Transgenic Gene Targeting Core | Gladstone Institutes

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class="title page-title"><span class="field field--name-title field--type-string field--label-hidden">Transgenic Gene Targeting Core</span> </h1> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="clearfix main-content region--dark-typography region--white-background region--no-separator"> <div class="landing-page-container" > <div class="clearfix main-content__container"> <div class="row"> <section> <div class="clearfix main-content__section" > <div class="region region-content"> <div id="block-showcase-lite-content" class="clearfix block block-system block-system-main-block"> <div class="content"> <article data-history-node-id="3274" class="node node--type-core-mini-sites node--promoted node--view-mode-full clearfix"> <div class="node__container"> <div class="node__main-content clearfix"> <header class="node__header"> </header> <div id="mini-core-tabs" class="row"> <div id="subtabs" class="subtabs"> <ul class="sudonav"> <li class="tab"><a href="#overview"><span>Overview</span></a></li> <li class="tab"><a href="#services"><span>Services</span></a></li> <li class="tab"><a href="#fees-and-scheduling"><span>Fees and Scheduling</span></a></li>  </ul> <div class="content-wrap"> <section id="overview"> <div id="intro"> <div class="container"> <p class="large">The Transgenic Gene Targeting Core has been an integral part of Gladstone’s research for more than 20 years, producing mouse models as a service to investigators at Gladstone, UC San Francisco, and other research institutions. Transgenic and knock-out technologies enable scientists to learn how specific genes and proteins, or their variants, function in living animals with defined genetic backgrounds.</p> </div> </div> <div id="manager"> <div class="container"> <h2>Contact</h2> <div class="field field--name-field-core-contact field--type-entity-reference field--label-hidden field--entity-reference-target-type-user clearfix field__item"><article class="user user--coredirector"> <div class="user__compact"> <p> <b>Junli Zhang, MD </b><br> Core Director <br> <a class="cracked" href="mailto:junli.zhang@gladstone.ucsf.edu">Email</a> <a class="forflow" href="mailto:flow-core@gladstone.ucsf.edu">Email</a><br> </p> </div> <style> .forflow {display:none} </style> </article> </div> </div> </div> <div class="corebk-xtra">   </div>  <div class="core-members"> <div class="container"> <h2>Core Members</h2> <div class="views-element-container"><div class="view view-user-admin-people view-id-user_admin_people view-display-id-core_members js-view-dom-id-297ab87c52794ce159fd8c40cedfee9b0a4a9243952676a55024e01757abd862"> <div class="view-content"> <div class="views-view-grid horizontal cols-2 clearfix"> <div class="views-row clearfix row-1"> <div class="views-col col-1" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Carlos Benitez</b><br>Research Associate II<br></p> </div> </article> </div> <div class="views-col col-2" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Sid Espineda</b><br>Animal Care Facility Supervisor<br></p> </div> </article> </div> </div> <div class="views-row clearfix row-2"> <div class="views-col col-1" style="width: 50%;"><article class="user user--compnomail"> <div class="user__compact"> <p><b>Junli Zhang</b><br>Core Director<br></p> </div> </article> </div> </div> </div> </div> </div> </div> </div> </div> <div class="corebk1">   </div>  </section> <section id="services"> <div class="container"> <h2 class="tabtop">Services Provided</h2> <p>Gladstone’s Transgenic Gene Targeting Core creates genetically modified mouse models as services for researchers at Gladstone, UC San Francisco, and outside institutions.</p> <p>Researchers provide the reagents (DNA, ES cells, Cas9, gRNAs, etc.). The core performs microinjection or electroporation and transfers the embryos into recipient female mice.</p> <p><a name="svc-1"></a></p> <p><a href="#svc-1">CRISPR-Mediated Knock-Out and Knock-In Service</a></p> <p><a href="#svc-2">Microinjection of DNA</a></p> <p><a href="#svc-3">Microinjection of Mouse ES Cells</a></p> <h3>CRISPR-Mediated Knock-Out and Knock-In Service</h3> <p>CRISPR-Mediated Knock-Out and Knock-In mice: The core injects or electroporates the CRISPR reagents into C57BL/6 and FVB embryos. Contact the Core if you wish to use another strain.</p> <ul> <li>Basic service: One day of microinjection or electroporation with a minimum guarantee of 75 injected or electroporated embryos transferred.<a name="svc-2"></a></li> <li>Premium service: Two days of microinjection or electroporation with a minimum guarantee of 150 injected or electroporated embryos transferred, or 10 pups born, or 2 transgenic founders produced, whichever comes first.</li> </ul> <h3>Microinjection of DNA</h3> <p>To generate transgenic animals, plasmid or BAC DNA can be injected in C57BL/6 or FVB/n strains. Contact the Core if you wish to use another strain.</p> <ul> <li><strong>Basic service:</strong> One day of microinjection with a minimum guarantee of 75 injected embryos transferred.<a name="svc-3"></a></li> <li><strong>Premium service:</strong> Two days of microinjection with a minimum guarantee of 150 injected embryos transferred, or 10 pups born, or 2 transgenic founders produced, whichever comes first.</li> </ul> <h3>Microinjection of Mouse ES Cells</h3> <p>Researchers provide the ES cell clones to inject. The core injects the clones into the blastocysts of day-3.5 embryos, and transfers the embryos into recipient female mice.</p> <ul> <li><strong>Basic Service:</strong> Microinjection of one clone with a minimum guarantee of 40 injected embryos transferred, or 10 pups born, or two chimeric mice produced, whichever comes first.</li> <li><strong>Premium Service:</strong> Microinjection of 2–4 clones with a minimum guarantee of 90 injected embryos transferred, or 20 pups born, or four chimeric mice produced, whichever comes first.</li> </ul> </div> <div class="corebk2">   </div> </section> <section id="fees-and-scheduling"> <div class="container"> <h2 class="tabtop">Fees and Scheduling</h2> <p><a href="https://cgec.ucsf.edu/cell-and-genome-engineering-core">Visit iLab for fee information</a> and to schedule services.</p> <h2>How to Use the Core</h2> <p>You must have an approved IACUC protocol for each transgenic or knock-out/knock-in mouse model you wish to create.</p> <ol> <li><strong>Contact Us</strong><br /> <a href="mailto:junli.zhang@gladstone.ucsf.edu">Email us about your project</a> before you request services in iLab.<br /> If you have any questions, contact us by email. We do not design constructs, however, we have access to many experts who can help with this step.</li> <li><strong>Request Services</strong><br /> Gladstone’s Transgenic Gene Targeting Core uses iLab to streamline the process of ordering, tracking, and billing. <a href="https://my.ilabsolutions.com/service_center/show_external/2999/transgenic-gene-targeting-core">Register for an iLab account to request services.</a><br /> For users outside Gladstone, we require a PO number in order to initiate a project.</li> <li><strong>Schedule Service</strong><br /> Once we receive the request, we will schedule your experiments. While waiting times change based on demand, your experiment can typically be scheduled 4–5 weeks from the time we receive the request.</li> <li><strong>Submit Samples</strong><br /> You need to submit your DNA and/or CRISPR reagents before we can schedule their injection. For ES cells, we schedule the injections when we receive your request. 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