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Immunoprecipitation | Proteintech
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class="form-check-input" type="checkbox" name="species-reactivity" value="Chicken" id="species-reactivity-Chicken"> <label class="form-check-label" for="species-reactivity-Chicken"> Chicken </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Zebrafish" id="species-reactivity-Zebrafish"> <label class="form-check-label" for="species-reactivity-Zebrafish"> Zebrafish </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Hamster" id="species-reactivity-Hamster"> <label class="form-check-label" for="species-reactivity-Hamster"> Hamster </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Monkey" id="species-reactivity-Monkey"> <label class="form-check-label" 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class="breadcrumbs"> <ul class="list-inline m-0 ptg-black"> <li class="list-inline-item"><a href="/">Home</a></li> <li class="list-inline-item">Immunoprecipitation</li> </ul> </div> </div> </div> <span class="d-inline-block d-lg-none mt-5"></span> </div> </div> </section> <section class="bg-ptg-white pb-0"> <div class="container-fluid" style="background-size: cover; opacity: .9; background-image: url('https://www.ptglab.com/media/1905/cell-immuno-tile-header.jpg');"> <span><br><br></span> <div class="container-xl"> <div class="col"> <h1 class="ptg-white mt-5">Immunoprecipitation</h1> <p class="ptg-white"> Immunoprecipitation (IP) is a precipitation technique that purifies and enriches a protein of interest, allowing the identification of protein-protein interactions in proteomics workflows. IP is an important technique used to investigate the presence, relative abundance, size, upregulation or downregulation, stability, post-translational modifications (PTMs), and interactions between proteins. </p> <span class="d-none d-lg-inline-block" style="height: 150px;"></span> <span class="d-inline-block d-lg-none" style="height: 100px;"></span> </div> </div> </div> </section> <section class="bg-ptg-white"> <div class="container-xl"> <div class="row justify-content-center"> <div class="col-12"> <div class="row"> <div class="col-12 col-lg-8"> <div class="row"> <div class="col-12 col-md-8 col-lg-6"> <div class="card card__signpost-category"> <div class="card-body bg-ptg-grey-6"> <h4 class="card-title">Antibodies for endogenous targets</h4> <p class="card-text ptg-grey-4"> Explore polyclonal or monoclonal primary antibodies for the IP of untagged, endogenous proteins of interest. </p> <a href="/results?q=immunoprecipitation" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="col-12 col-md-8 col-lg-6"> <div class="card card__signpost-category"> <div class="card-body bg-ptg-grey-6"> <h4 class="card-title">Nanobodies for proteins and peptide tags</h4> <p class="card-text ptg-grey-4">Explore ChromoTek’s Nano-Traps for the IP of proteins carrying protein tags like GFP, mCherry or GST or carrying epitope tags like Spot-tag®, Myc-tag or V5-tag. </p> <a href="/products/chromotek-nanobody-based-reagents/nano-traps/" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="col-12 col-md-8 col-lg-6"> <div class="card card__signpost-category"> <div class="card-body bg-ptg-grey-6"> <h4 class="card-title">Western blot after IP</h4> <p class="card-text ptg-grey-4">Explore WB products for the detection of the precipitated protein of interest and its interaction partners: </p> <ul class="card-text ptg-grey-4"> <li>Over 13,000 polyclonal and monoclonal primary antibodies against the protein of interest </li> <li>Tag antibodies against a protein or peptide tag </li> <li>Secondary antibodies </li> <li>WB accessories [SignalBright, Protein ladders] </li> </ul> <a href="/applications/western-blot/" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="col-12 col-md-8 col-lg-6"> <div class="card card__signpost-category"> <div class="card-body bg-ptg-grey-6"> <h4 class="card-title">Isotype control antibodies</h4> <p class="card-text ptg-grey-4">Explore isotype control antibodies to find the right negative control antibody for your IP antibody to ensure highly specific results.</p> <a href="/results?q=isotype+controls" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> </div> <div class="row pt-5"> <div class="col"> <h4>Watch why GFP-Trap gives the best results for immunoprecipitation (IP)</h4> <p>Low background, no extra bands & high specificity will improve your pulldown assay significantly. Alpaca Alice shows how it works. Visit <a href="/nano-trap-free-sample/" class="text-underline ptg-chromotek fw-bold">www.ptglab.com/nano-trap-free-sample/</a> to order a free test sample.</p> <div class="row justify-content-center"> <div class="col"> <script src="https://fast.wistia.com/embed/medias/7fifdikeet.jsonp" async></script> <script src="https://fast.wistia.com/assets/external/E-v1.js" async></script><div class="wistia_responsive_padding" style="padding:56.25% 0 0 0;position:relative;"><div class="wistia_responsive_wrapper" style="height:100%;left:0;position:absolute;top:0;width:100%;"><div class="wistia_embed wistia_async_7fifdikeet videoFoam=true" style="height:100%;position:relative;width:100%"><div class="wistia_swatch" style="height:100%;left:0;opacity:0;overflow:hidden;position:absolute;top:0;transition:opacity 200ms;width:100%;"><img src="https://fast.wistia.com/embed/medias/7fifdikeet/swatch" style="filter:blur(5px);height:100%;object-fit:contain;width:100%;" alt="" aria-hidden="true" onload="this.parentNode.style.opacity=1;" /></div></div></div></div> </div> </div> </div> </div> </div> <div class="col-12 col-lg-4 offset-lg-0 d-none d-lg-block ps-lg-5"> <div class="row justify-content-center"> <img src="https://www.ptglab.com/media/4220/ip-1.jpg" alt="immunoprecipitation workflow diagram" class="pb-5"> <div class="row pt-3"> <div class="col"> <h5>More videos</h5> <ul class="subtitle"> <li><a href="/videos/immunoprecipitation/save-hours-on-your-immunoprecipitation-experiments/">Immunoprecipitation experiments in less than 2 hours</a></li> <li><a href="/videos/immunoprecipitation/ip-troubleshooting/">IP Troubleshooting</a></li> <li><a href="/videos/immunoprecipitation/co-ip-troubleshooting/">Co-IP troubleshooting</a></li> <li><a href="/videos/immunoprecipitation/enhance-your-ip-and-if-experiments-with-nanobodies-and-antibodies/">Enhance your IP and IF experiments with nanobodies and antibodies</a></li> <li><a href="/videos/immunoprecipitation/immunoprecipitation-workshop/">Immunoprecipitation workshop - All about Immunoprecipitation</a></li> </ul> </div> </div> </div> </div> </div> </div> </div> </div> </section> <section class="carousel-products bg-ptg-white pt-0 pb-0"> <div class="container-xl"> <div class="row"> <div class="col"> <div class="centered-box mb-4"> <h3 class="m-0">Featured Products</h3> </div> </div> </div> <div class="main-carousel" data-flickity='{ "watchCSS": false, "contain": true, "groupCells": true, "pageDots": false, "freeScroll": true, "cellAlign": "left" }'> <div class="carousel-cell"> <div class="card card__product"> <div class="card-header" style="background-image: url('https://www.ptglab.com/media/6685/chromotek-gfp-trap-agarose.png');"> </div> <div class="card-body"> <span class="tag tag-promo">2,863 Publications</span> <h3 class="card-title">GFP-Trap® Agarose</h3> <table class="table table-borderless table-sm"> <tbody> <tr> <td>Specificity</td> <td>GFP, eGFP, CFP, YFP, BFP</td> </tr> <tr> <td>Applications</td> <td>IP, CoIP, ChIP, RIP</td> </tr> </tbody> </table> <a href="/products/GFP-Trap-Agarose-gta.htm" target="_blank" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="carousel-cell"> <div class="card card__product"> <div class="card-header" style="background-image: url('https://www.ptglab.com/media/6687/chromotek-myc-trap-agarose.png');"> </div> <div class="card-body"> <span class="tag tag-promo">77 Publications</span> <h3 class="card-title">Myc-Trap® Agarose</h3> <table class="table table-borderless table-sm"> <tbody> <tr> <td>Specificity</td> <td>Myc-Tag</td> </tr> <tr> <td>Applications</td> <td>IP, CoIP, ChIP, RIP</td> </tr> </tbody> </table> <a href="/products/Myc-Trap-Agarose-yta.htm" target="_blank" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="carousel-cell"> <div class="card card__product"> <div class="card-header" style="background-image: 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table-borderless table-sm"> <tbody> <tr> <td>Reactivity</td> <td>Rabbit, Human, Mouse, Plasmodium Falciparum, Rat</td> </tr> <tr> <td>Applications</td> <td>WB, RIP, IP, IHC, IF, FC, ChIP, ELISA</td> </tr> </tbody> </table> <a href="/products/IgG-control-Antibody-30000-0-AP.htm" target="_blank" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> </div> </div> </section> <section class="homepage-featured-products bg-ptg-white pt-0"> <div class="container-xl"> <div class="row"> <div class="col"> <div class="centered-box mb-4"> <h3 class="m-0">Resources</h3> </div> </div> </div> <div class="row d-flex d-md-none"> <div class="col-12"> <div class="card card__featured-product"> <div class="card-body"> <p class="tag tag-featured-product mb-2">Videos</p> <h5 class="card-title">Immunoprecipitation Videos</h5> <p class="card-text">Short technical tips videos on how to optimize your IP experiments</p> <a href="/videos/immunoprecipitation/" class="visually-hidden 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class="main-carousel" data-flickity='{ "watchCSS": true, "contain": true, "groupCells": true, "pageDots": false, "freeScroll": true, "cellAlign": "left" }'> <!-- watchCSS: true/false controls whether the carousel is shown for mobile --> <div class="carousel-cell"> <div class="card card__featured-product"> <div class="card-header" style="background-image: url('https://www.ptglab.com/media/6590/low-bacground-no-extra-bands-and-high-specificity-in-your-ip-experiment-with-chromotek-gfp-trap.png');"> </div> <div class="card-body"> <p class="tag tag-featured-product mb-2">Videos</p> <h5 class="card-title">Immunoprecipitation Videos</h5> <p class="card-text">Short technical tips videos on how to optimize your IP experiments</p> <a href="/videos/immunoprecipitation/" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="carousel-cell"> <div class="card card__featured-product"> <div class="card-header" style="background-image: url('https://www.ptglab.com/media/3974/ip-header.jpg?');"> </div> <div class="card-body"> <p class="tag tag-featured-product mb-2">Protocols</p> <h5 class="card-title">Immunoprecipitation Guide</h5> <p class="card-text">Protocols and tips for optimizing your IP experiments</p> <a href="/support/immunoprecipitation-protocol/introduction-to-immunoprecipitation/" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="carousel-cell"> <div class="card card__featured-product"> <div class="card-header" style="background-image: url('https://www.ptglab.com/media/2092/wb-loading-image-header2.jpg');"> </div> <div class="card-body"> <p class="tag tag-featured-product mb-2">Blog</p> <h5 class="card-title">Optimize your western blot protocol</h5> <p class="card-text">7 Tips For Optimizing Your Western Blotting Experiments.</p> <a href="/news/blog/7-tips-for-optimizing-your-western-blotting-experiments/" class="visually-hidden stretched-link">Non visible text</a> </div> </div> </div> <div class="col-12 col-lg-3 offset-lg- ps-lg-5 mb-3"> <div class="in-page-nav"> <p class="large">More resources</p> <ul class="list-unstyled"> <li><a href="/news/blog/comparing-bca-bradford-and-uv-protein-quantification-methods-for-scientists/" target="_blank">Comparing BCA, Bradford, and UV quantification methods for scientists </a></li> <li><a href="/news/blog/proteases-for-western-blotting-choosing-the-right-tools-for-the-job/" target="_blank">Proteases for Western Blotting: Choosing the right tools for the job</a></li> <li><a href="/news/blog/lysate-preparation-how-do-i-optimize-my-extraction/" target="_blank">How Do I Optimize My Extraction?</a></li> <li><a href="/news/blog/lysate-preparation-why-is-ripa-buffer-best-for-western-blot/" target="_blank">Why is RIPA Buffer Best for Western Blot?</a></li> <li><a href="/news/blog/tech-tips-in-search-of-low-molecular-weight-proteins/" target="_blank">Western Blot tips for low molecular weight 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