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However, the expression &amp; purification process of membrane proteins is rather complicated and challenging due to their, hydrophobic nature, sophisticated structure, low natural abundance, unstability, toxicity, etc. After years of efforts and dedication, Creative Biolabs has launched the innovative whole cell antigen presentation technology. We have successfully developed multiple stably transfected cell lines of more than 200 membrane proteins (e.g. GPCR, ion channel, transporter). Notably, we also offer cutting-edge <a href="/anti-membrane-protein-antibody-production-by-dendritic-cell-antigen-presentation.html">anti-membrane protein antibody production by dendritic cell antigen presentation</a> service. By presenting antigens on mammalian cell plasma membranes, membrane proteins of interest can bypass all the drawbacks of <em>in vitro</em> antigen preparation, thus can be well applied in a variety of applications. </p> <p class="ServiceShowPic"> <img src="static/img/Membrane Protein Antibody Production by Whole Cell Immunization-2.jpg" width="313" height="324" alt="Anti-Membrane Protein Antibody Production by Whole Cell Antigen Presentation" /> </p> <p> <strong>Advantages of Whole Cell Antigen Presentation</strong> </p> <p> Compared with membrane protein antigens production <em>in vitro</em>, whole cell antigen presentation shows several appealing advantages. First, it is very difficult to prepare membrane protein antigens from plasma membranes into pure, homogenous and conformationally stable forms. In contrary, whole cell antigen presentation provides an easier alternative to keep membrane protein antigens in their native membrane environment with correct, stable, and functional foldings. No need for tedious purification, reconstitution steps. Moreover, detergents are usually a must to solubilize membrane protein targets from cell membranes, which have potential risks to disturb/impact original protein structure or modifications (especially long-chain detergents that tend to mask extracellular epitopes). In some cases, the functionality/conformation features cannot be restored, resulting in denatured products. While for whole cell presentation, no detergent or solubilization processes are needed. </p> <p> Our technology involves immunization by injection of cultured cells stably over-expressing the antigens that avoid many drawbacks of <em>in vitro</em> antigen production. We have successfully generated many superior anti-membrane protein antibodies <em>via</em> this strategy. </p> <p> <strong>Features of Our Service</strong> </p> <ul class="ullist disc"> <li> Customer-oriented, one-stop package </li> <li> Native and diverse epitopes </li> <li> Ready-to-use stable cell lines overexpressing more than 200 membrane targets </li> <li> All-around antibody discovery platforms </li> <li> Ph.D. scientific teams and conscientious technique support </li> </ul> <br /> <p> In particular, we have successfully incorporated whole cell antigen presentation into following antibody discovery choices: </p> <ul class="ullist disc"> <li> <a href="/Phage-display-technology-for-anti-membrane-protein-antibody-screening-and-production.html">Anti-Membrane Protein Antibody Discovery by Phage Display Technology</a> </li> <li> <a href="/Hybridoma-preparation-and-anti-membrane-protein-mAbs-production-service.html">Anti-Membrane Protein Antibody Discovery Using Hybridoma</a> </li> <li> <a href="/anti-membrane-protein-antibody-discovery-through-premade-antibody-libraries.html">Anti-Membrane Protein Antibody Discovery through Premade Antibody Libraries</a> </li> <li> <a href="/anti-membrane-protein-antibody-discovery-via-native-platform.html">Anti-Membrane Protein Antibody Discovery <em>via</em> Native™ Platform</a> </li> </ul> <br /> <p> <strong>Creative Biolabs</strong> is professional membrane protein immunogen preparation of all kinds. We have successful experience with <a href="/gpcr-preparation-as-immunogen.html">GPCR immunogens</a>, <a href="/ion-channel-preparation-as-immunogen.html">ion channel immunogens</a>, and <a href="/transporter-preparation-as-immunogen.html">transporter immunogens</a>. Please feel free to <a href="/Membrane-Protein-Antibody-Production-by-Whole-Cell-Antigen-Presentation.html#OnlineInquiry">contact us</a> for more information. </p><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.</p> <div class="related-sections"> <b>Related Sections</b> <b>Services</b> <ul class="related-sections-ul"> <li><a href="/anti-membrane-protein-antibody-production-by-dna-immunization.html" >Anti-Membrane Protein Antibody Production by DNA Immunization</a></li> <li><a href="/anti-membrane-protein-antibody-production-using-recombinant-proteins.html" >Anti-Membrane Protein Antibody Production Using Recombinant Proteins</a></li> <li><a href="/anti-membrane-protein-antibody-production-by-dendritic-cell-antigen-presentation.html" >Anti-Membrane Protein Antibody Production by Dendritic Cell Antigen Presentation</a></li> <li><a href="/anti-membrane-protein-antibody-production-by-exosomes.html" >Anti-Membrane Protein Antibody Production by Exosomes</a></li> <li><a href="/Membrane-Protein-Production.html" >Custom Membrane Protein Production Services</a></li> </ul> </div> </div> <div class="col-md-3"> <a name="OnlineInquiry"></a> <div 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