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class="vector-dropdown-label-text">个人工具</span> </label> <div class="vector-dropdown-content"> <div id="p-personal" class="vector-menu mw-portlet mw-portlet-personal user-links-collapsible-item" title="用户菜单" > <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="pt-sitesupport" class="user-links-collapsible-item mw-list-item"><a href="https://donate.wikimedia.org/?utm_source=donate&utm_medium=sidebar&utm_campaign=spontaneous&uselang=zh-hans"><span>资助维基百科</span></a></li><li id="pt-createaccount" class="user-links-collapsible-item mw-list-item"><a href="/w/index.php?title=Special:%E5%88%9B%E5%BB%BA%E8%B4%A6%E6%88%B7&returnto=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&returntoquery=variant%3Dzh" title="我们推荐您创建账号并登录，但这不是强制性的"><span class="vector-icon mw-ui-icon-userAdd mw-ui-icon-wikimedia-userAdd"></span> <span>创建账号</span></a></li><li id="pt-login" class="user-links-collapsible-item mw-list-item"><a href="/w/index.php?title=Special:%E7%94%A8%E6%88%B7%E7%99%BB%E5%BD%95&returnto=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&returntoquery=variant%3Dzh" title="建议你登录，尽管并非必须。[o]" accesskey="o"><span class="vector-icon mw-ui-icon-logIn mw-ui-icon-wikimedia-logIn"></span> <span>登录</span></a></li> </ul> </div> </div> <div id="p-user-menu-anon-editor" class="vector-menu mw-portlet mw-portlet-user-menu-anon-editor" > <div class="vector-menu-heading"> 未登录编辑者的页面 <a href="/wiki/Help:%E6%96%B0%E6%89%8B%E5%85%A5%E9%97%A8" aria-label="了解有关编辑的更多信息"><span>了解详情</span></a> </div> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="pt-anoncontribs" class="mw-list-item"><a href="/wiki/Special:%E6%88%91%E7%9A%84%E8%B4%A1%E7%8C%AE" title="来自此IP地址的编辑列表[y]" accesskey="y"><span>贡献</span></a></li><li id="pt-anontalk" class="mw-list-item"><a href="/wiki/Special:%E6%88%91%E7%9A%84%E8%AE%A8%E8%AE%BA%E9%A1%B5" title="对于来自此IP地址编辑的讨论[n]" accesskey="n"><span>讨论</span></a></li> </ul> </div> </div> </div> </div> </nav> </div> </header> </div> <div class="mw-page-container"> <div class="mw-page-container-inner"> <div class="vector-sitenotice-container"> <div id="siteNotice"></div> </div> <div class="vector-column-start"> <div class="vector-main-menu-container"> <div id="mw-navigation"> <nav id="mw-panel" class="vector-main-menu-landmark" aria-label="站点"> <div id="vector-main-menu-pinned-container" class="vector-pinned-container"> </div> </nav> </div> </div> <div class="vector-sticky-pinned-container"> <nav id="mw-panel-toc" aria-label="目录" data-event-name="ui.sidebar-toc" class="mw-table-of-contents-container vector-toc-landmark"> <div id="vector-toc-pinned-container" class="vector-pinned-container"> <div id="vector-toc" class="vector-toc vector-pinnable-element"> <div class="vector-pinnable-header vector-toc-pinnable-header vector-pinnable-header-pinned" data-feature-name="toc-pinned" data-pinnable-element-id="vector-toc" > <h2 class="vector-pinnable-header-label">目录</h2> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-pin-button" data-event-name="pinnable-header.vector-toc.pin">移至侧栏</button> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-unpin-button" data-event-name="pinnable-header.vector-toc.unpin">隐藏</button> </div> <ul class="vector-toc-contents" id="mw-panel-toc-list"> <li id="toc-mw-content-text" class="vector-toc-list-item vector-toc-level-1"> <a href="#" class="vector-toc-link"> <div class="vector-toc-text">序言</div> </a> </li> <li id="toc-定义" class="vector-toc-list-item vector-toc-level-1 vector-toc-list-item-expanded"> <a class="vector-toc-link" href="#定义"> <div class="vector-toc-text"> <span class="vector-toc-numb">1</span> <span>定义</span> </div> </a> <button aria-controls="toc-定义-sublist" class="cdx-button cdx-button--weight-quiet cdx-button--icon-only vector-toc-toggle"> <span class="vector-icon mw-ui-icon-wikimedia-expand"></span> <span>开关定义子章节</span> </button> <ul id="toc-定义-sublist" class="vector-toc-list"> <li id="toc-N50" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#N50"> <div class="vector-toc-text"> <span class="vector-toc-numb">1.1</span> <span>N50</span> </div> </a> <ul id="toc-N50-sublist" class="vector-toc-list"> </ul> </li> <li id="toc-L50" class="vector-toc-list-item vector-toc-level-2"> <a class="vector-toc-link" href="#L50"> <div class="vector-toc-text"> <span class="vector-toc-numb">1.2</span> <span>L50</span> </div> </a> <ul id="toc-L50-sublist" class="vector-toc-list"> </ul> </li> </ul> </li> </ul> </div> </div> </nav> </div> </div> <div class="mw-content-container"> <main id="content" class="mw-body"> <header class="mw-body-header vector-page-titlebar"> <nav aria-label="目录" class="vector-toc-landmark"> <div id="vector-page-titlebar-toc" class="vector-dropdown vector-page-titlebar-toc vector-button-flush-left" > <input type="checkbox" id="vector-page-titlebar-toc-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-page-titlebar-toc" class="vector-dropdown-checkbox " aria-label="开关目录" > <label id="vector-page-titlebar-toc-label" for="vector-page-titlebar-toc-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--icon-only " aria-hidden="true" ><span class="vector-icon mw-ui-icon-listBullet mw-ui-icon-wikimedia-listBullet"></span> <span class="vector-dropdown-label-text">开关目录</span> </label> <div class="vector-dropdown-content"> <div id="vector-page-titlebar-toc-unpinned-container" class="vector-unpinned-container"> </div> </div> </div> </nav> <h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">N50、L50和相关统计学</span></h1> <div id="p-lang-btn" class="vector-dropdown mw-portlet mw-portlet-lang" > <input type="checkbox" id="p-lang-btn-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-p-lang-btn" class="vector-dropdown-checkbox mw-interlanguage-selector" aria-label="前往另一种语言写成的文章。4种语言可用" > <label id="p-lang-btn-label" for="p-lang-btn-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--action-progressive mw-portlet-lang-heading-4" aria-hidden="true" ><span class="vector-icon mw-ui-icon-language-progressive mw-ui-icon-wikimedia-language-progressive"></span> <span class="vector-dropdown-label-text">4种语言</span> </label> <div class="vector-dropdown-content"> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li class="interlanguage-link interwiki-ca mw-list-item"><a href="https://ca.wikipedia.org/wiki/Estad%C3%ADstic_N50" title="Estadístic N50 – 加泰罗尼亚语" lang="ca" hreflang="ca" data-title="Estadístic N50" data-language-autonym="Català" data-language-local-name="加泰罗尼亚语" class="interlanguage-link-target"><span>Català</span></a></li><li class="interlanguage-link interwiki-en mw-list-item"><a href="https://en.wikipedia.org/wiki/N50,_L50,_and_related_statistics" title="N50, L50, and related statistics – 英语" lang="en" hreflang="en" data-title="N50, L50, and related statistics" data-language-autonym="English" data-language-local-name="英语" class="interlanguage-link-target"><span>English</span></a></li><li class="interlanguage-link interwiki-es mw-list-item"><a href="https://es.wikipedia.org/wiki/Estad%C3%ADstico_N50" title="Estadístico N50 – 西班牙语" lang="es" hreflang="es" data-title="Estadístico N50" data-language-autonym="Español" data-language-local-name="西班牙语" class="interlanguage-link-target"><span>Español</span></a></li><li class="interlanguage-link interwiki-ru mw-list-item"><a href="https://ru.wikipedia.org/wiki/%D0%9E%D1%86%D0%B5%D0%BD%D0%BA%D0%B8_%D0%BA%D0%B0%D1%87%D0%B5%D1%81%D1%82%D0%B2%D0%B0_%D1%81%D0%B1%D0%BE%D1%80%D0%BA%D0%B8_%D0%B3%D0%B5%D0%BD%D0%BE%D0%BC%D0%B0" title="Оценки качества сборки генома – 俄语" lang="ru" hreflang="ru" data-title="Оценки качества сборки генома" data-language-autonym="Русский" data-language-local-name="俄语" class="interlanguage-link-target"><span>Русский</span></a></li> </ul> <div class="after-portlet after-portlet-lang"><span class="wb-langlinks-edit wb-langlinks-link"><a href="https://www.wikidata.org/wiki/Special:EntityPage/Q8842998#sitelinks-wikipedia" title="编辑跨语言链接" class="wbc-editpage">编辑链接</a></span></div> </div> </div> </div> </header> <div class="vector-page-toolbar"> <div class="vector-page-toolbar-container"> <div id="left-navigation"> <nav aria-label="命名空间"> <div id="p-associated-pages" class="vector-menu vector-menu-tabs mw-portlet mw-portlet-associated-pages" > <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="ca-nstab-main" class="selected vector-tab-noicon mw-list-item"><a href="/wiki/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" title="浏览条目正文[c]" accesskey="c"><span>条目</span></a></li><li id="ca-talk" class="new vector-tab-noicon mw-list-item"><a href="/w/index.php?title=Talk:N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit&redlink=1" rel="discussion" class="new" title="关于此页面的讨论（页面不存在）[t]" accesskey="t"><span>讨论</span></a></li> </ul> </div> </div> <div id="vector-variants-dropdown" class="vector-dropdown " > <input type="checkbox" id="vector-variants-dropdown-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-variants-dropdown" class="vector-dropdown-checkbox " aria-label="更改语言变体" > <label id="vector-variants-dropdown-label" for="vector-variants-dropdown-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet" aria-hidden="true" ><span class="vector-dropdown-label-text">不转换</span> </label> <div class="vector-dropdown-content"> <div id="p-variants" class="vector-menu mw-portlet mw-portlet-variants" > <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="ca-varlang-0" class="selected ca-variants-zh mw-list-item"><a href="/zh/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh" hreflang="zh"><span>不转换</span></a></li><li id="ca-varlang-1" class="ca-variants-zh-Hans mw-list-item"><a href="/zh-hans/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans" hreflang="zh-Hans"><span>简体</span></a></li><li id="ca-varlang-2" class="ca-variants-zh-Hant mw-list-item"><a href="/zh-hant/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant" hreflang="zh-Hant"><span>繁體</span></a></li><li id="ca-varlang-3" class="ca-variants-zh-Hans-CN mw-list-item"><a href="/zh-cn/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans-CN" hreflang="zh-Hans-CN"><span>大陆简体</span></a></li><li id="ca-varlang-4" class="ca-variants-zh-Hant-HK mw-list-item"><a href="/zh-hk/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant-HK" hreflang="zh-Hant-HK"><span>香港繁體</span></a></li><li id="ca-varlang-5" class="ca-variants-zh-Hant-MO mw-list-item"><a href="/zh-mo/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant-MO" hreflang="zh-Hant-MO"><span>澳門繁體</span></a></li><li id="ca-varlang-6" class="ca-variants-zh-Hans-MY mw-list-item"><a href="/zh-my/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans-MY" hreflang="zh-Hans-MY"><span>大马简体</span></a></li><li id="ca-varlang-7" class="ca-variants-zh-Hans-SG mw-list-item"><a href="/zh-sg/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hans-SG" hreflang="zh-Hans-SG"><span>新加坡简体</span></a></li><li id="ca-varlang-8" class="ca-variants-zh-Hant-TW mw-list-item"><a href="/zh-tw/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6" lang="zh-Hant-TW" hreflang="zh-Hant-TW"><span>臺灣正體</span></a></li> </ul> </div> </div> </div> </div> </nav> </div> <div id="right-navigation" class="vector-collapsible"> <nav aria-label="查看"> <div id="p-views" class="vector-menu vector-menu-tabs mw-portlet mw-portlet-views" > <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li id="ca-view" class="selected vector-tab-noicon mw-list-item"><a href="/wiki/N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6"><span>阅读</span></a></li><li id="ca-edit" class="vector-tab-noicon mw-list-item"><a href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit" title="编辑该页面[e]" accesskey="e"><span>编辑</span></a></li><li id="ca-history" class="vector-tab-noicon mw-list-item"><a href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=history" title="本页面的早前版本。[h]" accesskey="h"><span>查看历史</span></a></li> </ul> </div> </div> </nav> <nav class="vector-page-tools-landmark" aria-label="页面工具"> <div id="vector-page-tools-dropdown" class="vector-dropdown vector-page-tools-dropdown" > <input type="checkbox" id="vector-page-tools-dropdown-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-vector-page-tools-dropdown" class="vector-dropdown-checkbox " aria-label="工具" > <label id="vector-page-tools-dropdown-label" for="vector-page-tools-dropdown-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet" aria-hidden="true" ><span class="vector-dropdown-label-text">工具</span> </label> <div class="vector-dropdown-content"> <div id="vector-page-tools-unpinned-container" class="vector-unpinned-container"> <div 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data-feature-name="appearance-pinned" data-pinnable-element-id="vector-appearance" data-pinned-container-id="vector-appearance-pinned-container" data-unpinned-container-id="vector-appearance-unpinned-container" > <div class="vector-pinnable-header-label">外观</div> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-pin-button" data-event-name="pinnable-header.vector-appearance.pin">移至侧栏</button> <button class="vector-pinnable-header-toggle-button vector-pinnable-header-unpin-button" data-event-name="pinnable-header.vector-appearance.unpin">隐藏</button> </div> </div> </div> </nav> </div> </div> <div id="bodyContent" class="vector-body" aria-labelledby="firstHeading" data-mw-ve-target-container> <div class="vector-body-before-content"> <div class="mw-indicators"> </div> <div id="siteSub" class="noprint">维基百科，自由的百科全书</div> </div> <div id="contentSub"><div id="mw-content-subtitle"></div></div> <div id="mw-content-text" class="mw-body-content"><div class="mw-content-ltr mw-parser-output" lang="zh" dir="ltr"><p>在<a href="/wiki/%E8%AE%A1%E7%AE%97%E7%94%9F%E7%89%A9%E5%AD%A6" title="计算生物学">计算生物学</a>中，N50和L50是Contigs(重叠群)或脚手架长度的统计值。N50的计算方式类似于平均值或中位数，但是对长Contigs的权重更大。这种方法广泛运用于<a href="/wiki/%E5%BA%8F%E5%88%97%E7%B5%84%E8%A3%9D" title="序列組裝">序列组装</a>中，特别是在参考组装草稿内的Contigs长度中。这种度量常被用来描述基因组装配(有时还有其他装配)的“完整性”。但它的本质是告诉你一些关于Contigs长度分布的信息。<sup id="cite_ref-:0_1-0" class="reference"><a href="#cite_note-:0-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading2"><h2 id="定义"><span id=".E5.AE.9A.E4.B9.89"></span>定义</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit&section=1" title="编辑章节：定义"><span>编辑</span></a><span class="mw-editsection-bracket">]</span></span></div> <div class="mw-heading mw-heading3"><h3 id="N50">N50</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit&section=2" title="编辑章节：N50"><span>编辑</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>N50根据连续性定义装配质量。按照序列长度的顺序排列你的集合中的所有Contigs。首先是最长的Contigs，然后是第二长的Contigs，最后是最短的Contigs。然后，从一开始累加所有Contigs的长度，这样您就可以得到最长的Contigs+第二长的Contigs+第三长的Contigs，以此类推，直到达到占总装配长度50%的数字，即为N50。N50可以近似的看作分布质量一半的点：比N50长的所有Contigs的碱基数于比N50短的所有Contigs的碱基数在数值上是接近的。 <sup id="cite_ref-:0_1-1" class="reference"><a href="#cite_note-:0-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading3"><h3 id="L50">L50</h3><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=N50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&action=edit&section=3" title="编辑章节：L50"><span>编辑</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>L50定义为，在给定一组Contigs的情况下，长度总和占基因组大小一半的最小Contigs数。 </p> <ol class="references"> <li id="cite_note-:0-1"><span class="mw-cite-backlink">^ <a href="#cite_ref-:0_1-0"><sup><b>1.0</b></sup></a> <a href="#cite_ref-:0_1-1"><sup><b>1.1</b></sup></a></span> <span class="reference-text"><cite class="citation web">Videvall, Elin. <a rel="nofollow" class="external text" href="https://www.molecularecologist.com/2017/03/29/whats-n50/">What’s N50?</a>. The Molecular Ecologist. 2017-03-29 <span class="reference-accessdate"> [<span class="nowrap">2022-09-28</span>]</span>. （原始内容<a rel="nofollow" class="external text" href="https://web.archive.org/web/20221020151603/https://www.molecularecologist.com/2017/03/29/whats-n50/">存档</a>于2022-10-20） <span style="font-family: sans-serif; cursor: default; color:var(--color-subtle, #54595d); font-size: 0.8em; bottom: 0.1em; font-weight: bold;" title="连接到美国英语网页">（美国英语）</span>.</cite><span title="ctx_ver=Z39.88-2004&rfr_id=info%3Asid%2Fzh.wikipedia.org%3AN50%E3%80%81L50%E5%92%8C%E7%9B%B8%E5%85%B3%E7%BB%9F%E8%AE%A1%E5%AD%A6&rft.atitle=What%E2%80%99s+N50%3F&rft.aufirst=Elin&rft.aulast=Videvall&rft.date=2017-03-29&rft.genre=unknown&rft.jtitle=The+Molecular+Ecologist&rft_id=https%3A%2F%2Fwww.molecularecologist.com%2F2017%2F03%2F29%2Fwhats-n50%2F&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal" class="Z3988"><span style="display:none;"> </span></span></span> </li> </ol>    </div><noscript><img src="https://login.wikimedia.org/wiki/Special:CentralAutoLogin/start?type=1x1" alt="" width="1" height="1" style="border: none; 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