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The preferred... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_61714142" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A key and highly regulated enzyme that is required for the biosynthesis of monounsaturated fatty acids is stearoyl-CoA desaturase (SCD), which catalyzes the D9- cis desaturation of a range of fatty acyl-CoA substrates. The preferred substrates are palmitoyl- and stearoyl-CoA, which are converted into palmitoleoyl- and oleoyl-CoA respectively. Oleate is the most abundant monounsaturated fatty acid in dietary fat and is therefore readily available. Studies of mice that have a naturally occurring mutation in the SCD-1 gene isoform as well as a mouse model with a targeted disruption of the SCD gene (SCD-1−/−) have revealed the role of de novo synthesized oleate and thus the physiological importance of SCD-1 expression. SCD-1 deficiency results in reduced body adiposity, increased insulin sensitivity, and resistance to diet-induced obesity. The expression of several genes of lipid oxidation are upregulated, whereas lipid synthesis genes are downregulated. SCD-1 was also found to be a com...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/61714142" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b2bf0cc1dbecdb740665c5b98e7b82cb" rel="nofollow" data-download="{"attachment_id":74681772,"asset_id":61714142,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/74681772/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="65543935" href="https://independent.academia.edu/JNtambi">J. 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The preferred substrates are palmitoyl- and stearoyl-CoA, which are converted into palmitoleoyl- and oleoyl-CoA respectively. Oleate is the most abundant monounsaturated fatty acid in dietary fat and is therefore readily available. Studies of mice that have a naturally occurring mutation in the SCD-1 gene isoform as well as a mouse model with a targeted disruption of the SCD gene (SCD-1−/−) have revealed the role of de novo synthesized oleate and thus the physiological importance of SCD-1 expression. SCD-1 deficiency results in reduced body adiposity, increased insulin sensitivity, and resistance to diet-induced obesity. The expression of several genes of lipid oxidation are upregulated, whereas lipid synthesis genes are downregulated. SCD-1 was also found to be a com...","downloadable_attachments":[{"id":74681772,"asset_id":61714142,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":65543935,"first_name":"J.","last_name":"Ntambi","domain_name":"independent","page_name":"JNtambi","display_name":"J. 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href="https://www.academia.edu/80866673/Xylose_transport_in_yeast_for_lignocellulosic_ethanol_production_Current_status">Xylose transport in yeast for lignocellulosic ethanol production: Current status</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Lignocellulosic ethanol has been considered as an alternative transportation fuel. Utilization of hemicellulosic fraction in lignocelluloses is crucial in economical production of lignocellulosic ethanol. However, this fraction has not... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_80866673" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Lignocellulosic ethanol has been considered as an alternative transportation fuel. Utilization of hemicellulosic fraction in lignocelluloses is crucial in economical production of lignocellulosic ethanol. However, this fraction has not efficiently been utilized by traditional yeast Saccharomyces cerevisiae. Genetically modified S. cerevisiae, which can utilize xylose, has several limitations including low ethanol yield, redox imbalance, and undesired metabolite formation similar to native xylose utilizing yeasts. Besides, xylose uptake is a major issue, where sugar transport system plays an important role. These genetically modified and wild-type yeast strains have further been engineered for improved xylose uptake. Various techniques have been employed to facilitate the xylose transportation in these strains. The present review is focused on the sugar transport machineries, mechanisms of xylose transport, limitations and how to deal with xylose transport for xylose assimilation in ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/80866673" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3dc68ed2dac87f55f338f348950b39db" rel="nofollow" data-download="{"attachment_id":87105741,"asset_id":80866673,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/87105741/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="6412757" href="https://independent.academia.edu/NileshKumarSharma">Nilesh Kumar Sharma</a><script data-card-contents-for-user="6412757" type="text/json">{"id":6412757,"first_name":"Nilesh Kumar","last_name":"Sharma","domain_name":"independent","page_name":"NileshKumarSharma","display_name":"Nilesh Kumar Sharma","profile_url":"https://independent.academia.edu/NileshKumarSharma?f_ri=409525","photo":"https://0.academia-photos.com/6412757/2598353/3018228/s65_nilesh_kumar.sharma.jpg"}</script></span></span></li><li class="js-paper-rank-work_80866673 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="80866673"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 80866673, container: ".js-paper-rank-work_80866673", }); 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Utilization of hemicellulosic fraction in lignocelluloses is crucial in economical production of lignocellulosic ethanol. However, this fraction has not efficiently been utilized by traditional yeast Saccharomyces cerevisiae. Genetically modified S. cerevisiae, which can utilize xylose, has several limitations including low ethanol yield, redox imbalance, and undesired metabolite formation similar to native xylose utilizing yeasts. Besides, xylose uptake is a major issue, where sugar transport system plays an important role. These genetically modified and wild-type yeast strains have further been engineered for improved xylose uptake. Various techniques have been employed to facilitate the xylose transportation in these strains. The present review is focused on the sugar transport machineries, mechanisms of xylose transport, limitations and how to deal with xylose transport for xylose assimilation in ...","downloadable_attachments":[{"id":87105741,"asset_id":80866673,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":6412757,"first_name":"Nilesh Kumar","last_name":"Sharma","domain_name":"independent","page_name":"NileshKumarSharma","display_name":"Nilesh Kumar Sharma","profile_url":"https://independent.academia.edu/NileshKumarSharma?f_ri=409525","photo":"https://0.academia-photos.com/6412757/2598353/3018228/s65_nilesh_kumar.sharma.jpg"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=409525","nofollow":false},{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=409525","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=409525","nofollow":false},{"id":11623,"name":"Metabolic Engineering","url":"https://www.academia.edu/Documents/in/Metabolic_Engineering?f_ri=409525","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=409525"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525"},{"id":49123,"name":"Saccharomyces cerevisiae","url":"https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae?f_ri=409525"},{"id":56291,"name":"Ethanol Fuel","url":"https://www.academia.edu/Documents/in/Ethanol_Fuel?f_ri=409525"},{"id":121705,"name":"Ethanol","url":"https://www.academia.edu/Documents/in/Ethanol?f_ri=409525"},{"id":151659,"name":"Yeast","url":"https://www.academia.edu/Documents/in/Yeast?f_ri=409525"},{"id":173023,"name":"Lignin","url":"https://www.academia.edu/Documents/in/Lignin?f_ri=409525"},{"id":269129,"name":"Fermentation","url":"https://www.academia.edu/Documents/in/Fermentation?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":614751,"name":"Xylose","url":"https://www.academia.edu/Documents/in/Xylose?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_57090211" data-work_id="57090211" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/57090211/Identification_of_the_2_Hydroxyglutarate_and_Isovaleryl_CoA_Dehydrogenases_as_Alternative_Electron_Donors_Linking_Lysine_Catabolism_to_the_Electron_Transport_Chain_of_Arabidopsis_Mitochondria">Identification of the 2-Hydroxyglutarate and Isovaleryl-CoA Dehydrogenases as Alternative Electron Donors Linking Lysine Catabolism to the Electron Transport Chain of Arabidopsis Mitochondria</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The process of dark-induced senescence in plants is relatively poorly understood, but a functional electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) complex, which supports respiration... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_57090211" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The process of dark-induced senescence in plants is relatively poorly understood, but a functional electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) complex, which supports respiration during carbon starvation, has recently been identified. Here, we studied the responses of Arabidopsis thaliana mutants deficient in the expression of isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase to extended darkness and other environmental stresses. Evaluations of the mutant phenotypes following carbon starvation induced by extended darkness identify similarities to those exhibited by mutants of the ETF/ETFQO complex. Metabolic profiling and isotope tracer experimentation revealed that isovaleryl-CoA dehydrogenase is involved in degradation of the branched-chain amino acids, phytol, and Lys, while 2-hydroxyglutarate dehydrogenase is involved exclusively in Lys degradation. These results suggest that isovaleryl-CoA dehydrogenase is ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/57090211" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f41cae42112fcaf00dbe5b30a76d6fed" rel="nofollow" data-download="{"attachment_id":72156398,"asset_id":57090211,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/72156398/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50012476" href="https://independent.academia.edu/KimitsuneIshizaki">Kimitsune Ishizaki</a><script data-card-contents-for-user="50012476" type="text/json">{"id":50012476,"first_name":"Kimitsune","last_name":"Ishizaki","domain_name":"independent","page_name":"KimitsuneIshizaki","display_name":"Kimitsune Ishizaki","profile_url":"https://independent.academia.edu/KimitsuneIshizaki?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_57090211 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="57090211"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 57090211, container: ".js-paper-rank-work_57090211", }); 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Here, we studied the responses of Arabidopsis thaliana mutants deficient in the expression of isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase to extended darkness and other environmental stresses. Evaluations of the mutant phenotypes following carbon starvation induced by extended darkness identify similarities to those exhibited by mutants of the ETF/ETFQO complex. Metabolic profiling and isotope tracer experimentation revealed that isovaleryl-CoA dehydrogenase is involved in degradation of the branched-chain amino acids, phytol, and Lys, while 2-hydroxyglutarate dehydrogenase is involved exclusively in Lys degradation. These results suggest that isovaleryl-CoA dehydrogenase is ...","downloadable_attachments":[{"id":72156398,"asset_id":57090211,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50012476,"first_name":"Kimitsune","last_name":"Ishizaki","domain_name":"independent","page_name":"KimitsuneIshizaki","display_name":"Kimitsune Ishizaki","profile_url":"https://independent.academia.edu/KimitsuneIshizaki?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics?f_ri=409525","nofollow":false},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=409525","nofollow":false},{"id":15719,"name":"Mitochondria","url":"https://www.academia.edu/Documents/in/Mitochondria?f_ri=409525","nofollow":false},{"id":74780,"name":"Mutation","url":"https://www.academia.edu/Documents/in/Mutation?f_ri=409525","nofollow":false},{"id":86644,"name":"Darkness","url":"https://www.academia.edu/Documents/in/Darkness?f_ri=409525"},{"id":186094,"name":"Electron Transport","url":"https://www.academia.edu/Documents/in/Electron_Transport?f_ri=409525"},{"id":202413,"name":"Arabidopsis","url":"https://www.academia.edu/Documents/in/Arabidopsis?f_ri=409525"},{"id":213897,"name":"Phenotype","url":"https://www.academia.edu/Documents/in/Phenotype?f_ri=409525"},{"id":266621,"name":"Electrons","url":"https://www.academia.edu/Documents/in/Electrons?f_ri=409525"},{"id":373650,"name":"Lysine","url":"https://www.academia.edu/Documents/in/Lysine?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":529560,"name":"Gas Chromatography/mass Spectrometry","url":"https://www.academia.edu/Documents/in/Gas_Chromatography_mass_Spectrometry?f_ri=409525"},{"id":715926,"name":"Plant cell","url":"https://www.academia.edu/Documents/in/Plant_cell?f_ri=409525"},{"id":1292327,"name":"Metabolome","url":"https://www.academia.edu/Documents/in/Metabolome?f_ri=409525"},{"id":1341074,"name":"Isotope Labeling","url":"https://www.academia.edu/Documents/in/Isotope_Labeling?f_ri=409525"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=409525"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves?f_ri=409525"},{"id":3364879,"name":"Leucine","url":"https://www.academia.edu/Documents/in/Leucine?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_28839386" data-work_id="28839386" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/28839386/Co_consumption_of_sugars_or_ethanol_and_glucose_in_a_Saccharomyces_cerevisiae_strain_deleted_in_the_HXK2_gene">Co‐consumption of sugars or ethanol and glucose in a Saccharomyces cerevisiae strain deleted in the HXK2 gene</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/28839386" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c0aca370075037740ffaca005627cd77" rel="nofollow" data-download="{"attachment_id":49259310,"asset_id":28839386,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49259310/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="54293143" href="https://uva.academia.edu/JanABerden">Jan A. Berden</a><script data-card-contents-for-user="54293143" type="text/json">{"id":54293143,"first_name":"Jan A.","last_name":"Berden","domain_name":"uva","page_name":"JanABerden","display_name":"Jan A. Berden","profile_url":"https://uva.academia.edu/JanABerden?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_28839386 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="28839386"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 28839386, container: ".js-paper-rank-work_28839386", }); });</script></li><li class="js-percentile-work_28839386 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 28839386; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_28839386"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_28839386 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="28839386"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 28839386; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=28839386]").text(description); $(".js-view-count-work_28839386").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_28839386").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="28839386"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4594" href="https://www.academia.edu/Documents/in/Carbon_Dioxide">Carbon Dioxide</a>, <script data-card-contents-for-ri="4594" type="text/json">{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="14907" href="https://www.academia.edu/Documents/in/Industrial_Biotechnology">Industrial Biotechnology</a>, <script data-card-contents-for-ri="14907" type="text/json">{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="49123" href="https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae">Saccharomyces cerevisiae</a><script data-card-contents-for-ri="49123" type="text/json">{"id":49123,"name":"Saccharomyces cerevisiae","url":"https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=28839386]'), work: {"id":28839386,"title":"Co‐consumption of sugars or ethanol and glucose in a Saccharomyces cerevisiae strain deleted in the HXK2 gene","created_at":"2016-10-01T00:21:17.489-07:00","url":"https://www.academia.edu/28839386/Co_consumption_of_sugars_or_ethanol_and_glucose_in_a_Saccharomyces_cerevisiae_strain_deleted_in_the_HXK2_gene?f_ri=409525","dom_id":"work_28839386","summary":null,"downloadable_attachments":[{"id":49259310,"asset_id":28839386,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":54293143,"first_name":"Jan A.","last_name":"Berden","domain_name":"uva","page_name":"JanABerden","display_name":"Jan A. Berden","profile_url":"https://uva.academia.edu/JanABerden?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false},{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide?f_ri=409525","nofollow":false},{"id":14907,"name":"Industrial Biotechnology","url":"https://www.academia.edu/Documents/in/Industrial_Biotechnology?f_ri=409525","nofollow":false},{"id":49123,"name":"Saccharomyces cerevisiae","url":"https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae?f_ri=409525","nofollow":false},{"id":71289,"name":"Glucose","url":"https://www.academia.edu/Documents/in/Glucose?f_ri=409525"},{"id":121705,"name":"Ethanol","url":"https://www.academia.edu/Documents/in/Ethanol?f_ri=409525"},{"id":151659,"name":"Yeast","url":"https://www.academia.edu/Documents/in/Yeast?f_ri=409525"},{"id":162196,"name":"Sucrose","url":"https://www.academia.edu/Documents/in/Sucrose?f_ri=409525"},{"id":373417,"name":"Quaternary Science Reviews","url":"https://www.academia.edu/Documents/in/Quaternary_Science_Reviews?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":957359,"name":"Culture Media","url":"https://www.academia.edu/Documents/in/Culture_Media?f_ri=409525"},{"id":1035619,"name":"Galactose Binding","url":"https://www.academia.edu/Documents/in/Galactose_Binding?f_ri=409525"},{"id":1193624,"name":"Oxygen Consumption","url":"https://www.academia.edu/Documents/in/Oxygen_Consumption?f_ri=409525"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_16456139" data-work_id="16456139" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/16456139/FAO_WHO_Scientific_Update_on_carbohydrates_in_human_nutrition_conclusions">FAO/WHO Scientific Update on carbohydrates in human nutrition: conclusions</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/16456139" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1d7d4145b9101fd7a4c727378d3c6eac" rel="nofollow" data-download="{"attachment_id":42473933,"asset_id":16456139,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42473933/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35641127" href="https://independent.academia.edu/BernardVenn">Bernard Venn</a><script data-card-contents-for-user="35641127" type="text/json">{"id":35641127,"first_name":"Bernard","last_name":"Venn","domain_name":"independent","page_name":"BernardVenn","display_name":"Bernard Venn","profile_url":"https://independent.academia.edu/BernardVenn?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_16456139 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="16456139"><i class="u-m1x fa 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href="https://www.academia.edu/Documents/in/Nutrition_and_Dietetics">Nutrition and Dietetics</a>, <script data-card-contents-for-ri="591" type="text/json">{"id":591,"name":"Nutrition and Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="3851" href="https://www.academia.edu/Documents/in/Obesity">Obesity</a>, <script data-card-contents-for-ri="3851" type="text/json">{"id":3851,"name":"Obesity","url":"https://www.academia.edu/Documents/in/Obesity?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6617" href="https://www.academia.edu/Documents/in/United_Nations">United Nations</a>, <script data-card-contents-for-ri="6617" type="text/json">{"id":6617,"name":"United Nations","url":"https://www.academia.edu/Documents/in/United_Nations?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="35783" href="https://www.academia.edu/Documents/in/Human_Nutrition">Human Nutrition</a><script data-card-contents-for-ri="35783" type="text/json">{"id":35783,"name":"Human Nutrition","url":"https://www.academia.edu/Documents/in/Human_Nutrition?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=16456139]'), work: {"id":16456139,"title":"FAO/WHO Scientific Update on carbohydrates in human nutrition: conclusions","created_at":"2015-10-04T13:37:43.926-07:00","url":"https://www.academia.edu/16456139/FAO_WHO_Scientific_Update_on_carbohydrates_in_human_nutrition_conclusions?f_ri=409525","dom_id":"work_16456139","summary":null,"downloadable_attachments":[{"id":42473933,"asset_id":16456139,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35641127,"first_name":"Bernard","last_name":"Venn","domain_name":"independent","page_name":"BernardVenn","display_name":"Bernard Venn","profile_url":"https://independent.academia.edu/BernardVenn?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":591,"name":"Nutrition and Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=409525","nofollow":false},{"id":3851,"name":"Obesity","url":"https://www.academia.edu/Documents/in/Obesity?f_ri=409525","nofollow":false},{"id":6617,"name":"United Nations","url":"https://www.academia.edu/Documents/in/United_Nations?f_ri=409525","nofollow":false},{"id":35783,"name":"Human Nutrition","url":"https://www.academia.edu/Documents/in/Human_Nutrition?f_ri=409525","nofollow":false},{"id":97269,"name":"Chronic Disease","url":"https://www.academia.edu/Documents/in/Chronic_Disease?f_ri=409525"},{"id":179865,"name":"Glycemic Index","url":"https://www.academia.edu/Documents/in/Glycemic_Index?f_ri=409525"},{"id":192721,"name":"Risk factors","url":"https://www.academia.edu/Documents/in/Risk_factors?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":469018,"name":"Neoplasms","url":"https://www.academia.edu/Documents/in/Neoplasms?f_ri=409525"},{"id":521881,"name":"World Health Organization","url":"https://www.academia.edu/Documents/in/World_Health_Organization?f_ri=409525"},{"id":546429,"name":"Dietary Carbohydrates","url":"https://www.academia.edu/Documents/in/Dietary_Carbohydrates?f_ri=409525"},{"id":559242,"name":"Cardiovascular Diseases","url":"https://www.academia.edu/Documents/in/Cardiovascular_Diseases?f_ri=409525"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=409525"},{"id":609249,"name":"CARBOHYDRATES","url":"https://www.academia.edu/Documents/in/CARBOHYDRATES?f_ri=409525"},{"id":620049,"name":"Risk Factors","url":"https://www.academia.edu/Documents/in/Risk_Factors-1?f_ri=409525"},{"id":915951,"name":"Type 2 Diabetes Mellitus","url":"https://www.academia.edu/Documents/in/Type_2_Diabetes_Mellitus?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19111252 coauthored" data-work_id="19111252" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19111252/The_effect_of_phosphate_deficiency_on_carbohydrate_metabolism_in_bean_roots">The effect of phosphate deficiency on carbohydrate metabolism in bean roots</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19111252" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c0a5a31db2ebb0de013a085e39f25731" rel="nofollow" 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u-positionRelative">3</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="5541" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="409525" href="https://www.academia.edu/Documents/in/Carbohydrate_metabolism">Carbohydrate metabolism</a>, <script data-card-contents-for-ri="409525" type="text/json">{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1681026" href="https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology">Biochemistry and cell biology</a><script data-card-contents-for-ri="1681026" type="text/json">{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19111252]'), work: {"id":19111252,"title":"The effect of phosphate deficiency on carbohydrate metabolism in bean roots","created_at":"2015-11-27T12:30:42.162-08:00","url":"https://www.academia.edu/19111252/The_effect_of_phosphate_deficiency_on_carbohydrate_metabolism_in_bean_roots?f_ri=409525","dom_id":"work_19111252","summary":null,"downloadable_attachments":[{"id":40437761,"asset_id":19111252,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39295252,"first_name":"Douglas","last_name":"Randall","domain_name":"independent","page_name":"DouglasRandall1","display_name":"Douglas 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data-work_id="62500167" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62500167/Carbohydrate_terminology_and_classification">Carbohydrate terminology and classification</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62500167" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8b35692649b8d92a4f189212c5948a25" rel="nofollow" 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window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_62500167"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_62500167 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="62500167"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 62500167; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=62500167]").text(description); $(".js-view-count-work_62500167").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_62500167").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="62500167"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="532" href="https://www.academia.edu/Documents/in/Physical_Chemistry">Physical Chemistry</a>, <script data-card-contents-for-ri="532" type="text/json">{"id":532,"name":"Physical Chemistry","url":"https://www.academia.edu/Documents/in/Physical_Chemistry?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="591" href="https://www.academia.edu/Documents/in/Nutrition_and_Dietetics">Nutrition and Dietetics</a>, <script data-card-contents-for-ri="591" type="text/json">{"id":591,"name":"Nutrition and Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16061" href="https://www.academia.edu/Documents/in/Polysaccharides">Polysaccharides</a>, <script data-card-contents-for-ri="16061" type="text/json">{"id":16061,"name":"Polysaccharides","url":"https://www.academia.edu/Documents/in/Polysaccharides?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16288" href="https://www.academia.edu/Documents/in/Public_Health">Public Health</a><script data-card-contents-for-ri="16288" type="text/json">{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=62500167]'), work: {"id":62500167,"title":"Carbohydrate terminology and 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Dietetics","url":"https://www.academia.edu/Documents/in/Nutrition_and_Dietetics?f_ri=409525","nofollow":false},{"id":16061,"name":"Polysaccharides","url":"https://www.academia.edu/Documents/in/Polysaccharides?f_ri=409525","nofollow":false},{"id":16288,"name":"Public Health","url":"https://www.academia.edu/Documents/in/Public_Health?f_ri=409525","nofollow":false},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":546429,"name":"Dietary Carbohydrates","url":"https://www.academia.edu/Documents/in/Dietary_Carbohydrates?f_ri=409525"},{"id":573653,"name":"Food Sciences","url":"https://www.academia.edu/Documents/in/Food_Sciences?f_ri=409525"},{"id":598869,"name":"Water soluble polymers","url":"https://www.academia.edu/Documents/in/Water_soluble_polymers?f_ri=409525"},{"id":837135,"name":"Cell Wall","url":"https://www.academia.edu/Documents/in/Cell_Wall?f_ri=409525"},{"id":1010854,"name":"Complex Structure","url":"https://www.academia.edu/Documents/in/Complex_Structure?f_ri=409525"},{"id":1134595,"name":"Glycomics","url":"https://www.academia.edu/Documents/in/Glycomics?f_ri=409525"},{"id":1228946,"name":"Physical Properties","url":"https://www.academia.edu/Documents/in/Physical_Properties?f_ri=409525"},{"id":1974871,"name":"Oligosaccharides","url":"https://www.academia.edu/Documents/in/Oligosaccharides?f_ri=409525"},{"id":2369223,"name":"Nutritive Value","url":"https://www.academia.edu/Documents/in/Nutritive_Value?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_48534684" data-work_id="48534684" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48534684/Sequence_analyses_of_fimbriae_subunit_FimA_proteins_on_Actinomyces_naeslundii_genospecies_1_and_2_and_Actinomyces_odontolyticus_with_variant_carbohydrate_binding_specificities">Sequence analyses of fimbriae subunit FimA proteins on Actinomyces naeslundii genospecies 1 and 2 and Actinomyces odontolyticus with variant carbohydrate binding specificities</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Actinomyces naeslundii genospecies 1 and 2 express type-2 fimbriae (FimA subunit polymers) with variant Galbeta binding specificities and Actinomyces odontolyticus a sialic acid specificity to colonize different oral surfaces. However,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_48534684" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Actinomyces naeslundii genospecies 1 and 2 express type-2 fimbriae (FimA subunit polymers) with variant Galbeta binding specificities and Actinomyces odontolyticus a sialic acid specificity to colonize different oral surfaces. However, the fimbrial nature of the sialic acid binding property and sequence information about FimA proteins from multiple strains are lacking. Here we have sequenced fimA genes from strains of A.naeslundii genospecies 1 (n = 4) and genospecies 2 (n = 4), both of which harboured variant Galbeta-dependent hemagglutination (HA) types, and from A.odontolyticus PK984 with a sialic acid-dependent HA pattern. Three unique subtypes of FimA proteins with 63.8-66.4% sequence identity were present in strains of A. naeslundii genospecies 1 and 2 and A. odontolyticus. The generally high FimA sequence identity (&gt; 97.2%) within a genospecies revealed species specific sequences or segments that coincided with binding specificity. All three FimA protein variants contained...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48534684" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c68fa550adee8d5893358a42dd422f55" rel="nofollow" data-download="{"attachment_id":67096213,"asset_id":48534684,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/67096213/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="97818811" href="https://umu.academia.edu/AnnaBirve">Anna Birve</a><script data-card-contents-for-user="97818811" type="text/json">{"id":97818811,"first_name":"Anna","last_name":"Birve","domain_name":"umu","page_name":"AnnaBirve","display_name":"Anna Birve","profile_url":"https://umu.academia.edu/AnnaBirve?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_48534684 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="48534684"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 48534684, container: ".js-paper-rank-work_48534684", }); 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$(".js-view-count[data-work-id=48534684]").text(description); $(".js-view-count-work_48534684").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_48534684").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="48534684"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="596" href="https://www.academia.edu/Documents/in/Dentistry">Dentistry</a>, <script data-card-contents-for-ri="596" type="text/json">{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="37834" href="https://www.academia.edu/Documents/in/Western_blotting">Western blotting</a>, <script data-card-contents-for-ri="37834" type="text/json">{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="54433" href="https://www.academia.edu/Documents/in/Phylogeny">Phylogeny</a><script data-card-contents-for-ri="54433" type="text/json">{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48534684]'), work: {"id":48534684,"title":"Sequence analyses of fimbriae subunit FimA proteins on Actinomyces naeslundii genospecies 1 and 2 and Actinomyces odontolyticus with variant carbohydrate binding specificities","created_at":"2021-05-04T22:31:46.265-07:00","url":"https://www.academia.edu/48534684/Sequence_analyses_of_fimbriae_subunit_FimA_proteins_on_Actinomyces_naeslundii_genospecies_1_and_2_and_Actinomyces_odontolyticus_with_variant_carbohydrate_binding_specificities?f_ri=409525","dom_id":"work_48534684","summary":"Actinomyces naeslundii genospecies 1 and 2 express type-2 fimbriae (FimA subunit polymers) with variant Galbeta binding specificities and Actinomyces odontolyticus a sialic acid specificity to colonize different oral surfaces. However, the fimbrial nature of the sialic acid binding property and sequence information about FimA proteins from multiple strains are lacking. Here we have sequenced fimA genes from strains of A.naeslundii genospecies 1 (n = 4) and genospecies 2 (n = 4), both of which harboured variant Galbeta-dependent hemagglutination (HA) types, and from A.odontolyticus PK984 with a sialic acid-dependent HA pattern. Three unique subtypes of FimA proteins with 63.8-66.4% sequence identity were present in strains of A. naeslundii genospecies 1 and 2 and A. odontolyticus. The generally high FimA sequence identity (\u0026gt; 97.2%) within a genospecies revealed species specific sequences or segments that coincided with binding specificity. All three FimA protein variants contained...","downloadable_attachments":[{"id":67096213,"asset_id":48534684,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":97818811,"first_name":"Anna","last_name":"Birve","domain_name":"umu","page_name":"AnnaBirve","display_name":"Anna Birve","profile_url":"https://umu.academia.edu/AnnaBirve?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":596,"name":"Dentistry","url":"https://www.academia.edu/Documents/in/Dentistry?f_ri=409525","nofollow":false},{"id":37834,"name":"Western blotting","url":"https://www.academia.edu/Documents/in/Western_blotting?f_ri=409525","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525","nofollow":false},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny?f_ri=409525","nofollow":false},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment?f_ri=409525"},{"id":129391,"name":"Gene Order","url":"https://www.academia.edu/Documents/in/Gene_Order?f_ri=409525"},{"id":237568,"name":"Gram Positive Bacteria","url":"https://www.academia.edu/Documents/in/Gram_Positive_Bacteria?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":444298,"name":"Actinomyces","url":"https://www.academia.edu/Documents/in/Actinomyces?f_ri=409525"},{"id":462111,"name":"Western blot","url":"https://www.academia.edu/Documents/in/Western_blot?f_ri=409525"},{"id":687738,"name":"SIGNAL PEPTIDE","url":"https://www.academia.edu/Documents/in/SIGNAL_PEPTIDE?f_ri=409525"},{"id":759489,"name":"Sialic Acid","url":"https://www.academia.edu/Documents/in/Sialic_Acid?f_ri=409525"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity?f_ri=409525"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=409525"},{"id":990417,"name":"Recombinant Proteins","url":"https://www.academia.edu/Documents/in/Recombinant_Proteins?f_ri=409525"},{"id":1035619,"name":"Galactose Binding","url":"https://www.academia.edu/Documents/in/Galactose_Binding?f_ri=409525"},{"id":1188429,"name":"Hemagglutination","url":"https://www.academia.edu/Documents/in/Hemagglutination?f_ri=409525"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=409525"},{"id":2859152,"name":"BMC","url":"https://www.academia.edu/Documents/in/BMC?f_ri=409525"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_4285176" data-work_id="4285176" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4285176/Unprecedented_sugar_dependent_in_vivo_antitumor_activity_of_carbohydrate_pendant_cis_diamminedichloroplatinum_II_complexes">Unprecedented sugar-dependent in vivo antitumor activity of carbohydrate-pendant cis-diamminedichloroplatinum(II) complexes</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4285176" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span 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class="u-tcGrayDark u-fw700" data-has-card-for-user="5205472" href="https://independent.academia.edu/YoshieShinohara">Yoshie Shinohara</a><script data-card-contents-for-user="5205472" type="text/json">{"id":5205472,"first_name":"Yoshie","last_name":"Shinohara","domain_name":"independent","page_name":"YoshieShinohara","display_name":"Yoshie Shinohara","profile_url":"https://independent.academia.edu/YoshieShinohara?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_4285176 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4285176"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4285176, container: ".js-paper-rank-work_4285176", }); });</script></li><li class="js-percentile-work_4285176 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span 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$(".js-view-count[data-work-id=4285176]").text(description); $(".js-view-count-work_4285176").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_4285176").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="4285176"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">10</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="531" href="https://www.academia.edu/Documents/in/Organic_Chemistry">Organic Chemistry</a>, <script data-card-contents-for-ri="531" type="text/json">{"id":531,"name":"Organic Chemistry","url":"https://www.academia.edu/Documents/in/Organic_Chemistry?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="21732" href="https://www.academia.edu/Documents/in/Magnetic_Resonance_Spectroscopy">Magnetic Resonance Spectroscopy</a>, <script data-card-contents-for-ri="21732" type="text/json">{"id":21732,"name":"Magnetic Resonance Spectroscopy","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Spectroscopy?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="84760" href="https://www.academia.edu/Documents/in/Mice">Mice</a>, <script data-card-contents-for-ri="84760" type="text/json">{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="276781" href="https://www.academia.edu/Documents/in/Cisplatin">Cisplatin</a><script data-card-contents-for-ri="276781" type="text/json">{"id":276781,"name":"Cisplatin","url":"https://www.academia.edu/Documents/in/Cisplatin?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=4285176]'), work: {"id":4285176,"title":"Unprecedented sugar-dependent in vivo antitumor activity of carbohydrate-pendant cis-diamminedichloroplatinum(II) complexes","created_at":"2013-08-20T18:54:17.720-07:00","url":"https://www.academia.edu/4285176/Unprecedented_sugar_dependent_in_vivo_antitumor_activity_of_carbohydrate_pendant_cis_diamminedichloroplatinum_II_complexes?f_ri=409525","dom_id":"work_4285176","summary":null,"downloadable_attachments":[{"id":49959546,"asset_id":4285176,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5205472,"first_name":"Yoshie","last_name":"Shinohara","domain_name":"independent","page_name":"YoshieShinohara","display_name":"Yoshie Shinohara","profile_url":"https://independent.academia.edu/YoshieShinohara?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":531,"name":"Organic Chemistry","url":"https://www.academia.edu/Documents/in/Organic_Chemistry?f_ri=409525","nofollow":false},{"id":21732,"name":"Magnetic Resonance Spectroscopy","url":"https://www.academia.edu/Documents/in/Magnetic_Resonance_Spectroscopy?f_ri=409525","nofollow":false},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=409525","nofollow":false},{"id":276781,"name":"Cisplatin","url":"https://www.academia.edu/Documents/in/Cisplatin?f_ri=409525","nofollow":false},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":609249,"name":"CARBOHYDRATES","url":"https://www.academia.edu/Documents/in/CARBOHYDRATES?f_ri=409525"},{"id":802213,"name":"Lethal Dose","url":"https://www.academia.edu/Documents/in/Lethal_Dose?f_ri=409525"},{"id":920744,"name":"Antitumor Activity","url":"https://www.academia.edu/Documents/in/Antitumor_Activity?f_ri=409525"},{"id":1212103,"name":"Antineoplastic Agents","url":"https://www.academia.edu/Documents/in/Antineoplastic_Agents?f_ri=409525"},{"id":1724844,"name":"Molecular Structure","url":"https://www.academia.edu/Documents/in/Molecular_Structure?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_29201881" data-work_id="29201881" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/29201881/Energy_expenditure_and_substrate_oxidation_during_and_after_eccentric_cycling">Energy expenditure and substrate oxidation during and after eccentric cycling</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button 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Metabolism</a>, <script data-card-contents-for-ri="36213" type="text/json">{"id":36213,"name":"Energy Metabolism","url":"https://www.academia.edu/Documents/in/Energy_Metabolism?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="135185" href="https://www.academia.edu/Documents/in/Exercise">Exercise</a>, <script data-card-contents-for-ri="135185" type="text/json">{"id":135185,"name":"Exercise","url":"https://www.academia.edu/Documents/in/Exercise?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="184609" href="https://www.academia.edu/Documents/in/Lipid_metabolism">Lipid metabolism</a>, <script data-card-contents-for-ri="184609" type="text/json">{"id":184609,"name":"Lipid metabolism","url":"https://www.academia.edu/Documents/in/Lipid_metabolism?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="409525" href="https://www.academia.edu/Documents/in/Carbohydrate_metabolism">Carbohydrate metabolism</a><script data-card-contents-for-ri="409525" type="text/json">{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=29201881]'), work: {"id":29201881,"title":"Energy expenditure and substrate oxidation during and after eccentric cycling","created_at":"2016-10-16T19:41:06.377-07:00","url":"https://www.academia.edu/29201881/Energy_expenditure_and_substrate_oxidation_during_and_after_eccentric_cycling?f_ri=409525","dom_id":"work_29201881","summary":null,"downloadable_attachments":[{"id":49651146,"asset_id":29201881,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":33994756,"first_name":"Anthony","last_name":"Blazevich","domain_name":"edithcowan","page_name":"AnthonyBlazevich","display_name":"Anthony J Blazevich","profile_url":"https://edithcowan.academia.edu/AnthonyBlazevich?f_ri=409525","photo":"https://0.academia-photos.com/33994756/14499607/15384234/s65_anthony.blazevich.jpg"}],"research_interests":[{"id":36213,"name":"Energy Metabolism","url":"https://www.academia.edu/Documents/in/Energy_Metabolism?f_ri=409525","nofollow":false},{"id":135185,"name":"Exercise","url":"https://www.academia.edu/Documents/in/Exercise?f_ri=409525","nofollow":false},{"id":184609,"name":"Lipid metabolism","url":"https://www.academia.edu/Documents/in/Lipid_metabolism?f_ri=409525","nofollow":false},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_5462789" data-work_id="5462789" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/5462789/Arabinoxylan_oligosaccharides_AXOS_affect_the_protein_carbohydrate_fermentation_balance_and_microbial_population_dynamics_of_the_Simulator_of_Human_Intestinal_Microbial_Ecosystem">Arabinoxylan-oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the Simulator of Human Intestinal Microbial Ecosystem</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Arabinoxylan-oligosaccharides (AXOS) are a recently newly discovered class of candidate prebiotics as – depending on their structure – they are fermented in different regions of gastrointestinal tract. This can have an impact on the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5462789" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Arabinoxylan-oligosaccharides (AXOS) are a recently newly discovered class of candidate prebiotics as – depending on their structure – they are fermented in different regions of gastrointestinal tract. This can have an impact on the protein/carbohydrate fermentation balance in the large intestine and, thus, affect the generation of potentially toxic metabolites in the colon originating from proteolytic activity. In this study, we screened different AXOS preparations for their impact on the in vitro intestinal fermentation activity and microbial community structure. Short-term fermentation experiments with AXOS with an average degree of polymerization (avDP) of 29 allowed part of the oligosaccharides to reach the distal colon, and decreased the concentration of proteolytic markers, whereas AXOS with lower avDP were primarily fermented in the proximal colon. Additionally, prolonged supplementation of AXOS with avDP 29 to the Simulator of Human Intestinal Microbial Ecosystem (SHIME) reactor decreased levels of the toxic proteolytic markers phenol and p-cresol in the two distal colon compartments and increased concentrations of beneficial short-chain fatty acids (SCFA) in all colon vessels (25–48%). Denaturant gradient gel electrophoresis (DGGE) analysis indicated that AXOS supplementation only slightly modified the total microbial community, implying that the observed effects on fermentation markers are mainly caused by changes in fermentation activity. Finally, specific quantitative PCR (qPCR) analysis showed that AXOS supplementation significantly increased the amount of health-promoting lactobacilli as well as of Bacteroides–Prevotella and Clostridium coccoides–Eubacterium rectale groups. These data allow concluding that AXOS are promising candidates to modulate the microbial metabolism in the distal colon.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5462789" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="47d6abf373aa478d56b37703a3473842" rel="nofollow" data-download="{"attachment_id":49283430,"asset_id":5462789,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49283430/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="7668021" href="https://independent.academia.edu/MassimoMarzorati">Massimo Marzorati</a><script data-card-contents-for-user="7668021" type="text/json">{"id":7668021,"first_name":"Massimo","last_name":"Marzorati","domain_name":"independent","page_name":"MassimoMarzorati","display_name":"Massimo Marzorati","profile_url":"https://independent.academia.edu/MassimoMarzorati?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_5462789 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5462789"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5462789, container: ".js-paper-rank-work_5462789", }); 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This can have an impact on the protein/carbohydrate fermentation balance in the large intestine and, thus, affect the generation of potentially toxic metabolites in the colon originating from proteolytic activity. In this study, we screened different AXOS preparations for their impact on the in vitro intestinal fermentation activity and microbial community structure. Short-term fermentation experiments with AXOS with an average degree of polymerization (avDP) of 29 allowed part of the oligosaccharides to reach the distal colon, and decreased the concentration of proteolytic markers, whereas AXOS with lower avDP were primarily fermented in the proximal colon. Additionally, prolonged supplementation of AXOS with avDP 29 to the Simulator of Human Intestinal Microbial Ecosystem (SHIME) reactor decreased levels of the toxic proteolytic markers phenol and p-cresol in the two distal colon compartments and increased concentrations of beneficial short-chain fatty acids (SCFA) in all colon vessels (25–48%). Denaturant gradient gel electrophoresis (DGGE) analysis indicated that AXOS supplementation only slightly modified the total microbial community, implying that the observed effects on fermentation markers are mainly caused by changes in fermentation activity. Finally, specific quantitative PCR (qPCR) analysis showed that AXOS supplementation significantly increased the amount of health-promoting lactobacilli as well as of Bacteroides–Prevotella and Clostridium coccoides–Eubacterium rectale groups. These data allow concluding that AXOS are promising candidates to modulate the microbial metabolism in the distal colon.","downloadable_attachments":[{"id":49283430,"asset_id":5462789,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":7668021,"first_name":"Massimo","last_name":"Marzorati","domain_name":"independent","page_name":"MassimoMarzorati","display_name":"Massimo Marzorati","profile_url":"https://independent.academia.edu/MassimoMarzorati?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false},{"id":16645,"name":"Microbial biotechnology","url":"https://www.academia.edu/Documents/in/Microbial_biotechnology?f_ri=409525","nofollow":false},{"id":18329,"name":"Prebiotics","url":"https://www.academia.edu/Documents/in/Prebiotics?f_ri=409525","nofollow":false},{"id":32909,"name":"Polymerization","url":"https://www.academia.edu/Documents/in/Polymerization?f_ri=409525","nofollow":false},{"id":48579,"name":"Microbial Biotechnology","url":"https://www.academia.edu/Documents/in/Microbial_Biotechnology-1?f_ri=409525"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=409525"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=409525"},{"id":269129,"name":"Fermentation","url":"https://www.academia.edu/Documents/in/Fermentation?f_ri=409525"},{"id":291136,"name":"Intestines","url":"https://www.academia.edu/Documents/in/Intestines?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":592052,"name":"Xylans","url":"https://www.academia.edu/Documents/in/Xylans?f_ri=409525"},{"id":1237825,"name":"Population dynamic","url":"https://www.academia.edu/Documents/in/Population_dynamic?f_ri=409525"},{"id":1974871,"name":"Oligosaccharides","url":"https://www.academia.edu/Documents/in/Oligosaccharides?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_8711463" data-work_id="8711463" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/8711463/Preparation_and_Analysis_of_an_Expressed_Sequence_Tag_Library_from_the_Toxic_Dinoflagellate_Alexandrium_catenella">Preparation and Analysis of an Expressed Sequence Tag Library from the Toxic Dinoflagellate Alexandrium catenella</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_8711463" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Dinoflagellates of the genus Alexandrium are photosynthetic microalgae that have an extreme importance due to the impact of some toxic species on shellfish aquaculture industry. Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357–464203).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/8711463" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5c6b3fd49d8d665c4967a483b843f768" rel="nofollow" data-download="{"attachment_id":48014504,"asset_id":8711463,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48014504/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="18291684" href="https://independent.academia.edu/DanielaFuentes6">Daniela Fuentes</a><script data-card-contents-for-user="18291684" type="text/json">{"id":18291684,"first_name":"Daniela","last_name":"Fuentes","domain_name":"independent","page_name":"DanielaFuentes6","display_name":"Daniela Fuentes","profile_url":"https://independent.academia.edu/DanielaFuentes6?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_8711463 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="8711463"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 8711463, container: ".js-paper-rank-work_8711463", }); 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Alexandrium catenella is the species responsible for the production of paralytic shellfish poisoning in Chile and other geographical areas. We have constructed a cDNA library from midexponential cells of A. catenella grown in culture free of associated bacteria and sequenced 10,850 expressed sequence tags (ESTs) that were assembled into 1,021 contigs and 5,475 singletons for a total of 6,496 unigenes. Approximately 41.6% of the unigenes showed similarity to genes with predicted function. A significant number of unigenes showed similarity with genes from other dinoflagellates, plants, and other protists. Among the identified genes, the most expressed correspond to those coding for proteins of luminescence, carbohydrate metabolism, and photosynthesis. The sequences of 9,847 ESTs have been deposited in Gene Bank (accession numbers EX 454357–464203).","downloadable_attachments":[{"id":48014504,"asset_id":8711463,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":18291684,"first_name":"Daniela","last_name":"Fuentes","domain_name":"independent","page_name":"DanielaFuentes6","display_name":"Daniela Fuentes","profile_url":"https://independent.academia.edu/DanielaFuentes6?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=409525","nofollow":false},{"id":3216,"name":"Genomics","url":"https://www.academia.edu/Documents/in/Genomics?f_ri=409525","nofollow":false},{"id":23836,"name":"Harmful algal blooms","url":"https://www.academia.edu/Documents/in/Harmful_algal_blooms?f_ri=409525","nofollow":false},{"id":58054,"name":"Environmental 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catenella","url":"https://www.academia.edu/Documents/in/Alexandrium_catenella?f_ri=409525"},{"id":505334,"name":"Red tides","url":"https://www.academia.edu/Documents/in/Red_tides?f_ri=409525"},{"id":1578793,"name":"cDNA library","url":"https://www.academia.edu/Documents/in/cDNA_library?f_ri=409525"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14572582 coauthored" data-work_id="14572582" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" 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type="text/json">{"id":33532106,"first_name":"Bogdan","last_name":"Dolezych","domain_name":"silesian","page_name":"BogdanDolezych","display_name":"Bogdan Dolezych","profile_url":"https://silesian.academia.edu/BogdanDolezych?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-14572582">+2</span><div class="hidden js-additional-users-14572582"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://sum.academia.edu/Rafa%C5%82Bu%C5%82dak">Rafał Bułdak</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/TomekSawczyn">Tomek Sawczyn</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-14572582'), placement: 'bottom', hide_delay: 200, 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type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="112324" href="https://www.academia.edu/Documents/in/Pseudomonas">Pseudomonas</a>, <script data-card-contents-for-ri="112324" type="text/json">{"id":112324,"name":"Pseudomonas","url":"https://www.academia.edu/Documents/in/Pseudomonas?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="188240" href="https://www.academia.edu/Documents/in/Tomato">Tomato</a><script 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The metabolic consequences of this signalling pathway are not known. We reduced the basal level of InsP3 in tomato (Solanum... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_4022000" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Inositol-(1,4,5)-trisphosphate (InsP3) is a second messenger in plants that increases in response to many stimuli. The metabolic consequences of this signalling pathway are not known. We reduced the basal level of InsP3 in tomato (Solanum lycopersicum cv. Micro-Tom) by expressing the human type I inositol polyphosphate 5-phosphatase (InsP 5-ptase) gene. Transgenic lines producing InsP 5-ptase protein had between 15% and 30% of the basal InsP3 level of control plants. This increased hydrolysis of InsP3 caused dramatic increases in drought tolerance, vegetative biomass and lycopene and hexose concentrations in the fruits. Transcript profiling of root, leaf and fruit tissues identified a small group of genes, including a cell-wall invertase inhibitor gene, that were differentially regulated in all tissues of the InsP 5-ptase expressing plants. Significant differences were found in the amounts of carbohydrates and organic phosphate in these plants. Plants with increased hydrolysis of InsP3 in the cytosol also showed increased net CO2-fixation and sucrose export into sink tissue and storage of hexoses in the source leaves. The increase in biomass was dependent on the supply of inorganic phosphate in the nutrient medium. Uptake and storage of phosphate was increased in the transgene expressing lines. This suggests that in tomato, increased flux through the inositol phosphate pathway uncoupled phosphate sensing from phosphate metabolism. Altering the second messenger, InsP3, revealed multiple coordinated changes in development and metabolism in tomato that have potential for crop improvement.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4022000" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="10c758c6c50e17bddc3daaaf9b471096" rel="nofollow" data-download="{"attachment_id":50078039,"asset_id":4022000,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50078039/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4831800" href="https://ncsu.academia.edu/HeikeSederoff">Heike Sederoff</a><script data-card-contents-for-user="4831800" type="text/json">{"id":4831800,"first_name":"Heike","last_name":"Sederoff","domain_name":"ncsu","page_name":"HeikeSederoff","display_name":"Heike Sederoff","profile_url":"https://ncsu.academia.edu/HeikeSederoff?f_ri=409525","photo":"https://0.academia-photos.com/4831800/2065415/2431352/s65_heike.sederoff.jpg"}</script></span></span></li><li class="js-paper-rank-work_4022000 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4022000"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4022000, container: ".js-paper-rank-work_4022000", }); 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The metabolic consequences of this signalling pathway are not known. We reduced the basal level of InsP3 in tomato (Solanum lycopersicum cv. Micro-Tom) by expressing the human type I inositol polyphosphate 5-phosphatase (InsP 5-ptase) gene. Transgenic lines producing InsP 5-ptase protein had between 15% and 30% of the basal InsP3 level of control plants. This increased hydrolysis of InsP3 caused dramatic increases in drought tolerance, vegetative biomass and lycopene and hexose concentrations in the fruits. Transcript profiling of root, leaf and fruit tissues identified a small group of genes, including a cell-wall invertase inhibitor gene, that were differentially regulated in all tissues of the InsP 5-ptase expressing plants. Significant differences were found in the amounts of carbohydrates and organic phosphate in these plants. Plants with increased hydrolysis of InsP3 in the cytosol also showed increased net CO2-fixation and sucrose export into sink tissue and storage of hexoses in the source leaves. The increase in biomass was dependent on the supply of inorganic phosphate in the nutrient medium. Uptake and storage of phosphate was increased in the transgene expressing lines. This suggests that in tomato, increased flux through the inositol phosphate pathway uncoupled phosphate sensing from phosphate metabolism. Altering the second messenger, InsP3, revealed multiple coordinated changes in development and metabolism in tomato that have potential for crop improvement.","downloadable_attachments":[{"id":50078039,"asset_id":4022000,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4831800,"first_name":"Heike","last_name":"Sederoff","domain_name":"ncsu","page_name":"HeikeSederoff","display_name":"Heike Sederoff","profile_url":"https://ncsu.academia.edu/HeikeSederoff?f_ri=409525","photo":"https://0.academia-photos.com/4831800/2065415/2431352/s65_heike.sederoff.jpg"}],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=409525","nofollow":false},{"id":5411,"name":"Biomass","url":"https://www.academia.edu/Documents/in/Biomass?f_ri=409525","nofollow":false},{"id":18225,"name":"Plant biotechnology","url":"https://www.academia.edu/Documents/in/Plant_biotechnology?f_ri=409525","nofollow":false},{"id":22053,"name":"Carotenoids","url":"https://www.academia.edu/Documents/in/Carotenoids?f_ri=409525","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525"},{"id":160814,"name":"Fruit","url":"https://www.academia.edu/Documents/in/Fruit?f_ri=409525"},{"id":186792,"name":"Droughts","url":"https://www.academia.edu/Documents/in/Droughts?f_ri=409525"},{"id":188240,"name":"Tomato","url":"https://www.academia.edu/Documents/in/Tomato?f_ri=409525"},{"id":283029,"name":"Drought tolerance","url":"https://www.academia.edu/Documents/in/Drought_tolerance?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":1030794,"name":"Hydrolysis","url":"https://www.academia.edu/Documents/in/Hydrolysis?f_ri=409525"},{"id":1640340,"name":"Lycopersicon esculentum","url":"https://www.academia.edu/Documents/in/Lycopersicon_esculentum?f_ri=409525"},{"id":1810445,"name":"Gene expression profiling","url":"https://www.academia.edu/Documents/in/Gene_expression_profiling?f_ri=409525"},{"id":1905343,"name":"Plant Leaves","url":"https://www.academia.edu/Documents/in/Plant_Leaves?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_76055219" data-work_id="76055219" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/76055219/Sucrose_utilization_and_mineral_nutrient_uptake_during_hairy_root_growth_of_red_beet_Beta_vulgaris_L_in_liquid_culture">Sucrose utilization and mineral nutrient uptake during hairy root growth of red beet ( Beta vulgaris L.) in liquid culture</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Information concerning the sugar status of plant cells is of greatimportance during all stages of the plant life cycle. The aim of this work wasto study primary carbohydrate metabolism in hairy roots of red beet. Growth ofhairy roots of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_76055219" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Information concerning the sugar status of plant cells is of greatimportance during all stages of the plant life cycle. The aim of this work wasto study primary carbohydrate metabolism in hairy roots of red beet. Growth ofhairy roots of red beet in vitro and changes in concentration of major nutrientsand sugar in the media were measured over a growth cycle</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/76055219" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3f2248286111931002fe634c790438aa" rel="nofollow" data-download="{"attachment_id":83740335,"asset_id":76055219,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/83740335/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="65785474" href="https://independent.academia.edu/kpaek">kee yoeup paek</a><script data-card-contents-for-user="65785474" type="text/json">{"id":65785474,"first_name":"kee yoeup","last_name":"paek","domain_name":"independent","page_name":"kpaek","display_name":"kee yoeup paek","profile_url":"https://independent.academia.edu/kpaek?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_76055219 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="76055219"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 76055219, container: ".js-paper-rank-work_76055219", }); 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The aim of this work wasto study primary carbohydrate metabolism in hairy roots of red beet. 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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/48601985/Limitations_of_thermophilic_anaerobic_wastewater_treatment_and_the_consequences_for_process_design">Limitations of thermophilic anaerobic wastewater treatment and the consequences for process design</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/48601985" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1c1c07fc4b332b9c6a2496e799ff1d18" rel="nofollow" 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window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_48601985"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_48601985 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="48601985"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 48601985; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=48601985]").text(description); $(".js-view-count-work_48601985").attr('title', description).tooltip(); }); });</script></span><script>$(function() { 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type="text/json">{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5411" href="https://www.academia.edu/Documents/in/Biomass">Biomass</a>, <script data-card-contents-for-ri="5411" type="text/json">{"id":5411,"name":"Biomass","url":"https://www.academia.edu/Documents/in/Biomass?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a><script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=48601985]'), work: {"id":48601985,"title":"Limitations of thermophilic anaerobic wastewater treatment and the consequences for process design","created_at":"2021-05-05T00:18:21.537-07:00","url":"https://www.academia.edu/48601985/Limitations_of_thermophilic_anaerobic_wastewater_treatment_and_the_consequences_for_process_design?f_ri=409525","dom_id":"work_48601985","summary":null,"downloadable_attachments":[{"id":67128930,"asset_id":48601985,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":27587500,"first_name":"jules","last_name":"van Lier","domain_name":"independent","page_name":"julesvanLier","display_name":"jules van Lier","profile_url":"https://independent.academia.edu/julesvanLier?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false},{"id":5398,"name":"Biotechnology","url":"https://www.academia.edu/Documents/in/Biotechnology?f_ri=409525","nofollow":false},{"id":5411,"name":"Biomass","url":"https://www.academia.edu/Documents/in/Biomass?f_ri=409525","nofollow":false},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=409525","nofollow":false},{"id":9991,"name":"Wastewater Treatment","url":"https://www.academia.edu/Documents/in/Wastewater_Treatment?f_ri=409525"},{"id":28157,"name":"Environmental Change","url":"https://www.academia.edu/Documents/in/Environmental_Change?f_ri=409525"},{"id":59113,"name":"Anaerobic Digestion","url":"https://www.academia.edu/Documents/in/Anaerobic_Digestion?f_ri=409525"},{"id":74043,"name":"Waste water treatment","url":"https://www.academia.edu/Documents/in/Waste_water_treatment?f_ri=409525"},{"id":99040,"name":"Process Design","url":"https://www.academia.edu/Documents/in/Process_Design?f_ri=409525"},{"id":104501,"name":"Anaerobic Bacteria","url":"https://www.academia.edu/Documents/in/Anaerobic_Bacteria?f_ri=409525"},{"id":131994,"name":"Laboratory experiment","url":"https://www.academia.edu/Documents/in/Laboratory_experiment?f_ri=409525"},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":661887,"name":"Chemical Oxygen Demand","url":"https://www.academia.edu/Documents/in/Chemical_Oxygen_Demand?f_ri=409525"},{"id":1912509,"name":"Production Rate","url":"https://www.academia.edu/Documents/in/Production_Rate?f_ri=409525"},{"id":3207127,"name":"Antonie van Leeuwenhoek","url":"https://www.academia.edu/Documents/in/Antonie_van_Leeuwenhoek?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_47660560" data-work_id="47660560" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/47660560/How_does_the_body_deal_with_energy_from_alcohol">How does the body deal with energy from alcohol?</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" 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InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="124492046" href="https://independent.academia.edu/BenjaminBuemann">Benjamin Buemann</a><script data-card-contents-for-user="124492046" type="text/json">{"id":124492046,"first_name":"Benjamin","last_name":"Buemann","domain_name":"independent","page_name":"BenjaminBuemann","display_name":"Benjamin Buemann","profile_url":"https://independent.academia.edu/BenjaminBuemann?f_ri=409525","photo":"https://0.academia-photos.com/124492046/34561136/35684555/s65_benjamin.buemann.jpg"}</script></span></span></li><li class="js-paper-rank-work_47660560 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="47660560"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new 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type="text/json">{"id":1907,"name":"Nutrition","url":"https://www.academia.edu/Documents/in/Nutrition?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="36213" href="https://www.academia.edu/Documents/in/Energy_Metabolism">Energy Metabolism</a>, <script data-card-contents-for-ri="36213" type="text/json">{"id":36213,"name":"Energy Metabolism","url":"https://www.academia.edu/Documents/in/Energy_Metabolism?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="91863" href="https://www.academia.edu/Documents/in/Alcoholism">Alcoholism</a>, <script data-card-contents-for-ri="91863" type="text/json">{"id":91863,"name":"Alcoholism","url":"https://www.academia.edu/Documents/in/Alcoholism?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="110254" href="https://www.academia.edu/Documents/in/Alcohol_Drinking">Alcohol Drinking</a><script data-card-contents-for-ri="110254" type="text/json">{"id":110254,"name":"Alcohol Drinking","url":"https://www.academia.edu/Documents/in/Alcohol_Drinking?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=47660560]'), work: {"id":47660560,"title":"How does the body deal with energy from alcohol?","created_at":"2021-04-23T12:26:57.529-07:00","url":"https://www.academia.edu/47660560/How_does_the_body_deal_with_energy_from_alcohol?f_ri=409525","dom_id":"work_47660560","summary":null,"downloadable_attachments":[{"id":66638456,"asset_id":47660560,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":124492046,"first_name":"Benjamin","last_name":"Buemann","domain_name":"independent","page_name":"BenjaminBuemann","display_name":"Benjamin Buemann","profile_url":"https://independent.academia.edu/BenjaminBuemann?f_ri=409525","photo":"https://0.academia-photos.com/124492046/34561136/35684555/s65_benjamin.buemann.jpg"}],"research_interests":[{"id":1907,"name":"Nutrition","url":"https://www.academia.edu/Documents/in/Nutrition?f_ri=409525","nofollow":false},{"id":36213,"name":"Energy Metabolism","url":"https://www.academia.edu/Documents/in/Energy_Metabolism?f_ri=409525","nofollow":false},{"id":91863,"name":"Alcoholism","url":"https://www.academia.edu/Documents/in/Alcoholism?f_ri=409525","nofollow":false},{"id":110254,"name":"Alcohol Drinking","url":"https://www.academia.edu/Documents/in/Alcohol_Drinking?f_ri=409525","nofollow":false},{"id":121705,"name":"Ethanol","url":"https://www.academia.edu/Documents/in/Ethanol?f_ri=409525"},{"id":213897,"name":"Phenotype","url":"https://www.academia.edu/Documents/in/Phenotype?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":450153,"name":"Fats","url":"https://www.academia.edu/Documents/in/Fats?f_ri=409525"},{"id":564878,"name":"Body Weight","url":"https://www.academia.edu/Documents/in/Body_Weight?f_ri=409525"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=409525"},{"id":1567539,"name":"Energy Intake","url":"https://www.academia.edu/Documents/in/Energy_Intake?f_ri=409525"},{"id":2369223,"name":"Nutritive Value","url":"https://www.academia.edu/Documents/in/Nutritive_Value?f_ri=409525"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_2161565" data-work_id="2161565" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/2161565/Metabolic_pathways_in_tropical_dicotyledonous_albuminous_seeds_Coffea_arabica_as_a_case_study">Metabolic pathways in tropical dicotyledonous albuminous seeds: Coffea arabica as a case study</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The genomic era facilitates the understanding of how transcriptional networks are interconnected to program seed development and filling. However, to date, little information is available regarding dicot seeds with a transient perisperm... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_2161565" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The genomic era facilitates the understanding of how transcriptional networks are interconnected to program seed development and filling. However, to date, little information is available regarding dicot seeds with a transient perisperm and a persistent, copious endosperm. Coffea arabica is the subject of increasing genomic research and is a model for nonorthodox albuminous dicot seeds of tropical origin.The aim of this study was to reconstruct the metabolic pathways involved in the biosynthesis of the main coffee seed storage compounds, namely cell wall polysaccharides, triacylglycerols, sucrose, and chlorogenic acids. For this purpose, we integrated transcriptomic and metabolite analyses, combining real-time RT-PCR performed on 137 selected genes (of which 79 were uncharacterized in Coffea) and metabolite profiling.Our map-drawing approach derived from model plants enabled us to propose a rationale for the peculiar traits of the coffee endosperm, such as its unusual fatty acid composition, remarkable accumulation of chlorogenic acid and cell wall polysaccharides.Comparison with the developmental features of exalbuminous seeds described in the literature revealed that the two seed types share important regulatory mechanisms for reserve biosynthesis, independent of the origin and ploidy level of the storage tissue.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/2161565" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="82138a8a5649e807f8ec108738aa5cc7" rel="nofollow" data-download="{"attachment_id":50729507,"asset_id":2161565,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50729507/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2787301" href="https://gulbenkian.academia.edu/JordiSalmona">Jordi Salmona</a><script data-card-contents-for-user="2787301" type="text/json">{"id":2787301,"first_name":"Jordi","last_name":"Salmona","domain_name":"gulbenkian","page_name":"JordiSalmona","display_name":"Jordi Salmona","profile_url":"https://gulbenkian.academia.edu/JordiSalmona?f_ri=409525","photo":"https://0.academia-photos.com/2787301/911271/1138902/s65_jordi.salmona.jpg"}</script></span></span></li><li class="js-paper-rank-work_2161565 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="2161565"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 2161565, container: ".js-paper-rank-work_2161565", }); 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However, to date, little information is available regarding dicot seeds with a transient perisperm and a persistent, copious endosperm. Coffea arabica is the subject of increasing genomic research and is a model for nonorthodox albuminous dicot seeds of tropical origin.The aim of this study was to reconstruct the metabolic pathways involved in the biosynthesis of the main coffee seed storage compounds, namely cell wall polysaccharides, triacylglycerols, sucrose, and chlorogenic acids. For this purpose, we integrated transcriptomic and metabolite analyses, combining real-time RT-PCR performed on 137 selected genes (of which 79 were uncharacterized in Coffea) and metabolite profiling.Our map-drawing approach derived from model plants enabled us to propose a rationale for the peculiar traits of the coffee endosperm, such as its unusual fatty acid composition, remarkable accumulation of chlorogenic acid and cell wall polysaccharides.Comparison with the developmental features of exalbuminous seeds described in the literature revealed that the two seed types share important regulatory mechanisms for reserve biosynthesis, independent of the origin and ploidy level of the storage tissue.","downloadable_attachments":[{"id":50729507,"asset_id":2161565,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2787301,"first_name":"Jordi","last_name":"Salmona","domain_name":"gulbenkian","page_name":"JordiSalmona","display_name":"Jordi Salmona","profile_url":"https://gulbenkian.academia.edu/JordiSalmona?f_ri=409525","photo":"https://0.academia-photos.com/2787301/911271/1138902/s65_jordi.salmona.jpg"}],"research_interests":[{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism?f_ri=409525","nofollow":false},{"id":16062,"name":"Starch","url":"https://www.academia.edu/Documents/in/Starch?f_ri=409525","nofollow":false},{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression?f_ri=409525","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525","nofollow":false},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids?f_ri=409525"},{"id":96047,"name":"Case 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metabolism","url":"https://www.academia.edu/Documents/in/Lipid_metabolism?f_ri=409525"},{"id":233229,"name":"Genes","url":"https://www.academia.edu/Documents/in/Genes?f_ri=409525"},{"id":386438,"name":"Seeds","url":"https://www.academia.edu/Documents/in/Seeds?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":713144,"name":"Biochemical Pathways","url":"https://www.academia.edu/Documents/in/Biochemical_Pathways?f_ri=409525"},{"id":756579,"name":"Coffea arabica","url":"https://www.academia.edu/Documents/in/Coffea_arabica?f_ri=409525"},{"id":764799,"name":"Metabolic pathway","url":"https://www.academia.edu/Documents/in/Metabolic_pathway?f_ri=409525"},{"id":906876,"name":"Tropical Climate","url":"https://www.academia.edu/Documents/in/Tropical_Climate?f_ri=409525"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=409525"},{"id":1356430,"name":"Chlorogenic Acid","url":"https://www.academia.edu/Documents/in/Chlorogenic_Acid?f_ri=409525"},{"id":1436039,"name":"Plant Oils","url":"https://www.academia.edu/Documents/in/Plant_Oils?f_ri=409525"},{"id":1447809,"name":"Coffea","url":"https://www.academia.edu/Documents/in/Coffea?f_ri=409525"},{"id":1775002,"name":"Metabolic Pathways","url":"https://www.academia.edu/Documents/in/Metabolic_Pathways?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_8018956" data-work_id="8018956" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest 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Bacteria","url":"https://www.academia.edu/Documents/in/Gram_Positive_Bacteria?f_ri=409525"},{"id":351776,"name":"Actinomycetales","url":"https://www.academia.edu/Documents/in/Actinomycetales?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":479537,"name":"Bacterial growth","url":"https://www.academia.edu/Documents/in/Bacterial_growth?f_ri=409525"},{"id":585595,"name":"Molecular Mass","url":"https://www.academia.edu/Documents/in/Molecular_Mass?f_ri=409525"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity?f_ri=409525"},{"id":1089035,"name":"Succinic Acid","url":"https://www.academia.edu/Documents/in/Succinic_Acid?f_ri=409525"},{"id":2153835,"name":"Enzyme Induction","url":"https://www.academia.edu/Documents/in/Enzyme_Induction?f_ri=409525"},{"id":2231954,"name":"Natural rubber 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href="https://www.academia.edu/Documents/in/Molecular_Dynamics_Simulation">Molecular Dynamics Simulation</a>, <script data-card-contents-for-ri="2736" type="text/json">{"id":2736,"name":"Molecular Dynamics Simulation","url":"https://www.academia.edu/Documents/in/Molecular_Dynamics_Simulation?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9032" href="https://www.academia.edu/Documents/in/Interaction">Interaction</a>, <script data-card-contents-for-ri="9032" type="text/json">{"id":9032,"name":"Interaction","url":"https://www.academia.edu/Documents/in/Interaction?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="10640" href="https://www.academia.edu/Documents/in/Drug_Discovery">Drug Discovery</a>, <script data-card-contents-for-ri="10640" type="text/json">{"id":10640,"name":"Drug Discovery","url":"https://www.academia.edu/Documents/in/Drug_Discovery?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="48636" href="https://www.academia.edu/Documents/in/Simulation">Simulation</a><script data-card-contents-for-ri="48636" type="text/json">{"id":48636,"name":"Simulation","url":"https://www.academia.edu/Documents/in/Simulation?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=4760308]'), work: {"id":4760308,"title":"Molecular simulations of carbohydrates and protein–carbohydrate interactions: motivation, issues and 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metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":609249,"name":"CARBOHYDRATES","url":"https://www.academia.edu/Documents/in/CARBOHYDRATES?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_25422450" data-work_id="25422450" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/25422450/Elevated_Atmospheric_CO2_Concentration_Enhances_Carbohydrate_Metabolism_in_Developing_Lycopersicon_esculentum_Mill_Cultivars">Elevated Atmospheric CO2 Concentration Enhances Carbohydrate Metabolism in Developing Lycopersicon esculentum Mill. Cultivars</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This study examined the influence of preharvest elevated atmospheric CO2 concentration (850 ± 50 ppm) on the fruit growth, total soluble solids (TSS), fructose, glucose, sucrose, soluble and cell wall-bound acid invertase (AI)... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_25422450" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This study examined the influence of preharvest elevated atmospheric CO2 concentration (850 ± 50 ppm) on the fruit growth, total soluble solids (TSS), fructose, glucose, sucrose, soluble and cell wall-bound acid invertase (AI) (ß-fructofuranoside fructohydrolase, EC 3. 2. 1. 26), sucrose synthase (SuSy) (UDP glucose: D-fructose 2 -glucosyltransferase, EC 2. 4. 1. 13) and sucrose phosphate synthase (SPS) (UDP glucose: D-fructose-6 -phosphate 2 -glucosyltransferase, EC 2. 4. 1. 14) activities of domesticated tomatoes (Lycopersicon esculentum Mill. cv. &#x27;Lady First&#x27;, &#x27;Momotaro&#x27; & &#x27;Minicarol&#x27;) during fruit development. Tomato plants grown in CO2-enriched conditions accumulated more carbohydrates in their fruit and exhibited significantly larger fruits compared to the control (350 ± 50 ppm). The CO2-enriched tomato fruit contained significantly higher concentrations of TSS, fructose and glucose during development. The amount of reducing sugars increased at th...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25422450" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="10297e83b6963f8d9d6e70f0c4bffac5" rel="nofollow" data-download="{"attachment_id":45739252,"asset_id":25422450,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45739252/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="48788222" href="https://iub-bd.academia.edu/shahidulislam">shahidul islam</a><script data-card-contents-for-user="48788222" type="text/json">{"id":48788222,"first_name":"shahidul","last_name":"islam","domain_name":"iub-bd","page_name":"shahidulislam","display_name":"shahidul islam","profile_url":"https://iub-bd.academia.edu/shahidulislam?f_ri=409525","photo":"https://0.academia-photos.com/48788222/12838105/14244262/s65_shahidul.islam.jpg"}</script></span></span></li><li class="js-paper-rank-work_25422450 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="25422450"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 25422450, container: ".js-paper-rank-work_25422450", }); 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Cultivars","created_at":"2016-05-18T03:38:40.656-07:00","url":"https://www.academia.edu/25422450/Elevated_Atmospheric_CO2_Concentration_Enhances_Carbohydrate_Metabolism_in_Developing_Lycopersicon_esculentum_Mill_Cultivars?f_ri=409525","dom_id":"work_25422450","summary":"This study examined the influence of preharvest elevated atmospheric CO2 concentration (850 ± 50 ppm) on the fruit growth, total soluble solids (TSS), fructose, glucose, sucrose, soluble and cell wall-bound acid invertase (AI) (ß-fructofuranoside fructohydrolase, EC 3. 2. 1. 26), sucrose synthase (SuSy) (UDP glucose: D-fructose 2 -glucosyltransferase, EC 2. 4. 1. 13) and sucrose phosphate synthase (SPS) (UDP glucose: D-fructose-6 -phosphate 2 -glucosyltransferase, EC 2. 4. 1. 14) activities of domesticated tomatoes (Lycopersicon esculentum Mill. cv. \u0026#x27;Lady First\u0026#x27;, \u0026#x27;Momotaro\u0026#x27; \u0026 \u0026#x27;Minicarol\u0026#x27;) during fruit development. Tomato plants grown in CO2-enriched conditions accumulated more carbohydrates in their fruit and exhibited significantly larger fruits compared to the control (350 ± 50 ppm). The CO2-enriched tomato fruit contained significantly higher concentrations of TSS, fructose and glucose during development. The amount of reducing sugars increased at th...","downloadable_attachments":[{"id":45739252,"asset_id":25422450,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":48788222,"first_name":"shahidul","last_name":"islam","domain_name":"iub-bd","page_name":"shahidulislam","display_name":"shahidul islam","profile_url":"https://iub-bd.academia.edu/shahidulislam?f_ri=409525","photo":"https://0.academia-photos.com/48788222/12838105/14244262/s65_shahidul.islam.jpg"}],"research_interests":[{"id":4594,"name":"Carbon Dioxide","url":"https://www.academia.edu/Documents/in/Carbon_Dioxide?f_ri=409525","nofollow":false},{"id":14927,"name":"Agriculture 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class="u-tcGrayDark u-fw700" data-has-card-for-user="32782836" href="https://kuleuven.academia.edu/BartLievens">Bart Lievens</a><script data-card-contents-for-user="32782836" type="text/json">{"id":32782836,"first_name":"Bart","last_name":"Lievens","domain_name":"kuleuven","page_name":"BartLievens","display_name":"Bart Lievens","profile_url":"https://kuleuven.academia.edu/BartLievens?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13610858">+2</span><div class="hidden js-additional-users-13610858"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://kuleuven.academia.edu/HansJacquemyn">Hans Jacquemyn</a></span></div><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/MPozo1">M. Pozo</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13610858'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13610858').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_13610858 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13610858"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13610858, container: ".js-paper-rank-work_13610858", }); });</script></li><li class="js-percentile-work_13610858 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 13610858; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_13610858"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_13610858 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="13610858"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 13610858; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=13610858]").text(description); $(".js-view-count-work_13610858").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_13610858").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="13610858"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">9</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="1444" href="https://www.academia.edu/Documents/in/Environmental_microbiology">Environmental microbiology</a>, <script data-card-contents-for-ri="1444" type="text/json">{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9846" href="https://www.academia.edu/Documents/in/Ecology">Ecology</a>, <script data-card-contents-for-ri="9846" type="text/json">{"id":9846,"name":"Ecology","url":"https://www.academia.edu/Documents/in/Ecology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="130822" href="https://www.academia.edu/Documents/in/Flowers">Flowers</a><script data-card-contents-for-ri="130822" type="text/json">{"id":130822,"name":"Flowers","url":"https://www.academia.edu/Documents/in/Flowers?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=13610858]'), work: {"id":13610858,"title":"Microbiology of sugar-rich environments: diversity, ecology and system constraints","created_at":"2015-07-04T02:33:15.763-07:00","url":"https://www.academia.edu/13610858/Microbiology_of_sugar_rich_environments_diversity_ecology_and_system_constraints?f_ri=409525","dom_id":"work_13610858","summary":null,"downloadable_attachments":[{"id":45156365,"asset_id":13610858,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":32782836,"first_name":"Bart","last_name":"Lievens","domain_name":"kuleuven","page_name":"BartLievens","display_name":"Bart Lievens","profile_url":"https://kuleuven.academia.edu/BartLievens?f_ri=409525","photo":"/images/s65_no_pic.png"},{"id":32613950,"first_name":"Hans","last_name":"Jacquemyn","domain_name":"kuleuven","page_name":"HansJacquemyn","display_name":"Hans Jacquemyn","profile_url":"https://kuleuven.academia.edu/HansJacquemyn?f_ri=409525","photo":"/images/s65_no_pic.png"},{"id":32827327,"first_name":"M.","last_name":"Pozo","domain_name":"independent","page_name":"MPozo1","display_name":"M. Pozo","profile_url":"https://independent.academia.edu/MPozo1?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false},{"id":1444,"name":"Environmental microbiology","url":"https://www.academia.edu/Documents/in/Environmental_microbiology?f_ri=409525","nofollow":false},{"id":9846,"name":"Ecology","url":"https://www.academia.edu/Documents/in/Ecology?f_ri=409525","nofollow":false},{"id":130822,"name":"Flowers","url":"https://www.academia.edu/Documents/in/Flowers?f_ri=409525","nofollow":false},{"id":160814,"name":"Fruit","url":"https://www.academia.edu/Documents/in/Fruit?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":447027,"name":"Acinetobacter","url":"https://www.academia.edu/Documents/in/Acinetobacter?f_ri=409525"},{"id":609249,"name":"CARBOHYDRATES","url":"https://www.academia.edu/Documents/in/CARBOHYDRATES?f_ri=409525"},{"id":1208706,"name":"Environment","url":"https://www.academia.edu/Documents/in/Environment?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19085481" data-work_id="19085481" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19085481/Classification_of_the_spoilage_flora_of_raw_and_pasteurized_bovine_milk_with_special_reference_to_Pseudomonas_and_Bacillus">Classification of the spoilage flora of raw and pasteurized bovine milk, with special reference to Pseudomonas and Bacillus</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Eighty-one bacterial strains isolated from refrigerated raw milk, 124 from pasteurized milk and cream stored at 5 degrees C and 7 degrees C, and 19 type and reference strains of Pseudomonas spp. and Bacillus spp. were characterized by... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_19085481" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Eighty-one bacterial strains isolated from refrigerated raw milk, 124 from pasteurized milk and cream stored at 5 degrees C and 7 degrees C, and 19 type and reference strains of Pseudomonas spp. and Bacillus spp. were characterized by numerical phenotypic analysis. Data were processed with simple matching (SSM) and Jaccard (SJ) coefficients, and UPGMA clustering. Fourteen clusters of Gram-negative bacteria were formed at SJ = 79% (SSM = 90%). Raw milk was exclusively spoilt by Gram-negative bacteria, the majority of which were Pseudomonas fluorescens biovar I, Ps. fragi, Ps. lundensis and Ps. fluorescens biovar III. Minor groups in raw milk included Enterobacteriaceae spp. and Acinetobacter spp. Pasteurized milk was spoilt by essentially the same Gram-negative organisms in 65% (5 degrees C) and 50% (7 degrees C) of the cases. The phenotypic characteristics of Gram-negative bacteria are given. Bacillus polymyxa (both temperatures) and B. cereus (only at 7 degrees C) were responsible for 77% of samples spoiled by the Gram-positive organisms. Minor milk spoilage groups included other Bacillus spp. and lactic acid bacteria. All Bacillus spp. grew fermentatively in milk, and most strains denitrified. It is suggested that: (i) industrial recontamination tests of pasteurized milk are directed against Pseudomonas; (ii) milk is stored at 5 degrees C or lower to avoid growth of B. cereus; and (iii) the significance of gas-producing and nitrate/nitrite-reducing Bacillus strains is recognized in cheese production.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19085481" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="436057fdcd328a0976065348fd85b871" rel="nofollow" data-download="{"attachment_id":42111378,"asset_id":19085481,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42111378/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39192867" href="https://lu.academia.edu/G%C3%B6ranMolin">Göran Molin</a><script data-card-contents-for-user="39192867" type="text/json">{"id":39192867,"first_name":"Göran","last_name":"Molin","domain_name":"lu","page_name":"GöranMolin","display_name":"Göran Molin","profile_url":"https://lu.academia.edu/G%C3%B6ranMolin?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19085481 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19085481"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 19085481, container: ".js-paper-rank-work_19085481", }); 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$(".js-view-count[data-work-id=19085481]").text(description); $(".js-view-count-work_19085481").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19085481").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19085481"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="19703" href="https://www.academia.edu/Documents/in/Applied_microbiology">Applied microbiology</a>, <script data-card-contents-for-ri="19703" type="text/json">{"id":19703,"name":"Applied microbiology","url":"https://www.academia.edu/Documents/in/Applied_microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="31632" href="https://www.academia.edu/Documents/in/Food_Microbiology">Food Microbiology</a>, <script data-card-contents-for-ri="31632" type="text/json">{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="112324" href="https://www.academia.edu/Documents/in/Pseudomonas">Pseudomonas</a><script data-card-contents-for-ri="112324" type="text/json">{"id":112324,"name":"Pseudomonas","url":"https://www.academia.edu/Documents/in/Pseudomonas?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19085481]'), work: {"id":19085481,"title":"Classification of the spoilage flora of raw and pasteurized bovine milk, with special reference to Pseudomonas and Bacillus","created_at":"2015-11-27T03:28:43.395-08:00","url":"https://www.academia.edu/19085481/Classification_of_the_spoilage_flora_of_raw_and_pasteurized_bovine_milk_with_special_reference_to_Pseudomonas_and_Bacillus?f_ri=409525","dom_id":"work_19085481","summary":"Eighty-one bacterial strains isolated from refrigerated raw milk, 124 from pasteurized milk and cream stored at 5 degrees C and 7 degrees C, and 19 type and reference strains of Pseudomonas spp. and Bacillus spp. were characterized by numerical phenotypic analysis. Data were processed with simple matching (SSM) and Jaccard (SJ) coefficients, and UPGMA clustering. Fourteen clusters of Gram-negative bacteria were formed at SJ = 79% (SSM = 90%). Raw milk was exclusively spoilt by Gram-negative bacteria, the majority of which were Pseudomonas fluorescens biovar I, Ps. fragi, Ps. lundensis and Ps. fluorescens biovar III. Minor groups in raw milk included Enterobacteriaceae spp. and Acinetobacter spp. Pasteurized milk was spoilt by essentially the same Gram-negative organisms in 65% (5 degrees C) and 50% (7 degrees C) of the cases. The phenotypic characteristics of Gram-negative bacteria are given. Bacillus polymyxa (both temperatures) and B. cereus (only at 7 degrees C) were responsible for 77% of samples spoiled by the Gram-positive organisms. Minor milk spoilage groups included other Bacillus spp. and lactic acid bacteria. All Bacillus spp. grew fermentatively in milk, and most strains denitrified. It is suggested that: (i) industrial recontamination tests of pasteurized milk are directed against Pseudomonas; (ii) milk is stored at 5 degrees C or lower to avoid growth of B. cereus; and (iii) the significance of gas-producing and nitrate/nitrite-reducing Bacillus strains is recognized in cheese production.","downloadable_attachments":[{"id":42111378,"asset_id":19085481,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39192867,"first_name":"Göran","last_name":"Molin","domain_name":"lu","page_name":"GöranMolin","display_name":"Göran Molin","profile_url":"https://lu.academia.edu/G%C3%B6ranMolin?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":19703,"name":"Applied microbiology","url":"https://www.academia.edu/Documents/in/Applied_microbiology?f_ri=409525","nofollow":false},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=409525","nofollow":false},{"id":31632,"name":"Food Microbiology","url":"https://www.academia.edu/Documents/in/Food_Microbiology?f_ri=409525","nofollow":false},{"id":112324,"name":"Pseudomonas","url":"https://www.academia.edu/Documents/in/Pseudomonas?f_ri=409525","nofollow":false},{"id":112332,"name":"Bacillus","url":"https://www.academia.edu/Documents/in/Bacillus?f_ri=409525"},{"id":131237,"name":"Cluster Analysis","url":"https://www.academia.edu/Documents/in/Cluster_Analysis?f_ri=409525"},{"id":213897,"name":"Phenotype","url":"https://www.academia.edu/Documents/in/Phenotype?f_ri=409525"},{"id":237568,"name":"Gram Positive Bacteria","url":"https://www.academia.edu/Documents/in/Gram_Positive_Bacteria?f_ri=409525"},{"id":238368,"name":"Milk","url":"https://www.academia.edu/Documents/in/Milk?f_ri=409525"},{"id":335965,"name":"Gram-negative bacteria","url":"https://www.academia.edu/Documents/in/Gram-negative_bacteria?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_18502648" data-work_id="18502648" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/18502648/The_influence_of_maca_Lepidium_meyenii_on_antioxidant_status_lipid_and_glucose_metabolism_in_rat">The influence of maca (Lepidium meyenii) on antioxidant status, lipid and glucose metabolism in rat</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This work focused on the effect of Maca on lipid, anti-oxidative, and glucose parameters in hereditary hypertriglyceridemic (HHTg) rat. Maca (1%) was administred to rats as a part of a high-sucrose diet (HSD) for 2 weeks. Rosiglitazone... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_18502648" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This work focused on the effect of Maca on lipid, anti-oxidative, and glucose parameters in hereditary hypertriglyceridemic (HHTg) rat. Maca (1%) was administred to rats as a part of a high-sucrose diet (HSD) for 2 weeks. Rosiglitazone (0.02%) was used as a positive control. Maca significantly decreased the levels of VLDL (very low density lipoproteins), LDL (low density lipoproteins), and total cholesterol, and also the level of TAG (triacylglycerols) in the plasma, VLDL, and liver. Maca, as well as rosiglitazone, significantly improved glucose tolerance, as the decrease of AUC (area under the curve) of glucose showed, and lowered levels of glucose in blood. The activity of SOD (superoxide dismutase) in the liver, the GPX (glutathione peroxidase) in the blood, and the level of GSH (glutathione) in liver increased in all cases significantly. Results demonstrate that maca seems to be promising for a positive influence on chronic human diseases (characterized by atherogenous lipoprote...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18502648" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="3a669c35425c3ed0d96ddca5d4065450" rel="nofollow" data-download="{"attachment_id":42164848,"asset_id":18502648,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42164848/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="4667561" href="https://independent.academia.edu/Vil%C3%ADm%C5%A0im%C3%A1nek">Vilím Šimánek</a><script data-card-contents-for-user="4667561" type="text/json">{"id":4667561,"first_name":"Vilím","last_name":"Šimánek","domain_name":"independent","page_name":"VilímŠimánek","display_name":"Vilím Šimánek","profile_url":"https://independent.academia.edu/Vil%C3%ADm%C5%A0im%C3%A1nek?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_18502648 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="18502648"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 18502648, container: ".js-paper-rank-work_18502648", }); 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$(".js-view-count[data-work-id=18502648]").text(description); $(".js-view-count-work_18502648").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_18502648").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="18502648"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="51711" href="https://www.academia.edu/Documents/in/Antioxidants">Antioxidants</a>, <script data-card-contents-for-ri="51711" type="text/json">{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71289" href="https://www.academia.edu/Documents/in/Glucose">Glucose</a>, <script data-card-contents-for-ri="71289" type="text/json">{"id":71289,"name":"Glucose","url":"https://www.academia.edu/Documents/in/Glucose?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71437" href="https://www.academia.edu/Documents/in/Liver">Liver</a>, <script data-card-contents-for-ri="71437" type="text/json">{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="90514" href="https://www.academia.edu/Documents/in/Cholesterol">Cholesterol</a><script data-card-contents-for-ri="90514" type="text/json">{"id":90514,"name":"Cholesterol","url":"https://www.academia.edu/Documents/in/Cholesterol?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=18502648]'), work: {"id":18502648,"title":"The influence of maca (Lepidium meyenii) on antioxidant status, lipid and glucose metabolism in rat","created_at":"2015-11-17T05:37:44.916-08:00","url":"https://www.academia.edu/18502648/The_influence_of_maca_Lepidium_meyenii_on_antioxidant_status_lipid_and_glucose_metabolism_in_rat?f_ri=409525","dom_id":"work_18502648","summary":"This work focused on the effect of Maca on lipid, anti-oxidative, and glucose parameters in hereditary hypertriglyceridemic (HHTg) rat. Maca (1%) was administred to rats as a part of a high-sucrose diet (HSD) for 2 weeks. Rosiglitazone (0.02%) was used as a positive control. Maca significantly decreased the levels of VLDL (very low density lipoproteins), LDL (low density lipoproteins), and total cholesterol, and also the level of TAG (triacylglycerols) in the plasma, VLDL, and liver. Maca, as well as rosiglitazone, significantly improved glucose tolerance, as the decrease of AUC (area under the curve) of glucose showed, and lowered levels of glucose in blood. The activity of SOD (superoxide dismutase) in the liver, the GPX (glutathione peroxidase) in the blood, and the level of GSH (glutathione) in liver increased in all cases significantly. Results demonstrate that maca seems to be promising for a positive influence on chronic human diseases (characterized by atherogenous lipoprote...","downloadable_attachments":[{"id":42164848,"asset_id":18502648,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4667561,"first_name":"Vilím","last_name":"Šimánek","domain_name":"independent","page_name":"VilímŠimánek","display_name":"Vilím Šimánek","profile_url":"https://independent.academia.edu/Vil%C3%ADm%C5%A0im%C3%A1nek?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":51711,"name":"Antioxidants","url":"https://www.academia.edu/Documents/in/Antioxidants?f_ri=409525","nofollow":false},{"id":71289,"name":"Glucose","url":"https://www.academia.edu/Documents/in/Glucose?f_ri=409525","nofollow":false},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=409525","nofollow":false},{"id":90514,"name":"Cholesterol","url":"https://www.academia.edu/Documents/in/Cholesterol?f_ri=409525","nofollow":false},{"id":118450,"name":"Glutathione","url":"https://www.academia.edu/Documents/in/Glutathione?f_ri=409525"},{"id":141395,"name":"Glutathione Peroxidase","url":"https://www.academia.edu/Documents/in/Glutathione_Peroxidase?f_ri=409525"},{"id":184609,"name":"Lipid metabolism","url":"https://www.academia.edu/Documents/in/Lipid_metabolism?f_ri=409525"},{"id":213343,"name":"Superoxide Dismutase","url":"https://www.academia.edu/Documents/in/Superoxide_Dismutase?f_ri=409525"},{"id":227299,"name":"Triglycerides","url":"https://www.academia.edu/Documents/in/Triglycerides?f_ri=409525"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=409525"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":564879,"name":"Wistar Rats","url":"https://www.academia.edu/Documents/in/Wistar_Rats?f_ri=409525"},{"id":1309706,"name":"Area Under Curve","url":"https://www.academia.edu/Documents/in/Area_Under_Curve?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_32166137" data-work_id="32166137" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/32166137/Regulation_of_crystal_protein_biosynthesis_by_Bacillus_thuringiensis_II_Effects_of_carbon_and_nitrogen_sources">Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/32166137" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a1e52b6b7e757070946a7a1c95031344" rel="nofollow" data-download="{"attachment_id":52402496,"asset_id":32166137,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/52402496/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="35116316" href="https://independent.academia.edu/BIcgen">Bulent Icgen</a><script data-card-contents-for-user="35116316" type="text/json">{"id":35116316,"first_name":"Bulent","last_name":"Icgen","domain_name":"independent","page_name":"BIcgen","display_name":"Bulent Icgen","profile_url":"https://independent.academia.edu/BIcgen?f_ri=409525","photo":"https://0.academia-photos.com/35116316/16180463/150818983/s65_bulent.icgen.jpeg"}</script></span></span></li><li class="js-paper-rank-work_32166137 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="32166137"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 32166137, container: ".js-paper-rank-work_32166137", }); 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$(".js-view-count[data-work-id=32166137]").text(description); $(".js-view-count-work_32166137").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_32166137").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="32166137"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5303" href="https://www.academia.edu/Documents/in/Carbon">Carbon</a>, <script data-card-contents-for-ri="5303" type="text/json">{"id":5303,"name":"Carbon","url":"https://www.academia.edu/Documents/in/Carbon?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6947" href="https://www.academia.edu/Documents/in/Medical_Microbiology">Medical Microbiology</a>, <script data-card-contents-for-ri="6947" type="text/json">{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="11035" href="https://www.academia.edu/Documents/in/Regulation">Regulation</a><script data-card-contents-for-ri="11035" type="text/json">{"id":11035,"name":"Regulation","url":"https://www.academia.edu/Documents/in/Regulation?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=32166137]'), work: {"id":32166137,"title":"Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources","created_at":"2017-03-31T07:31:43.957-07:00","url":"https://www.academia.edu/32166137/Regulation_of_crystal_protein_biosynthesis_by_Bacillus_thuringiensis_II_Effects_of_carbon_and_nitrogen_sources?f_ri=409525","dom_id":"work_32166137","summary":null,"downloadable_attachments":[{"id":52402496,"asset_id":32166137,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":35116316,"first_name":"Bulent","last_name":"Icgen","domain_name":"independent","page_name":"BIcgen","display_name":"Bulent Icgen","profile_url":"https://independent.academia.edu/BIcgen?f_ri=409525","photo":"https://0.academia-photos.com/35116316/16180463/150818983/s65_bulent.icgen.jpeg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=409525","nofollow":false},{"id":5303,"name":"Carbon","url":"https://www.academia.edu/Documents/in/Carbon?f_ri=409525","nofollow":false},{"id":6947,"name":"Medical Microbiology","url":"https://www.academia.edu/Documents/in/Medical_Microbiology?f_ri=409525","nofollow":false},{"id":11035,"name":"Regulation","url":"https://www.academia.edu/Documents/in/Regulation?f_ri=409525","nofollow":false},{"id":38139,"name":"Protein synthesis","url":"https://www.academia.edu/Documents/in/Protein_synthesis?f_ri=409525"},{"id":52972,"name":"Insecticides","url":"https://www.academia.edu/Documents/in/Insecticides?f_ri=409525"},{"id":67768,"name":"Bacillus thuringiensis","url":"https://www.academia.edu/Documents/in/Bacillus_thuringiensis?f_ri=409525"},{"id":70902,"name":"Magnesium","url":"https://www.academia.edu/Documents/in/Magnesium?f_ri=409525"},{"id":80692,"name":"Copper","url":"https://www.academia.edu/Documents/in/Copper?f_ri=409525"},{"id":81067,"name":"Bacterial Toxins","url":"https://www.academia.edu/Documents/in/Bacterial_Toxins?f_ri=409525"},{"id":84278,"name":"Ph","url":"https://www.academia.edu/Documents/in/Ph?f_ri=409525"},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=409525"},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen?f_ri=409525"},{"id":406914,"name":"Biopesticide","url":"https://www.academia.edu/Documents/in/Biopesticide?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":541785,"name":"Phosphates","url":"https://www.academia.edu/Documents/in/Phosphates?f_ri=409525"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=409525"},{"id":1451915,"name":"Endotoxins","url":"https://www.academia.edu/Documents/in/Endotoxins?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_14989617 coauthored" data-work_id="14989617" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14989617/Purification_of_a_secreted_lectin_from_Andrias_davidianus_skin_and_its_antibacterial_activity">Purification of a secreted lectin from Andrias davidianus skin and its antibacterial activity</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A lectin secreted from Andrias davidianus skin (ADL) was purified by affinity chromatography on porcine stomach mucin (type III) (PSM)-crosslinked albumin, followed by gel filtration on Sephadex G-100 and HPLC on TSK gel G3000PWXL. The... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_14989617" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A lectin secreted from Andrias davidianus skin (ADL) was purified by affinity chromatography on porcine stomach mucin (type III) (PSM)-crosslinked albumin, followed by gel filtration on Sephadex G-100 and HPLC on TSK gel G3000PWXL. The purified lectin was found to be a dimeric protein, as revealed by SDS-PAGE and MALDI-TOF analysis. SDS-PAGE showed that the ADL protein had a molecular mass of 17 kDa. ADL produced an 8.5 kDa band when examined using SDS-PAGE under reducing conditions. ADL agglutinated native and trypsinized human B erythrocytes. The hemagglutination activity was inhibited by glycoproteins, such as PSM and asialo-PSM, but not by any of the monosaccharides tested. The activity was stable between 4 °C and 50 °C. Significant ADL activity was observed between pH 4–5. The lectin reaction did not depend on the presence of the divalent cation Ca2+ or Mg2+. The N-terminal ADL sequence was determined to be VGYTVGATPM. The lectin exhibited antibacterial activity, involving grow...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14989617" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="273304fb3f2ae8a45e81a2111b352779" rel="nofollow" data-download="{"attachment_id":43671431,"asset_id":14989617,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43671431/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="2132868" href="https://dvo.academia.edu/PavelLukyanov">Pavel Lukyanov</a><script data-card-contents-for-user="2132868" type="text/json">{"id":2132868,"first_name":"Pavel","last_name":"Lukyanov","domain_name":"dvo","page_name":"PavelLukyanov","display_name":"Pavel Lukyanov","profile_url":"https://dvo.academia.edu/PavelLukyanov?f_ri=409525","photo":"https://0.academia-photos.com/2132868/17921725/17935893/s65_pavel.lukyanov.jpg"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-14989617">+1</span><div class="hidden js-additional-users-14989617"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/MinQu">Min Qu</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-14989617'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-14989617').html(); 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container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_14989617 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="14989617"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 14989617; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=14989617]").text(description); $(".js-view-count-work_14989617").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_14989617").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="14989617"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="49123" href="https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae">Saccharomyces cerevisiae</a>, <script data-card-contents-for-ri="49123" type="text/json">{"id":49123,"name":"Saccharomyces cerevisiae","url":"https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="58054" href="https://www.academia.edu/Documents/in/Environmental_Sciences">Environmental Sciences</a>, <script data-card-contents-for-ri="58054" type="text/json">{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="113903" href="https://www.academia.edu/Documents/in/Bacteria">Bacteria</a><script data-card-contents-for-ri="113903" type="text/json">{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=14989617]'), work: {"id":14989617,"title":"Purification of a secreted lectin from Andrias davidianus skin and its antibacterial activity","created_at":"2015-08-17T11:25:50.015-07:00","url":"https://www.academia.edu/14989617/Purification_of_a_secreted_lectin_from_Andrias_davidianus_skin_and_its_antibacterial_activity?f_ri=409525","dom_id":"work_14989617","summary":"A lectin secreted from Andrias davidianus skin (ADL) was purified by affinity chromatography on porcine stomach mucin (type III) (PSM)-crosslinked albumin, followed by gel filtration on Sephadex G-100 and HPLC on TSK gel G3000PWXL. The purified lectin was found to be a dimeric protein, as revealed by SDS-PAGE and MALDI-TOF analysis. SDS-PAGE showed that the ADL protein had a molecular mass of 17 kDa. ADL produced an 8.5 kDa band when examined using SDS-PAGE under reducing conditions. ADL agglutinated native and trypsinized human B erythrocytes. The hemagglutination activity was inhibited by glycoproteins, such as PSM and asialo-PSM, but not by any of the monosaccharides tested. The activity was stable between 4 °C and 50 °C. Significant ADL activity was observed between pH 4–5. The lectin reaction did not depend on the presence of the divalent cation Ca2+ or Mg2+. The N-terminal ADL sequence was determined to be VGYTVGATPM. The lectin exhibited antibacterial activity, involving grow...","downloadable_attachments":[{"id":43671431,"asset_id":14989617,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":2132868,"first_name":"Pavel","last_name":"Lukyanov","domain_name":"dvo","page_name":"PavelLukyanov","display_name":"Pavel Lukyanov","profile_url":"https://dvo.academia.edu/PavelLukyanov?f_ri=409525","photo":"https://0.academia-photos.com/2132868/17921725/17935893/s65_pavel.lukyanov.jpg"},{"id":33985377,"first_name":"Min","last_name":"Qu","domain_name":"independent","page_name":"MinQu","display_name":"Min Qu","profile_url":"https://independent.academia.edu/MinQu?f_ri=409525","photo":"https://0.academia-photos.com/33985377/146832654/136378250/s65_min.qu.png"}],"research_interests":[{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525","nofollow":false},{"id":49123,"name":"Saccharomyces cerevisiae","url":"https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae?f_ri=409525","nofollow":false},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences?f_ri=409525","nofollow":false},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria?f_ri=409525","nofollow":false},{"id":138998,"name":"Lectins","url":"https://www.academia.edu/Documents/in/Lectins?f_ri=409525"},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin?f_ri=409525"},{"id":335984,"name":"Anti-Bacterial Agents","url":"https://www.academia.edu/Documents/in/Anti-Bacterial_Agents?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":552209,"name":"Antifungal Agents","url":"https://www.academia.edu/Documents/in/Antifungal_Agents?f_ri=409525"},{"id":707063,"name":"Urodela","url":"https://www.academia.edu/Documents/in/Urodela?f_ri=409525"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22021661" data-work_id="22021661" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22021661/Fat_adaptation_in_well_trained_athletes_effects_on_cell_metabolism">Fat adaptation in well-trained athletes: effects on cell metabolism</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22021661" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1db6d76bb941d10361e2a9bdbf1174bc" rel="nofollow" data-download="{"attachment_id":42715365,"asset_id":22021661,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42715365/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="43286735" href="https://independent.academia.edu/LouiseBurke1">Louise Burke</a><script data-card-contents-for-user="43286735" type="text/json">{"id":43286735,"first_name":"Louise","last_name":"Burke","domain_name":"independent","page_name":"LouiseBurke1","display_name":"Louise Burke","profile_url":"https://independent.academia.edu/LouiseBurke1?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_22021661 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="22021661"><i class="u-m1x fa 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view-count u-mr2x" data-work-id="22021661"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 22021661; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=22021661]").text(description); $(".js-view-count-work_22021661").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_22021661").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="22021661"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="3770" href="https://www.academia.edu/Documents/in/Metabolism">Metabolism</a>, <script data-card-contents-for-ri="3770" type="text/json">{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4228" href="https://www.academia.edu/Documents/in/Skeletal_muscle_biology">Skeletal muscle biology</a>, <script data-card-contents-for-ri="4228" type="text/json">{"id":4228,"name":"Skeletal muscle biology","url":"https://www.academia.edu/Documents/in/Skeletal_muscle_biology?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5677" href="https://www.academia.edu/Documents/in/Adaptation">Adaptation</a>, <script data-card-contents-for-ri="5677" type="text/json">{"id":5677,"name":"Adaptation","url":"https://www.academia.edu/Documents/in/Adaptation?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9478" href="https://www.academia.edu/Documents/in/Diet">Diet</a><script data-card-contents-for-ri="9478" type="text/json">{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22021661]'), work: {"id":22021661,"title":"Fat adaptation in well-trained athletes: effects on cell metabolism","created_at":"2016-02-15T16:49:52.832-08:00","url":"https://www.academia.edu/22021661/Fat_adaptation_in_well_trained_athletes_effects_on_cell_metabolism?f_ri=409525","dom_id":"work_22021661","summary":null,"downloadable_attachments":[{"id":42715365,"asset_id":22021661,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":43286735,"first_name":"Louise","last_name":"Burke","domain_name":"independent","page_name":"LouiseBurke1","display_name":"Louise Burke","profile_url":"https://independent.academia.edu/LouiseBurke1?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":3770,"name":"Metabolism","url":"https://www.academia.edu/Documents/in/Metabolism?f_ri=409525","nofollow":false},{"id":4228,"name":"Skeletal muscle biology","url":"https://www.academia.edu/Documents/in/Skeletal_muscle_biology?f_ri=409525","nofollow":false},{"id":5677,"name":"Adaptation","url":"https://www.academia.edu/Documents/in/Adaptation?f_ri=409525","nofollow":false},{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=409525","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=409525"},{"id":50456,"name":"Sports","url":"https://www.academia.edu/Documents/in/Sports?f_ri=409525"},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids?f_ri=409525"},{"id":135185,"name":"Exercise","url":"https://www.academia.edu/Documents/in/Exercise?f_ri=409525"},{"id":184609,"name":"Lipid metabolism","url":"https://www.academia.edu/Documents/in/Lipid_metabolism?f_ri=409525"},{"id":219568,"name":"Athletes","url":"https://www.academia.edu/Documents/in/Athletes?f_ri=409525"},{"id":341038,"name":"AMP","url":"https://www.academia.edu/Documents/in/AMP?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":490954,"name":"Fat","url":"https://www.academia.edu/Documents/in/Fat?f_ri=409525"},{"id":546429,"name":"Dietary Carbohydrates","url":"https://www.academia.edu/Documents/in/Dietary_Carbohydrates?f_ri=409525"},{"id":1232429,"name":"Glycogen","url":"https://www.academia.edu/Documents/in/Glycogen?f_ri=409525"},{"id":1256747,"name":"Oxidation-Reduction","url":"https://www.academia.edu/Documents/in/Oxidation-Reduction?f_ri=409525"},{"id":2183225,"name":"Physical Endurance","url":"https://www.academia.edu/Documents/in/Physical_Endurance?f_ri=409525"},{"id":2348526,"name":"Dietary fats","url":"https://www.academia.edu/Documents/in/Dietary_fats?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_23481990" data-work_id="23481990" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/23481990/Glycemic_index_of_foods_a_physiological_basis_for_carbohydrate_exchange">Glycemic index of foods: a physiological basis for carbohydrate exchange</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The determine the effect of different foods on the blood glucose, 62 commonly eaten foods and sugars were fed individually to groups of 5 to 10 healthy fasting volunteers. Blood glucose levels were measured over 2 h, and expressed as a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_23481990" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The determine the effect of different foods on the blood glucose, 62 commonly eaten foods and sugars were fed individually to groups of 5 to 10 healthy fasting volunteers. Blood glucose levels were measured over 2 h, and expressed as a percentage of the area under the glucose response curve when the same amount of carbohydrate was taken as glucose. The largest rises were seen with vegetables (70 +/- 5%), followed by breakfast cereals (65 +/- 5%), cereals and biscuits (60 +/- 3%), fruit (50 +/- 5%), dairy products (35 +/- 1%), and dried legumes (31 +/- 3%). A significant negative relationship was seen between fat (p less than 0.01) and protein (p less than 0.001) and postprandial glucose rise but not with fiber or sugar content.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/23481990" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b1a663fc958639f115c529795e5c48ba" rel="nofollow" data-download="{"attachment_id":43918809,"asset_id":23481990,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43918809/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="45463897" href="https://independent.academia.edu/JenkinsAlexandra">Alexandra Jenkins</a><script data-card-contents-for-user="45463897" type="text/json">{"id":45463897,"first_name":"Alexandra","last_name":"Jenkins","domain_name":"independent","page_name":"JenkinsAlexandra","display_name":"Alexandra Jenkins","profile_url":"https://independent.academia.edu/JenkinsAlexandra?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_23481990 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="23481990"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 23481990, container: ".js-paper-rank-work_23481990", }); 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$(".js-view-count[data-work-id=23481990]").text(description); $(".js-view-count-work_23481990").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_23481990").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="23481990"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="9478" href="https://www.academia.edu/Documents/in/Diet">Diet</a>, <script data-card-contents-for-ri="9478" type="text/json">{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="39719" href="https://www.academia.edu/Documents/in/Food_Analysis">Food Analysis</a>, <script data-card-contents-for-ri="39719" type="text/json">{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71300" href="https://www.academia.edu/Documents/in/Blood_Glucose">Blood Glucose</a><script data-card-contents-for-ri="71300" type="text/json">{"id":71300,"name":"Blood Glucose","url":"https://www.academia.edu/Documents/in/Blood_Glucose?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=23481990]'), work: {"id":23481990,"title":"Glycemic index of foods: a physiological basis for carbohydrate exchange","created_at":"2016-03-20T06:07:16.697-07:00","url":"https://www.academia.edu/23481990/Glycemic_index_of_foods_a_physiological_basis_for_carbohydrate_exchange?f_ri=409525","dom_id":"work_23481990","summary":"The determine the effect of different foods on the blood glucose, 62 commonly eaten foods and sugars were fed individually to groups of 5 to 10 healthy fasting volunteers. Blood glucose levels were measured over 2 h, and expressed as a percentage of the area under the glucose response curve when the same amount of carbohydrate was taken as glucose. The largest rises were seen with vegetables (70 +/- 5%), followed by breakfast cereals (65 +/- 5%), cereals and biscuits (60 +/- 3%), fruit (50 +/- 5%), dairy products (35 +/- 1%), and dried legumes (31 +/- 3%). A significant negative relationship was seen between fat (p less than 0.01) and protein (p less than 0.001) and postprandial glucose rise but not with fiber or sugar content.","downloadable_attachments":[{"id":43918809,"asset_id":23481990,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":45463897,"first_name":"Alexandra","last_name":"Jenkins","domain_name":"independent","page_name":"JenkinsAlexandra","display_name":"Alexandra Jenkins","profile_url":"https://independent.academia.edu/JenkinsAlexandra?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=409525","nofollow":false},{"id":9478,"name":"Diet","url":"https://www.academia.edu/Documents/in/Diet?f_ri=409525","nofollow":false},{"id":39719,"name":"Food Analysis","url":"https://www.academia.edu/Documents/in/Food_Analysis?f_ri=409525","nofollow":false},{"id":71300,"name":"Blood Glucose","url":"https://www.academia.edu/Documents/in/Blood_Glucose?f_ri=409525","nofollow":false},{"id":151448,"name":"American","url":"https://www.academia.edu/Documents/in/American?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":452474,"name":"Dietary fiber","url":"https://www.academia.edu/Documents/in/Dietary_fiber?f_ri=409525"},{"id":546429,"name":"Dietary Carbohydrates","url":"https://www.academia.edu/Documents/in/Dietary_Carbohydrates?f_ri=409525"},{"id":559553,"name":"Dairy Products","url":"https://www.academia.edu/Documents/in/Dairy_Products?f_ri=409525"},{"id":2348526,"name":"Dietary fats","url":"https://www.academia.edu/Documents/in/Dietary_fats?f_ri=409525"},{"id":2423707,"name":"Diabetic Diet","url":"https://www.academia.edu/Documents/in/Diabetic_Diet?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22125615 coauthored" data-work_id="22125615" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22125615/Insulin_effects_in_muscle_and_adipose_tissue">Insulin effects in muscle and adipose tissue</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22125615" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0f92b2690729d5f44e42893c1447647c" rel="nofollow" data-download="{"attachment_id":42793676,"asset_id":22125615,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/42793676/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42040749" href="https://independent.academia.edu/EiriniMaratou">Eirini Maratou</a><script data-card-contents-for-user="42040749" type="text/json">{"id":42040749,"first_name":"Eirini","last_name":"Maratou","domain_name":"independent","page_name":"EiriniMaratou","display_name":"Eirini Maratou","profile_url":"https://independent.academia.edu/EiriniMaratou?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-22125615">+1</span><div class="hidden js-additional-users-22125615"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/PanayotaMitrou">Panayota Mitrou</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-22125615'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-22125615').html(); } } new HoverPopover(popoverSettings); })();</script></li><li class="js-paper-rank-work_22125615 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="22125615"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 22125615, container: ".js-paper-rank-work_22125615", }); });</script></li><li class="js-percentile-work_22125615 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 22125615; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_22125615"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_22125615 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="22125615"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 22125615; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=22125615]").text(description); $(".js-view-count-work_22125615").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_22125615").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="22125615"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4581" href="https://www.academia.edu/Documents/in/Diabetes">Diabetes</a>, <script data-card-contents-for-ri="4581" type="text/json">{"id":4581,"name":"Diabetes","url":"https://www.academia.edu/Documents/in/Diabetes?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="38139" href="https://www.academia.edu/Documents/in/Protein_synthesis">Protein synthesis</a>, <script data-card-contents-for-ri="38139" type="text/json">{"id":38139,"name":"Protein synthesis","url":"https://www.academia.edu/Documents/in/Protein_synthesis?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="52489" href="https://www.academia.edu/Documents/in/Adipose_tissue">Adipose tissue</a>, <script data-card-contents-for-ri="52489" type="text/json">{"id":52489,"name":"Adipose tissue","url":"https://www.academia.edu/Documents/in/Adipose_tissue?f_ri=409525","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71400" href="https://www.academia.edu/Documents/in/Insulin">Insulin</a><script data-card-contents-for-ri="71400" type="text/json">{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin?f_ri=409525","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22125615]'), work: {"id":22125615,"title":"Insulin effects in muscle and adipose tissue","created_at":"2016-02-18T01:27:59.555-08:00","url":"https://www.academia.edu/22125615/Insulin_effects_in_muscle_and_adipose_tissue?f_ri=409525","dom_id":"work_22125615","summary":null,"downloadable_attachments":[{"id":42793676,"asset_id":22125615,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":42040749,"first_name":"Eirini","last_name":"Maratou","domain_name":"independent","page_name":"EiriniMaratou","display_name":"Eirini Maratou","profile_url":"https://independent.academia.edu/EiriniMaratou?f_ri=409525","photo":"/images/s65_no_pic.png"},{"id":43452664,"first_name":"Panayota","last_name":"Mitrou","domain_name":"independent","page_name":"PanayotaMitrou","display_name":"Panayota Mitrou","profile_url":"https://independent.academia.edu/PanayotaMitrou?f_ri=409525","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4581,"name":"Diabetes","url":"https://www.academia.edu/Documents/in/Diabetes?f_ri=409525","nofollow":false},{"id":38139,"name":"Protein synthesis","url":"https://www.academia.edu/Documents/in/Protein_synthesis?f_ri=409525","nofollow":false},{"id":52489,"name":"Adipose tissue","url":"https://www.academia.edu/Documents/in/Adipose_tissue?f_ri=409525","nofollow":false},{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin?f_ri=409525","nofollow":false},{"id":71469,"name":"Muscle","url":"https://www.academia.edu/Documents/in/Muscle?f_ri=409525"},{"id":127955,"name":"Fatty Acid Oxidation","url":"https://www.academia.edu/Documents/in/Fatty_Acid_Oxidation?f_ri=409525"},{"id":134095,"name":"Muscles","url":"https://www.academia.edu/Documents/in/Muscles?f_ri=409525"},{"id":184609,"name":"Lipid metabolism","url":"https://www.academia.edu/Documents/in/Lipid_metabolism?f_ri=409525"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=409525"},{"id":306952,"name":"Glucose Transport","url":"https://www.academia.edu/Documents/in/Glucose_Transport?f_ri=409525"},{"id":311112,"name":"Protein Metabolism","url":"https://www.academia.edu/Documents/in/Protein_Metabolism?f_ri=409525"},{"id":409525,"name":"Carbohydrate metabolism","url":"https://www.academia.edu/Documents/in/Carbohydrate_metabolism?f_ri=409525"},{"id":486713,"name":"Fatty Acid","url":"https://www.academia.edu/Documents/in/Fatty_Acid?f_ri=409525"},{"id":500737,"name":"Protein Degradation","url":"https://www.academia.edu/Documents/in/Protein_Degradation?f_ri=409525"},{"id":585573,"name":"Amino Acid Profile","url":"https://www.academia.edu/Documents/in/Amino_Acid_Profile?f_ri=409525"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_70484078" data-work_id="70484078" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/70484078/Molecular_Cloning_Carbohydrate_Specificity_and_the_Crystal_Structure_of_Two_Sclerotium_rolfsii_Lectin_Variants">Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/70484078" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0f949849c2ecd61a1a40d025c3f34432" rel="nofollow" data-download="{"attachment_id":80219410,"asset_id":70484078,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/80219410/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="160900481" href="https://independent.academia.edu/DrVishwanathChachadi">Dr. Vishwanath Chachadi</a><script data-card-contents-for-user="160900481" type="text/json">{"id":160900481,"first_name":"Dr. Vishwanath","last_name":"Chachadi","domain_name":"independent","page_name":"DrVishwanathChachadi","display_name":"Dr. Vishwanath Chachadi","profile_url":"https://independent.academia.edu/DrVishwanathChachadi?f_ri=409525","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_70484078 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="70484078"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 70484078, container: ".js-paper-rank-work_70484078", }); });</script></li><li class="js-percentile-work_70484078 InlineList-item InlineList-item--bordered hidden 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class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_5983180" data-work_id="5983180" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/5983180/Study_of_the_Adhesion_of_Bifidobacterium_bifidum_MIMBb75_to_Human_Intestinal_Cell_Lines">Study of the Adhesion of Bifidobacterium bifidum MIMBb75 to Human Intestinal Cell Lines</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The aim of this study was to investigate the adhesive phenotype of the human intestinal isolate Bifidobacterium bifidum MIMBb75 to human colon carcinoma cell lines. We have previously shown that the adhesion of this strain to Caco-2 cells... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_5983180" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The aim of this study was to investigate the adhesive phenotype of the human intestinal isolate Bifidobacterium bifidum MIMBb75 to human colon carcinoma cell lines. We have previously shown that the adhesion of this strain to Caco-2 cells is mediated by an abundant surface lipoprotein named BopA. In this study, we found that this strain adheres to Caco-2 and HT-29 cells, and that its adhesion strongly depends on the environmental conditions, including the presence of sugars and bile salts and the pH. Considerably more adhesion to a Caco-2 monolayer occurred in the presence of fucose and mannose and less when MIMBb75 grew in Oxgall bile salts compared to standard environmental conditions. In particular, growth in Oxgall bile salts reduced the adhesion ability of MIMBb75 and modified the SDS-PAGE profile of the cell wall associated proteins of the strain. The pH markedly affected both adhesion to Caco-2 and bacterial autoaggregation. Finally, experiments with sodium metaperiodate suggested that not only proteinaceous determinants are involved in the adhesion process of B. bifidum. In conclusion, it seems that the colonization strategy of this bacterium can be influenced by factors varying along the gastrointestinal tract, such as the presence of specific sugars and bile salts and the pH, possibly limiting the adhesion of B. bifidum to only restricted distal sites of the gut.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/5983180" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="6c64c718eccc3fde1143091a314611ea" rel="nofollow" data-download="{"attachment_id":49067711,"asset_id":5983180,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49067711/download_file?st=MTczMjc0NDE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="1156289" href="https://unimi.academia.edu/SimoneGuglielmetti">Simone Guglielmetti</a><script data-card-contents-for-user="1156289" type="text/json">{"id":1156289,"first_name":"Simone","last_name":"Guglielmetti","domain_name":"unimi","page_name":"SimoneGuglielmetti","display_name":"Simone Guglielmetti","profile_url":"https://unimi.academia.edu/SimoneGuglielmetti?f_ri=409525","photo":"https://0.academia-photos.com/1156289/408418/500670/s65_simone.guglielmetti.jpg"}</script></span></span></li><li class="js-paper-rank-work_5983180 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="5983180"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 5983180, container: ".js-paper-rank-work_5983180", }); 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We have previously shown that the adhesion of this strain to Caco-2 cells is mediated by an abundant surface lipoprotein named BopA. In this study, we found that this strain adheres to Caco-2 and HT-29 cells, and that its adhesion strongly depends on the environmental conditions, including the presence of sugars and bile salts and the pH. Considerably more adhesion to a Caco-2 monolayer occurred in the presence of fucose and mannose and less when MIMBb75 grew in Oxgall bile salts compared to standard environmental conditions. In particular, growth in Oxgall bile salts reduced the adhesion ability of MIMBb75 and modified the SDS-PAGE profile of the cell wall associated proteins of the strain. The pH markedly affected both adhesion to Caco-2 and bacterial autoaggregation. Finally, experiments with sodium metaperiodate suggested that not only proteinaceous determinants are involved in the adhesion process of B. bifidum. 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