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between T cells and trafficking of HIV1</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A wide variety of cell types, including immune cells, have been observed to frequently interact via transient, long-distance membrane connections [1–17]. However, considerable heterogeneity in their structure, mode of formation and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_51869891" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A wide variety of cell types, including immune cells, have been observed to frequently interact via transient, long-distance membrane connections [1–17]. However, considerable heterogeneity in their structure, mode of formation and functional properties has emerged, suggesting the existence of distinct subclasses [18–21]. Open-ended tunneling nanotubes allow for the trafficking of cytoplasmic material, e.g. endocytic vesicles, or the transmission of calcium</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/51869891" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="225e39d0a7de6750f641d69c21242370" rel="nofollow" data-download="{"attachment_id":69398595,"asset_id":51869891,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/69398595/download_file?st=MTczMjc4NTE1Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="103758298" href="https://independent.academia.edu/JAlakoskela">Juha-Matti Alakoskela</a><script data-card-contents-for-user="103758298" type="text/json">{"id":103758298,"first_name":"Juha-Matti","last_name":"Alakoskela","domain_name":"independent","page_name":"JAlakoskela","display_name":"Juha-Matti Alakoskela","profile_url":"https://independent.academia.edu/JAlakoskela?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_51869891 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="51869891"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 51869891, container: ".js-paper-rank-work_51869891", }); 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href="https://www.academia.edu/36103397/Phorbol_esters_induce_translocation_of_the_nPKC_p105_to_membrane_in_mussel_hemocytes">Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_36103397" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/36103397" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cb14f96659eaeae4205c67e15c90a55e" rel="nofollow" data-download="{"attachment_id":55994478,"asset_id":36103397,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/55994478/download_file?st=MTczMjc4NTE1Myw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="50146524" href="https://independent.academia.edu/LCastroMercado">Luis Gabriel Castro Mercado</a><script data-card-contents-for-user="50146524" type="text/json">{"id":50146524,"first_name":"Luis Gabriel","last_name":"Castro Mercado","domain_name":"independent","page_name":"LCastroMercado","display_name":"Luis Gabriel Castro Mercado","profile_url":"https://independent.academia.edu/LCastroMercado?f_ri=18533","photo":"https://0.academia-photos.com/50146524/27149694/25556896/s65_luis_gabriel.castro_mercado.jpg"}</script></span></span></li><li class="js-paper-rank-work_36103397 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="36103397"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 36103397, container: ".js-paper-rank-work_36103397", }); 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Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.","downloadable_attachments":[{"id":55994478,"asset_id":36103397,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":50146524,"first_name":"Luis Gabriel","last_name":"Castro Mercado","domain_name":"independent","page_name":"LCastroMercado","display_name":"Luis Gabriel Castro Mercado","profile_url":"https://independent.academia.edu/LCastroMercado?f_ri=18533","photo":"https://0.academia-photos.com/50146524/27149694/25556896/s65_luis_gabriel.castro_mercado.jpg"}],"research_interests":[{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false},{"id":24706,"name":"Innate immunity","url":"https://www.academia.edu/Documents/in/Innate_immunity?f_ri=18533","nofollow":false},{"id":37834,"name":"Western 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data-card-contents-for-ri="35938" type="text/json">{"id":35938,"name":"Digital Image Processing","url":"https://www.academia.edu/Documents/in/Digital_Image_Processing?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=31236588]'), work: {"id":31236588,"title":"Nodal swelling produced by ciguatoxin-induced selective activation of sodium channels in myelinated nerve fibers","created_at":"2017-02-05T01:48:42.273-08:00","url":"https://www.academia.edu/31236588/Nodal_swelling_produced_by_ciguatoxin_induced_selective_activation_of_sodium_channels_in_myelinated_nerve_fibers?f_ri=18533","dom_id":"work_31236588","summary":null,"downloadable_attachments":[{"id":51645117,"asset_id":31236588,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":59182896,"first_name":"Jordi","last_name":"MOLGO","domain_name":"independent","page_name":"JMOLGO","display_name":"Jordi 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Frequency","url":"https://www.academia.edu/Documents/in/High_Frequency?f_ri=18533"},{"id":214992,"name":"Ciguatoxins","url":"https://www.academia.edu/Documents/in/Ciguatoxins?f_ri=18533"},{"id":386342,"name":"Sodium","url":"https://www.academia.edu/Documents/in/Sodium?f_ri=18533"},{"id":394778,"name":"Sodium channel","url":"https://www.academia.edu/Documents/in/Sodium_channel?f_ri=18533"},{"id":549122,"name":"Confocal Laser Scanning Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Laser_Scanning_Microscopy?f_ri=18533"},{"id":561957,"name":"Sciatic Nerve","url":"https://www.academia.edu/Documents/in/Sciatic_Nerve?f_ri=18533"},{"id":572924,"name":"Tetrodotoxin","url":"https://www.academia.edu/Documents/in/Tetrodotoxin?f_ri=18533"},{"id":666669,"name":"Action potential","url":"https://www.academia.edu/Documents/in/Action_potential?f_ri=18533"},{"id":955727,"name":"Action 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u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/830125/Conidial_anastomosis_tubes_in_filamentous_fungi">Conidial anastomosis tubes in filamentous fungi</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/830125" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1c02718f696930ead8aae14f1696ae96" rel="nofollow" data-download="{"attachment_id":51222346,"asset_id":830125,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm 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Roca","profile_url":"https://independent.academia.edu/GabrielaRoca13?f_ri=18533","photo":"https://0.academia-photos.com/317157/94102/11562919/s65_gabriela.roca.jpg"}</script></span></span></li><li class="js-paper-rank-work_830125 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="830125"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 830125, container: ".js-paper-rank-work_830125", }); });</script></li><li class="js-percentile-work_830125 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 830125; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_830125"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_830125 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="830125"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 830125; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=830125]").text(description); $(".js-view-count-work_830125").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_830125").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 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class="InlineList-item-text" data-has-card-for-ri="73614" href="https://www.academia.edu/Documents/in/Cell_fusion">Cell fusion</a>, <script data-card-contents-for-ri="73614" type="text/json">{"id":73614,"name":"Cell fusion","url":"https://www.academia.edu/Documents/in/Cell_fusion?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="73617" href="https://www.academia.edu/Documents/in/Colletotrichum">Colletotrichum</a><script data-card-contents-for-ri="73617" type="text/json">{"id":73617,"name":"Colletotrichum","url":"https://www.academia.edu/Documents/in/Colletotrichum?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=830125]'), work: {"id":830125,"title":"Conidial anastomosis tubes in filamentous 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fusion","url":"https://www.academia.edu/Documents/in/Cell_fusion?f_ri=18533","nofollow":false},{"id":73617,"name":"Colletotrichum","url":"https://www.academia.edu/Documents/in/Colletotrichum?f_ri=18533","nofollow":false},{"id":128413,"name":"Density dependence","url":"https://www.academia.edu/Documents/in/Density_dependence?f_ri=18533"},{"id":435535,"name":"Filamentous fungi","url":"https://www.academia.edu/Documents/in/Filamentous_fungi?f_ri=18533"},{"id":595078,"name":"Neurospora","url":"https://www.academia.edu/Documents/in/Neurospora?f_ri=18533"},{"id":988970,"name":"MAP Kinase","url":"https://www.academia.edu/Documents/in/MAP_Kinase?f_ri=18533"},{"id":1706341,"name":"Gene Transfer","url":"https://www.academia.edu/Documents/in/Gene_Transfer?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_4343877" data-work_id="4343877" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/4343877/Uterine_and_Placental_Angiogenesis_in_the_Australian_Skinks_Ctenotus_taeniolatus_and_Saiphos_equalis">Uterine and Placental Angiogenesis in the Australian Skinks, Ctenotus taeniolatus, and Saiphos equalis</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The evolution of viviparity requires modifications to multiple integrated physiological features to support embryonic development during pregnancy. Embryonic growth during pregnancy is dependent upon the capacity of the uterine vascular... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_4343877" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The evolution of viviparity requires modifications to multiple integrated physiological features to support embryonic development during pregnancy. Embryonic growth during pregnancy is dependent upon the capacity of the uterine vascular system to satisfy increasing embryonic oxygen demand throughout gestation. We tested the hypothesis that total surface area of uterine blood vessels increases in concert with embryonic growth, and hence its oxygen demand, during gestation. We used immunofluorescence and laser-scanning confocal microscopy to quantify uterine microvascular density and morphology during gestation in the oviparous skink Ctenotus taeniolatus and in Saiphos equalis, a skink species with prolonged egg retention. For C. taeniolatus, vessel density (Nv) and vessel length-density (Lv) in the embryonic hemisphere of the uterus is 23% and 17% less, respectively, than that of S. equalis and vascular surface-area does not differ as a function of embryo stage. For S. equalis, overall Nv, Lv, and vessel diameter (Dv), does not change during the first half of gestation but increases by 36% (Nv), 44% (Lv), and 60% (Dv) by near-term embryo stages late in gestation. The chorioallantoic membrane of S. equalis increases in absolute size but vascular density does not differ as a function of embryo stage. The marked increase in uterine vascular density during late gestation coincides with the phase of rapid growth in embryo mass and concomitant increase in metabolic rate. Expansion of the uterine vascular bed in concert with embryo size and metabolism is likely to be an important transitional step in the evolution of viviparity. Anat Rec, 293:829–838, 2010. © 2010 Wiley-Liss, Inc.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/4343877" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="18c2d78fe03298b054bbcafd0420dad0" rel="nofollow" data-download="{"attachment_id":49923970,"asset_id":4343877,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/49923970/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="5300214" href="https://sydney.academia.edu/Frank">Frank Manconi</a><script data-card-contents-for-user="5300214" type="text/json">{"id":5300214,"first_name":"Frank","last_name":"Manconi","domain_name":"sydney","page_name":"Frank","display_name":"Frank Manconi","profile_url":"https://sydney.academia.edu/Frank?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_4343877 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="4343877"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 4343877, container: ".js-paper-rank-work_4343877", }); 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Embryonic growth during pregnancy is dependent upon the capacity of the uterine vascular system to satisfy increasing embryonic oxygen demand throughout gestation. We tested the hypothesis that total surface area of uterine blood vessels increases in concert with embryonic growth, and hence its oxygen demand, during gestation. We used immunofluorescence and laser-scanning confocal microscopy to quantify uterine microvascular density and morphology during gestation in the oviparous skink Ctenotus taeniolatus and in Saiphos equalis, a skink species with prolonged egg retention. For C. taeniolatus, vessel density (Nv) and vessel length-density (Lv) in the embryonic hemisphere of the uterus is 23% and 17% less, respectively, than that of S. equalis and vascular surface-area does not differ as a function of embryo stage. For S. equalis, overall Nv, Lv, and vessel diameter (Dv), does not change during the first half of gestation but increases by 36% (Nv), 44% (Lv), and 60% (Dv) by near-term embryo stages late in gestation. The chorioallantoic membrane of S. equalis increases in absolute size but vascular density does not differ as a function of embryo stage. The marked increase in uterine vascular density during late gestation coincides with the phase of rapid growth in embryo mass and concomitant increase in metabolic rate. Expansion of the uterine vascular bed in concert with embryo size and metabolism is likely to be an important transitional step in the evolution of viviparity. Anat Rec, 293:829–838, 2010. © 2010 Wiley-Liss, Inc.","downloadable_attachments":[{"id":49923970,"asset_id":4343877,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":5300214,"first_name":"Frank","last_name":"Manconi","domain_name":"sydney","page_name":"Frank","display_name":"Frank Manconi","profile_url":"https://sydney.academia.edu/Frank?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=18533","nofollow":false},{"id":76061,"name":"Placenta","url":"https://www.academia.edu/Documents/in/Placenta?f_ri=18533","nofollow":false},{"id":92463,"name":"Lizards","url":"https://www.academia.edu/Documents/in/Lizards?f_ri=18533","nofollow":false},{"id":323597,"name":"Fluorescent Antibody Technique","url":"https://www.academia.edu/Documents/in/Fluorescent_Antibody_Technique?f_ri=18533"},{"id":352300,"name":"Embryonic Development","url":"https://www.academia.edu/Documents/in/Embryonic_Development?f_ri=18533"},{"id":405386,"name":"Record","url":"https://www.academia.edu/Documents/in/Record?f_ri=18533"},{"id":987079,"name":"Uterus","url":"https://www.academia.edu/Documents/in/Uterus?f_ri=18533"},{"id":2534790,"name":"Chorioallantoic Membrane","url":"https://www.academia.edu/Documents/in/Chorioallantoic_Membrane?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27553203 coauthored" data-work_id="27553203" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/27553203/Measurement_and_mapping_of_pH_in_hydrating_pharmaceutical_pellets_using_confocal_laser_scanning_microscopy">Measurement and mapping of pH in hydrating pharmaceutical pellets using confocal laser scanning microscopy</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">pH modifiers are often used to promote drug solubility/ stability in dosage forms, but predicting the extent and duration of internal pH modification is difficult. Here, a noninvasive technique is developed for the spatial and temporal... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_27553203" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">pH modifiers are often used to promote drug solubility/ stability in dosage forms, but predicting the extent and duration of internal pH modification is difficult. Here, a noninvasive technique is developed for the spatial and temporal mapping of pH in a hydrated pharmaceutical pellet, within a pH range appropriate for microenvironmental pH control by weak acids. Confocal dual excitation imaging (Ex 488/Ex 568) of pellets containing a single, soluble, pH-sensitive fluorophore with cross-validation from a pH microelectrode. The technique was used to investigate the changing pH distribution in hydrating pellets containing two weak acids of differing solubility. The algorithm developed provided pH measurements over the range pH 3.5-5.5 with a typical accuracy of 0.1 pH units and with excellent correlation with pH microelectrode measurements. The method showed how pellets containing 25%w/w tartaric acid exhibited a rapid but transient fall in internal pH, in contrast to a slower more pr...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/27553203" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ff5b53dcbc94854c4428584d89c41cad" rel="nofollow" data-download="{"attachment_id":47807139,"asset_id":27553203,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47807139/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="51720756" href="https://independent.academia.edu/StephenHibberd">Stephen Hibberd</a><script data-card-contents-for-user="51720756" type="text/json">{"id":51720756,"first_name":"Stephen","last_name":"Hibberd","domain_name":"independent","page_name":"StephenHibberd","display_name":"Stephen Hibberd","profile_url":"https://independent.academia.edu/StephenHibberd?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-27553203">+1</span><div class="hidden js-additional-users-27553203"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://mpa.academia.edu/JoanneWhetstone">Joanne Whetstone</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-27553203'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-27553203').html(); 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Here, a noninvasive technique is developed for the spatial and temporal mapping of pH in a hydrated pharmaceutical pellet, within a pH range appropriate for microenvironmental pH control by weak acids. Confocal dual excitation imaging (Ex 488/Ex 568) of pellets containing a single, soluble, pH-sensitive fluorophore with cross-validation from a pH microelectrode. The technique was used to investigate the changing pH distribution in hydrating pellets containing two weak acids of differing solubility. The algorithm developed provided pH measurements over the range pH 3.5-5.5 with a typical accuracy of 0.1 pH units and with excellent correlation with pH microelectrode measurements. The method showed how pellets containing 25%w/w tartaric acid exhibited a rapid but transient fall in internal pH, in contrast to a slower more pr...","downloadable_attachments":[{"id":47807139,"asset_id":27553203,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":51720756,"first_name":"Stephen","last_name":"Hibberd","domain_name":"independent","page_name":"StephenHibberd","display_name":"Stephen Hibberd","profile_url":"https://independent.academia.edu/StephenHibberd?f_ri=18533","photo":"/images/s65_no_pic.png"},{"id":51729752,"first_name":"Joanne","last_name":"Whetstone","domain_name":"mpa","page_name":"JoanneWhetstone","display_name":"Joanne Whetstone","profile_url":"https://mpa.academia.edu/JoanneWhetstone?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":165,"name":"Pathology","url":"https://www.academia.edu/Documents/in/Pathology?f_ri=18533","nofollow":false},{"id":2184,"name":"Electron Microscopy","url":"https://www.academia.edu/Documents/in/Electron_Microscopy?f_ri=18533","nofollow":false},{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=18533","nofollow":false},{"id":7968,"name":"Prediction","url":"https://www.academia.edu/Documents/in/Prediction?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533"},{"id":37959,"name":"Cellulose","url":"https://www.academia.edu/Documents/in/Cellulose?f_ri=18533"},{"id":41747,"name":"Dosage Form","url":"https://www.academia.edu/Documents/in/Dosage_Form?f_ri=18533"},{"id":84278,"name":"Ph","url":"https://www.academia.edu/Documents/in/Ph?f_ri=18533"},{"id":94514,"name":"Pellet","url":"https://www.academia.edu/Documents/in/Pellet?f_ri=18533"},{"id":95655,"name":"Pharmaceutical","url":"https://www.academia.edu/Documents/in/Pharmaceutical?f_ri=18533"},{"id":113460,"name":"Laser Scanning","url":"https://www.academia.edu/Documents/in/Laser_Scanning?f_ri=18533"},{"id":291387,"name":"Mathematical Model","url":"https://www.academia.edu/Documents/in/Mathematical_Model?f_ri=18533"},{"id":548191,"name":"Microelectrode","url":"https://www.academia.edu/Documents/in/Microelectrode?f_ri=18533"},{"id":549122,"name":"Confocal Laser Scanning Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Laser_Scanning_Microscopy?f_ri=18533"},{"id":1137254,"name":"Hydrogen-Ion Concentration","url":"https://www.academia.edu/Documents/in/Hydrogen-Ion_Concentration?f_ri=18533"},{"id":2251042,"name":"pH control","url":"https://www.academia.edu/Documents/in/pH_control?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_27272610" data-work_id="27272610" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title 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href="https://www.academia.edu/12667393/Cellular_localization_of_TRPC5_in_the_substantia_nigra_of_rat">Cellular localization of TRPC5 in the substantia nigra of rat</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Transient receptor potential channels (TRPC) are plasma membrane, non-selective cationic channels and have been proposed as candidates involved in the regulation of cellular Ca2+ influx. TRPC are involved in metabotropic glutamate... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_12667393" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Transient receptor potential channels (TRPC) are plasma membrane, non-selective cationic channels and have been proposed as candidates involved in the regulation of cellular Ca2+ influx. TRPC are involved in metabotropic glutamate receptor (mGluR)-mediated excitatory post-synaptic currents (EPSCs) in the dopaminergic neurons of the substantia nigra. We previously observed several subtypes of TRPC to be expressed at an mRNA level in</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/12667393" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="827a23089a3c64161fa2bd39606b90a0" rel="nofollow" data-download="{"attachment_id":46017990,"asset_id":12667393,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46017990/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="31667995" href="https://independent.academia.edu/FrancescaFusco">Francesca Fusco</a><script data-card-contents-for-user="31667995" type="text/json">{"id":31667995,"first_name":"Francesca","last_name":"Fusco","domain_name":"independent","page_name":"FrancescaFusco","display_name":"Francesca Fusco","profile_url":"https://independent.academia.edu/FrancescaFusco?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_12667393 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="12667393"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 12667393, container: ".js-paper-rank-work_12667393", }); 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js-work-card work_17873350" data-work_id="17873350" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/17873350/Pattern_of_growth_and_respiratory_activity_of_Saccharomyces_cerevisiae_bakers_yeast_cells_growing_entrapped_in_an_insolubilized_gelatin_gel">Pattern of growth and respiratory activity of Saccharomyces cerevisiae (baker's yeast) cells growing entrapped in an insolubilized gelatin gel</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_17873350" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Yeast cells immobilized by entrapment in an insolubilized gelatin gel have been investigated with regard to their pattern of growth and respiratory activity. Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for both a lower specific growth rate of immobilized cells with respect to that of free ones, and a heterogeneous biomass distribution, with progressively increased cellular density from the inside to the outside of the matrix. The spatial organization of the growing cells leads to the formation of a biofilm perfectly adherent to the surface of the matrix. Measurements of the oxygen-uptake rate of the immobilized cells, after having been released from the matrix by trypsin digestion, and confocal microscopy of cells stained with Rhodamine 123 demonstrate the occurrence of a gradient of respiratory activity throughout the immobilized culture.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/17873350" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="64acbeaa7ddbb0062a142588f06d41b6" rel="nofollow" data-download="{"attachment_id":39756234,"asset_id":17873350,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/39756234/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37785024" href="https://independent.academia.edu/mariamathuet">maria mathuet</a><script data-card-contents-for-user="37785024" type="text/json">{"id":37785024,"first_name":"maria","last_name":"mathuet","domain_name":"independent","page_name":"mariamathuet","display_name":"maria mathuet","profile_url":"https://independent.academia.edu/mariamathuet?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_17873350 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="17873350"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 17873350, container: ".js-paper-rank-work_17873350", }); 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Mass-transfer resistance offered by the matrix and growth of the entrapped cells determine a gradient of nutrients throughout the gel which is responsible for both a lower specific growth rate of immobilized cells with respect to that of free ones, and a heterogeneous biomass distribution, with progressively increased cellular density from the inside to the outside of the matrix. The spatial organization of the growing cells leads to the formation of a biofilm perfectly adherent to the surface of the matrix. Measurements of the oxygen-uptake rate of the immobilized cells, after having been released from the matrix by trypsin digestion, and confocal microscopy of cells stained with Rhodamine 123 demonstrate the occurrence of a gradient of respiratory activity throughout the immobilized culture.","downloadable_attachments":[{"id":39756234,"asset_id":17873350,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":37785024,"first_name":"maria","last_name":"mathuet","domain_name":"independent","page_name":"mariamathuet","display_name":"maria mathuet","profile_url":"https://independent.academia.edu/mariamathuet?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=18533","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=18533","nofollow":false},{"id":7700,"name":"Fluorescence Microscopy","url":"https://www.academia.edu/Documents/in/Fluorescence_Microscopy?f_ri=18533","nofollow":false},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533"},{"id":38650,"name":"Cell Division","url":"https://www.academia.edu/Documents/in/Cell_Division?f_ri=18533"},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=18533"},{"id":49123,"name":"Saccharomyces cerevisiae","url":"https://www.academia.edu/Documents/in/Saccharomyces_cerevisiae?f_ri=18533"},{"id":190525,"name":"Gelatin","url":"https://www.academia.edu/Documents/in/Gelatin?f_ri=18533"},{"id":205584,"name":"Solubility","url":"https://www.academia.edu/Documents/in/Solubility?f_ri=18533"},{"id":284907,"name":"Gels","url":"https://www.academia.edu/Documents/in/Gels?f_ri=18533"},{"id":294621,"name":"Rhodamine B","url":"https://www.academia.edu/Documents/in/Rhodamine_B?f_ri=18533"},{"id":1193624,"name":"Oxygen Consumption","url":"https://www.academia.edu/Documents/in/Oxygen_Consumption?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_66804197" data-work_id="66804197" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/66804197/Small_Fibers_Peripheral_Neuropathy_in_Wilson_Disease_an_In_Vivo_Documentation_by_Corneal_Confocal_Microscopy">Small Fibers Peripheral Neuropathy in Wilson Disease: an In Vivo Documentation by Corneal Confocal Microscopy</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Purpose: Wilson disease (WD) is a disorder of hepatic copper metabolism leading to copper accumulation in hepatocytes and in extrahepatic organs, as the brain and the cornea. The aim of this study was to investigate central corneal... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_66804197" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Purpose: Wilson disease (WD) is a disorder of hepatic copper metabolism leading to copper accumulation in hepatocytes and in extrahepatic organs, as the brain and the cornea. The aim of this study was to investigate central corneal changes and in particular to assess the parameters of corneal sub-basal nerve plexus (SBNP) in patients affected by WD, using corneal confocal microscopy (CCM). Twenty-four patients affected by WD and 24 healthy control subjects were incuded in this cross-sectional comparative study. One eye of each subject was examined to quantify different corneal parameters. Mean cell diameter and mean cell density of the epithelium; number of fibers (NF), nerve fiber length density (NFLD), number of branchings (NBr), number of beadings (NBe) and fiber tortuosity (FT) of the SBNP; mean cell density of keratocytes of the anterior, medium and posterior stroma and mean cell density, polimegatism and pleomorphism of the endothelium and central corneal sensitivity were anal...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66804197" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="a31b38969b72a96f6a0db85607d022dc" rel="nofollow" data-download="{"attachment_id":77855602,"asset_id":66804197,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/77855602/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="52495617" href="https://independent.academia.edu/MariannaBerton">Marianna Berton</a><script data-card-contents-for-user="52495617" type="text/json">{"id":52495617,"first_name":"Marianna","last_name":"Berton","domain_name":"independent","page_name":"MariannaBerton","display_name":"Marianna Berton","profile_url":"https://independent.academia.edu/MariannaBerton?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_66804197 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="66804197"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 66804197, container: ".js-paper-rank-work_66804197", }); 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$(".js-view-count[data-work-id=66804197]").text(description); $(".js-view-count-work_66804197").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_66804197").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="66804197"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">13</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="17721" href="https://www.academia.edu/Documents/in/Cornea">Cornea</a>, <script data-card-contents-for-ri="17721" type="text/json">{"id":17721,"name":"Cornea","url":"https://www.academia.edu/Documents/in/Cornea?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="18533" href="https://www.academia.edu/Documents/in/Confocal_Microscopy">Confocal Microscopy</a>, <script data-card-contents-for-ri="18533" type="text/json">{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a>, <script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a><script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=66804197]'), work: {"id":66804197,"title":"Small Fibers Peripheral Neuropathy in Wilson Disease: an In Vivo Documentation by Corneal Confocal Microscopy","created_at":"2022-01-01T10:23:04.380-08:00","url":"https://www.academia.edu/66804197/Small_Fibers_Peripheral_Neuropathy_in_Wilson_Disease_an_In_Vivo_Documentation_by_Corneal_Confocal_Microscopy?f_ri=18533","dom_id":"work_66804197","summary":"Purpose: Wilson disease (WD) is a disorder of hepatic copper metabolism leading to copper accumulation in hepatocytes and in extrahepatic organs, as the brain and the cornea. The aim of this study was to investigate central corneal changes and in particular to assess the parameters of corneal sub-basal nerve plexus (SBNP) in patients affected by WD, using corneal confocal microscopy (CCM). Twenty-four patients affected by WD and 24 healthy control subjects were incuded in this cross-sectional comparative study. One eye of each subject was examined to quantify different corneal parameters. Mean cell diameter and mean cell density of the epithelium; number of fibers (NF), nerve fiber length density (NFLD), number of branchings (NBr), number of beadings (NBe) and fiber tortuosity (FT) of the SBNP; mean cell density of keratocytes of the anterior, medium and posterior stroma and mean cell density, polimegatism and pleomorphism of the endothelium and central corneal sensitivity were anal...","downloadable_attachments":[{"id":77855602,"asset_id":66804197,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":52495617,"first_name":"Marianna","last_name":"Berton","domain_name":"independent","page_name":"MariannaBerton","display_name":"Marianna Berton","profile_url":"https://independent.academia.edu/MariannaBerton?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":17721,"name":"Cornea","url":"https://www.academia.edu/Documents/in/Cornea?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal 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Studies","url":"https://www.academia.edu/Documents/in/Cross_Sectional_Studies?f_ri=18533"},{"id":1739636,"name":"Hepatolenticular degeneration","url":"https://www.academia.edu/Documents/in/Hepatolenticular_degeneration?f_ri=18533"},{"id":2796077,"name":"Cell count","url":"https://www.academia.edu/Documents/in/Cell_count?f_ri=18533"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_66581137" data-work_id="66581137" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/66581137/Iron_loaded_synthetic_chrysotile_a_new_model_solid_for_studying_the_role_of_iron_in_asbestos_toxicity">Iron-loaded synthetic chrysotile: a new model solid for studying the role of iron in asbestos toxicity</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/66581137" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d3fbe683223ddbe18407c49b272230c3" rel="nofollow" data-download="{"attachment_id":77716326,"asset_id":66581137,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" 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Toxicology","url":"https://www.academia.edu/Documents/in/Food_and_Chemical_Toxicology?f_ri=18533"},{"id":1446339,"name":"Malondialdehyde","url":"https://www.academia.edu/Documents/in/Malondialdehyde?f_ri=18533"},{"id":1954742,"name":"Pentose Phosphate Pathway","url":"https://www.academia.edu/Documents/in/Pentose_Phosphate_Pathway?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_63191805" data-work_id="63191805" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/63191805/Adult_human_buccal_epithelial_stem_cells_identification_ex_vivo_expansion_and_transplantation_for_corneal_surface_reconstruction">Adult human buccal epithelial stem cells: identification, ex-vivo expansion, and transplantation for corneal surface reconstruction</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">To identify adult human buccal epithelial stem cells (SCs) on the basis of two parameters (high p63 expression and greater nucleus/cytoplasmic (N/C) ratio) and to evaluate clinical efficacy of ex-vivo expanded autologous epithelium in... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_63191805" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">To identify adult human buccal epithelial stem cells (SCs) on the basis of two parameters (high p63 expression and greater nucleus/cytoplasmic (N/C) ratio) and to evaluate clinical efficacy of ex-vivo expanded autologous epithelium in bilateral limbal SC-deficient (LSCD) patients. The epithelial cells were isolated from buccal biopsy and cultured on human amnion in culture inserts with 3T3 feeder layer. The SCs were identified on the basis of two-parameter analysis using confocal microscopy, surface markers, and colony-forming efficiency (CFE). The cultured epithelium was transplanted in 10 LSCD patients followed by penetrating keratoplasty in 4 patients. The clinical outcome was followed up to 3 years. A distinct population (3.0±1.7%) of small cells expressing high levels of p63 with greater N/C ratio was observed in buccal epithelium. The N/C ratio was found to be more appropriate than cell diameter for two-parameter analysis. These cells located in the basal layer were negative f...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/63191805" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ecb0c59ca1a716777a178596d9f903de" rel="nofollow" data-download="{"attachment_id":75697682,"asset_id":63191805,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75697682/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="14424239" href="https://independent.academia.edu/ChidambaranathanGowripriya">Chidambaranathan Gowri priya</a><script data-card-contents-for-user="14424239" type="text/json">{"id":14424239,"first_name":"Chidambaranathan","last_name":"Gowri priya","domain_name":"independent","page_name":"ChidambaranathanGowripriya","display_name":"Chidambaranathan Gowri priya","profile_url":"https://independent.academia.edu/ChidambaranathanGowripriya?f_ri=18533","photo":"https://0.academia-photos.com/14424239/10675404/11917685/s65_chidambaranathan.gowri_priya.jpg"}</script></span></span></li><li class="js-paper-rank-work_63191805 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="63191805"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 63191805, container: ".js-paper-rank-work_63191805", }); 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$(".js-view-count[data-work-id=63191805]").text(description); $(".js-view-count-work_63191805").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_63191805").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="63191805"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">15</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="1290" href="https://www.academia.edu/Documents/in/Immunology">Immunology</a>, <script data-card-contents-for-ri="1290" type="text/json">{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="18533" href="https://www.academia.edu/Documents/in/Confocal_Microscopy">Confocal Microscopy</a>, <script data-card-contents-for-ri="18533" type="text/json">{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="18608" href="https://www.academia.edu/Documents/in/Adult_Stem_Cells">Adult Stem Cells</a>, <script data-card-contents-for-ri="18608" type="text/json">{"id":18608,"name":"Adult Stem Cells","url":"https://www.academia.edu/Documents/in/Adult_Stem_Cells?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="22506" href="https://www.academia.edu/Documents/in/Adolescent">Adolescent</a><script data-card-contents-for-ri="22506" type="text/json">{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=63191805]'), work: {"id":63191805,"title":"Adult human buccal epithelial stem cells: identification, ex-vivo expansion, and transplantation for corneal surface reconstruction","created_at":"2021-12-04T06:50:36.310-08:00","url":"https://www.academia.edu/63191805/Adult_human_buccal_epithelial_stem_cells_identification_ex_vivo_expansion_and_transplantation_for_corneal_surface_reconstruction?f_ri=18533","dom_id":"work_63191805","summary":"To identify adult human buccal epithelial stem cells (SCs) on the basis of two parameters (high p63 expression and greater nucleus/cytoplasmic (N/C) ratio) and to evaluate clinical efficacy of ex-vivo expanded autologous epithelium in bilateral limbal SC-deficient (LSCD) patients. The epithelial cells were isolated from buccal biopsy and cultured on human amnion in culture inserts with 3T3 feeder layer. The SCs were identified on the basis of two-parameter analysis using confocal microscopy, surface markers, and colony-forming efficiency (CFE). The cultured epithelium was transplanted in 10 LSCD patients followed by penetrating keratoplasty in 4 patients. The clinical outcome was followed up to 3 years. A distinct population (3.0±1.7%) of small cells expressing high levels of p63 with greater N/C ratio was observed in buccal epithelium. The N/C ratio was found to be more appropriate than cell diameter for two-parameter analysis. These cells located in the basal layer were negative f...","downloadable_attachments":[{"id":75697682,"asset_id":63191805,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":14424239,"first_name":"Chidambaranathan","last_name":"Gowri priya","domain_name":"independent","page_name":"ChidambaranathanGowripriya","display_name":"Chidambaranathan Gowri priya","profile_url":"https://independent.academia.edu/ChidambaranathanGowripriya?f_ri=18533","photo":"https://0.academia-photos.com/14424239/10675404/11917685/s65_chidambaranathan.gowri_priya.jpg"}],"research_interests":[{"id":1290,"name":"Immunology","url":"https://www.academia.edu/Documents/in/Immunology?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false},{"id":18608,"name":"Adult Stem Cells","url":"https://www.academia.edu/Documents/in/Adult_Stem_Cells?f_ri=18533","nofollow":false},{"id":22506,"name":"Adolescent","url":"https://www.academia.edu/Documents/in/Adolescent?f_ri=18533","nofollow":false},{"id":23163,"name":"Stem Cell","url":"https://www.academia.edu/Documents/in/Stem_Cell?f_ri=18533"},{"id":64933,"name":"Child","url":"https://www.academia.edu/Documents/in/Child?f_ri=18533"},{"id":133057,"name":"Young Adult","url":"https://www.academia.edu/Documents/in/Young_Adult?f_ri=18533"},{"id":189056,"name":"Surface Reconstruction","url":"https://www.academia.edu/Documents/in/Surface_Reconstruction?f_ri=18533"},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=18533"},{"id":245634,"name":"Eye","url":"https://www.academia.edu/Documents/in/Eye?f_ri=18533"},{"id":289271,"name":"Aged","url":"https://www.academia.edu/Documents/in/Aged?f_ri=18533"},{"id":320785,"name":"Ex vivo expansion","url":"https://www.academia.edu/Documents/in/Ex_vivo_expansion?f_ri=18533"},{"id":379416,"name":"Epithelial cells","url":"https://www.academia.edu/Documents/in/Epithelial_cells?f_ri=18533"},{"id":385863,"name":"Mouth mucosa","url":"https://www.academia.edu/Documents/in/Mouth_mucosa?f_ri=18533"},{"id":1159784,"name":"Penetrating keratoplasty","url":"https://www.academia.edu/Documents/in/Penetrating_keratoplasty?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62462449" data-work_id="62462449" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62462449/Thickness_estimation_of_fluorescent_sections_using_a_CSLM">Thickness estimation of fluorescent sections using a CSLM</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62462449" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cfcd49a75a3a646231372396ebdc9291" rel="nofollow" data-download="{"attachment_id":75218324,"asset_id":62462449,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/75218324/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa 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data-has-card-for-ri="56" href="https://www.academia.edu/Documents/in/Materials_Engineering">Materials Engineering</a>, <script data-card-contents-for-ri="56" type="text/json">{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="505" href="https://www.academia.edu/Documents/in/Condensed_Matter_Physics">Condensed Matter Physics</a>, <script data-card-contents-for-ri="505" type="text/json">{"id":505,"name":"Condensed Matter Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="3091" href="https://www.academia.edu/Documents/in/Microscopy">Microscopy</a>, <script data-card-contents-for-ri="3091" type="text/json">{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="7698" href="https://www.academia.edu/Documents/in/Fluorescence">Fluorescence</a><script data-card-contents-for-ri="7698" type="text/json">{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=62462449]'), work: {"id":62462449,"title":"Thickness estimation of fluorescent sections using a 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Physics","url":"https://www.academia.edu/Documents/in/Condensed_Matter_Physics?f_ri=18533","nofollow":false},{"id":3091,"name":"Microscopy","url":"https://www.academia.edu/Documents/in/Microscopy?f_ri=18533","nofollow":false},{"id":7698,"name":"Fluorescence","url":"https://www.academia.edu/Documents/in/Fluorescence?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533"},{"id":151141,"name":"Refractive Index","url":"https://www.academia.edu/Documents/in/Refractive_Index?f_ri=18533"},{"id":1112728,"name":"Point Spread Function","url":"https://www.academia.edu/Documents/in/Point_Spread_Function?f_ri=18533"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=18533"},{"id":1773970,"name":"Section","url":"https://www.academia.edu/Documents/in/Section?f_ri=18533"},{"id":2187257,"name":"FWHM","url":"https://www.academia.edu/Documents/in/FWHM?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_61188810" data-work_id="61188810" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/61188810/The_Movement_of_Coiled_Bodies_Visualized_in_Living_Plant_Cells_by_the_Green_Fluorescent_Protein">The Movement of Coiled Bodies Visualized in Living Plant Cells by the Green Fluorescent Protein</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Coiled bodies are nuclear organelles that contain components of at least three RNA-processing pathways: pre-mRNA splicing, histone mRNA 3′- maturation, and pre-rRNA processing. Their function remains unknown. However, it has been... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_61188810" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Coiled bodies are nuclear organelles that contain components of at least three RNA-processing pathways: pre-mRNA splicing, histone mRNA 3′- maturation, and pre-rRNA processing. Their function remains unknown. However, it has been speculated that coiled bodies may be sites of splicing factor assembly and/or recycling, play a role in histone mRNA 3′-processing, or act as nuclear transport or sorting structures. To study the dynamics of coiled bodies in living cells, we have stably expressed a U2B&quot;–green fluorescent protein fusion in tobacco BY-2 cells and in Arabidopsis plants. Time-lapse confocal microscopy has shown that coiled bodies are mobile organelles in plant cells. We have observed movements of coiled bodies in the nucleolus, in the nucleoplasm, and from the periphery of the nucleus into the nucleolus, which suggests a transport function for coiled bodies. 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The method allows the identification of different dynamical patterns such as... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_6652769" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We have developed an automatic method for the analysis and identification of dynamical regimes in intracellular calcium patterns from confocal calcium images. The method allows the identification of different dynamical patterns such as spatially concordant and discordant alternans, irregular behavior or phase-locking regimes such as period doubling or halving. The method can be applied to the analysis of different cardiac pathologies related to anomalies at the cellular level such as ventricular reentrant arrhythmias.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/6652769" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="9a089d8c759f2384b5552f2c382b6af9" rel="nofollow" data-download="{"attachment_id":48766323,"asset_id":6652769,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48766323/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="10796947" href="https://independent.academia.edu/raulbenitez1">raul benitez</a><script data-card-contents-for-user="10796947" type="text/json">{"id":10796947,"first_name":"raul","last_name":"benitez","domain_name":"independent","page_name":"raulbenitez1","display_name":"raul benitez","profile_url":"https://independent.academia.edu/raulbenitez1?f_ri=18533","photo":"https://0.academia-photos.com/10796947/7158891/8062317/s65_raul.benitez.jpg"}</script></span></span></li><li class="js-paper-rank-work_6652769 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="6652769"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 6652769, container: ".js-paper-rank-work_6652769", }); 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The method allows the identification of different dynamical patterns such as spatially concordant and discordant alternans, irregular behavior or phase-locking regimes such as period doubling or halving. 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itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/14538695/The_Chemokine_Receptor_CXCR3_Is_Degraded_following_Internalization_and_Is_Replenished_at_the_Cell_Surface_by_De_Novo_Synthesis_of_Receptor">The Chemokine Receptor CXCR3 Is Degraded following Internalization and Is Replenished at the Cell Surface by De Novo Synthesis of Receptor</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/14538695" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div 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line","url":"https://www.academia.edu/Documents/in/Cell_line?f_ri=18533"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=18533"},{"id":90156,"name":"Endocytosis","url":"https://www.academia.edu/Documents/in/Endocytosis?f_ri=18533"},{"id":161176,"name":"The","url":"https://www.academia.edu/Documents/in/The?f_ri=18533"},{"id":193464,"name":"Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis","url":"https://www.academia.edu/Documents/in/Sodium_Dodecyl_Sulfate-Polyacrylamide_Gel_Electrophoresis?f_ri=18533"},{"id":280237,"name":"T lymphocytes","url":"https://www.academia.edu/Documents/in/T_lymphocytes?f_ri=18533"},{"id":620070,"name":"Transfection","url":"https://www.academia.edu/Documents/in/Transfection?f_ri=18533"},{"id":809881,"name":"Amino Acid Sequence","url":"https://www.academia.edu/Documents/in/Amino_Acid_Sequence?f_ri=18533"},{"id":1217930,"name":"Site-directed 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u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="8501" href="https://www.academia.edu/Documents/in/Phospholipids">Phospholipids</a>, <script data-card-contents-for-ri="8501" type="text/json">{"id":8501,"name":"Phospholipids","url":"https://www.academia.edu/Documents/in/Phospholipids?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="18529" href="https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents">Fluorescent Dyes and Reagents</a>, <script data-card-contents-for-ri="18529" type="text/json">{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="18533" href="https://www.academia.edu/Documents/in/Confocal_Microscopy">Confocal Microscopy</a>, <script data-card-contents-for-ri="18533" type="text/json">{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="21408" href="https://www.academia.edu/Documents/in/Folic_acid">Folic acid</a><script data-card-contents-for-ri="21408" type="text/json">{"id":21408,"name":"Folic acid","url":"https://www.academia.edu/Documents/in/Folic_acid?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=23624235]'), work: {"id":23624235,"title":"Indocyanine Green-Containing Nanostructure as Near Infrared Dual-Functional Targeting Probes for Optical Imaging and Photothermal Therapy","created_at":"2016-03-23T11:08:06.391-07:00","url":"https://www.academia.edu/23624235/Indocyanine_Green_Containing_Nanostructure_as_Near_Infrared_Dual_Functional_Targeting_Probes_for_Optical_Imaging_and_Photothermal_Therapy?f_ri=18533","dom_id":"work_23624235","summary":null,"downloadable_attachments":[{"id":44037880,"asset_id":23624235,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":45629241,"first_name":"Feifan","last_name":"Zhou","domain_name":"independent","page_name":"FeifanZhou","display_name":"Feifan Zhou","profile_url":"https://independent.academia.edu/FeifanZhou?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":8501,"name":"Phospholipids","url":"https://www.academia.edu/Documents/in/Phospholipids?f_ri=18533","nofollow":false},{"id":18529,"name":"Fluorescent Dyes and Reagents","url":"https://www.academia.edu/Documents/in/Fluorescent_Dyes_and_Reagents?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false},{"id":21408,"name":"Folic acid","url":"https://www.academia.edu/Documents/in/Folic_acid?f_ri=18533","nofollow":false},{"id":83120,"name":"Near Infrared Spectroscopy","url":"https://www.academia.edu/Documents/in/Near_Infrared_Spectroscopy?f_ri=18533"},{"id":84760,"name":"Mice","url":"https://www.academia.edu/Documents/in/Mice?f_ri=18533"},{"id":91499,"name":"Glioblastoma","url":"https://www.academia.edu/Documents/in/Glioblastoma?f_ri=18533"},{"id":128132,"name":"Nanostructures","url":"https://www.academia.edu/Documents/in/Nanostructures?f_ri=18533"},{"id":147196,"name":"Monoclonal Antibodies","url":"https://www.academia.edu/Documents/in/Monoclonal_Antibodies?f_ri=18533"},{"id":203765,"name":"Diagnostic Imaging","url":"https://www.academia.edu/Documents/in/Diagnostic_Imaging?f_ri=18533"},{"id":375077,"name":"Photochemotherapy","url":"https://www.academia.edu/Documents/in/Photochemotherapy?f_ri=18533"},{"id":591484,"name":"Laser Therapy","url":"https://www.academia.edu/Documents/in/Laser_Therapy?f_ri=18533"},{"id":1434630,"name":"Polyethylene Glycols","url":"https://www.academia.edu/Documents/in/Polyethylene_Glycols?f_ri=18533"},{"id":2187010,"name":"Molecular Pharmaceutics","url":"https://www.academia.edu/Documents/in/Molecular_Pharmaceutics?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_22062530" data-work_id="22062530" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/22062530/A_novel_POSS_coated_quantum_dot_for_biological_application">A novel POSS-coated quantum dot for biological application</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/22062530" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c163a0dae0bb358fd9ca41fe10f39323" rel="nofollow" data-download="{"attachment_id":42739488,"asset_id":22062530,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen 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Seifalian","profile_url":"https://ucl.academia.edu/AlexanderSeifalian?f_ri=18533","photo":"https://0.academia-photos.com/9126009/2992627/3513188/s65_alexander.seifalian.jpg"}</script></span></span></li><li class="js-paper-rank-work_22062530 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="22062530"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 22062530, container: ".js-paper-rank-work_22062530", }); });</script></li><li class="js-percentile-work_22062530 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 22062530; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = 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data-has-card-for-ri="18533" href="https://www.academia.edu/Documents/in/Confocal_Microscopy">Confocal Microscopy</a>, <script data-card-contents-for-ri="18533" type="text/json">{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="48317" href="https://www.academia.edu/Documents/in/Quantum_Dots">Quantum Dots</a><script data-card-contents-for-ri="48317" type="text/json">{"id":48317,"name":"Quantum Dots","url":"https://www.academia.edu/Documents/in/Quantum_Dots?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=22062530]'), work: {"id":22062530,"title":"A novel POSS-coated quantum dot for biological 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Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false},{"id":48317,"name":"Quantum Dots","url":"https://www.academia.edu/Documents/in/Quantum_Dots?f_ri=18533","nofollow":false},{"id":205584,"name":"Solubility","url":"https://www.academia.edu/Documents/in/Solubility?f_ri=18533"},{"id":398650,"name":"Fourier transform infrared spectroscopy","url":"https://www.academia.edu/Documents/in/Fourier_transform_infrared_spectroscopy?f_ri=18533"},{"id":413194,"name":"Analysis of Variance","url":"https://www.academia.edu/Documents/in/Analysis_of_Variance?f_ri=18533"},{"id":440924,"name":"Surface Properties","url":"https://www.academia.edu/Documents/in/Surface_Properties?f_ri=18533"},{"id":499747,"name":"Cell Shape","url":"https://www.academia.edu/Documents/in/Cell_Shape?f_ri=18533"},{"id":614749,"name":"Cysteine","url":"https://www.academia.edu/Documents/in/Cysteine?f_ri=18533"},{"id":902996,"name":"Organosilicon Compounds","url":"https://www.academia.edu/Documents/in/Organosilicon_Compounds?f_ri=18533"},{"id":956869,"name":"Tellurium","url":"https://www.academia.edu/Documents/in/Tellurium?f_ri=18533"},{"id":1157148,"name":"Cell Survival","url":"https://www.academia.edu/Documents/in/Cell_Survival?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_19231829" data-work_id="19231829" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19231829/Comparative_analysis_and_application_of_fluorescent_protein_tagged_connexins">Comparative analysis and application of fluorescent protein-tagged connexins</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19231829" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="101d7b301ecead8f26e9e4f071d00d10" rel="nofollow" data-download="{"attachment_id":40505366,"asset_id":19231829,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40505366/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39464886" href="https://independent.academia.edu/ShaoQing">Qing Shao</a><script data-card-contents-for-user="39464886" type="text/json">{"id":39464886,"first_name":"Qing","last_name":"Shao","domain_name":"independent","page_name":"ShaoQing","display_name":"Qing Shao","profile_url":"https://independent.academia.edu/ShaoQing?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_19231829 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="19231829"><i class="u-m1x fa fa-bar-chart"></i><strong 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data-work-id="19231829"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 19231829; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=19231829]").text(description); $(".js-view-count-work_19231829").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_19231829").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="19231829"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">11</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="56" href="https://www.academia.edu/Documents/in/Materials_Engineering">Materials Engineering</a>, <script data-card-contents-for-ri="56" type="text/json">{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5462" href="https://www.academia.edu/Documents/in/Gap_Junctions">Gap Junctions</a>, <script data-card-contents-for-ri="5462" type="text/json">{"id":5462,"name":"Gap Junctions","url":"https://www.academia.edu/Documents/in/Gap_Junctions?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="12071" href="https://www.academia.edu/Documents/in/Immunohistochemistry">Immunohistochemistry</a>, <script data-card-contents-for-ri="12071" type="text/json">{"id":12071,"name":"Immunohistochemistry","url":"https://www.academia.edu/Documents/in/Immunohistochemistry?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="18533" href="https://www.academia.edu/Documents/in/Confocal_Microscopy">Confocal Microscopy</a><script data-card-contents-for-ri="18533" type="text/json">{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=19231829]'), work: {"id":19231829,"title":"Comparative analysis and application of fluorescent protein-tagged 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Protein","url":"https://www.academia.edu/Documents/in/Fluorescent_Protein?f_ri=18533"},{"id":620070,"name":"Transfection","url":"https://www.academia.edu/Documents/in/Transfection?f_ri=18533"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_18502168" data-work_id="18502168" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/18502168/Effect_of_the_T_domain_on_intracellular_transport_of_diphtheria_toxin">Effect of the T-domain on intracellular transport of diphtheria toxin</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Subunit B of diphtheria toxin (DT), which consists of two domains: R (receptor-binding) and T (transmembrane), plays an important role in toxin-receptor binding on the cell-targets and in transportation of catalytic subunit A to the cell... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_18502168" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Subunit B of diphtheria toxin (DT), which consists of two domains: R (receptor-binding) and T (transmembrane), plays an important role in toxin-receptor binding on the cell-targets and in transportation of catalytic subunit A to the cell cytosol. Recombinant analogues of the subunit B are promising representatives in the unique class of transporting proteins, able to deliver different types of biologically active molecules to cell cytosol. In the development of these protein constructs understanding of the role of each of the DT fragments in determination of transporting pathways of endocytosed complex toxin-receptor is urgently required. We have studied in this work the T-domain effect on intracellular transport of recombinant fragments of DT. We have compared intracellular transport of the R-domain and the subunit B, the last one was consisted of both R-domain and T-domain. Recombinant fragments of DT used in this work were labeled with fluorescent proteins, which allowed applying...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18502168" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f2be1f67439c76a41aff15c2242cf02d" rel="nofollow" data-download="{"attachment_id":40098733,"asset_id":18502168,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40098733/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="38529829" href="https://independent.academia.edu/DenisKolibo">Denis Kolibo</a><script data-card-contents-for-user="38529829" type="text/json">{"id":38529829,"first_name":"Denis","last_name":"Kolibo","domain_name":"independent","page_name":"DenisKolibo","display_name":"Denis Kolibo","profile_url":"https://independent.academia.edu/DenisKolibo?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_18502168 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="18502168"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 18502168, container: ".js-paper-rank-work_18502168", }); 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Recombinant analogues of the subunit B are promising representatives in the unique class of transporting proteins, able to deliver different types of biologically active molecules to cell cytosol. In the development of these protein constructs understanding of the role of each of the DT fragments in determination of transporting pathways of endocytosed complex toxin-receptor is urgently required. We have studied in this work the T-domain effect on intracellular transport of recombinant fragments of DT. We have compared intracellular transport of the R-domain and the subunit B, the last one was consisted of both R-domain and T-domain. Recombinant fragments of DT used in this work were labeled with fluorescent proteins, which allowed applying...","downloadable_attachments":[{"id":40098733,"asset_id":18502168,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":38529829,"first_name":"Denis","last_name":"Kolibo","domain_name":"independent","page_name":"DenisKolibo","display_name":"Denis Kolibo","profile_url":"https://independent.academia.edu/DenisKolibo?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false},{"id":27784,"name":"Gene expression","url":"https://www.academia.edu/Documents/in/Gene_expression?f_ri=18533","nofollow":false},{"id":57570,"name":"Cercopithecus aethiops","url":"https://www.academia.edu/Documents/in/Cercopithecus_aethiops?f_ri=18533","nofollow":false},{"id":83128,"name":"Escherichia coli","url":"https://www.academia.edu/Documents/in/Escherichia_coli?f_ri=18533"},{"id":123230,"name":"Regression Analysis","url":"https://www.academia.edu/Documents/in/Regression_Analysis?f_ri=18533"},{"id":317801,"name":"Cell nucleus","url":"https://www.academia.edu/Documents/in/Cell_nucleus?f_ri=18533"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding?f_ri=18533"},{"id":1292080,"name":"Vero cells","url":"https://www.academia.edu/Documents/in/Vero_cells?f_ri=18533"},{"id":1457054,"name":"Protein Transport","url":"https://www.academia.edu/Documents/in/Protein_Transport?f_ri=18533"},{"id":1944474,"name":"Diphtheria Toxin","url":"https://www.academia.edu/Documents/in/Diphtheria_Toxin?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_18036728" data-work_id="18036728" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/18036728/Visualisation_of_mycorrhizal_fungal_structures_and_quantification_of_their_surface_area_and_volume_using_laser_scanning_confocal_microscopy">Visualisation of mycorrhizal fungal structures and quantification of their surface area and volume using laser scanning confocal microscopy</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/18036728" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="d0e01937ddf9e787f98884c5cafd8e8f" rel="nofollow" data-download="{"attachment_id":39841661,"asset_id":18036728,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/39841661/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="37974170" href="https://independent.academia.edu/PKolesik">P. 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Kolesik","profile_url":"https://independent.academia.edu/PKolesik?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=18533","nofollow":false},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology?f_ri=18533","nofollow":false},{"id":18533,"name":"Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false},{"id":151141,"name":"Refractive Index","url":"https://www.academia.edu/Documents/in/Refractive_Index?f_ri=18533","nofollow":false},{"id":200716,"name":"Mycorrhiza","url":"https://www.academia.edu/Documents/in/Mycorrhiza?f_ri=18533"},{"id":216315,"name":"Laser Scanning Confocal Microscopy","url":"https://www.academia.edu/Documents/in/Laser_Scanning_Confocal_Microscopy?f_ri=18533"},{"id":386356,"name":"Surface Area","url":"https://www.academia.edu/Documents/in/Surface_Area?f_ri=18533"},{"id":504035,"name":"Three Dimensional","url":"https://www.academia.edu/Documents/in/Three_Dimensional?f_ri=18533"},{"id":1808164,"name":"Arbuscular Mycorrhiza Fungi","url":"https://www.academia.edu/Documents/in/Arbuscular_Mycorrhiza_Fungi?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_62462448" data-work_id="62462448" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/62462448/Confocal_pH_imaging_of_microscopic_specimens_using_fluorescence_lifetimes_and_phase_fluorometry_influence_of_parameter_choice_on_system_performance">Confocal pH imaging of microscopic specimens using fluorescence lifetimes and phase fluorometry: influence of parameter choice on system performance</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/62462448" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="939aa704cfd7b56b0602c76b196980a1" rel="nofollow" data-download="{"attachment_id":75218517,"asset_id":62462448,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" 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Time","url":"https://www.academia.edu/Documents/in/Real_Time?f_ri=18533","nofollow":false},{"id":234860,"name":"Steady state","url":"https://www.academia.edu/Documents/in/Steady_state?f_ri=18533","nofollow":false},{"id":401214,"name":"Endothelial cell","url":"https://www.academia.edu/Documents/in/Endothelial_cell?f_ri=18533"},{"id":571336,"name":"Real Time Monitoring","url":"https://www.academia.edu/Documents/in/Real_Time_Monitoring?f_ri=18533"},{"id":1155801,"name":"Actin Filaments","url":"https://www.academia.edu/Documents/in/Actin_Filaments?f_ri=18533"},{"id":1561715,"name":"Image processing techniques","url":"https://www.academia.edu/Documents/in/Image_processing_techniques?f_ri=18533"},{"id":2493627,"name":"Electrical Impedance","url":"https://www.academia.edu/Documents/in/Electrical_Impedance?f_ri=18533"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_9000436" data-work_id="9000436" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9000436/Equilibrium_cluster_formation_and_gelation">Equilibrium cluster formation and gelation</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We study the formation and growth of equilibrium clusters in a suspension of weakly-charged colloidal particles and small non-adsorbing polymers. The effective potential is characterised by a short-range attraction and a long-range... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_9000436" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We study the formation and growth of equilibrium clusters in a suspension of weakly-charged colloidal particles and small non-adsorbing polymers. The effective potential is characterised by a short-range attraction and a long-range repulsion. The size, shape and local structure of the clusters are studied using three-dimensional particle microscopy. We observe a rapid growth in the mean cluster size and the average number of nearest neighbours approaching the gel boundary.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9000436" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f20d23f9fe9fe421c23bb8d69d0c46f1" rel="nofollow" data-download="{"attachment_id":35313175,"asset_id":9000436,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/35313175/download_file?st=MTczMjc4NTE1NCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="20202269" href="https://independent.academia.edu/rodrigosanchez14">rodrigo sanchez</a><script data-card-contents-for-user="20202269" type="text/json">{"id":20202269,"first_name":"rodrigo","last_name":"sanchez","domain_name":"independent","page_name":"rodrigosanchez14","display_name":"rodrigo sanchez","profile_url":"https://independent.academia.edu/rodrigosanchez14?f_ri=18533","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_9000436 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9000436"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9000436, container: ".js-paper-rank-work_9000436", }); 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We observe a rapid growth in the mean cluster size and the average number of nearest neighbours approaching the gel boundary.","downloadable_attachments":[{"id":35313175,"asset_id":9000436,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":20202269,"first_name":"rodrigo","last_name":"sanchez","domain_name":"independent","page_name":"rodrigosanchez14","display_name":"rodrigo sanchez","profile_url":"https://independent.academia.edu/rodrigosanchez14?f_ri=18533","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":56,"name":"Materials Engineering","url":"https://www.academia.edu/Documents/in/Materials_Engineering?f_ri=18533","nofollow":false},{"id":505,"name":"Condensed Matter 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Microscopy","url":"https://www.academia.edu/Documents/in/Confocal_Microscopy?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="71437" href="https://www.academia.edu/Documents/in/Liver">Liver</a>, <script data-card-contents-for-ri="71437" type="text/json">{"id":71437,"name":"Liver","url":"https://www.academia.edu/Documents/in/Liver?f_ri=18533","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="90156" href="https://www.academia.edu/Documents/in/Endocytosis">Endocytosis</a><script data-card-contents-for-ri="90156" 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