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li{display:table-cell;float:none!important;width:10%;padding:0 15px;border-right:1px solid rgba(0,0,0,0.05)}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:980px!important;margin:0}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{margin:20px 0;padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-transparent #Top_bar .menu > li > a{margin:5px 0}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-top.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-bottom.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span:not(.description){border-right-width:0}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank{padding-left:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:none;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{display:none}#Header_creative #Top_bar .top_bar_right_wrapper{top:0}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0!important}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto;bottom:0}#Header_creative #Top_bar a#header_cart{display:inline-block;float:none;top:3px}#Header_creative #Top_bar a#search_button{display:inline-block;float:none;top:3px}#Header_creative #Top_bar .wpml-languages{display:inline-block;float:none;top:0}#Header_creative #Top_bar .wpml-languages.enabled:hover a.active{padding-bottom:9px}#Header_creative #Top_bar a.button.action_button{display:inline-block;float:none;top:16px;margin:0}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl.nice-scroll #Header_creative{margin-right:10px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul li.hover a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1130px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;filter:alpha(opacity = 97);-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{max-width:1130px;left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}.layout-boxed.header-boxed.nice-scroll #Top_bar.is-sticky{margin-left:-5px}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none}#Top_bar.is-sticky .top_bar_right{top:-4px;height:auto}#Top_bar.is-sticky .top_bar_right_wrapper{top:15px}.header-plain #Top_bar.is-sticky .top_bar_right_wrapper{top:0}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right a{color:rgba(255,255,255,.5)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.3);border-color:rgba(0,0,0,0.1)}}@media only screen and (min-width:768px) and (max-width:1130px){.header_placeholder{height:0!important}}@media only screen and (max-width:1129px){#Top_bar #menu{display:none;height:auto;width:100%;bottom:auto;top:100%;right:1px;position:absolute;margin:0}#Top_bar a.responsive-menu-toggle{display:block}#Top_bar #menu > ul{width:100%;float:left}#Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}#Top_bar #menu ul li a{padding:0 25px;margin:0;display:block;height:auto;line-height:normal;border:none}#Top_bar #menu ul li a:after{display:none}#Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}#Top_bar #menu ul li a span.description{margin:0 0 0 5px}#Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:15px;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Top_bar #menu ul li.hover > 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id="menu-item-26913" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26913 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/e220-focused-ultrasonicator-500239"><span class="ubermenu-target-title ubermenu-target-text">E220</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, 1 to 96 sample batch preparation system</span></a></li></ul></li><li id="menu-item-26914" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26914 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/high-throughput-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">High-throughput Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26914 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26915" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26915 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220-plus-focused-ultrasonicator-500569"><span class="ubermenu-target-title ubermenu-target-text">LE220-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High-throughput, 1 to 96 tube capacity</span></a></li><li id="menu-item-26916" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26916 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220r-plus-focused-ultrasonicator-500578"><span class="ubermenu-target-title ubermenu-target-text">LE220R-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Robotic version of the LE220-plus</span></a></li><li id="menu-item-30065" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30065 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/le220rsc-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">LE220Rsc</span></a></li></ul></li><li id="menu-item-26917" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26917 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/on-deck-automation-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">On-Deck Automation Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26917 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26918" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26918 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/r230-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">R230</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">On-deck, next generation Focused-ultrasonicator</span></a></li></ul></li><li id="menu-item-26919" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26919 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/cryoprep"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP<sup>®</sup> Extraction Systems</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26919 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26920" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26920 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp01-cryoprep-manual-dry-pulverizer-500230"><span class="ubermenu-target-title ubermenu-target-text">CP01 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 15 mg to 2 g</span></a></li><li id="menu-item-26921" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26921 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp02-cryoprep-automated-dry-pulverizer-110v-500001"><span class="ubermenu-target-title ubermenu-target-text">CP02 Automated Dry Pulverizer</span></a></li><li id="menu-item-26922" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26922 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp03-cryoprep-manual-dry-pulverizer-500049"><span class="ubermenu-target-title ubermenu-target-text">CP03 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 2 to 5 g</span></a></li></ul></li><li id="menu-item-26923" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26923 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/sonolab-software"><span class="ubermenu-target-title ubermenu-target-text">SonoLab Software</span></a></li></ul></li><li id="menu-item-26927" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26927 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents"><span class="ubermenu-target-title ubermenu-target-text">Kits & Reagents</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26927 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-41083" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-41083 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truprep-protein/"><span class="ubermenu-target-title ubermenu-target-text">truPREP® Protein Kits</span></a></li><li id="menu-item-35108" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-35108 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truxtrac-ffpe-na-auto-96-kits"><span class="ubermenu-target-title ubermenu-target-text">truXTRAC® FFPE SMART Solutions</span></a></li><li id="menu-item-26931" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26931 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/cryoprep-0"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Starter Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cryoPREP Sample Tubes & Holders</span></a></li><li id="menu-item-26933" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26933 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/nucleic-acids-shearing-kits-and-buffers"><span class="ubermenu-target-title ubermenu-target-text">Nucleic Acids Shearing Kits and Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">truSHEAR, truPOP, DNA Verification Kits</span></a></li><li id="menu-item-26929" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26929 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-ffpe-kits"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> FFPE Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">FFPE Biomolecule Extraction Kits</span></a></li><li id="menu-item-26934" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26934 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-other"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cfDNA, DBS, MALDI-TOF</span></a></li><li id="menu-item-26935" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26935 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/protein-extraction-buffers"><span class="ubermenu-target-title ubermenu-target-text">Protein Extraction Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Native, Total Protein, & Detergent-free Buffers</span></a></li><li id="menu-item-26930" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26930 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truchip"><span class="ubermenu-target-title ubermenu-target-text">truChIP<sup>®</sup></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Chromatin Shearing Kits</span></a></li></ul></li><li id="menu-item-26936" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26936 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables"><span class="ubermenu-target-title ubermenu-target-text">Consumables – Tubes and Vials</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26936 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26938" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26938 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/afa-tube"><span class="ubermenu-target-title ubermenu-target-text">AFA-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Laser etched polymer vessel for high-throughput workflows</span></a></li><li id="menu-item-26939" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26939 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/microtube"><span class="ubermenu-target-title ubermenu-target-text">microTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Small sample volumes, 130 µl or less</span></a></li><li id="menu-item-26940" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26940 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/millitube"><span class="ubermenu-target-title ubermenu-target-text">milliTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">1 ml or 2 ml sample volume. Good for chromatin shearing</span></a></li><li id="menu-item-26942" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26942 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/other-afa-tubes"><span class="ubermenu-target-title ubermenu-target-text">Other AFA-TUBEs</span></a></li><li id="menu-item-26943" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26943 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/afa-process-vessels/"><span class="ubermenu-target-title ubermenu-target-text">AFA Process Vessels</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, low temperature processing</span></a></li><li id="menu-item-26944" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26944 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/g-tube"><span class="ubermenu-target-title ubermenu-target-text">g-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">DNA shearing fragments 6 to 20 kb</span></a></li></ul></li><li id="menu-item-26945" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26945 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Consumables</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26945 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26947" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26947 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables/tissuetube"><span class="ubermenu-target-title ubermenu-target-text">tissueTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Tissue processing devices</span></a></li></ul></li><li id="menu-item-26949" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26949 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories"><span class="ubermenu-target-title ubermenu-target-text">Accessories</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26949 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26952" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26952 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/automation-accessories"><span class="ubermenu-target-title ubermenu-target-text">Automation Accessories</span></a></li><li id="menu-item-41270" class="ubermenu-item ubermenu-item-type-post_type ubermenu-item-object-page ubermenu-item-41270 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/r230-accessories"><span class="ubermenu-target-title ubermenu-target-text">R230 Accessories</span></a></li><li id="menu-item-26953" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26953 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/m220-accessories"><span class="ubermenu-target-title ubermenu-target-text">M220 Accessories</span></a></li><li id="menu-item-26954" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26954 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/me220-accessories"><span class="ubermenu-target-title ubermenu-target-text">ME220 Accessories</span></a></li><li id="menu-item-30718" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30718 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/ml230-accessories"><span class="ubermenu-target-title ubermenu-target-text">ML230 Accessories</span></a></li><li id="menu-item-26955" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26955 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/s220-accessories"><span class="ubermenu-target-title ubermenu-target-text">S220 Accessories</span></a></li><li id="menu-item-26956" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26956 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-evolution-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i> Accessories</span></a></li><li id="menu-item-26957" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26957 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220 Accessories</span></a></li><li id="menu-item-26958" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26958 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/l-series-accessories"><span class="ubermenu-target-title ubermenu-target-text">L-Series Accessories</span></a></li><li id="menu-item-26959" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26959 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp01-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP01 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style="color: #4b4b4b;text-align: left" class="vc_custom_heading vc_do_custom_heading" >The use and utility of Covaris Adaptive Focused Acoustics®( AFA®) technology is continuously growing and expanding. Covaris has been referenced in over 75,000 publications, and that number continues to grow. We are proud to share the collection of Covaris Scientific posters to educate and inspire our customers on their continued quests for discovery.</h5> <div class="wpb_text_column wpb_content_element vc_custom_1589280178859" > <div class="wpb_wrapper"> <p><img class="alignnone size-full wp-image-41362" src="https://www.covaris.com/wp/wp-content/uploads/Screenshot-2024-04-19-113309-1.png" alt="Journal Citations" width="496" height="306" /></p> </div> </div> <div class="vc_row wpb_row vc_inner vc_row-fluid resources_active vc_custom_1588756649693 vc_row-has-fill vc_row-o-equal-height vc_row-flex"><div class="protocol_heading wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner vc_custom_1588749372342"><div class="wpb_wrapper"><h6 style="color: #293e53;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h2 vc_custom_1588749903360" >Posters</h6></div></div></div><div class="protocol_filter wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner"><div class="wpb_wrapper"><p>Sort By</p><select class="covaris_resources_filter"><option selected="selected" value="popularity">Popularity</option><option value="select">Name</option></select> <div class="wpb_text_column wpb_content_element vc_custom_1588756839218" > <div class="wpb_wrapper"> </div> </div> </div></div></div></div> <div class="wpb_text_column wpb_content_element vc_custom_1588756457325" > <div class="wpb_wrapper"> <div class="protocol_item"> <div class="protocol_filter_count"></div> <p>Items</p> </div> </div> </div> <div class="covaris_resource_content"> <li> <h4>An Automated High-throughput ChIP-Seq Workflow using Covaris Adaptive Focused Acoustics® (AFA®) System for Accelerated IP</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at AGBT 2020 with University of California San Diego: </strong>ChIP-Sequencing (ChIP-Seq) combines chromatin immunoprecipitation (ChIP) with DNA sequencing and has been highly used for studying DNA-protein interactions. Yet, most implementations of ChIP-seq are inefficient and slow requiring 2 to 3 days for completion of the complicated and often manual workflow. To overcome these issues, we combined our validated automated ChIP-seq (Busby et al., Epigenetics & Chromatin, 2016) with the Covaris Adaptive Focused Acoustics (AFA) technology to streamline and significantly reduce the overall workflow time for ChIP-seq. In our study, we tested the ability of AFA to enhance the binding kinetics of antibody-epitope association, improve signal-to-noise ratios, and decrease total processing time. We evaluated a range of epitopes including major histone modifications such as H3K4me3 which is associated with open chromatin and forms narrow peaks, H3K27me3 which is linked to repressed chromatin and binds to wide regions, and H3K27ac associated with open chromatin, and both wide enhancer loci as well as narrow promoter regions. Additionally, we are also studied the capacity of the AFA to augment the IP step of DNA associated proteins such as CTCF and validated our test conditions using two cell types.</h5> <div class="gated-content" data-resource="https://www.covaris.com/wp/wp-content/uploads/2020/07/2020-AGBT-Poster_UCSD.pdf"> <a class="js-gated-btn" href="#" target="_blank" onclick="post_count_vistors(this)" data-id="28317" rel="nofollow">Download PDF</a> </div> </li> <li> <h4>High Molecular Weight DNA Sample and Library Preparation For Long-Read Sequencing Using Clinical Tissue Specimens</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at AGBT 2020 with Circulomics</strong></h5> <a href="/wp/wp-content/uploads/2020/07/2020-AGBT-Poster-Circulomics.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="28324" rel="nofollow" >Download PDF</a> </li> <li> <h4>A Robust and Scalable Workflow for Extraction of DNA, RNA, and Protein from FFPE Tissues</h4> <h6>Quick Guide</h6><h5> Application: FFPE Extraction </h5> <h5 style="margin-top:-10px;"><strong>Presented at EACR 2023</strong></h5> <a href="https://www.covaris.com/wp/wp-content/uploads/EACR-Poster-2023.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="39994" rel="nofollow" >Download PDF</a> </li> <li> <h4>Comprehensive Sample Preparation for Proteins Enabled by AFA® Technology</h4> <h6>Quick Guide</h6><h5> Application: AFA Technology, Proteomics </h5> <h5 style="margin-top:-10px;"><strong>US HUPO 2023 Poster: </strong> Sample preparation can significantly impact data quality derived from complex samples. This is especially important for proteomics research, owing to diversity observed in proteins, which cannot be amplified. Covaris’ Adaptive Focused Acoustics® (AFA®) Technology can address a wide variety of sample types (FFPE, LCM, fresh tissue, cells, bacteria, yeast), while increasing speed, throughput, reproducibility, and reliability. AFA is compatible with other purification approaches like SP3 or S-Trap and extensively used for bottom-up proteomics to targeted quantitation of proteins in complex biological matrices.</h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/US-HUPO-2023-Poster.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="38631" rel="nofollow" >Download PDF</a> </li> <li> <h4>Adaptive focused acoustic lysis for high-throughput shotgun metagenomics and proximity ligation</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at AGBT 2020 with Phase Genomics</strong>: Rapid and thorough lysis of diverse metagenomic samples remains an important challenge in the study of microbial communities using all modern genomic methods. This is also true for Phase Genomics’ ProxiMeta™ platform, which enables accurate metagenomic deconvolution and mobile element host association using Hi-C proximity ligation sequencing. This technology unlocks the ability to track the hosts of antimicrobial resistance and virulence genes borne on mobile elements in settings ranging from healthcare, food production, to agriculture and waste management. However, to be a useful surveillance tool, ProxiMeta must be amenable to high- throughput methods. Representation of diverse organisms found within metagenomic samples can be severely skewed by differential lysis owing to variation in outer membrane and cell wall composition. Differential lysis is typically minimized through a combination of chemical and physical lysis using detergents and physical disruption (bead beating). Bead beating is effective but limits the throughput of metagenomics analysis because it is not compatible with multi-well plates and liquid handling. We overcome this limitation using the Covaris Adaptive Focused Acoustics™ (AFA) technology showing that it is able to rapidly lyse complex microbial communities composed of diverse organisms including Gram-negative bacteria, Gram-positive bacteria, and fungi. We observe a beta-diversity Jaccard index of >0.9 when comparing results of traditional bead beat lysis and AFA. Furthermore, AFA lysis speeds up the ProxiMeta protocol, saving hours when scaled to 96-well plates, delivering accurate metagenomes and mobile element host identification faster than ever.</h5> <a href="/wp/wp-content/uploads/2020/07/2020-AGBT-Poster_Phase-Genomics.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="28322" rel="nofollow" >Download PDF</a> </li> <li> <h4>Accurate and Cost-Effective Discovery of Active Gene Regulatory Interactions using Arima-HiChiP</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at AGBT 2020 with Arima Genomics</strong>: Precise spatiotemporal control of gene regulation is fundamental to normal biological processes and the development of disease. Therefore, detection of gene regulatory interactions is a critical component of understanding the biology of gene regulatory elements. Proximity ligation assays coupled with NGS provide a means to measure chromatin interactions within the nucleus. For example, Hi-C measures the frequency of genomic interactions at a genome-wide scale, and has become a mainstream technology for detecting genome structures ranging from genomic compartments, TADs, to chromatin loops, collectively uncovering a structural framework for gene regulation. Despite this utility, HiC is a genome-wide sequencing assay with relatively increased costs for high resolution analyses (>600M reads). Furthermore, HiC doesn’t directly enrich for gene regulatory interactions, such as those between promoters and enhancers. To address this and build upon our existing Arima-HiC kit platform, we have developed Arima-HiChIP. Arima-HiChIP integrates our Arima-HiC technology with ChIP-seq, thereby enriching for gene regulatory interactions associated with active regulatory elements. The optimized Arima-HiChIP workflow begins with ~3 million cells, and can be completed in 3 days. Our Arima-HiChIP workflow is currently designed for integration with Covaris TruChIP chromatin shearing kit and focused-ultrasonicators for chromatin shearing. We have optimized the Arima-HiChIP workflow for high resolution analysis of gene regulatory interactions associated with histone modifications, such as H3K27ac or H3K4me3, with reduced sequencing requirements (<200M reads). We have also built-in QC assays for the HiC portion of the workflow, chromatin shearing efficiency, ChIP enrichment, and library complexity. On the bioinformatics side, we have validated two open-source HiChIP analysis pipelines, MAPS and FitHiChIP. From extensive analyses of public and internal HiChIP datasets, we propose a new standardized framework and metrics for analytical QC of HiChIP data. We aim to expand our Arima-HiChIP technology towards optimized protocols for analysis of transcription factors and low input samples.</h5> <a href="/wp/wp-content/uploads/2020/07/2020-AGBT-Poster_Arima-Genomics.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="28452" rel="nofollow" >Download PDF</a> </li> <li> <h4>Fully Automated Extraction of High-Quality Total Nucleic Acids from FFPE Specimens using Covaris truXTRAC® FFPE SMART Solutions & Hamilton Robotics</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at SLAS 2024: </strong>Covaris, Hamilton, and Labcorp (OmniSeq®) solution merges Hamilton’s Microlab® STAR™ with Covaris’ truXTRAC® FFPE SMART Solutions technology for FFPE processing • Highlights the potential of the Assay-Ready Workstation (ARW) in improving FFPE DNA/RNA extraction processes, including those in the OmniSeq INSIGHT assay workflow • DNA and RNA from FFPE Specimens purified automatically by ARW • DNA and RNA quality metrics and yield analyzed at Labcorp • TruSight® Oncology 500 (TSO 500, Illumina®) next-generation sequencing (NGS) metrics analyzed by Labcorp • Samples extracted using the ARW and truXTRAC FFPE SMART Solutions outperform the current clinically-validated extraction workflow in terms of yield, purity, and downstream DNA and RNA NGS performance</h5> <div class="gated-content" data-resource="https://www.covaris.comhttps://www.covaris.com/wp/wp-content/uploads/SLAS-2024-Poster.pdf"> <a class="js-gated-btn" href="#" target="_blank" onclick="post_count_vistors(this)" data-id="41125" rel="nofollow">Download PDF</a> </div> </li> <li> <h4>Evaluation of a New Protein Extraction Method Using an Advanced Acoustic Technology for Identification of Filamentous Fungi by MALDI-TOF MS</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented with The Johns Hopkins Hospital: </strong>While matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has become a mainstay for clinical labs to identify most bacteria and yeast, its application for filamentous fungi (mold) identification (ID) is still limited. In this study, we assessed a new protein extraction method that generated spectra suitable for MALDI-TOF MS using Adaptive Focused Acoustics<sup class="reg">® </sup>with a Focused-ultrasonicator. (Covaris<sup class="reg">®</sup> M220, Woburn, MA, USA).</h5> <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/Rachel-Mold-Covaris.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="19212" rel="nofollow" >Download PDF</a> </li> <li> <h4>Comprehensive Sample Prep Workflow for Deparaffinization, Extraction, Purification, & Accelerated Digestion of Proteins from Formalin-Fixed Paraffin-Embedded Tissue</h4> <h6>Quick Guide</h6><h5> Application: Extraction from FFPE Samples, FFPE Extraction </h5> <h5 style="margin-top:-10px;"><strong>Presented at ASMS 2024</strong>: Formalin-fixed paraffin-embedded (FFPE) tissue samples are a valuable resource for studying the molecular mechanisms of disease. FFPE tissue samples are widely used in genomics and have been gaining popularity in proteomics. Despite several technical advancements in LC-MS, identification or quantitation of proteins in tissues embedded in paraffin can be complicated owing to protein cross-linking. Most sample preparation workflows for FFPE samples often result in highly degraded proteins with low yields. Adaptive Focused Acoustics® (AFA®) Technology is extensively used for efficient extraction, purification, and expedited digestion of proteins from complex biological matrices, such as FFPE. In this study, we report development of comprehensive and robust workflows with Covaris technology for deparaffinization, extraction, purification, and digestion of proteins starting with just one 10 µm FFPE scroll.</h5> <a href="https://www.covaris.com/wp/wp-content/uploads/ASMS-2024-FFPE-Poster.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="41566" rel="nofollow" >Download PDF</a> </li> <li> <h4>Comprehensive Workflow for Extraction, Purification, and Accelerated Digestion of Proteins from Mammalian Cells – ASMS 2024 Poster</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at ASMS 2024</strong>: Over the last twenty years, breakthroughs in analytical technologies like LC-MS have transformed proteomics from a purely research-focused area to a field with significant applications in clinical research and biopharma, directly benefiting patients. However, achieving high quality proteomics data is not possible without a robust sample preparation protocol. Adaptive Focused Acoustics® (AFA®) Technology has been extensively used in genomics and recently found widespread use in efficient extraction, purification, and expedited digestion of proteins from complex matrices. In this study, we report development of a comprehensive workflow with Covaris truPREP® Protein Kit that ensures extraction, purification, and accelerated digestion for a wide range of mammalian cells</h5> <a href="https://www.covaris.com/wp/wp-content/uploads/ASMS-2024-Cell-Poster.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="41569" rel="nofollow" >Download PDF</a> </li> <li> <h4>Robust Sample Preparation Workflows for Every Analyte from FFPE Samples</h4> <h6>Quick Guide</h6><h5> Application: FFPE Extraction </h5> <h5 style="margin-top:-10px;"><strong>Presented at SEASR 2023</strong>: Formalin-fixation and paraffin-embedding (FFPE) of tissue samples are routinely used in clinical histopathology. While FFPE preserves tissue architecture, removal of paraffin is an essential prerequisite to achieve high quality LC-MS data. Xylol, despite all its efficacy presents severe health risks and repeated steps of xylol removal compromises assay reproducibility. Superior sample preparation not only ensures removal of unwanted matrix components, but also ensures reliability, reproducibility and robustness of the assays. Adaptive Focused Acoustics® (AFA®) Technology (Figure 1) works via a focused short-wavelength energy that can be efficiently used not only for lysis of samples, but also for active deparaffinization, and extraction, isolation, purification of critical analytes (DNA, RNA, Proteins) from any tissue samples. In this study, we highlight two different workflows that ensure - i) generation of high quality and yield of tNA (Total nucleic acids - DNA and RNA), and ii) extraction, purification, and digestion of global or targeted proteins - from any tissue types in FFPE.</h5> <a href="https://www.covaris.com/wp/wp-content/uploads/SEASR-2023-Poster.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="40088" rel="nofollow" >Download PDF</a> </li> <li> <h4>Focused Acoustic Enabled High Quality Extraction of both DNA and RNA and Subsequent Bead Purification from an FFPE Sample</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at AMP 2020: </strong>Formalin Fixed Paraffin Embedded (FFPE) tissue samples have become a primary sample type for clinical oncology Next-Generation Sequencing (NGS) based assays [1]. As the demand for sequencing in diagnostics has increased dramatically, molecular biology laboratories receive FFPE sections without having significant control over how the samples are shipped and stored [2]. This can impact sample processing efficiency, sample loss probability during transfer, and performance of automated and semiautomated purification workflows. To enable a streamline sample preparation protocol, we have developed a new system for extraction. Here we present a novel semi-automated FFPE co-extraction yielding high quality RNA and DNA from the same sample using the ML230 Focused-ultrasonicator, truXTRAC® FFPE tNA Ultra Kit-Magnetic Bead Purification, and the KingFisher Duo Prime. Adaptive Focused Acoustics® (AFA®), emitted by Covaris Focused-ultrasonicators, simultaneously emulsifies paraffin into an aqueous buffer and rehydrates the tissue; thereby, enabling efficient Proteinase K digestion and de-crosslinking kinetics. We demonstrate robust FFPE extraction yielding high quality nucleic acids using this novel method with the ML230</h5> <a href="/wp/wp-content/uploads/resources_pdf/AMP-2020-Poster.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="31268" rel="nofollow" >Download PDF</a> </li> <li> <h4>Standardized Sample preparation Workflows for Clinical Proteomics</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at ASMS 2020</strong></h5> <a href="https://www.covaris.com/wp/wp-content/uploads/2020/06/ASMS_2020_Poster.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="27681" rel="nofollow" >Download PDF</a> </li> <li> <h4>Scalable FFPE Sample Preparation Method for Clinical Proteomics LC-MS Assays</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at ASMS 2022 with Memorial Sloan Kettering Cancer</strong> Center: We present a high-throughput workflow for the preparation of FFPE tissue samples for downstream mass spectrometry-based proteomics analysis. Tissue homogenization through digestion are performed in single well on a 96-well plate in approximately 8 hours allowing for same day analysis.</h5> <a href="/wp/wp-content/uploads/resources_pdf/Scalable-FFPE-Sample-Preparation-Method-for-Clinical-Proteomics-LC-MS-Assays-ASMS2022.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="36322" rel="nofollow" >Download PDF</a> </li> <li> <h4>From Tissues to LC-MS-ready Protein Digests: Automated Sample Preparation Enabled by Adaptive Focused Acoustics® (AFA®) Technology</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at ASMS 2022</strong>: LC-MS based analysis of peptide hydrolysates obtained from digested proteins is important for pre-clinical evaluation of compound libraries, as well as for discovery and analysis of biochemical pathways in translational medicine. While the last couple of decades witnessed tremendous growth in analytical technology such as high-resolution accurate mass spectrometry, liquid chromatography, and informatics (enabling workflows from data analysis to data management), lagging progress in sample extraction from biological matrices and purification of analytes often results in inefficient and poor-quality data, regardless of the end detection analytical tools. Here, we introduce a comprehensive and fully automatable sample preparation workflow that incorporates Adaptive Focused Acoustics (AFA)-enabled efficient cell lysis, protein extraction, protein/bead binding, and on-bead trypsin digestion. This workflow requires only three liquid transfer steps, resulting in a robust process and guaranteeing high quality data.</h5> <a href="/wp/wp-content/uploads/resources_pdf/From-Tissues-to-LC-MS-ready-Protein-Digests-ASMS2022.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="36320" rel="nofollow" >Download PDF</a> </li> <li> <h4>Epigenomics at the Speed of Sound: High-throughput Epigenomic Mapping of Low Cell Input Samples using Acoustic Enhanced Immunoprecipitation</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at AACR 2022</strong>: Mapping the epigenetic landscape of Myelodysplastic Syndrome (MDS) and Acute Myeloid Leukemia (AML) is essential for understanding gene regulatory mechanisms during cell fate changes, disease onset, progression, as well as for optimizing therapeutic regime. In addition, these information hold immense potential for accurate identification of disease biomarkers, cell of origin, and targets for epigenetic therapy. However, existing chromatin immunoprecipitation sequencing (ChIP-Seq) workflows require significant cellular material and are very time-consuming, requiring cumbersome optimization. Moreover, cellular heterogeneity can influence the accuracy of results from the perspective of sample preparation efficiency, and population-biased effects. Both normal and malignant bone marrow are heterogeneous in cell type and stage of differentiation that leads to imprecise results regarding epigenetic determinates. In our study, we implemented Covaris’ Adaptive Focused Acoustics® (AFA®) technology to miniaturize sample volumes for both chromatin shearing and immunoprecipitation in a 96-well format.</h5> <a href="https://covaris.com/wp/wp-content/uploads/resources_pdf/Epigenomics-at-the-Speed-of-Sounds-AACR-2022.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="36530" rel="nofollow" >Download PDF</a> </li> <li> <h4>Efficient Sample Preparation with Covaris AFA® Technology Enhances Confidence in Illumina® TruSight™ Oncology 500 Assay</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at AACR 2022</strong>: A robust, reliable, and extensive NGS library can enable the process of detection, diagnosis and survival chances of cancer. DNA fragmentation enabled by AFA Technology ensures reliable and reproducible fragmentation of DNA. The ML230 focused-ultrasonicator can be used efficiently for comprehensive pan-cancer panels such as Illumina®'s TruSight™ Oncology 500 assay.</h5> <a href="http://covaris.com/wp/wp-content/uploads/resources_pdf/AFA-and-TruSight-AACR-2022.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="36528" rel="nofollow" >Download PDF</a> </li> <li> <h4>A Robust LC-MS analysis of C. albicans – Differential Protein Expression using AFA focused ultrasonication, SP3 and TMT labelling</h4> <h6>Quick Guide</h6><h5> </h5> <h5 style="margin-top:-10px;"><strong>Presented at ASMS 2022</strong>: <em>Candida albicans</em> is the most common human pathogenic yeast and a member of the human gut flora [1]. It can reside harmlessly on the skin, in the oral cavity, and/or in the urogenital and gastrointestinal tracts causing cutaneous and mucocutaneous candidiasis [2]. It is a polymorphic organism, being able to grow in yeast form, or in hyphal and pseudohyphal types, which form filamentous structures [1]. C. albicans can cause life-threatening systemic infections: the hyphal form (which can be induced in vitro using BSA [3]) has an important role in causing disease by invading epithelial cells and causing tissue damage [4]. This study aimed at analyzing the protein determinants differentially expressed between yeast and hyphal forms</h5> <a href="/wp/wp-content/uploads/resources_pdf/AFA-with-SP3-and-TMT-ASMS-2022-Poster.pdf" target="_blank" onclick="post_count_vistors(this)" data-id="36328" rel="nofollow" >Download PDF</a> </li> <div class="modal gated-content-modal"> <div class="gated-container"> <div class="gated-content"> <div class="gated-inner"> <span class="gated-content-modal-close">×</span> <script type="text/javascript"></script> <div class='gf_browser_ie gf_browser_ie7 gform_wrapper gravity-theme gform-theme--no-framework gated-content-form_wrapper' data-form-theme='gravity-theme' data-form-index='0' id='gform_wrapper_35' ><div id='gf_35' class='gform_anchor' tabindex='-1'></div> <div class='gform_heading'> <h2 class="gform_title">Download Resource</h2> <p class='gform_description'>Please submit the form below to access this resource.</p> <p class='gform_required_legend'>"<span 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