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class="vector-body" aria-labelledby="firstHeading" data-mw-ve-target-container> <div class="vector-body-before-content"> <div class="mw-indicators"> </div> <div id="siteSub" class="noprint">From Wikipedia, the free encyclopedia</div> </div> <div id="contentSub"><div id="mw-content-subtitle"><span class="mw-redirectedfrom">(Redirected from <a href="/w/index.php?title=Trichrome_stain&redirect=no" class="mw-redirect" title="Trichrome stain">Trichrome stain</a>)</span></div></div> <div id="mw-content-text" class="mw-body-content"><div class="mw-content-ltr mw-parser-output" lang="en" dir="ltr"><div class="shortdescription nomobile noexcerpt noprint searchaux" style="display:none">Histological staining method</div> <style data-mw-deduplicate="TemplateStyles:r1236090951">.mw-parser-output .hatnote{font-style:italic}.mw-parser-output div.hatnote{padding-left:1.6em;margin-bottom:0.5em}.mw-parser-output .hatnote i{font-style:normal}.mw-parser-output .hatnote+link+.hatnote{margin-top:-0.5em}@media print{body.ns-0 .mw-parser-output .hatnote{display:none!important}}</style><div role="note" class="hatnote navigation-not-searchable"><span>"Trichrome" redirects here. For three color vision, see <a href="/wiki/Trichromacy" title="Trichromacy">Trichromacy</a>. For the band and album, see <a href="/wiki/Trichromes" title="Trichromes">Trichromes</a>. For three-color systems, see <a href="/wiki/CMY_color_model" class="mw-redirect" title="CMY color model">CMY color model</a>, <a href="/wiki/RGB_color_model" title="RGB color model">RGB color model</a>, and <a href="/wiki/RYB_color_model" title="RYB color model">RYB color model</a>.</span> <span>Not to be confused with <a href="/wiki/Trichome" title="Trichome">Trichome</a>.</span></div> <style data-mw-deduplicate="TemplateStyles:r1251242444">.mw-parser-output .ambox{border:1px solid #a2a9b1;border-left:10px solid #36c;background-color:#fbfbfb;box-sizing:border-box}.mw-parser-output .ambox+link+.ambox,.mw-parser-output .ambox+link+style+.ambox,.mw-parser-output .ambox+link+link+.ambox,.mw-parser-output .ambox+.mw-empty-elt+link+.ambox,.mw-parser-output .ambox+.mw-empty-elt+link+style+.ambox,.mw-parser-output .ambox+.mw-empty-elt+link+link+.ambox{margin-top:-1px}html body.mediawiki .mw-parser-output .ambox.mbox-small-left{margin:4px 1em 4px 0;overflow:hidden;width:238px;border-collapse:collapse;font-size:88%;line-height:1.25em}.mw-parser-output .ambox-speedy{border-left:10px solid #b32424;background-color:#fee7e6}.mw-parser-output .ambox-delete{border-left:10px solid #b32424}.mw-parser-output .ambox-content{border-left:10px solid #f28500}.mw-parser-output .ambox-style{border-left:10px solid #fc3}.mw-parser-output .ambox-move{border-left:10px solid #9932cc}.mw-parser-output .ambox-protection{border-left:10px solid #a2a9b1}.mw-parser-output .ambox .mbox-text{border:none;padding:0.25em 0.5em;width:100%}.mw-parser-output .ambox .mbox-image{border:none;padding:2px 0 2px 0.5em;text-align:center}.mw-parser-output .ambox .mbox-imageright{border:none;padding:2px 0.5em 2px 0;text-align:center}.mw-parser-output .ambox .mbox-empty-cell{border:none;padding:0;width:1px}.mw-parser-output .ambox .mbox-image-div{width:52px}@media(min-width:720px){.mw-parser-output .ambox{margin:0 10%}}@media print{body.ns-0 .mw-parser-output .ambox{display:none!important}}</style><table class="box-More_footnotes_needed plainlinks metadata ambox ambox-style ambox-More_footnotes_needed" role="presentation"><tbody><tr><td class="mbox-image"><div class="mbox-image-div"><span typeof="mw:File"><span><img alt="" src="//upload.wikimedia.org/wikipedia/commons/thumb/a/a4/Text_document_with_red_question_mark.svg/40px-Text_document_with_red_question_mark.svg.png" decoding="async" width="40" height="40" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/a/a4/Text_document_with_red_question_mark.svg/60px-Text_document_with_red_question_mark.svg.png 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/a/a4/Text_document_with_red_question_mark.svg/80px-Text_document_with_red_question_mark.svg.png 2x" data-file-width="48" data-file-height="48" /></span></span></div></td><td class="mbox-text"><div class="mbox-text-span">This article includes a list of <a href="/wiki/Wikipedia:Citing_sources#General_references" title="Wikipedia:Citing sources">general references</a>, but <b>it lacks sufficient corresponding <a href="/wiki/Wikipedia:Citing_sources#Inline_citations" title="Wikipedia:Citing sources">inline citations</a></b>.<span class="hide-when-compact"> Please help to <a href="/wiki/Wikipedia:WikiProject_Reliability" title="Wikipedia:WikiProject Reliability">improve</a> this article by <a href="/wiki/Wikipedia:When_to_cite" title="Wikipedia:When to cite">introducing</a> more precise citations.</span> <span class="date-container"><i>(<span class="date">July 2017</span>)</i></span><span class="hide-when-compact"><i> (<small><a href="/wiki/Help:Maintenance_template_removal" title="Help:Maintenance template removal">Learn how and when to remove this message</a></small>)</i></span></div></td></tr></tbody></table> <p> <b>Trichrome staining</b> is a <a href="/wiki/Histological" class="mw-redirect" title="Histological">histological</a> <a href="/wiki/Staining" title="Staining">staining</a> method that uses two or more <a href="/wiki/Acid_dye" title="Acid dye">acid dyes</a> in conjunction with a <a href="/wiki/Polyacid" class="mw-redirect" title="Polyacid">polyacid</a>. Staining differentiates tissues by tinting them in contrasting colours. It increases the contrast of microscopic features in cells and <a href="/wiki/Tissue_(biology)" title="Tissue (biology)">tissues</a>, which makes them easier to see when viewed through a <a href="/wiki/Microscope" title="Microscope">microscope</a>. </p><p>The word <i><a href="https://en.wiktionary.org/wiki/trichrome#English" class="extiw" title="wikt:trichrome">trichrome</a></i> means "three colours". The first staining protocol that was described as "trichrome" was <a href="/wiki/Mallory%27s_trichrome_stain" title="Mallory's trichrome stain">Mallory's trichrome stain</a>, which differentially stained <a href="/wiki/Erythrocyte" class="mw-redirect" title="Erythrocyte">erythrocytes</a> to a red colour, <a href="/wiki/Muscle_tissue" class="mw-redirect" title="Muscle tissue">muscle tissue</a> to a red colour, and <a href="/wiki/Collagen" title="Collagen">collagen</a> to a blue colour. Some other trichrome staining protocols are the <a href="/wiki/Masson%27s_trichrome_stain" title="Masson's trichrome stain">Masson's trichrome stain</a>, <a href="/wiki/Lillie%27s_trichrome" title="Lillie's trichrome">Lillie's trichrome</a>, and the <a href="/wiki/G%C3%B6m%C3%B6ri_trichrome_stain" title="Gömöri trichrome stain">Gömöri trichrome stain</a>. </p> <meta property="mw:PageProp/toc" /> <div class="mw-heading mw-heading2"><h2 id="Purpose">Purpose</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Trichrome_staining&action=edit&section=1" title="Edit section: Purpose"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>Without trichrome staining, discerning one feature from another can be extremely difficult. <a href="/wiki/Smooth_muscle_tissue" class="mw-redirect" title="Smooth muscle tissue">Smooth muscle tissue</a>, for example, is hard to differentiate from collagen. A trichrome stain can colour the muscle tissue red, and the collagen fibres green or blue. <a href="/wiki/Liver_biopsy" title="Liver biopsy">Liver biopsies</a> may have fine collagen fibres between the liver cells, and the amount of collagen may be estimated based on the staining method. Trichrome methods are now used for differentiating muscle from collagen, pituitary alpha cells from beta cells, fibrin from collagen, and <a href="/wiki/Mitochondria" class="mw-redirect" title="Mitochondria">mitochondria</a> in fresh frozen muscle sections, among other applications. It helps in identifying increases in collagenous tissue (i.e., fibrotic changes) such as in <a href="/wiki/Liver_cirrhosis" class="mw-redirect" title="Liver cirrhosis">liver cirrhosis</a> and distinguishing tumours arising from muscle cells and fibroblasts.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup> </p> <div class="mw-heading mw-heading2"><h2 id="Procedure">Procedure</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Trichrome_staining&action=edit&section=2" title="Edit section: Procedure"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>Trichrome staining techniques employ two or more <a href="/wiki/Acid_dye" title="Acid dye">acid dyes</a>. Normally acid dyes would stain the same basic proteins, but by applying them sequentially the staining pattern can be manipulated. A polyacid (such as <a href="/wiki/Phosphomolybdic_acid" title="Phosphomolybdic acid">phosphomolybdic acid</a> or <a href="/wiki/Tungstophosphoric_acid" class="mw-redirect" title="Tungstophosphoric acid">Phosphotungstic acid</a>) is used to remove dye selectively. Polyacids are thought to behave as dyes with a high <a href="/wiki/Molecular_weight" class="mw-redirect" title="Molecular weight">molecular weight</a>: they displace easily removed dye from collagen.<sup class="noprint Inline-Template Template-Fact" style="white-space:nowrap;">[<i><a href="/wiki/Wikipedia:Citation_needed" title="Wikipedia:Citation needed"><span title="This claim needs references to reliable sources. (February 2021)">citation needed</span></a></i>]</sup> </p><p>Usually a red dye in dilute <a href="/wiki/Acetic_acid" title="Acetic acid">acetic acid</a> is applied first to overstain all components. Then a polyacid is applied to remove the red dye from collagen and some other components by displacement. A second acid dye (blue or green) in dilute acetic acid is applied which, in turn, displaces the polyacid, resulting in collagen stained in a contrasting colour to the initial dye used. If erythrocytes are to be stained, a small molecular weight yellow or orange dye is applied before staining with the red dye. It is usually applied from a saturated solution in 80% ethanol and often in conjunction with picric acid (itself a dye) and a polyacid. The methods exploit minor differences in tissue reaction to dyes, density, accessibility and so on.<sup class="noprint Inline-Template Template-Fact" style="white-space:nowrap;">[<i><a href="/wiki/Wikipedia:Citation_needed" title="Wikipedia:Citation needed"><span title="This claim needs references to reliable sources. (February 2021)">citation needed</span></a></i>]</sup> </p><p>Trichrome stains in which dyes and a polyacid are applied sequentially are called <i>multi-step trichromes</i>. In "one-step" methods, all the dyes—with or without a polyacid—are combined in a single solution. One of the oldest single-step approaches to trichrome staining is van Gieson's method, which stains muscle and <a href="/wiki/Cytoplasm" title="Cytoplasm">cytoplasm</a> yellow, and collagen red. Another is the <a href="/wiki/G%C3%B6m%C3%B6ri_trichrome_stain" title="Gömöri trichrome stain">Gömöri trichrome stain</a>, which closely mimics Masson's trichrome. In "yellowsolve" methods, a red dye in dilute acetic acid is first applied, then the section is very thoroughly dehydrated to ensure that no moisture remains. The red dye is then displaced by a yellow dye in a solvent, such as cellosolve (2-ethoxy-ethanol). The name <i>yellowsolve</i> is a blend of the terms <i>yellow</i> and <i>cellosolve</i>. Lendrum's phloxine-tartrazine for cell inclusions is one example of a yellowsolve stain.<sup class="noprint Inline-Template Template-Fact" style="white-space:nowrap;">[<i><a href="/wiki/Wikipedia:Citation_needed" title="Wikipedia:Citation needed"><span title="This claim needs references to reliable sources. (February 2021)">citation needed</span></a></i>]</sup> </p> <div class="mw-heading mw-heading2"><h2 id="Dyes">Dyes</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Trichrome_staining&action=edit&section=3" title="Edit section: Dyes"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <p>Among the dyes used for trichrome staining are: </p> <dl><dt>Red</dt> <dd><a href="/wiki/Acid_fuchsin" title="Acid fuchsin">Acid fuchsin</a>, xylidine ponceau, chromotrope 2R, <a href="/wiki/Biebrich_scarlet" title="Biebrich scarlet">Biebrich scarlet</a>, ponceau 6R, <a href="/wiki/Phloxine" title="Phloxine">phloxine</a></dd> <dt>Blue and green</dt> <dd>Light green SF yellowish, <a href="/wiki/Fast_Green_FCF" title="Fast Green FCF">Fast Green FCF</a>, <a href="/wiki/Methyl_blue" title="Methyl blue">methyl blue</a>, <a href="/wiki/Water_blue" title="Water blue">water blue</a></dd> <dt>Yellow</dt> <dd><a href="/wiki/Picric_acid" title="Picric acid">Picric acid</a>, <a href="/wiki/Orange_G" title="Orange G">orange G</a>, <a href="/wiki/Martius_yellow" title="Martius yellow">Martius yellow</a>, <a href="/wiki/Tartrazine" title="Tartrazine">tartrazine</a>, milling yellow</dd></dl> <div class="mw-heading mw-heading2"><h2 id="See_also">See also</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Trichrome_staining&action=edit&section=4" title="Edit section: See also"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <ul><li><a href="/wiki/Dispersion_staining" title="Dispersion staining">Dispersion staining</a></li> <li><a href="/wiki/Microscopy" title="Microscopy">Microscopy</a></li> <li><a href="/wiki/Phase-contrast_microscopy" title="Phase-contrast microscopy">Phase-contrast microscopy</a></li></ul> <div class="mw-heading mw-heading2"><h2 id="Notes">Notes</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Trichrome_staining&action=edit&section=5" title="Edit section: Notes"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <style data-mw-deduplicate="TemplateStyles:r1239543626">.mw-parser-output .reflist{margin-bottom:0.5em;list-style-type:decimal}@media screen{.mw-parser-output .reflist{font-size:90%}}.mw-parser-output .reflist .references{font-size:100%;margin-bottom:0;list-style-type:inherit}.mw-parser-output .reflist-columns-2{column-width:30em}.mw-parser-output .reflist-columns-3{column-width:25em}.mw-parser-output .reflist-columns{margin-top:0.3em}.mw-parser-output .reflist-columns ol{margin-top:0}.mw-parser-output .reflist-columns li{page-break-inside:avoid;break-inside:avoid-column}.mw-parser-output .reflist-upper-alpha{list-style-type:upper-alpha}.mw-parser-output .reflist-upper-roman{list-style-type:upper-roman}.mw-parser-output .reflist-lower-alpha{list-style-type:lower-alpha}.mw-parser-output .reflist-lower-greek{list-style-type:lower-greek}.mw-parser-output .reflist-lower-roman{list-style-type:lower-roman}</style><div class="reflist"> <div class="mw-references-wrap"><ol class="references"> <li id="cite_note-1"><span class="mw-cite-backlink"><b><a href="#cite_ref-1">^</a></b></span> <span class="reference-text"><style data-mw-deduplicate="TemplateStyles:r1238218222">.mw-parser-output cite.citation{font-style:inherit;word-wrap:break-word}.mw-parser-output .citation q{quotes:"\"""\"""'""'"}.mw-parser-output .citation:target{background-color:rgba(0,127,255,0.133)}.mw-parser-output .id-lock-free.id-lock-free a{background:url("//upload.wikimedia.org/wikipedia/commons/6/65/Lock-green.svg")right 0.1em center/9px no-repeat}.mw-parser-output .id-lock-limited.id-lock-limited a,.mw-parser-output .id-lock-registration.id-lock-registration a{background:url("//upload.wikimedia.org/wikipedia/commons/d/d6/Lock-gray-alt-2.svg")right 0.1em center/9px no-repeat}.mw-parser-output .id-lock-subscription.id-lock-subscription a{background:url("//upload.wikimedia.org/wikipedia/commons/a/aa/Lock-red-alt-2.svg")right 0.1em center/9px no-repeat}.mw-parser-output .cs1-ws-icon a{background:url("//upload.wikimedia.org/wikipedia/commons/4/4c/Wikisource-logo.svg")right 0.1em center/12px no-repeat}body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-free a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-limited a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-registration a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .id-lock-subscription a,body:not(.skin-timeless):not(.skin-minerva) .mw-parser-output .cs1-ws-icon a{background-size:contain;padding:0 1em 0 0}.mw-parser-output .cs1-code{color:inherit;background:inherit;border:none;padding:inherit}.mw-parser-output .cs1-hidden-error{display:none;color:var(--color-error,#d33)}.mw-parser-output .cs1-visible-error{color:var(--color-error,#d33)}.mw-parser-output .cs1-maint{display:none;color:#085;margin-left:0.3em}.mw-parser-output .cs1-kern-left{padding-left:0.2em}.mw-parser-output .cs1-kern-right{padding-right:0.2em}.mw-parser-output .citation .mw-selflink{font-weight:inherit}@media screen{.mw-parser-output .cs1-format{font-size:95%}html.skin-theme-clientpref-night .mw-parser-output .cs1-maint{color:#18911f}}@media screen and (prefers-color-scheme:dark){html.skin-theme-clientpref-os .mw-parser-output .cs1-maint{color:#18911f}}</style><cite id="CITEREFLamar_Jones" class="citation web cs1">Lamar Jones, M. <a rel="nofollow" class="external text" href="http://www.dako.com/au/index/knowledgecenter/kc_publications/kc_publications_edu/special_stains.htmSpecial_Stains_Education_Guide">"Special Stains Education Guide"</a>. <i>Chapter 10: Mastering the Trichrome Stain</i>. Dako<span class="reference-accessdate">. Retrieved <span class="nowrap">20 May</span> 2011</span>.</cite><span title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=unknown&rft.jtitle=Chapter+10%3A+Mastering+the+Trichrome+Stain&rft.atitle=Special+Stains+Education+Guide&rft.aulast=Lamar+Jones&rft.aufirst=M&rft_id=http%3A%2F%2Fwww.dako.com%2Fau%2Findex%2Fknowledgecenter%2Fkc_publications%2Fkc_publications_edu%2Fspecial_stains.htmSpecial_Stains_Education_Guide&rfr_id=info%3Asid%2Fen.wikipedia.org%3ATrichrome+staining" class="Z3988"></span></span> </li> </ol></div></div> <div class="mw-heading mw-heading2"><h2 id="References">References</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Trichrome_staining&action=edit&section=6" title="Edit section: References"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <ul><li>Baker J.R. (1958) Principles of biological Microtechnique. London: Methuen. (Also paperback reprint, Franklin Classics Trade Press, USA, 2018. ISBN 978-0353331044.)</li> <li>Horobin R.W. (1982) Histochemistry. Stuttgart: Gustav Fischer. ISBN 3437107003.</li> <li>Horobin R.W. (1988) Understanding Histochemistry. Chichester: Ellis Horwood. ISBN 9780470210604.</li> <li>Kiernan J.A. (2015) Histological and Histochemical Methods, 5th ed. Banbury, UK: Scion. ISBN 9781907904325.</li> <li>Prento P. (2009) Staining of macromolecules: possible mechanisms and examples. Biotech. Histochem. 84: 139-158.</li> <li>Puchtler H. Isler H. (1958) The effect of phosphomolybdic acid on the stainability of connective tissues by various dyes. J. Histochem. Cytochem. 6: 265-270.</li> <li>Reid PE. Iagallo M., Nehr S., Jankunis M., Morrow P. (1993) Mechanism of connective tissue techniques. 1. The effect of dye concentration and staining time on anionic dye procedures. Histochem. J. 25: 821-829.</li></ul> <div class="mw-heading mw-heading2"><h2 id="External_links">External links</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Trichrome_staining&action=edit&section=7" title="Edit section: External links"><span>edit</span></a><span class="mw-editsection-bracket">]</span></span></div> <ul><li><a rel="nofollow" class="external text" href="https://www.scribd.com/doc/49699318/Masson-Protocol">Masson's Trichrome Protocol</a></li></ul> <div class="navbox-styles"><style data-mw-deduplicate="TemplateStyles:r1129693374">.mw-parser-output .hlist dl,.mw-parser-output .hlist ol,.mw-parser-output .hlist ul{margin:0;padding:0}.mw-parser-output .hlist dd,.mw-parser-output .hlist dt,.mw-parser-output .hlist li{margin:0;display:inline}.mw-parser-output .hlist.inline,.mw-parser-output .hlist.inline dl,.mw-parser-output .hlist.inline ol,.mw-parser-output .hlist.inline ul,.mw-parser-output .hlist dl dl,.mw-parser-output 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style="padding:0 0.25em"> <ul><li><a href="/wiki/Sudan_stain" title="Sudan stain">Sudan stain</a> <ul><li><a href="/wiki/Sudan_II" title="Sudan II">Sudan II</a></li> <li><a href="/wiki/Sudan_III" title="Sudan III">Sudan III</a></li> <li><a href="/wiki/Sudan_IV" title="Sudan IV">Sudan IV</a></li> <li><a href="/wiki/Oil_Red_O" title="Oil Red O">Oil Red O</a></li> <li><a href="/wiki/Sudan_Black_B" class="mw-redirect" title="Sudan Black B">Sudan Black B</a></li></ul></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Carbohydrate" title="Carbohydrate">Carbohydrates</a></th><td class="navbox-list-with-group navbox-list navbox-odd hlist" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Alcian_blue_stain" title="Alcian blue stain">Alcian blue</a></li> <li><a href="/wiki/Mucicarmine_stain" title="Mucicarmine stain">Mucicarmine</a></li> <li><a href="/wiki/Periodic_acid%E2%80%93Schiff_stain" title="Periodic acid–Schiff stain">Periodic acid–Schiff stain</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Amyloid" title="Amyloid">Amyloid</a></th><td class="navbox-list-with-group navbox-list navbox-even hlist" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Congo_red" title="Congo red">Congo red</a></li> <li><a href="/wiki/Thioflavin" title="Thioflavin">Thioflavin</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Bacteria" title="Bacteria">Bacteria</a></th><td class="navbox-list-with-group navbox-list navbox-odd hlist" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Gram_stain" title="Gram stain">Gram stain</a> <ul><li><a href="/wiki/Methyl_violet" title="Methyl violet">Methyl violet</a>/<a href="/wiki/Crystal_violet" title="Crystal violet">Gentian violet</a></li> <li><a href="/wiki/Safranin" title="Safranin">Safranin</a></li></ul></li> <li><a href="/wiki/Acid-fastness" title="Acid-fastness">Acid-fast</a> <ul><li><a href="/wiki/Ziehl%E2%80%93Neelsen_stain" title="Ziehl–Neelsen stain">Ziehl–Neelsen stain</a>/<a href="/wiki/Kinyoun_stain" title="Kinyoun stain">Kinyoun stain</a> <ul><li><a href="/wiki/Carbol_fuchsin" title="Carbol fuchsin">Carbol fuchsin</a>/<a href="/wiki/Fuchsine" title="Fuchsine">Fuchsine</a></li> <li><a href="/wiki/Methylene_blue" title="Methylene blue">Methylene blue</a></li></ul></li> <li><a href="/wiki/Auramine%E2%80%93rhodamine_stain" title="Auramine–rhodamine stain">Auramine–rhodamine stain</a> <ul><li><a href="/wiki/Auramine_O" title="Auramine O">Auramine O</a></li> <li><a href="/wiki/Rhodamine_B" title="Rhodamine B">Rhodamine B</a></li></ul></li> <li><a href="/wiki/Auramine_phenol_stain" title="Auramine phenol stain">Auramine phenol stain</a></li></ul></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%"><a href="/wiki/Connective_tissue" title="Connective tissue">Connective tissue</a></th><td class="navbox-list-with-group navbox-list navbox-even hlist" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Trichrome_stain" class="mw-redirect" title="Trichrome stain">trichrome stain</a>: <a href="/wiki/Masson%27s_trichrome_stain" title="Masson's trichrome stain">Masson's trichrome stain</a>/<a href="/wiki/Lillie%27s_trichrome" title="Lillie's trichrome">Lillie's trichrome</a> <ul><li><a href="/wiki/Light_Green_SF_yellowish" class="mw-redirect" title="Light Green SF yellowish">Light Green SF yellowish</a></li> <li><a href="/wiki/Biebrich_scarlet" title="Biebrich scarlet">Biebrich scarlet</a></li> <li><a href="/wiki/Phosphomolybdic_acid" title="Phosphomolybdic acid">Phosphomolybdic acid</a></li> <li><a href="/wiki/Fast_Green_FCF" title="Fast Green FCF">Fast Green FCF</a></li> <li><a href="/wiki/Sirius_Red" title="Sirius Red">Sirius Red</a></li></ul></li></ul> <ul><li><a href="/wiki/Van_Gieson%27s_stain" title="Van Gieson's stain">Van Gieson's stain</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Other</th><td class="navbox-list-with-group navbox-list navbox-odd hlist" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Cresyl_violet" title="Cresyl violet">Cresyl violet</a></li> <li><a href="/wiki/Cyanine" title="Cyanine">Cyanine</a></li> <li><a href="/wiki/Jaswant_Singh%E2%80%93Bhattacharji_(JSB)_stain" class="mw-redirect" title="Jaswant Singh–Bhattacharji (JSB) stain">Jaswant Singh–Bhattacharji (JSB) stain</a></li> <li><a href="/wiki/H%26E_stain" title="H&E stain">H&E stain</a> <ul><li><a href="/wiki/Haematoxylin" title="Haematoxylin">Haematoxylin</a></li> <li><a href="/wiki/Eosin_Y" title="Eosin Y">Eosin Y</a></li></ul></li> <li><a href="/wiki/Janus_Green_B" title="Janus Green B">Janus Green B</a></li> <li><a href="/wiki/Giemsa_stain" title="Giemsa stain">Giemsa stain</a></li> <li><a href="/wiki/G%C3%B6m%C3%B6ri_trichrome_stain" title="Gömöri trichrome stain">Gömöri trichrome stain</a></li> <li><a href="/wiki/Luxol_fast_blue_stain" title="Luxol fast blue stain">Luxol fast blue stain</a></li> <li><a href="/wiki/Methyl_blue" title="Methyl blue">Methyl blue</a></li> <li><a href="/wiki/Moeller_stain" title="Moeller stain">Moeller stain</a></li> <li><a href="/wiki/Movat%27s_stain" title="Movat's stain">Movat's stain</a></li> <li><a href="/wiki/Neutral_red" title="Neutral red">Neutral red</a></li> <li><a href="/wiki/Schaeffer%E2%80%93Fulton_stain" title="Schaeffer–Fulton stain">Schaeffer–Fulton stain</a></li> <li><a href="/wiki/Silver_stain" class="mw-redirect" title="Silver stain">Silver stain</a> <ul><li><a href="/wiki/Bielschowsky_stain" title="Bielschowsky stain">Bielschowsky stain</a></li> <li><a href="/wiki/Grocott%27s_methenamine_silver_stain" title="Grocott's methenamine silver stain">Grocott's methenamine silver stain</a></li> <li><a href="/wiki/Warthin%E2%80%93Starry_stain" title="Warthin–Starry stain">Warthin–Starry stain</a></li></ul></li> <li><a href="/wiki/Wright%27s_stain" title="Wright's stain">Wright's stain</a></li></ul> </div></td></tr><tr><th scope="row" class="navbox-group" style="width:1%">Tissue stainability</th><td class="navbox-list-with-group navbox-list navbox-even hlist" style="width:100%;padding:0"><div style="padding:0 0.25em"> <ul><li><a href="/wiki/Acidophile_(histology)" title="Acidophile (histology)">Acidophilic</a></li> <li><a href="/wiki/Basophilic" title="Basophilic">Basophilic</a></li> <li><a href="/wiki/Chromophobe" class="mw-redirect" title="Chromophobe">Chromophobic</a></li></ul> </div></td></tr></tbody></table></div>    </div><noscript><img src="https://login.wikimedia.org/wiki/Special:CentralAutoLogin/start?type=1x1&useformat=desktop" alt="" width="1" height="1" style="border: none; position: absolute;"></noscript> <div class="printfooter" data-nosnippet="">Retrieved from "<a 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