Next Generation Protein Sequencing Technology

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align-middle row-reverse"> <div class="column"> </div> <div class="column"> <h2> </h2> <p> <p><strong>The next frontier of discovery aims to revolutionize understanding of the human proteome and explore function at the cellular level.</strong> Next-Generation Protein Sequencing™ (NGPS) reveals insights to critical questions:</p> <ul> <li>Is a specific protein present?</li> <li>Which proteins are important to health and disease?</li> <li>Why are they important?</li> <li>How do proteins change with disease and in response to therapy?</li> </ul> <p>With Next-Generation Protein Sequencing, you can answer these critical questions quickly, right in your own lab.</p> </p> </div> </div> </section> <section class="video-player" data-block_3436d7f06beb0b3c532b54da23e85737> <div class="row align-center"> <div class="column small-12 xlarge-11 text-center"> <h2 class="underline">Learn About Our Technology</h2> <div class="video-wrapper"> <div class="responsive-embed widescreen"> <video src="https://www.quantum-si.com/wp-content/uploads/QuantumSi_Final.mp4" poster=""></video> <div class="play-button"> <span class="material-symbols-outlined">play_arrow</span> </div> </div> <div class="video-controls row large-"> <div class="column small-12"> <div class="slider time-slider" data-slider data-initial-start="0" data-end="100" step="0"> <span class="slider-handle time-slider-handle" data-slider-handle role="slider" tabindex="1"></span> <span class="slider-fill time-slider-fill" data-slider-fill></span> <input type="hidden" class="time-slider-value"> </div> </div> <div class="column"> <div class="row"> <div class="column shrink icons"> <span class="playpause" data-state="play"> <span class="play-icon material-symbols-outlined">play_arrow</span> <span class="pause-icon material-symbols-outlined">pause</span> </span> <span class="stop"></span> <span class="mute" data-state="mute"> <span class="unmute-icon material-symbols-outlined">volume_up</span> <span class="mute-icon material-symbols-outlined">volume_off</span> </span> </div> <div class="column shrink icons show-for-large"> <div class="slider volume-slider" data-slider data-initial-start="50" data-end="100"> <span class="slider-handle volume-slider-handle" data-slider-handle role="slider" tabindex="1"></span> <span class="slider-fill volume-slider-fill" data-slider-fill></span> <input type="hidden" class="volume-slider-value"> </div> </div> <div class="column time"> <span class="time-current">0:00</span> / <span class="time-total">0:00</span> </div> </div> </div> <div class="column text-right shrink"> <span class="pip material-symbols-outlined">picture_in_picture_alt</span> <span class="fs material-symbols-outlined">fullscreen</span> </div> </div> </div> <p> </p> </div> </div> </section> <div class="wp-block-columns is-layout-flex wp-container-core-columns-is-layout-1 wp-block-columns-is-layout-flex"> <div class="wp-block-column how-it-work-section is-layout-flow wp-block-column-is-layout-flow"><section class="text-columns" data-block_63f853871fa63ffc3116559e88222868 id="text-columns-how-it-works"> <div class="row column text-center"> <h2 class="underline">How It Works</h2> </div> <div class="image row column"> <img decoding="async" src="https://www.quantum-si.com/wp-content/uploads/img_seq_workflow.svg" alt="" loading="lazy"> </div> <div class="row align-spaced small-up-1 medium-up-3 large-up-3 text-left" data-equalizer data-equalize-on="medium"> <div class="column"> <div data-equalizer-watch> <div class="item-image"> <img decoding="async" src="https://www.quantum-si.com/wp-content/uploads/img_workflow_prepare.svg" alt="" loading="lazy"> </div> <h3>Prepare</h3> <p> <p>Library Preparation and Sequencing Kits contain everything you need, including reagents to digest and functionalize proteins and immobilize peptides on semiconductor chips, along with aminopeptidases and recognizers to commence the sequencing process.</p> </p> </div> </div> <div class="column"> <div data-equalizer-watch> <div class="item-image"> <img decoding="async" src="https://www.quantum-si.com/wp-content/uploads/img_workflow_sequence.svg" alt="" loading="lazy"> </div> <h3>Sequence</h3> <p> <p>Platinum sequences individual peptides by capturing the fluorescent signal from each N-terminal amino acid (NAA) binding event. Aminopeptidases cleave each N-terminal amino acid, exposing the next N-terminal amino acid for recognition, and the process repeats until the whole peptide is sequenced.</p> </p> </div> </div> <div class="column"> <div data-equalizer-watch> <div class="item-image"> <img decoding="async" src="https://www.quantum-si.com/wp-content/uploads/img_workflow_analyze.svg" alt="" loading="lazy"> </div> <h3>Analyze</h3> <p> <p>Data generated during each run is automatically uploaded to the Platinum Analysis Software. Our analysis software delivers single molecule level information about your proteins making protein identification easy to interpret without the need for bioinformatics expertise.</p> </p> </div> </div> </div> </section> </div> </div> <section class="rich-text" data-block_ca3d46a2d7f017902aa156e23f2c6e01 id="rich-text-kinetic-si"> <div class="row"> <div class="column"> <div class="text-center"> <h2>Kinetic Signatures</h2> <h3>Accelerating the Path to Discovery</h3> </div> <div class="row"> <div class="column">A kinetic signature comprises the measurable characteristics of the series of dynamic recognizer events that uniquely identifies a peptide. Kinetic signatures change dynamically with alterations in amino acid and post-translational modifications (PTMs), providing a reliable means to accurately align peptides to their respective proteins. Unlike other technologies, kinetic signatures offer a robust and confident approach to this alignment.</p> </div> <div class="column">As Next-Generation Protein Sequencing becomes more widespread, kinetic signatures will be crucial to identifying every amino acid and every PTM, making Quantum-Si’s technology key to advancing our understanding of the proteome.</p> <h4 class="h3">See Platinum in Action!</h4> <p><a class="button" href="https://www.quantum-si.com/contact/">Request A Demo</a></p> </div> </div> </div> </div> </section> <section class="video-player" data-block_ff77913c3a448c7565fb60aac2d0220f> <div class="row align-center"> <div class="column small-12 xlarge-11 text-center"> <h2 class="underline">Expand the Frontiers of Proteomics With Platinum</h2> <div class="video-wrapper"> <div class="responsive-embed widescreen"> <video src="https://www.quantum-si.com/wp-content/uploads/23_QuantumSi_Interviews_Edgar_Manriquez_v.8_Rebranded-121823.mp4" poster="https://www.quantum-si.com/wp-content/uploads/Edgar_Manriquez_v.8-1024x576.jpg"></video> <div class="play-button"> <span class="material-symbols-outlined">play_arrow</span> </div> </div> <div class="video-controls row large-"> <div class="column small-12"> <div class="slider time-slider" data-slider data-initial-start="0" data-end="100" step="0"> <span class="slider-handle time-slider-handle" data-slider-handle role="slider" tabindex="1"></span> <span class="slider-fill time-slider-fill" data-slider-fill></span> <input type="hidden" class="time-slider-value"> </div> </div> <div class="column"> <div class="row"> <div class="column shrink icons"> <span class="playpause" data-state="play"> <span class="play-icon material-symbols-outlined">play_arrow</span> <span class="pause-icon material-symbols-outlined">pause</span> </span> <span class="stop"></span> <span class="mute" data-state="mute"> <span class="unmute-icon material-symbols-outlined">volume_up</span> <span class="mute-icon material-symbols-outlined">volume_off</span> </span> </div> <div class="column shrink icons show-for-large"> <div class="slider volume-slider" data-slider data-initial-start="50" data-end="100"> <span class="slider-handle volume-slider-handle" data-slider-handle role="slider" tabindex="1"></span> <span class="slider-fill volume-slider-fill" data-slider-fill></span> <input type="hidden" class="volume-slider-value"> </div> </div> <div class="column time"> <span class="time-current">0:00</span> / <span class="time-total">0:00</span> </div> </div> </div> <div class="column text-right shrink"> <span class="pip material-symbols-outlined">picture_in_picture_alt</span> <span class="fs material-symbols-outlined">fullscreen</span> </div> </div> </div> <p> </p> </div> </div> </section> <section class="rich-text" data-block_37bb856590a5d96a0241f9bc0352a6b1 id="rich-text-platinum-d"> <div class="row"> <div class="column"> <h2>Platinum Differs from Mass Spectrometry</h2> <p>Platinum is designed to fit the space, budget, and existing expertise of most labs interested in generating insights from protein sequencing data. Our technology identifies proteins using unique Kinetic Signatures based on the binding kinetics of amino acid recognition events. In contrast, mass spectrometry infers protein identification based on the mass:charge ratio which may not distinguish protein variants of similar size.</p> <div class="alignhalf-div"> <div class="alignright"> <table id="tablepress-1" class="tablepress tablepress-id-1"> <thead> <tr class="row-1"> <th class="column-1"></th><th class="column-2">Mass Spectrometry</th><th class="column-3">Platinum</th> </tr> </thead> <tbody> <tr class="row-2"> <td class="column-1">Expertise</td><td class="column-2">Advanced</td><td class="column-3">Basic</td> </tr> <tr class="row-3"> <td class="column-1">Analysis</td><td class="column-2">Complex</td><td class="column-3">Automated</td> </tr> <tr class="row-4"> <td class="column-1">Lab Space</td><td class="column-2">Designated Space</td><td class="column-3">Benchtop Instrument</td> </tr> <tr class="row-5"> <td class="column-1">Startup Cost</td><td class="column-2">$$$$$</td><td class="column-3">$</td> </tr> <tr class="row-6"> <td class="column-1">Maintenance Cost</td><td class="column-2">$$$</td><td class="column-3">$</td> </tr> </tbody> </table> </div> <p>The Platinum Next-Generation Protein Sequencing workflow is easy to implement and delivers answers quickly – right in your lab without the need for bioinformatics expertise.</p> </div> <p><a class="button" href="https://quantumsi.supremeclients.com/resources/app-notes/comparison-of-protein-sequencing-analysis-of-cdnf-on-platinum-and-mass-spectrometry/">Compare Platinum to Mass Spec</a></p> </div> </div> </section> <section class="rich-text" data-block_2a488c856b40b517d42af0ae0a60deed id="rich-text-platinum-d"> <div class="row"> <div class="column"> <h2>Platinum Differs from Immunoassays</h2> <p>Next-Generation Protein Sequencing provides deeper insights with single-molecule resolution that, unlike immunoassays, enables assessment of what is actually happening at the amino acid level.</p> <div class="row"> <div class="column"><img fetchpriority="high" decoding="async" class="alignnone wp-image-1696 size-full" src="https://www.quantum-si.com/wp-content/uploads/img_immunoassay.svg" alt="" width="653" height="329" /></div> <div class="column"></p> <ul> <li>Verify the specificity of your antibodies by sequencing the target protein.</li> <li>Sequence the protein band from your gel.</li> <li>Uncover what is there, not just what could be there.</li> <li>Interrogate protein variants not resolved by antibodies.</li> </ul> <p><a class="button" href="https://www.quantum-si.com/resources/tech-notes/protein-identification-using-next-generation-protein-sequencing-of-in-gel-digested-proteins/">Sequence Proteins from Gels</a></p> </div> </div> </div> </div> </section> <section class="rich-text" data-block_ed27b2e7288c786978b59bafdc9abf95 id="rich-text-platinum-d"> <div class="row"> <div class="column"> <div class="row"> <div class="column"> <h2>Platinum Differs from Edman Degradation</h2> <p><img decoding="async" class="alignright wp-image-1697 size-full" src="https://www.quantum-si.com/wp-content/uploads/EdmanDegradation.png" alt="" width="540" height="372" srcset="https://www.quantum-si.com/wp-content/uploads/EdmanDegradation.png 540w, https://www.quantum-si.com/wp-content/uploads/EdmanDegradation-300x207.png 300w" sizes="(max-width: 540px) 100vw, 540px" />Platinum Next-Generation Protein Sequencing leverages a one-pot reaction without complex and expensive cyclical chemistry and fluidics to achieve single amino acid resolution. With Platinum technology, you benefit from:</p> <ul> <li>Deeper protein sequencing discoveries.</li> <li>Easy protein sequencing workflows.</li> <li>Affordable protein sequencing solutions.</li> </ul> </div> </div> </div> </div> </section> </div> </section> <div class="footer-bar"><p>RESEARCH USE ONLY. 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