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is-link">Go</button> </div> </div> </form> </div> </div> <ol class="breathe-horizontal" start="1"> <li class="arxiv-result"> <div class="is-marginless"> <p class="list-title is-inline-block"><a href="https://arxiv.org/abs/2104.04188">arXiv:2104.04188</a> <span> [<a href="https://arxiv.org/pdf/2104.04188">pdf</a>] </span> </p> <div class="tags is-inline-block"> <span class="tag is-small is-link tooltip is-tooltip-top" data-tooltip="Tissues and Organs">q-bio.TO</span> <span class="tag is-small is-grey tooltip is-tooltip-top" data-tooltip="Biological Physics">physics.bio-ph</span> <span class="tag is-small is-grey tooltip is-tooltip-top" data-tooltip="Medical Physics">physics.med-ph</span> </div> <div class="is-inline-block" style="margin-left: 0.5rem"> <div class="tags has-addons"> <span class="tag is-dark is-size-7">doi</span> <span class="tag is-light is-size-7"><a class="" href="https://doi.org/10.1021/acsami.1c00495">10.1021/acsami.1c00495 <i class="fa fa-external-link" aria-hidden="true"></i></a></span> </div> </div> </div> <p class="title is-5 mathjax"> Ammonia-induced Calcium Phosphate Nanostructure: A Potential Assay for Studying Osteoporosis and Bone Metastasis </p> <p class="authors"> <span class="search-hit">Authors:</span> <a href="/search/q-bio?searchtype=author&query=Chen%2C+S">Sijia Chen</a>, <a href="/search/q-bio?searchtype=author&query=Wang%2C+Q">Qiong Wang</a>, <a href="/search/q-bio?searchtype=author&query=Eltit%2C+F">Felipe Eltit</a>, <a href="/search/q-bio?searchtype=author&query=Guo%2C+Y">Yubin Guo</a>, <a href="/search/q-bio?searchtype=author&query=Cox%2C+M">Michael Cox</a>, <a href="/search/q-bio?searchtype=author&query=Wang%2C+R">Rizhi Wang</a> </p> <p class="abstract mathjax"> <span class="has-text-black-bis has-text-weight-semibold">Abstract</span>: <span class="abstract-short has-text-grey-dark mathjax" id="2104.04188v1-abstract-short" style="display: inline;"> Osteoclastic resorption of bone plays a central role in both osteoporosis and bone metastasis. A reliable in vitro assay that simulates osteoclastic resorption in vivo would significantly speed up the process of devel-oping effective therapeutic solutions for those diseases. Here we reported the development of a novel and robust nano-structured calcium phosphate coating with unique functions on th… <a class="is-size-7" style="white-space: nowrap;" onclick="document.getElementById('2104.04188v1-abstract-full').style.display = 'inline'; document.getElementById('2104.04188v1-abstract-short').style.display = 'none';">▽ More</a> </span> <span class="abstract-full has-text-grey-dark mathjax" id="2104.04188v1-abstract-full" style="display: none;"> Osteoclastic resorption of bone plays a central role in both osteoporosis and bone metastasis. A reliable in vitro assay that simulates osteoclastic resorption in vivo would significantly speed up the process of devel-oping effective therapeutic solutions for those diseases. Here we reported the development of a novel and robust nano-structured calcium phosphate coating with unique functions on the track-etched porous mem-brane by using an ammonia-induced mineralization (AiM) technique. The calcium phosphate coating uni-formly covers one side of the PET membrane enabling testing for osteoclastic resorption. The track-etched pores in the PET membrane allow calcium phosphate mineral pins to grow inside, which, on one hand, enhances coating integration with membrane substrate, and on the other hand provides diffusion channels for delivering drugs from the lower chamber of a double-chamber cell culture system. The applications of the processed calcium phosphate coating was first demonstrated as a drug screening device by using alen-dronate, a widely used drug for osteoporosis. It was confirmed that the delivery of alendronate significant-ly decreased both the number of monocyte-differentiated osteoclasts and coating resorption. To demon-strate the application in studying bone metastasis, we delivered PC3 prostate cancer conditioned medium and confirmed that both the differentiation of monocytes into osteoclasts and the osteoclastic resorption of the calcium phosphate coating were significantly enhanced. This novel assay thus provides a new platform for studying osteoclastic activities and assessing drug efficacy in vitro. <a class="is-size-7" style="white-space: nowrap;" onclick="document.getElementById('2104.04188v1-abstract-full').style.display = 'none'; document.getElementById('2104.04188v1-abstract-short').style.display = 'inline';">△ Less</a> </span> </p> <p class="is-size-7"><span class="has-text-black-bis has-text-weight-semibold">Submitted</span> 9 April, 2021; <span class="has-text-black-bis has-text-weight-semibold">originally announced</span> April 2021. </p> <p class="comments is-size-7"> <span class="has-text-black-bis has-text-weight-semibold">Journal ref:</span> ACS Applied Materials & Interfaces Manuscript ID: am-2021-004953.R2 </p> </li> <li class="arxiv-result"> <div class="is-marginless"> <p class="list-title is-inline-block"><a href="https://arxiv.org/abs/2009.08491">arXiv:2009.08491</a> <span> [<a href="https://arxiv.org/pdf/2009.08491">pdf</a>] </span> </p> <div class="tags is-inline-block"> <span class="tag is-small is-link tooltip is-tooltip-top" data-tooltip="Tissues and Organs">q-bio.TO</span> <span class="tag is-small is-grey tooltip is-tooltip-top" data-tooltip="Materials Science">cond-mat.mtrl-sci</span> </div> <div class="is-inline-block" style="margin-left: 0.5rem"> <div class="tags has-addons"> <span class="tag is-dark is-size-7">doi</span> <span class="tag is-light is-size-7"><a class="" href="https://doi.org/10.1016/j.jsb.2020.107606">10.1016/j.jsb.2020.107606 <i class="fa fa-external-link" aria-hidden="true"></i></a></span> </div> </div> </div> <p class="title is-5 mathjax"> Globular structure of the hypermineralized tissue in human femoral neck </p> <p class="authors"> <span class="search-hit">Authors:</span> <a href="/search/q-bio?searchtype=author&query=Wang%2C+Q">Qiong Wang</a>, <a href="/search/q-bio?searchtype=author&query=Tang%2C+T">Tengteng Tang</a>, <a href="/search/q-bio?searchtype=author&query=Cooper%2C+D">David Cooper</a>, <a href="/search/q-bio?searchtype=author&query=Eltit%2C+F">Felipe Eltit</a>, <a href="/search/q-bio?searchtype=author&query=Fratzl%2C+P">Peter Fratzl</a>, <a href="/search/q-bio?searchtype=author&query=Guy%2C+P">Pierre Guy</a>, <a href="/search/q-bio?searchtype=author&query=Wang%2C+R">Rizhi Wang</a> </p> <p class="abstract mathjax"> <span class="has-text-black-bis has-text-weight-semibold">Abstract</span>: <span class="abstract-short has-text-grey-dark mathjax" id="2009.08491v1-abstract-short" style="display: inline;"> Bone becomes more fragile with ageing. Among many structural changes, a thin layer of highly mineralized and brittle tissue covers part of the external surface of the thin femoral neck cortex in older people and has been proposed to increase hip fragility. However, there have been very limited reports on this hypermineralized tissue in the femoral neck, especially on its ultrastructure. Such infor… <a class="is-size-7" style="white-space: nowrap;" onclick="document.getElementById('2009.08491v1-abstract-full').style.display = 'inline'; document.getElementById('2009.08491v1-abstract-short').style.display = 'none';">▽ More</a> </span> <span class="abstract-full has-text-grey-dark mathjax" id="2009.08491v1-abstract-full" style="display: none;"> Bone becomes more fragile with ageing. Among many structural changes, a thin layer of highly mineralized and brittle tissue covers part of the external surface of the thin femoral neck cortex in older people and has been proposed to increase hip fragility. However, there have been very limited reports on this hypermineralized tissue in the femoral neck, especially on its ultrastructure. Such information is critical to understanding both the mineralization process and its contributions to hip fracture. Here, we use multiple advanced techniques to characterize the ultrastructure of the hypermineralized tissue in the neck across various length scales. Synchrotron radiation micro-CT found larger but less densely distributed cellular lacunae in hypermineralized tissue than in lamellar bone. When examined under FIB-SEM, the hypermineralized tissue was mainly composed of mineral globules with sizes varying from submicron to a few microns. Nano-sized channels were present within the mineral globules and oriented with the surrounding organic matrix. Transmission electron microscopy showed the apatite inside globules were poorly crystalline, while those at the boundaries between the globules had well-defined lattice structure with crystallinity similar to the apatite mineral in lamellar bone. No preferred mineral orientation was observed both inside each globule and at the boundaries. Collectively, we conclude based on these new observations that the hypermineralized tissue is non-lamellar and has less organized mineral, which may contribute to the high brittleness of the tissue. <a class="is-size-7" style="white-space: nowrap;" onclick="document.getElementById('2009.08491v1-abstract-full').style.display = 'none'; document.getElementById('2009.08491v1-abstract-short').style.display = 'inline';">△ Less</a> </span> </p> <p class="is-size-7"><span class="has-text-black-bis has-text-weight-semibold">Submitted</span> 17 September, 2020; <span class="has-text-black-bis has-text-weight-semibold">originally announced</span> September 2020. </p> <p class="comments is-size-7"> <span class="has-text-black-bis has-text-weight-semibold">Journal ref:</span> Journal of Structural Biology,Volume 212, Issue 2, 1 November 2020, 107606 </p> </li> </ol> <div class="is-hidden-tablet">  <span class="help" style="display: inline-block;"><a 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