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class="js-work-strip profile--work_container" data-work-id="36737964"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737964/Humanization_and_simultaneous_optimization_of_monoclonal_antibody"><img alt="Research paper thumbnail of Humanization and simultaneous optimization of monoclonal antibody" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737964/Humanization_and_simultaneous_optimization_of_monoclonal_antibody">Humanization and simultaneous optimization of monoclonal antibody</a></div><div class="wp-workCard_item"><span>Methods in molecular biology (Clifton, N.J.)</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antibody humanization is an essential technology for reducing the potential risk of immunogenicit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antibody humanization is an essential technology for reducing the potential risk of immunogenicity associated with animal-derived antibodies and has been applied to a majority of the therapeutic antibodies on the market. For developing an antibody molecule as a pharmaceutical at the current biotechnology level, however, other properties also have to be considered in parallel with humanization in antibody generation and optimization. This section describes the critical properties of therapeutic antibodies that should be sufficiently qualified, including immunogenicity, binding affinity, physiochemical stability, expression in host cells and pharmacokinetics, and the basic methodologies of antibody engineering involved. By simultaneously optimizing the antibody molecule in the light of these properties, it should prove possible to shorten the research and development period necessary to identify a highly qualified clinical candidate and consequently accelerate the start of the clinica...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737964"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737964"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737964; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737964]").text(description); $(".js-view-count[data-work-id=36737964]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737964; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737964']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737964, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=36737964]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737964,"title":"Humanization and simultaneous optimization of monoclonal antibody","translated_title":"","metadata":{"abstract":"Antibody humanization is an essential technology for reducing the potential risk of immunogenicity associated with animal-derived antibodies and has been applied to a majority of the therapeutic antibodies on the market. For developing an antibody molecule as a pharmaceutical at the current biotechnology level, however, other properties also have to be considered in parallel with humanization in antibody generation and optimization. This section describes the critical properties of therapeutic antibodies that should be sufficiently qualified, including immunogenicity, binding affinity, physiochemical stability, expression in host cells and pharmacokinetics, and the basic methodologies of antibody engineering involved. 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This section describes the critical properties of therapeutic antibodies that should be sufficiently qualified, including immunogenicity, binding affinity, physiochemical stability, expression in host cells and pharmacokinetics, and the basic methodologies of antibody engineering involved. By simultaneously optimizing the antibody molecule in the light of these properties, it should prove possible to shorten the research and development period necessary to identify a highly qualified clinical candidate and consequently accelerate the start of the clinica...","internal_url":"https://www.academia.edu/36737964/Humanization_and_simultaneous_optimization_of_monoclonal_antibody","translated_internal_url":"","created_at":"2018-05-29T01:56:41.414-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Humanization_and_simultaneous_optimization_of_monoclonal_antibody","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[],"research_interests":[{"id":31264,"name":"Protein Engineering","url":"https://www.academia.edu/Documents/in/Protein_Engineering"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); 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window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737963]").text(description); $(".js-view-count[data-work-id=36737963]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737963; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737963']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737963, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bfea9be1ad0641243dfd2d523fb19f46" } } $('.js-work-strip[data-work-id=36737963]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737963,"title":"MP-22.14: Prospective assessment of hot flashes during treatment with maximum androgen blockade using steroidal or non-steroidal antiandrogens for prostate cancer","translated_title":"","metadata":{"grobid_abstract":"Introduction: Androgen deprivation therapy for prostate cancer usually affects quality of life (QOL) in individual patients for long periods of time. Hot flashes, which are major adverse reactions to androgen deprivation therapy, can produce prolonged reductions in QOL. We recently conducted a 2-year longitudinal survey of the incidence, frequency and severity of hot flashes during maximum androgen blockade (MAB) therapy using steroidal or non-steroidal antiandrogens. Methods: A total of 151 patients with stage B-D prostate cancer, enrolled to this survey between May 2001 and June 2003, were randomized to receive MAB therapy using leuprorelin (LHRH agonist) and a steroidal antiandrogen, chlormadinone acetate (CMA group) or MAB using leuprorelin and a nonsteroidal antiandrogen, bicalutamide (BICA group). The incidence and frequency of, and distress due to hot flashes were evaluated with a self-entry questionnaire at baseline, and at 2-week intervals for the first 3 months, and then at 3-month intervals for 21 months. General and disease-specific QOL outcomes were measured with the Functional Assessment of Cancer Therapy (FACT) questionnaire at the same time. Results: Data were available for analysis on 124 patients. There was no difference in biochemical relapse-free survival at 2 years between CMA and BICA groups (88.0% vs. 84.4%, respectively). The incidence of hot flashes tended overall to be higher in BICA group than in CMA group. At 24 months, the incidence of flushing was 36.1% vs. 20.7%, and that of sweating was 33.3% vs. 21.4% in BICA and CMA groups, respectively. The frequency of hot flashes per day was higher in BICA Group than in CMA group (pϭ0.09 for flushing and pϭ0.021 for sweating). 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="25289246"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/25289246/Radiocesium_accumulation_in_the_anuran_frog_Rana_tagoi_tagoi_in_forest_ecosystems_after_the_Fukushima_Nuclear_Power_Plant_accident"><img alt="Research paper thumbnail of Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident" class="work-thumbnail" src="https://attachments.academia-assets.com/45590350/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/25289246/Radiocesium_accumulation_in_the_anuran_frog_Rana_tagoi_tagoi_in_forest_ecosystems_after_the_Fukushima_Nuclear_Power_Plant_accident">Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ToshinoriOkuda">Toshinori Okuda</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TIgawa">Takeshi Igawa</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TeruhikoTakahara">Teruhiko Takahara</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://hiroshima-u.academia.edu/SatoruEndo">Satoru Endo</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/YurikaOba">Yurika Oba</a></span></div><div class="wp-workCard_item"><span>Environmental pollution (Barking, Essex : 1987)</span><span>, Jan 29, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Amphibians are key components in forest food webs. When examining radioactive contamination in an...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Amphibians are key components in forest food webs. When examining radioactive contamination in anurans, it is important to understand how radiocesium transfer occurs from lower to higher trophic levels in forest ecosystems. We investigated the activity concentration of radiocesium ((134)Cs and (137)Cs) in Tago&#39;s brown frog (Rana tagoi tagoi) captured on the forest floor approximately 2.5 years after the Fukushima Nuclear Power Plant (FNPP) accident. We collected 66 R. tagoi tagoi at different distances from the FNPP. Radiocesium accumulation showed positive correlations with the air radiation dose rate and litter contamination but not with distance from the FNPP. Whole-body radioactivity showed no correlation with body mass or length. Our results suggest that differences in the available food items result in large variability in individual contamination. Contamination level monitoring in terrestrial and aquatic amphibian is necessary for clarifying the processes and mechanisms o...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="619a780c7a1646a7c5122224010c7873" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45590350,"asset_id":25289246,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45590350/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="25289246"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="25289246"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 25289246; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=25289246]").text(description); $(".js-view-count[data-work-id=25289246]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 25289246; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='25289246']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 25289246, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "619a780c7a1646a7c5122224010c7873" } } $('.js-work-strip[data-work-id=25289246]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":25289246,"title":"Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident","translated_title":"","metadata":{"abstract":"Amphibians are key components in forest food webs. When examining radioactive contamination in anurans, it is important to understand how radiocesium transfer occurs from lower to higher trophic levels in forest ecosystems. We investigated the activity concentration of radiocesium ((134)Cs and (137)Cs) in Tago\u0026#39;s brown frog (Rana tagoi tagoi) captured on the forest floor approximately 2.5 years after the Fukushima Nuclear Power Plant (FNPP) accident. We collected 66 R. tagoi tagoi at different distances from the FNPP. Radiocesium accumulation showed positive correlations with the air radiation dose rate and litter contamination but not with distance from the FNPP. Whole-body radioactivity showed no correlation with body mass or length. Our results suggest that differences in the available food items result in large variability in individual contamination. Contamination level monitoring in terrestrial and aquatic amphibian is necessary for clarifying the processes and mechanisms o...","publication_date":{"day":29,"month":1,"year":2015,"errors":{}},"publication_name":"Environmental pollution (Barking, Essex : 1987)"},"translated_abstract":"Amphibians are key components in forest food webs. When examining radioactive contamination in anurans, it is important to understand how radiocesium transfer occurs from lower to higher trophic levels in forest ecosystems. We investigated the activity concentration of radiocesium ((134)Cs and (137)Cs) in Tago\u0026#39;s brown frog (Rana tagoi tagoi) captured on the forest floor approximately 2.5 years after the Fukushima Nuclear Power Plant (FNPP) accident. We collected 66 R. tagoi tagoi at different distances from the FNPP. Radiocesium accumulation showed positive correlations with the air radiation dose rate and litter contamination but not with distance from the FNPP. Whole-body radioactivity showed no correlation with body mass or length. Our results suggest that differences in the available food items result in large variability in individual contamination. 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27451130"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27451130/Genomic_structure_and_evolution_of_multigene_families_flowers_on_the_human_genome"><img alt="Research paper thumbnail of Genomic structure and evolution of multigene families: "flowers" on the human genome" class="work-thumbnail" src="https://attachments.academia-assets.com/47710212/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27451130/Genomic_structure_and_evolution_of_multigene_families_flowers_on_the_human_genome">Genomic structure and evolution of multigene families: "flowers" on the human genome</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://nanyang.academia.edu/HieLimKim">Hie Lim Kim</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TIgawa">Takeshi Igawa</a></span></div><div class="wp-workCard_item"><span>International journal of evolutionary biology</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c9f4e524d47b9279ab97b3644aad7137" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":47710212,"asset_id":27451130,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/47710212/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa 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adjacent chromosomal regions. We named such structures \"Flowers\" because the pattern observed on dot plots resembles a flower. We detected a total of 291 Flowers in the human genome. They were predominantly located in euchromatic regions. Flowers are gene-rich compared to the average gene density of the genome. Genes involved in systems receiving environmental information, such as immunity and detoxification, were overrepresented in Flowers. Within a Flower, the mean number of duplication units was approximately four. The maximum and minimum identities between homologs in a Flower showed different distributions; the maximum identity was often concentrated to 100% identity, while the minimum identity was evenly distributed in the range of 78% to 100%. Using a gene conversion detection test, we found frequent and/or recent gene conversion events within the tested Flowers. Interestingly, many of those converted regions contained protein-coding genes. Computer simulation studies suggest that one role of such frequent gene conversions is the elongation of the life span of gene families in a Flower by the resurrection of pseudogenes.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"International journal of evolutionary biology","grobid_abstract_attachment_id":47710212},"translated_abstract":null,"internal_url":"https://www.academia.edu/27451130/Genomic_structure_and_evolution_of_multigene_families_flowers_on_the_human_genome","translated_internal_url":"","created_at":"2016-08-01T19:00:31.205-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34029428,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":22992649,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":51641042,"co_author_invite_id":1020839,"email":"t***a@hiroshima-u.ac.jp","display_order":0,"name":"Takeshi Igawa","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992651,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":null,"co_author_invite_id":5129975,"email":"i***o@soken.ac.jp","display_order":4194304,"name":"Mineyo Iwase","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992653,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":null,"co_author_invite_id":5129976,"email":"k***o@soken.ac.jp","display_order":6291456,"name":"Satoko Kaneko","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992659,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":36880064,"co_author_invite_id":null,"email":"s***a@soken.ac.jp","display_order":7340032,"name":"Yoko Satta","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992662,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":38302016,"co_author_invite_id":null,"email":"t***a@soken.ac.jp","display_order":7864320,"name":"N. Takahata","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"}],"downloadable_attachments":[{"id":47710212,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/47710212/thumbnails/1.jpg","file_name":"Genomic_structure_and_evolution_of_multi20160801-29792-n78ce6.pdf","download_url":"https://www.academia.edu/attachments/47710212/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genomic_structure_and_evolution_of_multi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/47710212/Genomic_structure_and_evolution_of_multi20160801-29792-n78ce6-libre.pdf?1470103764=\u0026response-content-disposition=attachment%3B+filename%3DGenomic_structure_and_evolution_of_multi.pdf\u0026Expires=1732782714\u0026Signature=RtuaZMu2qd~Wt5AMaaDhP7drAwxP-g0jabNByyvH145Hk2iL2paU1alHk176xCNyhp3Ao6nb01cQ7VxIqThs7x9xHrx1ZvMNcROtln8KnJ4wVfvQLfnReoB7OUj5xVKxAVg325fRQPIdLfFVYqXyAVQF6VvjZCFJVgS421SsMnLI53V5XvICPhVJv37lvTE0XDFEylSvhKiiCjMzYNROnU76id1p0TSmP58Cfcn9pxsWtDud0RQ87qWuBKsAHk2WtmfSLg-hbQxdBBNvzBbj5kjtzqkcNlb~Q3-M3a3zKKZFamKh4bPoYngvRHoIpNhCTokB59kQA5PyO3enCT678w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Genomic_structure_and_evolution_of_multigene_families_flowers_on_the_human_genome","translated_slug":"","page_count":11,"language":"en","content_type":"Work","owner":{"id":34029428,"first_name":"Hie Lim","middle_initials":null,"last_name":"Kim","page_name":"HieLimKim","domain_name":"nanyang","created_at":"2015-08-18T19:43:02.935-07:00","display_name":"Hie Lim Kim","url":"https://nanyang.academia.edu/HieLimKim"},"attachments":[{"id":47710212,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/47710212/thumbnails/1.jpg","file_name":"Genomic_structure_and_evolution_of_multi20160801-29792-n78ce6.pdf","download_url":"https://www.academia.edu/attachments/47710212/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Genomic_structure_and_evolution_of_multi.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/47710212/Genomic_structure_and_evolution_of_multi20160801-29792-n78ce6-libre.pdf?1470103764=\u0026response-content-disposition=attachment%3B+filename%3DGenomic_structure_and_evolution_of_multi.pdf\u0026Expires=1732782714\u0026Signature=RtuaZMu2qd~Wt5AMaaDhP7drAwxP-g0jabNByyvH145Hk2iL2paU1alHk176xCNyhp3Ao6nb01cQ7VxIqThs7x9xHrx1ZvMNcROtln8KnJ4wVfvQLfnReoB7OUj5xVKxAVg325fRQPIdLfFVYqXyAVQF6VvjZCFJVgS421SsMnLI53V5XvICPhVJv37lvTE0XDFEylSvhKiiCjMzYNROnU76id1p0TSmP58Cfcn9pxsWtDud0RQ87qWuBKsAHk2WtmfSLg-hbQxdBBNvzBbj5kjtzqkcNlb~Q3-M3a3zKKZFamKh4bPoYngvRHoIpNhCTokB59kQA5PyO3enCT678w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":155,"name":"Evolutionary Biology","url":"https://www.academia.edu/Documents/in/Evolutionary_Biology"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737958"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737958/Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs"><img alt="Research paper thumbnail of Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737958/Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs">Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs</a></div><div class="wp-workCard_item"><span>Journal of Heredity</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis, Odorrana supranarina, and Odorrana utsunomiyaorum, belong to the family Ranidae and are endemically distributed in Okinawa (O. narina), Amami and Tokunoshima (O. amamiensis), and Ishigaki and Iriomote (O. supranarina and O. utsunomiyaorum) Islands. Because of varying distribution patterns, this species complex is an intrinsic model for speciation and adaptation. For effective conservation and molecular ecological studies, further genetic information is needed. For rapid, cost-effective development of several microsatellite markers for these and 2 other species, we used next-generation sequencing technology of Ion Torrent PGM™. Distribution patterns of repeat motifs of microsatellite loci in these modern frog species (Neobatrachia) were similarly skewed. We isolated and characterized 20 new microsatellite loci of O. narina and validated cross-amplification in the three-related species. Seventeen, 16, and 13 loci were cross-amplified in O. amamiensis, O. supranarina, and O. utsunomiyaorum, respectively, reflecting close genetic relationships between them. Mean number of alleles and expected heterozygosity of newly isolated loci varied depending on the size of each inhabited island. Our findings suggested the suitability of Ion Torrent PGM™ for microsatellite marker development. The new markers developed for the O. narina complex will be applicable in conservation genetics and molecular ecological studies.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737958"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737958"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737958; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737958]").text(description); $(".js-view-count[data-work-id=36737958]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737958; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737958']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737958, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=36737958]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737958,"title":"Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs","translated_title":"","metadata":{"abstract":"The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis, Odorrana supranarina, and Odorrana utsunomiyaorum, belong to the family Ranidae and are endemically distributed in Okinawa (O. narina), Amami and Tokunoshima (O. amamiensis), and Ishigaki and Iriomote (O. supranarina and O. utsunomiyaorum) Islands. Because of varying distribution patterns, this species complex is an intrinsic model for speciation and adaptation. For effective conservation and molecular ecological studies, further genetic information is needed. For rapid, cost-effective development of several microsatellite markers for these and 2 other species, we used next-generation sequencing technology of Ion Torrent PGM™. Distribution patterns of repeat motifs of microsatellite loci in these modern frog species (Neobatrachia) were similarly skewed. We isolated and characterized 20 new microsatellite loci of O. narina and validated cross-amplification in the three-related species. Seventeen, 16, and 13 loci were cross-amplified in O. amamiensis, O. supranarina, and O. utsunomiyaorum, respectively, reflecting close genetic relationships between them. Mean number of alleles and expected heterozygosity of newly isolated loci varied depending on the size of each inhabited island. Our findings suggested the suitability of Ion Torrent PGM™ for microsatellite marker development. The new markers developed for the O. narina complex will be applicable in conservation genetics and molecular ecological studies.","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Journal of Heredity"},"translated_abstract":"The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis, Odorrana supranarina, and Odorrana utsunomiyaorum, belong to the family Ranidae and are endemically distributed in Okinawa (O. narina), Amami and Tokunoshima (O. amamiensis), and Ishigaki and Iriomote (O. supranarina and O. utsunomiyaorum) Islands. Because of varying distribution patterns, this species complex is an intrinsic model for speciation and adaptation. For effective conservation and molecular ecological studies, further genetic information is needed. For rapid, cost-effective development of several microsatellite markers for these and 2 other species, we used next-generation sequencing technology of Ion Torrent PGM™. Distribution patterns of repeat motifs of microsatellite loci in these modern frog species (Neobatrachia) were similarly skewed. We isolated and characterized 20 new microsatellite loci of O. narina and validated cross-amplification in the three-related species. Seventeen, 16, and 13 loci were cross-amplified in O. amamiensis, O. supranarina, and O. utsunomiyaorum, respectively, reflecting close genetic relationships between them. Mean number of alleles and expected heterozygosity of newly isolated loci varied depending on the size of each inhabited island. Our findings suggested the suitability of Ion Torrent PGM™ for microsatellite marker development. The new markers developed for the O. narina complex will be applicable in conservation genetics and molecular ecological studies.","internal_url":"https://www.academia.edu/36737958/Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs","translated_internal_url":"","created_at":"2018-05-29T01:56:40.370-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":17158,"name":"Japan","url":"https://www.academia.edu/Documents/in/Japan"},{"id":23979,"name":"Endangered Species","url":"https://www.academia.edu/Documents/in/Endangered_Species"},{"id":42324,"name":"Heredity","url":"https://www.academia.edu/Documents/in/Heredity"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype"},{"id":426361,"name":"Ranidae","url":"https://www.academia.edu/Documents/in/Ranidae"},{"id":569436,"name":"Genomic Library","url":"https://www.academia.edu/Documents/in/Genomic_Library"},{"id":719974,"name":"Conservation of Natural Resources","url":"https://www.academia.edu/Documents/in/Conservation_of_Natural_Resources"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity"},{"id":1232430,"name":"Genetic Markers","url":"https://www.academia.edu/Documents/in/Genetic_Markers"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737957"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737957/Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments"><img alt="Research paper thumbnail of Evolutionary Relationships and Reproductive Isolating Mechanisms in the Rice Frog (Fejervarya limnocharis) Species Complex from Sri Lanka, Thailand, Taiwan and Japan, Inferred from mtDNA Gene Sequences, Allozymes, and Crossing Experiments" class="work-thumbnail" src="https://attachments.academia-assets.com/56681077/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737957/Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments">Evolutionary Relationships and Reproductive Isolating Mechanisms in the Rice Frog (Fejervarya limnocharis) Species Complex from Sri Lanka, Thailand, Taiwan and Japan, Inferred from mtDNA Gene Sequences, Allozymes, and Crossing Experiments</a></div><div class="wp-workCard_item"><span>Zoological Science</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="429d92b433584e7cfabc06b67c382174" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56681077,"asset_id":36737957,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56681077/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737957"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737957"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737957; 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Conspicuous morphological variation has been reported for this species complex throughout its distribution range. In the present study, we used mtDNA gene sequence and allozyme analyses to infer evolutionary affinities within this species complex using eight populations (Sri Lanka; Bangkok and Ranong in Thailand; Taiwan; and Hiroshima, Okinawa, Ishigaki and Iriomote in Japan). We also conducted crossing experiments among four populations from Japan, Thailand, and Sri Lanka in order to find out more about the reproductive isolating mechanisms that might exist among the East, Southeast, and South Asian populations of this species complex. The crossing experiments revealed that the Sri Lanka population is reproductively isolated from the Hiroshima, Bangkok, and Ranong populations by complete hybrid inviability, and that the Bangkok population may be reproductively isolated from the Hiroshima population by partial hybrid inviability. Thus, it is not unreasonable to regard the Sri Lanka population as a species separated from F. limnocharis. The mtDNA and allozyme data showed that the Ranong population is most closely related to the Bangkok population in nuclear genome, but more similar to the Okinawa and Taiwan populations in mtDNA genome. The present, preliminary survey may raise questions about the species status of these particular populations and also about the nature of the biological species concept.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Zoological Science","grobid_abstract_attachment_id":56681077},"translated_abstract":null,"internal_url":"https://www.academia.edu/36737957/Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments","translated_internal_url":"","created_at":"2018-05-29T01:56:40.267-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":56681077,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681077/thumbnails/1.jpg","file_name":"0fcfd51205a10d7dd1000000.pdf","download_url":"https://www.academia.edu/attachments/56681077/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Evolutionary_Relationships_and_Reproduct.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681077/0fcfd51205a10d7dd1000000.pdf?1527584266=\u0026response-content-disposition=attachment%3B+filename%3DEvolutionary_Relationships_and_Reproduct.pdf\u0026Expires=1732782714\u0026Signature=A0STWAUYbRDjx5i~fzL36jZNYA-uASwXp-hVMdac4AMGDdm5dFLpnWOAQsOKD0trlMSQdHIADHbbsv1jVkqm0-irH7orTznDInrEVQsSa4TmV1tv4NrnNoypvOTNf4Alr1oYX-i61GSQY1ZZVzSFTDipKIP7wDrv6TRfM78RdXuQS843FP5FbwLrwTj5m~EKz8Eg6EJRDzbRLnuRKNKmK~Cx2cVrPGKCf~CZq3chGNkzEH2jb9uUoATZ4uxj75T9V02NljV-aee7~LWBuwbK8xiEXqw-MY35U4-bWOU7nyBPbvgJbJxo3FWaEHJ2AcxKwDT4Df-tk12z9j6iQJ3gXw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments","translated_slug":"","page_count":17,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi 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Evolution","url":"https://www.academia.edu/Documents/in/Molecular_Evolution"},{"id":9850,"name":"Sri Lanka","url":"https://www.academia.edu/Documents/in/Sri_Lanka"},{"id":10096,"name":"Thailand","url":"https://www.academia.edu/Documents/in/Thailand"},{"id":17158,"name":"Japan","url":"https://www.academia.edu/Documents/in/Japan"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":55163,"name":"Enzymes","url":"https://www.academia.edu/Documents/in/Enzymes"},{"id":63093,"name":"Mitochondrial DNA","url":"https://www.academia.edu/Documents/in/Mitochondrial_DNA"},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment"},{"id":86952,"name":"Haplotypes","url":"https://www.academia.edu/Documents/in/Haplotypes"},{"id":94451,"name":"Taiwan","url":"https://www.academia.edu/Documents/in/Taiwan"},{"id":358670,"name":"Reproductive Isolation","url":"https://www.academia.edu/Documents/in/Reproductive_Isolation"},{"id":426361,"name":"Ranidae","url":"https://www.academia.edu/Documents/in/Ranidae"},{"id":577933,"name":"Genetic variation","url":"https://www.academia.edu/Documents/in/Genetic_variation"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737956"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737956/An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan"><img alt="Research paper thumbnail of An Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan" class="work-thumbnail" src="https://attachments.academia-assets.com/56681079/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737956/An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan">An Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan</a></div><div class="wp-workCard_item"><span>Animals</span><span>, 2013</span></div><div class="wp-workCard_item 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Echinotriton andersoni, from the Central Ryukyus in Japan","translated_title":"","metadata":{"grobid_abstract":"Simple Summary: We naturally bred the endangered Anderson's crocodile newt (Echinotriton andersoni) and tested a laboratory farming technique using near-biotopic breeding cages with several male and female pairs collected from Okinawa, Amami, and Tokunoshima Islands. This is the first published report of successfully propagating an endangered species by using breeding cages in a laboratory setting for captive breeding. Our findings on the natural breeding and raising of larvae and adults are useful in breeding this endangered species, and can be applied to the preservation of other similarly wild and endangered species.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Animals","grobid_abstract_attachment_id":56681079},"translated_abstract":null,"internal_url":"https://www.academia.edu/36737956/An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan","translated_internal_url":"","created_at":"2018-05-29T01:56:40.173-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":56681079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681079/thumbnails/1.jpg","file_name":"animals-03-00680.pdf","download_url":"https://www.academia.edu/attachments/56681079/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"An_Attempt_at_Captive_Breeding_of_the_En.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681079/animals-03-00680-libre.pdf?1527584624=\u0026response-content-disposition=attachment%3B+filename%3DAn_Attempt_at_Captive_Breeding_of_the_En.pdf\u0026Expires=1732782714\u0026Signature=S8KSezzM1bT9a-Yz3iTkYA5jyBN1KVwKySNk4i8xbv4H2Qfojqf3Ll7Xx0m3zmghsCv-loLxjyu0jYvk1U5Ho5-nV6hCY2yLldY46WKuGBlih36qs~q2liVAWvg5h1NSxXpNUtuILYnY0hqBtD-u3FH29EEo6TsSgP7XFPqj9kyGlDr1ixmEcP-036NvVWaCzikY8VoaU84~bvMl7nJA~ULIwx8y0P9a~7mnXoIr0TSWRwWlx5izD6Q0p3DBpYbHgL4FMrqD4pq47vi~PHx3sKQHEwt6yTIznRK3x0uongguhVXDjOzqCHXiu9krv4ao0CcQzsqRgaWcqtX01wkX~A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan","translated_slug":"","page_count":13,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[{"id":56681079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681079/thumbnails/1.jpg","file_name":"animals-03-00680.pdf","download_url":"https://www.academia.edu/attachments/56681079/download_file?st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"An_Attempt_at_Captive_Breeding_of_the_En.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681079/animals-03-00680-libre.pdf?1527584624=\u0026response-content-disposition=attachment%3B+filename%3DAn_Attempt_at_Captive_Breeding_of_the_En.pdf\u0026Expires=1732782714\u0026Signature=S8KSezzM1bT9a-Yz3iTkYA5jyBN1KVwKySNk4i8xbv4H2Qfojqf3Ll7Xx0m3zmghsCv-loLxjyu0jYvk1U5Ho5-nV6hCY2yLldY46WKuGBlih36qs~q2liVAWvg5h1NSxXpNUtuILYnY0hqBtD-u3FH29EEo6TsSgP7XFPqj9kyGlDr1ixmEcP-036NvVWaCzikY8VoaU84~bvMl7nJA~ULIwx8y0P9a~7mnXoIr0TSWRwWlx5izD6Q0p3DBpYbHgL4FMrqD4pq47vi~PHx3sKQHEwt6yTIznRK3x0uongguhVXDjOzqCHXiu9krv4ao0CcQzsqRgaWcqtX01wkX~A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":200081,"name":"IUCN Red List","url":"https://www.academia.edu/Documents/in/IUCN_Red_List"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737955"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737955/Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria"><img alt="Research paper thumbnail of Isolation and characterization of the plant glsA promoter from Alstroemeria" class="work-thumbnail" src="https://attachments.academia-assets.com/56681080/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737955/Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria">Isolation and characterization of the plant glsA promoter from Alstroemeria</a></div><div class="wp-workCard_item"><span>Plant Biology</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1dd6424661ce6a2976ab13e2baf9798e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56681080,"asset_id":36737955,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56681080/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737955"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737955"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737955; 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The Lilium GlsA is known to localize in pollen and is considered to be involved in development of the generative cell. Here, we cloned a glsA ortholog from Alstroemeria, a commercially important cut flower. The expression of AaglsA (Alstroemeria aurea glsA) transcripts increased gradually after pollen mitosis I (PMI) and reached a significant level when the generative cell started to elongate. Analysis of the promoter of AaglsA suggests that AaglsA expression is controlled by several cis-regulatory elements during pollen development. This is the first investigation of reproductive factors regulating male gametogenesis in Alstroemeria.","publication_date":{"day":null,"month":null,"year":2009,"errors":{}},"publication_name":"Plant Biology","grobid_abstract_attachment_id":56681080},"translated_abstract":null,"internal_url":"https://www.academia.edu/36737955/Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria","translated_internal_url":"","created_at":"2018-05-29T01:56:40.080-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":56681080,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681080/thumbnails/1.jpg","file_name":"j.1438-8677.2008.00177.x20180529-20264-7q96lv.pdf","download_url":"https://www.academia.edu/attachments/56681080/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Isolation_and_characterization_of_the_pl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681080/j.1438-8677.2008.00177.x20180529-20264-7q96lv-libre.pdf?1527584627=\u0026response-content-disposition=attachment%3B+filename%3DIsolation_and_characterization_of_the_pl.pdf\u0026Expires=1732782715\u0026Signature=Hbd4tpxojqZby680btD5IFeoV6K8P1ZFQc3-RwZ9-DdLR1cwXHQXUNplqW~dLdpMzZytxwkZqqhyiRiv4X-61Y5Sw2CPNNMAUOcyBQzvIWom3SdwShZqsU3WbNWrXXVNqOdtg5LWI067Uimh40p7j3QPhgEV4zmYoWz9IsPvbX74NPVi968j3rpvMgnUuZwCgFy22MXI6GuO7BVdFXaGr8Ty59VCNUU4bVNxHdr-Bvb3rSiUH-VwYySZSB-Y9HJIOn~GlDQEJtYJANgLL~7H7pwKgf-sClBj~fmtahuwdUq3gMf~2wV0qtxTPdEYlmMOcStv9bwe-Z5Lo~UT8b0~ew__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria","translated_slug":"","page_count":8,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[{"id":56681080,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681080/thumbnails/1.jpg","file_name":"j.1438-8677.2008.00177.x20180529-20264-7q96lv.pdf","download_url":"https://www.academia.edu/attachments/56681080/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Isolation_and_characterization_of_the_pl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681080/j.1438-8677.2008.00177.x20180529-20264-7q96lv-libre.pdf?1527584627=\u0026response-content-disposition=attachment%3B+filename%3DIsolation_and_characterization_of_the_pl.pdf\u0026Expires=1732782715\u0026Signature=Hbd4tpxojqZby680btD5IFeoV6K8P1ZFQc3-RwZ9-DdLR1cwXHQXUNplqW~dLdpMzZytxwkZqqhyiRiv4X-61Y5Sw2CPNNMAUOcyBQzvIWom3SdwShZqsU3WbNWrXXVNqOdtg5LWI067Uimh40p7j3QPhgEV4zmYoWz9IsPvbX74NPVi968j3rpvMgnUuZwCgFy22MXI6GuO7BVdFXaGr8Ty59VCNUU4bVNxHdr-Bvb3rSiUH-VwYySZSB-Y9HJIOn~GlDQEJtYJANgLL~7H7pwKgf-sClBj~fmtahuwdUq3gMf~2wV0qtxTPdEYlmMOcStv9bwe-Z5Lo~UT8b0~ew__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology"},{"id":55267,"name":"Mitosis","url":"https://www.academia.edu/Documents/in/Mitosis"},{"id":83086,"name":"Pollen","url":"https://www.academia.edu/Documents/in/Pollen"},{"id":121673,"name":"Promoter","url":"https://www.academia.edu/Documents/in/Promoter"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning"},{"id":305219,"name":"Alstroemeria","url":"https://www.academia.edu/Documents/in/Alstroemeria"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":1181939,"name":"PLANT PROTEINS","url":"https://www.academia.edu/Documents/in/PLANT_PROTEINS"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737954"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737954/Isolation_and_identification_of_ubiquitin_related_proteins_from_Arabidopsis_seedlings"><img alt="Research paper thumbnail of Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings" class="work-thumbnail" src="https://attachments.academia-assets.com/56681075/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737954/Isolation_and_identification_of_ubiquitin_related_proteins_from_Arabidopsis_seedlings">Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings</a></div><div class="wp-workCard_item"><span>Journal of Experimental Botany</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b9ff34eff7b6086384ccf7aaa303f93a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56681075,"asset_id":36737954,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56681075/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737954"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737954"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737954; 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Protein ubiquitination is one of the major posttranslational modifications occurring in eukaryotic cells. To obtain the proteomic dataset related to the ubiquitin (Ub)dependent regulatory system in Arabidopsis, affinity purification with an anti-Ub antibody under native condition was performed. Using MS/MS analysis, 196 distinct proteins represented by 251 distinct genes were identified. The identified proteins were involved in metabolism (23.0%), stress response (21.4%), translation (16.8%), transport (6.7%), cell morphology (3.6%), and signal transduction (1.5%), in addition to proteolysis (16.8%) to which proteasome subunits (14.3%) is included. On the basis of potential ubiquitination-targeting signal motifs, in-gel mobilities, and previous reports, 78 of the identified proteins were classified as ubiquitinated proteins and the rest were speculated to be associated proteins of ubiquitinated proteins. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737953"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737953/Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel"><img alt="Research paper thumbnail of Human simple renal cyst fluid contains a cyst formation-promoting activity for Madin-Darby canine kidney cells cultured in collagen gel" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737953/Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel">Human simple renal cyst fluid contains a cyst formation-promoting activity for Madin-Darby canine kidney cells cultured in collagen gel</a></div><div class="wp-workCard_item"><span>European Journal of Clinical Investigation</span><span>, 1996</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Simple renal cysts are commonly observed in the elderly, but few examinations have been performed...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Simple renal cysts are commonly observed in the elderly, but few examinations have been performed to study the mechanism of this disease. We have previously shown that simple renal cyst fluid contains some growth factors, including epidermal growth factor (EGF; less than 54 pg mL-1) and insulin-like growth factor I (IGF-I; 30-2070 pg mL-1) (M. Taide et al. Clin Chim Acta, 1993; 217:199-203). In this report, the biological significance of growth factors or of human simple renal cyst fluid on the formation of renal epithelial cyst was determined using Madin-Darby canine kidney (MDCK) cells cultured in collagen gel. Both EGF (200 pg mL-1 or more) and IGF-I (100 pg mL-1 or more) were found to stimulate cyst formation in MDCK cells. Additionally, 12/21 human simple renal cyst fluid samples were found to be capable of stimulating cyst formation in MDCK cells. This activity was due to a heat- and acid-labile protein and not inhibited by the anti-IGF-I neutralizing antibody. These results indicate that in some instances simple renal cyst fluid would thus appear to contain specific activity for cyst formation, which may affect cyst formation in vivo.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737953"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737953"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737953; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737953]").text(description); $(".js-view-count[data-work-id=36737953]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737953; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737953']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737953, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=36737953]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737953,"title":"Human simple renal cyst fluid contains a cyst formation-promoting activity for Madin-Darby canine kidney cells cultured in collagen gel","translated_title":"","metadata":{"abstract":"Simple renal cysts are commonly observed in the elderly, but few examinations have been performed to study the mechanism of this disease. We have previously shown that simple renal cyst fluid contains some growth factors, including epidermal growth factor (EGF; less than 54 pg mL-1) and insulin-like growth factor I (IGF-I; 30-2070 pg mL-1) (M. Taide et al. Clin Chim Acta, 1993; 217:199-203). In this report, the biological significance of growth factors or of human simple renal cyst fluid on the formation of renal epithelial cyst was determined using Madin-Darby canine kidney (MDCK) cells cultured in collagen gel. Both EGF (200 pg mL-1 or more) and IGF-I (100 pg mL-1 or more) were found to stimulate cyst formation in MDCK cells. Additionally, 12/21 human simple renal cyst fluid samples were found to be capable of stimulating cyst formation in MDCK cells. This activity was due to a heat- and acid-labile protein and not inhibited by the anti-IGF-I neutralizing antibody. These results indicate that in some instances simple renal cyst fluid would thus appear to contain specific activity for cyst formation, which may affect cyst formation in vivo.","publisher":"Wiley-Blackwell","publication_date":{"day":null,"month":null,"year":1996,"errors":{}},"publication_name":"European Journal of Clinical Investigation"},"translated_abstract":"Simple renal cysts are commonly observed in the elderly, but few examinations have been performed to study the mechanism of this disease. We have previously shown that simple renal cyst fluid contains some growth factors, including epidermal growth factor (EGF; less than 54 pg mL-1) and insulin-like growth factor I (IGF-I; 30-2070 pg mL-1) (M. Taide et al. Clin Chim Acta, 1993; 217:199-203). In this report, the biological significance of growth factors or of human simple renal cyst fluid on the formation of renal epithelial cyst was determined using Madin-Darby canine kidney (MDCK) cells cultured in collagen gel. Both EGF (200 pg mL-1 or more) and IGF-I (100 pg mL-1 or more) were found to stimulate cyst formation in MDCK cells. Additionally, 12/21 human simple renal cyst fluid samples were found to be capable of stimulating cyst formation in MDCK cells. This activity was due to a heat- and acid-labile protein and not inhibited by the anti-IGF-I neutralizing antibody. These results indicate that in some instances simple renal cyst fluid would thus appear to contain specific activity for cyst formation, which may affect cyst formation in vivo.","internal_url":"https://www.academia.edu/36737953/Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel","translated_internal_url":"","created_at":"2018-05-29T01:56:39.854-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[],"research_interests":[{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":329263,"name":"Epidermal Growth Factor","url":"https://www.academia.edu/Documents/in/Epidermal_Growth_Factor"},{"id":527900,"name":"Transforming Growth Factor Beta","url":"https://www.academia.edu/Documents/in/Transforming_Growth_Factor_Beta"},{"id":1127382,"name":"Hydrochloric Acid","url":"https://www.academia.edu/Documents/in/Hydrochloric_Acid"},{"id":1281442,"name":"Extracellular Space","url":"https://www.academia.edu/Documents/in/Extracellular_Space"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="22710268"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/22710268/Development_and_characterization_of_14_microsatellite_loci_in_the_beach_wolf_spider_Arctosa_littoralis_using_next_generation_sequencing"><img alt="Research paper thumbnail of Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/22710268/Development_and_characterization_of_14_microsatellite_loci_in_the_beach_wolf_spider_Arctosa_littoralis_using_next_generation_sequencing">Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/IreneBarriga">Irene Barriga</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MiguelFreitas11">Miguel Freitas</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TIgawa">Takeshi Igawa</a></span></div><div class="wp-workCard_item"><span>Conservation Genetics Resources</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based on 454 sequencing was used for obtain microsatellite loci for the wolf spiders. Fourteen polymorphic microsatellites were designed and tested as specific markers for the species. All markers were genotyped on 36 individuals from Las Playitas on the Baja California Peninsula. We observed low to medium genetic variation across most loci (with a mean of seven alleles per locus). Expected heterozygosity across all markers was considered intermediate (mean He = 0.57, range 0.05–0.91). These loci are the first to be characterized explicit for A. littoralis and should be useful for investigation of population structure and gene flow in this species.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="22710268"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="22710268"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 22710268; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=22710268]").text(description); $(".js-view-count[data-work-id=22710268]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 22710268; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='22710268']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 22710268, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=22710268]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":22710268,"title":"Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing","translated_title":"","metadata":{"abstract":"ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based on 454 sequencing was used for obtain microsatellite loci for the wolf spiders. Fourteen polymorphic microsatellites were designed and tested as specific markers for the species. All markers were genotyped on 36 individuals from Las Playitas on the Baja California Peninsula. We observed low to medium genetic variation across most loci (with a mean of seven alleles per locus). Expected heterozygosity across all markers was considered intermediate (mean He = 0.57, range 0.05–0.91). These loci are the first to be characterized explicit for A. littoralis and should be useful for investigation of population structure and gene flow in this species.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Conservation Genetics Resources"},"translated_abstract":"ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based on 454 sequencing was used for obtain microsatellite loci for the wolf spiders. Fourteen polymorphic microsatellites were designed and tested as specific markers for the species. All markers were genotyped on 36 individuals from Las Playitas on the Baja California Peninsula. We observed low to medium genetic variation across most loci (with a mean of seven alleles per locus). Expected heterozygosity across all markers was considered intermediate (mean He = 0.57, range 0.05–0.91). 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(Malvaceae) and intergeneric transferability</a></div><div class="wp-workCard_item"><span>Conservation Genetics Resources</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract We isolated and characterized ten microsatel-lite loci for Luehea divaricata, a South Am...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract We isolated and characterized ten microsatel-lite loci for Luehea divaricata, a South American out-crossing pioneer tree species that is frequently used in reforestation programs of tropical riparian forests in Brazil. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="5598153" id="papers"><div class="js-work-strip profile--work_container" data-work-id="36737964"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737964/Humanization_and_simultaneous_optimization_of_monoclonal_antibody"><img alt="Research paper thumbnail of Humanization and simultaneous optimization of monoclonal antibody" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737964/Humanization_and_simultaneous_optimization_of_monoclonal_antibody">Humanization and simultaneous optimization of monoclonal antibody</a></div><div class="wp-workCard_item"><span>Methods in molecular biology (Clifton, N.J.)</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Antibody humanization is an essential technology for reducing the potential risk of immunogenicit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Antibody humanization is an essential technology for reducing the potential risk of immunogenicity associated with animal-derived antibodies and has been applied to a majority of the therapeutic antibodies on the market. For developing an antibody molecule as a pharmaceutical at the current biotechnology level, however, other properties also have to be considered in parallel with humanization in antibody generation and optimization. This section describes the critical properties of therapeutic antibodies that should be sufficiently qualified, including immunogenicity, binding affinity, physiochemical stability, expression in host cells and pharmacokinetics, and the basic methodologies of antibody engineering involved. By simultaneously optimizing the antibody molecule in the light of these properties, it should prove possible to shorten the research and development period necessary to identify a highly qualified clinical candidate and consequently accelerate the start of the clinica...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737964"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737964"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737964; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737964]").text(description); $(".js-view-count[data-work-id=36737964]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737964; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737964']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737964, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=36737964]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737964,"title":"Humanization and simultaneous optimization of monoclonal antibody","translated_title":"","metadata":{"abstract":"Antibody humanization is an essential technology for reducing the potential risk of immunogenicity associated with animal-derived antibodies and has been applied to a majority of the therapeutic antibodies on the market. 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By simultaneously optimizing the antibody molecule in the light of these properties, it should prove possible to shorten the research and development period necessary to identify a highly qualified clinical candidate and consequently accelerate the start of the clinica...","internal_url":"https://www.academia.edu/36737964/Humanization_and_simultaneous_optimization_of_monoclonal_antibody","translated_internal_url":"","created_at":"2018-05-29T01:56:41.414-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Humanization_and_simultaneous_optimization_of_monoclonal_antibody","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[],"research_interests":[{"id":31264,"name":"Protein Engineering","url":"https://www.academia.edu/Documents/in/Protein_Engineering"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"urls":[]}, dispatcherData: dispatcherData }); 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window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737963]").text(description); $(".js-view-count[data-work-id=36737963]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737963; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737963']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737963, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "bfea9be1ad0641243dfd2d523fb19f46" } } $('.js-work-strip[data-work-id=36737963]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737963,"title":"MP-22.14: Prospective assessment of hot flashes during treatment with maximum androgen blockade using steroidal or non-steroidal antiandrogens for prostate cancer","translated_title":"","metadata":{"grobid_abstract":"Introduction: Androgen deprivation therapy for prostate cancer usually affects quality of life (QOL) in individual patients for long periods of time. Hot flashes, which are major adverse reactions to androgen deprivation therapy, can produce prolonged reductions in QOL. We recently conducted a 2-year longitudinal survey of the incidence, frequency and severity of hot flashes during maximum androgen blockade (MAB) therapy using steroidal or non-steroidal antiandrogens. Methods: A total of 151 patients with stage B-D prostate cancer, enrolled to this survey between May 2001 and June 2003, were randomized to receive MAB therapy using leuprorelin (LHRH agonist) and a steroidal antiandrogen, chlormadinone acetate (CMA group) or MAB using leuprorelin and a nonsteroidal antiandrogen, bicalutamide (BICA group). The incidence and frequency of, and distress due to hot flashes were evaluated with a self-entry questionnaire at baseline, and at 2-week intervals for the first 3 months, and then at 3-month intervals for 21 months. General and disease-specific QOL outcomes were measured with the Functional Assessment of Cancer Therapy (FACT) questionnaire at the same time. Results: Data were available for analysis on 124 patients. There was no difference in biochemical relapse-free survival at 2 years between CMA and BICA groups (88.0% vs. 84.4%, respectively). The incidence of hot flashes tended overall to be higher in BICA group than in CMA group. At 24 months, the incidence of flushing was 36.1% vs. 20.7%, and that of sweating was 33.3% vs. 21.4% in BICA and CMA groups, respectively. The frequency of hot flashes per day was higher in BICA Group than in CMA group (pϭ0.09 for flushing and pϭ0.021 for sweating). Patients in CMA group were significantly less likely to be distressed due to flushing (OR 0.47, pϽ0.001) and sweating (OR 0.61, pϭ0.01) than those in BICA","publication_date":{"day":null,"month":null,"year":2006,"errors":{}},"grobid_abstract_attachment_id":56681078},"translated_abstract":null,"internal_url":"https://www.academia.edu/36737963/MP_22_14_Prospective_assessment_of_hot_flashes_during_treatment_with_maximum_androgen_blockade_using_steroidal_or_non_steroidal_antiandrogens_for_prostate_cancer","translated_internal_url":"","created_at":"2018-05-29T01:56:41.282-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":56681078,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681078/thumbnails/1.jpg","file_name":"j.urology.2006.08.63620180529-20260-1sm44t2.pdf","download_url":"https://www.academia.edu/attachments/56681078/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"MP_22_14_Prospective_assessment_of_hot_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681078/j.urology.2006.08.63620180529-20260-1sm44t2-libre.pdf?1527584622=\u0026response-content-disposition=attachment%3B+filename%3DMP_22_14_Prospective_assessment_of_hot_f.pdf\u0026Expires=1732782714\u0026Signature=a9XsZkr0Xsw5lujJ9uLqYiIpVcQS-60avuTRiHotbNMkO7TbJwWBJijNz7jroSiw4N35YjmnMWOtMZ7DX~j35qpQbGnI~cnKyNMicIfMhaIoyg79W0Ndt-WeyKymuIa9QYrqyAwr2a1ajuFNBGiib55LR~zzS5u-H3ne4U-YkGkN3aZZkUuvUsG0dPjhZwS8Cod-amk-UniYKBnoMTndvf8LHw6cwJI6PhJ4ilYXi7~GYueoRtTmctoupm4cApqxVcDWcpuYN7dclChMdy~0T2hLWtIF4~Qu3fmzicMXiOxhZrXKBe8uZcg3B7qslys64t5aL3aGmT1MKYNtIlRIiQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"MP_22_14_Prospective_assessment_of_hot_flashes_during_treatment_with_maximum_androgen_blockade_using_steroidal_or_non_steroidal_antiandrogens_for_prostate_cancer","translated_slug":"","page_count":1,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[{"id":56681078,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681078/thumbnails/1.jpg","file_name":"j.urology.2006.08.63620180529-20260-1sm44t2.pdf","download_url":"https://www.academia.edu/attachments/56681078/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"MP_22_14_Prospective_assessment_of_hot_f.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681078/j.urology.2006.08.63620180529-20260-1sm44t2-libre.pdf?1527584622=\u0026response-content-disposition=attachment%3B+filename%3DMP_22_14_Prospective_assessment_of_hot_f.pdf\u0026Expires=1732782714\u0026Signature=a9XsZkr0Xsw5lujJ9uLqYiIpVcQS-60avuTRiHotbNMkO7TbJwWBJijNz7jroSiw4N35YjmnMWOtMZ7DX~j35qpQbGnI~cnKyNMicIfMhaIoyg79W0Ndt-WeyKymuIa9QYrqyAwr2a1ajuFNBGiib55LR~zzS5u-H3ne4U-YkGkN3aZZkUuvUsG0dPjhZwS8Cod-amk-UniYKBnoMTndvf8LHw6cwJI6PhJ4ilYXi7~GYueoRtTmctoupm4cApqxVcDWcpuYN7dclChMdy~0T2hLWtIF4~Qu3fmzicMXiOxhZrXKBe8uZcg3B7qslys64t5aL3aGmT1MKYNtIlRIiQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":653,"name":"Urology","url":"https://www.academia.edu/Documents/in/Urology"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"}],"urls":[]}, dispatcherData: dispatcherData }); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="25289246"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/25289246/Radiocesium_accumulation_in_the_anuran_frog_Rana_tagoi_tagoi_in_forest_ecosystems_after_the_Fukushima_Nuclear_Power_Plant_accident"><img alt="Research paper thumbnail of Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident" class="work-thumbnail" src="https://attachments.academia-assets.com/45590350/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/25289246/Radiocesium_accumulation_in_the_anuran_frog_Rana_tagoi_tagoi_in_forest_ecosystems_after_the_Fukushima_Nuclear_Power_Plant_accident">Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/ToshinoriOkuda">Toshinori Okuda</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TIgawa">Takeshi Igawa</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TeruhikoTakahara">Teruhiko Takahara</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://hiroshima-u.academia.edu/SatoruEndo">Satoru Endo</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/YurikaOba">Yurika Oba</a></span></div><div class="wp-workCard_item"><span>Environmental pollution (Barking, Essex : 1987)</span><span>, Jan 29, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Amphibians are key components in forest food webs. When examining radioactive contamination in an...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Amphibians are key components in forest food webs. When examining radioactive contamination in anurans, it is important to understand how radiocesium transfer occurs from lower to higher trophic levels in forest ecosystems. We investigated the activity concentration of radiocesium ((134)Cs and (137)Cs) in Tago&#39;s brown frog (Rana tagoi tagoi) captured on the forest floor approximately 2.5 years after the Fukushima Nuclear Power Plant (FNPP) accident. We collected 66 R. tagoi tagoi at different distances from the FNPP. Radiocesium accumulation showed positive correlations with the air radiation dose rate and litter contamination but not with distance from the FNPP. Whole-body radioactivity showed no correlation with body mass or length. Our results suggest that differences in the available food items result in large variability in individual contamination. Contamination level monitoring in terrestrial and aquatic amphibian is necessary for clarifying the processes and mechanisms o...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="619a780c7a1646a7c5122224010c7873" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":45590350,"asset_id":25289246,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/45590350/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="25289246"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="25289246"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 25289246; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=25289246]").text(description); $(".js-view-count[data-work-id=25289246]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 25289246; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='25289246']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 25289246, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "619a780c7a1646a7c5122224010c7873" } } $('.js-work-strip[data-work-id=25289246]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":25289246,"title":"Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident","translated_title":"","metadata":{"abstract":"Amphibians are key components in forest food webs. When examining radioactive contamination in anurans, it is important to understand how radiocesium transfer occurs from lower to higher trophic levels in forest ecosystems. We investigated the activity concentration of radiocesium ((134)Cs and (137)Cs) in Tago\u0026#39;s brown frog (Rana tagoi tagoi) captured on the forest floor approximately 2.5 years after the Fukushima Nuclear Power Plant (FNPP) accident. We collected 66 R. tagoi tagoi at different distances from the FNPP. Radiocesium accumulation showed positive correlations with the air radiation dose rate and litter contamination but not with distance from the FNPP. Whole-body radioactivity showed no correlation with body mass or length. Our results suggest that differences in the available food items result in large variability in individual contamination. Contamination level monitoring in terrestrial and aquatic amphibian is necessary for clarifying the processes and mechanisms o...","publication_date":{"day":29,"month":1,"year":2015,"errors":{}},"publication_name":"Environmental pollution (Barking, Essex : 1987)"},"translated_abstract":"Amphibians are key components in forest food webs. When examining radioactive contamination in anurans, it is important to understand how radiocesium transfer occurs from lower to higher trophic levels in forest ecosystems. We investigated the activity concentration of radiocesium ((134)Cs and (137)Cs) in Tago\u0026#39;s brown frog (Rana tagoi tagoi) captured on the forest floor approximately 2.5 years after the Fukushima Nuclear Power Plant (FNPP) accident. We collected 66 R. tagoi tagoi at different distances from the FNPP. Radiocesium accumulation showed positive correlations with the air radiation dose rate and litter contamination but not with distance from the FNPP. Whole-body radioactivity showed no correlation with body mass or length. Our results suggest that differences in the available food items result in large variability in individual contamination. 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="27451130"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/27451130/Genomic_structure_and_evolution_of_multigene_families_flowers_on_the_human_genome"><img alt="Research paper thumbnail of Genomic structure and evolution of multigene families: "flowers" on the human genome" class="work-thumbnail" src="https://attachments.academia-assets.com/47710212/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/27451130/Genomic_structure_and_evolution_of_multigene_families_flowers_on_the_human_genome">Genomic structure and evolution of multigene families: "flowers" on the human genome</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://nanyang.academia.edu/HieLimKim">Hie Lim Kim</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TIgawa">Takeshi Igawa</a></span></div><div class="wp-workCard_item"><span>International journal of evolutionary biology</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="c9f4e524d47b9279ab97b3644aad7137" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":47710212,"asset_id":27451130,"asset_type":"Work","button_location":"profile"}" 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adjacent chromosomal regions. We named such structures \"Flowers\" because the pattern observed on dot plots resembles a flower. We detected a total of 291 Flowers in the human genome. They were predominantly located in euchromatic regions. Flowers are gene-rich compared to the average gene density of the genome. Genes involved in systems receiving environmental information, such as immunity and detoxification, were overrepresented in Flowers. Within a Flower, the mean number of duplication units was approximately four. The maximum and minimum identities between homologs in a Flower showed different distributions; the maximum identity was often concentrated to 100% identity, while the minimum identity was evenly distributed in the range of 78% to 100%. Using a gene conversion detection test, we found frequent and/or recent gene conversion events within the tested Flowers. Interestingly, many of those converted regions contained protein-coding genes. Computer simulation studies suggest that one role of such frequent gene conversions is the elongation of the life span of gene families in a Flower by the resurrection of pseudogenes.","publication_date":{"day":null,"month":null,"year":2012,"errors":{}},"publication_name":"International journal of evolutionary biology","grobid_abstract_attachment_id":47710212},"translated_abstract":null,"internal_url":"https://www.academia.edu/27451130/Genomic_structure_and_evolution_of_multigene_families_flowers_on_the_human_genome","translated_internal_url":"","created_at":"2016-08-01T19:00:31.205-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":34029428,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[{"id":22992649,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":51641042,"co_author_invite_id":1020839,"email":"t***a@hiroshima-u.ac.jp","display_order":0,"name":"Takeshi Igawa","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992651,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":null,"co_author_invite_id":5129975,"email":"i***o@soken.ac.jp","display_order":4194304,"name":"Mineyo Iwase","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992653,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":null,"co_author_invite_id":5129976,"email":"k***o@soken.ac.jp","display_order":6291456,"name":"Satoko Kaneko","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992659,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":36880064,"co_author_invite_id":null,"email":"s***a@soken.ac.jp","display_order":7340032,"name":"Yoko Satta","title":"Genomic structure and evolution of multigene families: \"flowers\" on the human genome"},{"id":22992662,"work_id":27451130,"tagging_user_id":34029428,"tagged_user_id":38302016,"co_author_invite_id":null,"email":"t***a@soken.ac.jp","display_order":7864320,"name":"N. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737958"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737958/Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs"><img alt="Research paper thumbnail of Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737958/Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs">Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs</a></div><div class="wp-workCard_item"><span>Journal of Heredity</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis, Odorrana supranarina, and Odorrana utsunomiyaorum, belong to the family Ranidae and are endemically distributed in Okinawa (O. narina), Amami and Tokunoshima (O. amamiensis), and Ishigaki and Iriomote (O. supranarina and O. utsunomiyaorum) Islands. Because of varying distribution patterns, this species complex is an intrinsic model for speciation and adaptation. For effective conservation and molecular ecological studies, further genetic information is needed. For rapid, cost-effective development of several microsatellite markers for these and 2 other species, we used next-generation sequencing technology of Ion Torrent PGM™. Distribution patterns of repeat motifs of microsatellite loci in these modern frog species (Neobatrachia) were similarly skewed. We isolated and characterized 20 new microsatellite loci of O. narina and validated cross-amplification in the three-related species. Seventeen, 16, and 13 loci were cross-amplified in O. amamiensis, O. supranarina, and O. utsunomiyaorum, respectively, reflecting close genetic relationships between them. Mean number of alleles and expected heterozygosity of newly isolated loci varied depending on the size of each inhabited island. Our findings suggested the suitability of Ion Torrent PGM™ for microsatellite marker development. The new markers developed for the O. narina complex will be applicable in conservation genetics and molecular ecological studies.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737958"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737958"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737958; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737958]").text(description); $(".js-view-count[data-work-id=36737958]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737958; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737958']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737958, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=36737958]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737958,"title":"Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs","translated_title":"","metadata":{"abstract":"The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis, Odorrana supranarina, and Odorrana utsunomiyaorum, belong to the family Ranidae and are endemically distributed in Okinawa (O. narina), Amami and Tokunoshima (O. amamiensis), and Ishigaki and Iriomote (O. supranarina and O. utsunomiyaorum) Islands. Because of varying distribution patterns, this species complex is an intrinsic model for speciation and adaptation. For effective conservation and molecular ecological studies, further genetic information is needed. For rapid, cost-effective development of several microsatellite markers for these and 2 other species, we used next-generation sequencing technology of Ion Torrent PGM™. Distribution patterns of repeat motifs of microsatellite loci in these modern frog species (Neobatrachia) were similarly skewed. We isolated and characterized 20 new microsatellite loci of O. narina and validated cross-amplification in the three-related species. Seventeen, 16, and 13 loci were cross-amplified in O. amamiensis, O. supranarina, and O. utsunomiyaorum, respectively, reflecting close genetic relationships between them. Mean number of alleles and expected heterozygosity of newly isolated loci varied depending on the size of each inhabited island. Our findings suggested the suitability of Ion Torrent PGM™ for microsatellite marker development. The new markers developed for the O. narina complex will be applicable in conservation genetics and molecular ecological studies.","publication_date":{"day":null,"month":null,"year":2014,"errors":{}},"publication_name":"Journal of Heredity"},"translated_abstract":"The endangered Ryukyu tip-nosed frog Odorrana narina and its related species, Odorrana amamiensis, Odorrana supranarina, and Odorrana utsunomiyaorum, belong to the family Ranidae and are endemically distributed in Okinawa (O. narina), Amami and Tokunoshima (O. amamiensis), and Ishigaki and Iriomote (O. supranarina and O. utsunomiyaorum) Islands. Because of varying distribution patterns, this species complex is an intrinsic model for speciation and adaptation. For effective conservation and molecular ecological studies, further genetic information is needed. For rapid, cost-effective development of several microsatellite markers for these and 2 other species, we used next-generation sequencing technology of Ion Torrent PGM™. Distribution patterns of repeat motifs of microsatellite loci in these modern frog species (Neobatrachia) were similarly skewed. We isolated and characterized 20 new microsatellite loci of O. narina and validated cross-amplification in the three-related species. Seventeen, 16, and 13 loci were cross-amplified in O. amamiensis, O. supranarina, and O. utsunomiyaorum, respectively, reflecting close genetic relationships between them. Mean number of alleles and expected heterozygosity of newly isolated loci varied depending on the size of each inhabited island. Our findings suggested the suitability of Ion Torrent PGM™ for microsatellite marker development. The new markers developed for the O. narina complex will be applicable in conservation genetics and molecular ecological studies.","internal_url":"https://www.academia.edu/36737958/Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs","translated_internal_url":"","created_at":"2018-05-29T01:56:40.370-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Microsatellite_Marker_Development_by_Multiplex_Ion_Torrent_PGM_Sequencing_a_Case_Study_of_the_Endangered_Odorrana_narina_Complex_of_Frogs","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[],"research_interests":[{"id":156,"name":"Genetics","url":"https://www.academia.edu/Documents/in/Genetics"},{"id":17158,"name":"Japan","url":"https://www.academia.edu/Documents/in/Japan"},{"id":23979,"name":"Endangered Species","url":"https://www.academia.edu/Documents/in/Endangered_Species"},{"id":42324,"name":"Heredity","url":"https://www.academia.edu/Documents/in/Heredity"},{"id":372410,"name":"Genotype","url":"https://www.academia.edu/Documents/in/Genotype"},{"id":426361,"name":"Ranidae","url":"https://www.academia.edu/Documents/in/Ranidae"},{"id":569436,"name":"Genomic Library","url":"https://www.academia.edu/Documents/in/Genomic_Library"},{"id":719974,"name":"Conservation of Natural Resources","url":"https://www.academia.edu/Documents/in/Conservation_of_Natural_Resources"},{"id":784076,"name":"Species Specificity","url":"https://www.academia.edu/Documents/in/Species_Specificity"},{"id":1232430,"name":"Genetic Markers","url":"https://www.academia.edu/Documents/in/Genetic_Markers"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737957"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737957/Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments"><img alt="Research paper thumbnail of Evolutionary Relationships and Reproductive Isolating Mechanisms in the Rice Frog (Fejervarya limnocharis) Species Complex from Sri Lanka, Thailand, Taiwan and Japan, Inferred from mtDNA Gene Sequences, Allozymes, and Crossing Experiments" class="work-thumbnail" src="https://attachments.academia-assets.com/56681077/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737957/Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments">Evolutionary Relationships and Reproductive Isolating Mechanisms in the Rice Frog (Fejervarya limnocharis) Species Complex from Sri Lanka, Thailand, Taiwan and Japan, Inferred from mtDNA Gene Sequences, Allozymes, and Crossing Experiments</a></div><div class="wp-workCard_item"><span>Zoological Science</span><span>, 2007</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="429d92b433584e7cfabc06b67c382174" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56681077,"asset_id":36737957,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56681077/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737957"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737957"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737957; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "429d92b433584e7cfabc06b67c382174" } } $('.js-work-strip[data-work-id=36737957]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737957,"title":"Evolutionary Relationships and Reproductive Isolating Mechanisms in the Rice Frog (Fejervarya limnocharis) Species Complex from Sri Lanka, Thailand, Taiwan and Japan, Inferred from mtDNA Gene Sequences, Allozymes, and Crossing Experiments","translated_title":"","metadata":{"publisher":"Zoological Society of Japan","grobid_abstract":"The rice frog (Fejervarya limnocharis) species complex is widely distributed, from India to Japan, and most prevalently in Southeast Asia. Conspicuous morphological variation has been reported for this species complex throughout its distribution range. In the present study, we used mtDNA gene sequence and allozyme analyses to infer evolutionary affinities within this species complex using eight populations (Sri Lanka; Bangkok and Ranong in Thailand; Taiwan; and Hiroshima, Okinawa, Ishigaki and Iriomote in Japan). We also conducted crossing experiments among four populations from Japan, Thailand, and Sri Lanka in order to find out more about the reproductive isolating mechanisms that might exist among the East, Southeast, and South Asian populations of this species complex. The crossing experiments revealed that the Sri Lanka population is reproductively isolated from the Hiroshima, Bangkok, and Ranong populations by complete hybrid inviability, and that the Bangkok population may be reproductively isolated from the Hiroshima population by partial hybrid inviability. Thus, it is not unreasonable to regard the Sri Lanka population as a species separated from F. limnocharis. The mtDNA and allozyme data showed that the Ranong population is most closely related to the Bangkok population in nuclear genome, but more similar to the Okinawa and Taiwan populations in mtDNA genome. The present, preliminary survey may raise questions about the species status of these particular populations and also about the nature of the biological species concept.","publication_date":{"day":null,"month":null,"year":2007,"errors":{}},"publication_name":"Zoological Science","grobid_abstract_attachment_id":56681077},"translated_abstract":null,"internal_url":"https://www.academia.edu/36737957/Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments","translated_internal_url":"","created_at":"2018-05-29T01:56:40.267-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":56681077,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681077/thumbnails/1.jpg","file_name":"0fcfd51205a10d7dd1000000.pdf","download_url":"https://www.academia.edu/attachments/56681077/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Evolutionary_Relationships_and_Reproduct.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681077/0fcfd51205a10d7dd1000000.pdf?1527584266=\u0026response-content-disposition=attachment%3B+filename%3DEvolutionary_Relationships_and_Reproduct.pdf\u0026Expires=1732782714\u0026Signature=A0STWAUYbRDjx5i~fzL36jZNYA-uASwXp-hVMdac4AMGDdm5dFLpnWOAQsOKD0trlMSQdHIADHbbsv1jVkqm0-irH7orTznDInrEVQsSa4TmV1tv4NrnNoypvOTNf4Alr1oYX-i61GSQY1ZZVzSFTDipKIP7wDrv6TRfM78RdXuQS843FP5FbwLrwTj5m~EKz8Eg6EJRDzbRLnuRKNKmK~Cx2cVrPGKCf~CZq3chGNkzEH2jb9uUoATZ4uxj75T9V02NljV-aee7~LWBuwbK8xiEXqw-MY35U4-bWOU7nyBPbvgJbJxo3FWaEHJ2AcxKwDT4Df-tk12z9j6iQJ3gXw__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Evolutionary_Relationships_and_Reproductive_Isolating_Mechanisms_in_the_Rice_Frog_Fejervarya_limnocharis_Species_Complex_from_Sri_Lanka_Thailand_Taiwan_and_Japan_Inferred_from_mtDNA_Gene_Sequences_Allozymes_and_Crossing_Experiments","translated_slug":"","page_count":17,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi 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Evolution","url":"https://www.academia.edu/Documents/in/Molecular_Evolution"},{"id":9850,"name":"Sri Lanka","url":"https://www.academia.edu/Documents/in/Sri_Lanka"},{"id":10096,"name":"Thailand","url":"https://www.academia.edu/Documents/in/Thailand"},{"id":17158,"name":"Japan","url":"https://www.academia.edu/Documents/in/Japan"},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny"},{"id":55163,"name":"Enzymes","url":"https://www.academia.edu/Documents/in/Enzymes"},{"id":63093,"name":"Mitochondrial DNA","url":"https://www.academia.edu/Documents/in/Mitochondrial_DNA"},{"id":67484,"name":"Sequence alignment","url":"https://www.academia.edu/Documents/in/Sequence_alignment"},{"id":86952,"name":"Haplotypes","url":"https://www.academia.edu/Documents/in/Haplotypes"},{"id":94451,"name":"Taiwan","url":"https://www.academia.edu/Documents/in/Taiwan"},{"id":358670,"name":"Reproductive 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href="https://www.academia.edu/36737956/An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan"><img alt="Research paper thumbnail of An Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan" class="work-thumbnail" src="https://attachments.academia-assets.com/56681079/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737956/An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan">An Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan</a></div><div class="wp-workCard_item"><span>Animals</span><span>, 2013</span></div><div class="wp-workCard_item 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This is the first published report of successfully propagating an endangered species by using breeding cages in a laboratory setting for captive breeding. Our findings on the natural breeding and raising of larvae and adults are useful in breeding this endangered species, and can be applied to the preservation of other similarly wild and endangered species.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Animals","grobid_abstract_attachment_id":56681079},"translated_abstract":null,"internal_url":"https://www.academia.edu/36737956/An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan","translated_internal_url":"","created_at":"2018-05-29T01:56:40.173-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":56681079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681079/thumbnails/1.jpg","file_name":"animals-03-00680.pdf","download_url":"https://www.academia.edu/attachments/56681079/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"An_Attempt_at_Captive_Breeding_of_the_En.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681079/animals-03-00680-libre.pdf?1527584624=\u0026response-content-disposition=attachment%3B+filename%3DAn_Attempt_at_Captive_Breeding_of_the_En.pdf\u0026Expires=1732782714\u0026Signature=S8KSezzM1bT9a-Yz3iTkYA5jyBN1KVwKySNk4i8xbv4H2Qfojqf3Ll7Xx0m3zmghsCv-loLxjyu0jYvk1U5Ho5-nV6hCY2yLldY46WKuGBlih36qs~q2liVAWvg5h1NSxXpNUtuILYnY0hqBtD-u3FH29EEo6TsSgP7XFPqj9kyGlDr1ixmEcP-036NvVWaCzikY8VoaU84~bvMl7nJA~ULIwx8y0P9a~7mnXoIr0TSWRwWlx5izD6Q0p3DBpYbHgL4FMrqD4pq47vi~PHx3sKQHEwt6yTIznRK3x0uongguhVXDjOzqCHXiu9krv4ao0CcQzsqRgaWcqtX01wkX~A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"An_Attempt_at_Captive_Breeding_of_the_Endangered_Newt_Echinotriton_andersoni_from_the_Central_Ryukyus_in_Japan","translated_slug":"","page_count":13,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[{"id":56681079,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681079/thumbnails/1.jpg","file_name":"animals-03-00680.pdf","download_url":"https://www.academia.edu/attachments/56681079/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNCw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"An_Attempt_at_Captive_Breeding_of_the_En.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681079/animals-03-00680-libre.pdf?1527584624=\u0026response-content-disposition=attachment%3B+filename%3DAn_Attempt_at_Captive_Breeding_of_the_En.pdf\u0026Expires=1732782714\u0026Signature=S8KSezzM1bT9a-Yz3iTkYA5jyBN1KVwKySNk4i8xbv4H2Qfojqf3Ll7Xx0m3zmghsCv-loLxjyu0jYvk1U5Ho5-nV6hCY2yLldY46WKuGBlih36qs~q2liVAWvg5h1NSxXpNUtuILYnY0hqBtD-u3FH29EEo6TsSgP7XFPqj9kyGlDr1ixmEcP-036NvVWaCzikY8VoaU84~bvMl7nJA~ULIwx8y0P9a~7mnXoIr0TSWRwWlx5izD6Q0p3DBpYbHgL4FMrqD4pq47vi~PHx3sKQHEwt6yTIznRK3x0uongguhVXDjOzqCHXiu9krv4ao0CcQzsqRgaWcqtX01wkX~A__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":200081,"name":"IUCN Red List","url":"https://www.academia.edu/Documents/in/IUCN_Red_List"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737955"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737955/Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria"><img alt="Research paper thumbnail of Isolation and characterization of the plant glsA promoter from Alstroemeria" class="work-thumbnail" src="https://attachments.academia-assets.com/56681080/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737955/Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria">Isolation and characterization of the plant glsA promoter from Alstroemeria</a></div><div class="wp-workCard_item"><span>Plant Biology</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1dd6424661ce6a2976ab13e2baf9798e" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56681080,"asset_id":36737955,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56681080/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737955"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737955"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737955; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "1dd6424661ce6a2976ab13e2baf9798e" } } $('.js-work-strip[data-work-id=36737955]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737955,"title":"Isolation and characterization of the plant glsA promoter from Alstroemeria","translated_title":"","metadata":{"publisher":"Wiley-Blackwell","grobid_abstract":"The differentiation of a vegetative cell and a generative cell is a critical event during pollen development. The Lilium GlsA is known to localize in pollen and is considered to be involved in development of the generative cell. Here, we cloned a glsA ortholog from Alstroemeria, a commercially important cut flower. The expression of AaglsA (Alstroemeria aurea glsA) transcripts increased gradually after pollen mitosis I (PMI) and reached a significant level when the generative cell started to elongate. Analysis of the promoter of AaglsA suggests that AaglsA expression is controlled by several cis-regulatory elements during pollen development. This is the first investigation of reproductive factors regulating male gametogenesis in Alstroemeria.","publication_date":{"day":null,"month":null,"year":2009,"errors":{}},"publication_name":"Plant Biology","grobid_abstract_attachment_id":56681080},"translated_abstract":null,"internal_url":"https://www.academia.edu/36737955/Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria","translated_internal_url":"","created_at":"2018-05-29T01:56:40.080-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":56681080,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681080/thumbnails/1.jpg","file_name":"j.1438-8677.2008.00177.x20180529-20264-7q96lv.pdf","download_url":"https://www.academia.edu/attachments/56681080/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Isolation_and_characterization_of_the_pl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681080/j.1438-8677.2008.00177.x20180529-20264-7q96lv-libre.pdf?1527584627=\u0026response-content-disposition=attachment%3B+filename%3DIsolation_and_characterization_of_the_pl.pdf\u0026Expires=1732782715\u0026Signature=Hbd4tpxojqZby680btD5IFeoV6K8P1ZFQc3-RwZ9-DdLR1cwXHQXUNplqW~dLdpMzZytxwkZqqhyiRiv4X-61Y5Sw2CPNNMAUOcyBQzvIWom3SdwShZqsU3WbNWrXXVNqOdtg5LWI067Uimh40p7j3QPhgEV4zmYoWz9IsPvbX74NPVi968j3rpvMgnUuZwCgFy22MXI6GuO7BVdFXaGr8Ty59VCNUU4bVNxHdr-Bvb3rSiUH-VwYySZSB-Y9HJIOn~GlDQEJtYJANgLL~7H7pwKgf-sClBj~fmtahuwdUq3gMf~2wV0qtxTPdEYlmMOcStv9bwe-Z5Lo~UT8b0~ew__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Isolation_and_characterization_of_the_plant_glsA_promoter_from_Alstroemeria","translated_slug":"","page_count":8,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[{"id":56681080,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/56681080/thumbnails/1.jpg","file_name":"j.1438-8677.2008.00177.x20180529-20264-7q96lv.pdf","download_url":"https://www.academia.edu/attachments/56681080/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Isolation_and_characterization_of_the_pl.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/56681080/j.1438-8677.2008.00177.x20180529-20264-7q96lv-libre.pdf?1527584627=\u0026response-content-disposition=attachment%3B+filename%3DIsolation_and_characterization_of_the_pl.pdf\u0026Expires=1732782715\u0026Signature=Hbd4tpxojqZby680btD5IFeoV6K8P1ZFQc3-RwZ9-DdLR1cwXHQXUNplqW~dLdpMzZytxwkZqqhyiRiv4X-61Y5Sw2CPNNMAUOcyBQzvIWom3SdwShZqsU3WbNWrXXVNqOdtg5LWI067Uimh40p7j3QPhgEV4zmYoWz9IsPvbX74NPVi968j3rpvMgnUuZwCgFy22MXI6GuO7BVdFXaGr8Ty59VCNUU4bVNxHdr-Bvb3rSiUH-VwYySZSB-Y9HJIOn~GlDQEJtYJANgLL~7H7pwKgf-sClBj~fmtahuwdUq3gMf~2wV0qtxTPdEYlmMOcStv9bwe-Z5Lo~UT8b0~ew__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology"},{"id":55267,"name":"Mitosis","url":"https://www.academia.edu/Documents/in/Mitosis"},{"id":83086,"name":"Pollen","url":"https://www.academia.edu/Documents/in/Pollen"},{"id":121673,"name":"Promoter","url":"https://www.academia.edu/Documents/in/Promoter"},{"id":295728,"name":"Molecular cloning","url":"https://www.academia.edu/Documents/in/Molecular_cloning"},{"id":305219,"name":"Alstroemeria","url":"https://www.academia.edu/Documents/in/Alstroemeria"},{"id":809882,"name":"Base Sequence","url":"https://www.academia.edu/Documents/in/Base_Sequence"},{"id":1181939,"name":"PLANT PROTEINS","url":"https://www.academia.edu/Documents/in/PLANT_PROTEINS"},{"id":2467566,"name":"Molecular Sequence Data","url":"https://www.academia.edu/Documents/in/Molecular_Sequence_Data"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737954"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737954/Isolation_and_identification_of_ubiquitin_related_proteins_from_Arabidopsis_seedlings"><img alt="Research paper thumbnail of Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings" class="work-thumbnail" src="https://attachments.academia-assets.com/56681075/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737954/Isolation_and_identification_of_ubiquitin_related_proteins_from_Arabidopsis_seedlings">Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings</a></div><div class="wp-workCard_item"><span>Journal of Experimental Botany</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b9ff34eff7b6086384ccf7aaa303f93a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":56681075,"asset_id":36737954,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/56681075/download_file?st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&st=MTczMjc3OTExNSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737954"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737954"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737954; 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Protein ubiquitination is one of the major posttranslational modifications occurring in eukaryotic cells. To obtain the proteomic dataset related to the ubiquitin (Ub)dependent regulatory system in Arabidopsis, affinity purification with an anti-Ub antibody under native condition was performed. Using MS/MS analysis, 196 distinct proteins represented by 251 distinct genes were identified. The identified proteins were involved in metabolism (23.0%), stress response (21.4%), translation (16.8%), transport (6.7%), cell morphology (3.6%), and signal transduction (1.5%), in addition to proteolysis (16.8%) to which proteasome subunits (14.3%) is included. On the basis of potential ubiquitination-targeting signal motifs, in-gel mobilities, and previous reports, 78 of the identified proteins were classified as ubiquitinated proteins and the rest were speculated to be associated proteins of ubiquitinated proteins. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="36737953"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/36737953/Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel"><img alt="Research paper thumbnail of Human simple renal cyst fluid contains a cyst formation-promoting activity for Madin-Darby canine kidney cells cultured in collagen gel" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/36737953/Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel">Human simple renal cyst fluid contains a cyst formation-promoting activity for Madin-Darby canine kidney cells cultured in collagen gel</a></div><div class="wp-workCard_item"><span>European Journal of Clinical Investigation</span><span>, 1996</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Simple renal cysts are commonly observed in the elderly, but few examinations have been performed...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Simple renal cysts are commonly observed in the elderly, but few examinations have been performed to study the mechanism of this disease. We have previously shown that simple renal cyst fluid contains some growth factors, including epidermal growth factor (EGF; less than 54 pg mL-1) and insulin-like growth factor I (IGF-I; 30-2070 pg mL-1) (M. Taide et al. Clin Chim Acta, 1993; 217:199-203). In this report, the biological significance of growth factors or of human simple renal cyst fluid on the formation of renal epithelial cyst was determined using Madin-Darby canine kidney (MDCK) cells cultured in collagen gel. Both EGF (200 pg mL-1 or more) and IGF-I (100 pg mL-1 or more) were found to stimulate cyst formation in MDCK cells. Additionally, 12/21 human simple renal cyst fluid samples were found to be capable of stimulating cyst formation in MDCK cells. This activity was due to a heat- and acid-labile protein and not inhibited by the anti-IGF-I neutralizing antibody. These results indicate that in some instances simple renal cyst fluid would thus appear to contain specific activity for cyst formation, which may affect cyst formation in vivo.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="36737953"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="36737953"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 36737953; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=36737953]").text(description); $(".js-view-count[data-work-id=36737953]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 36737953; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='36737953']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 36737953, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=36737953]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":36737953,"title":"Human simple renal cyst fluid contains a cyst formation-promoting activity for Madin-Darby canine kidney cells cultured in collagen gel","translated_title":"","metadata":{"abstract":"Simple renal cysts are commonly observed in the elderly, but few examinations have been performed to study the mechanism of this disease. We have previously shown that simple renal cyst fluid contains some growth factors, including epidermal growth factor (EGF; less than 54 pg mL-1) and insulin-like growth factor I (IGF-I; 30-2070 pg mL-1) (M. Taide et al. Clin Chim Acta, 1993; 217:199-203). In this report, the biological significance of growth factors or of human simple renal cyst fluid on the formation of renal epithelial cyst was determined using Madin-Darby canine kidney (MDCK) cells cultured in collagen gel. Both EGF (200 pg mL-1 or more) and IGF-I (100 pg mL-1 or more) were found to stimulate cyst formation in MDCK cells. Additionally, 12/21 human simple renal cyst fluid samples were found to be capable of stimulating cyst formation in MDCK cells. This activity was due to a heat- and acid-labile protein and not inhibited by the anti-IGF-I neutralizing antibody. These results indicate that in some instances simple renal cyst fluid would thus appear to contain specific activity for cyst formation, which may affect cyst formation in vivo.","publisher":"Wiley-Blackwell","publication_date":{"day":null,"month":null,"year":1996,"errors":{}},"publication_name":"European Journal of Clinical Investigation"},"translated_abstract":"Simple renal cysts are commonly observed in the elderly, but few examinations have been performed to study the mechanism of this disease. We have previously shown that simple renal cyst fluid contains some growth factors, including epidermal growth factor (EGF; less than 54 pg mL-1) and insulin-like growth factor I (IGF-I; 30-2070 pg mL-1) (M. Taide et al. Clin Chim Acta, 1993; 217:199-203). In this report, the biological significance of growth factors or of human simple renal cyst fluid on the formation of renal epithelial cyst was determined using Madin-Darby canine kidney (MDCK) cells cultured in collagen gel. Both EGF (200 pg mL-1 or more) and IGF-I (100 pg mL-1 or more) were found to stimulate cyst formation in MDCK cells. Additionally, 12/21 human simple renal cyst fluid samples were found to be capable of stimulating cyst formation in MDCK cells. This activity was due to a heat- and acid-labile protein and not inhibited by the anti-IGF-I neutralizing antibody. These results indicate that in some instances simple renal cyst fluid would thus appear to contain specific activity for cyst formation, which may affect cyst formation in vivo.","internal_url":"https://www.academia.edu/36737953/Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel","translated_internal_url":"","created_at":"2018-05-29T01:56:39.854-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":51641042,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[],"slug":"Human_simple_renal_cyst_fluid_contains_a_cyst_formation_promoting_activity_for_Madin_Darby_canine_kidney_cells_cultured_in_collagen_gel","translated_slug":"","page_count":null,"language":"en","content_type":"Work","owner":{"id":51641042,"first_name":"Takeshi","middle_initials":null,"last_name":"Igawa","page_name":"TIgawa","domain_name":"independent","created_at":"2016-08-03T00:02:00.341-07:00","display_name":"Takeshi Igawa","url":"https://independent.academia.edu/TIgawa"},"attachments":[],"research_interests":[{"id":37782,"name":"Cell Culture","url":"https://www.academia.edu/Documents/in/Cell_Culture"},{"id":52438,"name":"Dogs","url":"https://www.academia.edu/Documents/in/Dogs"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences"},{"id":329263,"name":"Epidermal Growth Factor","url":"https://www.academia.edu/Documents/in/Epidermal_Growth_Factor"},{"id":527900,"name":"Transforming Growth Factor Beta","url":"https://www.academia.edu/Documents/in/Transforming_Growth_Factor_Beta"},{"id":1127382,"name":"Hydrochloric Acid","url":"https://www.academia.edu/Documents/in/Hydrochloric_Acid"},{"id":1281442,"name":"Extracellular Space","url":"https://www.academia.edu/Documents/in/Extracellular_Space"}],"urls":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="22710268"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/22710268/Development_and_characterization_of_14_microsatellite_loci_in_the_beach_wolf_spider_Arctosa_littoralis_using_next_generation_sequencing"><img alt="Research paper thumbnail of Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/22710268/Development_and_characterization_of_14_microsatellite_loci_in_the_beach_wolf_spider_Arctosa_littoralis_using_next_generation_sequencing">Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/IreneBarriga">Irene Barriga</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MiguelFreitas11">Miguel Freitas</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/TIgawa">Takeshi Igawa</a></span></div><div class="wp-workCard_item"><span>Conservation Genetics Resources</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based on 454 sequencing was used for obtain microsatellite loci for the wolf spiders. Fourteen polymorphic microsatellites were designed and tested as specific markers for the species. All markers were genotyped on 36 individuals from Las Playitas on the Baja California Peninsula. We observed low to medium genetic variation across most loci (with a mean of seven alleles per locus). Expected heterozygosity across all markers was considered intermediate (mean He = 0.57, range 0.05–0.91). These loci are the first to be characterized explicit for A. littoralis and should be useful for investigation of population structure and gene flow in this species.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="22710268"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="22710268"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 22710268; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=22710268]").text(description); $(".js-view-count[data-work-id=22710268]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 22710268; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='22710268']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 22710268, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=22710268]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":22710268,"title":"Development and characterization of 14 microsatellite loci in the beach wolf spider (Arctosa littoralis), using next-generation sequencing","translated_title":"","metadata":{"abstract":"ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based on 454 sequencing was used for obtain microsatellite loci for the wolf spiders. Fourteen polymorphic microsatellites were designed and tested as specific markers for the species. All markers were genotyped on 36 individuals from Las Playitas on the Baja California Peninsula. We observed low to medium genetic variation across most loci (with a mean of seven alleles per locus). Expected heterozygosity across all markers was considered intermediate (mean He = 0.57, range 0.05–0.91). These loci are the first to be characterized explicit for A. littoralis and should be useful for investigation of population structure and gene flow in this species.","publication_date":{"day":null,"month":null,"year":2013,"errors":{}},"publication_name":"Conservation Genetics Resources"},"translated_abstract":"ABSTRACT The beach wolf spider, Arctosa littoralis, inhabits areas near water. A technique based on 454 sequencing was used for obtain microsatellite loci for the wolf spiders. Fourteen polymorphic microsatellites were designed and tested as specific markers for the species. All markers were genotyped on 36 individuals from Las Playitas on the Baja California Peninsula. We observed low to medium genetic variation across most loci (with a mean of seven alleles per locus). Expected heterozygosity across all markers was considered intermediate (mean He = 0.57, range 0.05–0.91). 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(Malvaceae) and intergeneric transferability</a></div><div class="wp-workCard_item"><span>Conservation Genetics Resources</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Abstract We isolated and characterized ten microsatel-lite loci for Luehea divaricata, a South Am...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Abstract We isolated and characterized ten microsatel-lite loci for Luehea divaricata, a South American out-crossing pioneer tree species that is frequently used in reforestation programs of tropical riparian forests in Brazil. 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