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class="page-actions-menu"> <ul id="p-views" class="page-actions-menu__list"> <li id="language-selector" class="page-actions-menu__list-item"> <a role="button" href="#p-lang" data-mw="interface" data-event-name="menu.languages" title="語言" class="cdx-button cdx-button--size-large cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--icon-only cdx-button--weight-quiet language-selector"> <span class="minerva-icon minerva-icon--language"></span> <span>語言</span> </a> </li> <li id="page-actions-watch" class="page-actions-menu__list-item"> <a role="button" id="ca-watch" href="/w/index.php?title=Special:%E7%94%A8%E6%88%B7%E7%99%BB%E5%BD%95&returnto=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" data-event-name="menu.watch" class="cdx-button cdx-button--size-large cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--icon-only cdx-button--weight-quiet menu__item--page-actions-watch"> <span class="minerva-icon minerva-icon--star"></span> <span>監視</span> </a> </li> <li id="page-actions-edit" class="page-actions-menu__list-item"> <a role="button" id="ca-edit" href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit" data-event-name="menu.edit" data-mw="interface" class="cdx-button cdx-button--size-large cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--icon-only cdx-button--weight-quiet edit-page menu__item--page-actions-edit"> <span class="minerva-icon minerva-icon--edit"></span> <span>編輯</span> </a> </li> </ul> </nav>  <div id="mw-content-subtitle" lang="zh-Hant" dir="ltr"></div> </div> <div id="bodyContent" class="content"> <div id="mw-content-text" class="mw-body-content"><script>function mfTempOpenSection(id){var block=document.getElementById("mf-section-"+id);block.className+=" open-block";block.previousSibling.className+=" open-block";}</script><div class="mw-content-ltr mw-parser-output" lang="zh-Hant" dir="ltr"><section class="mf-section-0" id="mf-section-0"><p>在<a href="/wiki/%E8%AE%A1%E7%AE%97%E7%94%9F%E7%89%A9%E5%AD%A6" title="計算生物學">計算生物學</a>中，N50和L50是Contigs(重疊群)或腳手架長度的統計值。N50的計算方式類似於平均值或中位數，但是對長Contigs的權重更大。這種方法廣泛運用於<a href="/wiki/%E5%BA%8F%E5%88%97%E7%B5%84%E8%A3%9D" title="序列組裝">序列組裝</a>中，特別是在參考組裝草稿內的Contigs長度中。這種度量常被用來描述基因組裝配(有時還有其他裝配)的「完整性」。但它的本質是告訴你一些關於Contigs長度分布的信息。<sup id="cite_ref-:0_1-0" class="reference"><a href="#cite_note-:0-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup> </p> </section><div class="mw-heading mw-heading2 section-heading" onclick="mfTempOpenSection(1)"><span class="indicator mf-icon mf-icon-expand mf-icon--small"></span><h2 id="定义"><span id=".E5.AE.9A.E4.B9.89"></span>定義</h2><span class="mw-editsection"> <a role="button" href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit&section=1" title="編輯章節：定義" class="cdx-button cdx-button--size-large cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--icon-only cdx-button--weight-quiet "> <span class="minerva-icon minerva-icon--edit"></span> <span>編輯</span> </a> </span> </div><section class="mf-section-1 collapsible-block" id="mf-section-1"> <div class="mw-heading mw-heading3"><h3 id="N50">N50</h3><span class="mw-editsection"> <a role="button" href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit&section=2" title="編輯章節：N50" class="cdx-button cdx-button--size-large cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--icon-only cdx-button--weight-quiet "> <span class="minerva-icon minerva-icon--edit"></span> <span>編輯</span> </a> </span> </div> <p>N50根據連續性定義裝配質量。按照序列長度的順序排列你的集合中的所有Contigs。首先是最長的Contigs，然後是第二長的Contigs，最後是最短的Contigs。然後，從一開始累加所有Contigs的長度，這樣您就可以得到最長的Contigs+第二長的Contigs+第三長的Contigs，以此類推，直到達到占總裝配長度50%的數字，即為N50。N50可以近似的看作分布質量一半的點：比N50長的所有Contigs的鹼基數於比N50短的所有Contigs的鹼基數在數值上是接近的。 <sup id="cite_ref-:0_1-1" class="reference"><a href="#cite_note-:0-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading3"><h3 id="L50">L50</h3><span class="mw-editsection"> <a role="button" href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit&section=3" title="編輯章節：L50" class="cdx-button cdx-button--size-large cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--icon-only cdx-button--weight-quiet "> <span class="minerva-icon minerva-icon--edit"></span> <span>編輯</span> </a> </span> </div> <p>L50定義為，在給定一組Contigs的情況下，長度總和占基因組大小一半的最小Contigs數。 </p> <ol class="references"> <li id="cite_note-:0-1"><span class="mw-cite-backlink">^ <a href="#cite_ref-:0_1-0"><sup><b>1.0</b></sup></a> <a href="#cite_ref-:0_1-1"><sup><b>1.1</b></sup></a></span> <span class="reference-text"><cite class="citation web">Videvall, Elin. <a rel="nofollow" class="external text" href="https://www.molecularecologist.com/2017/03/29/whats-n50/">What’s N50?</a>. The Molecular Ecologist. 2017-03-29 <span class="reference-accessdate"> [<span class="nowrap">2022-09-28</span>]</span>. （原始內容<a rel="nofollow" class="external text" href="https://web.archive.org/web/20221020151603/https://www.molecularecologist.com/2017/03/29/whats-n50/">存檔</a>於2022-10-20） <span style="font-family: sans-serif; cursor: default; color:var(--color-subtle, #54595d); font-size: 0.8em; bottom: 0.1em; font-weight: bold;" title="連接到美國英語網頁">（美國英語）</span>.</cite><span title="ctx_ver=Z39.88-2004&rfr_id=info%3Asid%2Fzh.wikipedia.org%3AN50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&rft.atitle=What%E2%80%99s+N50%3F&rft.aufirst=Elin&rft.aulast=Videvall&rft.date=2017-03-29&rft.genre=unknown&rft.jtitle=The+Molecular+Ecologist&rft_id=https%3A%2F%2Fwww.molecularecologist.com%2F2017%2F03%2F29%2Fwhats-n50%2F&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal" class="Z3988"><span style="display:none;"> </span></span></span> </li> </ol>    </section></div> <noscript><img src="https://login.m.wikimedia.org/wiki/Special:CentralAutoLogin/start?type=1x1&mobile=1" alt="" width="1" height="1" style="border: none; position: absolute;"></noscript> <div class="printfooter" data-nosnippet="" lang="zh-Hant" dir="ltr">取自「<a dir="ltr" href="https://zh.wikipedia.org/w/index.php?title=N50、L50和相关统计学&oldid=75630567">https://zh.wikipedia.org/w/index.php?title=N50、L50和相关统计学&oldid=75630567</a>」</div></div> </div> <div class="post-content" id="page-secondary-actions"> </div> </main> <footer class="mw-footer minerva-footer" role="contentinfo"> <a class="last-modified-bar" href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=history"> <div class="post-content last-modified-bar__content"> <span class="minerva-icon minerva-icon-size-medium minerva-icon--modified-history"></span> <span class="last-modified-bar__text modified-enhancement" data-user-name="InternetArchiveBot" data-user-gender="unknown" data-timestamp="1674330811"> <span>最後編輯於 2023年1月21日 (星期六) 19:53</span> </span> <span class="minerva-icon minerva-icon-size-small minerva-icon--expand"></span> </div> </a> <div class="post-content footer-content"> <div id='mw-data-after-content'> <div class="read-more-container"></div> </div> <div id="p-lang"> <h4>語言</h4> <section> <ul id="p-variants" class="minerva-languages"><li id="ca-varlang-0" class="ca-variants-zh mw-list-item"><a href="/zh/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh" hreflang="zh"><span>不转换</span></a></li><li id="ca-varlang-1" class="ca-variants-zh-Hans mw-list-item"><a href="/zh-hans/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans" hreflang="zh-Hans"><span>简体</span></a></li><li id="ca-varlang-2" class="selected ca-variants-zh-Hant mw-list-item"><a href="/zh-hant/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant" hreflang="zh-Hant"><span>繁體</span></a></li><li id="ca-varlang-3" class="ca-variants-zh-Hans-CN mw-list-item"><a href="/zh-cn/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans-CN" hreflang="zh-Hans-CN"><span>大陆简体</span></a></li><li id="ca-varlang-4" class="ca-variants-zh-Hant-HK mw-list-item"><a href="/zh-hk/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant-HK" hreflang="zh-Hant-HK"><span>香港繁體</span></a></li><li id="ca-varlang-5" class="ca-variants-zh-Hant-MO mw-list-item"><a href="/zh-mo/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant-MO" hreflang="zh-Hant-MO"><span>澳門繁體</span></a></li><li id="ca-varlang-6" class="ca-variants-zh-Hans-MY mw-list-item"><a href="/zh-my/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans-MY" hreflang="zh-Hans-MY"><span>大马简体</span></a></li><li id="ca-varlang-7" class="ca-variants-zh-Hans-SG mw-list-item"><a href="/zh-sg/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans-SG" hreflang="zh-Hans-SG"><span>新加坡简体</span></a></li><li id="ca-varlang-8" class="ca-variants-zh-Hant-TW mw-list-item"><a href="/zh-tw/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant-TW" hreflang="zh-Hant-TW"><span>臺灣正體</span></a></li></ul> <ul class="minerva-languages"><li class="interlanguage-link interwiki-ca mw-list-item"><a href="https://ca.wikipedia.org/wiki/Estad%C3%ADstic_N50" title="Estadístic N50 – 加泰蘭文" lang="ca" hreflang="ca" data-title="Estadístic N50" data-language-autonym="Català" data-language-local-name="加泰蘭文" class="interlanguage-link-target"><span>Català</span></a></li><li class="interlanguage-link interwiki-en mw-list-item"><a href="https://en.wikipedia.org/wiki/N50,_L50,_and_related_statistics" title="N50, L50, and related statistics – 英文" lang="en" hreflang="en" data-title="N50, L50, and related statistics" data-language-autonym="English" data-language-local-name="英文" class="interlanguage-link-target"><span>English</span></a></li><li class="interlanguage-link interwiki-es mw-list-item"><a href="https://es.wikipedia.org/wiki/Estad%C3%ADstico_N50" title="Estadístico N50 – 西班牙文" lang="es" hreflang="es" data-title="Estadístico N50" data-language-autonym="Español" data-language-local-name="西班牙文" class="interlanguage-link-target"><span>Español</span></a></li><li class="interlanguage-link interwiki-ru mw-list-item"><a href="https://ru.wikipedia.org/wiki/%D0%9E%D1%86%D0%B5%D0%BD%D0%BA%D0%B8_%D0%BA%D0%B0%D1%87%D0%B5%D1%81%D1%82%D0%B2%D0%B0_%D1%81%D0%B1%D0%BE%D1%80%D0%BA%D0%B8_%D0%B3%D0%B5%D0%BD%D0%BE%D0%BC%D0%B0" title="Оценки качества сборки генома – 俄文" lang="ru" hreflang="ru" data-title="Оценки качества сборки генома" data-language-autonym="Русский" data-language-local-name="俄文" class="interlanguage-link-target"><span>Русский</span></a></li></ul> </section> </div> <div class="minerva-footer-logo"><img src="/static/images/mobile/copyright/wikipedia-wordmark-zh.svg" alt="維基百科" width="105" height="22" style="width: 6.5625em; 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