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class="elementor-background-overlay"></div> <div class="elementor-container elementor-column-gap-no"> <div class="elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-21918755" data-id="21918755" data-element_type="column"> <div class="elementor-widget-wrap elementor-element-populated"> <div class="elementor-element elementor-element-27aec94f elementor-widget__width-initial elementor-widget elementor-widget-heading" data-id="27aec94f" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h1 class="elementor-heading-title elementor-size-default">Pricing</h1> </div> </div> <div class="elementor-element elementor-element-12b51acc elementor-widget__width-initial elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="12b51acc" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-5fd9ac80 elementor-widget__width-initial elementor-widget elementor-widget-heading" data-id="5fd9ac80" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">Standard Analytical Services</h2> </div> </div> </div> </div> </div> </section> <div class="elementor-element elementor-element-ba2a974 e-flex e-con-boxed e-con e-parent" data-id="ba2a974" data-element_type="container" data-settings="{"background_background":"classic","animation":"none"}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-107b7ce e-con-full e-flex e-con e-child" data-id="107b7ce" data-element_type="container"> <div class="elementor-element elementor-element-229a844 e-flex e-con-boxed e-con e-child" data-id="229a844" data-element_type="container" data-settings="{"background_background":"classic","animation":"none","animation_delay":400}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-ff628db e-flex e-con-boxed e-con e-child" data-id="ff628db" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-2a5fc4d elementor-widget elementor-widget-heading" data-id="2a5fc4d" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Pricing – DNA Barcoding</h3> </div> </div> <div class="elementor-element elementor-element-069d9f2 elementor-widget elementor-widget-text-editor" data-id="069d9f2" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>All prices are in Canadian dollars and apply to submissions involving 96-well plates of tissues. This price schedule is based on the use of standard CCDB protocols and applies to all researchers and organizations. Standard protocols may not be appropriate for all tissue types (e.g. older museum specimens or forensic samples) and additional charges may apply.</p> </div> </div> <div class="elementor-element elementor-element-e84347e elementor-widget elementor-widget-text-editor" data-id="e84347e" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p><a style="color: #ffcc00;" href="https://ccdb.ca/wp/pricing-genomics/">Go here</a> for pricing of our other genomic and metagenomic services.</p> </div> </div> <div class="elementor-element elementor-element-e50b882 elementor-widget elementor-widget-text-editor" data-id="e50b882" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>CCDB carries out the following steps:</p><ul class="check-list" style="margin-top: 10px; padding-left: 25px;"><li><strong>CCDB Admin:</strong> Plate reception, specimen data validation, billing.</li><li><strong>CCDB Analytical Lab:</strong> DNA extraction, PCR amplification of the barcode region(s), bidirectional amplicon sequencing (where applicable), trace file assembly, uploading trace files and sequences to BOLD.</li><li><strong>BOLD Systems:</strong> Data validation and storage.</li></ul> </div> </div> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-f030cec e-con-full e-flex e-con e-child" data-id="f030cec" data-element_type="container" data-settings="{"background_background":"classic","animation":"none","animation_delay":600}"> <div class="elementor-element elementor-element-3475f92 e-flex e-con-boxed e-con e-child" data-id="3475f92" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-d3589a3 elementor-widget elementor-widget-heading" data-id="d3589a3" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Other Services</h3> </div> </div> <div class="elementor-element elementor-element-b2eea0d elementor-widget elementor-widget-text-editor" data-id="b2eea0d" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p><a style="color: #ffcc00;" href="https://ccdb.ca/wp/pricing-genomics/">Click here</a> for the pricing of our genomic and metagenomic barcoding services. </p><p>For more information or to obtain a quote for your project, contact us.</p> </div> </div> <div class="elementor-element elementor-element-e49c1d9 elementor-icon-list--layout-traditional elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list" data-id="e49c1d9" data-element_type="widget" data-widget_type="icon-list.default"> <div class="elementor-widget-container"> <ul class="elementor-icon-list-items"> <li class="elementor-icon-list-item"> <span class="elementor-icon-list-icon"> <svg 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73.4-41.4 132.7-96.3 142.8-104.7 173.4-128.7 5.8-4.5 9.2-11.5 9.2-18.9v-19c0-26.5-21.5-48-48-48H48C21.5 64 0 85.5 0 112v19c0 7.4 3.4 14.3 9.2 18.9 30.6 23.9 40.7 32.4 173.4 128.7 16.8 12.2 50.2 41.8 73.4 41.4z"></path></svg> </span> <span class="elementor-icon-list-text">info@ccdb.ca</span> </a> </li> <li class="elementor-icon-list-item"> <span class="elementor-icon-list-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-fas-hourglass-half" viewBox="0 0 384 512" xmlns="http://www.w3.org/2000/svg"><path d="M360 0H24C10.745 0 0 10.745 0 24v16c0 13.255 10.745 24 24 24 0 90.965 51.016 167.734 120.842 192C75.016 280.266 24 357.035 24 448c-13.255 0-24 10.745-24 24v16c0 13.255 10.745 24 24 24h336c13.255 0 24-10.745 24-24v-16c0-13.255-10.745-24-24-24 0-90.965-51.016-167.734-120.842-192C308.984 231.734 360 154.965 360 64c13.255 0 24-10.745 24-24V24c0-13.255-10.745-24-24-24zm-75.078 384H99.08c17.059-46.797 52.096-80 92.92-80 40.821 0 75.862 33.196 92.922 80zm.019-256H99.078C91.988 108.548 88 86.748 88 64h208c0 22.805-3.987 44.587-11.059 64z"></path></svg> </span> <span class="elementor-icon-list-text">Monday – Friday<br />8:30 am to 4:00 pm</span> </li> </ul> </div> </div> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-4fff5777 e-flex e-con-boxed e-con e-parent" data-id="4fff5777" data-element_type="container" data-settings="{"background_background":"classic"}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-c30782d e-con-full e-flex e-con e-child" data-id="c30782d" data-element_type="container" data-settings="{"background_background":"classic","animation":"none","animation_delay":600}"> <div class="elementor-element elementor-element-94d00e3 e-flex e-con-boxed e-con e-child" data-id="94d00e3" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-319970c elementor-widget elementor-widget-heading" data-id="319970c" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Price Categories</h3> </div> </div> <div class="elementor-element elementor-element-8db712f elementor-widget elementor-widget-heading" data-id="8db712f" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h4 class="elementor-heading-title elementor-size-default">Standard Rate</h4> </div> </div> <div class="elementor-element elementor-element-894b825 elementor-widget elementor-widget-text-editor" data-id="894b825" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>The full standard rate will apply to all submissions unless the Provider qualifies for the discounted research rate (see below).</p> </div> </div> <div class="elementor-element elementor-element-d3c7b46 elementor-widget elementor-widget-heading" data-id="d3c7b46" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h4 class="elementor-heading-title elementor-size-default">Research Rate</h4> </div> </div> <div class="elementor-element elementor-element-d70dff6 elementor-widget elementor-widget-text-editor" data-id="d70dff6" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>This discounted rate is available to Providers that align their research efforts with our mission to assemble a DNA barcode library for all eukaryotic species. This research rate applies only to submissions where all required data elements are uploaded to BOLD.</p> </div> </div> <div class="elementor-element elementor-element-d29a81c elementor-widget elementor-widget-text-editor" data-id="d29a81c" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>To qualify, Providers must conform to the following metadata quality standards: collection location with GPS co-ordinates, voucher specimen images (representative images are not acceptable, for more information please refer to the Photography Guide in the <a href="https://boldsystems.org/resource-hub/documentation/" target="_blank" rel="noopener">BOLD Handbook</a>), museum voucher location and ID and full taxonomy to the level of species or operational taxonomic unit. </p> </div> </div> <div class="elementor-element elementor-element-589d6c0 elementor-widget elementor-widget-text-editor" data-id="589d6c0" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>The Provider also agrees to data sharing <span class="ui-provider ef bbd bbe bbf bbg bbh bbi bbj bbk bbl bbm bbn bbo bbp bbq bbr bbs bbt bbu bbv bbw bbx bby bbz bca bcb bcc bcd bce bcf bcg bch bci bcj bck" dir="ltr">in compliance with best practices established under CBG’s Data Release Policy as dictated by funding agencies. Subsequent to these requirements, records will be released publicly upon data publication, or after 12 months from the date the sequence was generated, whichever comes sooner</span>.</p> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-7fc144e e-con-full e-flex e-con e-child" data-id="7fc144e" data-element_type="container"> <div class="elementor-element elementor-element-f845b62 elementor-widget elementor-widget-heading" data-id="f845b62" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Analytical Services</h3> </div> </div> <div class="elementor-element elementor-element-03ac244 elementor-widget elementor-widget-heading" data-id="03ac244" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h4 class="elementor-heading-title elementor-size-default">High-throughput barcode analysis (96-well plate/box)</h4> </div> </div> <div class="elementor-element elementor-element-11c0c24 elementor-widget elementor-widget-text-editor" data-id="11c0c24" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>This represents the standard package to which additional services can be added. Please note that all submissions must follow the 96-well microplate guidelines available on our website. This high-throughput barcode analysis is available for animals (COI), plants (matK+rbcL), and fungi (ITS) and includes the following services: Metadata validation, DNA extraction, PCR amplification, bidirectional sequencing of barcode regions(s), sequence assembly and base calling, and sequence and trace file upload to the Barcode of Life Data System (BOLD).</p> </div> </div> <div class="elementor-element elementor-element-dd194ad elementor-widget__width-inherit elementor-widget elementor-widget-eael-adv-accordion" data-id="dd194ad" data-element_type="widget" data-widget_type="eael-adv-accordion.default"> <div class="elementor-widget-container"> <div class="eael-adv-accordion" id="eael-adv-accordion-dd194ad" data-scroll-on-click="no" data-scroll-speed="300" data-accordion-id="dd194ad" data-accordion-type="toggle" data-toogle-speed="300"> <div class="eael-accordion-list"> <div id="animal-tissue-single-pass-only-starting-at-1250" class="elementor-tab-title eael-accordion-header" tabindex="0" data-tab="1" aria-controls="elementor-tab-content-2311"><span class="eael-advanced-accordion-icon-closed"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-plus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-advanced-accordion-icon-opened"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-minus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-accordion-tab-title">Animal tissue (single pass only) – starting at $1,250</span></div><div id="elementor-tab-content-2311" class="eael-accordion-content clearfix" data-tab="1" aria-labelledby="animal-tissue-single-pass-only-starting-at-1250"><p>Single pass barcode analysis of animal tissue is recommended for samples that are expected to yield high quality DNA. This service will involve single round of PCR with universal primers targeting full barcode region (658bp or similar length), followed by PCR check, and bidirectional sequencing. Quality of the provided material will impact the results and for poorly preserved and/or old specimens with degraded DNA additional analysis may be recommended. Results are normally available within 4-6 weeks from the date the lab begins processing of samples. Lead time depends on the size of the queue and can be extended due to missing or incomplete specimen data and/or payment delays.</p><p><strong>Standard Rate: $2,200<br />Research Rate: $1,250</strong></p></div> </div><div class="eael-accordion-list"> <div id="animal-tissue-failure-tracking-optional-starting-at-875" class="elementor-tab-title eael-accordion-header" tabindex="0" data-tab="2" aria-controls="elementor-tab-content-2312"><span class="eael-advanced-accordion-icon-closed"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-plus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-advanced-accordion-icon-opened"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-minus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-accordion-tab-title">Animal tissue (failure-tracking, optional) – starting at $875</span></div><div id="elementor-tab-content-2312" class="eael-accordion-content clearfix" data-tab="2" aria-labelledby="animal-tissue-failure-tracking-optional-starting-at-875"><p>Some samples may fail to amplify and/or sequence due to random primer mismatches or degraded material. Revisiting failed samples with primer sets that target shorter overlapping fragments spanning the barcode region enables barcode recovery. This option delays processing time significantly as the analysis must be completed in multiple stages. Allow additional 2-4 weeks to complete processing of samples.</p><p><strong>Standard Rate: $1,540<br />Research Rate: $875</strong></p></div> </div><div class="eael-accordion-list"> <div id="animal-tissue-first-pass-with-failure-tracking-starting-at-2125" class="elementor-tab-title eael-accordion-header" tabindex="0" data-tab="3" aria-controls="elementor-tab-content-2313"><span class="eael-advanced-accordion-icon-closed"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-plus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-advanced-accordion-icon-opened"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-minus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-accordion-tab-title">Animal tissue (first pass with failure-tracking) – starting at $2,125</span></div><div id="elementor-tab-content-2313" class="eael-accordion-content clearfix" data-tab="3" aria-labelledby="animal-tissue-first-pass-with-failure-tracking-starting-at-2125"><p>Single pass barcode analysis of animal tissue is recommended for samples that are expected to yield high quality DNA. This service will involve single round of PCR with universal primers targeting full barcode region (658bp or similar length), followed by PCR check, and bidirectional sequencing. Quality of the provided material will impact the results and for poorly preserved and/or old specimens with degraded DNA additional analysis may be recommended. Results are normally available within 4-6 weeks from the date the lab begins processing of samples. Lead time depends on the size of the queue and can be extended due to missing or incomplete specimen data and/or payment delays.</p><p>Some samples may fail to amplify and/or sequence due to random primer mismatches or degraded material. Revisiting failed samples with primer sets that target shorter overlapping fragments spanning the barcode region enables barcode recovery. This option delays processing time significantly as the analysis must be completed in multiple stages. Allow additional 2-4 weeks to complete processing of samples.</p><p><strong>Standard Rate: $3,750<br />Research Rate: $2,125</strong></p></div> </div><div class="eael-accordion-list"> <div id="animal-tissue-older-andor-degraded-material-starting-at-1750" class="elementor-tab-title eael-accordion-header" tabindex="0" data-tab="4" aria-controls="elementor-tab-content-2314"><span class="eael-advanced-accordion-icon-closed"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-plus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-advanced-accordion-icon-opened"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-minus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-accordion-tab-title">Animal tissue (older and/or degraded material) – starting at $1,750</span></div><div id="elementor-tab-content-2314" class="eael-accordion-content clearfix" data-tab="4" aria-labelledby="animal-tissue-older-andor-degraded-material-starting-at-1750"><p>Recommended for submissions with mostly old specimens that are not likely to produce amplicons for full barcode region. This option allows use of failure-tracking option as the initial step resulting in reduced processing time. Results are normally available within 4-6 weeks from the date the lab begins processing of samples.</p><p><strong>Standard Rate: $3,100<br />Research Rate: $1,750</strong></p></div> </div><div class="eael-accordion-list"> <div id="plant-tissue-starting-at-1800" class="elementor-tab-title eael-accordion-header" tabindex="0" data-tab="5" aria-controls="elementor-tab-content-2315"><span class="eael-advanced-accordion-icon-closed"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-plus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-advanced-accordion-icon-opened"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-minus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-accordion-tab-title">Plant tissue – starting at $1,800</span></div><div id="elementor-tab-content-2315" class="eael-accordion-content clearfix" data-tab="5" aria-labelledby="plant-tissue-starting-at-1800"><p>This offer applies to plant tissues submitted in 96-tube racks. This option includes analysis of two markers (barcode standard for plants) with bidirectional sequencing, sequence editing and submission of traces and consensus sequences to BOLD. Results are normally available within 4-6 weeks from the date the lab begins processing of samples.</p><p><strong>Standard Rate: $3,300<br />Research Rate: $1,800</strong></p></div> </div><div class="eael-accordion-list"> <div id="fungal-and-algal-tissue-starting-at-1500" class="elementor-tab-title eael-accordion-header" tabindex="0" data-tab="6" aria-controls="elementor-tab-content-2316"><span class="eael-advanced-accordion-icon-closed"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-plus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H272V64c0-17.67-14.33-32-32-32h-32c-17.67 0-32 14.33-32 32v144H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h144v144c0 17.67 14.33 32 32 32h32c17.67 0 32-14.33 32-32V304h144c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-advanced-accordion-icon-opened"><svg aria-hidden="true" class="fa-accordion-icon e-font-icon-svg e-fas-minus" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M416 208H32c-17.67 0-32 14.33-32 32v32c0 17.67 14.33 32 32 32h384c17.67 0 32-14.33 32-32v-32c0-17.67-14.33-32-32-32z"></path></svg></span><span class="eael-accordion-tab-title">Fungal and algal tissue – starting at $1,500</span></div><div id="elementor-tab-content-2316" class="eael-accordion-content clearfix" data-tab="6" aria-labelledby="fungal-and-algal-tissue-starting-at-1500"><p>This offer applies to fungal and algal specimens submitted in 96-tube racks. This option includes analysis of single loci (barcode standard for fungi or algae) with bidirectional sequencing, sequence editing and submission of traces and consensus sequences to BOLD. Results are normally available within 4-6 weeks from the date the lab begins processing of samples.</p><p><strong>Standard Rate: $2,640<br />Research Rate: $1,500</strong></p></div> </div></div> </div> </div> <div class="elementor-element elementor-element-efb2e9a elementor-widget elementor-widget-heading" data-id="efb2e9a" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h4 class="elementor-heading-title elementor-size-default">SMRT Sequencing on PacBio Sequel IIe</h4> </div> </div> <div class="elementor-element elementor-element-ae591d4 elementor-widget elementor-widget-text-editor" data-id="ae591d4" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>This high-throughput barcoding protocol includes DNA extraction from fresh tissue samples (or whole vouchers for small organisms) in a single batch of 96-well plates. Freshly extracted DNA is used as template for PCR amplification in 384-well format with CLepFol primer cocktails thus material must be fresh (up to 5 years). This works well for most insects and other terrestrial arthropods. As the produced amplicons require pooling prior to SMRT sequencing workflow, each amplicon is dual-indexed with a unique pair of molecular tags (unique molecular identifier, UMI) on both 5′- and 3′-ends. The position of a sample in a 96-well plate and the place each 96-well plate occupies in the batch is used to link the unique pairs of UMIs in the resulting SMRT sequences to the source sample. The analysis of circular consensus sequences is supported by mBrave followed by validation and submission of results to BOLD.</p> </div> </div> <div class="elementor-element elementor-element-6a3cb9d elementor-widget elementor-widget-text-editor" data-id="6a3cb9d" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Because the indexing PCR requires the production of template-free PCR plates in large batches and in sets of <strong>24</strong> 384 plates, and due to complexity of the workflow which involves five processing stages that collectively include 30 steps, we can only accept samples in batches of <strong>48</strong> or <strong>96</strong> 96-well plates. The price per sample varies based on the number of samples in a single batch (4560 or 9120) or DNA extraction protocol requested (SPRI or GFP).</p> </div> </div> <div class="elementor-element elementor-element-dd5e5fb uael-header-sticky-no uael-border-yes elementor-widget elementor-widget-uael-table" data-id="dd5e5fb" data-element_type="widget" data-widget_type="uael-table.default"> <div class="elementor-widget-container"> <div itemscope class="uael-table-wrapper" itemtype="http://schema.org/Table"> <table id="uael-table-id-dd5e5fb" class="uael-text-break uael-column-rules uael-table" data-sort-table="no" data-show-entry="no" data-searchable="no" data-responsive="yes"> <thead> <tr class="uael-table-row"> <th data-sort="0" class="sort-this elementor-repeater-item-dcb4d40 uael-table-col uael-table-head-cell-text" scope="col"> <span class="sort-style"> <span class="uael-table__text"> <span class="uael-table__text-inner">Batch Analysis with SMRT Sequencing</span> </span> </span> </th> <th data-sort="1" class="sort-this elementor-repeater-item-3e8233c uael-table-col uael-table-head-cell-text" scope="col"> <span class="sort-style"> <span class="uael-table__text"> <span class="uael-table__text-inner">SPRI Extraction</span> </span> </span> </th> <th data-sort="2" class="sort-this elementor-repeater-item-802588c uael-table-col uael-table-head-cell-text" scope="col"> <span class="sort-style"> <span class="uael-table__text"> <span class="uael-table__text-inner">GFP Extraction</span> </span> </span> </th> </thead> <tbody>  <tr data-entry="1" class="uael-table-row"> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-cfcc158" data-title="Batch Analysis with SMRT Sequencing"> <span class="uael-table__text"> <span class="uael-table__text-inner"><strong>48</strong> 96-well plates (fresh material, insects only)</span> </span> </td> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-043d388" data-title="SPRI Extraction"> <span class="uael-table__text"> <span class="uael-table__text-inner">$17,100.00</span> </span> </td> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-d9f2c42" data-title="GFP Extraction"> <span class="uael-table__text"> <span class="uael-table__text-inner">$22,800.00</span> </span> </td> </tr><tr data-entry="2" class="uael-table-row"> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-b6cf936" data-title="Batch Analysis with SMRT Sequencing"> <span class="uael-table__text"> <span class="uael-table__text-inner"><strong>96</strong> 96-well plates (fresh material, insects only)</span> </span> </td> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-7df5662" data-title="SPRI Extraction"> <span class="uael-table__text"> <span class="uael-table__text-inner">$27,360.00</span> </span> </td> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-4e891b0" data-title="GFP Extraction"> <span class="uael-table__text"> <span class="uael-table__text-inner">$38,760.00</span> </span> </td> </tr><tr data-entry="3" class="uael-table-row"> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-f6d6411" data-title="Batch Analysis with SMRT Sequencing"> <span class="uael-table__text"> <span class="uael-table__text-inner"><strong>Single</strong> 96-well plate, NGSFT protocol for samples with degraded DNA (decades-old insects)</span> </span> </td> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-f1715c6" data-title="SPRI Extraction"> <span class="uael-table__text"> <span class="uael-table__text-inner">N/A</span> </span> </td> <td class="uael-table-col uael-table-body-cell-text elementor-repeater-item-37a78a6" data-title="GFP Extraction"> <span class="uael-table__text"> <span class="uael-table__text-inner">$3,500.00</span> </span> </td> </tbody> </table> </div> </div> </div> <div class="elementor-element elementor-element-1ff6257 elementor-widget elementor-widget-heading" data-id="1ff6257" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h4 class="elementor-heading-title elementor-size-default">SPRI vs GFP DNA Extraction</h4> </div> </div> <div class="elementor-element elementor-element-2521ae6 elementor-widget elementor-widget-text-editor" data-id="2521ae6" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>CCDB introduced a SPRI DNA extraction protocol in 2019 as an alternative to the glass-fiber extraction method. While the SPRI method is more scalable, it is not suited for older material or samples with high polysaccharide contents (e.g. mollusks or echinoderms). As well, it does not tolerate oversampling so it is crucial to follow the sample submission requirements. Oversampling will result in poor DNA extraction and a subsequent failure to amplify the COI barcode region even in fresh specimens.</p> </div> </div> <div class="elementor-element elementor-element-b458fdd elementor-widget elementor-widget-text-editor" data-id="b458fdd" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>The GFP method involves decade-proven automated CCDB glass fiber membrane protocol as a fail-proof alternative to the more sensitive SPRI method. Due to capacity constraints and higher consumable and labour cost, it is a more expensive option with longer pre-sequencing processing time.</p> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-d7134bf e-flex e-con-boxed e-con e-parent" data-id="d7134bf" data-element_type="container" data-settings="{"background_background":"classic"}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-c484cc7 e-flex e-con-boxed e-con e-child" data-id="c484cc7" data-element_type="container" data-settings="{"background_background":"classic"}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-daf6651 e-con-full e-flex e-con e-child" data-id="daf6651" data-element_type="container"> <div class="elementor-element elementor-element-1f11da0 elementor-widget elementor-widget-heading" data-id="1f11da0" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 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