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The method was optimized with respect to solvent composition of the assay medium, which has a large influence on the course of reaction of tocopherols with DPPH. The rate of reaction of α-tocopherol with DPPH is higher than that of γ-tocopherol in both protic and aprotic solvents. In ethyl acetate, routinely applied for the analysis of antioxidant potential (AOP) of plant oils, reactions of tocopherols with DPPH are slower and concentration of tocopherols in the assay has a large influence on their molar reactivity. In 2-propanol, however, two electrons are exchanged for both α-and γ-tocopherols, independent of their concentration. 2-propanol is not toxic and is fully compatible with polypropylene labware. The chromatographically determined content of tocopherols and their molar reactivity in the DPPH assay reveal that only tocopherols contribute to the AOP of sunflower oil, whereas the contribution of tocopherols to the AOP of linseed oil is 75%. The DPPH assay in 2-propanol can be applied for rapid and cheap estimation of vitamin E content in plant oils where tocopherols are major antioxidants.","publication_date":"2015,,","publication_name":"Molecules","grobid_abstract_attachment_id":"110490816"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [277533442]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div 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data-landing_url="https://www.academia.edu/113563004/Rapid_Estimation_of_Tocopherol_Content_in_Linseed_and_Sunflower_Oils_Reactivity_and_Assay" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="66092762" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/66092762/Antioxidant_effect_of_mono_and_dihydroxyphenols_in_sunflower_oil_with_different_levels_of_naturally_present_tocopherols">Antioxidant effect of mono- and dihydroxyphenols in sunflower oil with different levels of naturally present tocopherols</a><div 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Sirohi</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of Biosciences, 2019</p><p class="ds-related-work--abstract ds2-5-body-sm">Adulteration of Extra Virgin Olive Oil with olive oil and sunflower oil remained major issue in edible oil industry. Herein, rapid, sensitive and precise method for the determination of all type of tocopherols in different vegetable oils includinhg sunflower oil, olive oil and extra virgin olive oil is reported. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) with UV detector was used for tocopherol determination. A simple, quick and sensitive method to estimate the antioxidant quantity is proposed. In this method oils were diluted in methanol and injected directly into column (no saponification procedure). Methanol and water (98:2) mixture was used as mobile phase. Three tocopherols (α, γ and δ) were detected at 292 nm wavelength with UV detector. 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href="https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils">Development of an Advance Method for the Determination of Tocopherols in Edible Oils</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="160629219" href="https://independent.academia.edu/VandaFuss">Vanda Fuss</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Agricultura, 2020</p><p class="ds-related-work--abstract ds2-5-body-sm">We have developed a method for the determination of tocopherols in different edible oils by RP-HPLC, using a FLD detector, a Poroshell column as a stationary phase, and a mixture of acetonitrile, methanol and water (5:4.5:0.5) as mobile phase (flow rate 0.75 ml/min). The structure of the compounds was identical, so it could not be separated by reverse phase liquid chromatography. The obtained peaks show an important overlapping and we have used chemometric tools to get a calibration model, which assumes a linear behavior, i.e., the total signal is the sum of individual signals of components. Recovery percentages were between 90.3% and 95.7%. The method has been successfully applied to edible oils and dietary supplements. No pretreatment of the samples was needed.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Development of an Advance Method for the Determination of Tocopherols in Edible Oils","attachmentId":115676043,"attachmentType":"pdf","work_url":"https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/120568914/Development_of_an_Advance_Method_for_the_Determination_of_Tocopherols_in_Edible_Oils"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="47326000" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/47326000/The_Antioxidant_Activity_of_Tocotrienols_Compared_with_Some_Synthetic_Antioxidant">The Antioxidant Activity of Tocotrienols Compared with Some Synthetic Antioxidant</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="60014373" href="https://independent.academia.edu/HOUSAMHAJHAMDO">HOUSAM HAJ HAMDO</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Pharmacology &amp; Pharmacy, 2014</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"The Antioxidant Activity of Tocotrienols Compared with Some Synthetic Antioxidant","attachmentId":66477404,"attachmentType":"pdf","work_url":"https://www.academia.edu/47326000/The_Antioxidant_Activity_of_Tocotrienols_Compared_with_Some_Synthetic_Antioxidant","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/47326000/The_Antioxidant_Activity_of_Tocotrienols_Compared_with_Some_Synthetic_Antioxidant"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="54031424" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/54031424/Physicochemical_Characteristics_and_HPLC_Determination_of_Alpha_Tocopherol_in_Eighteen_Edible_Vegetable_Oils_Marketed_in_Nigeria">Physicochemical Characteristics and HPLC Determination of Alpha-Tocopherol in Eighteen Edible Vegetable Oils Marketed in Nigeria</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2660325" href="https://oou.academia.edu/olufunmilayoadejumo">olufunmilayo adejumo</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Dhaka University Journal of Pharmaceutical Sciences</p><p class="ds-related-work--abstract ds2-5-body-sm">Eighteen brands of vegetable oils available in the local market were extracted with n-hexane before analysis for alpha-tocopherol by RP-HPLC method. The chromatographic separation occurred isocratically with methanol-water [96:4%v/v] at 0.9 ml/min flow rate. Tocopheryl acetate was the internal standard and alpha-tocopherol was eluted at 7.87 min. Free fatty acids value [FFAVs], peroxide value [PV], iodine value [IV] and saponification values [SV] were determined as quality parameters. Calibration curve was linear [r2 0.9969] and the method was precise with relative standard deviation of 0.35% and mean recovery, 87.39%. Alpha-tocopherol concentration ranged from 0-9.22 mg/100g with the highest in Tropical sunflower oil [9.22 mg/100g] and the lowest [1.16 mg/100g] in Laziz oil. Alpha-tocopherol was not detected in unbranded, local palm oil. The calculated percentage daily value [% DV] of vitamin E ranged from 0- 8.60%. Significant difference [p&lt;0.05] between % DV and recommended di...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Physicochemical Characteristics and HPLC Determination of Alpha-Tocopherol in Eighteen Edible Vegetable Oils Marketed in Nigeria","attachmentId":70591840,"attachmentType":"pdf","work_url":"https://www.academia.edu/54031424/Physicochemical_Characteristics_and_HPLC_Determination_of_Alpha_Tocopherol_in_Eighteen_Edible_Vegetable_Oils_Marketed_in_Nigeria","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/54031424/Physicochemical_Characteristics_and_HPLC_Determination_of_Alpha_Tocopherol_in_Eighteen_Edible_Vegetable_Oils_Marketed_in_Nigeria"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":110490816,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":110490816,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_110490816" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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