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<!DOCTYPE html> <html > <head> <meta charset="utf-8"> <meta rel="search" type="application/opensearchdescription+xml" href="/open_search.xml" title="Academia.edu"> <meta content="width=device-width, initial-scale=1" name="viewport"> <meta name="google-site-verification" content="bKJMBZA7E43xhDOopFZkssMMkBRjvYERV-NaN4R6mrs"> <meta name="csrf-param" content="authenticity_token" /> <meta name="csrf-token" content="I57FkQl5JDjxSpLZGL2CFqQ7Ea2ka3khkD2AFYYSvq1PQbjibcDoEa29pVLPd3zuX3chhnJO3T1JL1pCjtmB7Q==" /> <meta name="citation_title" content="Metabolic Alteration of Trypanosoma cruzi during Differentiation of Epimastigote to Trypomastigote Forms" /> <meta name="citation_journal_title" content="Pathogens" /> <meta name="citation_author" content="Saulo Cabral Bourguignon" /> <meta name="twitter:card" content="summary" /> <meta name="twitter:url" content="https://www.academia.edu/96454513/Metabolic_Alteration_of_Trypanosoma_cruzi_during_Differentiation_of_Epimastigote_to_Trypomastigote_Forms" /> <meta name="twitter:title" content="Metabolic Alteration of Trypanosoma cruzi during Differentiation of Epimastigote to Trypomastigote Forms" /> <meta name="twitter:description" content="Intracellular parasites such as Trypanosoma cruzi need to acquire valuable carbon sources from the host cell to replicate. 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Here, we investigated the energetic metabolism of T. cruzi during metacyclogenesis through the determination of enzymatic" /> <meta property="article:author" content="https://independent.academia.edu/SCabralBourguignon" /> <meta name="description" content="Intracellular parasites such as Trypanosoma cruzi need to acquire valuable carbon sources from the host cell to replicate. Here, we investigated the energetic metabolism of T. cruzi during metacyclogenesis through the determination of enzymatic" /> <title>(PDF) Metabolic Alteration of Trypanosoma cruzi during Differentiation of Epimastigote to Trypomastigote Forms</title> <link rel="canonical" href="https://www.academia.edu/96454513/Metabolic_Alteration_of_Trypanosoma_cruzi_during_Differentiation_of_Epimastigote_to_Trypomastigote_Forms" /> <script async src="https://www.googletagmanager.com/gtag/js?id=G-5VKX33P2DS"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-5VKX33P2DS', { cookie_domain: 'academia.edu', send_page_view: false, }); gtag('event', 'page_view', { 'controller': "single_work", 'action': "show", 'controller_action': 'single_work#show', 'logged_in': 'false', 'edge': 'unknown', // Send nil if there is no A/B test bucket, in case some records get logged // with missing data - that way we can distinguish between the two cases. // ab_test_bucket should be of the form <ab_test_name>:<bucket> 'ab_test_bucket': null, }) </script> <script> var $controller_name = 'single_work'; var $action_name = "show"; var $rails_env = 'production'; var $app_rev = '68696952412eda9ecbccfe48878af3aa3501b627'; var $domain = 'academia.edu'; var $app_host = "academia.edu"; var $asset_host = "academia-assets.com"; var $start_time = new Date().getTime(); var $recaptcha_key = "6LdxlRMTAAAAADnu_zyLhLg0YF9uACwz78shpjJB"; var $recaptcha_invisible_key = "6Lf3KHUUAAAAACggoMpmGJdQDtiyrjVlvGJ6BbAj"; var $disableClientRecordHit = false; </script> <script> window.require = { config: function() { return function() {} } } </script> <script> window.Aedu = window.Aedu || {}; window.Aedu.hit_data = null; window.Aedu.serverRenderTime = new Date(1736897206000); window.Aedu.timeDifference = new Date().getTime() - 1736897206000; </script> <script type="application/ld+json">{"@context":"https://schema.org","@type":"ScholarlyArticle","abstract":"Intracellular parasites such as Trypanosoma cruzi need to acquire valuable carbon sources from the host cell to replicate. Here, we investigated the energetic metabolism of T. cruzi during metacyclogenesis through the determination of enzymatic activities and quantification by HPLC of glycolytic and Krebs cycle short-chain carboxylic acids. Altered concentrations in pyruvate, acetate, succinate, and glycerate were measured during the growth of epimastigote in the complex medium BHI and their differentiation to trypomastigotes in the chemically defined medium, TAU3AAG. These alterations should represent significant differential metabolic modifications utilized by either form to generate energy. This paper is the first work dealing with the intracellular organic acid concentration measurement in T. cruzi parasites. Although it confirms the previous assumption of the importance of carbohydrate metabolism, it yields an essential improvement in T. cruzi metabolism knowledge.","author":[{"@context":"https://schema.org","@type":"Person","name":"Saulo Cabral Bourguignon"}],"contributor":[],"dateCreated":"2023-02-07","headline":"Metabolic Alteration of Trypanosoma cruzi during Differentiation of Epimastigote to Trypomastigote Forms","image":"https://attachments.academia-assets.com/98348678/thumbnails/1.jpg","inLanguage":"en","keywords":["Biochemistry","Metabolism","Biology","Medicine","Trypanosoma Cruzi","Pathogens","Intracellular"],"publication":"Pathogens","publisher":{"@context":"https://schema.org","@type":"Organization","name":"MDPI AG"},"sourceOrganization":[{"@context":"https://schema.org","@type":"EducationalOrganization","name":null}],"thumbnailUrl":"https://attachments.academia-assets.com/98348678/thumbnails/1.jpg","url":"https://www.academia.edu/96454513/Metabolic_Alteration_of_Trypanosoma_cruzi_during_Differentiation_of_Epimastigote_to_Trypomastigote_Forms"}</script><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/single_work_page/loswp-69556be8c73b29a575811f97c49ddc0244a4f6ac5828c168fca4a6cd6c8f069b.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/body-8d679e925718b5e8e4b18e9a4fab37f7eaa99e43386459376559080ac8f2856a.css" /><link rel="stylesheet" media="all" href="//a.academia-assets.com/assets/design_system/button-3cea6e0ad4715ed965c49bfb15dedfc632787b32ff6d8c3a474182b231146ab7.css" /><link rel="stylesheet" media="all" 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Here, we investigated the energetic metabolism of T. cruzi during metacyclogenesis through the determination of enzymatic activities and quantification by HPLC of glycolytic and Krebs cycle short-chain carboxylic acids. Altered concentrations in pyruvate, acetate, succinate, and glycerate were measured during the growth of epimastigote in the complex medium BHI and their differentiation to trypomastigotes in the chemically defined medium, TAU3AAG. These alterations should represent significant differential metabolic modifications utilized by either form to generate energy. This paper is the first work dealing with the intracellular organic acid concentration measurement in T. cruzi parasites. 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