs-panel .column.one-fourth, .ui-tabs .ui-tabs-panel .column.one-third, .ui-tabs .ui-tabs-panel .column.two-third{margin-left:0px; margin-right:0px;} @media only screen and (min-width: 960px) and (max-width: 1239px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:10px 20px !important;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .ui-tabs .ui-tabs-nav li h3 a {font-size:100%; padding:5px 10px !important;} } /* Make Nav Subheadings unclickable */ .unclickable > a:hover { cursor: default;} .wp-caption-text { background: transparent; color: #075c97; font-size:90%;} .ls-wrapper h3 a {color: #075c97;} .column_sidebar_widget {float:none;} /*app notes*/ ul.clients.appnotes .client_wrapper {text-align:left; height:auto !important; line-height:auto !important; overflow:hidden;} ul.clients.appnotes li:nth-child(2n+1) {clear:both;} ul.clients.appnotes .client_wrapper img { position:relative; left:auto !important; top: auto !important; float:left; -webkit-transform: none!important; -moz-transform: none!important; transform: none!important; padding-right:20px; width:auto!important; } ul.clients.appnotes .client_wrapper h3 { line-height:20px; font-size:16px; letter-spacing:1px; padding-top:10px;} ul.clients.appnotes .client_wrapper a:hover {text-decoration:none;} @media only screen and (max-width: 640px) { ul.clients.appnotes li {width:100% !important; clear:both;} } .pager {padding-top:20px;} .pager .page-numbers {border:1px solid #ccc; padding:3px 5px;} .pager .prev.page-numbers, .pager .next.page-numbers {border:0px;} .alignleft {margin:3px 10px 10px 0px;} dl {clear:both;} .team.team_vertical img.scale-with-grid {width:100%;} .column_placeholder {margin-bottom:0px;} .icon-height img{max-height:50px;} .single .post-header {margin-left:0px; margin-right:0px;} .single .section-post-header .single-photo-wrapper.image {display:none;} .widget_mfn_menu ul li.menu-item-has-children > a::after {content: '\e868'; font-family: "mfn-icons"; right:2px; position:absolute; width:20px; height:15px; color:#ccc; } table th ul, table td ul {list-style-position:inside !important;} .sticky-white #Top_bar.is-sticky, .sticky-white #Top_bar.is-sticky #menu, #Top_bar.is-sticky {background:#fff; opacity:1;} /*Webinars latest posts*/ .parent-pageid-3663 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-3663 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-3663 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-3663 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-3663 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .parent-pageid-3663 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-3663 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-3663 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-3663 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars*/ .page-id-2653 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-2653 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-2653 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-2653 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-2653 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-2653 .Latest_news ul li.category-webinars .desc h5 a{color:#007dc5;} .page-id-2653 .Latest_news ul li .desc_footer .date {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-2653 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-2653 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-2653 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-2653 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Newsletter Archive*/ .page-id-3661 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-3661 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-3661 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-3661 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-3661 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-3661 .Latest_news ul li.category-newsletter-archive .desc h5 a{color:#007dc5;} .page-id-3661 .Latest_news ul li .desc_footer .date {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-3661 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-3661 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-3661 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-3661 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Ask the Expert*/ .page-id-5348 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-5348 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-5348 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-5348 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-5348 .Latest_news ul li .desc h5{margin-bottom:0px;} .page-id-5348 .Latest_news ul li.category-ask-the-expert .desc h5 a{color:#007dc5;} .page-id-5348 .Latest_news ul li .desc_footer {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .date {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-5348 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-5348 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-5348 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-5348 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; font-weight:normal; line-height:20px;} /*accordion title as h3*/ .accordion .question .title h3 {margin-bottom:0px; } .accordion .question.active .title h3{color:#2991d6} /*column padding*/ .column, .columns {margin:0px 10px 40px;} .accordion .answer .one-second.column {width:47%;} .wrap .one-second.column {width:47%;} /*.column_column ul li {margin-bottom:2px;}*/ .single .post-header {margin-bottom:10px;} /* Appnote seach box*/ .widget_appnote_search_widget {clear:both; display:block; overflow:hidden;} .widget_appnote_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_appnote_search_widget .searchform {display:inline-block; float:left;} .widget_appnote_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_appnote_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_appnote_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_appnote_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*flat box*/ .flat_box .photo_wrapper .icon {display:none;} .flat_box .photo_wrapper img.photo {transform: translateX(0px)} .flat_box .desc_wrapper {margin-left:0px;} /*center images at user profile*/ .page-id-2504 .image_frame .image_wrapper {text-align:center} .page-id-2504.if-overlay .image_frame:hover .image_wrapper img, .page-id-2504.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .if-overlay .image_frame .image_wrapper .image_links {display:none;} /* Changes for ClickDimensions submit button */ a.button_theme, a.tp-button.button_theme, button, input[type="submit"], input[type="reset"], input[type="button"] { background-color: #0065a4 !important; color: #ffffff !important; font-family: inherit !important; /*padding:0px;*/ } /*isotope wrapper*/ .blog_wrapper.isotope_wrapper .post-item .post-footer {background:none;} .blog_wrapper.isotope_wrapper .post-item .post-footer .post-links{border:0px;} .blog_wrapper.isotope_wrapper .grid .post-photo-wrapper {border:0px;} /* product page*/ .page-id-2582 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-2582 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-2582.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-2582 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-2582 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-2582 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-2582 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-2582 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .uk-margin-small-right {vertical-align:middle; margin-top:-2px;} .page-id-2647 .ui-tabs .ui-tabs-panel .column.one-second {margin-bottom:0px;} .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {border-color:transparent;} @media only screen and (min-width: 960px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper {width:12%;} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:88%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-3641 .blog_wrapper .classic .post-photo-wrapper { width:25%} .page-id-3641 .blog_wrapper .classic .post-desc-wrapper {width:75%;} } @media only screen and (min-width: 768px) { .page-id-2640 .column.column_pricing_item {margin:0px 1% 40px;} } /* contact form 7 submit button*/ .wpcf7-form-control.wpcf7-submit {padding:10px 30px;} .wpcf7 .red {color:#990000;} .wpcf7 span.wpcf7-list-item {display:block; margin:0px;} .wpcf7 label {font-weight:400;} #lostusername {border:1px solid #ddd; padding:30px 20px 0px;} #lostusername .input {width:100%;} #lostusername .button {padding:10px 20px;} .ur-frontend-form button[type="submit"], .ur-frontend-form input[type="submit"] {float:none;} .page-id-2690.if-overlay .image_frame:hover .image_wrapper img, .page-id-2690.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-2690 .team .desc_wrapper hr {display:none;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-2690 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-2690 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* webinars seach box*/ .widget_webinars_search_widget {clear:both; display:block; overflow:hidden;} .widget_webinars_search_widget .widget-title {display:inline-block; float:left; margin-right:15px; margin-top:5px;} .widget_webinars_search_widget .searchform {display:inline-block; float:left;} .widget_webinars_search_widget .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .widget_webinars_search_widget .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .widget_webinars_search_widget .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .widget_webinars_search_widget .searchform input[type=submit] {margin:0px; margin-top:15px;} } /*Recent Webinars nav issue fix*/ .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-3665 li#menu-item-2421.current-menu-ancestor:not(.hover) a:after {display:none;} /*login-in nav issue fix*/ .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-5451 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* Contact Customer Support nav issue fix*/ .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-9343 li#menu-item-2390.current-menu-ancestor:not(.hover) a:after {display:none;} /* custom seach box*/ .search #Content .searchform {display:inline-block; float:left;} .search #Content .searchform h4{clear:both; padding:10px 0px; margin:0px;} .search #Content .searchform input[type=text] {float:left; margin:0px; min-width:350px; padding:7px 10px;} .search #Content .searchform select {-webkit-appearance:menulist; padding:7px 10px;} .search #Content .searchform input[type=submit] {background:#0065a4; margin:0px; margin-left:15px; padding:8px 20px;} @media only screen and (min-width: 320px) and (max-width: 650px) { .search #Content .searchform input[type=text] {min-width:240px;} } @media only screen and (min-width: 320px) and (max-width: 519px) { .search #Content .searchform input[type=submit] {margin:0px; margin-top:15px;} } .wpcf7-form .anr_captcha_field {position:absolute;} /*newsletter archive tables remove border*/ .post.category-newsletter-archive .post-wrapper-content table td {box-shadow:none; border:0px;} .be-ix-link-block {max-width:750px; text-align:center; margin:0px auto; padding-top:20px;} #Footer .widgets_wrapper .column {margin:0px 9px 0px;} @media only screen and (min-width: 768px) and (max-width: 959px) { #Footer .widgets_wrapper .column {margin:0px 5px 0px;} } /*Login page popup */ .page-id-5451.logged-in #pum-8728 {display:none !important;} #cookie-law-info-bar a {color:#fff; text-decoration:underline;} #cookie-law-info-bar a:hover {text-decoration:none;} #cookie-law-info-bar .cli-plugin-button {text-decoration:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { #cookie-law-info-again{display:none !important;} } /*After Multi Language SETUP*/ #Action_bar .column {overflow:visible;} .top-widgets-container .widget_search input[type="text"] {margin-top:5px;} .widget.widget_nav_menu::after, .widget.widget_icl_lang_sel_widget::after, .widget_archive ul li:after, .widget_nav_menu ul li:after {display:none;} .wpml-ls-legacy-dropdown-click {margin-top:6px; } .wpml-ls-legacy-dropdown-click a, .wpml-ls-legacy-dropdown-click a:hover, .wpml-ls-legacy-dropdown-click a:focus, .wpml-ls-legacy-dropdown-click .wpml-ls-current-language:hover>a {color:#626262 !important; border:0px;text-decoration:none;} .wpml-ls-legacy-dropdown-click .wpml-ls-sub-menu {z-index:999;} .top-widgets-container h2.title {display:none;} @media only screen and (min-width: 320px) and (max-width: 767px) { .wpml-ls-legacy-dropdown-click {margin:6px auto 0px; } .top-widgets-container .widget_search {clear:both;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .wpml-ls-legacy-dropdown-click {width:auto;} } #menu-top-navigation-ch .cart a {padding:0px 20px; } #menu-top-navigation-ch .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:10px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ch .cart.menu-item-2449 {display:none;} #menu-top-navigation-ch .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-13087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-13087 > a, #Top_bar.is-sticky .menu > li#menu-item-13087 > a span {line-height:30px; margin-top:0px;} } .post-9911 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .post-9911 span.wpcf7-form-control-wrap{margin-bottom:20px;} .post-9911 .wpcf7-list-item-label{color:#000000;} .post-9911 .unique .one-third.column{width:30.333% !important;} .post-9911 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .post-9911 .wrap .one-second.column{width:32%;} .post-9911 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .post-9911 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .post-9911 .wpcf7 span.wpcf7-list-item{width: 100%;} .post-9911 .unique .one-third.column{width:100% !important;} .post-9911 .wrap .one-second.column{width:100%;} .post-9911 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*remove meta from search-results*/ .search.search-results .post-item .post-meta {display:none;} .search.search-results .post-item .post-desc {padding-left:0px;} /*footer update*/ /*#Footer .widgets_wrapper {padding-bottom:10px;} #Footer .widgets_wrapper .column {margin-bottom:10px;} #Footer #custom_html-4 > h4 {text-align:center;} #Footer #custom_html-14 > h4 {text-align:center;} #Footer #custom_html-9 {max-width:160px; margin:0px auto; clear:both; padding-top:25px;} .footer-resources {max-width:80%; margin:0px auto; } .footer-resources li {float:left; line-height:28px;} .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:12%; clear:both;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:28%;} html[lang="zh-hans"] .footer-resources li:nth-child(1), html[lang="zh-hans"] .footer-resources li:nth-child(6){width:15%; clear:both;} html[lang="zh-hans"] .footer-resources li:nth-child(2), html[lang="zh-hans"] .footer-resources li:nth-child(7){width:25%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:17%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:18%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:25%;} @media only screen and (min-width:320px) and (max-width:1239px) { .footer-resources {max-width:60%; } .footer-resources li:nth-child(1), .footer-resources li:nth-child(6){width:50%; clear:none;} .footer-resources li:nth-child(2), .footer-resources li:nth-child(7){width:50%;} .footer-resources li:nth-child(3), .footer-resources li:nth-child(8){width:50%;} .footer-resources li:nth-child(4), .footer-resources li:nth-child(9){width:50%;} .footer-resources li:nth-child(5), .footer-resources li:nth-child(10){width:50%;} } @media only screen and (min-width:320px) and (max-width:767px) { .footer-resources {max-width:100%; } #Footer #custom_html-4 > h4 {text-align:left;} #Footer #custom_html-14 > h4 {text-align:left;} #Footer #custom_html-9 {max-width:100%;} } */ .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display:inline-block; text-decoration: none; padding: 0px 20px !important; border:0px; text-transform: uppercase; line-height:30px; font-size: 15px; font-weight: 400;letter-spacing: 1px;} @media only screen and (min-width:320px) and (max-width:767px) { .tabs_vertical.ui-tabs .ui-tabs-nav li h3, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active h3 {text-align:center;} .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover {margin:20px auto;} } /*Webinars latest posts zh-hans*/ .parent-pageid-11725 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .parent-pageid-11725 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .parent-pageid-11725 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .parent-pageid-11725 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .parent-pageid-11725 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .parent-pageid-11725 .Latest_news ul li .desc_footer .date {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .parent-pageid-11725 .Latest_news ul li .desc_footer .post-comments {display:none;} .parent-pageid-11725 .Latest_news ul li .desc_footer .button-love {display:none;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .parent-pageid-11725 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Upcoming Live Webinars zh-hans*/ .page-id-11764 .Latest_news ul li {margin-top:15px; margin-bottom:15px;} .page-id-11764 .Latest_news ul li.has-post-thumbnail .desc {margin-left: 70px; width: 95%;} .page-id-11764 .Latest_news ul li .photo {width:70px; border-right:0px; text-align:center; display:table;} .page-id-11764 .Latest_news ul li .photo a {display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li .photo img {max-width:50px !important; vertical-align:middle;} .page-id-11764 .Latest_news ul li .desc {border-left:3px solid #000; padding-top:0px; padding-bottom:0px; display:table-cell; vertical-align:middle;} .page-id-11764 .Latest_news ul li.category-webinars-zh-hans .desc h5 a{color:#007dc5;} .page-id-11764 .Latest_news ul li .desc_footer .date {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .icon-comment-empty-fa {display:none} .page-id-11764 .Latest_news ul li .desc_footer .post-comments {display:none;} .page-id-11764 .Latest_news ul li .desc_footer .button-love {display:none;} .page-id-11764 .Latest_news ul li .desc .post-excerpt {margin-top:0px;} .page-id-11764 .Latest_news ul li .desc .post-excerpt p{margin-bottom:0px; line-height:20px;} /*Request Product Quote zh-hans*/ .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12227 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12227 .wpcf7-list-item-label{color:#000000;} .page-id-12227 .unique .one-third.column{width:29% !important;} .page-id-12227 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12227 .wrap .one-second.column{width:32%;} .page-id-12227 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12227 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12227 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12227 .unique .one-third.column{width:100% !important;} .page-id-12227 .wrap .one-second.column{width:100%;} .page-id-12227 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Request Product Info zh-hans*/ .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 260px;display: inline-block;} .page-id-12612 span.wpcf7-form-control-wrap{margin-bottom:20px;} .page-id-12612 .wpcf7-list-item-label{color:#000000;} .page-id-12612 .unique .one-third.column{width:29% !important;} .page-id-12612 .unique .required{color: #ff0000; font-size: 13px; padding: 0 5px 0 5px;} .page-id-12612 .wrap .one-second.column{width:32%;} .page-id-12612 .wpcf7 hr.small {color:#000000;background-color:#000000;height:1px; max-width: 440px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 hr.large{color:#000000;background-color:#000000;height:1px; max-width: 760px;margin-bottom: 15px;margin-left:0px;margin-top:25px;} .page-id-12612 .wpcf7 p strong{font-weight:normal; font-size:18px; color:#1F497D;} @media only screen and (min-width: 320px) and (max-width: 959px) { .page-id-12612 .wpcf7 span.wpcf7-list-item{width: 100%;} .page-id-12612 .unique .one-third.column{width:100% !important;} .page-id-12612 .wrap .one-second.column{width:100%;} .page-id-12612 span.wpcf7-form-control-wrap {margin-bottom:0px;} } /*Team in zh-hans*/ .page-id-12002.if-overlay .image_frame:hover .image_wrapper img, .page-id-12002.if-overlay .image_frame.hover .image_wrapper img {transform: scale(1);} .page-id-12002 .team .desc_wrapper hr {display:none;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-second, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth, .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-third, .page-id-12002 .ui-tabs .ui-tabs-panel .column.two-third {margin-left:10px; margin-right:10px;} .page-id-12002 .ui-tabs .ui-tabs-panel .column.one-fourth {width:23.4%;} /* product page in zh-hans*/ .page-id-12156 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-12156 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-12156.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-12156 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-12156 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-12156 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-12156 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-12156 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } /*Recent Webinars nav issue fix for zh-hans*/ .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a {color:#000000;} .page-id-11728 li#menu-item-13052.current-menu-ancestor:not(.hover) a:after {display:none;} .large h3{font-size:22px; line-height:30px;} .small {margin-left:25px;} sup {display:inline-block; margin-top:-4px;} sub {display:inline-block; margin-bottom:-4px;} sup, sub {display:inline-table;} .ui-tabs-panel .one-second.column {width:50%;} .parent-pageid-2582 .ui-tabs-panel td {text-align:left !important;} .ls-borderlessdark3d .ls-shadow { display: none !important;} #menu-top-navigation-de .cart a {padding:0px 20px;} #menu-top-navigation-de .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-de .cart.menu-item-14543{display:none;} #menu-top-navigation-de .cart a {padding:0px 10px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-15087 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-15087 > a, #Top_bar.is-sticky .menu > li#menu-item-15087 > a span {line-height:30px; margin-top:0px;} } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar li#menu-item-12984 a {color:#000 !important; padding-top:20px !important;} .mm-vertical #Top_bar li#menu-item-14978 a {color:#000 !important; padding-top:20px !important;} } #Footer #custom_html-15 > h4 {text-align:center;} /* product page German */ .page-id-14845 .ui-tabs-panel .team.team_vertical .image_frame {border:0px;} .page-id-14845 .ui-tabs-panel .team.team_vertical hr.hr_color {background:none;} .page-id-14845.if-overlay .ui-tabs-panel .team.team_vertical .image_frame:hover .image_wrapper img, .page-id-14295.if-overlay .ui-tabs-panel .team.team_vertical .image_frame.hover .image_wrapper img {-webkit-transform: scale(1); -moz-transform: scale(1); -o-transform: scale(1); transform: scale(1); } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-3) {margin-left:10px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-2) {margin-left:18px; margin-right:17px; } .page-id-14845 .ui-tabs-panel .one-third.column:nth-child(4n-1) {margin-left:18px; margin-right:10px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:65px; margin-right:52px; } .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even) {margin-left:53px; margin-right:65px; } @media only screen and (min-width: 1301px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:40.2%;} } @media only screen and (min-width: 1240px) and (max-width: 1300px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:30.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:47.6%;} } @media only screen and (min-width: 960px) and (max-width: 1239px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:29.9%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:36.6%;} } @media only screen and (min-width: 768px) and (max-width: 959px) { .page-id-14845 .ui-tabs-panel .one-third.column {width:28.7%;} .page-id-14845 .ui-tabs-panel .one-second.column {width:38.9%;} } @media only screen and (min-width: 320px) and (max-width: 767px) { .page-id-14845 .ui-tabs-panel .one-third.column {margin-left:0px; margin-right:0px;} .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(even), .page-id-14845 .ui-tabs-panel .one-second.column:nth-child(odd) {margin-left:0px; margin-right:0px;} } .parent-pageid-14845 .ui-tabs-panel td {text-align:left !important;} .button_align.align_left .button {text-align:left; padding:10px 0;} .button_align.align_left .as_tab {font-size: 18px; line-height: 20px; font-weight: 400; background:#fff; letter-spacing: 0px; color: #2991d6 !important; padding: 5px 10px 5px 20px;margin:0; } .button_align.align_left .button.as_tab:hover {text-decoration:underline !important; background-color:#fff!important;} .button_align.align_left .button.as_tab:after {display:none;} .button_align.align_left .button.as_tab.active {color: #000; border-left: 3px solid #2991d6; border-radius: 0px !important; padding: 5px 10px 5px 17px !important;} .button_align.align_left .button.as_tab .button_label {margin:0!important;} .button_align.align_left .button.as_tab_btn { background: #007dc5; color: #fff; border-radius: 10px !important; text-align: center; margin-left: 20px; margin-top: 20px; display: inline-block; text-decoration: none; padding: 0px 20px !important; border: 0px; text-transform: uppercase; line-height: 30px; font-size: 15px; font-weight: 400; letter-spacing: 1px; } /*Japan language*/ #Footer #custom_html-22 > h4 { text-align: center; } #menu-top-navigation-ja .cart a {padding:0px 20px;} #menu-top-navigation-ja .cart a:before {font-family: "mfn-icons"; content:'\e82d'; float:left; padding-right:15px; font-size:26px; color:#fff; text-indent:0;} @media only screen and (min-width:320px) and (max-width:767px) { #menu-top-navigation-ja .cart.menu-item-2449 {display:none;} #menu-top-navigation-ja .cart a {padding:0px 10px;} } .mm-vertical #Top_bar .menu > li#menu-item-18403 a span {background:#007dc5; color:#fff; border-radius:10px; line-height:40px; margin-top:8px; margin-left:20px;} .mm-vertical #Top_bar .menu > li#menu-item-18403 a:after {display:none;} #Top_bar.is-sticky .menu > li#menu-item-18403 > a, #Top_bar.is-sticky .menu > li#menu-item-18403 > a span {line-height:30px; margin-top:0px;} @media only screen and (min-width: 1240px) { #Top_bar #menu-german-main-menu > li > a span:not(.description) { padding: 0 3px; font-size:14px; } } body, ul.timeline_items, .icon_box a .desc, .icon_box a:hover .desc, .feature_list ul li a, .list_item a, .list_item a:hover, .widget_recent_entries ul li a, .flat_box a, .flat_box a:hover, .story_box .desc, .content_slider.carousel ul li a .title, .content_slider.flat.description ul li .desc, .content_slider.flat.description ul li a .desc, .post-nav.minimal a i { color: #333333 !important; } .column_column ul, .column_column ol, .the_content_wrapper:not(.is-elementor) ul, .the_content_wrapper:not(.is-elementor) ol { color: #333333 !important; } .hero { margin-top: 20px; } .bg-hero { background: radial-gradient(circle at 100% 0,#ebf1f2 0% 30%,#0e81a7 100%); } .hero-full { min-height: 450px; background-color: rgba(0, 0, 0, 0.5); } .column, .columns { margin: 0px 8px 40px; } .image-container { position: relative; display: inline-block; /* oder 'block' je nach Layout */ } .ribbon { position: absolute; top: 10px; /* Abstand von oben */ left: -20px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #FF5733; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 5px 15px; /* Innenabstand */ transform: rotate(-0deg); /* Dreht das Ribbon */ transform-origin: 0 0; /* Setzt den Drehpunkt */ font-size: 14px; /* Schriftgröße */ box-shadow: 2px 2px 5px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .event-badge { position: relative; margin-right: 10px; border-radius: 6px; top: 0px; /* Abstand von oben */ left: 0px; /* Verschiebung nach links, um das Ribbon schräg zu positionieren */ background-color: #007dc5; /* Farbe des Ribbons */ color: white; /* Textfarbe */ padding: 2px 8px; /* Innenabstand */ font-size: 10px; /* Schriftgröße */ box-shadow: 1px 1px 2px rgba(0,0,0,0.3); /* Schatten für 3D-Effekt */ z-index: 10; /* Stellt sicher, dass das Ribbon über dem Bild liegt */ } .btn-default { background: #007dc5; color: #fff; border-radius: 6px; line-height: 40px; margin-top: 8px; margin-left: 20px; padding: 0px 10px; } .wpcf7 form.sent .wpcf7-response-output { color: #ffffff; background-color: #46b450; } .jumpto { padding-top: 100px; /* Adjust this value to match your header's height */ margin-top: -100px; /* This should be the same value as padding-top but negative */ } .post-item { margin-bottom: 40px; position: relative; float: left; width: 100%; border: 1px solid #ccc; padding: 20px; background-color: #f8f8f8; } .post-links { background-color: #007dc5; border: 1px solid #ccc; padding: 5px 20px; } #field_1_18, #field_1_113, #field_1_20, #field_1_21, #field_1_22, #field_1_23, #field_1_24, #field_1_25, #field_1_26, #field_1_27, #field_1_49, #field_1_51, #field_1_52, #field_1_53, #field_1_54, #field_1_56, #field_1_57, #field_1_58, #field_1_59, #field_1_60, #field_1_62, #field_1_63, #field_1_64, #field_1_65, #field_1_67, #field_1_68, #field_1_70, #field_1_72, #field_1_98, #field_1_99, #field_1_100, #field_1_101, #field_1_102, #field_1_103, #field_1_104, #field_1_107, #field_1_108, #field_1_122, #field_1_123, #field_1_124, #field_1_125, #field_1_126, #field_1_127, #field_1_128, #field_1_136 { background-color: #E8E8E8; padding: 20px 30px; border: 1px solid #C4C4C4; border-bottom: 1px solid #C4C4C4; margin-top: -20px; } #field_1_73, #field_1_74, #field_1_75, #field_1_76, #field_1_77, #field_1_78, #field_1_82, #field_1_83, #field_1_84, #field_1_85, #field_1_86, #field_1_92, #field_1_93, #field_1_94, #field_1_95, #field_1_129, #field_1_132, #field_1_133, #field_1_134, #field_1_135 { background-color: #F8F8F8; padding: 20px 50px; border-bottom: 1px solid #C4C4C4; border-right: 1px solid #C4C4C4; border-left: 1px solid #C4C4C4; margin-top: -40px; } #field_1_114, #field_1_115, #field_1_116, #field_1_117, #field_1_118, #field_1_119, #field_1_120, #field_1_121, #field_1_130 { background-color: #8ed1fc; padding: 20px 50px; border-bottom: 1px solid #C4C4C4; border-right: 1px solid #C4C4C4; border-left: 1px solid #C4C4C4; margin-top: -40px; } .gfield_label { font-weight: 800 !important; font-size: 16px !important; } .post-more, .post-more:hover, .icon-doc-text { color: #ffffff; } .icon_box.has_border { background: #fff; border: 0px solid #e1e1e1 !important; padding: 40px 10%; padding-bottom: 40px; } #Footer .widget > h4 { font-size: 18px; font-weight: 600; margin-bottom: 20px; } /* new css */ .mcb-column-inner { margin: 0px; overflow:visible;} .one-sixth.column { width: 14.666%; } /* 1/6 */ .one-fifth.column { width: 18%; } /* 1/5 */ .one-fourth.column, .four.columns { width: 23%; } /* 1/4 */ .one-third.column { width: 31.333%; } /* 1/3 */ .two-fifth.column { width: 38%; } /* 2/5 */ .one-second.column { width: 48%; } /* 1/2 */ .three-fifth.column { width: 58%; } /* 3/5 */ .two-third.column { width: 64.666%; } /* 2/3 */ .three-fourth.column { width: 73%; } /* 3/4 */ .four-fifth.column { width: 78%; } /* 4/5 */ .five-sixth.column { width: 81.333%; } /* 5/6 */ .one.column { width: 98%; } /* 1/1 */ .wpcf7-form .column { margin-bottom: 10px;} .wpcf7-form .column { padding: 0px;} .wpcf7-form .column p{ margin: 0px;} .column-margin-0px.column { margin-bottom: 0 !important;} .column-margin-10px.column { margin-bottom: 0px !important;} .column-margin-20px.column { margin-bottom: 0px !important;} .page-pager, .post-pager { margin-bottom: 0!important;} .column.column_divider { margin-bottom: 0!important; height: auto!important;} #Header .container {padding-left:0; padding-right:0;} .accordion .question > .title > .acc-icon-plus, .accordion .question.active > .title > .acc-icon-minus { display: block;} .accordion .question > .title > .acc-icon-plus, .accordion .question .title > .acc-icon-minus {left:15px; top:16px;} .mcb-wrap-inner {display:block;} @media only screen and (max-width: 767px) { .section_wrapper .mcb-wrap, .section_wrapper .column, .container .column { width:100%!important; } } @media only screen and (min-width: 768px) { .be-ix-link-block .be-related-link-container .be-label {flex:0 0 17%;} .be-ix-link-block .be-related-link-container .be-list {flex:0 0 83%;} } ul.clients.appnotes .client_wrapper a, ul.clients.appnotes .client_wrapper .gs-wrapper { justify-content:left; } /*Top bar search*/ #Action_bar #global-searchfield-container .addsearch-searchfield input[type=search].icon {margin-bottom:0px; margin-top:4px; padding:5px 5px 5px 40px!important; height:30px;} #Action_bar #global-searchfield-container .addsearch-searchfield button {display:none;} #Action_bar .adds-components {padding:0px;} /*Top Shadow */ .header-classic #Top_bar { border-bottom: 0px solid #e1e1e1; box-shadow: rgba(0, 0, 0, 0.15) 0px 1.95px 2.6px; margin-bottom: 1px; } .sticky-submenu { position: -webkit-sticky; /* Für Safari */ position: sticky; top: 80px; z-index: 9999; /*background-color: #fff;*/ padding: 10px; /*border-bottom: 1px solid #ccc;*/ text-align: center; } .sticky-submenu ul { list-style-type: none; margin: 0; padding: 0; } .sticky-submenu ul li { display: inline; margin: 0 15px; } .sticky-submenu ul li a { text-decoration: none; /*color: #000;*/ color:#fff; padding: 5px; transition: border-bottom 0.3s; } .sticky-submenu ul li a.active, .sticky-submenu ul li a:hover { /*border-bottom: 2px solid #000;*/ border-bottom: 2px solid #fff; } .page-section { padding: 50px; margin-top: 50px; } .anchor-offset { display: block; position: relative; top: -60px; /* Höhe des sticky-submenu */ } .copyright a {padding:0px 7px;} .before-footer h4 {font-size:18px;} .before-footer .button {border-radius:4px;} .footer-button { padding: 10px 5px; border-radius: 5px; text-align: center; display: inline-block; width: 180px; border:1px solid #fff !important;} #Footer .widget_nav_menu ul { list-style-type: none; padding: 0px; background: none;} #Footer .widget_nav_menu ul a{padding:0px;} #Footer .widget_nav_menu {margin:20px 0px;} #Footer .one-fifth {width:16%;} #Footer .one-fifth:first-child {width:27%;} .social .icon_bar, .social .icon_bar span {width:auto !important; height:auto!important; line-height:0px!important; background-color:transparent; border:0px !important; color:#fff; font-size:24px; -webkit-box-shadow:none;box-shadow:none;} .social a:hover.icon_bar span { -webkit-transform: translateY(0%); -moz-transform: translateY(0%); transform: translateY(0%); } /*30aug2024*/ table tr:first-child td {box-shadow:none;} .btn_radius {border-radius: 4px;} #Top_bar .menu > li > a span:not(.description) {padding:0px 3px;} @media only screen and (min-width: 1300px) { #Top_bar .menu > li > a span:not(.description) {padding:0px 10px;} #Top_bar.is-sticky .menu > li > a span {padding:0px 15px;} } /** Added 11/14/24 **/ .scroll { text-decoration: none; transition: color 0.3s ease, font-weight 0.3s ease; } .scroll.active { font-weight: bold; } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-5221 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-15087 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-13087 a span { background: #13b5ea !important; } } @media only screen and (min-width: 1240px) { .mm-vertical #Top_bar .menu > li#menu-item-18403 a span { background: #13b5ea !important; } } /** Added 11/18/24 **/ .header-section { border-bottom: 8px solid #fdbb30 !important; } .header-section .mcb-background-overlay{ } .solution-box { padding: 20px; border: 4px solid #EBEDEF; min-height: 150px; } .image_frame:hover .image_wrapper .mask { display:none; } .image_frame:hover .image_wrapper img, .image_frame:focus .image_wrapper img { top: 0 !important; -webkit-transform: scale(1) !important; -moz-transform: scale(1) !important; -o-transform: scale(1) !important; transform: scale(1) !important; -webkit-transition: none !important; transition: none !important; } .section-headline { border-bottom: thick solid #fdbb30; padding-bottom: 15px; font-size: 26px; } /** Page CTA Elements **/ .responsive-container { display: flex; flex-wrap: nowrap; border: 4px solid #EBEDEF; padding: 20px; align-items: flex-start; gap: 20px; width: 100%; } .responsive-container img { width: 100px; height: auto; flex-shrink: 0; } .responsive-container div { flex: 1; } /* Breakpoint for mobile screens */ @media (max-width: 768px) { .responsive-container { flex-wrap: wrap; } .responsive-container img { width: 100%; margin-bottom: 20px; } .responsive-container div { flex: 1 1 100%; text-align: center; } } .cta-box-btn { display: inline-block; align-items: center; padding: 6px 20px; color: white; text-decoration: none; border-radius: 5px; font-size: 14px; width: fit-content; background-color: #0065a4 !important; transition: background-color 0.3s ease, transform 0.2s ease; } .cta-box-btn:hover { background-color: #0056b3; color: white; text-decoration: none; } .cta-box-icon { margin-right: 5px; } /* Sidebar-Button-Styling */ .sidebar-btn { display: block; width: 100%; text-align: center; padding: 10px 20px; box-sizing: border-box; background-color: #13b5ea !important; font-size: 16px; color: white; border-radius: 5px; transition: background-color 0.3s ease, transform 0.2s ease; } .sidebar-btn:hover { background-color: #0065a4 !important; color: white; text-decoration: none; } /*LS nav style*/ html body .ls-bottom-nav-wrapper a, #ls-global .ls-bottom-nav-wrapper a {background-image:none; border-radius:20px; background-color:#999; margin:3px; width:15px; height:15px;} html body .ls-bottom-nav-wrapper a.v, #ls-global .ls-bottom-nav-wrapper a.ls-nav-active {background-color:#2991d6;} .tabs_vertical.ui-tabs .ui-tabs-nav li a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button, .tabs_vertical.ui-tabs .ui-tabs-nav li a.button:hover, .tabs_vertical.ui-tabs .ui-tabs-nav li.ui-tabs-active a.button:hover { background: #007dc5; color: #fff; border-radius: 8px !important; text-align: center; margin-left: 20px; margin-top: 17px; display: inline-block; text-decoration: none; padding: 5px 12px !important; border: 0px; text-transform: uppercase; line-height: 30px; font-size: 16px; font-weight: 500; letter-spacing: 1px; width: 80%; } /* Marketo Forms */ .mktoForm, .mktoForm .mktoFieldWrap, .mktoForm .mktoHtmlText, .mktoForm input, .mktoLogicalField .mktoCheckboxList, .mktoRangeField, .mktoRangeValue {width:100% !important;} @media only screen and (min-width:480px) { .mktoFormCol:first-child:nth-last-child(2), .mktoFormCol:first-child:nth-last-child(2) ~ .mktoFormCol {width: 100% 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</div> <div class="banner_wrapper"> </div> <div class="search_wrapper">  <form role="search" method="get" id="searchform2" class="searchform" action="https://www.wyatt.com/" > <div><input type="text" placeholder="Search..." value="" name="s" id="s" /> <a href="javascript:document.getElementById('searchform2').submit();" class="icon-search-fine"></a> </div> </form><div class="mobilecart"><a href="https://store.wyatt.com/"></a></div> </div> </div> </div> </div> </div> </header> </div>  <div id="Content" role="main"> <div class="content_wrapper clearfix"> <main class="sections_group"> <div class="entry-content" itemprop="mainContentOfPage"> <div class="mfn-builder-content mfn-default-content-buider"><section class="section mcb-section mfn-default-section mcb-section-3aa5b3f01" style="" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-3aa5b3f01"><div class="wrap mcb-wrap mcb-wrap-d29e9d819 one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-d29e9d819 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-9f12dc75a one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-9f12dc75a mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><br></div></div></div></div></div></div></section><section class="section mcb-section mfn-default-section mcb-section-b6fc446c2" style="padding-bottom:30px" ><div class="mcb-background-overlay"></div><div class="section_wrapper mfn-wrapper-for-wraps mcb-section-inner mcb-section-inner-b6fc446c2"><div class="wrap mcb-wrap mcb-wrap-9d481c4bb one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-9d481c4bb mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-47c98103b one laptop-one tablet-one mobile-one column_column column-margin-10px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-47c98103b mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><h1>Understanding Dynamic Light Scattering</h1></div></div></div></div></div><div class="wrap mcb-wrap mcb-wrap-kpjfr4hnd one-second tablet-one-second laptop-one-second mobile-one valign-top clearfix" data-desktop-col="one-second" data-laptop-col="laptop-one-second" data-tablet-col="tablet-one-second" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-kpjfr4hnd mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-970438230 one laptop-one tablet-one mobile-one column_column column-margin-0px" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-970438230 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p>When in solution, macromolecules are buffeted by the solvent molecules. This leads to a random motion of the molecules called Brownian motion. For example, consider this movie of 2 µm diameter particles in pure water. As can be seen, each particle is constantly moving, and its motion is uncorrelated with the other particles. (Movie courtesy of <a href="http://www.physics.emory.edu/faculty/weeks.html" target="_blank" rel="noopener">Dr. Eric R. Weeks</a>, Emory University).</p> <p align="center"><img style="margin: 10px 0 10px 0;" src="/wp-content/uploads/2019/05/brownian-motion.gif" alt="Brownian motion" border="0" /></p> <p style="color: #075c97; font-size: 14px;" align="center">Brownian motion.</p> <p>As light scatters from the moving macromolecules, this motion imparts a randomness to the phase of the scattered light, such that when the scattered light from two or more particles is added together, there will be a changing constructive or destructive interference. This leads to time-dependent fluctuations in the intensity of the scattered light (Fig. 1).</p> <p>In dynamic light scattering (DLS), the time-dependent fluctuations in the scattered light are measured by a single photon counting module. The rate of fluctuations is directly related to the rate of diffusion of the molecule through the solvent, which is related in turn to the particles' hydrodynamic radii. Smaller particles diffuse faster, causing more rapid fluctuations in the intensity than larger particles. Therefore, the fluctuation in light intensity contains information about the diffusion of the molecules and can be used to extract a diffusion coefficient and calculate a particle size.</p> <p>DLS is employed by the <a href="/products/instruments/dynapro-nanostar-dynamic-light-scattering-detector.html">DynaPro<sup>™</sup> NanoStar<sup>™</sup></a>, the <a href="/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html">DynaPro<sup>™</sup> Plate Reader</a>, the <a href="/products/instruments/zetastar.html">ZetaStar™</a> and the <a href="https://store.wyatt.com/shop/udawn/udawn3/wiq/">WyattQELS<sup>™</sup> module</a> for MALS detectors to determine the effective particle size. The analyte’s translational diffusion coefficient, <i>D</i><sub>t</sub>, is obtained by automated nonlinear least squares fitting of the autocorrelation function that quantitatively describes the measured time-dependent fluctuations in light scattering intensity. The analysis is done directly in the accompanying <a href="/products/software/dynamics.html">DYNAMICS<sup>™</sup></a>, <a href="/products/software/dynamics-touch.html">DYNAMICS Touch<sup>™</sup></a> or <a href="/products/software/astra.html">ASTRA<sup>™</sup></a> software. A typical autocorrelation function for a monodisperse sample is shown in Fig. 2.</p> <p style="line-height: 150%;">The Stokes-Einstein equation then gives the hydrodynamic radius, <i>R</i><sub>h</sub>, (Fig. 3) corresponding to the measured <i>D</i><sub>t</sub>:</p> <p align="center"><img style="margin: 10px 0 10px 0; height: 75px;" src="/wp-content/uploads/2021/10/dls-theory-equation-1.jpg" alt="Equation 1" border="0" /></p> <p>where <i>k</i> is Boltzmann's constant, <i>T</i> is the temperature in K, and <i><span style="font-family: Symbol;">h</span></i> is the solvent viscosity.</p> <p>Different sizing techniques, e.g., DLS, small angle X-ray scattering, microscopy, and molecular modeling may report different types of radii. It is therefore important to know how a reported “size” was determined and whether it refers to the radius or diameter of the molecule. The <i>R</i><sub>h</sub> measured by DLS is the radius of a hard sphere with the same diffusion coefficient as the sample. Other measures include <i>R</i><sub>g</sub> (the radius of gyration, or root-mean-square radius, obtained by e.g., <a href="/library/theory/understanding-multi-angle-static-light-scattering.html">MALS</a>), <i>R</i><sub>vol</sub> (the radius of a hypothetical sphere that occupies the same volume as the macromolecule), and <i>R</i><sub>Rot</sub> (the radius subtended by rotating the macromolecule). Fig. 3 illustrates how these different measures compare for the compact, globular protein lysozyme.</p></div></div></div></div></div><div class="wrap mcb-wrap mcb-wrap-seog7m6wm one-second tablet-one-second laptop-one-second mobile-one valign-top clearfix" data-desktop-col="one-second" data-laptop-col="laptop-one-second" data-tablet-col="tablet-one-second" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-seog7m6wm mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-af8kbbtwh one laptop-one tablet-one mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-af8kbbtwh mcb-item-image-inner"><div class="image_frame image_item scale-with-grid aligncenter no_border" role="link" aria-label="Image with links" tabindex="0"><div class="image_wrapper"><a href="https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500.jpg" rel="prettyphoto" class="" tabindex="-1" title=""><div class="mask"></div><img width="500" height="464" src="https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500.jpg" class="scale-with-grid" alt="" style="" decoding="async" fetchpriority="high" srcset="https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500.jpg 500w, https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500-300x278.jpg 300w, https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500-157x146.jpg 157w, https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500-50x46.jpg 50w, https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500-81x75.jpg 81w" sizes="(max-width:767px) 480px, 500px" /></a><div class="image_links"><a href="https://www.wyatt.com/wp-content/uploads/2021/10/Constructive-vs-Destructive-Interference-DLS-500.jpg" class="zoom" rel="prettyphoto" tabindex="-1" title=""><svg viewBox="0 0 26 26" aria-label="zoom image"><defs><style>.path{fill:none;stroke:#333;stroke-miterlimit:10;stroke-width:1.5px;}</style></defs><circle cx="11.35" cy="11.35" r="6" class="path"/><line x1="15.59" y1="15.59" x2="20.65" y2="20.65" class="path"/></svg></a></div></div></div> </div></div><div class="column mcb-column mcb-item-67q42fb32 one laptop-one tablet-one mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-67q42fb32 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p align="left" style="color:#075c97;font-size:14px;padding-left:20px">Figure 1. Brownian motion results in measurable fluctuations in the light intensity over time.</p> </div></div></div><div class="column mcb-column mcb-item-x0p4eirbp one laptop-one tablet-one mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-x0p4eirbp mcb-item-image-inner"><div class="image_frame image_item scale-with-grid aligncenter no_border" role="link" aria-label="Image with links" tabindex="0"><div class="image_wrapper"><a href="https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500.png" rel="prettyphoto" class="" tabindex="-1" title=""><div class="mask"></div><img width="500" height="315" src="https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500.png" class="scale-with-grid" alt="" style="" decoding="async" srcset="https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500.png 500w, https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500-300x189.png 300w, https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500-232x146.png 232w, https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500-50x32.png 50w, https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500-119x75.png 119w" sizes="(max-width:767px) 480px, 500px" /></a><div class="image_links"><a href="https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-1-500.png" class="zoom" rel="prettyphoto" tabindex="-1" title=""><svg viewBox="0 0 26 26" aria-label="zoom image"><defs><style>.path{fill:none;stroke:#333;stroke-miterlimit:10;stroke-width:1.5px;}</style></defs><circle cx="11.35" cy="11.35" r="6" class="path"/><line x1="15.59" y1="15.59" x2="20.65" y2="20.65" class="path"/></svg></a></div></div></div> </div></div><div class="column mcb-column mcb-item-5q55cl175 one laptop-one tablet-one mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-5q55cl175 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p align="left" style="color:#075c97;font-size:14px;padding-left:20px">Figure 2. Autocorrelation function for a monodisperse sample with 50 nm <i>R</i><sub>h</sub> particles.</p> </div></div></div><div class="column mcb-column mcb-item-4rw51b54s one laptop-one tablet-one mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-4rw51b54s mcb-item-image-inner"><div class="image_frame image_item scale-with-grid aligncenter no_border" role="link" aria-label="Image with links" tabindex="0"><div class="image_wrapper"><a href="https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-2-500.png" rel="prettyphoto" class="" tabindex="-1" title=""><div class="mask"></div><img width="933" height="402" src="https://www.wyatt.com/wp-content/uploads/2023/01/fig-3-dls-theory.jpg" class="scale-with-grid" alt="" style="" decoding="async" srcset="https://www.wyatt.com/wp-content/uploads/2023/01/fig-3-dls-theory.jpg 933w, https://www.wyatt.com/wp-content/uploads/2023/01/fig-3-dls-theory-300x129.jpg 300w, https://www.wyatt.com/wp-content/uploads/2023/01/fig-3-dls-theory-768x331.jpg 768w, https://www.wyatt.com/wp-content/uploads/2023/01/fig-3-dls-theory-260x112.jpg 260w, https://www.wyatt.com/wp-content/uploads/2023/01/fig-3-dls-theory-50x22.jpg 50w, https://www.wyatt.com/wp-content/uploads/2023/01/fig-3-dls-theory-150x65.jpg 150w" sizes="(max-width:767px) 480px, (max-width:933px) 100vw, 933px" /></a><div class="image_links"><a href="https://www.wyatt.com/wp-content/uploads/2021/10/dls-theory-figure-2-500.png" class="zoom" rel="prettyphoto" tabindex="-1" title=""><svg viewBox="0 0 26 26" aria-label="zoom image"><defs><style>.path{fill:none;stroke:#333;stroke-miterlimit:10;stroke-width:1.5px;}</style></defs><circle cx="11.35" cy="11.35" r="6" class="path"/><line x1="15.59" y1="15.59" x2="20.65" y2="20.65" class="path"/></svg></a></div></div></div> </div></div><div class="column mcb-column mcb-item-5m363dwg5 one laptop-one tablet-one mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-5m363dwg5 mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><p align="left" style="color:#075c97;font-size:14px;padding-left:20px">Figure 3. (Left) <i>R</i><sub>h</sub> is the radius of a hard sphere with the same diffusion coefficient as the sample. (Right) Compact molecules, e.g., globular proteins such as lysozyme, generally have <i>R</i><sub>g</sub> < <i>R</i><sub>h</sub>.</p> </div></div></div></div></div><div class="wrap mcb-wrap mcb-wrap-engowg58l one tablet-one laptop-one mobile-one valign-top clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="padding:;background-color:" ><div class="mcb-wrap-inner mcb-wrap-inner-engowg58l mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-qbr4bi593 one laptop-one tablet-one mobile-one column_accordion" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-qbr4bi593 mcb-item-accordion-inner"><div class="accordion"><div class="mfn-acc accordion_wrapper "><div class="question"><div class="title"><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Analyzing Particle Size Distributions</h3></div><div class="answer"><div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Batch DLS can determine size distributions and the presence of multiple populations in a sample without the need for chromatographic separation, making it an excellent tool for rapid quality control and high-throughput screening of large numbers of samples. Such batch measurements of samples with a distribution of <i>R</i><sub>h</sub> and a corresponding distribution of diffusion times will yield autocorrelation functions described by the sum of the autocorrelation functions of all particle species in the sample weighed by their light scattering intensities. Wyatt Technology’s <a href="/products/software/dynamics.html">DYNAMICS<sup>™</sup></a>, <a href="/products/software/dynamics-touch.html">DYNAMICS<sup>™</sup> Touch<sup>™</sup></a> and <a href="/products/software/astra.html">ASTRA<sup>™</sup></a> software programs incorporate two methods for extracting additional information from batch measurements of such complex samples: Cumulants and Regularization (Fig. 4).</p> <p>The Cumulants method assumes one population of particles with a single average diffusion coefficient and a single standard deviation about that average. The algorithm can fit monomodal samples that are monodisperse or polydisperse (Fig. 4), such as nanoparticles or protein samples with populations of monomers, dimers and small oligomers.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px"> <p align="center"><a rel="lightbox" href="/wp-content/uploads/2021/10/dls-theory-figure-3.png"><img src="/wp-content/uploads/2021/10/dls-theory-figure-3.png" align="center"></a></p> <p align="left" style="color:#075c97;font-size:14px">Figure 4. Types of samples, the fits applicable to these samples and a typical Regularization graph are shown.</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>The Regularization method, in contrast, assumes the presence of any number of populations of particles, each with its own diffusion coefficient, polydispersity, and standard deviation (i.e., the underlying distribution of <i>R</i><sub>h</sub> is smooth). Regularization can resolve species that differ by more than 3-5 times in size. This makes it the method of choice for identifying large aggregates and insoluble species in protein samples.</p> <p>For each peak or species, Regularization analysis provides a wide range of metrics, including:</p> <ul> <li>a size distribution histogram (examples shown in the bottom row of Table 1)</li> <li>mean radius</li> <li>% polydispersity</li> <li>molar mass estimated from the measured radius (<i>M</i><sub>w</sub>-R)</li> <li>the relative light intensity scattered by each population (%Intensity), estimated relative amount of mass (%Mass) or number of particles (%Number).</li> </ul> <p>Cumulants and Regularization fits and their corresponding residuals are displayed instantly following data acquisition in DYNAMICS and DYNAMICS Touch (Fig. 5), along with an automatic data quality assessment. This allows the user to quickly determine which measurements meet data quality standards, which need to be scrutinized or revisited, and which method provides the best fit.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px"> <p align="center"><a rel="lightbox" href="/wp-content/uploads/2021/10/dls-theory-fig-update-5.jpg"><img src="/wp-content/uploads/2021/10/dls-theory-fig-update-5.jpg" align="center"></a></p> <p align="left" style="color:#075c97;font-size:14px">Figure 5. (Left) Autocorrelation function of a multimodal sample (blue). (Middle) The Cumulants fit to the autocorrelation function (brown). (Right) The Regularization fit to the autocorrelation function (purple.) The Cumulants fit does not fit the autocorrelation function well for this multimodal sample.</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title"><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Measuring Nanoparticle Concentration</h3></div><div class="answer"><div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Taking advantage of the determination of hydrodynamic size from the diffusion behavior, and the total scattered intensity, another important property can be measured: the concentration of nanoparticles, <i>N</i>.</p> <p>The calculation requires the particle’s volume V and refractive index <i>n</i><sub>particle</sub>, and the refractive index of the solvent <i>n</i><sub>0</sub>. The assumption of a user-specified shape model (e.g., uniform sphere or coated sphere) is also required. Then the diffusion coefficient may be interpreted to obtain a dimension such as radius, from which the volume of the particle is calculated (Fig. 6). The refractive index of the particle’s constituent material may be found in the literature. The particle concentration per unit volume, <i>N</i>, can then be determined from the light scattering intensity quantified as the excess Rayleigh ratio at angle zero and the following equation (Van Holde, K. E. Physical biochemistry. Prentice-Hall, 1971):</p> <p align="center"><img src="/wp-content/uploads/2021/10/dls-theory-equation-2-75.jpg" align="center" height="75px"></p> <p>The maximum <i>R</i><sub>h</sub> for concentration measurements are 160 nm for the DynaPro Plate Reader and 175 nm for the NanoStar. Particles with larger sizes and more complex shapes require multi-angle light scattering (MALS) and can be probed with the <a href="/products/instruments/eclipse-field-flow-fractionation-system.html">Eclipse<sup>™</sup></a> FFF-MALS instrument.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px"> <p align="center"><a rel="lightbox" href="/wp-content/uploads/2021/10/dls-theory-fig-update-6.jpg"><img src="/wp-content/uploads/2021/10/dls-theory-fig-update-6.jpg" align="center"></a></p> <p align="left" style="color:#075c97;font-size:14px">Figure 6. DLS can be used to measure the particle concentration in a sample.</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title"><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Characterizing Intermolecular Interactions</h3></div><div class="answer"><div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>Nonspecific intermolecular interactions are found throughout nature and have multiple physical origins, including electrostatic attraction and repulsion, hydrophobic and excluded volume effects, and induced dipoles. Interactions can be attractive or repulsive, and are of interest in the formulation of biotherapeutics.</p> <p>Both the DynaPro NanoStar and DynaPro Plate Reader can quantify macromolecular interactions with DLS and/or SLS. DLS assesses nonspecific interactions and colloidal stability via the diffusion interaction parameter <i>k</i><sub>D</sub>. SLS quantifies the second virial coefficient (<i>A</i><sub>2</sub>). Both <i>k</i><sub>D</sub> and <i>A</i><sub>2</sub> represent complementary metrics of the solute-solute interaction in a specific solvent, and are useful both for understanding interactions and for ranking formulations.</p> <p>In an ideal dilute solution, the diffusion coefficient (<i>D</i><sub>t</sub>) measured by DLS is not dependent on solute concentration. As concentration increases, the solution becomes less ideal, and a first-order expansion is performed; the diffusion interaction parameter, <i>k</i><sub>D</sub> (with units of mL/g), is the first-order correction to the diffusion coefficient as follows:</p> <p align="center"><img src="/wp-content/uploads/2021/10/dls-theory-equation-3-60.jpg" align="center"></p> <p><i>k</i><sub>D</sub> is determined by measuring the diffusion coefficient as a function of concentration, in the concentration range where the first-order approximation applies (typically 1 – 10 mg/mL). </p> <p>Attractive interactions (<i>k</i><sub>D</sub> < 0) cause an apparent decrease in <i>D</i><sub>t</sub> and an apparent increase in <i>R</i><sub>h</sub>, while repulsive interactions (<i>k</i><sub>D</sub> > 0) cause an apparent increase in <i>D</i><sub>t</sub> and an apparent decrease in <i>R</i><sub>h</sub> (Fig. 7). Molecules behaving as hard spheres for which <i>A</i><sub>2</sub> is close to the excluded volume value typically have <i>k</i><sub>D</sub> values between 0 and 5 mL/g.</p> <p>The diffusion interaction parameter is not a purely thermodynamic quantity – it combines thermodynamic and hydrodynamic contributions. For a purely thermodynamic quantity describing colloidal interactions, the second virial coefficient is commonly explored.</p> <p>The second virial coefficient, <i>A</i><sub>2</sub>, obtained by SLS and MALS via measurements of scattered intensity versus concentration, also reports on solvent-dependent macromolecular self-association and colloidal stability. Specifically, attractive interactions (<i>A</i><sub>2</sub> < 0) cause the scattering intensity to increase faster than linear and yield an apparent increase in molar mass, and repulsive interactions (<i>A</i><sub>2</sub> > 0) cause the scattering intensity to increase slower than linear and yield an apparent decrease in molar mass (Fig. 8). At low concentrations typical of chromatography, the effect of <i>A</i><sub>2</sub> is negligible, and <i>A</i><sub>2</sub> can be set to 0 for data analysis.</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <div style="padding:0px 30px 0px 30px"> <p align="center"><a rel="lightbox" href="/wp-content/uploads/2021/10/dls-theory-fig-update-8.jpg"><img src="/wp-content/uploads/2021/10/dls-theory-fig-update-8.jpg" align="center"></a></p> <p align="left" style="color:#075c97;font-size:14px">Figure 7. Translational diffusion coefficient (left) and apparent <i>R</i><sub>h</sub> (right) as a function of concentration for <i>k</i><sub>D</sub> = 0 (solid blue line), <i>k</i><sub>D</sub>> 0 (dashed red line), and <i>k</i><sub>D</sub> < 0 (dotted green line).</p> </div> <br><br> <div style="padding:0px 30px 0px 30px"> <p align="center"><a rel="lightbox" href="/wp-content/uploads/2021/10/dls-theory-fig-update-7.jpg"><img src="/wp-content/uploads/2021/10/dls-theory-fig-update-7.jpg" align="center"></a></p> <p align="left" style="color:#075c97;font-size:14px">Figure 8. Light scattering intensity (left) and apparent molar mass (right) as a function of concentration for <i>A</i><sub>2</sub> = 0 (solid blue line), <i>A</i><sub>2</sub> > 0 (dashed red line), and <i>A</i><sub>2</sub> < 0 (dotted green line).</p> </div> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> <div class="question"><div class="title"><i class="icon-plus acc-icon-plus"></i><i class="icon-minus acc-icon-minus"></i><h3>Understanding the Autocorrelation Function</h3></div><div class="answer"><div class="column one-second mobile-one"><div class="mcb-column-inner"> <p>In DLS, the fluctuations in light intensity measured over time are quantified via a second order correlation function g<sup>(2)</sup> (τ). The function of intensity is shifted by a delay time (τ) and the autocorrelation function g(τ) is calculated. Qualitatively, the autocorrelation function is a measure of how similar the intensity function is to itself when shifted by time τ. As the value of τ increases, the function reaches a baseline of 1.</p> <p>More specifically, and as described in various light scattering texts (cf. Chu, B. Laser Light Scattering: Basic Principles and Practice; Academic Press: Boston, 1991), the correlation function for a monodisperse sample can be analyzed by the equation:</p> <p align="center"><img src="/wp-content/uploads/2021/10/dls-theory-equation-4-35px.jpg" align="center" height="50px"></p> <p>where β is the correlation function amplitude at zero delay, Γ is the decay rate, and the baseline of the correlation function relaxes to a value of 1 at infinite delay.</p> <p>DYNAMICS and ASTRA use a nonlinear least squares fitting algorithm to fit the measured correlation function to equation 2 to retrieve the correlation function decay rate Γ. From this point, Γ can be converted to the translational diffusion coefficient Dt for the particle via the relation:</p> <p align="center"><img src="/wp-content/uploads/2021/10/dls-theory-equation-5.jpg" align="center"></p> <p> Here, <i>q</i> is the magnitude of the scattering vector, and is given by:</p> <p align="center"><img src="/wp-content/uploads/2021/10/dls-theory-equation-6-50px.jpg" align="center" height="50px"></p> <p>where <i>n</i><sub>0</sub> is the solvent index of refraction, Λ0 is the vacuum wavelength of the incident light, and <i>q</i> is the scattering angle.</p> <p>Finally, the diffusion coefficient can be interpreted as the hydrodynamic radius <i>R</i><sub>h</sub> of a diffusing sphere via the Stokes-Einstein equation:</p> <p align="center"><img src="/wp-content/uploads/2021/10/dls-theory-equation-7.jpg" align="center"></p> <p>where <i>k</i> is Boltzmann's constant, T is the temperature in K, and <i><font face="Symbol">h</font></i> is the solvent viscosity (Fig. 3).</p> </div></div> <div class="column one-second mobile-one"><div class="mcb-column-inner"> <p> </p> </div></div> <hr class="no_line" style="margin: 0 auto 0px auto"/> </div></div> </div></div> </div></div><div class="column mcb-column mcb-item-ipgdx440s one-fourth laptop-one-fourth tablet-one-fourth mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-ipgdx440s mcb-item-column-inner"><div class="column_attr mfn-inline-editor clearfix" style=""><a class="button button_full_width button_size_2" href="/library/webinars/dls.html" style="background-color:#13b5ea!important;color:#ffffff;" target="_blank" title=""><span class="button_label">View DLS Webinars</span></a> </div></div></div><div class="column mcb-column mcb-item-aep6mjlf5 one-fourth laptop-one-fourth tablet-one-fourth mobile-one column_column" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-aep6mjlf5 mcb-item-column-inner"><div class="column_attr 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data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-e4301fefd mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="wrap mcb-wrap mcb-wrap-33d9f437d one tablet-one laptop-one mobile-one mfn-nested-wrap clearfix" data-desktop-col="one" data-laptop-col="laptop-one" data-tablet-col="tablet-one" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-33d9f437d mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-578adcd6b one laptop-one tablet-one mobile-one column_heading" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-578adcd6b mcb-item-heading-inner"><h2 class="title"><span style="color: #007dc5; font-weight: 600;">Dynamic Light Scattering Instruments</span><br><div style="margin-top: 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center;">Automated DLS and static light scattering (SLS) measured directly in standard microwell plate.</p></div></div></div><div class="column mcb-column mcb-item-42397d14c one laptop-one tablet-one mobile-one column_button" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-42397d14c mcb-item-button-inner"><a class="button button_theme button_size_2" href="https://www.wyatt.com/products/instruments/dynapro-dynamic-light-scattering-plate-reader.html" title=""><span class="button_label">Learn More</span></a> </div></div></div></div><div class="wrap mcb-wrap mcb-wrap-5b5ef277a one-third tablet-one-third laptop-one-third mobile-one mfn-nested-wrap clearfix" data-desktop-col="one-third" data-laptop-col="laptop-one-third" data-tablet-col="tablet-one-third" data-mobile-col="mobile-one" style="" ><div class="mcb-wrap-inner mcb-wrap-inner-5b5ef277a mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column 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style="" ><div class="mcb-wrap-inner mcb-wrap-inner-035fae6a7 mfn-module-wrapper mfn-wrapper-for-wraps" ><div class="mcb-wrap-background-overlay"></div><div class="column mcb-column mcb-item-25822ff54 one laptop-one tablet-one mobile-one column_image" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-25822ff54 mcb-item-image-inner"><div class="image_frame image_item scale-with-grid alignnone no_border mfn-coverimg" role="link" aria-label="Image with links" tabindex="0"><div class="image_wrapper"><a href="https://www.wyatt.com/products/instruments/zetastar.html" class=" mfn-fitimg-wrapper" tabindex="-1" title=""><div class="mask"></div><img width="400" height="400" src="https://www.wyatt.com/wp-content/uploads/2024/08/ZetaStar-400.webp" class="scale-with-grid" alt="" style="" decoding="async" loading="lazy" srcset="https://www.wyatt.com/wp-content/uploads/2024/08/ZetaStar-400.webp 400w, https://www.wyatt.com/wp-content/uploads/2024/08/ZetaStar-400-300x300.webp 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Optional MALS integration.</p></div></div></div><div class="column mcb-column mcb-item-4e77b0ce1 one laptop-one tablet-one mobile-one column_button" style="" ><div class="mcb-column-inner mfn-module-wrapper mcb-column-inner-4e77b0ce1 mcb-item-button-inner"><a class="button button_theme button_size_2" href="https://www.wyatt.com/products/instruments/zetastar.html" title=""><span class="button_label">Learn More</span></a> </div></div></div></div></div></div></div></section></div><section class="section mcb-section the_content no_content"><div class="section_wrapper"><div class="the_content_wrapper "></div></div></section> <section class="section section-page-footer"> <div class="section_wrapper clearfix"> <div class="column one page-pager"> <div class="mcb-column-inner"> </div> </div> </div> </section> </div> </main> </div> </div>  <section class="section before-footer mcb-section mfn-default-section mcb-section-arexmxc3 default-width" style="padding-top:70px;"><div 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