Single B Cell Sorting Platform

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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Platforms</a></li> <li>Single B Cell Sorting Platform</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>Single B Cell Sorting Platform</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">A General Introduction of B Cell Sorting Platform</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Diverse Strategies for Single B Cell Sorting</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Key Features of B Cell Sorting Platform</span> </div> <script>window.addEventListener('load',function(){page_tab_css();page_tab_margin();})</script> <p> To discover unique antibodies in a short-period for our worldwide clients, <strong>Creative Biolabs</strong> currently has pushed out a high-throughput B cell sorting platform, which is able to not only screen a large numbers of B-cells from humans or immunized animals but also identify rare antibodies with the biological activity of interest and favorable manufacturing properties. B cell sorting platform is a state-of-the-art platform in developing native monoclonal antibodies including single B cell screening, cloning and <em>in vitro</em> expression, which makes positive contribution to discovering and developing next-generation antibody therapies in the aim of solving health problems worldwide. </p> <h2 class="h2_tit page_tab_margin" id="tab1"> A General Introduction of B Cell Sorting Platform </h2> <p> Monoclonal antibodies (mAbs) have well established their valuable roles as biomolecules in immunology and have widespread applications in prognosis, diagnosis, and therapeutics in various diseases including cancer, autoimmunity, metabolic and infectious diseases. Here, we describe the novel B cell sorting platform, which is capable of the high-throughput screening and generation of fully human mAbs or natural mAbs of different species in a short period. This platform entails the isolation of B cells from human peripheral blood or immunized animal spleen such as mouse, rabbit or other animals and adopts deep sequencing and single B cell analysis technology to enable the development of potential optimized antibody candidates. The workflow of antibody discovery involves variations on immunization, isolation of B-cells, cloning of single cells, and expression of antibody. We present a technique to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons <em>in vitro</em> expression such as a yeast display platform that is optimized for human Fab surface expression. </p> <p class="ServiceShowPic"> <img src="static/img/B-Cell-Sorting-Platform-1-Creative-Biolabs.png" width="650" height="431" loading="lazy" alt="B-Cell Sorting Platform" /> Fig.1 Workflow for the isolation of monoclonal antibodies by single B lymphocyte sorting. (Hofmann, 2017) </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Diverse Strategies for Single B Cell Sorting </h2> <p> The single plasma cell interrogation platform uses single cell-based screening of entire antibody-secreting B cell populations along with next-generation sequencing to retrieve native antibodies with extremely high <em>in vivo</em> specificity and affinity. During the native antibody development, the isolation and separation of single cells are the main technically challenging tasks. The yield and quality or in other words the integrity and purity of the cells as well as the throughput and the sensitivity of single cell isolation methods should be considered. </p> <p> Today, a large variety of technologies for single-cell separation, isolation, and sorting are already available which mainly include: <a href="/fluorescent-activated-cell-sorting-facs-technology-for-single-cell-isolation.html">FACS (fluorescent activated cell sorting)</a>, <a href="/laser-capture-microdissection-lcm-technology-for-single-cell-isolation.html">LCM (laser-capture microdissection)</a>, <a href="/microengraving-technology-for-single-cell-isolation.html">microengraving</a>, and <a href="/droplet-microfluidics-for-single-cell-isolation.html">droplet microfluidics</a>. </p> <p class="ServiceShowPic"> <img src="static/img/B-cell-sorting-platform-Fig2.png" width="700" height="495" loading="lazy" alt="B-Cell Sorting Platform" />Fig.2 Strategies for single B cell sorting platform. (Gross, 2015) </p> <h2 class="h2_tit page_tab_margin" id="tab3"> Key Features of B Cell Sorting Platform </h2> <ul class="ullist disc"> <li> Ultra-high-throughput screening of B cells for antibody selection </li> <li> Unprecedented single-cell resolution </li> <li> Ideal for important and challenging target cells </li> <li> Available for most species </li> </ul> <br /> <p> <strong>Creative Biolabs</strong> is specialized in antibody discovery and development and harbors extensive experience in antibody generation using our comprehensive platform. We leverage the leading single-B-cell functional antibody discovery technology to develop native antibodies. Based on vast experience and solid technical strength, we currently provide a most comprehensive portfolio of antibodies. We will accelerate the development and potential application of this particular antibody drug discovery approach. </p> <p class="Reference"> <strong>References</strong> </p> <ol class="ollist Reference"> <li> Hofmann, D.; and Lai, J.R. Exploring Human Antimicrobial Antibody Responses on a Single B Cell Level. <em>Clinical and Vaccine Immunology</em>. 2017, 24(5): e00544-16. </li> <li> Gross, A.; <em>et al</em>. Technologies for Single-Cell Isolation. <em>International Journal of Molecular Sciences</em>. 2015, 16, 16897-16919. </li> </ol><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. 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