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Moorman</h1><div class="affiliations-container fake-truncate js-profile-affiliations"></div></div></div><div class="sidebar-cta-container"><button class="ds2-5-button hidden profile-cta-button grow js-profile-follow-button" data-broccoli-component="user-info.follow-button" data-click-track="profile-user-info-follow-button" data-follow-user-fname="A." data-follow-user-id="46673754" data-follow-user-source="profile_button" data-has-google="false"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">add</span>Follow</button><button class="ds2-5-button hidden profile-cta-button grow js-profile-unfollow-button" data-broccoli-component="user-info.unfollow-button" data-click-track="profile-user-info-unfollow-button" data-unfollow-user-id="46673754"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">done</span>Following</button></div></div><div class="user-stats-container"><a><div class="stat-container js-profile-followers"><p class="label">Followers</p><p class="data">10</p></div></a><a><div class="stat-container js-profile-followees" data-broccoli-component="user-info.followees-count" data-click-track="profile-expand-user-info-following"><p class="label">Following</p><p class="data">4</p></div></a><a><div class="stat-container js-profile-coauthors" data-broccoli-component="user-info.coauthors-count" data-click-track="profile-expand-user-info-coauthors"><p class="label">Co-authors</p><p class="data">4</p></div></a><span><div class="stat-container"><p class="label"><span class="js-profile-total-view-text">Public Views</span></p><p class="data"><span class="js-profile-view-count"></span></p></div></span></div></div></div><div class="right-panel-container"><div class="user-content-wrapper"><div class="uploads-container" id="social-redesign-work-container"><div class="upload-header"><h2 class="ds2-5-heading-sans-serif-xs">Uploads</h2></div><div class="documents-container backbone-social-profile-documents" style="width: 100%;"><div class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by A. Moorman</h3></div><div class="js-work-strip profile--work_container" data-work-id="111793320"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111793320/The_organization_of_the_histone_genes_in_the_genome_of_Xenopus_laevis"><img alt="Research paper thumbnail of The organization of the histone genes in the genome of Xenopus laevis" class="work-thumbnail" src="https://attachments.academia-assets.com/110043769/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111793320/The_organization_of_the_histone_genes_in_the_genome_of_Xenopus_laevis">The organization of the histone genes in the genome of Xenopus laevis</a></div><div class="wp-workCard_item"><span>Nucleic Acids Research</span><span>, 1981</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bdc8d49ac6979bcc9ef2515129613eff" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110043769,"asset_id":111793320,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110043769/download_file?st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111793320"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111793320"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111793320; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=111793320]").text(description); $(".js-view-count[data-work-id=111793320]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 111793320; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='111793320']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 111793320, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bdc8d49ac6979bcc9ef2515129613eff" } } $('.js-work-strip[data-work-id=111793320]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":111793320,"title":"The organization of the histone genes in the genome of Xenopus laevis","translated_title":"","metadata":{"publisher":"Oxford University Press (OUP)","grobid_abstract":"We have studied the organization of the histone genes in the DNA from several individuals of Xenopus laevis. For that purpose, Southern blots of genomic DNA, that was digested with several restriction enzymes, were hybridized with radioactively labeled DNA fragments from clone Xl-hi-1 (14), containing genes for Xenopus histones H2A, H2B, H3 and H4. In the DNA of all animals that were screened we found a major repeating unit of 14 kilobasepairs , which contains genes for histones H2A, H2B, H3 and H4 (H1 not tested) and is represented up to 30 times in the genome. The orde of the genes in this major repeating unit is: H4-H3-H2A-H2B. This order is different from that in the histone DNA of clone Xl-hi-1, i.e. H3-H4-H2A-H2B. In addition to the genes in the major repeating unit, histone genes are present in unique restriction fragments in numbers that vary from one animal to another. The restriction patterns for the histone genes in these unique fragments were found to be different for all eight Xenopus individuals that were screened. The cloned Xenopus histone gene fragment Xl-hi-1 represents such a unique fragment and is not present in the DNA of each single individual. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107727563"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107727563/Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium"><img alt="Research paper thumbnail of Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium" class="work-thumbnail" src="https://attachments.academia-assets.com/106312535/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107727563/Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium">Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium</a></div><div class="wp-workCard_item"><span>Journal of Molecular and Cellular Cardiology</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6f9a6d7e0d64e0dcb886f24dc3ba7511" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106312535,"asset_id":107727563,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106312535/download_file?st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107727563"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107727563"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107727563; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107727563]").text(description); $(".js-view-count[data-work-id=107727563]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107727563; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107727563']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 107727563, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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In this study we show by in situ hybridisation that about 30% of the volume of cultures of differentiating embryonic stem cells consists of cardiomyocytes. RT-PCR analyses showed that the transcription factors Nkx2.5, Gata4, MeJ2c and Irx4 were expressed at levels in the same order of magnitude as the levels observed in embryonic, neonatal and adult hearts. Atrial natriuretic factor and Connexin40, associated with chamber formation in vivo, are expressed at relatively low levels, similar to those observed at early heart development in vivo. To facilitate the isolation of embryonic stem cell-derived cardiomyocytes, a cell line was constructed by stable transfection of the aminoglycoside phosphotransferase cDNA driven by the cardiac-specific distant upstream part of the Na + /Ca 2+ exchanger promoter. To accomplish single copy integration, the construct was inserted into the hypoxanthine phosphoribosyltransferase locus of HM1 embryonic stem cells by homologous recombination. Cardiac-specific resistance to G418sulphate (neomycin) allowed isolation of a pure population of cardiomyocytes. Genetically selected and unselected cell populations were characterised electrophysiologically using patch clamp. To explore whether clusters of cells have a similar differentiation profile, action potentials were measured in aggregates of differentiating embryonic stem cells, using a new method based on the voltage dependent fluorescent dye di-4-ANEPPS. Both whole-cell recordings using patch clamp and optical measurements with di-4-ANEPPS of the action potential showed that upstroke velocity increases and action potential duration decreases with differentiation time, accompanied by a decrease in action potential interval, suggesting the initiation of the developmental program underlying the formation of chamber myocardium.","publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"Journal of Molecular and Cellular Cardiology","grobid_abstract_attachment_id":106312535},"translated_abstract":null,"internal_url":"https://www.academia.edu/107727563/Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium","translated_internal_url":"","created_at":"2023-10-06T23:53:44.266-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":106312535,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106312535/thumbnails/1.jpg","file_name":"31454_UBA002000996_11.pdf","download_url":"https://www.academia.edu/attachments/106312535/download_file?st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Cardiomyocytes_purified_from_differentia.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106312535/31454_UBA002000996_11-libre.pdf?1696664297=\u0026response-content-disposition=attachment%3B+filename%3DCardiomyocytes_purified_from_differentia.pdf\u0026Expires=1732831241\u0026Signature=EYJmfEJV94Y53-MfpzydvEheUbCncpVGIlQe5CBREhAEIABChvt2Dqu-Ns~AivUzCcrkGnFdxgrCik5buFGZ5z0n3JZhPDqng-d4A0HK~2b6lS6Z~c9V1qX0C7zW59c1zHZQqNiZEa9Deq-k5ulw0qUbLKTwBGEDVJdISuf6T~b-Fhf6xpEUecaNkvemeNGPa8yXiQCxkHHoo8vRHt-v6i~9XZtzpvbG4cfwA-lx7rVUPqNidSVTqbog5FkOhaGqdV7AZZDX0rKLp-mNO-uNrSF-ZC5gjmGOoo4Q23sB7Vvd9Fyw7UamMCsXEF~pEOlFMypm5zx4p530Fg8ZmuX~Aw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium","translated_slug":"","page_count":25,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="106780102"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/106780102/Heterogeneous_expression_of_cholesterol_7_alpha_hydroxylase_and_sterol_27_hydroxylase_genes_in_the_rat_liver_lobulus"><img alt="Research paper thumbnail of Heterogeneous expression of cholesterol 7 alpha-hydroxylase and sterol 27-hydroxylase genes in the rat liver lobulus" class="work-thumbnail" src="https://attachments.academia-assets.com/105788947/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/106780102/Heterogeneous_expression_of_cholesterol_7_alpha_hydroxylase_and_sterol_27_hydroxylase_genes_in_the_rat_liver_lobulus">Heterogeneous expression of cholesterol 7 alpha-hydroxylase and sterol 27-hydroxylase genes in the rat liver lobulus</a></div><div class="wp-workCard_item"><span>Journal of Clinical Investigation</span><span>, 1995</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6b5c0819d66b41eb49122a0722dfcc22" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":105788947,"asset_id":106780102,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/105788947/download_file?st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="106780102"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="106780102"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 106780102; 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Enzyme activity, mRNA, and gene transcription of cholesterol 7c-hydroxylase were predominant in pericentral hepatocytes of control rats, being 7.9-, 9.9-, and 4.4-fold higher than in periportal hepatocytes, respectively. Similar localization was found for sterol 27-hydroxylase: 2.9-, 2.5-, and 1.7-fold higher enzyme activity, mRNA, and gene transcription, respectively, was found in pericentral hepatocytes. Interruption of the enterohepatic circulation with colestid resulted in upregulation of these parameters for both enzymes, as a consequence of stimulated gene expression mainly in the periportal zone. In contrast, mRNA levels and gene transcription of 3-hydroxy-3-methylglutaryl CoA reductase showed opposite lobular distribution. Selective periportal expression for the latter was enhanced, but remained local, after colestid treatment. In situ hybridization showed unambiguously that cholesterol 7a-hydroxylase mRNA is localized exclusively in the pericentral zone and that sterol 27-hydroxylase mRNA is expressed preferentially in the pericentral region, though less pronounced. Administration of colestid led to expression of both genes within a larger area of the liver lobulus. In conclusion, we suggest that cholesterol 7a-hydroxylase and sterol 27-hydroxylase are coordinately regulated by the bile acid gradient over the lobulus, resulting in predominant expression in the pericentral zone. 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Potential repair by cell grafting or mobilizing endogenous cells is of particular interest for possible treatments for heart disease, where the poor capacity for cardiomyocyte proliferation probably contributes to the irreversibility of heart failure. Knowledge of the molecular mechanisms that underly formation of heart muscle cells might provide opportunities to repair the diseased heart by induction of (trans) differentiation of endogenous or exogenous cells into heart muscle cells. We briefly review the molecular mechanisms involved in early development of the linear heart tube by differentiation of mesodermal cells into heart muscle cells. Because the initial heart tube does not comprise all the cardiac compartments present in the adult heart, heart muscle cells are added to the distal borders of the tube and within the tube. At both distal borders, mesodermal cell are recruited into the cardiac lineage and, within the heart tube, muscular septa are formed. In this review, the relative late additions of heart muscle cells to the linear heart tube are described and the potential underlying molecular mechanisms are discussed.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021647"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021647"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021647; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50021647]").text(description); $(".js-view-count[data-work-id=50021647]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50021647; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50021647']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50021647, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=50021647]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50021647,"title":"Making more heart muscle","translated_title":"","metadata":{"abstract":"Postnatally, heart muscle cells almost completely lose their ability to divide, which makes their loss after trauma irreversible. 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In this review, the relative late additions of heart muscle cells to the linear heart tube are described and the potential underlying molecular mechanisms are discussed.","publisher":"Wiley-Blackwell","publication_date":{"day":null,"month":null,"year":2004,"errors":{}},"publication_name":"BioEssays"},"translated_abstract":"Postnatally, heart muscle cells almost completely lose their ability to divide, which makes their loss after trauma irreversible. Potential repair by cell grafting or mobilizing endogenous cells is of particular interest for possible treatments for heart disease, where the poor capacity for cardiomyocyte proliferation probably contributes to the irreversibility of heart failure. Knowledge of the molecular mechanisms that underly formation of heart muscle cells might provide opportunities to repair the diseased heart by induction of (trans) differentiation of endogenous or exogenous cells into heart muscle cells. We briefly review the molecular mechanisms involved in early development of the linear heart tube by differentiation of mesodermal cells into heart muscle cells. Because the initial heart tube does not comprise all the cardiac compartments present in the adult heart, heart muscle cells are added to the distal borders of the tube and within the tube. At both distal borders, mesodermal cell are recruited into the cardiac lineage and, within the heart tube, muscular septa are formed. In this review, the relative late additions of heart muscle cells to the linear heart tube are described and the potential underlying molecular mechanisms are discussed.","internal_url":"https://www.academia.edu/50021647/Making_more_heart_muscle","translated_internal_url":"","created_at":"2021-07-17T10:14:14.018-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Making_more_heart_muscle","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="50021646"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/50021646/Developmental_and_hormonal_regulation_of_carbamoyl_phosphate_synthase_gene_expression_in_rat_liver_Evidence_for_control_mechanisms_at_different_levels_in_the_perinatal_period"><img alt="Research paper thumbnail of Developmental and hormonal regulation of carbamoyl-phosphate synthase gene expression in rat liver: Evidence for control mechanisms at different levels in the perinatal period" class="work-thumbnail" src="https://attachments.academia-assets.com/68161936/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/50021646/Developmental_and_hormonal_regulation_of_carbamoyl_phosphate_synthase_gene_expression_in_rat_liver_Evidence_for_control_mechanisms_at_different_levels_in_the_perinatal_period">Developmental and hormonal regulation of carbamoyl-phosphate synthase gene expression in rat liver: Evidence for control mechanisms at different levels in the perinatal period</a></div><div class="wp-workCard_item"><span>Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression</span><span>, 1986</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1d6019f14d391c143f190d1a794bd47b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":68161936,"asset_id":50021646,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/68161936/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021646"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021646"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021646; 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In addition, immunohistochemistry shows that under these conditions the distribution of carbamoylphosphate synthetase is expanded over the entire liver acinus, whereas that of glutamine synthetase is reduced to very few cells bordering the central (terminal hepatic) veins. Using a newly isolated cDNA complementary to rat liver glutamine synthetase mRNA, we show that this regulation is primarily effected at a pretranslational level. (For data on carbamoylphosphate synthetase mRNA levels, see De Groot et al. (1986) Biochim. Biophys. Acta 866, 61-67.) Furthermore, hybridization studies show stimulatory effects of both glucocorticosteroids and thyroid hormone on the glutamine synthetase mRNA level. Attempts to localize glutamine synthetase mRNA within the liver acinus by selective destruction of the pericentral zone failed because of generally low levels of liver mRNAs after CC! 4 poisoning. In contrast to the situation after birth, significantly higher glutamine synthetase mRNA/enzyme activity ratios in fetal rat liver point to the presence of additional post-transcriptional control mechanisms before birth. These findings complement similar observations on carbamoylphosphate synthetase gene expression (De Groot et al. (1986) Biochim. Biophys. Acta 866, 61-67).","publication_date":{"day":null,"month":null,"year":1987,"errors":{}},"publication_name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","grobid_abstract_attachment_id":68161938},"translated_abstract":null,"internal_url":"https://www.academia.edu/50021645/Reciprocal_regulation_of_glutamine_synthetase_and_carbamoylphosphate_synthetase_levels_in_rat_liver","translated_internal_url":"","created_at":"2021-07-17T10:14:13.864-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":68161938,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/68161938/thumbnails/1.jpg","file_name":"0167-4781_2887_2990103-520210717-3950-1c2wl7r.pdf","download_url":"https://www.academia.edu/attachments/68161938/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Reciprocal_regulation_of_glutamine_synth.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/68161938/0167-4781_2887_2990103-520210717-3950-1c2wl7r-libre.pdf?1626542304=\u0026response-content-disposition=attachment%3B+filename%3DReciprocal_regulation_of_glutamine_synth.pdf\u0026Expires=1732831242\u0026Signature=RUwNQEQuZqyyGAnJ1V0iSe4VE8199sTypHGaW4UeQAFGqOGgpyPy6h75L7tKIR9CJMZukNhx~XyyWLy1DfBoIEWxLmLwUJGQqLiVOUtISabbEPwhiCFnWIr~a6EZeDE8sa7OekAdtdQqZHWDBqylOOpH1T2ifZEe4X4i83VlNtGZAe8fM2K9zK7dkncys~X6UqYjoh8PMNzxWmCK9mBG8CnW5RvAcQB-k25yeScFSDZgRV4bSIIc-FJsW0huWC9yZUqrwSq7JsgqfFjm9T8Ea6F-tcV9StwXpg9kx6qdGpXcAiWupMaMgu7P8GR4ZXsGGLbEMcKnrmduCRZ-1BL1ug__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Reciprocal_regulation_of_glutamine_synthetase_and_carbamoylphosphate_synthetase_levels_in_rat_liver","translated_slug":"","page_count":10,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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Rabbit antiserum was raised against purified carbamoyl-phosphate synthase (ammonia) from rat l...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">1. Rabbit antiserum was raised against purified carbamoyl-phosphate synthase (ammonia) from rat liver. 2. The antiserum proved to be specific in double-diffusion test and reacted in an in situ immunohistochemical test on rat liver proteins fractionated on a sodium dodecyl sulphate polyacrylamide gel only in the region where carbamoyl-phosphate synthase (ammonia) migrated. 3. This antiserum was used for setting up a radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) in cetyltrimethylammonium bromide extracts of rat liver. To obtain reproducible results in this assay it was necessary to treat the unlabelled ligand with sodium dodecyl sulphate and dithiothreitol. This treatment led to a large increase in the percentage of labelled ligand displaceable by added unlabelled ligand. 4. Radioimmunochemical determination showed that adult rat liver (3-month old) contains 5.5 mg carbamoyl-phosphate synthase (ammonia) protein per gram wet weight.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021643"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021643"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021643; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50021643]").text(description); $(".js-view-count[data-work-id=50021643]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50021643; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50021643']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50021643, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=50021643]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50021643,"title":"Radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) content of adult rat liver","translated_title":"","metadata":{"abstract":"1. 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Radioimmunochemical determination showed that adult rat liver (3-month old) contains 5.5 mg carbamoyl-phosphate synthase (ammonia) protein per gram wet weight.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":1980,"errors":{}},"publication_name":"Biochimica et Biophysica Acta (BBA) - General Subjects"},"translated_abstract":"1. Rabbit antiserum was raised against purified carbamoyl-phosphate synthase (ammonia) from rat liver. 2. The antiserum proved to be specific in double-diffusion test and reacted in an in situ immunohistochemical test on rat liver proteins fractionated on a sodium dodecyl sulphate polyacrylamide gel only in the region where carbamoyl-phosphate synthase (ammonia) migrated. 3. This antiserum was used for setting up a radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) in cetyltrimethylammonium bromide extracts of rat liver. To obtain reproducible results in this assay it was necessary to treat the unlabelled ligand with sodium dodecyl sulphate and dithiothreitol. This treatment led to a large increase in the percentage of labelled ligand displaceable by added unlabelled ligand. 4. Radioimmunochemical determination showed that adult rat liver (3-month old) contains 5.5 mg carbamoyl-phosphate synthase (ammonia) protein per gram wet weight.","internal_url":"https://www.academia.edu/50021643/Radioimmunochemical_determination_of_carbamoyl_phosphate_synthase_ammonia_content_of_adult_rat_liver","translated_internal_url":"","created_at":"2021-07-17T10:14:13.690-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Radioimmunochemical_determination_of_carbamoyl_phosphate_synthase_ammonia_content_of_adult_rat_liver","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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The patterns of expression of three extracellular modulators of the transforming growth factor-beta superfamily of growth factors, Follistatin, Follistatin-like1, and Follistatin-like3, are described with respect to heart development. Follistatin is highly localized in the endocardium covering the developing cardiac valves. Follistatin-like1 is localized in the mesenchymal filling of the pharyngeal arches and broadly expressed in cells directly bordering myocardium. Follistatin-like3 is not expressed in the heart. Taken together, these observations are suggestive for a role for Follistatin in cardiac valvulogenesis and a role for Follistatin-like1 in controlling late heart muscle cell formation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021642"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021642"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021642; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50021642]").text(description); $(".js-view-count[data-work-id=50021642]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50021642; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50021642']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50021642, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=50021642]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50021642,"title":"Patterns of Expression of the Follistatin and Follistatin-Like1 Genes During Chicken Heart Development: A Potential Role in Valvulogenesis and Late Heart Muscle Cell Formation","translated_title":"","metadata":{"abstract":"The regulation of concentration and function of growth factors is of crucial importance to proper embryonic development of the heart. The patterns of expression of three extracellular modulators of the transforming growth factor-beta superfamily of growth factors, Follistatin, Follistatin-like1, and Follistatin-like3, are described with respect to heart development. Follistatin is highly localized in the endocardium covering the developing cardiac valves. Follistatin-like1 is localized in the mesenchymal filling of the pharyngeal arches and broadly expressed in cells directly bordering myocardium. Follistatin-like3 is not expressed in the heart. Taken together, these observations are suggestive for a role for Follistatin in cardiac valvulogenesis and a role for Follistatin-like1 in controlling late heart muscle cell formation.","publisher":"Wiley-Blackwell","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology"},"translated_abstract":"The regulation of concentration and function of growth factors is of crucial importance to proper embryonic development of the heart. The patterns of expression of three extracellular modulators of the transforming growth factor-beta superfamily of growth factors, Follistatin, Follistatin-like1, and Follistatin-like3, are described with respect to heart development. Follistatin is highly localized in the endocardium covering the developing cardiac valves. Follistatin-like1 is localized in the mesenchymal filling of the pharyngeal arches and broadly expressed in cells directly bordering myocardium. Follistatin-like3 is not expressed in the heart. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="47408714"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/47408714/Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System"><img alt="Research paper thumbnail of Identifying the Evolutionary Building Blocks of the Cardiac Conduction System" class="work-thumbnail" src="https://attachments.academia-assets.com/66515100/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/47408714/Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System">Identifying the Evolutionary Building Blocks of the Cardiac Conduction System</a></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a99b0acc42da9dfb0e9cd75dc5ac18dd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":66515100,"asset_id":47408714,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/66515100/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="47408714"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="47408714"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 47408714; 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These high heart rates are driven by very similar conduction systems consisting of an atrioventricular node that slows the electrical impulse and a His-Purkinje system that efficiently activates the ventricular chambers. While ectothermic vertebrates have similar contraction patterns, they do not possess anatomical evidence for a conduction system. This lack amongst extant ectotherms is surprising because mammals and birds evolved independently from reptilelike ancestors. Using conserved genetic markers, we found that the conduction system design of lizard (Anolis carolinensis and A. sagrei), frog (Xenopus laevis) and zebrafish (Danio rerio) adults is strikingly similar to that of embryos of mammals (mouse Mus musculus, and man) and chicken (Gallus gallus). Thus, in ectothermic adults, the slow conducting atrioventricular canal muscle is present, no fibrous insulating plane is formed, and the spongy ventricle serves the dual purpose of conduction and contraction. Optical mapping showed base-to-apex activation of the ventricles of the ectothermic animals, similar to the activation pattern of mammalian and avian embryonic ventricles and to the His-Purkinje systems of the formed hearts. Mammalian and avian ventricles uniquely develop thick compact walls and septum and, hence, form a discrete ventricular conduction system from the embryonic spongy ventricle. Our study uncovers the evolutionary building plan of heart and indicates that the building blocks of the conduction system of adult ectothermic vertebrates and embryos of endotherms are similar.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"PLoS ONE","grobid_abstract_attachment_id":66515100},"translated_abstract":null,"internal_url":"https://www.academia.edu/47408714/Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System","translated_internal_url":"","created_at":"2021-04-22T01:06:46.629-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":66515100,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/66515100/thumbnails/1.jpg","file_name":"00b7d51d47f1fd5bba000000.pdf","download_url":"https://www.academia.edu/attachments/66515100/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Identifying_the_Evolutionary_Building_Bl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/66515100/00b7d51d47f1fd5bba000000.pdf?1619078902=\u0026response-content-disposition=attachment%3B+filename%3DIdentifying_the_Evolutionary_Building_Bl.pdf\u0026Expires=1732831242\u0026Signature=FWhaKvdl4TD0tFC6OzKJeTZyff0MLWGRHB6SLl5KJiv9XtkDSIHUe5cGzdxEc-OOhl0BXibAnl7M5VwJJMWccGEk6EdoRlUvgaFqggtZaz9fhEWlGk2rL7uKIWcPjyXXv14v5rCli7kG6mKo9-vKO4z1K80ZclO2AKU8qk8DmqF08Hy0Coh~Y7-hBvru2SZ4OvD5eRDj~qUgJyNWJkxRL6ZlR2b7axwrMZCrA1FqBl1qVLTPCClLEgTXOU5qGM7UywSjdpuXxYlJu1yAzHRxFFq8BPumaalCXh7UVrdJHcVPj3p9-jhZdQ5F~RgRFz6HY~s3AC96D6Ao~LOJgXomug__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System","translated_slug":"","page_count":13,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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Moorman</a></span></div><div class="wp-workCard_item"><span>Circulation Research</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7f2b686d0b4f9c2b3fdc5845d338260b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54004524,"asset_id":34068769,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54004524/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34068769"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34068769"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34068769; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34068769]").text(description); $(".js-view-count[data-work-id=34068769]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34068769; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34068769']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34068769, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7f2b686d0b4f9c2b3fdc5845d338260b" } } $('.js-work-strip[data-work-id=34068769]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34068769,"title":"Pitx2c and Nkx2-5 Are Required for the Formation and Identity of the Pulmonary Myocardium","translated_title":"","metadata":{"grobid_abstract":"The pulmonary vein is sleeved by myocardium, which is a major source of atrial fibrillation and is involved in congenital sinus venosus defects. Little is known about the cellular origin and mechanism of formation of the pulmonary myocardium. We observed a biphasic process of pulmonary myocardium formation in mice. Firstly, a myocardial cell population forms de novo at the connection of the pulmonary vein and the atrium. Genetic labeling revealed that atrial cells do not contribute to this population, indicating it forms by differentiation of pulmonary mesenchymal cells. Secondly, these pulmonary myocardial cells initiate a phase of rapid proliferation and form the pulmonary myocardial sleeve. Pitx2c-deficient mice do not develop a pulmonary myocardial sleeve because they fail to form the initial pulmonary myocardial cells. Genetic-labeling analyses demonstrated that whereas the systemic venous return derives from Nkx2-5-negative precursors, the pulmonary myocardium derives from Nkx2-5-expressing precursors, indicating a distinct origin of the 2 venous systems. Nkx2-5 and its target gap-junction gene Cx40 are expressed in the atria and in the pulmonary myocardium but not in the systemic venous return, which expresses the essential pacemaker channel Hcn4. When Nkx2-5 protein level was lowered in a hypomorphic model, the pulmonary myocardium switched to a Cx40-negative, Hcn4-positive phenotype resembling that of the systemic venous return. In conclusion, our data suggest a cellular mechanism for pulmonary myocardium formation and highlight the key roles played by Pitx2c and Nkx2-5 in its formation and identity. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="24218601"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/24218601/Developmental_pattern_of_ANF_gene_expression_reveals_a_strict_localization_of_cardiac_chamber_formation_in_chicken"><img alt="Research paper thumbnail of Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken" class="work-thumbnail" src="https://attachments.academia-assets.com/44548975/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/24218601/Developmental_pattern_of_ANF_gene_expression_reveals_a_strict_localization_of_cardiac_chamber_formation_in_chicken">Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AMoorman1">A. Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://academicmedicalcentreuniversiteitvanamsterdam.academia.edu/MauriceVanDenHoff">Maurice Van Den Hoff</a></span></div><div class="wp-workCard_item"><span>The Anatomical record</span><span>, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber fo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. At HH9 cANF expression was fir...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ff105af027464c9633b9b8a49b04261f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44548975,"asset_id":24218601,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44548975/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218601"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218601"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218601; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24218601]").text(description); $(".js-view-count[data-work-id=24218601]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24218601; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24218601']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 24218601, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ff105af027464c9633b9b8a49b04261f" } } $('.js-work-strip[data-work-id=24218601]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24218601,"title":"Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken","translated_title":"","metadata":{"abstract":"In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. 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Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://academicmedicalcentreuniversiteitvanamsterdam.academia.edu/MauriceVanDenHoff">Maurice Van Den Hoff</a></span></div><div class="wp-workCard_item"><span>The Anatomical Record</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0045561ecd970ac10f9c2a3874fe6fd4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44548974,"asset_id":24218600,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44548974/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218600"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218600"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218600; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24218600]").text(description); $(".js-view-count[data-work-id=24218600]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24218600; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24218600']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 24218600, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0045561ecd970ac10f9c2a3874fe6fd4" } } $('.js-work-strip[data-work-id=24218600]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24218600,"title":"Expression of cVg1 mRNA during chicken embryonic development","translated_title":"","metadata":{"grobid_abstract":"Using degenerated PCR-primers to identify known and novel BMPs that are expressed in the developing chicken heart, we identified not only BMP2, -4, and -7 mRNA, but also the TGF␤ superfamily member cVg1. 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Anat Rec Part A 273A:603-608, 2003.","publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"The Anatomical Record","grobid_abstract_attachment_id":44548974},"translated_abstract":null,"internal_url":"https://www.academia.edu/24218600/Expression_of_cVg1_mRNA_during_chicken_embryonic_development","translated_internal_url":"","created_at":"2016-04-08T13:28:27.689-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18709398,"work_id":24218600,"tagging_user_id":46673754,"tagged_user_id":46881112,"co_author_invite_id":4263284,"email":"m***f@amc.nl","affiliation":"Academic Medical Centre/ Universiteit van Amsterdam","display_order":0,"name":"Maurice Van Den Hoff","title":"Expression of cVg1 mRNA during chicken embryonic development"}],"downloadable_attachments":[{"id":44548974,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44548974/thumbnails/1.jpg","file_name":"Expression_of_cVg1_mRNA_during_chicken_e20160408-1443-1q8euqc.pdf","download_url":"https://www.academia.edu/attachments/44548974/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Expression_of_cVg1_mRNA_during_chicken_e.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44548974/Expression_of_cVg1_mRNA_during_chicken_e20160408-1443-1q8euqc-libre.pdf?1460147538=\u0026response-content-disposition=attachment%3B+filename%3DExpression_of_cVg1_mRNA_during_chicken_e.pdf\u0026Expires=1732831242\u0026Signature=Gsgvb-WV82aJMUSo4GaHMAZwJ~eHCda-iRmeKiOl~mXxtSnCwoBUgfZ41UsU~LGnrs-JyOBjmyS1OqhKlMD-j5yK8jSXR3Rf2dQird2QO6xs~tfkbiQMVklt4EuE4BThsKPes-6-qTIQxxkrA1j~cUaITU~~POhrvzwL4wA0NGY9f4sp8d6xMkPx-EAfirr5wl7xwJqZ8jpWUlgGs9URSA4GF4IRHBMm6fRliyX5FdwSUgR2XZ1rNvJNWt~wuwVPRvzZtsJYO7BOhJ8O5bbswjIZlXK2Du5C9X~RG5W32si6QlTCtkx2pbWWRPOxQsnVLvcgrcSMgWf6Jv39q~-f3g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Expression_of_cVg1_mRNA_during_chicken_embryonic_development","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://academicmedicalcentreuniversiteitvanamsterdam.academia.edu/MauriceVanDenHoff">Maurice Van Den Hoff</a></span></div><div class="wp-workCard_item"><span>Journal of Histochemistry and Cytochemistry</span><span>, 2006</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="895f76ac001c7fb13091cbcb08de23bd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44548973,"asset_id":24218599,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44548973/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218599"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218599"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218599; 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Irx1 and Irx2 were found to be expressed specifically in the ventricular septum from the onset of its formation onward. In fetal stages, the expression of both genes appeared to gradually become confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Irx3 was found to be expressed specifically in the trabeculated myocardium of the ventricles. Irx4 expression was observed in a segment of the linear heart tube and the atrioventricular canal and ventricular myocardium including the inner curvature after looping, resembling the pattern of MLC2V. Transcripts for Irx5 were detected specifically in the endocardium lining the ventricular and atrial working myocardium that also expressed von Willebrand factor, but were absent from the endocardium of the endocardial cushions, i.e., the atrioventricular canal, inner curvature, and outflow tract. The spatiodevelopmental pattern of Irx5 matched that of ANF, a marker for the forming working myocardium of the chambers. Taken together, all members of the Irx gene family were found to be expressed in highly specific patterns in the developing mouse heart, suggesting a critical role in the specification of the distinct components of the four-chambered heart.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218598"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218598"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218598; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24218598]").text(description); $(".js-view-count[data-work-id=24218598]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24218598; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24218598']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 24218598, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=24218598]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24218598,"title":"Patterning the Embryonic Heart: Identification of Five Mouse Iroquois Homeobox Genes in the Developing Heart","translated_title":"","metadata":{"abstract":"We isolated cDNAs of mouse Iroquois-related homeobox genes Irx1, -2, -3, -4, and -5 and characterized their patterns of expression in the developing heart. Irx1 and Irx2 were found to be expressed specifically in the ventricular septum from the onset of its formation onward. In fetal stages, the expression of both genes appeared to gradually become confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Irx3 was found to be expressed specifically in the trabeculated myocardium of the ventricles. Irx4 expression was observed in a segment of the linear heart tube and the atrioventricular canal and ventricular myocardium including the inner curvature after looping, resembling the pattern of MLC2V. Transcripts for Irx5 were detected specifically in the endocardium lining the ventricular and atrial working myocardium that also expressed von Willebrand factor, but were absent from the endocardium of the endocardial cushions, i.e., the atrioventricular canal, inner curvature, and outflow tract. The spatiodevelopmental pattern of Irx5 matched that of ANF, a marker for the forming working myocardium of the chambers. Taken together, all members of the Irx gene family were found to be expressed in highly specific patterns in the developing mouse heart, suggesting a critical role in the specification of the distinct components of the four-chambered heart.","publication_date":{"day":null,"month":null,"year":2000,"errors":{}},"publication_name":"Developmental Biology"},"translated_abstract":"We isolated cDNAs of mouse Iroquois-related homeobox genes Irx1, -2, -3, -4, and -5 and characterized their patterns of expression in the developing heart. Irx1 and Irx2 were found to be expressed specifically in the ventricular septum from the onset of its formation onward. In fetal stages, the expression of both genes appeared to gradually become confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Irx3 was found to be expressed specifically in the trabeculated myocardium of the ventricles. Irx4 expression was observed in a segment of the linear heart tube and the atrioventricular canal and ventricular myocardium including the inner curvature after looping, resembling the pattern of MLC2V. Transcripts for Irx5 were detected specifically in the endocardium lining the ventricular and atrial working myocardium that also expressed von Willebrand factor, but were absent from the endocardium of the endocardial cushions, i.e., the atrioventricular canal, inner curvature, and outflow tract. The spatiodevelopmental pattern of Irx5 matched that of ANF, a marker for the forming working myocardium of the chambers. Taken together, all members of the Irx gene family were found to be expressed in highly specific patterns in the developing mouse heart, suggesting a critical role in the specification of the distinct components of the four-chambered heart.","internal_url":"https://www.academia.edu/24218598/Patterning_the_Embryonic_Heart_Identification_of_Five_Mouse_Iroquois_Homeobox_Genes_in_the_Developing_Heart","translated_internal_url":"","created_at":"2016-04-08T13:28:27.278-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18709401,"work_id":24218598,"tagging_user_id":46673754,"tagged_user_id":null,"co_author_invite_id":4263285,"email":"a***n@amc.nl","display_order":0,"name":"Anton Morman","title":"Patterning the Embryonic Heart: Identification of Five Mouse Iroquois Homeobox Genes in the Developing Heart"}],"downloadable_attachments":[],"slug":"Patterning_the_Embryonic_Heart_Identification_of_Five_Mouse_Iroquois_Homeobox_Genes_in_the_Developing_Heart","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. Moorman","url":"https://independent.academia.edu/AMoorman1"},"attachments":[],"research_interests":[{"id":1083,"name":"Developmental Biology","url":"https://www.academia.edu/Documents/in/Developmental_Biology"},{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":80043,"name":"von Willebrand factor","url":"https://www.academia.edu/Documents/in/von_Willebrand_factor"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131495,"name":"Heart","url":"https://www.academia.edu/Documents/in/Heart"},{"id":229238,"name":"Developmental","url":"https://www.academia.edu/Documents/in/Developmental"},{"id":370195,"name":"Body Patterning","url":"https://www.academia.edu/Documents/in/Body_Patterning"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":999803,"name":"Gene Family","url":"https://www.academia.edu/Documents/in/Gene_Family"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="16407867"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/16407867/Developmental_pattern_of_ANF_gene_expression_reveals_a_strict_localization_of_cardiac_chamber_formation_in_chicken"><img alt="Research paper thumbnail of Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken" class="work-thumbnail" src="https://attachments.academia-assets.com/42495653/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/16407867/Developmental_pattern_of_ANF_gene_expression_reveals_a_strict_localization_of_cardiac_chamber_formation_in_chicken">Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AMoorman1">A. Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ArjanHouweling">Arjan Houweling</a></span></div><div class="wp-workCard_item"><span>The Anatomical record</span><span>, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber fo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. At HH9 cANF expression was fir...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b1d944f0107e758961898e99c22ddd47" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42495653,"asset_id":16407867,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42495653/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="16407867"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="16407867"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16407867; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16407867]").text(description); $(".js-view-count[data-work-id=16407867]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16407867; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='16407867']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 16407867, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b1d944f0107e758961898e99c22ddd47" } } $('.js-work-strip[data-work-id=16407867]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":16407867,"title":"Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken","translated_title":"","metadata":{"abstract":"In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. At HH9 cANF expression was fir...","publication_date":{"day":null,"month":null,"year":2002,"errors":{}},"publication_name":"The Anatomical record"},"translated_abstract":"In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. At HH9 cANF expression was fir...","internal_url":"https://www.academia.edu/16407867/Developmental_pattern_of_ANF_gene_expression_reveals_a_strict_localization_of_cardiac_chamber_formation_in_chicken","translated_internal_url":"","created_at":"2015-10-02T10:39:22.541-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":35550583,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":6591340,"work_id":16407867,"tagging_user_id":35550583,"tagged_user_id":46673754,"co_author_invite_id":1468379,"email":"a***n@amc.uva.nl","display_order":0,"name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="16407858"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/16407858/Expression_of_cVg1_mRNA_during_chicken_embryonic_development"><img alt="Research paper thumbnail of Expression of cVg1 mRNA during chicken embryonic development" class="work-thumbnail" src="https://attachments.academia-assets.com/42495646/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/16407858/Expression_of_cVg1_mRNA_during_chicken_embryonic_development">Expression of cVg1 mRNA during chicken embryonic development</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AMoorman1">A. Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ArjanHouweling">Arjan Houweling</a></span></div><div class="wp-workCard_item"><span>The Anatomical Record</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fa99aece7ac686f5a10a50bb690c055f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42495646,"asset_id":16407858,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42495646/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="16407858"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="16407858"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16407858; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16407858]").text(description); $(".js-view-count[data-work-id=16407858]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16407858; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='16407858']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 16407858, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fa99aece7ac686f5a10a50bb690c055f" } } $('.js-work-strip[data-work-id=16407858]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":16407858,"title":"Expression of cVg1 mRNA during chicken embryonic development","translated_title":"","metadata":{"grobid_abstract":"Using degenerated PCR-primers to identify known and novel BMPs that are expressed in the developing chicken heart, we identified not only BMP2, -4, and -7 mRNA, but also the TGF␤ superfamily member cVg1. The expression pattern of cVg1 mRNA was determined during chicken development from HH4 to HH44. In early developmental stages, cVg1 mRNA is expressed in the primitive streak, paraxial mesoderm, developing somites, and developing neural tube. Subsequently, cVg1 mRNA is expressed in the developing central and peripheral nervous system, retina, auditory vesicle, notochord, lung alveoli, and olfactory mucosa. In the heart, cVg1 is initially expressed through the linear heart tube, but becomes restricted to the forming chamber myocardium, in an expression domain similar to that of atrial natriuretic factor (ANF) mRNA. Anat Rec Part A 273A:603-608, 2003.","publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"The Anatomical Record","grobid_abstract_attachment_id":42495646},"translated_abstract":null,"internal_url":"https://www.academia.edu/16407858/Expression_of_cVg1_mRNA_during_chicken_embryonic_development","translated_internal_url":"","created_at":"2015-10-02T10:39:20.793-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":35550583,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":6591347,"work_id":16407858,"tagging_user_id":35550583,"tagged_user_id":46673754,"co_author_invite_id":1468379,"email":"a***n@amc.uva.nl","display_order":0,"name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="5004082" id="papers"><div class="js-work-strip profile--work_container" data-work-id="111793320"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/111793320/The_organization_of_the_histone_genes_in_the_genome_of_Xenopus_laevis"><img alt="Research paper thumbnail of The organization of the histone genes in the genome of Xenopus laevis" class="work-thumbnail" src="https://attachments.academia-assets.com/110043769/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/111793320/The_organization_of_the_histone_genes_in_the_genome_of_Xenopus_laevis">The organization of the histone genes in the genome of Xenopus laevis</a></div><div class="wp-workCard_item"><span>Nucleic Acids Research</span><span>, 1981</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bdc8d49ac6979bcc9ef2515129613eff" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":110043769,"asset_id":111793320,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/110043769/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="111793320"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="111793320"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 111793320; 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For that purpose, Southern blots of genomic DNA, that was digested with several restriction enzymes, were hybridized with radioactively labeled DNA fragments from clone Xl-hi-1 (14), containing genes for Xenopus histones H2A, H2B, H3 and H4. In the DNA of all animals that were screened we found a major repeating unit of 14 kilobasepairs , which contains genes for histones H2A, H2B, H3 and H4 (H1 not tested) and is represented up to 30 times in the genome. The orde of the genes in this major repeating unit is: H4-H3-H2A-H2B. This order is different from that in the histone DNA of clone Xl-hi-1, i.e. H3-H4-H2A-H2B. In addition to the genes in the major repeating unit, histone genes are present in unique restriction fragments in numbers that vary from one animal to another. The restriction patterns for the histone genes in these unique fragments were found to be different for all eight Xenopus individuals that were screened. The cloned Xenopus histone gene fragment Xl-hi-1 represents such a unique fragment and is not present in the DNA of each single individual. The total number of genes coding for each of the nucleosomal histones is 45-50 per haploid genome.","publication_date":{"day":null,"month":null,"year":1981,"errors":{}},"publication_name":"Nucleic Acids Research","grobid_abstract_attachment_id":110043769},"translated_abstract":null,"internal_url":"https://www.academia.edu/111793320/The_organization_of_the_histone_genes_in_the_genome_of_Xenopus_laevis","translated_internal_url":"","created_at":"2023-12-18T23:15:25.778-08:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":110043769,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/110043769/thumbnails/1.jpg","file_name":"e328e1b3f0c3a40cc60a906cc114e3dcc909.pdf","download_url":"https://www.academia.edu/attachments/110043769/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"The_organization_of_the_histone_genes_in.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/110043769/e328e1b3f0c3a40cc60a906cc114e3dcc909-libre.pdf?1704404449=\u0026response-content-disposition=attachment%3B+filename%3DThe_organization_of_the_histone_genes_in.pdf\u0026Expires=1732831241\u0026Signature=bk5100CqII3704SORMt7iRRUvfdvICYoIl4--HKpG6cjOxrI0jjs9RpiaWxubvVgK0rv87kXx9GukLeNRTztUwGlO7mbnCXGahmo8niklf5JOPqeZ9CAkRMlT46InELWrwECLgtoV43nXKIir6Ihw~7RK6ezouZfnjJRpdxyrDv7rNBy942Vi3uXLxNoUpfW~SznELF3gzg07HQxlTKM-4rHjyHi-JkKACpq-6qgUP62v56-fIbyJBtedlsKA80PyzDDVGQqzcANxIVh-leqdR4erVolbdjrvBP8ZjkWDUmOEwhJv07N1szEoxugQCCk49oqmpSA1JghZxf2A03mSw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"The_organization_of_the_histone_genes_in_the_genome_of_Xenopus_laevis","translated_slug":"","page_count":16,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="107727563"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/107727563/Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium"><img alt="Research paper thumbnail of Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium" class="work-thumbnail" src="https://attachments.academia-assets.com/106312535/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/107727563/Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium">Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium</a></div><div class="wp-workCard_item"><span>Journal of Molecular and Cellular Cardiology</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6f9a6d7e0d64e0dcb886f24dc3ba7511" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":106312535,"asset_id":107727563,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/106312535/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="107727563"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="107727563"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 107727563; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=107727563]").text(description); $(".js-view-count[data-work-id=107727563]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 107727563; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='107727563']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 107727563, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6f9a6d7e0d64e0dcb886f24dc3ba7511" } } $('.js-work-strip[data-work-id=107727563]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":107727563,"title":"Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium","translated_title":"","metadata":{"publisher":"Elsevier BV","grobid_abstract":"Mouse embryonic stem cells easily differentiate towards the cardiac lineage making them suitable as an in vitro model to study cardiogenesis and as a potential source of transplantable cells. In this study we show by in situ hybridisation that about 30% of the volume of cultures of differentiating embryonic stem cells consists of cardiomyocytes. RT-PCR analyses showed that the transcription factors Nkx2.5, Gata4, MeJ2c and Irx4 were expressed at levels in the same order of magnitude as the levels observed in embryonic, neonatal and adult hearts. Atrial natriuretic factor and Connexin40, associated with chamber formation in vivo, are expressed at relatively low levels, similar to those observed at early heart development in vivo. To facilitate the isolation of embryonic stem cell-derived cardiomyocytes, a cell line was constructed by stable transfection of the aminoglycoside phosphotransferase cDNA driven by the cardiac-specific distant upstream part of the Na + /Ca 2+ exchanger promoter. To accomplish single copy integration, the construct was inserted into the hypoxanthine phosphoribosyltransferase locus of HM1 embryonic stem cells by homologous recombination. Cardiac-specific resistance to G418sulphate (neomycin) allowed isolation of a pure population of cardiomyocytes. Genetically selected and unselected cell populations were characterised electrophysiologically using patch clamp. To explore whether clusters of cells have a similar differentiation profile, action potentials were measured in aggregates of differentiating embryonic stem cells, using a new method based on the voltage dependent fluorescent dye di-4-ANEPPS. Both whole-cell recordings using patch clamp and optical measurements with di-4-ANEPPS of the action potential showed that upstroke velocity increases and action potential duration decreases with differentiation time, accompanied by a decrease in action potential interval, suggesting the initiation of the developmental program underlying the formation of chamber myocardium.","publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"Journal of Molecular and Cellular Cardiology","grobid_abstract_attachment_id":106312535},"translated_abstract":null,"internal_url":"https://www.academia.edu/107727563/Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium","translated_internal_url":"","created_at":"2023-10-06T23:53:44.266-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":106312535,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/106312535/thumbnails/1.jpg","file_name":"31454_UBA002000996_11.pdf","download_url":"https://www.academia.edu/attachments/106312535/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0MSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Cardiomyocytes_purified_from_differentia.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/106312535/31454_UBA002000996_11-libre.pdf?1696664297=\u0026response-content-disposition=attachment%3B+filename%3DCardiomyocytes_purified_from_differentia.pdf\u0026Expires=1732831241\u0026Signature=EYJmfEJV94Y53-MfpzydvEheUbCncpVGIlQe5CBREhAEIABChvt2Dqu-Ns~AivUzCcrkGnFdxgrCik5buFGZ5z0n3JZhPDqng-d4A0HK~2b6lS6Z~c9V1qX0C7zW59c1zHZQqNiZEa9Deq-k5ulw0qUbLKTwBGEDVJdISuf6T~b-Fhf6xpEUecaNkvemeNGPa8yXiQCxkHHoo8vRHt-v6i~9XZtzpvbG4cfwA-lx7rVUPqNidSVTqbog5FkOhaGqdV7AZZDX0rKLp-mNO-uNrSF-ZC5gjmGOoo4Q23sB7Vvd9Fyw7UamMCsXEF~pEOlFMypm5zx4p530Fg8ZmuX~Aw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Cardiomyocytes_purified_from_differentiated_embryonic_stem_cells_exhibit_characteristics_of_early_chamber_myocardium","translated_slug":"","page_count":25,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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Enzyme activity, mRNA, and gene transcription of cholesterol 7c-hydroxylase were predominant in pericentral hepatocytes of control rats, being 7.9-, 9.9-, and 4.4-fold higher than in periportal hepatocytes, respectively. Similar localization was found for sterol 27-hydroxylase: 2.9-, 2.5-, and 1.7-fold higher enzyme activity, mRNA, and gene transcription, respectively, was found in pericentral hepatocytes. Interruption of the enterohepatic circulation with colestid resulted in upregulation of these parameters for both enzymes, as a consequence of stimulated gene expression mainly in the periportal zone. In contrast, mRNA levels and gene transcription of 3-hydroxy-3-methylglutaryl CoA reductase showed opposite lobular distribution. Selective periportal expression for the latter was enhanced, but remained local, after colestid treatment. In situ hybridization showed unambiguously that cholesterol 7a-hydroxylase mRNA is localized exclusively in the pericentral zone and that sterol 27-hydroxylase mRNA is expressed preferentially in the pericentral region, though less pronounced. Administration of colestid led to expression of both genes within a larger area of the liver lobulus. In conclusion, we suggest that cholesterol 7a-hydroxylase and sterol 27-hydroxylase are coordinately regulated by the bile acid gradient over the lobulus, resulting in predominant expression in the pericentral zone. 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Potential repair by cell grafting or mobilizing endogenous cells is of particular interest for possible treatments for heart disease, where the poor capacity for cardiomyocyte proliferation probably contributes to the irreversibility of heart failure. Knowledge of the molecular mechanisms that underly formation of heart muscle cells might provide opportunities to repair the diseased heart by induction of (trans) differentiation of endogenous or exogenous cells into heart muscle cells. We briefly review the molecular mechanisms involved in early development of the linear heart tube by differentiation of mesodermal cells into heart muscle cells. Because the initial heart tube does not comprise all the cardiac compartments present in the adult heart, heart muscle cells are added to the distal borders of the tube and within the tube. At both distal borders, mesodermal cell are recruited into the cardiac lineage and, within the heart tube, muscular septa are formed. In this review, the relative late additions of heart muscle cells to the linear heart tube are described and the potential underlying molecular mechanisms are discussed.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021647"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021647"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021647; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50021647]").text(description); $(".js-view-count[data-work-id=50021647]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50021647; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50021647']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50021647, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=50021647]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50021647,"title":"Making more heart muscle","translated_title":"","metadata":{"abstract":"Postnatally, heart muscle cells almost completely lose their ability to divide, which makes their loss after trauma irreversible. Potential repair by cell grafting or mobilizing endogenous cells is of particular interest for possible treatments for heart disease, where the poor capacity for cardiomyocyte proliferation probably contributes to the irreversibility of heart failure. Knowledge of the molecular mechanisms that underly formation of heart muscle cells might provide opportunities to repair the diseased heart by induction of (trans) differentiation of endogenous or exogenous cells into heart muscle cells. We briefly review the molecular mechanisms involved in early development of the linear heart tube by differentiation of mesodermal cells into heart muscle cells. Because the initial heart tube does not comprise all the cardiac compartments present in the adult heart, heart muscle cells are added to the distal borders of the tube and within the tube. At both distal borders, mesodermal cell are recruited into the cardiac lineage and, within the heart tube, muscular septa are formed. In this review, the relative late additions of heart muscle cells to the linear heart tube are described and the potential underlying molecular mechanisms are discussed.","publisher":"Wiley-Blackwell","publication_date":{"day":null,"month":null,"year":2004,"errors":{}},"publication_name":"BioEssays"},"translated_abstract":"Postnatally, heart muscle cells almost completely lose their ability to divide, which makes their loss after trauma irreversible. Potential repair by cell grafting or mobilizing endogenous cells is of particular interest for possible treatments for heart disease, where the poor capacity for cardiomyocyte proliferation probably contributes to the irreversibility of heart failure. Knowledge of the molecular mechanisms that underly formation of heart muscle cells might provide opportunities to repair the diseased heart by induction of (trans) differentiation of endogenous or exogenous cells into heart muscle cells. We briefly review the molecular mechanisms involved in early development of the linear heart tube by differentiation of mesodermal cells into heart muscle cells. Because the initial heart tube does not comprise all the cardiac compartments present in the adult heart, heart muscle cells are added to the distal borders of the tube and within the tube. At both distal borders, mesodermal cell are recruited into the cardiac lineage and, within the heart tube, muscular septa are formed. In this review, the relative late additions of heart muscle cells to the linear heart tube are described and the potential underlying molecular mechanisms are discussed.","internal_url":"https://www.academia.edu/50021647/Making_more_heart_muscle","translated_internal_url":"","created_at":"2021-07-17T10:14:14.018-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Making_more_heart_muscle","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="50021646"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/50021646/Developmental_and_hormonal_regulation_of_carbamoyl_phosphate_synthase_gene_expression_in_rat_liver_Evidence_for_control_mechanisms_at_different_levels_in_the_perinatal_period"><img alt="Research paper thumbnail of Developmental and hormonal regulation of carbamoyl-phosphate synthase gene expression in rat liver: Evidence for control mechanisms at different levels in the perinatal period" class="work-thumbnail" src="https://attachments.academia-assets.com/68161936/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/50021646/Developmental_and_hormonal_regulation_of_carbamoyl_phosphate_synthase_gene_expression_in_rat_liver_Evidence_for_control_mechanisms_at_different_levels_in_the_perinatal_period">Developmental and hormonal regulation of carbamoyl-phosphate synthase gene expression in rat liver: Evidence for control mechanisms at different levels in the perinatal period</a></div><div class="wp-workCard_item"><span>Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression</span><span>, 1986</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1d6019f14d391c143f190d1a794bd47b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":68161936,"asset_id":50021646,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/68161936/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021646"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021646"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021646; 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In addition, immunohistochemistry shows that under these conditions the distribution of carbamoylphosphate synthetase is expanded over the entire liver acinus, whereas that of glutamine synthetase is reduced to very few cells bordering the central (terminal hepatic) veins. Using a newly isolated cDNA complementary to rat liver glutamine synthetase mRNA, we show that this regulation is primarily effected at a pretranslational level. (For data on carbamoylphosphate synthetase mRNA levels, see De Groot et al. (1986) Biochim. Biophys. Acta 866, 61-67.) Furthermore, hybridization studies show stimulatory effects of both glucocorticosteroids and thyroid hormone on the glutamine synthetase mRNA level. Attempts to localize glutamine synthetase mRNA within the liver acinus by selective destruction of the pericentral zone failed because of generally low levels of liver mRNAs after CC! 4 poisoning. In contrast to the situation after birth, significantly higher glutamine synthetase mRNA/enzyme activity ratios in fetal rat liver point to the presence of additional post-transcriptional control mechanisms before birth. These findings complement similar observations on carbamoylphosphate synthetase gene expression (De Groot et al. (1986) Biochim. Biophys. Acta 866, 61-67).","publication_date":{"day":null,"month":null,"year":1987,"errors":{}},"publication_name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","grobid_abstract_attachment_id":68161938},"translated_abstract":null,"internal_url":"https://www.academia.edu/50021645/Reciprocal_regulation_of_glutamine_synthetase_and_carbamoylphosphate_synthetase_levels_in_rat_liver","translated_internal_url":"","created_at":"2021-07-17T10:14:13.864-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":68161938,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/68161938/thumbnails/1.jpg","file_name":"0167-4781_2887_2990103-520210717-3950-1c2wl7r.pdf","download_url":"https://www.academia.edu/attachments/68161938/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Reciprocal_regulation_of_glutamine_synth.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/68161938/0167-4781_2887_2990103-520210717-3950-1c2wl7r-libre.pdf?1626542304=\u0026response-content-disposition=attachment%3B+filename%3DReciprocal_regulation_of_glutamine_synth.pdf\u0026Expires=1732831242\u0026Signature=RUwNQEQuZqyyGAnJ1V0iSe4VE8199sTypHGaW4UeQAFGqOGgpyPy6h75L7tKIR9CJMZukNhx~XyyWLy1DfBoIEWxLmLwUJGQqLiVOUtISabbEPwhiCFnWIr~a6EZeDE8sa7OekAdtdQqZHWDBqylOOpH1T2ifZEe4X4i83VlNtGZAe8fM2K9zK7dkncys~X6UqYjoh8PMNzxWmCK9mBG8CnW5RvAcQB-k25yeScFSDZgRV4bSIIc-FJsW0huWC9yZUqrwSq7JsgqfFjm9T8Ea6F-tcV9StwXpg9kx6qdGpXcAiWupMaMgu7P8GR4ZXsGGLbEMcKnrmduCRZ-1BL1ug__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Reciprocal_regulation_of_glutamine_synthetase_and_carbamoylphosphate_synthetase_levels_in_rat_liver","translated_slug":"","page_count":10,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="50021644"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/50021644/Regulation_of_mRNA_levels_of_rat_liver_carbamoylphosphate_synthetase_by_glucocorticosteroids_and_cyclic_AMP_as_estimated_with_a_specific_cDNA"><img alt="Research paper thumbnail of Regulation of mRNA levels of rat liver carbamoylphosphate synthetase by glucocorticosteroids and cyclic AMP as estimated with a specific cDNA" class="work-thumbnail" src="https://attachments.academia-assets.com/68161939/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/50021644/Regulation_of_mRNA_levels_of_rat_liver_carbamoylphosphate_synthetase_by_glucocorticosteroids_and_cyclic_AMP_as_estimated_with_a_specific_cDNA">Regulation of mRNA levels of rat liver carbamoylphosphate synthetase by glucocorticosteroids and cyclic AMP as estimated with a specific cDNA</a></div><div class="wp-workCard_item"><span>Biochemical and Biophysical Research Communications</span><span>, 1984</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6225d6711433618739abe7bea3007204" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":68161939,"asset_id":50021644,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/68161939/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021644"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021644"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021644; 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Rabbit antiserum was raised against purified carbamoyl-phosphate synthase (ammonia) from rat l...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">1. Rabbit antiserum was raised against purified carbamoyl-phosphate synthase (ammonia) from rat liver. 2. The antiserum proved to be specific in double-diffusion test and reacted in an in situ immunohistochemical test on rat liver proteins fractionated on a sodium dodecyl sulphate polyacrylamide gel only in the region where carbamoyl-phosphate synthase (ammonia) migrated. 3. This antiserum was used for setting up a radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) in cetyltrimethylammonium bromide extracts of rat liver. To obtain reproducible results in this assay it was necessary to treat the unlabelled ligand with sodium dodecyl sulphate and dithiothreitol. This treatment led to a large increase in the percentage of labelled ligand displaceable by added unlabelled ligand. 4. Radioimmunochemical determination showed that adult rat liver (3-month old) contains 5.5 mg carbamoyl-phosphate synthase (ammonia) protein per gram wet weight.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021643"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021643"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021643; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50021643]").text(description); $(".js-view-count[data-work-id=50021643]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50021643; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50021643']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50021643, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=50021643]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50021643,"title":"Radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) content of adult rat liver","translated_title":"","metadata":{"abstract":"1. Rabbit antiserum was raised against purified carbamoyl-phosphate synthase (ammonia) from rat liver. 2. The antiserum proved to be specific in double-diffusion test and reacted in an in situ immunohistochemical test on rat liver proteins fractionated on a sodium dodecyl sulphate polyacrylamide gel only in the region where carbamoyl-phosphate synthase (ammonia) migrated. 3. This antiserum was used for setting up a radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) in cetyltrimethylammonium bromide extracts of rat liver. To obtain reproducible results in this assay it was necessary to treat the unlabelled ligand with sodium dodecyl sulphate and dithiothreitol. This treatment led to a large increase in the percentage of labelled ligand displaceable by added unlabelled ligand. 4. Radioimmunochemical determination showed that adult rat liver (3-month old) contains 5.5 mg carbamoyl-phosphate synthase (ammonia) protein per gram wet weight.","publisher":"Elsevier BV","publication_date":{"day":null,"month":null,"year":1980,"errors":{}},"publication_name":"Biochimica et Biophysica Acta (BBA) - General Subjects"},"translated_abstract":"1. Rabbit antiserum was raised against purified carbamoyl-phosphate synthase (ammonia) from rat liver. 2. The antiserum proved to be specific in double-diffusion test and reacted in an in situ immunohistochemical test on rat liver proteins fractionated on a sodium dodecyl sulphate polyacrylamide gel only in the region where carbamoyl-phosphate synthase (ammonia) migrated. 3. This antiserum was used for setting up a radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) in cetyltrimethylammonium bromide extracts of rat liver. To obtain reproducible results in this assay it was necessary to treat the unlabelled ligand with sodium dodecyl sulphate and dithiothreitol. This treatment led to a large increase in the percentage of labelled ligand displaceable by added unlabelled ligand. 4. Radioimmunochemical determination showed that adult rat liver (3-month old) contains 5.5 mg carbamoyl-phosphate synthase (ammonia) protein per gram wet weight.","internal_url":"https://www.academia.edu/50021643/Radioimmunochemical_determination_of_carbamoyl_phosphate_synthase_ammonia_content_of_adult_rat_liver","translated_internal_url":"","created_at":"2021-07-17T10:14:13.690-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Radioimmunochemical_determination_of_carbamoyl_phosphate_synthase_ammonia_content_of_adult_rat_liver","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. Moorman","url":"https://independent.academia.edu/AMoorman1"},"attachments":[],"research_interests":[{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver"},{"id":118582,"name":"Physical sciences","url":"https://www.academia.edu/Documents/in/Physical_sciences"},{"id":193464,"name":"Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Sodium_Dodecyl_Sulfate-Polyacrylamide_Gel_Electrophoresis"},{"id":375054,"name":"Rats","url":"https://www.academia.edu/Documents/in/Rats"},{"id":1159038,"name":"Epitopes","url":"https://www.academia.edu/Documents/in/Epitopes"},{"id":1648955,"name":"Radioimmunoassay","url":"https://www.academia.edu/Documents/in/Radioimmunoassay"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"},{"id":2622517,"name":"Sodium Dodecyl Sulfate","url":"https://www.academia.edu/Documents/in/Sodium_Dodecyl_Sulfate"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="50021642"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/50021642/Patterns_of_Expression_of_the_Follistatin_and_Follistatin_Like1_Genes_During_Chicken_Heart_Development_A_Potential_Role_in_Valvulogenesis_and_Late_Heart_Muscle_Cell_Formation"><img alt="Research paper thumbnail of Patterns of Expression of the Follistatin and Follistatin-Like1 Genes During Chicken Heart Development: A Potential Role in Valvulogenesis and Late Heart Muscle Cell Formation" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/50021642/Patterns_of_Expression_of_the_Follistatin_and_Follistatin_Like1_Genes_During_Chicken_Heart_Development_A_Potential_Role_in_Valvulogenesis_and_Late_Heart_Muscle_Cell_Formation">Patterns of Expression of the Follistatin and Follistatin-Like1 Genes During Chicken Heart Development: A Potential Role in Valvulogenesis and Late Heart Muscle Cell Formation</a></div><div class="wp-workCard_item"><span>The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The regulation of concentration and function of growth factors is of crucial importance to proper...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The regulation of concentration and function of growth factors is of crucial importance to proper embryonic development of the heart. The patterns of expression of three extracellular modulators of the transforming growth factor-beta superfamily of growth factors, Follistatin, Follistatin-like1, and Follistatin-like3, are described with respect to heart development. Follistatin is highly localized in the endocardium covering the developing cardiac valves. Follistatin-like1 is localized in the mesenchymal filling of the pharyngeal arches and broadly expressed in cells directly bordering myocardium. Follistatin-like3 is not expressed in the heart. Taken together, these observations are suggestive for a role for Follistatin in cardiac valvulogenesis and a role for Follistatin-like1 in controlling late heart muscle cell formation.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="50021642"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="50021642"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 50021642; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=50021642]").text(description); $(".js-view-count[data-work-id=50021642]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 50021642; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='50021642']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 50021642, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=50021642]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":50021642,"title":"Patterns of Expression of the Follistatin and Follistatin-Like1 Genes During Chicken Heart Development: A Potential Role in Valvulogenesis and Late Heart Muscle Cell Formation","translated_title":"","metadata":{"abstract":"The regulation of concentration and function of growth factors is of crucial importance to proper embryonic development of the heart. The patterns of expression of three extracellular modulators of the transforming growth factor-beta superfamily of growth factors, Follistatin, Follistatin-like1, and Follistatin-like3, are described with respect to heart development. Follistatin is highly localized in the endocardium covering the developing cardiac valves. Follistatin-like1 is localized in the mesenchymal filling of the pharyngeal arches and broadly expressed in cells directly bordering myocardium. Follistatin-like3 is not expressed in the heart. 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Follistatin-like1 is localized in the mesenchymal filling of the pharyngeal arches and broadly expressed in cells directly bordering myocardium. Follistatin-like3 is not expressed in the heart. 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Antoon FM Moorman a , 1 , Cees A. Schumacher a , Piet AJ de Boer a , Jaco Hagoort a , Karel B...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... Antoon FM Moorman a , 1 , Cees A. Schumacher a , Piet AJ de Boer a , Jaco Hagoort a , Karel Bezstarosti b , Maurice JB van den Hoff a , Gerry TM Wagenaar a , Jos MJ Lamers b ... V. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="47408714"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/47408714/Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System"><img alt="Research paper thumbnail of Identifying the Evolutionary Building Blocks of the Cardiac Conduction System" class="work-thumbnail" src="https://attachments.academia-assets.com/66515100/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/47408714/Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System">Identifying the Evolutionary Building Blocks of the Cardiac Conduction System</a></div><div class="wp-workCard_item"><span>PLoS ONE</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a99b0acc42da9dfb0e9cd75dc5ac18dd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":66515100,"asset_id":47408714,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/66515100/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="47408714"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="47408714"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 47408714; 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These high heart rates are driven by very similar conduction systems consisting of an atrioventricular node that slows the electrical impulse and a His-Purkinje system that efficiently activates the ventricular chambers. While ectothermic vertebrates have similar contraction patterns, they do not possess anatomical evidence for a conduction system. This lack amongst extant ectotherms is surprising because mammals and birds evolved independently from reptilelike ancestors. Using conserved genetic markers, we found that the conduction system design of lizard (Anolis carolinensis and A. sagrei), frog (Xenopus laevis) and zebrafish (Danio rerio) adults is strikingly similar to that of embryos of mammals (mouse Mus musculus, and man) and chicken (Gallus gallus). Thus, in ectothermic adults, the slow conducting atrioventricular canal muscle is present, no fibrous insulating plane is formed, and the spongy ventricle serves the dual purpose of conduction and contraction. Optical mapping showed base-to-apex activation of the ventricles of the ectothermic animals, similar to the activation pattern of mammalian and avian embryonic ventricles and to the His-Purkinje systems of the formed hearts. Mammalian and avian ventricles uniquely develop thick compact walls and septum and, hence, form a discrete ventricular conduction system from the embryonic spongy ventricle. Our study uncovers the evolutionary building plan of heart and indicates that the building blocks of the conduction system of adult ectothermic vertebrates and embryos of endotherms are similar.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"PLoS ONE","grobid_abstract_attachment_id":66515100},"translated_abstract":null,"internal_url":"https://www.academia.edu/47408714/Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System","translated_internal_url":"","created_at":"2021-04-22T01:06:46.629-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":66515100,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/66515100/thumbnails/1.jpg","file_name":"00b7d51d47f1fd5bba000000.pdf","download_url":"https://www.academia.edu/attachments/66515100/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Identifying_the_Evolutionary_Building_Bl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/66515100/00b7d51d47f1fd5bba000000.pdf?1619078902=\u0026response-content-disposition=attachment%3B+filename%3DIdentifying_the_Evolutionary_Building_Bl.pdf\u0026Expires=1732831242\u0026Signature=FWhaKvdl4TD0tFC6OzKJeTZyff0MLWGRHB6SLl5KJiv9XtkDSIHUe5cGzdxEc-OOhl0BXibAnl7M5VwJJMWccGEk6EdoRlUvgaFqggtZaz9fhEWlGk2rL7uKIWcPjyXXv14v5rCli7kG6mKo9-vKO4z1K80ZclO2AKU8qk8DmqF08Hy0Coh~Y7-hBvru2SZ4OvD5eRDj~qUgJyNWJkxRL6ZlR2b7axwrMZCrA1FqBl1qVLTPCClLEgTXOU5qGM7UywSjdpuXxYlJu1yAzHRxFFq8BPumaalCXh7UVrdJHcVPj3p9-jhZdQ5F~RgRFz6HY~s3AC96D6Ao~LOJgXomug__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Identifying_the_Evolutionary_Building_Blocks_of_the_Cardiac_Conduction_System","translated_slug":"","page_count":13,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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Moorman</a></span></div><div class="wp-workCard_item"><span>Circulation Research</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7f2b686d0b4f9c2b3fdc5845d338260b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":54004524,"asset_id":34068769,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/54004524/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="34068769"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="34068769"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 34068769; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=34068769]").text(description); $(".js-view-count[data-work-id=34068769]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 34068769; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='34068769']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 34068769, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7f2b686d0b4f9c2b3fdc5845d338260b" } } $('.js-work-strip[data-work-id=34068769]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":34068769,"title":"Pitx2c and Nkx2-5 Are Required for the Formation and Identity of the Pulmonary Myocardium","translated_title":"","metadata":{"grobid_abstract":"The pulmonary vein is sleeved by myocardium, which is a major source of atrial fibrillation and is involved in congenital sinus venosus defects. Little is known about the cellular origin and mechanism of formation of the pulmonary myocardium. We observed a biphasic process of pulmonary myocardium formation in mice. Firstly, a myocardial cell population forms de novo at the connection of the pulmonary vein and the atrium. Genetic labeling revealed that atrial cells do not contribute to this population, indicating it forms by differentiation of pulmonary mesenchymal cells. Secondly, these pulmonary myocardial cells initiate a phase of rapid proliferation and form the pulmonary myocardial sleeve. Pitx2c-deficient mice do not develop a pulmonary myocardial sleeve because they fail to form the initial pulmonary myocardial cells. Genetic-labeling analyses demonstrated that whereas the systemic venous return derives from Nkx2-5-negative precursors, the pulmonary myocardium derives from Nkx2-5-expressing precursors, indicating a distinct origin of the 2 venous systems. Nkx2-5 and its target gap-junction gene Cx40 are expressed in the atria and in the pulmonary myocardium but not in the systemic venous return, which expresses the essential pacemaker channel Hcn4. When Nkx2-5 protein level was lowered in a hypomorphic model, the pulmonary myocardium switched to a Cx40-negative, Hcn4-positive phenotype resembling that of the systemic venous return. In conclusion, our data suggest a cellular mechanism for pulmonary myocardium formation and highlight the key roles played by Pitx2c and Nkx2-5 in its formation and identity. 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Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://academicmedicalcentreuniversiteitvanamsterdam.academia.edu/MauriceVanDenHoff">Maurice Van Den Hoff</a></span></div><div class="wp-workCard_item"><span>The Anatomical record</span><span>, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber fo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. At HH9 cANF expression was fir...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="ff105af027464c9633b9b8a49b04261f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44548975,"asset_id":24218601,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44548975/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218601"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218601"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218601; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24218601]").text(description); $(".js-view-count[data-work-id=24218601]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24218601; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24218601']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 24218601, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "ff105af027464c9633b9b8a49b04261f" } } $('.js-work-strip[data-work-id=24218601]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24218601,"title":"Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken","translated_title":"","metadata":{"abstract":"In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. 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Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://academicmedicalcentreuniversiteitvanamsterdam.academia.edu/MauriceVanDenHoff">Maurice Van Den Hoff</a></span></div><div class="wp-workCard_item"><span>The Anatomical Record</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="0045561ecd970ac10f9c2a3874fe6fd4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44548974,"asset_id":24218600,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44548974/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218600"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218600"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218600; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24218600]").text(description); $(".js-view-count[data-work-id=24218600]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24218600; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24218600']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 24218600, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "0045561ecd970ac10f9c2a3874fe6fd4" } } $('.js-work-strip[data-work-id=24218600]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24218600,"title":"Expression of cVg1 mRNA during chicken embryonic development","translated_title":"","metadata":{"grobid_abstract":"Using degenerated PCR-primers to identify known and novel BMPs that are expressed in the developing chicken heart, we identified not only BMP2, -4, and -7 mRNA, but also the TGF␤ superfamily member cVg1. 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Anat Rec Part A 273A:603-608, 2003.","publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"The Anatomical Record","grobid_abstract_attachment_id":44548974},"translated_abstract":null,"internal_url":"https://www.academia.edu/24218600/Expression_of_cVg1_mRNA_during_chicken_embryonic_development","translated_internal_url":"","created_at":"2016-04-08T13:28:27.689-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18709398,"work_id":24218600,"tagging_user_id":46673754,"tagged_user_id":46881112,"co_author_invite_id":4263284,"email":"m***f@amc.nl","affiliation":"Academic Medical Centre/ Universiteit van Amsterdam","display_order":0,"name":"Maurice Van Den Hoff","title":"Expression of cVg1 mRNA during chicken embryonic development"}],"downloadable_attachments":[{"id":44548974,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/44548974/thumbnails/1.jpg","file_name":"Expression_of_cVg1_mRNA_during_chicken_e20160408-1443-1q8euqc.pdf","download_url":"https://www.academia.edu/attachments/44548974/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Expression_of_cVg1_mRNA_during_chicken_e.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/44548974/Expression_of_cVg1_mRNA_during_chicken_e20160408-1443-1q8euqc-libre.pdf?1460147538=\u0026response-content-disposition=attachment%3B+filename%3DExpression_of_cVg1_mRNA_during_chicken_e.pdf\u0026Expires=1732831242\u0026Signature=Gsgvb-WV82aJMUSo4GaHMAZwJ~eHCda-iRmeKiOl~mXxtSnCwoBUgfZ41UsU~LGnrs-JyOBjmyS1OqhKlMD-j5yK8jSXR3Rf2dQird2QO6xs~tfkbiQMVklt4EuE4BThsKPes-6-qTIQxxkrA1j~cUaITU~~POhrvzwL4wA0NGY9f4sp8d6xMkPx-EAfirr5wl7xwJqZ8jpWUlgGs9URSA4GF4IRHBMm6fRliyX5FdwSUgR2XZ1rNvJNWt~wuwVPRvzZtsJYO7BOhJ8O5bbswjIZlXK2Du5C9X~RG5W32si6QlTCtkx2pbWWRPOxQsnVLvcgrcSMgWf6Jv39q~-f3g__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Expression_of_cVg1_mRNA_during_chicken_embryonic_development","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. 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Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://academicmedicalcentreuniversiteitvanamsterdam.academia.edu/MauriceVanDenHoff">Maurice Van Den Hoff</a></span></div><div class="wp-workCard_item"><span>Journal of Histochemistry and Cytochemistry</span><span>, 2006</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="895f76ac001c7fb13091cbcb08de23bd" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":44548973,"asset_id":24218599,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/44548973/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218599"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218599"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218599; 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Irx1 and Irx2 were found to be expressed specifically in the ventricular septum from the onset of its formation onward. In fetal stages, the expression of both genes appeared to gradually become confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Irx3 was found to be expressed specifically in the trabeculated myocardium of the ventricles. Irx4 expression was observed in a segment of the linear heart tube and the atrioventricular canal and ventricular myocardium including the inner curvature after looping, resembling the pattern of MLC2V. Transcripts for Irx5 were detected specifically in the endocardium lining the ventricular and atrial working myocardium that also expressed von Willebrand factor, but were absent from the endocardium of the endocardial cushions, i.e., the atrioventricular canal, inner curvature, and outflow tract. The spatiodevelopmental pattern of Irx5 matched that of ANF, a marker for the forming working myocardium of the chambers. Taken together, all members of the Irx gene family were found to be expressed in highly specific patterns in the developing mouse heart, suggesting a critical role in the specification of the distinct components of the four-chambered heart.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="24218598"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="24218598"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24218598; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24218598]").text(description); $(".js-view-count[data-work-id=24218598]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 24218598; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='24218598']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 24218598, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=24218598]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":24218598,"title":"Patterning the Embryonic Heart: Identification of Five Mouse Iroquois Homeobox Genes in the Developing Heart","translated_title":"","metadata":{"abstract":"We isolated cDNAs of mouse Iroquois-related homeobox genes Irx1, -2, -3, -4, and -5 and characterized their patterns of expression in the developing heart. Irx1 and Irx2 were found to be expressed specifically in the ventricular septum from the onset of its formation onward. In fetal stages, the expression of both genes appeared to gradually become confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Irx3 was found to be expressed specifically in the trabeculated myocardium of the ventricles. Irx4 expression was observed in a segment of the linear heart tube and the atrioventricular canal and ventricular myocardium including the inner curvature after looping, resembling the pattern of MLC2V. Transcripts for Irx5 were detected specifically in the endocardium lining the ventricular and atrial working myocardium that also expressed von Willebrand factor, but were absent from the endocardium of the endocardial cushions, i.e., the atrioventricular canal, inner curvature, and outflow tract. The spatiodevelopmental pattern of Irx5 matched that of ANF, a marker for the forming working myocardium of the chambers. Taken together, all members of the Irx gene family were found to be expressed in highly specific patterns in the developing mouse heart, suggesting a critical role in the specification of the distinct components of the four-chambered heart.","publication_date":{"day":null,"month":null,"year":2000,"errors":{}},"publication_name":"Developmental Biology"},"translated_abstract":"We isolated cDNAs of mouse Iroquois-related homeobox genes Irx1, -2, -3, -4, and -5 and characterized their patterns of expression in the developing heart. Irx1 and Irx2 were found to be expressed specifically in the ventricular septum from the onset of its formation onward. In fetal stages, the expression of both genes appeared to gradually become confined to the myocardium of the atrioventricular bundle and bundle branches of the forming ventricular conduction system. Irx3 was found to be expressed specifically in the trabeculated myocardium of the ventricles. Irx4 expression was observed in a segment of the linear heart tube and the atrioventricular canal and ventricular myocardium including the inner curvature after looping, resembling the pattern of MLC2V. Transcripts for Irx5 were detected specifically in the endocardium lining the ventricular and atrial working myocardium that also expressed von Willebrand factor, but were absent from the endocardium of the endocardial cushions, i.e., the atrioventricular canal, inner curvature, and outflow tract. The spatiodevelopmental pattern of Irx5 matched that of ANF, a marker for the forming working myocardium of the chambers. Taken together, all members of the Irx gene family were found to be expressed in highly specific patterns in the developing mouse heart, suggesting a critical role in the specification of the distinct components of the four-chambered heart.","internal_url":"https://www.academia.edu/24218598/Patterning_the_Embryonic_Heart_Identification_of_Five_Mouse_Iroquois_Homeobox_Genes_in_the_Developing_Heart","translated_internal_url":"","created_at":"2016-04-08T13:28:27.278-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":46673754,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":18709401,"work_id":24218598,"tagging_user_id":46673754,"tagged_user_id":null,"co_author_invite_id":4263285,"email":"a***n@amc.nl","display_order":0,"name":"Anton Morman","title":"Patterning the Embryonic Heart: Identification of Five Mouse Iroquois Homeobox Genes in the Developing Heart"}],"downloadable_attachments":[],"slug":"Patterning_the_Embryonic_Heart_Identification_of_Five_Mouse_Iroquois_Homeobox_Genes_in_the_Developing_Heart","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":46673754,"first_name":"A.","middle_initials":null,"last_name":"Moorman","page_name":"AMoorman1","domain_name":"independent","created_at":"2016-04-08T13:27:11.512-07:00","display_name":"A. Moorman","url":"https://independent.academia.edu/AMoorman1"},"attachments":[],"research_interests":[{"id":1083,"name":"Developmental Biology","url":"https://www.academia.edu/Documents/in/Developmental_Biology"},{"id":23323,"name":"Transcription Factors","url":"https://www.academia.edu/Documents/in/Transcription_Factors"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":80043,"name":"von Willebrand factor","url":"https://www.academia.edu/Documents/in/von_Willebrand_factor"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice"},{"id":131495,"name":"Heart","url":"https://www.academia.edu/Documents/in/Heart"},{"id":229238,"name":"Developmental","url":"https://www.academia.edu/Documents/in/Developmental"},{"id":370195,"name":"Body Patterning","url":"https://www.academia.edu/Documents/in/Body_Patterning"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence"},{"id":999803,"name":"Gene Family","url":"https://www.academia.edu/Documents/in/Gene_Family"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="16407867"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/16407867/Developmental_pattern_of_ANF_gene_expression_reveals_a_strict_localization_of_cardiac_chamber_formation_in_chicken"><img alt="Research paper thumbnail of Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken" class="work-thumbnail" src="https://attachments.academia-assets.com/42495653/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/16407867/Developmental_pattern_of_ANF_gene_expression_reveals_a_strict_localization_of_cardiac_chamber_formation_in_chicken">Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AMoorman1">A. Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ArjanHouweling">Arjan Houweling</a></span></div><div class="wp-workCard_item"><span>The Anatomical record</span><span>, 2002</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber fo...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. To gain insight into the process of chamber formation in the chicken embryonic heart, we analyzed the expression pattern of cANF during development. We found cANF to be specifically expressed in the myocardium of the morphologically distinguishable atrial and ventricular chambers, similar to ANF in mouse. cANF expression was never detected in the myocardium of the atrioventricular canal (AVC), inner curvature, and outflow tract (OFT), which is lined by endocardial cushions. Expression was strictly excluded from the interventricular myocardium and most proximal part of the bundle branches, as identified by the expression of Msx-2, whereas the rest of the bundle branches, trabeculae, and surrounding working myocardium did express cANF. The myocardium that forms de novo within the cushions after looping did not express cANF. At HH9 cANF expression was fir...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b1d944f0107e758961898e99c22ddd47" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42495653,"asset_id":16407867,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42495653/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="16407867"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="16407867"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16407867; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16407867]").text(description); $(".js-view-count[data-work-id=16407867]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16407867; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='16407867']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 16407867, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b1d944f0107e758961898e99c22ddd47" } } $('.js-work-strip[data-work-id=16407867]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":16407867,"title":"Developmental pattern of ANF gene expression reveals a strict localization of cardiac chamber formation in chicken","translated_title":"","metadata":{"abstract":"In mouse, atrial natriuretic factor (ANF) gene expression was shown to be a marker for chamber formation within the embryonic heart. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="16407858"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/16407858/Expression_of_cVg1_mRNA_during_chicken_embryonic_development"><img alt="Research paper thumbnail of Expression of cVg1 mRNA during chicken embryonic development" class="work-thumbnail" src="https://attachments.academia-assets.com/42495646/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/16407858/Expression_of_cVg1_mRNA_during_chicken_embryonic_development">Expression of cVg1 mRNA during chicken embryonic development</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AMoorman1">A. Moorman</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ArjanHouweling">Arjan Houweling</a></span></div><div class="wp-workCard_item"><span>The Anatomical Record</span><span>, 2003</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fa99aece7ac686f5a10a50bb690c055f" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42495646,"asset_id":16407858,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42495646/download_file?st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&st=MTczMjgyNzY0Miw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="16407858"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="16407858"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16407858; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16407858]").text(description); $(".js-view-count[data-work-id=16407858]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16407858; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='16407858']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 16407858, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fa99aece7ac686f5a10a50bb690c055f" } } $('.js-work-strip[data-work-id=16407858]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":16407858,"title":"Expression of cVg1 mRNA during chicken embryonic development","translated_title":"","metadata":{"grobid_abstract":"Using degenerated PCR-primers to identify known and novel BMPs that are expressed in the developing chicken heart, we identified not only BMP2, -4, and -7 mRNA, but also the TGF␤ superfamily member cVg1. The expression pattern of cVg1 mRNA was determined during chicken development from HH4 to HH44. In early developmental stages, cVg1 mRNA is expressed in the primitive streak, paraxial mesoderm, developing somites, and developing neural tube. Subsequently, cVg1 mRNA is expressed in the developing central and peripheral nervous system, retina, auditory vesicle, notochord, lung alveoli, and olfactory mucosa. In the heart, cVg1 is initially expressed through the linear heart tube, but becomes restricted to the forming chamber myocardium, in an expression domain similar to that of atrial natriuretic factor (ANF) mRNA. Anat Rec Part A 273A:603-608, 2003.","publication_date":{"day":null,"month":null,"year":2003,"errors":{}},"publication_name":"The Anatomical Record","grobid_abstract_attachment_id":42495646},"translated_abstract":null,"internal_url":"https://www.academia.edu/16407858/Expression_of_cVg1_mRNA_during_chicken_embryonic_development","translated_internal_url":"","created_at":"2015-10-02T10:39:20.793-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":35550583,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":6591347,"work_id":16407858,"tagging_user_id":35550583,"tagged_user_id":46673754,"co_author_invite_id":1468379,"email":"a***n@amc.uva.nl","display_order":0,"name":"A. 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